CN116763796A - Preparation method and application of pheromone for cats and pheromone mixture for cats - Google Patents

Preparation method and application of pheromone for cats and pheromone mixture for cats Download PDF

Info

Publication number
CN116763796A
CN116763796A CN202310730316.5A CN202310730316A CN116763796A CN 116763796 A CN116763796 A CN 116763796A CN 202310730316 A CN202310730316 A CN 202310730316A CN 116763796 A CN116763796 A CN 116763796A
Authority
CN
China
Prior art keywords
pheromone
cat
acid
cats
methyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202310730316.5A
Other languages
Chinese (zh)
Inventor
徐牧云
张魏文
魏楚丰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mimi Chat Wuhan Biotechnology Co ltd
Original Assignee
Mimi Chat Wuhan Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mimi Chat Wuhan Biotechnology Co ltd filed Critical Mimi Chat Wuhan Biotechnology Co ltd
Priority to CN202310730316.5A priority Critical patent/CN116763796A/en
Publication of CN116763796A publication Critical patent/CN116763796A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/11—Aldehydes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
    • A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/575—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Abstract

The application provides a cat pheromone and a preparation method and application of a cat pheromone mixture, and the cat pheromone mixture comprise a plurality of functional combined pheromones, namely a cat pacifying type combined pheromone, a cat urine antagonistic type combined pheromone or a cat individual friendly type combined pheromone, wherein the cat pacifying type combined pheromone is a combination of a cat face pheromone and a cat pacifying pheromone, the cat urine antagonistic type combined pheromone is a combination of a cat face pheromone and a cat urine smell pheromone, and the cat individual friendly type combined pheromone is a combination of a cat face pheromone F4 and a cat pacifying pheromone. The application of the pheromone for cats is to prepare the pheromone mixed solution for cats into a pheromone patch. The pheromone combination and the application scheme provided by the application are closer to the marking and reading modes of cats under natural conditions, and the same function is regulated by adopting a plurality of pheromones, so that the pheromone combination and the application scheme have synergistic effect, the components are safe and effective, and the product is practical.

Description

Preparation method and application of pheromone for cats and pheromone mixture for cats
Technical Field
The application belongs to the technical field of pheromones, and particularly relates to a cat pheromone, a preparation method and application of a cat pheromone mixture.
Background
Pheromone (pheomone), also known as Pheromone, is a Semiochemical substance secreted by an individual into the body and perceived by other individuals of the same species through olfactory organs (e.g., the accessory olfactory bulb, the vomeropherin) to cause the latter to exhibit altered behavioral, emotional, psychological or physiological mechanisms.
Cats are one of the most common companion animals worldwide. Cats can deposit chemical irritants in the environment through glandular secretions, saliva, urine and faeces, which play a central role in the communication and social behavior of the cat.
In the prior art, researchers have found the following classes of pheromones on cats: 1. cat face pheromone (FFP, feline facial pheromones): is a chemical substance produced by the cheeks and glands surrounding the chin of a cat. Today, researchers have found five different facial pheromones on the cat's face (including chin, lips, tentacles, and cheeks), designated as F1 to F5. Wherein, F3 is the face pheromone mainly responsible for space orientation and mood stabilization, is used for marking objects and indicating that the objects are familiar and safe, and the use of F3 can increase the trust feeling of cats on strange places, thereby reducing the frequency of stress when the cats face the strange places. F4 is a pheromone released by the cat when rubbing other cats in the social environment, and is used for marking the rubbed individuals as familiar, so that the number of active attacks of the cat can be reduced. 2. Cat pacifying pheromone (CAP, cat Appeasing Pheromone): is a pheromone secreted by a kitten in lactation, and is used for pacifying and establishing a connection with a kitten. CAP may reduce social tension and conflicts in multi-cat households and may alleviate stress behavior of cats when new cats are introduced or environments are changed. 3. Cat urine odorant (CUO, cat Urine Odorants): is a pheromone in urine of male cats. Unlike the excessive marking of the male dogs after encountering their own urine, the cat does not perform urination and defecation after recognizing the urine pheromone, and no application of the cat urine odorant is available. 4. Inter-cat digital pheromone (FIS, feline Interdigital Semiochemical): when the cat is scratched, the pheromone secreted from the gland on the foot pad of the paw to the surface of the object to be scratched is used for guiding the cat to scratch on the correct surface.
Instead of relying on natural production of the cat, researchers wipe the part of the cat secreting the pheromone with an organic solvent, analyze the main components of the pheromone by gas chromatography-mass spectrometry (GC-MS), and then perform artificial synthesis by using a chemical method. In 1995, pageat P (Ceva, france) proposed the first artificial synthesis and application of the cat pheromone in European patent EP 0724832B1, and more than 20 experiments have been carried out for 28 years so far to verify the regulation of the cat behavior by the artificial synthesis pheromone. These synthetic pheromone products are often presented in the form of sprays or plug-in diffusers, such as the Feliway series of Ceva, france, the Shanghai Ai Bai organism's small shell Fei Leshu series, china.
The design focus of these products has been on the large scale volatilization and diffusion of the synthesized pheromones. However, as people have become increasingly aware of pheromones, the structural properties of pheromones appear to be heterogeneous from volatile small molecules to non-volatile peptides. Volatilization is not the only way that pheromones function. Cat follows in daily pheromone marking behavior: generating a partial visual stimulus- & gtfriction marking of the environment/individual- & gtapproaching a mode of sniffing the detection pheromone read information. The prior product such as a plug-in diffuser has larger limitation in use, such as hidden danger of electricity consumption, lack of visual stimulus, insufficient portability and the like, and the natural behavior mode of the cat is that marked pheromones are found at different places in the environment to generate behavior change instead of filling the whole house with the pheromones; the spray product needs to be sprayed for 8-15 times when being used once, wherein more than 80% of the spray product is volatile solvent ethanol of the pheromone, and only contains 10% -20% of the pheromone. After each spraying, the alcohol which can stimulate the respiratory tract mucosa of the cat needs 5-10 minutes to volatilize, so that the cat can smell closely to play the role of the pheromone. Has the defects of difficult control of accurate dosage, lack of visual stimulus, difficult carrying, long waiting period and the like. Aiming at the problems, the application aims to provide a mode for using the pheromone as the pheromone patch, which provides accurate dosage for single-use of the pheromone, and simultaneously provides visual stimulus for the cat by using the patch characteristic.
Meanwhile, most of the existing synthetic artificial pheromone products are single pheromones, such as Feliway classical series of Ceva and small shell Fei Leshu series, namely, cat face pheromone F3 is matched with different solvents for diffusion; feliway friends series, namely cat pacifying pheromone CAP, are diffused by matching with different solvents. However, in the application field of pheromones, such as pest control, better effect can be achieved when a plurality of pheromones are used cooperatively. In view of such problems, the present application is intended to provide a way of using a plurality of pheromones in combination.
Disclosure of Invention
Aiming at the problems existing in the prior art, the application adopts the following technical scheme for solving the problems existing in the prior art:
the cat pheromone comprises a plurality of functional combined pheromones, namely a cat pacifying combined pheromone, a cat urine antagonistic combined pheromone or a cat individual friendly combined pheromone, wherein the cat pacifying combined pheromone is a combination of a cat face pheromone F3 and a cat pacifying pheromone CAP, the cat urine antagonistic combined pheromone is a combination of a cat face pheromone F3 and a cat urine odorant CUO, and the cat individual friendly combined pheromone is a combination of a cat face pheromone F4 and a cat pacifying pheromone CAP.
The pacifying type combined pheromone for the cat comprises the following chemical components in percentage by mass: 20-60% oleic acid, 15-24% palmitic acid, 10-20% azelaic acid, 10-15% pimelic acid, 1-5% linoleic acid, 1-5% valeric acid, 0.5-2% lauric acid, 0.5-2% myristic acid, 1-5% stearic acid, 1-2% lactic acid.
Further, each fatty acid in the pacifying type combined pheromone for cats can be replaced by an equivalent amount of corresponding esterified compounds, wherein the corresponding esterified compounds are respectively as follows: methyl oleate, methyl palmitate, dimethyl azelate, dimethyl pimelate, methyl linoleate, methyl valerate, methyl laurate, methyl myristate, methyl stearate, and methyl lactate.
Further, the pacifying type composition pheromone for cats preferably contains 40% of oleic acid, 20% of palmitic acid, 17% of pimelic acid, 15% of azelaic acid, 1% of lauric acid, 1.8% of myristic acid, 2% of stearic acid, 1.2% of linoleic acid, 1% of lactic acid and 1% of valeric acid.
The urine antagonistic type combined pheromone for cats comprises the following chemical components in percentage by mass: 26-60% oleic acid, 15-24% palmitic acid, 10-20% azelaic acid, 10-20% pimelic acid, 1-2% isopentenyl aldehyde (CAS: 107-86-8), 1-2% 3-methyl-3-buten-1-ol (CAS: 763-32-6), 1-2% isopentenyl alcohol (CAS: 556-82-1), 1-2% formic acid-3-mercapto-3-methylbutyl ester (CAS: 50746-10-6), 1-2% 3-methoxy-3-methylbutanol (CAS: 34300-94-2).
Further, each fatty acid in the urine antagonistic combination pheromone for cats can be replaced by an equivalent amount of corresponding esterified compounds, wherein the corresponding esterified compounds are respectively as follows: methyl oleate, methyl palmitate, dimethyl azelate, dimethyl pimelate.
Further, the urine type combined pheromone for cats preferably comprises the following components: 40% oleic acid, 20% palmitic acid, 17% azelaic acid, 15% pimelic acid, 2% isopentenyl aldehyde (CAS: 107-86-8), 2% 3-methyl-3-buten-1-ol (CAS: 763-32-6), 2% isopentenol (CAS: 556-82-1), 1% formic acid-3-mercapto-3-methylbutyl ester (CAS: 50746-10-6), 1% 3-methoxy-3-methylbutanol (CAS: 34300-94-2).
The individual friendly combined pheromone for cats comprises the following chemical components in percentage by mass: 1-10% of a stanol (CAS: 360-68-9), 12-60% of oleic acid, 15-24% of palmitic acid, 10-20% of pimelic acid, 10-20% of azelaic acid, 1-5% of linoleic acid, 1-5% of valeric acid, 0.5-2% of lauric acid, 0.5-2% of myristic acid and 1-5% of stearic acid.
Further, each fatty acid in the individual friendly type combined pheromone for cats can be replaced by an equivalent amount of corresponding esterified compounds, wherein the corresponding esterified compounds are respectively as follows: methyl oleate, methyl palmitate, dimethyl pimelate, dimethyl azelate, methyl n-butyrate, methyl linoleate, methyl valerate, methyl laurate, methyl myristate, methyl stearate.
Further, the individual friendly type combination pheromone for cats preferably contains 3% of stigmastanol, 40% of oleic acid, 18% of palmitic acid, 17% of pimelic acid, 15% of azelaic acid, 1% of lauric acid, 1% of myristic acid, 2% of stearic acid, 2% of linoleic acid, and 1% of valeric acid.
The pheromone for cats is prepared into a pheromone mixture for cats, and the preparation method of the pheromone mixture for cats comprises the following steps:
step 1, preparing a combined pheromone with required functions: mixing the corresponding raw materials according to a proportion, selecting an organic solvent which is harmless to people and pets and has small skin irritation, such as ethanol, propylene glycol and ethyl acetate, wherein the proportion of the combined pheromone is 10-30%, the proportion of the solvent is 70-90%, sequentially adding samples, fully stirring and uniformly mixing, and dissolving for 5min at the temperature of 40 ℃ in a constant-temperature water bath to prepare light yellow oily mixed solution with the density of 0.73-0.91 g/ml;
step 2, adding a euphoria with the content of 0.1% of the total amount of the prepared combined pheromone into the mixed solution obtained in the step 1, wherein the selected euphoria is from plants such as catnip, valerian, polygonum multiflorum, lonicera japonica and the like; the gladness agent comprises one or more of cis-trans nepetalactone (CAS: 21651-62-7), trans-cis nepetalactone, dihydronepetalactone, isodihydronepetalactone, neonepetalactone, actinidine, argentine or isoArgentine, and the chemical structure of the gladness agent is as follows:
step 3, adding an antiseptic and antioxidant into the mixed solution obtained in the step 2 to obtain a pheromone mixture for cats: selecting fat-soluble preservative harmless to human and pets, such as BHA, BHT, vitamin E, herba Rosmarini officinalis extract, etc., wherein the addition amount of the preservative and antioxidant is 0.1% of the total amount of the mixed solution prepared in step 2;
step 4, checking the temperature stability of the pheromone mixture for cats obtained in the step 3: and (3) respectively placing the prepared liquid in a refrigerator at the temperature of minus 20 ℃ and a water bath kettle at the temperature of 50 ℃, standing and storing for 28 days, checking the liquid, taking out a sample every 7 days, naturally recovering to room temperature, and checking the color, smell and transparency, wherein no coagulation and no crystal precipitation are caused.
The application of the pheromone for cats is that the pheromone mixed liquid for cats is prepared into an pheromone patch, the pheromone patch sequentially comprises an adhesive layer 1, an isolating layer 2, an pheromone storage layer A3 and an pheromone storage layer B4, the pheromone storage layer is uniformly coated with the prepared pheromone mixture for cats, the pheromone storage layer is of a double-layer structure, fatty acids with different volatilities can be guaranteed to volatilize uniformly, and the pheromone storage layer A3 and the pheromone storage layer B4 are made of spunlaced cloth, non-woven fabrics or PU materials; the isolating layer 2 is a Polyethylene (PE) laminated film and is used for separating the pheromone storage layer from the adhesive layer.
The pheromone is stuck with a pattern which can be printed to attract the cat, the visible colors of the cat eye and human eyes are different, and the red color with the warning effect for the human is not in the visible light range of the cat eye; the cat eye has a tapetum, which is highly reflective and has reduced yellow saturation. Preferably, white, blue, black, green are printed as the main tone of the pattern of the pheromone storage layer. Furthermore, the pheromone prepared by the application can be used as a paste, gel and other mediums, so that the consumption of flammable solvents is reduced, and the waiting time of pets is shortened.
The application has the following advantages:
1. provided are a combination pheromone: different from the prior art that one function is regulated by only one pheromone, the pheromone combination and application scheme provided by the application are closer to the marking and reading modes of cats under natural conditions, and the combination and application scheme has a synergistic effect by adopting a plurality of pheromones for regulating the same function. In the improvement of the prior art, it is found that the pheromone, namely isopentenyl aldehyde (CAS: 107-86-8), 3-methyl-3-buten-1-ol (CAS: 763-32-6), isopentenol (CAS: 556-82-1), formic acid-3-mercapto-3-methylbutyl ester (CAS: 50746-10-6), 3-methoxy-3-methylbutanol (CAS: 34300-94-2) and the like, in the urine of the cat are added in the urine disorder antagonism function, so that the effect on the urine disorder antagonism of the cat is more remarkable by using a single pheromone as a control group. In an individual friendly function, the addition of the stigmastanol significantly reduces the stress of the cat in the face of strangers. Further, since the facial secretions of pheromones are known to vary with the sex, age, species and individual physiological condition, emotional condition and grade status of each cat, the content of each component of each pheromone of the cat analyzed by gas chromatography-mass spectrometry (GC-MS) is a range, and it is difficult to form accurate guidance for application. Therefore, design-Expert software is used for carrying out Response Surface Method (RSM) test Design and analysis, and the RSM is an optimization method for comprehensive test Design and mathematical modeling, so that the test times can be effectively reduced, and interaction among influencing factors can be inspected. The optimal experimental design and the results of each functional combination pheromone are shown in the attached experiment 1.
2. High content pheromones are provided: the pheromone proportion in the formula of the application is higher, and the effect and durability of the product are improved.
3. The accurate consumption of pheromone is controlled: the single use amount is accurate, repeated spraying is not needed, and the environment is protected and saved.
4. Adding a trace amount of a gladness agent: and a trace of gladness agent is added to help the cat to open the nose plow and keep the emotion happy.
5. The ethanol content is lower: compared with the traditional pheromone product, the ethanol content in the application is lower, and the potential safety hazard is reduced.
6. The convenient use mode is as follows: the user only needs to uncover the pheromone paster and paste the pheromone paster around the place where the cat is on or off, and volatilizes for 1-2 minutes to produce an effect.
7. Providing visual stimuli: the patch has pattern printing, provides visual stimulus for the cat, and is more close to the actual scene of the production and reading pheromone of the cat.
Drawings
FIG. 1 is a schematic diagram of a split structure of each layer of an pheromone patch of the present application;
FIG. 2 is a schematic diagram of the overall structure of the pheromone patch of the present application;
wherein: 1-adhesive layer, 2-isolating layer, 3-pheromone storage layer A, 4-pheromone storage layer B.
Detailed Description
The technical scheme of the application is further specifically described below through examples and with reference to the accompanying drawings. Experiment one: preferably single use dosage of pheromone
Table 1 experiment one pheromone component proportion experiment
The plow nose reaction number (/ 20) represents: the placebo and each experimental group of agents were applied to a pheromone patch and sniffed by 20 cats. The number of plow nose device reactions that occurred was recorded. ( Plow nose reaction: the physiological response of a non-primate mammal after detecting the pheromone is manifested by the animal rolling the upper lip backwards, exposing the incisors and gums, then inhaling and maintaining this posture for a few seconds )
The contact-to-mole ratio (/ 20) represents: the placebo and each experimental group of agents were applied to a pheromone patch and sniffed by 20 cats. The cat smells the medium stained with the test agent and then rubs the medium or other places with the head, face.
Average first-meal time (min) for unfamiliar environment: placing the cat into a new environment, slowly placing the food bowl after slowly walking without looking at eyes of the experimental cat, and after the food bowl is returned to the outside of the cat cage by 1 meter, observing feeding behaviors of the cat, and recording first feeding time of the cat.
Experiment one experiment result: based on the data in table 1, the effects of each component on the cat on the plow nasal response were similar (no significant difference), while group 4 was more significant on the reduction of the feeding time in the strange environment (P < 0.01).
Experiment II: pheromone patch and stock solution use durability comparison experiment
TABLE 2 experiments of pheromones for cats on different application media
Experiment two implementation steps:
the experimental materials are respectively placed in a normal temperature environment (25.6 ℃) to volatilize for a designated time; the test material is smelled by the cat, the occurrence of the plow nose device reaction is marked as positive, the no plow nose device reaction is marked as negative, and the test cat with 80 percent of the positive is marked as effective.
Experimental results of experiment two:
the glass medium sprayed with the pacifying type combined pheromone stock solution is negative after volatilizing for 2 hours, the spunlaced cloth is negative after volatilizing for 6 hours, and the PU is negative after volatilizing for 16 hours. The pheromone stock solution is produced in a patch form, so that the pheromone retention time can be effectively prolonged.
Experiment III: comparison experiment of monomer pheromone and combined pheromone
Table 3 three groups of experimental reagents for experiment three:
experiment three implementation steps:
10 house cats (more than 10 cats, including both sterile and non-sterile cats) with long term urine-disordered habit cats were invited to participate in the experiment. Synchronously spraying the reagent of an experimental group, a control group and a blank group at three different places of the messy urine of the cat in the same cat house once every 4 hours for the first 7 days; 7-14d, spraying every 8 hours, 14-21d, spraying every 24 hours, and continuously using for 21 days. The telephone return visit counts the number of urine occurrences at three sites fed back by the cat house owners after the reagents are used.
Experimental results of experiment three:
table 4 experiment results of experiment three
Conclusion of experiment three: compared with single pheromone and absolute ethyl alcohol, the urine antagonistic combined pheromone obviously reduces the frequency of messy urine of cats in a multi-cat family pressure environment.
The protective scope of the application is not limited to the embodiments described above, but it will be apparent to those skilled in the art that various modifications and variations can be made in the present application without departing from the scope and spirit of the application. It is intended that the present application also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (10)

1. A pheromone for cats, which is characterized in that: the cat-used urine antagonistic type combined pheromone comprises a plurality of functional combined pheromones, namely a cat-used pacifying type combined pheromone, a cat-used urine antagonistic type combined pheromone or a cat-used individual friendly type combined pheromone, wherein the cat-used pacifying type combined pheromone is a combination of a cat face pheromone and a cat pacifying pheromone, the cat-used urine antagonistic type combined pheromone is a combination of a cat face pheromone and a cat urine odorant, and the cat-used individual friendly type combined pheromone is a combination of a cat face pheromone and a cat pacifying pheromone.
2. A cat pheromone as claimed in claim 1, wherein: the pacifying type combined pheromone for the cat comprises the following chemical components in percentage by mass: 20-60% oleic acid, 15-24% palmitic acid, 10-20% azelaic acid, 10-15% pimelic acid, 1-5% linoleic acid, 1-5% valeric acid, 0.5-2% lauric acid, 0.5-2% myristic acid, 1-5% stearic acid, 1-2% lactic acid.
3. A cat pheromone as claimed in claim 2, wherein: the fatty acids in the cat pacifying type combined pheromone can be replaced by equivalent corresponding esterified compounds, wherein the corresponding esterified compounds are respectively as follows: methyl oleate, methyl palmitate, dimethyl azelate, dimethyl pimelate, methyl linoleate, methyl valerate, methyl laurate, methyl myristate, methyl stearate, and methyl lactate.
4. A cat pheromone as claimed in claim 1, wherein: the urine antagonistic type combined pheromone for cats comprises the following chemical components in percentage by mass: 26-60% oleic acid, 15-24% palmitic acid, 10-20% azelaic acid, 10-20% pimelic acid, 1-2% isopentenyl aldehyde (CAS: 107-86-8), 1-2% 3-methyl-3-buten-1-ol (CAS: 763-32-6), 1-2% isopentenyl alcohol (CAS: 556-82-1), 1-2% formic acid-3-mercapto-3-methylbutyl ester (CAS: 50746-10-6), 1-2% 3-methoxy-3-methylbutanol (CAS: 34300-94-2).
5. A cat pheromone as in claim 4 wherein: each fatty acid in the urine antagonistic combined pheromone for cats can be replaced by an equivalent amount of corresponding esterified compounds, and the corresponding esterified compounds are respectively as follows: methyl oleate, methyl palmitate, dimethyl azelate, dimethyl pimelate.
6. A cat pheromone as claimed in claim 1, wherein: the individual friendly combined pheromone for cats comprises the following chemical components in percentage by mass: 1-10% of a stanol (CAS: 360-68-9), 12-60% of oleic acid, 15-24% of palmitic acid, 10-20% of pimelic acid, 10-20% of azelaic acid, 1-5% of linoleic acid, 1-5% of valeric acid, 0.5-2% of lauric acid, 0.5-2% of myristic acid and 1-5% of stearic acid.
7. A cat pheromone as in claim 6, wherein: each fatty acid in the individual friendly combination pheromone for cats can be replaced by an equivalent amount of corresponding esterified compounds, wherein the corresponding esterified compounds are respectively as follows: methyl oleate, methyl palmitate, dimethyl pimelate, dimethyl azelate, methyl n-butyrate, methyl linoleate, methyl valerate, methyl laurate, methyl myristate, methyl stearate.
8. A feline pheromone as claimed in any one of claims 1 to 7, prepared as a feline pheromone mixture, characterized in that: the preparation method of the pheromone mixture for cats comprises the following steps:
step 1, preparing a combined pheromone with required functions: mixing the corresponding raw materials according to a proportion, selecting an organic solvent which is harmless to people and pets and has small skin irritation, such as ethanol, propylene glycol and ethyl acetate, wherein the proportion of the combined pheromone is 10-30%, the proportion of the solvent is 70-90%, sequentially adding samples, fully stirring and uniformly mixing, and dissolving for 5min at the temperature of 40 ℃ in a constant-temperature water bath to prepare light yellow oily mixed solution with the density of 0.73-0.91 g/ml;
step 2, adding a euphoria with the content of 0.1% of the total amount of the prepared combined pheromone into the mixed solution obtained in the step 1, wherein the selected euphoria is from plants such as catnip, valerian, polygonum multiflorum, lonicera japonica and the like; the gladness agent comprises one or more of cis-trans nepetalactone (CAS: 21651-62-7), trans-cis nepetalactone, dihydronepetalactone, isodihydronepetalactone, neonepetalactone, actinidine, argentine or isoArgentine, and the chemical structure of the gladness agent is as follows:
step 3, adding an antiseptic and antioxidant into the mixed solution obtained in the step 2 to obtain a pheromone mixture for cats: selecting fat-soluble preservative harmless to human and pets, such as BHA, BHT, vitamin E, herba Rosmarini officinalis extract, etc., wherein the addition amount of the preservative and antioxidant is 0.1% of the total amount of the mixed solution prepared in step 2;
step 4, checking the temperature stability of the pheromone mixture for cats obtained in the step 3: and (3) respectively placing the prepared liquid in a refrigerator at the temperature of minus 20 ℃ and a water bath kettle at the temperature of 50 ℃, standing and storing for 28 days, checking the liquid, taking out a sample every 7 days, naturally recovering to room temperature, and checking the color, smell and transparency, wherein no coagulation and no crystal precipitation are caused.
9. The use of a pheromone for cats according to any one of claims 1 to 7 for preparing a pheromone patch from a mixed solution of pheromones for cats, wherein: the pheromone patch sequentially comprises an adhesive layer, an isolation layer, a pheromone storage layer A and a pheromone storage layer B, wherein the pheromone storage layer is uniformly coated with the prepared mixture of the pheromones for cats, and the pheromone patch is of a double-layer structure.
10. Use of a cat pheromone as claimed in claim 9, wherein: the pheromone storage layer A and the pheromone storage layer B are made of spunlaced cloth, non-woven cloth or PU materials; the isolation layer 2 is a polyethylene laminated film.
CN202310730316.5A 2023-06-20 2023-06-20 Preparation method and application of pheromone for cats and pheromone mixture for cats Pending CN116763796A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310730316.5A CN116763796A (en) 2023-06-20 2023-06-20 Preparation method and application of pheromone for cats and pheromone mixture for cats

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310730316.5A CN116763796A (en) 2023-06-20 2023-06-20 Preparation method and application of pheromone for cats and pheromone mixture for cats

Publications (1)

Publication Number Publication Date
CN116763796A true CN116763796A (en) 2023-09-19

Family

ID=88005828

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310730316.5A Pending CN116763796A (en) 2023-06-20 2023-06-20 Preparation method and application of pheromone for cats and pheromone mixture for cats

Country Status (1)

Country Link
CN (1) CN116763796A (en)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5709863A (en) * 1995-02-03 1998-01-20 Pageat; Patrick Properties of cats' facial pheromones
RU2360406C1 (en) * 2008-02-15 2009-07-10 Леонид Геннадьевич Менчиков Preparation for control over territorial marks of domestic cat
CN106456590A (en) * 2014-03-18 2017-02-22 信息化学与应用动物行为学研究院 Cat appeasing pheromone
US20170348267A1 (en) * 2014-12-24 2017-12-07 Virbac Soothing Composition for Animals, Comprising at Least One Fatty Acid and Nepetalactone
US20180169053A1 (en) * 2014-03-18 2018-06-21 Institut De Recherche Ensemiochimie Et Ethologie Appliquee Semiochemical compositions to reduce social conflicts with cats
CN110917241A (en) * 2019-12-13 2020-03-27 苏州弗艾柏生物科技有限公司 Cat appeasing pheromone and application thereof
CN112516128A (en) * 2020-12-08 2021-03-19 上海弗艾柏生物科技有限公司 Cat face pheromone and application thereof
CN116036066A (en) * 2022-12-28 2023-05-02 华农（肇庆）生物产业技术研究院有限公司 Cat face pheromone composition and preparation method and application thereof
US20230157281A1 (en) * 2020-03-24 2023-05-25 Texas Tech University System Compositions and methods for repelling animals from an object

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5709863A (en) * 1995-02-03 1998-01-20 Pageat; Patrick Properties of cats' facial pheromones
RU2360406C1 (en) * 2008-02-15 2009-07-10 Леонид Геннадьевич Менчиков Preparation for control over territorial marks of domestic cat
CN106456590A (en) * 2014-03-18 2017-02-22 信息化学与应用动物行为学研究院 Cat appeasing pheromone
US20180169053A1 (en) * 2014-03-18 2018-06-21 Institut De Recherche Ensemiochimie Et Ethologie Appliquee Semiochemical compositions to reduce social conflicts with cats
CN114732810A (en) * 2014-03-18 2022-07-12 信息化学与应用动物行为学研究院 Cat appeasing pheromone
US20170348267A1 (en) * 2014-12-24 2017-12-07 Virbac Soothing Composition for Animals, Comprising at Least One Fatty Acid and Nepetalactone
CN110917241A (en) * 2019-12-13 2020-03-27 苏州弗艾柏生物科技有限公司 Cat appeasing pheromone and application thereof
US20230157281A1 (en) * 2020-03-24 2023-05-25 Texas Tech University System Compositions and methods for repelling animals from an object
CN112516128A (en) * 2020-12-08 2021-03-19 上海弗艾柏生物科技有限公司 Cat face pheromone and application thereof
CN116036066A (en) * 2022-12-28 2023-05-02 华农（肇庆）生物产业技术研究院有限公司 Cat face pheromone composition and preparation method and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHRISTIAN STARKENMANN ET AL: "Odorant volatile sulfur compounds in caturine: occurrence of (+/-)-3, 7-dimethyloct-3-sulfanyl-6-en-1-ol and its cysteine conjugate precursor", 《FLAVOUR FRAGR. J》, vol. 30, 6 October 2014 (2014-10-06), pages 91 *
XAVIER DE JAEGER ET AL.，: "An Initial Open-Label Study of a Novel Pheromone Complex for Use in Cats", 《VETERINARY MEDICINE》, 20 March 2021 (2021-03-20), pages 105 - 116 *
代 丽: "特别栏目", 《科技导报》, vol. 26, no. 12, 31 December 2008 (2008-12-31), pages 13 *

Similar Documents

Publication Publication Date Title
Stoddart The scented ape: the biology and culture of human odour
Association of Food et al. Appraisal of the safety of chemicals in foods, drugs, and cosmetics
US5709863A (en) Properties of cats&#39; facial pheromones
Pageat et al. Current research in canine and feline pheromones
Veissier et al. Ontogeny of social awareness in domestic herbivores
Johnston Chemical communication in golden hamsters: From behavior to molecules and neural mechanisms.
Crews Effects of different components of male courtship behaviour on environmentally induced ovarian recrudescence and mating preferences in the lizard, Anolis carolinensis
US8933126B2 (en) Methods of administering a mixture of fatty acids for the treatment of non-human mammals
Terlouw et al. Behavioural responses of cattle to the odours of blood and urine from conspecifics and to the odour of faeces from carnivores
CN110917241A (en) Cat appeasing pheromone and application thereof
Stanton et al. Thyrotropin-releasing hormone effects in the central nervous system: dependence on arousal state
Van Honk et al. A sex pheromone from the mandibular glands in bumblebee queens
Brouette-Lahlou et al. Is rat-dam licking behavior regulated by pups’ preputial gland secretion?
CN110179697A (en) A kind of plants essential oil micro emulsion repellant and its preparation method and application
Roberts et al. Evolutionary psychology and perfume design
CN116763796A (en) Preparation method and application of pheromone for cats and pheromone mixture for cats
CN116036066B (en) Cat face pheromone composition and preparation method and application thereof
Lee et al. Social chemosignals in five Belontiidae (Pisces) species.
Zuri et al. Conspecific odor investigation by gray short-tailed opossums (Monodelphis domestica)
Gawienowski et al. Androgen dependence of a marking pheromone in rat urine
Melo et al. Communication by olfactory signals in rabbits: its role in reproduction
Stoddart The role of olfaction in the evolution of human sexual biology: An hypothesis
Burgess et al. Maintenance of the crab louse, Pthirus pubis, in the laboratory and behavioural studies using volunteers
Lee et al. Salivary cues in the mouse: a preliminary study
Garstka et al. Pheromones and reproduction in garter snakes

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination