CN116754335B - Sample pretreatment device applied to immunochromatography - Google Patents
Sample pretreatment device applied to immunochromatography Download PDFInfo
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- CN116754335B CN116754335B CN202311053922.4A CN202311053922A CN116754335B CN 116754335 B CN116754335 B CN 116754335B CN 202311053922 A CN202311053922 A CN 202311053922A CN 116754335 B CN116754335 B CN 116754335B
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- 238000003317 immunochromatography Methods 0.000 title claims abstract description 27
- 230000005540 biological transmission Effects 0.000 claims abstract description 24
- 238000000227 grinding Methods 0.000 claims abstract description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 17
- 238000012856 packing Methods 0.000 claims description 20
- 230000005484 gravity Effects 0.000 claims description 9
- 230000013011 mating Effects 0.000 claims description 6
- 230000003068 static effect Effects 0.000 claims description 3
- 210000004209 hair Anatomy 0.000 abstract description 32
- 239000003814 drug Substances 0.000 abstract description 20
- 229940079593 drug Drugs 0.000 abstract description 20
- 230000033001 locomotion Effects 0.000 abstract description 7
- 239000011324 bead Substances 0.000 abstract description 5
- 238000007689 inspection Methods 0.000 abstract description 2
- 239000002131 composite material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 5
- 231100000640 hair analysis Toxicity 0.000 description 5
- 210000001124 body fluid Anatomy 0.000 description 4
- 239000010839 body fluid Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000006166 lysate Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229910052845 zircon Inorganic materials 0.000 description 1
- GFQYVLUOOAAOGM-UHFFFAOYSA-N zirconium(iv) silicate Chemical compound [Zr+4].[O-][Si]([O-])([O-])[O-] GFQYVLUOOAAOGM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of sample inspection and discloses a sample pretreatment device applied to immunochromatography, which comprises a bracket, a treatment component and a storage component, wherein the storage component is used for placing a sample piece, the storage component is arranged on the bottom end of the treatment component, and the treatment component is arranged on the top end of the bracket; the processing assembly comprises a double-shaft motor, a counterweight unit, a ball head rod and a fixing seat, wherein the top end of the ball head rod is movably connected with the inside of the fixing seat. According to the invention, the eccentric block drives the sample tube to shake and collide with the grinding beads to grind, so that a composite motion of radial swing and conical swing is formed, meanwhile, the sample tube can horizontally rotate, the sample tube is processed by the processing assembly, the hair processing time can be obviously shortened, drugs in hair can be rapidly and stably released, the reagents are dripped into the sample tube at intervals by utilizing the transmission structure, manual intervention is not needed, and a plurality of operation steps such as grinding, dissolving and centrifuging can be stably completed by deforming the eccentric block, and repeated transfer of different equipment operations is not needed.
Description
Technical Field
The invention relates to the field of sample inspection, in particular to a sample pretreatment device applied to immunochromatography.
Background
At present, body fluid (mainly urine and blood) is still a biological detection material for detecting drugs, but the detection time limit of drugs in the body fluid is short, the detection material is not easy to store, and only information of short-term drug use of individuals can be provided; the hair is easy to obtain materials, convenient to carry, easy to store for a long time and long in which drugs are metabolized slowly, so that the hair analysis is more practical and convenient than the body fluid analysis in the aspect of identifying drugs.
A pretreatment method for detecting drugs in human hair as proposed in application publication No. CN 110702479A, and this application document proposes a method for determining the drug content in hair by immunochromatography, wherein the pretreatment operation requires multiple steps of operations such as grinding, dissolving, and the like, multiple machines are replaced, and meanwhile, the operation effects such as grinding, and the like in the pretreatment process of hair are poor, and the error of the drug content identified by a sample tube is large, which affects the judgment of the identification result.
Disclosure of Invention
The invention provides a sample pretreatment device applied to an immunochromatography, which solves the technical problems of low efficiency and long process of the prior art that a plurality of devices are required to complete pretreatment operation in a matched mode.
The sample pretreatment device comprises a bracket, a treatment component and a storage component, wherein the storage component is used for placing a sample piece, the storage component is arranged on the bottom end of the treatment component, and the treatment component is arranged on the top end of the bracket;
the processing assembly comprises a double-shaft motor, a counterweight unit, a ball head rod and a fixed seat, wherein the top end of the ball head rod is movably connected in the fixed seat, the bottom end of the ball head rod is connected with the double-shaft motor, output shafts on two sides of the double-shaft motor are respectively connected with the counterweight unit, the counterweight unit comprises an arc-shaped strip and an eccentric block, the eccentric block is of a semicircular structure, a chute is arranged on the inner side of the eccentric block, the chute is semicircular, the arc-shaped strip moves along the chute direction of the chute, when the arc-shaped strip slides in the chute, the gravity center position of the eccentric block can be adjusted, and when the double-shaft motor drives the eccentric blocks on two sides to rotate, the double-shaft motor and the counterweight unit rotate around the joint of the ball head rod and the fixed seat or are relatively static;
the storage component comprises a sample tube and a blanking disc, the blanking disc is arranged below the processing component, the sample tube is arranged on the blanking disc, the sample tube is filled with a sample piece and a grinding piece, and the storage component moves along with the processing component to crush the sample piece through the grinding piece.
Further: the eccentric block is internally provided with a matched gear, a driving frame and an internal tooth column, the internal tooth column is arranged at the joint of the eccentric block and the double-shaft motor, the internal tooth column is in transmission connection with the matched gear, the end part of the driving frame is connected with the middle part of the matched gear, and the driving frame drives the matched gear to move along the groove of the sliding groove.
Further: the inner side of the arc-shaped strip is provided with tooth grooves, and the tooth groove shape of the arc-shaped strip is matched with teeth of the matched gear.
Further: a tensioning spring is arranged between one end of the arc-shaped strip and the groove wall of one end of the sliding groove, the tensioning spring stretches along the groove direction of the sliding groove, and the tensioning spring provides restoring force for the arc-shaped strip to move along the groove direction of the sliding groove.
Further: when the arc-shaped strip rotates 180 degrees along the groove direction of the sliding groove, the weight of the arc-shaped strip is the same as that of the eccentric block, and the gravity centers of the counterweight units on the two sides are both positioned at the connection position of the eccentric block and the output shaft of the double-shaft motor.
Further: the output shaft of the double-shaft motor is also provided with a transmission unit, the bottom end of the transmission unit is connected with the blanking disc, and the transmission unit is used for driving the blanking disc to horizontally rotate.
Further: the transmission unit comprises a rotating disc, a driven gear, a driving gear and a meshing gear, wherein the rotating disc is horizontally arranged above the blanking disc, the driving gear is arranged on an output shaft of the double-shaft motor, the driven gear is connected with the meshing gear through a rod, the driven gear is meshed with the driving gear, and the meshing gear is in transmission connection with the outer edge of the rotating disc.
Further: the upper part of the blanking disc is provided with a filling unit, a filling box is arranged on the filling unit, a reagent is arranged in the filling box, a one-way valve is arranged on the inner wall of the filling box, a convex column wheel is arranged at the tail end of a rod body connected with the meshing gear, a convex part is arranged on one side of the convex column wheel, the convex part abuts against the one-way valve at intervals when the convex column wheel rotates along with the meshing gear, and the filling box is opened to release the reagent into the sample tube.
Further: the lower terminal surface of fixing base is installed and is placed in middle the unit, and the unit of placing in middle is used for driving the bulb pole and adjusts to plumb.
Further: the centering unit comprises an inner gear ring, a driving half gear, a driven half gear and an occlusion frame, wherein the occlusion frame is of an arc-shaped structure, one side of the occlusion frame, which is provided with the driven half gear, is in meshed connection with the inner side teeth of the inner gear ring, and the teeth of the driving half gear are also in meshed connection with the inner side teeth of the inner gear ring.
Further: the inside wall of the occluding frame is provided with a clamping block, and the inner side surface of the clamping block is of a spherical structure.
The invention has the beneficial effects that:
the device can drive the sample tube to shake through the eccentric block and collide with the grinding beads to grind, so that a compound motion of radial swing and conical swing is formed, meanwhile, the sample tube can horizontally rotate, the sample tube is processed through the processing component, the hair processing time can be obviously shortened, drugs in hair can be rapidly and stably released, the purpose that drugs in hair can be rapidly detected is achieved, the stability and accuracy of drug detection in hair are improved, meanwhile, a transmission structure is utilized to drop reagents at intervals, manual intervention is not needed, the eccentric block deforms, multiple operation steps such as grinding, dissolution and centrifugation can be stably completed, operation of different equipment is not needed to be repeatedly transferred, fluorescent immunochromatography or colloidal gold immunochromatography analysis is adopted, compared with the existing hair splitting liquid, the release rate of small molecules of drugs is improved by 5-6 times, and the splitting efficiency is greatly improved.
Drawings
FIG. 1 is a schematic diagram showing a sample pretreatment apparatus for immunochromatography according to the present invention;
FIG. 2 is a front view of FIG. 1;
FIG. 3 shows a processing unit for a sample pretreatment apparatus for immunochromatography according to the present invention;
FIG. 4 is a side view of FIG. 3;
FIG. 5 is a front view of the arcuate strips of FIG. 4 retracted into the eccentric mass;
FIG. 6 is a schematic diagram showing a connection structure of a counterweight unit and a transmission unit applied to a sample pretreatment apparatus for immunochromatography according to the present invention;
FIG. 7 is a schematic view showing the vertical cross-section of an eccentric block applied to a sample pretreatment apparatus for immunochromatography;
FIG. 8 is a schematic diagram showing the structure of a packing unit applied to a sample pretreatment apparatus for immunochromatography according to the present invention;
fig. 9 is a schematic diagram showing a centering unit structure of a sample pretreatment apparatus for immunochromatography according to the present invention.
In the figure: 100. a bracket; 200. a processing assembly; 210. a housing; 220. a filler unit; 221. a drawing plate; 222. a combining seat; 223. a stuffing box; 230. a counterweight unit; 231. an eccentric block; 232. an arc-shaped strip; 233. an inner column; 234. a mating gear; 235. tensioning the spring; 236. a limit column; 237. a limit groove; 238. a drive rack; 240. a fixing seat; 250. a centering unit; 251. a toothed ring; 252. a drive half-gear; 253. a driven half gear; 254. an articulator; 255. clamping blocks; 260. a ball head rod; 270. a biaxial motor; 280. a transmission unit; 281. a rotating disc; 282. a driven gear; 283. a drive gear; 284. a meshing gear; 285. a convex column wheel; 300. a storage component; 310. an outer shield; 320. a sample tube; 330. and (5) blanking a tray.
Detailed Description
The subject matter described herein will now be discussed with reference to example embodiments. It is to be understood that these embodiments are merely discussed so that those skilled in the art may better understand and implement the subject matter described herein and that changes may be made in the function and arrangement of the elements discussed without departing from the scope of the disclosure herein. Various examples may omit, replace, or add various procedures or components as desired. In addition, features described with respect to some examples may be combined in other examples as well.
Referring to fig. 1 to 9, a sample pretreatment apparatus for immunochromatography comprises a holder 100, a treatment member 200, and a storage member 300, wherein the storage member 300 is used for holding a sample member, the storage member 300 is mounted on the bottom end of the treatment member 200, and the treatment member 200 is mounted on the top end of the holder 100;
the processing assembly 200 comprises a double-shaft motor 270, a counterweight unit 230, a ball rod 260 and a fixed seat 240, wherein the top end of the ball rod 260 is movably connected in the fixed seat 240, the bottom end of the ball rod 260 is connected with the double-shaft motor 270, output shafts on two sides of the double-shaft motor 270 are respectively connected with the counterweight unit 230, the counterweight unit 230 comprises an arc-shaped strip 232 and an eccentric block 231, the eccentric block 231 is in a semicircular structure, a chute is arranged on the inner side of the eccentric block 231, the chute is semicircular, the arc-shaped strip 232 moves along the chute direction of the chute, when the arc-shaped strip 232 slides in the chute, the gravity center position of the eccentric block 231 can be adjusted, and when the double-shaft motor 270 drives the eccentric blocks 231 on two sides to rotate, the double-shaft motor 270 and the counterweight unit 230 rotate around the joint of the ball rod 260 and the fixed seat 240 or are relatively static;
a housing 210 is provided at the outer side of the dual-shaft motor 270, and a mounting groove is provided at the middle of the housing 210 for mounting the packing unit 220;
the inner side of the arc-shaped strip 232 is provided with a tooth slot, the tooth slot shape of the arc-shaped strip 232 is matched with the tooth teeth of the matched gear 234, a tensioning spring 235 is arranged between one end of the arc-shaped strip 232 and one end slot wall of the chute, the tensioning spring 235 stretches along the slot direction of the chute, the tensioning spring 235 provides restoring force for the arc-shaped strip 232 to move along the slot direction of the chute, when the arc-shaped strip 232 rotates 180 degrees along the slot direction of the chute, the weight of the arc-shaped strip 232 is identical with that of the eccentric block 231, and the gravity centers of the counterweight units 230 on the two sides are all located at the output shaft connection positions of the eccentric block 231 and the double-shaft motor 270.
The one end that arc strip 232 is close to tensioning spring 235 is equipped with spacing post 236, still is equipped with spacing post 236 in the cell wall of spout, and the tip of spacing post 236 is supported and is connected in spacing groove 237, makes arc strip 232 pass through the cooperation of spacing post 236 and spacing groove 237, makes along the groove to sliding, and does not support the cell wall to the spout.
Meanwhile, a matching gear 234, a driving frame 238 and an internal tooth column 233 are arranged in the eccentric block 231, the internal tooth column 233 is arranged at the joint of the eccentric block 231 and the double-shaft motor 270, the internal tooth column 233 is in transmission connection with the matching gear 234, the end part of the driving frame 238 is connected with the middle part of the matching gear 234, and the driving frame 238 drives the matching gear 234 to move along the groove direction of the sliding groove.
The driving frame 238 is of a Y-shaped structure, the driving frame 238 comprises a driving rod and a driven rod, the rod ends of the driving rod are connected with the driven rods on two sides through shafts, the rod ends of the driven rods are connected with the matched gears 234, driving pieces are arranged on the rod ends of the driving rod, and the driving pieces can be independent power sources and comprise linear moving pieces such as air cylinders.
The storage assembly 300 comprises a sample tube 320 and a blanking disc 330, the blanking disc 330 is arranged below the processing assembly 200, the sample tube 320 is arranged on the blanking disc 330, the sample tube 320 is filled with sample pieces and grinding pieces, and the storage assembly 300 moves along with the processing assembly 200 to crush the sample pieces through the grinding pieces.
Wherein the lower end of the blanking tray 330 is provided with a plurality of blanking connectors, and the inner wall of the opening end of the sample tube 320 is connected with the blanking connectors through threads.
Meanwhile, a fixing groove is formed at the bottom end of the housing 210, an outer shield 310 is inserted into the fixing groove, and the outer shield 310 is sleeved on the outer side of the sample tube 320.
The output shaft of the double-shaft motor 270 is further provided with a transmission unit 280, the bottom end of the transmission unit 280 is connected with the blanking disc 330, the transmission unit 280 is used for driving the blanking disc 330 to horizontally rotate, the moving unit comprises a rotating disc 281, a driven gear 282, a driving gear 283 and a meshing gear 284, the rotating disc 281 is horizontally arranged above the blanking disc 330, the driving gear 283 is arranged on the output shaft of the double-shaft motor 270, the driven gear 282 is connected with the meshing gear 284 through a rod, the driven gear 282 is in meshing connection with the driving gear 283, and the meshing gear 284 is in transmission connection with the outer edge of the rotating disc 281.
The upper part of the blanking disc 330 is provided with a packing unit 220, the packing unit 220 is provided with a packing box 223, a reagent is arranged in the packing box 223, the inner wall of the packing box 223 is provided with a one-way valve, the tail end of a rod body connected with a meshing gear 284 is provided with a convex column wheel 285, one side of the convex column wheel 285 is provided with a convex part, the convex part abuts against the one-way valve at intervals when the convex column wheel 285 rotates along with the meshing gear 284, and the packing box 223 is opened to release the reagent into the sample tube 320.
The packing unit 220 further comprises a drawing plate 221 and a combining seat 222, the drawing plate 221 is horizontally inserted into the mounting groove of the shell 210, the combining seat 222 is located in the middle of the drawing plate 221, the lower end face of the combining seat 222 is combined with the upper end face of the blanking disc 330, packing boxes 223 are annularly distributed above the combining seat 222, and the number of the packing boxes 223 is consistent with the number of blanking connectors on the blanking disc 330.
The centering unit 250 is installed to the lower terminal surface of fixing base 240, centering unit 250 is used for driving ball head pole 260 to adjust to plumb, wherein centering unit 250 includes ring gear 251, drive semi-gear 252, driven semi-gear 253 and interlock frame 254, and interlock frame 254 is the arc structure, and interlock frame 254 is equipped with one side of driven semi-gear 253, and the tooth of driven semi-gear 253 is connected with the inboard tooth of ring gear 251 in the meshing, and the tooth of drive semi-gear 252 is also connected with the inboard tooth of ring gear 251 in the meshing, and clamp block 255 is installed to the inside wall of interlock frame 254, and the medial surface of clamp block 255 is the sphere structure.
The sample pretreatment device is used for hair analysis, and the hair analysis is more practical and convenient than body fluid analysis in identifying drugs, and common hair digestion methods include alkaline hydrolysis, acid hydrolysis, enzyme hydrolysis and the like. The extraction method comprises solid phase extraction, liquid-liquid extraction, supercritical fluid extraction and the like, or adopts fluorescent immunochromatography or colloidal gold immunochromatography for identification, hair needs to be pretreated, the existing pretreatment equipment is used for replacing a plurality of machines through multi-step operations such as grinding, dissolving and the like, meanwhile, the operation effects of grinding and the like in the pretreatment process of the hair are poor, the error of the drug content identified by the sample tube 320 is large, and the judgment of the identification result is influenced.
The sample pretreatment device can drive the sample tube 320 to shake through the eccentric block 231 and collide with the grinding beads for grinding, so that the hair treatment time can be obviously shortened, meanwhile, drugs in the hair can be rapidly and stably released, the purpose of rapidly detecting the drugs in the hair is achieved, the stability and accuracy of drug detection in the hair are improved, meanwhile, the steps of grinding, dissolving, centrifuging and the like can be stably completed by utilizing a transmission structure to drop reagents at intervals without manual intervention, the operation of repeatedly transferring different equipment is not needed, and fluorescent immunochromatography or colloidal gold immunochromatography analysis is adopted, so that compared with the existing hair lysate, the release rate of small molecules of the drugs is improved by 5-6 times, and the cleavage efficiency is greatly improved;
the specific sample pretreatment flow of the device is as follows:
cutting a hair sample into small sections of 2-3cm, filling the small sections into a sample tube 320, putting a proper amount of grinding beads which are zircon and quartz sand commonly used in the prior art into the sample tube 320, fixing the sample tube 320 on a blanking connector of a blanking disc 330, fastening an outer shield 310, standing a bracket 100 on a horizontal workbench, suspending an integral treatment assembly 200, wherein the treatment assembly 200 is in a free state and faces vertically downwards;
then, the required reagent is the reagent required by hair analysis, such as hair lysate, protease and the like, which are all in the prior art and are used for hair cleavage analysis, the reagent is dripped into the stuffing box 223, the reagent amount and the hair amount are filled in proportion, and then the stuffing box is inserted into the mounting groove through the drawing plate 221, so that the pretreatment operation of a sample is started;
when the double-shaft motor 270 drives the eccentric blocks 231 on two sides to rotate, the arc-shaped strips 232 in the eccentric blocks 231 are completely retracted into the sliding grooves, and the eccentric blocks 231 on two sides are arranged as shown in fig. 5, so that when the double-shaft motor 270 drives the eccentric blocks 231 on two sides to rotate, the eccentric blocks 231 are not on the same horizontal line due to the fact that the positions of the centers of gravity on the two sides are not on the same horizontal line, the whole processing assembly 200 rotates around the ball head of the ball head rod 260, the rotation amplitude is controlled by the speed of the double-shaft motor 270, and the processing assembly 200 is driven to do radial oscillation and conical swing compound motion, so that hairs are continuously collided with grinding balls in the sample tube 320 and extruded and ground into small particles;
meanwhile, when the processing assembly 200 moves in a combined mode, the transmission unit 280 drives the blanking disc 330 and the sample tube 320 on the blanking disc to horizontally rotate, and further the grinding motion between the hair and the grinding beads in the sample tube 320 is compounded, so that the granularity of the hair is smaller, drug small molecules are easier to release when hair lysate is added subsequently, wherein the transmission unit 280 acts as follows, the driven gear 282 is driven to rotate through the rotation of the driving gear 283, the meshing gear 284 coaxially rotates along with the driven gear, teeth of the meshing gear 284 are combined with teeth on the rotating disc 281, so that the rotating disc 281 horizontally rotates, and the sample tube 320 is driven to rotate;
after the timing, the hair is ground into small particles, the machine is stopped, the ball head rod 260 is regulated to the vertical direction through the centering unit 250, then the packing unit 220 is inserted into the mounting groove, the reagent on the ball head rod is the hair splitting agent and the protease at the moment, the machine is started again after the insertion, in the rotating process, the reagent is continuously released through the bottom end of the packing box 223 by the convex column wheel 285 continuously abutting against the unidirectional blanking valve in the packing box 223, the reagent enters the sample tube 320 through the blanking connector, the movement at the moment shakes the reagent and the ground hair through the compound movement, the machine is stopped, the machine is regulated to the vertical direction through the centering unit 250 again, the driving frame 238 of the counterweight unit 230 is started at the moment, the driving frame 238 combines the matched gear 234 between the internal tooth column 233 and the arc-shaped strip 232, then the double-shaft motor 270 acts to drive the internal tooth column 233 to rotate to drive the matched gear 234 to rotate, the arc-shaped strip 232 connected in a meshed manner slides out through a chute and rotates 180 degrees, the gravity center of the whole eccentric block 231 and the output shaft of the double-shaft motor 270 are positioned on the same axis, at the moment, the gravity centers of the eccentric blocks 231 on two sides are positioned on the same horizontal line, when the double-shaft motor 270 drives, the eccentric blocks 231 on two sides stably rotate, the processing assembly 200 is not driven to do radial oscillation and cone swing compound movement, but the blanking disc 330 is driven to rotate horizontally to drive the sample tube 320 to centrifuge, so that the split between the hair splitting agent and the hair is layered under the centrifugal action;
it should be added that, the centering unit 250 drives the driving half gear 252 to rotate through the driving piece, drives the toothed ring 251 to rotate, drives the driven half gear 253 to rotate, and the engagement frame 254 on the driven half gear 253 retracts to engage, so that the clamping block 255 abuts against the ball head of the ball head rod 260, and then corrects the ball head rod 260 to be vertical.
And then stopping the machine, centering the ball head rod 260 by the centering unit 250, replacing the new filling unit 220 at the moment, filling the protease and the colloid Jin Rongji into the upper filling box 223, starting the machine, and stopping the machine to take down the sample tube 320 for immunochromatography after the horizontal rotation and uniform mixing, so as to obtain the result of the content of the hair poisoning products.
The embodiment has been described above with reference to the embodiment, but the embodiment is not limited to the above-described specific implementation, which is only illustrative and not restrictive, and many forms can be made by those of ordinary skill in the art, given the benefit of this disclosure, are within the scope of this embodiment.
Claims (7)
1. A sample pretreatment device applied to immunochromatography, comprising a bracket (100), a treatment component (200) and a storage component (300), wherein the storage component (300) is used for placing a sample piece, the storage component (300) is mounted on the bottom end of the treatment component (200), and the treatment component (200) is mounted on the top end of the bracket (100);
the processing assembly (200) comprises a double-shaft motor (270), a counterweight unit (230), a ball rod (260) and a fixed seat (240), wherein the top end of the ball rod (260) is movably connected inside the fixed seat (240), the bottom end of the ball rod (260) is connected with the double-shaft motor (270), output shafts on two sides of the double-shaft motor (270) are respectively connected with the counterweight unit (230), the counterweight unit (230) comprises an arc-shaped strip (232) and an eccentric block (231), the eccentric block (231) is in a semicircular structure, a chute is arranged on the inner side of the eccentric block (231), the chute is semicircular, the arc-shaped strip (232) moves along the chute, when the arc-shaped strip (232) slides in the chute, the gravity center position of the eccentric block (231) can be adjusted, and when the double-shaft motor (270) drives the eccentric blocks (231) on two sides to rotate, the double-shaft motor (270) and the counterweight unit (230) rotate or are relatively static around the joint of the ball rod (260) and the fixed seat (240);
the storage assembly (300) comprises a sample tube (320) and a blanking disc (330), the blanking disc (330) is arranged below the processing assembly (200), the sample tube (320) is arranged on the blanking disc (330), the sample tube (320) is filled with sample pieces and grinding pieces, and the storage assembly (300) moves along with the processing assembly (200) to grind the sample pieces through the grinding pieces;
the output shaft of the double-shaft motor (270) is also provided with a transmission unit (280), the bottom end of the transmission unit (280) is connected with the blanking disc (330), and the transmission unit (280) is used for driving the blanking disc (330) to horizontally rotate;
the transmission unit (280) comprises a rotating disc (281), a driven gear (282), a driving gear (283) and a meshing gear (284), wherein the rotating disc (281) is horizontally arranged above the blanking disc (330), the driving gear (283) is arranged on an output shaft of the double-shaft motor (270), the driven gear (282) is connected with the meshing gear (284) through a rod, the driven gear (282) is in meshing connection with the driving gear (283), and the meshing gear (284) is in transmission connection with the outer edge of the rotating disc (281);
the top of unloading dish (330) is equipped with packing unit (220), is equipped with packing box (223) on packing unit (220), is equipped with reagent in packing box (223), and the inner wall of packing box (223) is equipped with one-way valve, and the body of rod end that meshing gear (284) are connected is equipped with projection wheel (285), and one side of projection wheel (285) is equipped with the bulge, and the bulge is in projection wheel (285) interval when meshing gear (284) rotate and is supported against one-way valve, opens packing box (223) release reagent in sample pipe (320).
2. The sample pretreatment apparatus for immunochromatography according to claim 1, wherein a mating gear (234), a driving rack (238) and an internal gear column (233) are provided in the eccentric block (231), the internal gear column (233) is installed at the joint of the eccentric block (231) and the biaxial motor (270), the internal gear column (233) is in transmission connection with the mating gear (234), and the end of the driving rack (238) is connected with the middle of the mating gear (234), and the driving rack (238) drives the mating gear (234) to move along the groove direction of the chute.
3. The sample pretreatment apparatus for immunochromatography according to claim 2, wherein the inner side of the arc-shaped strip (232) is provided with tooth grooves, and the tooth groove shape of the arc-shaped strip (232) is adapted to the teeth of the mating gear (234).
4. A sample pretreatment apparatus for immunochromatography according to claim 3, in which a tension spring (235) is provided between one end of the arc-shaped bar (232) and one end wall of the chute, the tension spring (235) is stretched along the chute, and the tension spring (235) provides a restoring force of the arc-shaped bar (232) moving along the chute.
5. The sample pretreatment apparatus for immunochromatography according to claim 4, wherein when the arc-shaped strip (232) is rotated 180 ° along the groove direction of the chute, the weight of the arc-shaped strip (232) is the same as the weight of the eccentric block (231), and the centers of gravity of the weight units (230) on both sides are located at the connection of the eccentric block (231) and the output shaft of the biaxial motor (270).
6. The sample pretreatment apparatus for immunochromatography according to claim 1, wherein a centering unit (250) is mounted on a lower end surface of the holder (240), and the centering unit (250) is configured to drive the knob (260) to adjust to a vertical direction.
7. The sample pretreatment apparatus for immunochromatography according to claim 6, wherein the centering unit (250) comprises an inner gear ring (251), a driving half gear (252), a driven half gear (253) and an engaging frame (254), the engaging frame (254) has an arc-shaped structure, one side of the engaging frame (254) provided with the driven half gear (253), teeth of the driven half gear (253) are engaged with inner teeth of the inner gear ring (251), and teeth of the driving half gear (252) are engaged with inner teeth of the inner gear ring (251).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311053922.4A CN116754335B (en) | 2023-08-21 | 2023-08-21 | Sample pretreatment device applied to immunochromatography |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311053922.4A CN116754335B (en) | 2023-08-21 | 2023-08-21 | Sample pretreatment device applied to immunochromatography |
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| CN116754335A CN116754335A (en) | 2023-09-15 |
| CN116754335B true CN116754335B (en) | 2023-10-24 |
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| CN201488909U (en) * | 2009-08-21 | 2010-05-26 | 霍春生 | Eccentric grinder |
| CN106345570A (en) * | 2016-10-27 | 2017-01-25 | 陕西科技大学 | Vibration grinding method with active impact and rotational grinding of grinding medium |
| CN110102207A (en) * | 2019-04-29 | 2019-08-09 | 安庆市汇智科技咨询服务有限公司 | A kind of food inspection stirring homogeneous device |
| CN211299633U (en) * | 2019-08-30 | 2020-08-21 | 杭州九阳小家电有限公司 | Food preparation machine with low noise |
| CN113687038A (en) * | 2021-09-13 | 2021-11-23 | 安徽华圭芯科实验技术有限公司 | Pesticide active ingredient content detecting system |
| CN114659992A (en) * | 2022-03-23 | 2022-06-24 | 苏州市蓝皓计算机科技有限公司 | Analysis device for food sample nutrient components and use method thereof |
| CN217111734U (en) * | 2021-12-17 | 2022-08-02 | 深圳迎凯生物科技有限公司 | Blending device and sample analyzer |
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2023
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201488909U (en) * | 2009-08-21 | 2010-05-26 | 霍春生 | Eccentric grinder |
| CN106345570A (en) * | 2016-10-27 | 2017-01-25 | 陕西科技大学 | Vibration grinding method with active impact and rotational grinding of grinding medium |
| CN110102207A (en) * | 2019-04-29 | 2019-08-09 | 安庆市汇智科技咨询服务有限公司 | A kind of food inspection stirring homogeneous device |
| CN211299633U (en) * | 2019-08-30 | 2020-08-21 | 杭州九阳小家电有限公司 | Food preparation machine with low noise |
| CN113687038A (en) * | 2021-09-13 | 2021-11-23 | 安徽华圭芯科实验技术有限公司 | Pesticide active ingredient content detecting system |
| CN217111734U (en) * | 2021-12-17 | 2022-08-02 | 深圳迎凯生物科技有限公司 | Blending device and sample analyzer |
| CN114659992A (en) * | 2022-03-23 | 2022-06-24 | 苏州市蓝皓计算机科技有限公司 | Analysis device for food sample nutrient components and use method thereof |
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| CN116754335A (en) | 2023-09-15 |
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