CN116768439A - Method and system for strengthening anaerobic digestion hydrolysis and methane production of cow dung - Google Patents
Method and system for strengthening anaerobic digestion hydrolysis and methane production of cow dung Download PDFInfo
- Publication number
- CN116768439A CN116768439A CN202310911069.9A CN202310911069A CN116768439A CN 116768439 A CN116768439 A CN 116768439A CN 202310911069 A CN202310911069 A CN 202310911069A CN 116768439 A CN116768439 A CN 116768439A
- Authority
- CN
- China
- Prior art keywords
- cow dung
- nbw
- anaerobic digestion
- nano bubble
- methanogenesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 63
- 230000029087 digestion Effects 0.000 title claims abstract description 58
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 210000003608 fece Anatomy 0.000 title claims abstract description 42
- 230000007062 hydrolysis Effects 0.000 title claims abstract description 35
- 238000006460 hydrolysis reaction Methods 0.000 title claims abstract description 35
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 14
- 238000005728 strengthening Methods 0.000 title claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000002101 nanobubble Substances 0.000 claims abstract description 51
- 230000008569 process Effects 0.000 claims abstract description 32
- 238000006243 chemical reaction Methods 0.000 claims abstract description 21
- 238000002156 mixing Methods 0.000 claims abstract description 18
- 239000012153 distilled water Substances 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 230000002708 enhancing effect Effects 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 16
- 230000006872 improvement Effects 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000003134 recirculating effect Effects 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 3
- 229940068196 placebo Drugs 0.000 claims description 3
- 239000000902 placebo Substances 0.000 claims description 3
- 229920001296 polysiloxane Polymers 0.000 claims 1
- 239000007789 gas Substances 0.000 description 12
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- 239000000126 substance Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 229910021529 ammonia Inorganic materials 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 101000937172 Homo sapiens Protein FAN Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000002154 agricultural waste Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- GEHSZWRGPHDXJO-ALELSXGZSA-N coenzyme f420 Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)CC[C@H](C(O)=O)NC(=O)[C@@H](C)O[P@@](O)(=O)OC[C@H](O)[C@@H](O)[C@H](O)CN1C2=CC(O)=CC=C2C=C2C1=NC(=O)NC2=O GEHSZWRGPHDXJO-ALELSXGZSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000010806 kitchen waste Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000009615 deamination Effects 0.000 description 1
- 238000006481 deamination reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000010336 energy treatment Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention belongs to the technical field of anaerobic digestion hydrolysis, and discloses a method and a system for strengthening anaerobic digestion hydrolysis and methane production of cow dung, wherein Air and CO are respectively introduced into a nano bubble generator 2 、N 2 And He gas, the distilled water in the container is recycled to the generator circulation system to prepare different types of nano bubble water; mixing the prepared nano bubble water of different types with cow dung in a ratio of 1:1, bottling, and sealing with a cover special for digestion reaction; and selecting NBW type with better improving effect and economical and practical performance to carry out AD process. The NBs improves the AD system, promotes hydrolysis rate, enhances stability and improves methane yield.
Description
Technical Field
The invention belongs to the technical field of anaerobic digestion hydrolysis, and particularly relates to a method and a system for strengthening anaerobic digestion hydrolysis and methane production of cow dung.
Background
Anaerobic digestion (Anaerobic digestion, AD) is a microorganism-mediated mature technology that can not only solve the problems of waste innocent treatment, etc., but also produce methane-rich biogas. AD is a multiphasic, multistage biochemical process with a host of microorganisms, each stage having a unique individual microorganism with individual nutritional requirements, environmental requirements and sensitivity to environmental stress that are different, yet they coexist in a mixed matrix for serial and orderly metabolic reactions, the metabolites of the last metabolic group often being substrates of the next group. The AD process is very sensitive to disturbance, and the instability of any link can lead to the breakdown of the whole process. For example, (1) slow hydrolysis rates, especially for complex substrates (e.g., lignocellulose, etc.); (2) generating volatile fatty acids (Volatile fatty acids, VFA) (e.g., food waste) from a rapidly biodegradable substrate; (3) producing inhibitory products such as ammonia and hydrogen sulfide; (4) the system stability is poor and highly sensitive to environmental factor changes. Some of these drawbacks may be addressed by employing various pretreatments or additives. However, due to the complex process, high energy and chemical requirements, and the generation of secondary pollutants, the cost is high on an industrial scale and less in practical application.
Through the above analysis, the problems and defects existing in the prior art are as follows: complex process, high energy and chemical requirements, secondary pollutants generation and high cost on an industrial scale.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a method and a system for strengthening anaerobic digestion hydrolysis and methane production of cow dung.
The invention is realized in that a method for strengthening anaerobic digestion hydrolysis and methane production of cow dung comprises the following steps: preparing Nano Bubble Water (NBW); mixing the nano bubble water with cow dung and bottling; the AD procedure is performed by selecting the appropriate NBW type.
Further, the method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung comprises the following steps:
step one, a preparation method of nano bubble water comprises the following steps: air and CO are respectively introduced through a nano bubble generator 2 、N 2 And He gas, recirculating distilled water in the vessel to the generator circulation system for 20 minutes;
step two, mixing the prepared nano bubble water of different types with cow dung in a ratio of 1:1, bottling, and sealing by a cover special for digestion reaction;
and thirdly, selecting an NBW type with better improvement effect, and performing an AD process.
Further, the temperature passing through the nanobubble generator in the first step is 20.+ -. 2 ℃.
Further, in the first step, the pressure is controlled to be 0.4MPa to 0.5MPa during the preparation of NBW.
Further, in the second step, the anaerobic fermentation bottles are respectively of CO type 2 -NBW、He-NBW、N 2 -NBW, air-NBW and placebo DW.
Further, in the second step, the special cover for digestion reaction is provided with two small holes (with the inner diameter of 6 mm), a silica gel tube (with the inner diameter of 6 mm) connects one hole with the air bag for biogas sampling, and a peristaltic pump is used for collecting the digestion sample in the other hole.
Further, in the second step, AD process was performed under conditions of high temperature (55.+ -. 1 ℃) and medium temperature (37.+ -. 1 ℃), respectively.
Further, in the third step, air-NBW type is selected.
In the third step, air-NBW and cow dung are respectively mixed and bottled in a mode of 1:1, 2:1 and 4:1, three corresponding blank control DW: CM=1:1, 2:1 and 4:1 are arranged, and AD processes are respectively carried out under the conditions of high temperature (55+/-1 ℃) and medium temperature (37+/-1 ℃).
Another object of the present invention is to provide an application of a method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung in enhancing anaerobic digestion hydrolysis and improving methane yield of cow dung.
In combination with the technical scheme and the technical problems to be solved, the technical scheme to be protected has the following advantages and positive effects:
firstly, aiming at the problems of complex technology, high energy, high chemical demand, secondary pollutant generation and the like in the prior art, the invention provides a method for improving the AD performance of organic solid waste by using NBs technology, which comprises the steps of firstly respectively introducing Air and CO through a nano bubble generator 2 、N 2 And He gas, the distilled water in the container is recycled to the generator circulation system to prepare different types of nano bubble water; mixing the prepared nano bubble water of different types with cow dung in a ratio of 1:1, bottling, and sealing with a cover special for digestion reaction; and selecting NBW type with better improving effect and economical and practical performance to carry out AD process.
Secondly, the invention improves the AD system by using NBs, and has the following technical advantages:
(1) promoting hydrolysis rate: NBs can increase the activity of hydrolases (e.g., alkaline phosphatase, acid phosphatase, alpha-glucosidase) to promote hydrolysis rate. In addition, there is a positive correlation between hydrolysis rate and water mobility, while NBs can interfere with the hydrogen bonding network of water, thereby increasing water mobility.
(2) Stability is enhanced: the NBs technology can relieve acid inhibition and ammonia inhibition in the AD system, so that the AD system is more stable. Such as Air-Nanobubble water (Air-NBW), can create a microaerophilic environment for the higher efficiency of electron transfer systems, thereby reducing volatile fatty acids by enhancing facultative bacterial activity, thereby alleviating acid inhibition; while having a higher fluidity and zeta potential (absolute value) to alleviate ammonia inhibition.
(3) Improving the methane yield: coenzyme F 420 Is an essential hydride carrier in the formation of hydrogenotrophic methane, and the activity of the carrier affects the yield of methane. Whereas NBs technology can increase coenzyme F 420 Thereby increasing methane production.
(4) The NBs technology has simple process, no chemical addition, low cost, environmental protection and high AD adaptation.
Third, the significant technological advances made by each of the main steps of the present invention are as follows:
step one: preparation method of nano bubble water
In this step, it was clarified that Air and CO were introduced separately by the nanobubble generator 2 、N 2 And He gas, and recirculating distilled water in the container to the generator circulation system for 20 minutes, can make the preparation of nano bubble water more standardized.
Step two: mixing the prepared nanometer bubble water of different types with cow dung at a ratio of 1:1, bottling, and sealing with a cover special for digestion reaction
In the step, the proportion of the mixed bottle is defined to be 1:1, and the mixed bottle is sealed by a cover special for digestion reaction, so that other air components can be prevented from entering a reaction system, and the accuracy and stability of the reaction are ensured.
Step three: selecting NBW type with good improving effect and economical and practical performance for carrying out AD process
In the step, a specific implementation scheme of selecting the NBW type with good improvement effect and economical and practical performance and then carrying out the AD process is provided, and the suitable NBW type can be selected according to actual conditions to carry out the AD process, so that the applicability and the practicability of the method are improved.
In summary, each step further defines implementation methods and operation steps, thereby improving stability, reliability and practicability of the method, and having higher technical progress.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of a method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung provided by an embodiment of the invention;
FIG. 2 is a diagram of a preparation mechanism of nano bubble water provided by the embodiment of the invention;
FIG. 3 is a diagram of an anaerobic digestion vessel- -anaerobic fermentation bottle provided by an embodiment of the present invention;
FIG. 4 is a graph of the change in pH, TA, TVFA/TA during anaerobic digestion at high and medium temperature provided by an embodiment of the invention;
FIG. 5 is a graph showing the change of SCOD, TAN, FAN in the anaerobic digestion process under the high and medium temperature conditions provided by the examples of the present invention;
FIG. 6 is a graph showing the cumulative methane yield and the daily methane production rate during anaerobic digestion at high and medium temperatures provided by the examples of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
In view of the problems of the prior art, the invention provides a method for enhancing anaerobic digestion, hydrolysis and methane production of cow dung, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the method for strengthening anaerobic digestion hydrolysis and methanogenesis of cow dung provided by the embodiment of the invention comprises the following steps:
s101, preparing nano bubble water;
s102, mixing nano bubble water with cow dung and bottling;
s103, selecting a proper NBW type and then carrying out an AD process.
As a preferred embodiment, the method for strengthening anaerobic digestion hydrolysis and methanogenesis of cow dung provided by the embodiment of the invention comprises the following steps:
step 1, a preparation method of nano bubble water comprises the following steps: air and CO are respectively introduced through a nano bubble generator 2 、N 2 And He gas, recirculating distilled water in the vessel to the generator circulation system for 20 minutes (as in fig. 2);
step 2, mixing the prepared nano bubble water of different types with cow dung in a ratio of 1:1, bottling, and sealing by a cover special for digestion reaction;
and step 3, selecting an NBW type with good improvement effect, and performing an AD process.
Further, the temperature passing through the nanobubble generator in step 1 was 20.+ -. 2 ℃.
Further, in step 1, during the preparation of NBW, the pressure is controlled to be 0.4MPa to 0.5MPa.
Further, the anaerobic fermentation bottles in the step 2 are respectively of the type of CO 2 -NBW、He-NBW、N 2 -NBW, air-NBW and placebo DW.
Further, in step 2, the digestion reaction special cover is provided with two small holes (with an inner diameter of 6 mm), a silica gel tube (with an inner diameter of 6 mm) connects one hole with the air bag for biogas sampling, and a peristaltic pump is used in the other hole to collect the digestion sample (as shown in fig. 3).
Further, in step 2, AD process was performed under conditions of high temperature (55.+ -. 1 ℃) and medium temperature (37.+ -. 1 ℃) respectively.
Further, in step 3, air-NBW type is selected.
In step 3, air-NBW and cow dung are respectively mixed and bottled in a mode of 1:1, 2:1 and 4:1, three corresponding blank control DW: CM=1:1, 2:1 and 4:1 are arranged, and AD processes are respectively carried out under the conditions of high temperature (55+/-1 ℃) and medium temperature (37+/-1 ℃).
The application embodiment of the invention provides an application of a method for strengthening anaerobic digestion hydrolysis and methanogenesis of cow dung in the anaerobic digestion hydrolysis and methanogenesis of cow dung.
Total Solids (TS), volatile Solids (VS), gas volumes, methane yields, trace elements (C, N, H, S), dissolved chemical oxygen demand (Soluble chemical oxygen demand, SCOD), pH, VFA, total ammonia nitrogen (Total ammonia nitrogen, TAN), free ammonia (Free ammonia nitrogen, FAN), total Alkalinity (TA) and microbial community analysis were used as test indicators for sampling on days 0, 5, 12, 22, 35, 50, respectively.
The pH value is an important index of the AD, and the pH value of each group of digestive juice in the AD process is stabilized between 8.06 and 8.45 under the high temperature condition; under the medium temperature condition, the pH value of each group of digestive juice is stabilized between 7.55 and 8. TA is closely related to buffering capacity and is provided by ammonia nitrogen formed by deamination of nitrogen-containing organic matters in an AD system, and TA of high Wen Zu is about 8958.55-11883.87 mg CaCO 3 L -1 The method comprises the steps of carrying out a first treatment on the surface of the TA of the mesophilic group is approximately 9672.06-12481.8 mg CaCO 3 L -1 (FIG. 4).
TVFA/TA is a good indicator of AD stability. TVFA/TA of each group on day 5 is respectively DW group 0.78 and CO under the high temperature condition 2 Group 0.38, N 2 Group 0.75, he group 0.62, air group 0.52; TVFA/TA of each group on day 5 is DW group 0.72 and CO respectively under medium temperature condition 2 Group 0.63, N 2 Set 0.83, he set 0.51, air set 0.34. The stability of the anaerobic digestion of Air group was shown to be best (fig. 4).
SCOD is an important index representing the dissolution of reducing substances by digests. SCOD on day 5 of each group under high temperature condition was 18112.99mg L for DW group respectively -1 、CO 2 Group 16978.49mg L -1 、N 2 Group 18179.76mg L -1 22178.28mg L of He group -1 Air group 24163.66mg L -1 The method comprises the steps of carrying out a first treatment on the surface of the SCOD of each group under medium temperature condition is 24640.86mg L of DW group respectively -1 、CO 2 Group 24829.94mg L -1 、N 2 Group 23922.34mg L -1 25132.48mg L of He group -1 Air group 25208.11mg L -1 (FIG. 5). The Air group has the most ideal effect of anaerobically digesting organic matters.
TAN of each group is 1219-1633.33 mg L under high temperature condition -1 Between each two groups of FANs are 353.04-419.47 mg L -1 Between them; TAN of each group is 1132.16-1606.66 mg L under medium temperature condition -1 Between each two groups of FANs are 50.62-167.62 mg L -1 In between (fig. 5), indicating that mesophilic methanogens are not affected by FAN.
The cumulative methane yield of each group in AD process under high temperature condition is 192.90mL g for DW group -1 VS added 、CO 2 Group 226.12mL g -1 VS added (17.22% more than DW group), N 2 Group 198.98mL g -1 VS added (3.15% increase compared to DW group), he group 184.74mL g -1 VS added 227.09mL g of Air group -1 VS added (and DW group)17.72% as compared to the previous; cumulative methane yields for each group at medium temperature were 148.05mL g for DW group, respectively -1 VS added 、CO 2 Group 151.20mL g -1 VS added (2.12% increase compared to DW group), N 2 Group 140.37mL g -1 VS added 154.85mL g of He group -1 VS added (4.59% increase compared to DW group), air group 162.39mL g -1 VS added (9.68% increase compared to DW group) (fig. 6). The Air group has the best effect of improving the AD process, and the change trend of the methane production rate of each group is basically consistent, namely, the methane production rate is increased firstly and then decreased, and finally the methane production rate tends to be gentle.
The following is a specific scheme of each embodiment provided by the invention:
1. the waste treatment scheme for the breeding industry comprises the following steps:
step one: the preparation method of the nano bubble water by using the nano bubble generator comprises the following specific steps: air and CO are respectively introduced 2 、N 2 And He gas, distilled water in the vessel was recycled to the generator circulation system for 20 minutes.
Step two: mixing the prepared nano bubble water with the aquaculture waste according to the proportion of 1:1, and placing the mixture into a container sealed by a special cover for digestion reaction.
Step three: and selecting a proper NBW type for carrying out an AD process, and particularly selecting the proper NBW type according to the characteristics of a processing object and actual requirements.
2. The urban sludge treatment scheme comprises the following steps:
step one: the preparation method of the nano bubble water by using the nano bubble generator comprises the following specific steps: air and CO are respectively introduced 2 、N 2 And He gas, distilled water in the vessel was recycled to the generator circulation system for 20 minutes.
Step two: mixing the prepared nano bubble water with municipal sludge according to a ratio of 1:1, and placing the mixture into a container sealed by a special cover for digestion reaction.
Step three: and selecting a proper NBW type for carrying out an AD process, and particularly selecting the proper NBW type according to the characteristics of a processing object and actual requirements.
3. The agricultural waste treatment scheme comprises the following steps:
step one: the preparation method of the nano bubble water by using the nano bubble generator comprises the following specific steps: air and CO are respectively introduced 2 、N 2 And He gas, distilled water in the vessel was recycled to the generator circulation system for 20 minutes.
Step two: mixing the prepared nano bubble water with agricultural waste according to the proportion of 1:1, and placing the mixture into a container sealed by a special cover for digestion reaction.
Step three: and selecting a proper NBW type for carrying out an AD process, and particularly selecting the proper NBW type according to the characteristics of a processing object and actual requirements.
4. The kitchen waste treatment scheme comprises the following steps:
step one: the preparation method of the nano bubble water by using the nano bubble generator comprises the following specific steps: air and CO are respectively introduced 2 、N 2 And He gas, distilled water in the vessel was recycled to the generator circulation system for 20 minutes.
Step two: mixing the prepared nano bubble water with kitchen waste according to the proportion of 1:1, and placing the mixture into a container sealed by a special cover for digestion reaction.
Step three: and selecting a proper NBW type for carrying out an AD process, and particularly selecting the proper NBW type according to the characteristics of a processing object and actual requirements.
5. The biomass energy treatment scheme comprises the following steps:
step one: the preparation method of the nano bubble water by using the nano bubble generator comprises the following specific steps: air and CO are respectively introduced 2 、N 2 And He gas, distilled water in the vessel was recycled to the generator circulation system for 20 minutes.
Step two: mixing the prepared nano bubble water and biomass energy according to the proportion of 1:1, and placing the mixture into a container sealed by a special cover for digestion reaction.
Step three: and selecting a proper NBW type for carrying out an AD process, and particularly selecting the proper NBW type according to the characteristics of a processing object and actual requirements.
6. The sewage treatment scheme comprises the following steps:
step one: the preparation method of the nano bubble water by using the nano bubble generator comprises the following specific steps: respectively are provided withIntroducing Air and CO 2 、N 2 And He gas, distilled water in the vessel was recycled to the generator circulation system for 20 minutes.
Step two: mixing the prepared nano bubble water with sewage according to the proportion of 1:1, and placing the mixture into a container sealed by a special cover for digestion reaction.
Step three: and selecting a proper NBW type for carrying out an AD process, and particularly selecting the proper NBW type according to the characteristics of a processing object and actual requirements.
The implementation of the specific schemes needs to pay attention to parameters such as control temperature, control pH value, control concentration of COD and the like of digestion reaction so as to ensure the effectiveness and high efficiency of AD reaction. Meanwhile, for different waste treatment objects, different NBW types are required to be selected according to actual conditions, so that the efficiency and gas production rate of the AD process are improved.
The foregoing is merely illustrative of specific embodiments of the present invention, and the scope of the invention is not limited thereto, but any modifications, equivalents, improvements and alternatives falling within the spirit and principles of the present invention will be apparent to those skilled in the art within the scope of the present invention.
Claims (10)
1. A method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung, comprising:
preparing nano bubble water;
mixing the nano bubble water with cow dung and bottling;
the AD procedure is performed by selecting the appropriate NBW type.
2. The method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung according to claim 1, wherein the method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung comprises the steps of:
step one, a preparation method of nano bubble water comprises the following steps: air and CO are respectively introduced through a nano bubble generator 2 、N 2 And He gas, recirculating distilled water in the vessel to the generator circulation system for 20 minutes;
step two, mixing the prepared nano bubble water of different types with cow dung in a ratio of 1:1, bottling, and sealing by a cover special for digestion reaction;
and thirdly, selecting an NBW type with better improvement effect, and performing an AD process.
3. The method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung according to claim 2, wherein the temperature of the nano bubble generator in the first step is 20+/-2 ℃;
in the first step, during the preparation of NBW, the pressure is controlled to be 0.4MPa to 0.5MPa.
4. The method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung according to claim 2, wherein the type of anaerobic fermentation bottle in the second step is CO 2 -NBW、He-NBW、N 2 -NBW, air-NBW and placebo DW.
5. The method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung according to claim 2, wherein in the second step, two small holes are formed on a cover special for digestion reaction, a silicone tube connects one hole with an air bag for biogas sampling, and a peristaltic pump is used for collecting digestion samples in the other hole.
6. The method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung according to claim 2, wherein in the second step, AD process is performed under the conditions of high temperature (55+ -1deg.C) and medium temperature (37+ -1deg.C), respectively.
7. The method for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung according to claim 2, wherein Air-NBW type is selected in step three.
8. The method for strengthening anaerobic digestion hydrolysis and methanogenesis of cow dung according to claim 2, wherein in the third step, air-NBW and cow dung are respectively subjected to 1:1, 2:1 and 4:1 mixing bottling, and corresponding three sets of blank control DW: CM=1:1, 2:1 and 4:1 are respectively subjected to AD process under the conditions of high temperature (55+/-1 ℃) and medium temperature (37+/-1 ℃).
9. A system for enhancing anaerobic digestion hydrolysis and methanogenesis of cow dung comprising:
the nanometer bubble water preparation module comprises: preparing nano bubble water through a nano bubble generator; the generator introduces Air and CO 2 、N 2 And He gas, and circulating distilled water in the container to a generator circulation system, and maintaining the circulation for 20 minutes, thereby preparing nano bubble water;
cow dung and nanometer bubble water mixing module: mixing the prepared nano bubble water with cow dung according to the proportion of 1:1, bottling and sealing, and sealing by using a cover special for digestion reaction;
NBW type selection module: selecting a proper NBW type to further strengthen the processes of anaerobic digestion hydrolysis and methanogenesis of cow dung according to the requirement; in this module, an appropriate NBW type may be selected according to factors such as improvement effect and economical practicality.
10. Use of a method of enhancing anaerobic digestion hydrolysis of cow dung and methanogenesis of any one of claims 1 to 8 for enhancing anaerobic digestion hydrolysis of cow dung and providing methane production.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310911069.9A CN116768439A (en) | 2023-07-24 | 2023-07-24 | Method and system for strengthening anaerobic digestion hydrolysis and methane production of cow dung |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310911069.9A CN116768439A (en) | 2023-07-24 | 2023-07-24 | Method and system for strengthening anaerobic digestion hydrolysis and methane production of cow dung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116768439A true CN116768439A (en) | 2023-09-19 |
Family
ID=87994668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310911069.9A Pending CN116768439A (en) | 2023-07-24 | 2023-07-24 | Method and system for strengthening anaerobic digestion hydrolysis and methane production of cow dung |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116768439A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305380A (en) * | 2023-10-08 | 2023-12-29 | 江苏省农业科学院 | Method for producing medium-chain carboxylic acid by utilizing nano bubble water with high curvature interface to enhance anaerobic fermentation of organic waste |
-
2023
- 2023-07-24 CN CN202310911069.9A patent/CN116768439A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305380A (en) * | 2023-10-08 | 2023-12-29 | 江苏省农业科学院 | Method for producing medium-chain carboxylic acid by utilizing nano bubble water with high curvature interface to enhance anaerobic fermentation of organic waste |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mohan et al. | Anaerobic biohydrogen production from dairy wastewater treatment in sequencing batch reactor (AnSBR): effect of organic loading rate | |
| Angelidaki et al. | Biomethanation and its potential | |
| Zhi et al. | How methane yield, crucial parameters and microbial communities respond to the stimulating effect of antibiotics during high solid anaerobic digestion | |
| Lin et al. | Methanogenic community dynamics in anaerobic co-digestion of fruit and vegetable waste and food waste | |
| Jiang et al. | Enhancing the performance of thermophilic anaerobic digestion of food waste by introducing a hybrid anaerobic membrane bioreactor | |
| Li et al. | Inhibitory effect of high phenol concentration in treating coal gasification wastewater in anaerobic biofilter | |
| Carrillo-Reyes et al. | Influence of added nutrients and substrate concentration in biohydrogen production from winery wastewaters coupled to methane production | |
| Tang et al. | Dry anaerobic digestion of ammoniated straw: Performance and microbial characteristics | |
| Tena et al. | Effects of several inocula on the biochemical hydrogen potential of sludge-vinasse co-digestion | |
| Takahashi et al. | Evaluation of treatment characteristics and sludge properties in a UASB reactor treating municipal sewage at ambient temperature | |
| Wang et al. | Nitrate addition improves hydrogen production from acidic fermentation of waste activated sludge | |
| CN116768439A (en) | Method and system for strengthening anaerobic digestion hydrolysis and methane production of cow dung | |
| Wanqin et al. | Effects of Fe2+ on the anaerobic digestion of chicken manure: a batch study | |
| Yu et al. | Multiple effects of trace elements on methanogenesis in a two-phase anaerobic membrane bioreactor treating starch wastewater | |
| Lee et al. | Feasibility test anaerobically enhancing methane yield under the injection of hydrogen and carbon dioxide | |
| CN117326683A (en) | Method for reinforcing low-temperature sewage co-metabolism treatment by recycling high-concentration organic wastewater | |
| Lin et al. | High-strength wastewater treatment using anaerobic processes | |
| Wu et al. | Profitable wastewater treatment by directly recovering organics for volatile fatty acids production | |
| WO2014007530A1 (en) | Method for promoting production of biogas using pancreatin in anaerobic digestion process | |
| Gu et al. | Characteristics of Biohydrogen Production and Performance of Hydrogen‐Producing Acetogen by Increasing Normal Molasses Wastewater Proportion in Anaerobic Baffled Reactor | |
| CN115181284B (en) | Fe-MOF/Ben @ CNTs composite conductive material, preparation method and application thereof | |
| KR20080025596A (en) | High rate methanc production system using anaerobic archaea | |
| CN107032576B (en) | Operation method for treating excess sludge by adopting horizontal anaerobic reactor | |
| CN113897318A (en) | Method for co-culturing protein degradation by using acetoacidophilic protein bacterium and sulfur reducing geobacillus | |
| CN112661374A (en) | Method for improving methane yield in sludge anaerobic digestion process by using micro-plastic |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |