CN116754764B - Fluorescent immunochromatography rapid quantitative detection device and application method thereof - Google Patents
Fluorescent immunochromatography rapid quantitative detection device and application method thereof Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 238000003317 immunochromatography Methods 0.000 title claims abstract description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 110
- 230000007246 mechanism Effects 0.000 claims abstract description 75
- 230000008569 process Effects 0.000 claims abstract description 26
- 238000013016 damping Methods 0.000 claims description 67
- 230000000670 limiting effect Effects 0.000 claims description 50
- 230000007306 turnover Effects 0.000 claims description 28
- 238000005520 cutting process Methods 0.000 claims description 17
- 238000004804 winding Methods 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims 2
- 230000001105 regulatory effect Effects 0.000 description 11
- 230000033228 biological regulation Effects 0.000 description 10
- 238000005457 optimization Methods 0.000 description 7
- 230000009471 action Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
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- 230000006978 adaptation Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000003746 surface roughness Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/02—Devices for feeding articles or materials to conveyors
- B65G47/04—Devices for feeding articles or materials to conveyors for feeding articles
- B65G47/12—Devices for feeding articles or materials to conveyors for feeding articles from disorderly-arranged article piles or from loose assemblages of articles
- B65G47/14—Devices for feeding articles or materials to conveyors for feeding articles from disorderly-arranged article piles or from loose assemblages of articles arranging or orientating the articles by mechanical or pneumatic means during feeding
- B65G47/1407—Devices for feeding articles or materials to conveyors for feeding articles from disorderly-arranged article piles or from loose assemblages of articles arranging or orientating the articles by mechanical or pneumatic means during feeding the articles being fed from a container, e.g. a bowl
- B65G47/1414—Devices for feeding articles or materials to conveyors for feeding articles from disorderly-arranged article piles or from loose assemblages of articles arranging or orientating the articles by mechanical or pneumatic means during feeding the articles being fed from a container, e.g. a bowl by means of movement of at least the whole wall of the container
- B65G47/1421—Vibratory movement
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/22—Devices influencing the relative position or the attitude of articles during transit by conveyors
- B65G47/24—Devices influencing the relative position or the attitude of articles during transit by conveyors orientating the articles
- B65G47/248—Devices influencing the relative position or the attitude of articles during transit by conveyors orientating the articles by turning over or inverting them
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G2201/00—Indexing codes relating to handling devices, e.g. conveyors, characterised by the type of product or load being conveyed or handled
- B65G2201/02—Articles
- B65G2201/0235—Containers
- B65G2201/025—Boxes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application relates to the technical field of immunochromatography detection, and discloses a fluorescent immunochromatography rapid quantitative detection device and a use method thereof. The rapid quantitative detection device comprises a vibration disc type feeding machine, a sample detection machine, a belt conveyor arranged between the vibration disc type feeding machine and the sample detection machine, a data acquisition mechanism arranged on the belt conveyor and an adjusting mechanism arranged below the belt conveyor, wherein the adjusting mechanism comprises an electric push rod, a reagent box overturning mechanism and an overturning auxiliary mechanism; according to the application, the adjusting mechanism is arranged on the belt conveyor, when the corresponding reagent box needs to be overturned, the reagent box overturning mechanism is pushed to move towards the reagent box to be overturned by the electric push rod and is matched with the overturning auxiliary mechanism, so that the reagent box can be overturned for one hundred and eighty degrees, and the whole process only needs one power source.
Description
Technical Field
The application belongs to the technical field of immunochromatography detection, and particularly relates to a fluorescent immunochromatography rapid quantitative detection device and a use method thereof.
Background
The fluorescent immunochromatography technology is a novel membrane detection technology based on antigen-antibody specific immunoreaction, the technology uses strip fiber chromatographic materials fixed with detection lines and quality control lines as stationary phases, test liquid as mobile phases, fluorescent labeled antibodies or antigens are fixed on a connecting pad, and analytes are enabled to move on chromatographic strips through capillary action.
The fluorescent immunochromatography analyzer is an instrument for automatically judging the concentration of a sample negative positive or a sample to be detected in a detection card, the fluorescent immunochromatography analyzer used in the market at present is various, but is mainly used for realizing the effect of automatic high-efficiency detection, and besides the sample collection process, the automatic processing of the processes such as feeding, conveying and inputting the sample into the reagent box is realized, wherein the automatic feeding and conveying process of the reagent box mainly adopts a vibrating disc type feeding machine and is matched with equipment such as a conveying belt to automatically arrange and orderly convey the reagent box into the sample detection machine for sample detection, but in the feeding and conveying process, the reagent box is easy to have the condition of reversing the front side and the back side, the reagent box needs to be overturned and adjusted, and the conventional overturning mechanism mainly adopts a plurality of power source equipment control clamps for adjustment, but because of the arrangement of a plurality of power sources, the reagent box is large in size and cannot be suitable for a conveyor for conveying small-sized articles such as the reagent box and consumes more energy.
Disclosure of Invention
The application aims to solve the problems and provide a fluorescent immunochromatography rapid quantitative detection device and a use method thereof.
The application realizes the above purpose through the following technical scheme:
the rapid quantitative detection device comprises a vibration disc type feeding machine, a sample detection machine, a belt conveyor arranged between the vibration disc type feeding machine and the sample detection machine, a data acquisition mechanism arranged on the belt conveyor and an adjusting mechanism arranged below the belt conveyor, wherein the vibration disc type feeding machine is used for conveying a kit onto the belt conveyor, and the belt conveyor is used for conveying the kit into the sample detection machine;
the data acquisition mechanism comprises a first range finder, a second range finder and a counter which are sequentially arranged on the belt conveyor, wherein the first range finder and the second range finder are both used for acquiring the space data of the kit;
the adjusting mechanism comprises a control box arranged below the belt conveyor, two electric push rods symmetrically connected to the upper end of the control box, a reagent box turnover mechanism connected to the output end of the electric push rods, and two turnover auxiliary mechanisms symmetrically connected to the inner wall of the belt conveyor, wherein the electric push rods are used for adjusting the distance between the reagent box turnover mechanism and the turnover auxiliary mechanisms;
the reagent box tilting mechanism includes supporting component, directional rotation subassembly, connects two telescopic spacing subassemblies on directional rotation subassembly and locates the tensile regulation subassembly in the directional rotation subassembly, two telescopic spacing subassemblies all are connected with tensile regulation subassembly, and two telescopic spacing subassemblies are used for the centre gripping to wait the reagent box of upset, the upset auxiliary mechanism includes first adjusting component and second adjusting component, first adjusting component is used for with directional rotation subassembly contact and drive directional rotation subassembly rotation in the contact process, the second adjusting component is used for with tensile regulation subassembly contact and drive tensile regulation subassembly operation in the contact process, tensile regulation subassembly operation control corresponding telescopic spacing subassembly shortens.
As a further optimization scheme of the application, the belt conveyor comprises a support frame body, a belt conveying assembly arranged in the support frame body, an upper through groove and a lower through groove which are respectively arranged at the upper end and the lower end of the support frame body, a first range finder and a second range finder are arranged at the upper through groove, an electric push rod is arranged at the lower through groove, two electric push rods are arranged at two sides of the belt conveying assembly, and two overturning auxiliary mechanisms are symmetrically connected to the inner wall of the upper through groove and are matched with two reagent box overturning mechanisms.
As a further optimization scheme of the application, the supporting component comprises a supporting plate, an adjusting cavity arranged in the supporting plate and a first damping ring fixedly connected to the side wall of the supporting plate, wherein the adjusting cavity is communicated with the outer space of the supporting plate through the first damping ring, the supporting plate is connected to the output end of the electric push rod, and a slot for inserting the first adjusting component and the second adjusting component is arranged at the upper end of the supporting plate.
As a further optimization scheme of the application, the directional rotating assembly comprises a fixed plate, a hollow connecting pipe movably connected with the fixed plate, a pawl connected with the side wall of the fixed plate, a ratchet connected with the hollow connecting pipe and a connecting plate connected with one end of the hollow connecting pipe, one end of the hollow connecting pipe penetrates through the first damping ring and is fixedly connected with the connecting plate, a second damping ring matched with the first damping ring is arranged on the outer wall of the hollow connecting pipe, and a third damping ring is connected with the outer wall of the hollow connecting pipe at a position close to the other end of the hollow connecting pipe.
As a further optimization scheme of the application, the telescopic limiting assembly comprises a T-shaped plate, a limiting sleeve plate sleeved at one end of the T-shaped plate, a spring connected between the T-shaped plate and the limiting sleeve plate and a rope connected to the inner wall of the limiting sleeve plate, wherein the T-shaped plate is fixedly connected to the connecting plate, and one end of the rope sequentially penetrates through the spring, the T-shaped plate, the connecting plate and the hollow connecting pipe and is connected to the stretching adjusting assembly.
As a further optimization scheme of the application, the stretching adjusting component comprises a fixing frame and two I-shaped bobbins movably connected to the fixing frame, the fixing frame is connected to the position, close to the other end, of the inner wall of the hollow connecting pipe, a winding part and a limiting part are arranged on the I-shaped bobbins, one end of the rope is connected to the winding part of the corresponding I-shaped bobbin, and a first damping layer is arranged on the limiting part of the I-shaped bobbins.
As a further optimization scheme of the application, the first adjusting component comprises a first cutting and a second damping layer arranged on the first cutting, the first cutting is tangential to the hollow connecting pipe, and the second damping layer is matched with the third damping ring.
As a further optimization scheme of the application, the second adjusting component comprises a second cutting and a third damping layer arranged on the second cutting, the second cutting is tangential to the limiting part of the I-shaped winding drum, the third damping layer is matched with the first damping layer, the length of the second cutting is greater than that of the first cutting, and the highest point of the third damping layer is lower than the lowest point of the second damping layer.
The application method of the fluorescent immunochromatography rapid quantitative detection device comprises the following steps of:
placing the reagent boxes in a vibrating tray type feeding machine, arranging and conveying the reagent boxes into a belt type conveyor through the vibrating tray type feeding machine, acquiring space data of the reagent boxes passing through the position right below the first distance measuring instrument sequentially through the first distance measuring instrument, judging whether the corresponding reagent boxes need to be overturned or not through the corresponding space data, and stopping running when the belt type conveyor conveys the corresponding reagent boxes to an adjusting mechanism when the corresponding reagent boxes need to be overturned;
the reagent box turnover mechanism is pushed to move upwards by the electric push rod, in the upward moving process, the reagent box to be turned over is turned over for one hundred eighty degrees by the reagent box turnover mechanism and the turnover auxiliary mechanism, then the electric push rod drives the reagent box turnover mechanism to move downwards for resetting, and the turned reagent box moves towards the second range finder along with the belt conveying assembly and is re-checked by the second range finder, and is transmitted to the sample detector after being re-checked without errors;
the sample detector drops different sample mixtures into corresponding kits and conveys the kits dropped into the sample mixtures into an observation chamber to observe detection results.
The application has the beneficial effects that: according to the application, the adjusting mechanism is arranged on the belt conveyor, when the corresponding reagent box needs to be overturned, the reagent box overturning mechanism is pushed to move towards the reagent box to be overturned by the electric push rod and is matched with the overturning auxiliary mechanism, so that the reagent box can be overturned for one hundred and eighty degrees, and the whole process only needs one power source.
Drawings
FIG. 1 is a schematic view of the overall structure of the present application;
FIG. 2 is a mating view of the belt conveyor and adjustment mechanism of the present application;
FIG. 3 is a schematic diagram of the structure of the turnover mechanism of the kit of the present application;
FIG. 4 is an enlarged view of the application at A in FIG. 3;
FIG. 5 is a mating view of the ratchet and pawl of the present application;
fig. 6 is a mating view of the present i-shaped bobbin and a second slip.
In the figure: 1. a vibrating disc type feeding machine; 2. a sample detector; 3. a belt conveyor; 301. a support frame body; 302. a belt conveyor assembly; 303. a lower through groove; 304. an upper through groove; 41. a first range finder; 42. a second range finder; 43. a counter; 5. an adjusting mechanism; 51. a control box; 52. an electric push rod; 53. a reagent box turnover mechanism; 5301. a support plate; 5302. regulating the chamber; 5303. a fixing plate; 5304. a first damping ring; 5305. a ratchet wheel; 5306. a pawl; 5307. a hollow connecting pipe; 5308. a connecting plate; 5309. a T-shaped plate; 5310. a limiting sleeve plate; 5311. a spring; 5312. a rope; 5313. a fixing frame; 5314. an I-shaped bobbin; 5315. a second damping ring; 5316. a third damping ring; 5317. a first damping layer; 54. a turnover auxiliary mechanism; 5401. a first slip; 5402. a second cutting; 5403. a second damping layer; 5404. and a third damping layer.
Detailed Description
The present application will be described in further detail with reference to the accompanying drawings, wherein it is to be understood that the following detailed description is for the purpose of further illustrating the application only and is not to be construed as limiting the scope of the application, as various insubstantial modifications and adaptations of the application to those skilled in the art can be made in light of the foregoing disclosure.
Example 1
As shown in fig. 1-6, a fluorescence immunochromatography rapid quantitative detection device comprises a vibration disc type feeding machine 1, a sample detection machine 2, a belt conveyor 3 arranged between the vibration disc type feeding machine 1 and the sample detection machine 2, a data acquisition mechanism arranged on the belt conveyor 3 and an adjusting mechanism 5 arranged below the belt conveyor 3, wherein the vibration disc type feeding machine 1 is used for conveying a reagent box onto the belt conveyor 3, and the belt conveyor 3 is used for conveying the reagent box into the sample detection machine 2;
the data acquisition mechanism comprises a first distance meter 41, a second distance meter 42 and a counter 43 which are sequentially arranged on the belt conveyor 3, wherein the first distance meter 41 and the second distance meter 42 are used for acquiring the space data of the kit;
the adjusting mechanism 5 comprises a control box 51 arranged below the belt conveyor 3, two electric push rods 52 symmetrically connected to the upper end of the control box 51, a reagent box turnover mechanism 53 connected to the output end of the electric push rods 52, and two turnover auxiliary mechanisms 54 symmetrically connected to the inner wall of the belt conveyor 3, wherein the electric push rods 52 are used for adjusting the distance between the reagent box turnover mechanism 53 and the turnover auxiliary mechanisms 54;
the reagent box tilting mechanism 53 includes supporting component, directional rotating component, connect two telescopic spacing subassemblies on directional rotating component and locate the tensile regulation subassembly in the directional rotating component, two telescopic spacing subassemblies all are connected with tensile regulation subassembly, two telescopic spacing subassemblies are used for the centre gripping to wait the reagent box of upset, upset auxiliary mechanism 54 includes first adjusting component and second adjusting component, first adjusting component is used for with directional rotating component contact and drive directional rotating component rotation in the contact process, the second adjusting component is used for with tensile regulation subassembly contact and drive tensile regulation subassembly operation in the contact process, tensile regulation subassembly operation control corresponding telescopic spacing subassembly shortens.
It should be noted that, the reagent boxes are placed in the vibrating tray type feeding machine 1, the reagent boxes are arranged and conveyed into the belt conveyor 3 through the vibrating tray type feeding machine 1, the space data of the reagent boxes passing through the position right below the first distance measuring instrument 41 in sequence are obtained through the first distance measuring instrument 41, whether the corresponding reagent boxes need to be overturned or not is judged through the corresponding space data, and when the corresponding reagent boxes need to be overturned, the belt conveyor 3 stops running when conveying the corresponding reagent boxes to the position of the adjusting mechanism 5;
the reagent box turnover mechanism 53 is pushed to move upwards by the electric push rod 52, in the upward moving process, the reagent box to be turned is turned over by one hundred eighty degrees by the reagent box turnover mechanism 53 and the turnover auxiliary mechanism 54, then the electric push rod 52 drives the reagent box turnover mechanism 53 to move downwards for resetting, specifically, the second adjusting component is firstly inserted into the supporting component and contacted with the corresponding stretching adjusting component, and drives the telescopic limiting component positioned above to shorten in the contacting process until the telescopic limiting component positioned below contacts the reagent box to be turned over and drives the reagent box to be turned over to move in the same direction, and at the moment, the telescopic limiting component positioned above resets and cooperates with the telescopic limiting component positioned below to clamp the reagent box to be turned over, and the reagent box to be turned over continues to move upwards;
when the first adjusting component is inserted into the supporting component and contacts with the directional rotating component, the first adjusting component drives the directional rotating component, the two telescopic limiting components connected to the directional rotating component, the stretching adjusting component and the reagent box limited between the two telescopic limiting components to directionally rotate for one hundred eighty degrees, at this time, the electric push rod 52 drives the reagent box turnover mechanism 53 to move back, in the process, the directional rotating component is gradually separated from the first adjusting component, the second adjusting component drives the telescopic limiting component positioned above to shorten through the stretching adjusting component again until the turned reagent box contacts with the belt conveying component 302 and continuously moves downwards, the turned reagent box is gradually separated from the telescopic limiting component positioned below under the limiting action of the belt conveying component 302, the telescopic limiting component positioned above is in a shortened state, the turned reagent box cannot be contacted in the continuous downwards moving process until the reagent box moves to the lower side of the turned reagent box, the stretching adjusting component is separated from the second adjusting component, the telescopic limiting component positioned above is reset and moves to the initial position, the reagent box is continuously moved to the second distance measuring instrument 42, and the second distance measuring instrument is continuously moved to the position of the second distance measuring instrument 42 after the second distance measuring instrument is continuously moved to the position of the second distance measuring instrument, and the distance measuring instrument is not subjected to the error detection and is 2;
the sample detector 2 drops different sample mixtures into corresponding reagent boxes and conveys the reagent boxes dropped into the sample mixtures into an observation chamber to observe the detection result.
It should be noted that, the sample detector 2, the vibrating tray feeder 1 and the belt conveyor 3 are all of the prior art, and the specific internal structure and the equipment model thereof are not described herein.
The belt conveyor 3 includes a supporting frame 301, a belt conveying assembly 302 disposed in the supporting frame 301, and an upper through groove 304 and a lower through groove 303 respectively disposed at the upper end and the lower end of the supporting frame 301, the first range finder 41 and the second range finder 42 are disposed at the upper through groove 304, the electric push rod 52 is disposed at the lower through groove 303, and two electric push rods 52 are disposed at two sides of the belt conveying assembly 302, and the two overturning auxiliary mechanisms 54 are symmetrically connected to the inner wall of the upper through groove 304 and are disposed in a matching manner with the two reagent kit overturning mechanisms 53.
It should be noted that, as described above, the belt conveyor assembly 302 receives the reagent kit conveyed from the vibrating tray feeder 1 and conveys the reagent kit towards the sample detector 2, in this process, the reagent kit sequentially passes through the first distance meter 41, the second distance meter 42 and the counter 43, when passing through the first distance meter 41, the first distance meter 41 can detect the surface roughness of the reagent kit, that is, detect whether the reagent kit drip port is upward, if upward, the reagent kit drip port does not need to be turned over, if no space data of the reagent kit drip port is detected, that is, the reagent kit drip port is downward contacted with the belt conveyor assembly 302, the reagent kit will be affected to enter the subsequent use in the sample detector 2, therefore, after the first distance meter 41 detects the reagent kit needing to be turned over, the belt conveyor 3 stops conveying the corresponding reagent kit to the position of the adjusting mechanism 5, then pushes the reagent kit turning mechanism 53 to move upward by the electric push rod 52, in the upward moving process, the reagent kit to be turned over by one hundred eighty degrees by the reagent kit turning mechanism 53 and the turning auxiliary mechanism 54, and then the electric push rod 52 drives the reagent kit turning mechanism 53 to move downward to reset to complete the whole turning process.
The support assembly includes a support plate 5301, an adjusting chamber 5302 disposed in the support plate 5301, and a first damping ring 5304 fixedly connected to a side wall of the support plate 5301, wherein the adjusting chamber 5302 is spatially communicated with an outside of the support plate 5301 through the first damping ring 5304, the support plate 5301 is connected to an output end of the electric push rod 52, and a slot into which the first adjusting assembly and the second adjusting assembly are inserted is disposed at an upper end of the support plate 5301;
the directional rotation assembly comprises a fixed plate 5303, a hollow connecting pipe 5307 movably connected with the fixed plate 5303, a pawl 5306 connected with the side wall of the fixed plate 5303, a ratchet 5305 connected with the hollow connecting pipe 5307 and a connecting plate 5308 connected with one end of the hollow connecting pipe 5307, wherein one end of the hollow connecting pipe 5307 passes through a first damping ring 5304 and is fixedly connected with the connecting plate 5308, a second damping ring 5315 matched with the first damping ring 5304 is arranged on the outer wall of the hollow connecting pipe 5307, and a third damping ring 5316 is connected to the outer wall of the hollow connecting pipe 5307 at a position close to the other end; the cooperation of the first damping ring 5304 and the second damping ring 5315 can enable the hollow connecting pipe 5307 to be stable under the condition that the hollow connecting pipe 5307 is not subjected to the adjusting acting force of the first adjusting component and cannot rotate, meanwhile, through the cooperation of the pawl 5306 and the ratchet 5305, the hollow connecting pipe 5307 can only rotate along one direction under the adjusting action of the first adjusting component, and the hollow connecting pipe 5307 can be prevented from being driven to rotate reversely when the first adjusting component is pulled away;
the telescopic limiting assembly comprises a T-shaped plate 5309, a limiting sleeve plate 5310 sleeved at one end of the T-shaped plate 5309, a spring 5311 connected between the T-shaped plate 5309 and the limiting sleeve plate 5310, a rope 5312 connected to the inner wall of the limiting sleeve plate 5310, wherein the T-shaped plate 5309 is fixedly connected to the connecting plate 5308, and one end of the rope 5312 sequentially penetrates through the spring 5311, the T-shaped plate 5309, the connecting plate 5308 and the hollow connecting pipe 5307 and is connected to the stretching adjusting assembly.
The stretching adjusting component comprises a fixing frame 5313 and two I-shaped bobbins 5314 movably connected to the fixing frame 5313, the fixing frame 5313 is connected to the inner wall of the hollow connecting pipe 5307 at a position close to the other end, a winding part and a limiting part are arranged on the I-shaped bobbins 5314, one end of a rope 5312 is connected to the winding part of the corresponding I-shaped bobbin 5314, and a first damping layer 5317 is arranged on the limiting part of the I-shaped bobbin 5314.
It should be noted that, as described above, when the electric push rod 52 pushes the reagent cartridge tilting mechanism 53 to move upward, during which the second regulating member is preferentially inserted into the regulating chamber 5302 of the supporting plate 5301, when the second regulating member contacts the first damping layer 5317 provided on the stopper portion of the i-shaped bobbin 5314 in the tension regulating member, the second regulating member drives the i-shaped bobbin 5314 to start rotating by the frictional force generated between the second regulating member and the first damping layer 5317, because the position in which the second regulating member is inserted is fixed and contacts only one of the i-shaped bobbins 5314, i.e., contacts only the i-shaped bobbin 5314 connected to the upper telescopic stopper member, at this time, the i-shaped bobbin 5314 rotates and starts gradually winding the rope 5312 in the upper telescopic stopper member, the rope 5312 gradually winds around the i-shaped bobbin 5314, the limiting sleeve plate 5310 at the upper position is gradually pulled to move towards the direction of the connecting plate 5308 until the upper limiting sleeve plate 5310 moves to the lower end face of the reagent box to be turned, the retracting distance of the limiting sleeve plate 5310 is just not contacted with the reagent box to be turned, at the moment, the limiting sleeve plate 5310 at the upper position continuously moves upwards, the limiting sleeve plate 5310 at the lower position continuously retracts until friction force is not generated between the second adjusting component and the first damping layer 5317 on the I-shaped winding reel 5314 when the lower limiting sleeve plate 5310 contacts the lower end face of the reagent box to be turned, at the moment, the limiting sleeve plate 5310 at the upper position resets under the action of deformation elasticity of the spring 5311 and contacts the upper end face of the reagent box to be turned, at the moment, the two limiting sleeve plates 5310 distributed up and down just limit and clamp the reagent box to be turned, then the supporting plate 5301 continuously moves upwards for a certain distance, the limiting sleeve plate 5310 clamps the reagent box to move upwards together, until the first adjusting component contacts with the third damping ring 5316 on the hollow connecting tube 5307 in the directional rotating component and generates a corresponding friction force which is larger than the friction force generated between the first damping ring 5304 and the second damping ring 5315, along with the upward movement process, after the first adjusting component drives the hollow connecting tube 5307 to rotate for one hundred eighty degrees, the ratchet 5305 rotates for one hundred eighty degrees along with the hollow connecting tube 5307 towards the non-limiting rotation direction of the pawl 5306, the connecting plate 5308, the limiting sleeve plate 5310 for clamping the reagent box and the reagent box all rotate for one hundred eighty degrees along with the hollow connecting tube 5307, after overturning, the friction force is not generated between the first adjusting component and the third damping ring 5316 any more, the electric push rod 52 reaches the stroke limit and starts to move back, the first adjusting component and the third damping ring 5316 generate friction force again, but under the limiting effect of the pawl 5306 and the ratchet 5305, the hollow connecting tube 5307 does not rotate reversely any more, the turned-over reagent box can be stably moved downwards until the first regulating component is completely separated from the third damping ring 5316, and continuously moved downwards, at the moment, the lower limit sleeve plate 5310 is positioned at the current upper position after one hundred eighty degrees of rotation, the H-shaped winding tube 5314 connected with the lower limit sleeve plate 5310 is also rotated to the position contacted with the second regulating component, along with the moving downwards, the H-shaped winding tube 5314 starts to rotate and pulls the upper limit sleeve plate 5310 to retract through the rope 5312, when the turned-over reagent box is contacted with the belt conveying component 302, the upper limit sleeve plate 5310 is not contacted with the turned-over reagent box any more, the moving downwards process of the supporting plate 5301 is not influenced, when the upper limit sleeve plate 5310 is moved to the lower part of the turned-over reagent box, the second regulating component is separated from the H-shaped winding tube 5314, the springs 5311 spring back and return the corresponding stop collar plate 5310 and the entire flipping process ends until the beginning of the next flipping process repeats the above process.
The first adjusting component includes a first insert 5401 and a second damping layer 5403 disposed on the first insert 5401, the first insert 5401 is tangential to the hollow connecting tube 5307, and the second damping layer 5403 is matched with the third damping ring 5316.
It should be noted that, as described above, the second damping layer 5403 generates a corresponding friction force and drives the hollow connection tube 5307 to rotate when contacting the third damping ring 5316, and the length and the friction force of the second damping layer 5403 are determined according to the specific values calculated during actual production.
The second adjusting component includes a second insert 5402 and a third damping layer 5404 disposed on the second insert 5402, the second insert 5402 is tangential to a limiting portion of the i-shaped bobbin 5314, the third damping layer 5404 is disposed in matching with the first damping layer 5317, the length of the second insert 5402 is greater than that of the first insert 5401, and a highest point of the third damping layer 5404 is lower than a lowest point of the second damping layer 5403.
As described above, the third damping layer 5404 on the second insert 5402 generates a friction force when contacting the first damping layer 5317 provided on the i-shaped bobbin 5314, and drives the i-shaped bobbin 5314 to rotate, and the friction force is required to overcome the elastic force of the spring 5311 gradually changing when driving the i-shaped bobbin 5314 to rotate.
The above examples merely represent a few embodiments of the present application, which are described in more detail and are not to be construed as limiting the scope of the present application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application.
Claims (3)
1. A fluorescence immunochromatography rapid quantitative detection device is characterized in that: the device comprises a vibration disc type feeding machine (1), a sample detection machine (2), a belt conveyor (3) arranged between the vibration disc type feeding machine (1) and the sample detection machine (2), a data acquisition mechanism arranged on the belt conveyor (3) and an adjusting mechanism (5) arranged below the belt conveyor (3), wherein the vibration disc type feeding machine (1) is used for conveying a reagent box onto the belt conveyor (3), and the belt conveyor (3) is used for conveying the reagent box into the sample detection machine (2);
the data acquisition mechanism comprises a first range finder (41), a second range finder (42) and a counter (43) which are sequentially arranged on the belt conveyor (3), wherein the first range finder (41) and the second range finder (42) are both used for acquiring the space data of the kit;
the adjusting mechanism (5) comprises a control box (51) arranged below the belt conveyor (3), two electric push rods (52) symmetrically connected to the upper end of the control box (51), a reagent box turnover mechanism (53) connected to the output end of the electric push rods (52) and two turnover auxiliary mechanisms (54) symmetrically connected to the inner wall of the belt conveyor (3), wherein the electric push rods (52) are used for adjusting the distance between the reagent box turnover mechanism (53) and the turnover auxiliary mechanisms (54);
the reagent box overturning mechanism (53) comprises a supporting component, an oriented rotating component, two telescopic limiting components connected to the oriented rotating component and a stretching adjusting component arranged in the oriented rotating component, wherein the two telescopic limiting components are connected with the stretching adjusting component, the two telescopic limiting components are used for clamping a reagent box to be overturned, the overturning assisting mechanism (54) comprises a first adjusting component and a second adjusting component, the first adjusting component is used for being in contact with the oriented rotating component and driving the oriented rotating component to rotate in the contact process, the second adjusting component is used for being in contact with the stretching adjusting component and driving the stretching adjusting component to rotate in the contact process, and the stretching adjusting component is controlled to be shortened in the operation;
the supporting component comprises a supporting plate (5301), an adjusting cavity (5302) arranged in the supporting plate (5301) and a first damping ring (5304) fixedly connected to the side wall of the supporting plate (5301), the adjusting cavity (5302) is communicated with the outer space of the supporting plate (5301) through the first damping ring (5304), the supporting plate (5301) is connected to the output end of the electric push rod (52), and a slot for inserting the first adjusting component and the second adjusting component is formed in the upper end of the supporting plate (5301);
the directional rotating assembly comprises a fixed plate (5303), a hollow connecting pipe (5307) movably connected with the fixed plate (5303), a pawl (5306) connected with the side wall of the fixed plate (5303), a ratchet wheel (5305) connected with the hollow connecting pipe (5307) and a connecting plate (5308) connected with one end of the hollow connecting pipe (5307), one end of the hollow connecting pipe (5307) passes through a first damping ring (5304) and is fixedly connected with the connecting plate (5308), a second damping ring (5315) matched with the first damping ring (5304) is arranged on the outer wall of the hollow connecting pipe (5307), and a third damping ring (5316) is connected to the outer wall of the hollow connecting pipe (5307) at a position close to the other end;
the telescopic limiting assembly comprises a T-shaped plate (5309), a limiting sleeve plate (5310) sleeved at one end of the T-shaped plate (5309), a spring (5311) connected between the T-shaped plate (5309) and the limiting sleeve plate (5310) and a rope (5312) connected to the inner wall of the limiting sleeve plate (5310), the T-shaped plate (5309) is fixedly connected to the connecting plate (5308), and one end of the rope (5312) sequentially penetrates through the spring (5311), the T-shaped plate (5309), the connecting plate (5308) and the hollow connecting pipe (5307) and is connected to the stretching adjusting assembly;
the stretching adjusting component comprises a fixing frame (5313) and two I-shaped bobbins (5314) which are movably connected to the fixing frame (5313), the fixing frame (5313) is connected to the position, close to the other end, of the inner wall of the hollow connecting pipe (5307), a winding part and a limiting part are arranged on the I-shaped bobbins (5314), one end of a rope (5312) is connected to the winding part of the corresponding I-shaped bobbin (5314), and a first damping layer (5317) is arranged on the limiting part of the I-shaped bobbins (5314);
the first adjusting component comprises a first cutting (5401) and a second damping layer (5403) arranged on the first cutting (5401), the first cutting (5401) is tangential to the hollow connecting pipe (5307), and the second damping layer (5403) is matched with the third damping ring (5316);
the second adjusting component comprises a second cutting (5402) and a third damping layer (5404) arranged on the second cutting (5402), the second cutting (5402) is tangentially arranged with a limiting part of the I-shaped winding drum (5314), the third damping layer (5404) is arranged in a matched mode with the first damping layer (5317), the length of the second cutting (5402) is larger than that of the first cutting (5401), and the highest point of the third damping layer (5404) is lower than the lowest point of the second damping layer (5403).
2. The rapid quantitative fluorescent immunochromatographic assay device according to claim 1, wherein: the belt conveyor (3) comprises a supporting frame body (301), a belt conveying assembly (302) arranged in the supporting frame body (301), an upper through groove (304) and a lower through groove (303) which are respectively formed in the upper end and the lower end of the supporting frame body (301), a first distance meter (41) and a second distance meter (42) are arranged at the upper through groove (304), an electric push rod (52) is arranged at the lower through groove (303), two electric push rods (52) are arranged on two sides of the belt conveying assembly (302), and two overturning auxiliary mechanisms (54) are symmetrically connected on the inner wall of the upper through groove (304) and are arranged in a matched mode with two reagent box overturning mechanisms (53).
3. A method of using a rapid quantitative fluorescent immunochromatographic assay device according to any one of claims 1 to 2, comprising the steps of:
the method comprises the steps that a reagent box is placed in a vibrating disc type feeding machine (1), the reagent box is arranged and conveyed into a belt type conveyor (3) through the vibrating disc type feeding machine (1), space data of the reagent box passing through the position right below the first distance measuring instrument (41) are obtained through the first distance measuring instrument (41), whether the corresponding reagent box needs to be overturned or not is judged through the corresponding space data, and when the corresponding reagent box needs to be overturned, the belt type conveyor (3) stops running when the corresponding reagent box is conveyed to an adjusting mechanism (5);
the reagent box turnover mechanism (53) is pushed by the electric push rod (52) to move upwards, in the upward moving process, the reagent box to be turned is turned over for one hundred eighty degrees by the reagent box turnover mechanism (53) and the turnover auxiliary mechanism (54), then the electric push rod (52) drives the reagent box turnover mechanism (53) to move downwards for resetting, and the turned reagent box moves towards the second range finder (42) along with the belt conveying component (302) and is subjected to rechecking by the second range finder (42), and is transmitted to the sample detector (2) after rechecking;
the sample detector (2) drops different sample mixtures into corresponding kits and conveys the kits dropped into the sample mixtures into an observation chamber to observe detection results.
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