CN113865962A - Specific protein analysis equipment and method - Google Patents
Specific protein analysis equipment and method Download PDFInfo
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- CN113865962A CN113865962A CN202111178411.6A CN202111178411A CN113865962A CN 113865962 A CN113865962 A CN 113865962A CN 202111178411 A CN202111178411 A CN 202111178411A CN 113865962 A CN113865962 A CN 113865962A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
Abstract
The invention discloses specific protein analysis equipment and a specific protein analysis method, and relates to the technical field of specific protein analysis. The rotary incubation mechanism is used for accommodating, rotationally oscillating and incubating a plurality of detection cups, the plurality of clamping and transferring mechanisms are used for simultaneously clamping and transferring the plurality of detection cups, the temporary storage and transportation mechanism is used for temporarily storing and transporting the plurality of detection cups, the clamping and transferring mechanism is used for sequentially clamping and transferring the plurality of detection cups to the position of the adjusting and collecting mechanism, and the detection liquid in the detection cups is subjected to detection and analysis of specific proteins. The specific protein analysis equipment realizes simultaneous accommodation, rotary oscillation and incubation of a plurality of detection cups, and transports, detects and collects the plurality of detection cups. The specific protein analysis method performs analysis of multiple specific proteins by matching the single light sources with different wavelengths for illumination with the corresponding silicon photocells, is suitable for synchronously detecting multiple specific protein analysis items of different detection solutions, and has high analysis efficiency and wide application range.
Description
Technical Field
The invention relates to the technical field of specific protein analysis, in particular to specific protein analysis equipment and a specific protein analysis method.
Background
The analyzer is one of the common apparatuses for medical detection tests, and can perform analysis and measurement of various reaction types such as a timer method and a continuous monitoring method. Besides general project determination, some methods can also be used for determining special compounds such as hormone, immunoglobulin, blood concentration and the like and analyzing methods such as enzyme immunity, fluorescence immunity and the like, and have the characteristics of rapidness, convenience, sensitivity, accuracy, standardization, trace and the like. Among them, the analyzer for specific proteins is mainly used for detecting the concentration of specific proteins in serum, plasma and urine.
The specific protein analyzer in the prior art comprises an incubation unit, a detection unit, a driving mechanism, a card reader, a two-dimensional code/bar code scanner, a printer and a control unit, wherein the incubation unit comprises a first incubation device and a first sample rack, and a plurality of incubation holes are arranged on the first sample rack; the detection unit comprises a second incubator, a second sample rack arranged on the second incubator, a light source and a data acquisition unit which are respectively arranged at two sides of the second sample rack, and a plurality of detection holes which are arranged in a straight line are arranged on the second sample rack; the driving mechanism is used for driving the light source and the data acquisition unit to synchronously move to a position corresponding to one of the detection holes. Although the correlation between the detection information and the result can be realized and the printing can be performed, the following technical problems exist: 1) the method can not be used for simultaneously accommodating, rotationally oscillating and incubating a plurality of detection cups, and is not suitable for synchronously detecting a plurality of specific protein analysis items of detection liquid; 2) the detection liquid is easy to settle and deteriorate in the transportation process to be detected, and the accuracy of specific protein analysis is influenced.
Disclosure of Invention
The invention aims to provide specific protein analysis equipment and a specific protein analysis method, which are used for solving the technical problems that in the prior art, a plurality of detection cups cannot be accommodated, rotationally oscillated and incubated at the same time, the equipment is not suitable for synchronously detecting a plurality of specific protein analysis items of detection liquid, and the detection liquid is easy to settle and deteriorate in the transportation process to be detected, so that the accuracy of specific protein analysis is influenced.
The purpose of the invention can be realized by the following technical scheme:
the invention provides specific protein analysis equipment which comprises a lower box body and an upper cover body covering the lower box body, wherein a rotary incubation mechanism for accommodating, rotationally oscillating and incubating a plurality of detection cups is arranged on the lower box body;
the rotary incubation mechanism comprises a fixed disc and a rotary incubation table, a cavity is formed in the fixed disc, the rotary incubation table is arranged in the cavity of the fixed disc in a sliding mode, a plurality of accommodating cavities used for accommodating detection cups are distributed on the upper surface of the rotary incubation table in an annular array mode, a first speed regulating motor is arranged at the center bottom of the fixed disc, and a motor shaft of the first speed regulating motor is connected with a rotating shaft penetrating through the fixed disc to the lower surface of the rotary incubation table through a coupler; the inner cavity of the fixed disc is provided with a heat conduction cavity, and a heat conduction medium is introduced into the heat conduction cavity.
Furthermore, two sides of the bottom of the fixed disc are connected with L-shaped brackets, the upper surface of the center of the rotary incubation table is provided with a stabilizing rod, and the bottom end of the stabilizing rod is in threaded connection with the top end of the rotary shaft; the accommodating cavity is in interference fit with the detection cup, and the height of the detection cup is greater than that of the accommodating cavity; the bottom of the containing cavity is provided with a plurality of heat conduction pipes communicated with the heat conduction cavity.
Further, rotatory incubation mechanism, transportation temporary storage mechanism, regulation collection mechanism are located directly over to a plurality of centre gripping transport mechanism, centre gripping transport mechanism includes second buncher, ball, nut seat, lift centre gripping oscillating mechanism, and second buncher passes through the shaft coupling and is connected with ball, and the inner chamber of nut seat is equipped with the internal thread with ball external screw thread fit, and lift centre gripping oscillating mechanism installs in the below of nut seat, and ball's end-to-end connection has the bearing frame.
Further, lift centre gripping oscillating mechanism includes flexible cylinder, switching platform, oscillation chamber, and the below of nut seat is located to flexible cylinder, and switching platform fixed connection is in the below of flexible cylinder, and the below of switching platform is located to the oscillation chamber, is equipped with servo motor in the oscillation chamber, and servo motor's motor shaft is connected with the centre gripping platform after stretching out the oscillation chamber downwards.
Furthermore, a clamping cavity is arranged in the clamping table, and the inner size of the clamping cavity is matched with the outer size of the detection cup; a plurality of damping springs are symmetrically arranged between the shell of the servo motor and the inner wall of the oscillation chamber.
Furthermore, the temporary transport storage mechanism comprises a transmission belt which is arranged in a surrounding manner, a plurality of transmission rollers are arranged in the transmission belt at equal intervals, and one transmission roller at the end part is driven to rotate by a driving device; two sides of the length direction of the conveying belt are provided with annular limiting plates, and the size between the two corresponding limiting plates is matched with the peripheral size of the detection cup.
Further, adjust collection mechanism and include third buncher, plummer, the cross-section of plummer is the U-shaped, and the both sides of plummer are equipped with first light screen and second light screen respectively, and third buncher is connected with the axis of rotation that runs through first light screen to the second light screen outside, and the outside of first light screen is located to third buncher, and the inner wall that the axis of rotation periphery is located first light screen is equipped with the light source board, and the inner wall that the axis of rotation periphery is located the second light screen is equipped with the photoelectricity and gathers the board.
Furthermore, the first shading plate and the second shading plate are higher than the bearing table, the upper surface of the bearing table is provided with a detection table, and a detection cavity for accommodating the detection cup is arranged on the detection table; a first through hole for the rotating shaft to penetrate through is formed in the center of the light source plate, and a plurality of single light sources emitting light with different wavelengths are distributed on one surface, close to the detection table, of the light source plate in an annular array mode; a second through hole for the rotating shaft to penetrate through is formed in the center of the photoelectric acquisition board, and a plurality of silicon photocells are distributed on one surface, close to the detection table, of the photoelectric acquisition board in an annular array manner; the number of the silicon photocells and the single light sources is the same and corresponds to one.
The invention also provides a specific protein analysis method, which comprises the following steps:
s1, sample loading and incubation: diluting human blood or urine to be detected by a solvent to obtain a detection liquid, putting the detection liquid into a detection cup, and covering and sealing the detection cup by an upper cover; placing a plurality of detection cups into the accommodating cavities, introducing a heat-conducting medium into the heat-conducting cavities, conducting heat into each accommodating cavity by the heat-conducting medium along the rotary incubation table, and synchronously heating and incubating detection liquids in the detection cups;
s2, synchronous oscillation: the first speed regulating motor drives the rotating shaft to rotate through the coupler, and the rotating shaft drives the rotating incubation table to rotate, so that detection liquid in the detection cups synchronously oscillate;
s3, clamping and transferring: the second speed regulating motors drive the ball screws to rotate, nut seats meshed with the ball screws move along the axial directions of the ball screws, and the nut seats drive the lifting clamping oscillating mechanisms to move synchronously; when the lifting clamping oscillating mechanism moves to the position above the detection cup, the telescopic cylinder extends downwards, and a clamping cavity in the clamping table clamps the detection cup; when the telescopic cylinder contracts upwards and the nut seat drives the lifting clamping oscillating mechanism to move to the position above the temporary transportation storage mechanism, the telescopic cylinder extends downwards, and the clamping table places the detection cup in the annular limiting plate; repeating the step S3, and temporarily storing a plurality of detection cups on the conveying belt;
s4, secondary oscillation and transfer: the transmission belt is driven around the transmission rollers to transmit the detection cup forwards; when the lifting clamping oscillation mechanism moves to the position above the detection cup, the telescopic cylinder extends downwards, a clamping cavity in the clamping table clamps the detection cup, the servo motor drives the clamping table to rotate, and secondary oscillation mixing is carried out on detection liquid in the detection cup; the nut seat drives the lifting clamping oscillation mechanism to move above the detection table, and the telescopic cylinder extends downwards to place the detection cup in the detection cavity;
s5, detection: starting a third speed regulating motor to drive the rotating shaft to rotate, so that the light source plate and the photoelectric acquisition plate rotate along with the rotating shaft, and the light source plate and the photoelectric acquisition plate are linked in a consistent manner; light emitted by the monomer light source passes through the light inlet hole on the detection cup and is emitted to the corresponding silicon photocell from the light outlet hole, and specific protein is analyzed; and the single light sources with different wavelengths for illumination are matched with corresponding silicon photocells to analyze a plurality of specific proteins.
Further, after the detection is finished, a clamping cavity in the clamping table clamps the detection cup, the nut seat drives the lifting clamping oscillation mechanism to move to the position above the rotary incubation table, and the telescopic cylinder extends downwards to place the detection cup in the accommodating cavity; repeating the steps S4-S5, and performing specific protein analysis on the detection solution in the plurality of detection cups.
The invention has the following beneficial effects:
1. according to the specific protein analysis equipment, the rotary incubation mechanism is used for accommodating, rotationally oscillating and incubating a plurality of detection cups, the plurality of clamping and transferring mechanisms are used for simultaneously clamping and transferring the plurality of detection cups, the temporary transportation storage mechanism is used for temporarily storing and transporting the detection cups, the clamping and transferring mechanism is used for sequentially clamping and transferring the detection cups to the position of the adjusting and collecting mechanism, and the detection solution in the detection cups is subjected to specific protein detection and analysis. The specific protein analysis equipment realizes simultaneous accommodation, rotary oscillation and incubation of a plurality of detection cups, transports, detects and collects the plurality of detection cups, and is suitable for synchronously detecting various specific protein analysis items of different detection liquids.
2. The clamping chamber in the telescopic cylinder drives the clamping table to clamp the detection cup, and the servo motor drives the clamping table to rotate, so that secondary oscillation mixing is performed on detection liquid in the detection cup, and the detection liquid is prevented from settling and deteriorating.
3. The adjustment acquisition mechanism utilizes the cooperation of monomer light sources with different wavelengths for illumination and corresponding silicon photocells to analyze a plurality of specific proteins; the adjustment is simple and convenient, and the acquisition and analysis are more flexible.
4. According to the specific protein analysis method, the lifting clamping and oscillating mechanism clamps and transports the detection cup to the adjusting and collecting mechanism after oscillating for the second time, and detection and analysis of specific protein are carried out on detection liquid in the detection cup; and the single light sources with different wavelengths for illumination are matched with corresponding silicon photocells to analyze a plurality of specific proteins. The specific protein analysis method is suitable for synchronously detecting various specific protein analysis items of different detection solutions, and has high analysis efficiency and wide application range.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a front view of a specific protein analysis apparatus in an embodiment of the present invention;
FIG. 2 is a schematic diagram of a main structure of a specific protein analysis apparatus according to an embodiment of the present invention, in which an upper cover is not shown;
FIG. 3 is a schematic structural view of a rotary incubation mechanism in an embodiment of the present invention;
FIG. 4 is a schematic structural view of the rotary incubation mechanism not accommodating the detection cup according to the embodiment of the present invention;
FIG. 5 is a central cross-sectional view of a rotary incubation mechanism in an embodiment of the invention;
FIG. 6 is a schematic view of a connection structure of a nut seat and a lifting and clamping oscillating mechanism according to an embodiment of the present invention;
FIG. 7 is a cross-sectional view of the adapting table, the oscillating chamber and the clamping table according to the embodiment of the present invention;
FIG. 8 is a schematic structural diagram of an adjusting and collecting mechanism in an embodiment of the present invention;
FIG. 9 is a schematic structural diagram of the adjusting and collecting mechanism with the detection platform removed in the embodiment of the present invention;
FIG. 10 is a side view of a light source board in an embodiment of the invention;
fig. 11 is a side view of a photoelectric collection plate in an embodiment of the present invention.
Reference numerals: 100. a lower box body; 110. detecting the cup; 120. a pneumatic support rod; 200. an upper cover body; 210. a rotating rod; 300. a rotary incubation mechanism; 310. fixing the disc; 311. an L-shaped bracket; 320. rotating the incubation table; 321. an accommodating cavity; 322. a stabilizing rod; 323. a heat conducting pipe; 330. a first speed regulating motor; 331. a motor shaft; 332. a coupling; 333. a rotating shaft; 340. a heat conducting cavity; 341. a heat-conducting medium; 342. a media inlet valve; 343. a media outlet valve; 400. a temporary transportation storage mechanism; 410. a transfer belt; 420. a driving roller; 430. a limiting plate; 500. adjusting the acquisition mechanism; 510. a third speed-regulating motor; 520. a bearing table; 530. a first light shielding plate; 540. a second light shielding plate; 550. a rotating shaft; 560. a light source plate; 561. a first through hole; 562. a single light source; 570. a photoelectric collecting plate; 571. a second through hole; 572. a silicon photocell; 580. a detection table; 581. a detection chamber; 600. a clamping and transferring mechanism; 610. a second speed regulating motor; 620. a ball screw; 630. a nut seat; 640. a lifting clamping oscillation mechanism; 641. a telescopic cylinder; 642. a transfer station; 643. an oscillation chamber; 644. a servo motor; 645. a clamping table; 646. a clamping cavity; 647. a damping spring; 650. and a bearing seat.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1-2, the present embodiment provides a specific protein analysis apparatus, which includes a lower case 100 and an upper cover 200 covering the lower case 100, wherein the lower case 100 is provided with a rotary incubation mechanism 300 for accommodating, rotationally oscillating and incubating a plurality of detection cups 110, the periphery of the rotary incubation mechanism 300 is provided with a transportation temporary storage mechanism 400, one side of the transportation temporary storage mechanism 400 far away from the rotary incubation mechanism 300 is provided with an adjustment collecting mechanism 500, and the inner wall of the upper cover 200 is provided with a clamping and transferring mechanism 600 for clamping and transferring the detection cups 110.
The specific protein analysis equipment of this embodiment, transportation temporary storage mechanism 400, adjust collection mechanism 500, the quantity of centre gripping transport mechanism 600 can be a plurality of and locate the periphery of rotatory incubation mechanism 300, rotatory incubation mechanism 300 carries out the holding to a plurality of detection cups 110, rotatory oscillation and incubation, a plurality of centre gripping transport mechanism 600 carry out the centre gripping simultaneously to a plurality of detection cups 110 and transport, transportation temporary storage mechanism 400 keeps in and transports, centre gripping transport mechanism 600 will detect cup 110 centre gripping in proper order and transport to adjusting collection mechanism 500 department, carry out the detection and analysis of specific protein to the detection liquid that detects in the detection cup 110. The specific protein analysis equipment realizes simultaneous accommodation, rotary oscillation and incubation of a plurality of detection cups 110, transports, detects and collects the plurality of detection cups 110, and is suitable for synchronously detecting a plurality of specific protein analysis items of different detection liquids.
Certainly, the specific protein analysis device mentioned in this embodiment further includes a touch screen, a built-in printer, an RS232 interface, and a data processing and storing structure, and the corresponding structures and principles all belong to the prior art, and are not described herein again. The specific protein analysis equipment is suitable for detecting specific proteins (procalcitonin, beta-microglobulin, C-reactive protein, D-dimer, heart fatty acid binding protein, hypersensitive C-reactive protein, microalbumin, cystatin C, transferrin, retinol binding protein, neutrophil gelatinase-associated carrier protein, rheumatoid factor and streptococcin O) of blood samples and body fluids of patients in a clinical laboratory.
Specifically, as shown in fig. 2-5, the rotary incubation mechanism 300 includes a fixed disk 310 and a rotary incubation table 320, a cavity is formed in the fixed disk 310, the rotary incubation table 320 is slidably disposed in the cavity of the fixed disk 310, a plurality of accommodating cavities 321 for accommodating the detection cups 110 are distributed in an annular array on the upper surface of the rotary incubation table 320, a first speed-regulating motor 330 is disposed at the central bottom of the fixed disk 310, and a motor shaft 331 of the first speed-regulating motor 330 is connected to a rotating shaft 333 penetrating through the fixed disk 310 to the lower surface of the rotary incubation table 320 through a coupling 332. The inner cavity of the fixed disk 310 is provided with a heat conducting cavity 340, and a heat conducting medium 341 is introduced into the heat conducting cavity 340. The heat transfer medium 341 is selected from warm water or heat transfer oil.
The arrangement of the rotary incubation mechanism 300 is that after the plurality of detection cups 110 are placed in the accommodating cavity 321, the first speed regulating motor 330 drives the rotary shaft 333 to rotate through the coupler 332, and then the rotary shaft 333 drives the rotary incubation table 320 to rotate, so that synchronous oscillation of detection liquid in the plurality of detection cups 110 is realized, and substances in the detection liquid are fully dispersed; after the heat conducting medium 341 is introduced into the heat conducting chamber 340, the heat conducting medium 341 conducts heat into each accommodating chamber 321 along the rotary incubation table 320, so as to realize synchronous heating and incubation of the detection solutions in the plurality of detection cups 110. The heat conducting area is large due to the heat conducting mode of the heat conducting medium 341, the heat conducting efficiency is high, the heat absorption of the detection cup 110 is uniform, the temperature rise is stable, and the defects that the temperature rise is unstable and the electric energy consumption is high in the traditional electric heating mode are overcome.
The two sides of the bottom of the fixed disc 310 are connected with L-shaped brackets 311, the upper surface of the center of the rotary incubation table 320 is provided with a stabilizing rod 322, and the bottom end of the stabilizing rod 322 is in threaded connection with the top end of the rotating shaft 333; the accommodating cavity 321 is in interference fit with the detection cup 110, and the height of the detection cup 110 is greater than that of the accommodating cavity 321; the bottom of the accommodating chamber 321 is provided with a plurality of heat conduction pipes 323 communicated with the heat conduction chamber 340.
L-shaped bracket 311 supports fixed tray 310, making the rotation process of first adjustable speed motor 330 driving rotary incubation table 320 more stable. After the stabilizing rod 322 is connected with the rotating shaft 333 through the screw thread, the stabilizing rod 322 and the rotating shaft 333 synchronously drive the rotating incubation table 320 to rotate, and the stability in the rotating process is kept. The heat generated by the heat-conducting medium 341 is conducted into the accommodating chamber 321 through the rotating incubation table 320, and is conducted into the accommodating chamber 321 through the plurality of heat-conducting pipes 323, so that the heat entering the accommodating chamber 321 stably heats the detection cup 110.
Wherein, the main body of the rotary incubation table 320 is cylindrical, the accommodating cavity 321 and the detection cup 110 are cylindrical, and the cross section of the heat conducting pipe 323 is wavy; the two sides of the bottom of the heat conducting cavity 340 are respectively provided with a medium inlet valve 342 and a medium outlet valve 343. The wavy heat conduction pipe 323 increases the heat conduction area, improves the heat preservation efficiency, and the medium inlet valve 342 and the medium outlet valve 343 are matched to seal and discharge the heat conduction medium 341.
Example 2
As shown in fig. 2 and 6-7, the plurality of clamping and transferring mechanisms 600 are arranged right above the rotary incubation mechanism 300, the transport temporary storage mechanism 400 and the adjustment and collection mechanism 500, each clamping and transferring mechanism 600 comprises a second speed regulation motor 610, a ball screw 620, a nut seat 630 and a lifting and clamping oscillating mechanism 640, the second speed regulation motor 610 is connected with the ball screw 620 through a coupler, an inner cavity of the nut seat 630 is provided with an inner thread matched with the outer thread of the ball screw 620, the lifting and clamping oscillating mechanism 640 is arranged below the nut seat 630, and the tail end of the ball screw 620 is connected with a bearing seat 650. In the clamping and transferring mechanism 600, when the second speed regulating motor 610 drives the ball screw 620 to rotate, the nut seat 630 engaged with the ball screw 620 moves along the axial direction of the ball screw 620, and the nut seat 630 drives the lifting and clamping oscillating mechanism 640 to move synchronously.
The lifting clamping oscillating mechanism 640 comprises a telescopic cylinder 641, an adapter 642 and an oscillating chamber 643, wherein the telescopic cylinder 641 is arranged below the nut base 630, the adapter 642 is fixedly connected below the telescopic cylinder 641, the oscillating chamber 643 is arranged below the adapter 642, a servo motor 644 is arranged in the oscillating chamber 643, and a motor shaft of the servo motor 644 extends downwards out of the oscillating chamber 643 and then is connected with the clamping table 645.
A clamping cavity 646 is arranged in the clamping table 645, and the inner circumference size of the clamping cavity 646 is matched with the outer circumference size of the detection cup 110. A plurality of damping springs 647 are symmetrically arranged between the housing of the servo motor 644 and the inner wall of the oscillation chamber 643. The damping spring 647 slows down the vibration of the oscillation chamber 643 caused by the operation of the servo motor 644, and avoids disordered and disordered shaking of the detection liquid in the detection cup 110. In order to stably clamp and place the detection cup 110 in the clamping cavity 646, the clamping cavity 646 may be connected to a vacuum absorption structure, specifically, the vacuum absorption structure may include a vacuum pump and a vacuum tube, and the vacuum pump absorbs vacuum in the clamping cavity 646 through the vacuum tube, so as to more stably clamp and place the detection cup 110.
After the telescopic cylinder 641 extends downwards, the clamping cavity 646 in the clamping table 645 clamps the detection cup 110, and in the process that the servo motor 644 drives the clamping table 645 to rotate, the detection liquid in the detection cup 110 is subjected to secondary oscillation mixing, so that the detection liquid is prevented from being settled and deteriorated. The lifting clamping oscillating mechanism 640 moves along the ball screw 620, when the clamping table 645 moves above the transportation temporary storage mechanism 400, the telescopic cylinder 641 extends downwards to place the detection cup 110 on the transportation temporary storage mechanism 400, and when the clamping table 645 moves above the adjustment acquisition mechanism 500, the telescopic cylinder 641 extends downwards to place the detection cup 110 on the adjustment acquisition mechanism 500 to analyze specific proteins. The lifting clamping oscillating mechanism 640 realizes clamping lifting and secondary oscillation mixing of the detection cup 110, and has the effect of preventing the detection liquid from settling and deteriorating.
The temporary transport storage mechanism 400 includes a transmission belt 410 disposed around the conveyor belt 410, a plurality of transmission rollers 420 are disposed in the transmission belt 410 at equal intervals, and one transmission roller 420 at the end is driven to rotate by a driving device, so as to drive the transmission belt 410 to transmit around the plurality of transmission rollers 420. Two sides of the length direction of the conveying belt 410 are provided with ring-shaped limiting plates 430, and the size between the two corresponding limiting plates 430 is adapted to the outer circumference size of the detecting cup 110. The ring-shaped limiting plates 430 arranged on the two sides limit and fix the detection cups 110, and a plurality of detection cups 110 can be transported in the process of transporting the transmission belt 410.
Example 3
As shown in fig. 2 and fig. 8-11, the adjusting and collecting mechanism 500 includes a third adjustable speed motor 510 and a carrier 520, the cross section of the carrier 520 is U-shaped, two sides of the carrier 520 are respectively provided with a first light shielding plate 530 and a second light shielding plate 540, the third adjustable speed motor 510 is connected with a rotation shaft 550 penetrating through the first light shielding plate 530 to the outside of the second light shielding plate 540, the third adjustable speed motor 510 is arranged at the outside of the first light shielding plate 530, the inner wall of the rotation shaft 550 at the periphery of the first light shielding plate 530 is provided with a light source plate 560, and the inner wall of the rotation shaft 550 at the periphery of the second light shielding plate 540 is provided with a photoelectric collecting plate 570. When the third adjustable speed motor 510 is started, the rotating shaft 550 is driven to rotate, and the light source plate 560 and the photoelectric collecting plate 570 rotate along with the rotating shaft, so that the light source plate 560 and the photoelectric collecting plate 570 are linked in a consistent manner.
The first light shielding plate 530 and the second light shielding plate 540 are higher than the carrier 520, a detection platform 580 is disposed on the upper surface of the carrier 520, and a detection cavity 581 for accommodating the detection cup 110 is disposed on the detection platform 580. The center of the light source plate 560 is provided with a first through hole 561 for the rotation shaft 550 to pass through, and a plurality of single light sources 562 emitting light of different wavelengths are distributed on one surface of the light source plate 560 close to the detection platform 580 in an annular array. A second through hole 571 through which the rotating shaft 550 penetrates is formed in the center of the photoelectric acquisition plate 570, and a plurality of silicon photocells 572 are distributed on one surface, close to the detection table 580, of the photoelectric acquisition plate 570 in an annular array manner; the silicon photocells 572 and the single light sources 562 are the same in number and correspond to each other one by one.
The detection cup 110 is provided with a corresponding light inlet and a corresponding light outlet, the single light source 562 corresponds to the light inlet, and the silicon photocell 572 corresponds to the light outlet. After the clamping table 645 places the detection cup 110 in the detection cavity 581 of the detection table 580, through the consistent linkage of the light source plate 560 and the photoelectric acquisition plate 570, light emitted by the monomer light source 562 passes through the light inlet hole on the detection cup 110, and is emitted from the light outlet hole to the corresponding silicon photocell 572, and specific protein is analyzed according to the colorimetric principle; the monomer light sources 562 with different wavelengths and the corresponding silicon photocells 572 are matched to analyze a plurality of specific proteins; the adjustment is simple and convenient, and the acquisition and analysis are more flexible. The first light shielding plate 530 and the second light shielding plate 540 cooperate to prevent the diffuse reflection of illumination, and the accuracy of analysis is guaranteed.
As shown in fig. 1, two sides of the bottom of the upper cover 200 are rotatably connected to the lower case 100 through the rotating rod 210, and two pneumatic support rods 120 for supporting the upper cover 200 are symmetrically disposed on the lower case 100. The pneumatic support rod 120 supports the upper cover 200, and the upper cover 200 can be covered on the lower box 100 after rotating through the rotating rod 210, so as to maintain the sealing performance of the box.
Example 4
As shown in fig. 1 to 11, the present example provides a specific protein analysis method, comprising the steps of:
s1, sample loading and incubation: diluting human blood or urine to be detected by a solvent to obtain a detection liquid, putting the detection liquid into the detection cup 110, and sealing by covering the detection cup with an upper cover; the plurality of detection cups 110 are placed in the accommodating cavities 321, the heat conducting medium 341 is introduced into the heat conducting cavity 340, the heat conducting medium 341 conducts heat into each accommodating cavity 321 along the rotary incubation table 320, and the detection solution in the plurality of detection cups 110 is synchronously heated and incubated.
S2, synchronous oscillation: the first speed regulating motor 330 drives the rotating shaft 333 to rotate through the coupling 332, and the rotating shaft 333 drives the rotating incubator 320 to rotate, so that the detection liquids in the plurality of detection cups 110 synchronously oscillate.
S3, clamping and transferring: the plurality of second speed regulating motors 610 drive the ball screw 620 to rotate, the nut seat 630 meshed with the ball screw 620 axially moves along the ball screw 620, and the nut seat 630 drives the lifting clamping oscillating mechanism 640 to synchronously move; when the lifting and clamping oscillating mechanism 640 moves to the position above the detection cup 110, the telescopic cylinder 641 extends downwards, and the clamping cavity 646 in the clamping table 645 clamps the detection cup 110; when the telescopic cylinder 641 contracts upwards and the nut seat 630 drives the lifting clamping oscillation mechanism 640 to move to the position above the temporary transportation storage mechanism 400, the telescopic cylinder 641 extends downwards, and the clamping table 645 places the detection cup 110 in the annular limiting plate 430; the step S3 is repeated to temporarily store the plurality of detection cups 110 on the conveying belt 410.
S4, secondary oscillation and transfer: the conveying belt 410 is driven around a plurality of driving rollers 420 to convey the inspection cup 110 forward; when the lifting clamping oscillation mechanism 640 moves to the position above the detection cup 110, the telescopic cylinder 641 extends downwards, the clamping cavity 646 in the clamping table 645 clamps the detection cup 110, and the servo motor 644 drives the clamping table 645 to rotate to perform secondary oscillation mixing on the detection liquid in the detection cup 110; the nut seat 630 drives the lifting clamping oscillation mechanism 640 to move above the detection table 580, and the telescopic cylinder 641 extends downwards to place the detection cup 110 in the detection cavity 581;
s5, detection: the third speed regulating motor 510 is started to drive the rotating shaft 550 to rotate, the light source plate 560 and the photoelectric acquisition plate 570 rotate along with the rotating shaft, and the light source plate 560 and the photoelectric acquisition plate 570 are linked in a consistent manner; light emitted by the monomer light source 562 passes through the light inlet hole on the detection cup 110 and is emitted to the corresponding silicon photocell 572 from the light outlet hole, and specific protein is analyzed; the monomer light sources 562 with different wavelengths and the corresponding silicon photocells 572 cooperate to perform analysis of a plurality of specific proteins.
After the detection is finished, the detection cup 110 is clamped by the clamping cavity 646 in the clamping table 645, the nut seat 630 drives the lifting and clamping oscillating mechanism 640 to move to the position above the rotary incubation table 320, and the telescopic cylinder 641 extends downwards to place the detection cup 110 in the accommodating cavity 321. The steps S4-S5 are repeated to perform specific protein analysis on the detection solutions in the plurality of detection cups 110.
The principle of specific protein analysis in this embodiment is an immune-scattering turbidimetry, and specifically, when light with a certain wavelength is irradiated along a horizontal axis and passes through the detection liquid, the light encounters the antigen-antibody complex particles, the light is refracted by the particle particles and is deflected, and the angle of deflection of the light is closely related to the wavelength of the emitted light, the size and the amount of the antigen-antibody complex particles. The intensity of the scattered light is proportional to the amount of complex, i.e., the more antigen to be detected, the stronger the scattered light.
In the specific protein analysis method of this embodiment, after the plurality of detection cups 110 are accommodated, rotationally oscillated and incubated, the plurality of lifting clamping oscillating mechanisms 640 simultaneously clamp and transport the plurality of detection cups 110, after the temporary storage and transportation of the plurality of detection cups 110 are performed by the temporary storage and transportation mechanism 400, the lifting clamping oscillating mechanisms 640 clamp and transport the detection cups 110 to the position of the adjustment and collection mechanism 500 after secondary oscillation, and perform specific protein detection and analysis on the detection solution in the detection cups 110; the monomer light sources 562 with different wavelengths and the corresponding silicon photocells 572 cooperate to perform analysis of a plurality of specific proteins. The specific protein analysis method realizes simultaneous accommodation, rotary oscillation and incubation of a plurality of detection cups 110, performs secondary oscillation before detection and collection of the detection cups 110 to avoid the detection liquid from settling and deteriorating, is suitable for synchronously detecting various specific protein analysis items of different detection liquids, and has high analysis efficiency and wide application range.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the following claims.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (10)
1. A specific protein analysis device comprises a lower box body (100) and an upper cover body (200) covering the lower box body (100), and is characterized in that the lower box body (100) is provided with a rotary incubation mechanism (300) for accommodating, rotationally oscillating and incubating a plurality of detection cups (110), the periphery of the rotary incubation mechanism (300) is provided with a temporary transportation storage mechanism (400), one side of the temporary transportation storage mechanism (400) far away from the rotary incubation mechanism (300) is provided with an adjusting and collecting mechanism (500), and the inner wall of the upper cover body (200) is provided with a clamping and transferring mechanism (600) for clamping and transferring the detection cups (110);
the rotary incubation mechanism (300) comprises a fixed disc (310) and a rotary incubation table (320), a cavity is formed in the fixed disc (310), the rotary incubation table (320) is arranged in the cavity of the fixed disc (310) in a sliding mode, a plurality of accommodating cavities (321) used for accommodating the detection cups (110) are distributed on the upper surface of the rotary incubation table (320) in an annular array mode, a first speed regulating motor (330) is arranged at the bottom of the center of the fixed disc (310), and a motor shaft (331) of the first speed regulating motor (330) is connected with a rotating shaft (333) penetrating through the fixed disc (310) to the lower surface of the rotary incubation table (320) through a coupler (332); the inner cavity of the fixed disc (310) is provided with a heat conduction cavity (340), and a heat conduction medium (341) is introduced into the heat conduction cavity (340).
2. The specific protein analysis apparatus according to claim 1, wherein the fixed tray (310) is connected with L-shaped brackets (311) at two sides of the bottom thereof, the rotary incubation table (320) is provided with a stabilizing rod (322) on the central upper surface thereof, and the bottom end of the stabilizing rod (322) is in threaded connection with the top end of the rotary shaft (333); the accommodating cavity (321) is in interference fit with the detection cup (110), and the height of the detection cup (110) is greater than that of the accommodating cavity (321); the bottom of the containing cavity (321) is provided with a plurality of heat conducting pipes (323) communicated with the heat conducting cavity (340).
3. The specific protein analysis equipment according to claim 1, wherein a plurality of clamping and transferring mechanisms (600) are arranged right above the rotary incubation mechanism (300), the transport temporary storage mechanism (400) and the adjustment and collection mechanism (500), the clamping and transferring mechanisms (600) comprise a second speed regulating motor (610), a ball screw (620), a nut seat (630) and a lifting and clamping oscillating mechanism (640), the second speed regulating motor (610) is connected with the ball screw (620) through a coupler, an inner cavity of the nut seat (630) is provided with an inner thread matched with the outer thread of the ball screw (620), the lifting and clamping oscillating mechanism (640) is arranged below the nut seat (630), and the tail end of the ball screw (620) is connected with a bearing seat (650).
4. The specific protein analysis equipment according to claim 3, wherein the lifting clamping oscillation mechanism (640) comprises a telescopic cylinder (641), an adapter table (642) and an oscillation chamber (643), the telescopic cylinder (641) is arranged below the nut seat (630), the adapter table (642) is fixedly connected below the telescopic cylinder (641), the oscillation chamber (643) is arranged below the adapter table (642), a servo motor (644) is arranged in the oscillation chamber (643), and a motor shaft of the servo motor (644) extends downwards out of the oscillation chamber (643) and then is connected with the clamping table (645).
5. The specific protein analysis apparatus according to claim 4, wherein a holding chamber (646) is provided in the holding stage (645), and an inner dimension of the holding chamber (646) is adapted to an outer dimension of the detection cup (110); a plurality of damping springs (647) are symmetrically arranged between the shell of the servo motor (644) and the inner wall of the oscillation chamber (643).
6. The specific protein analysis equipment according to claim 1, wherein the transportation temporary storage mechanism (400) comprises a transmission belt (410) which is arranged in a surrounding way, a plurality of transmission rollers (420) are arranged in the transmission belt (410) at equal intervals, and one transmission roller (420) at the end is driven to rotate by a driving device; two sides of the length direction of the conveying belt (410) are provided with annular limiting plates (430), and the size between the two corresponding limiting plates (430) is matched with the peripheral size of the detection cup (110).
7. The specific protein analysis apparatus according to claim 1, wherein the adjustment and collection mechanism (500) comprises a third speed-adjustable motor (510) and a carrier (520), the carrier (520) has a U-shaped cross section, a first light shielding plate (530) and a second light shielding plate (540) are respectively disposed on two sides of the carrier (520), the third speed-adjustable motor (510) is connected with a rotation shaft (550) passing through the first light shielding plate (530) to the outer side of the second light shielding plate (540), the third speed-adjustable motor (510) is disposed on the outer side of the first light shielding plate (530), a light source plate (560) is disposed on the inner wall of the first light shielding plate (530) on the periphery of the rotation shaft (550), and a photoelectric collection plate (570) is disposed on the inner wall of the second light shielding plate (540) on the periphery of the rotation shaft (550).
8. The specific protein analysis apparatus according to claim 7, wherein the first light shielding plate (530) and the second light shielding plate (540) have a height higher than that of the carrier (520), the upper surface of the carrier (520) is provided with a detection table (580), and the detection table (580) is provided with a detection cavity (581) for accommodating the detection cup (110); a first through hole (561) for the rotating shaft (550) to penetrate through is formed in the center of the light source plate (560), and a plurality of single light sources (562) emitting light with different wavelengths are distributed on one surface, close to the detection table (580), of the light source plate (560) in an annular array mode; a second through hole (571) for the rotating shaft (550) to penetrate through is formed in the center of the photoelectric acquisition plate (570), and a plurality of silicon photocells (572) are distributed on one surface, close to the detection table (580), of the photoelectric acquisition plate (570) in an annular array manner; the number of the silicon photocells (572) is the same as that of the single light sources (562), and the silicon photocells and the single light sources are in one-to-one correspondence.
9. A method for analyzing a specific protein, comprising the steps of:
s1, sample loading and incubation: diluting human blood or urine to be detected by a solvent to obtain a detection liquid, putting the detection liquid into a detection cup (110), and covering and sealing the detection cup by an upper cover; the detection cups (110) are placed in the accommodating cavities (321), heat conducting media (341) are introduced into the heat conducting cavities (340), the heat conducting media (341) conduct heat into the accommodating cavities (321) along the rotary incubation table (320), and the detection liquid in the detection cups (110) is synchronously heated and incubated;
s2, synchronous oscillation: the first speed regulating motor (330) drives the rotating shaft (333) to rotate through the coupler (332), and the rotating shaft (333) drives the rotating incubation table (320) to rotate, so that detection liquid in the detection cups (110) synchronously oscillates;
s3, clamping and transferring: the second speed regulating motors (610) drive the ball screw (620) to rotate, the nut seat (630) meshed with the ball screw (620) axially moves along the ball screw (620), and the nut seat (630) drives the lifting clamping oscillating mechanism (640) to synchronously move; when the lifting clamping oscillating mechanism (640) moves to the position above the detection cup (110), the telescopic cylinder (641) extends downwards, and the clamping cavity (646) in the clamping table (645) clamps the detection cup (110); when the telescopic cylinder (641) contracts upwards, the nut seat (630) drives the lifting clamping oscillating mechanism (640) to move to the upper part of the temporary transportation storage mechanism (400), the telescopic cylinder (641) extends downwards, and the clamping table (645) places the detection cup (110) in the annular limiting plate (430); repeating the step S3, and temporarily storing a plurality of detection cups (110) on the conveying belt (410);
s4, secondary oscillation and transfer: the transmission belt (410) is transmitted around a plurality of transmission rollers (420) and transmits the detection cup (110) forwards; when the lifting clamping oscillating mechanism (640) moves to the position above the detection cup (110), the telescopic cylinder (641) extends downwards, the clamping cavity (646) in the clamping table (645) clamps the detection cup (110), and the servo motor (644) drives the clamping table (645) to rotate to perform secondary oscillation mixing on detection liquid in the detection cup (110); the nut seat (630) drives the lifting clamping oscillation mechanism (640) to move above the detection table (580), and the telescopic cylinder (641) extends downwards to place the detection cup (110) in the detection cavity (581);
s5, detection: starting a third speed regulating motor (510) to drive a rotating shaft (550) to rotate, rotating a light source plate (560) and a photoelectric acquisition plate (570) along with the rotation, and linking the light source plate (560) and the photoelectric acquisition plate (570) in a consistent manner; light emitted by the monomer light source (562) passes through the light inlet hole on the detection cup (110) and is emitted to the corresponding silicon photocell (572) from the light outlet hole, and specific protein is analyzed; the single light sources (562) with different wavelengths of light are matched with the corresponding silicon photocells (572) to analyze a plurality of specific proteins.
10. The specific protein analysis method according to claim 9, wherein after the detection, the detection cup (110) is clamped by the clamping cavity (646) in the clamping table (645), the nut seat (630) drives the lifting clamping oscillation mechanism (640) to move above the rotary incubation table (320), and the telescopic cylinder (641) extends downwards to place the detection cup (110) in the accommodating cavity (321); repeating the steps S4-S5, and performing specific protein analysis on the detection solution in the plurality of detection cups (110).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111178411.6A CN113865962A (en) | 2021-10-10 | 2021-10-10 | Specific protein analysis equipment and method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111178411.6A CN113865962A (en) | 2021-10-10 | 2021-10-10 | Specific protein analysis equipment and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117192142A (en) * | 2023-11-08 | 2023-12-08 | 牡丹江国际旅行卫生保健中心(牡丹江海关口岸门诊部) | Full-automatic blood analyzer convenient for storing blood sample |
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2021
- 2021-10-10 CN CN202111178411.6A patent/CN113865962A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117192142A (en) * | 2023-11-08 | 2023-12-08 | 牡丹江国际旅行卫生保健中心(牡丹江海关口岸门诊部) | Full-automatic blood analyzer convenient for storing blood sample |
| CN117192142B (en) * | 2023-11-08 | 2024-02-09 | 牡丹江国际旅行卫生保健中心(牡丹江海关口岸门诊部) | Full-automatic blood analyzer convenient for storing blood sample |
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