CN116735590A - Automatic loading and fixing detection system for small-sized model animals - Google Patents
Automatic loading and fixing detection system for small-sized model animals Download PDFInfo
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- CN116735590A CN116735590A CN202310682671.XA CN202310682671A CN116735590A CN 116735590 A CN116735590 A CN 116735590A CN 202310682671 A CN202310682671 A CN 202310682671A CN 116735590 A CN116735590 A CN 116735590A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Abstract
The invention provides a small-sized model animal automatic loading and fixing detection system, which utilizes a siphon principle to construct a siphon pipeline by using a soft bristle tubule, realizes the pump-free loading and fixing of animals, and completes the imaging analysis of the animals by combining an imaging analysis unit; by controlling the siphon flow rate, the animal is quickly and automatically fixed under the condition of not carrying out gel embedding or long-time anesthesia, the animal fixing efficiency is effectively improved, and the fixing time is shortened. The detection system is simple in structure, convenient to operate, economical and easy to popularize and apply; the method realizes the automatic loading and fixing detection of small-sized animals in high-throughput, automatic, pump-free and non-microfluidic chip modes, and provides a great potential technical support for further realizing the research and the screening of complex disease treatment medicines and the related research of other small-sized animals.
Description
Technical Field
The invention belongs to the technical field of model animal research, and particularly relates to an automatic loading and fixing detection system for small model animals.
Background
High throughput detection of drug, environmental toxicants, gene mutations and other drug-induced phenotypes is critical for modern biology, medical research and environmental risk assessment. Existing high-throughput cell-based detection techniques, but the results are often not related to animal models or clinical results, more and more people are using small model animals for screening and detection.
For example, zebra fish are widely used as small-scale organisms for in vivo chemical and genetic detection, and zebra fish larvae have the advantages of small volume, optical transparency and rapid growth in aqueous media. Zebra fish models of many human diseases have been developed and zebra fish have many useful mutants, which also facilitate the study of gene function and the recognition of new compound cell targets. Aiming at the control and imaging of small model animals such as zebra fish and the like, the method is an important technology for realizing various researches.
The existing control for small model animals is dependent on manual, low-flux and small-scale research methods, so that human errors are easily introduced; or screening and detection in microwell plates, the in vivo complexity of small model animal models cannot be fully exploited. Therefore, how to realize automatic loading and fixing of small model animals is a key problem of related research.
At present, for automatic loading and fixing of small model animals, a very complex system is usually built by means of devices such as a pneumatic micro valve, a programmable injection pump or a microfluidic chip, and the like, so that the operation is inconvenient, the cost is extremely high, and the small model animals are easily damaged. Therefore, there is a need to develop an effective automatic loading and fixing detection system for small animals for related research of the small animals.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a small model animal automatic loading and fixing detection system, which is characterized in that two containers with liquid level difference are arranged, the two containers are connected by adopting a soft bristle tubule and a transparent bristle tubule, the automatic loading of animals is realized by taking gravity as a driving force by utilizing a siphon principle, and imaging analysis is completed by combining an imaging analysis unit; and the automatic fixation of animals is realized by controlling the siphon flow rate, so that conditions are provided for imaging analysis.
The automatic loading and fixing detection system for the small model animals comprises a loading container, an imaging analysis unit, a receiving container and a detection unit for detecting the positions of the animals;
the level of the liquid level in the loading container is higher than the level of the liquid level in the receiving container;
the imaging area of the imaging analysis unit is provided with a bristle tubule, and two ends of the bristle tubule are respectively connected with the bottom of the loading container and the top of the receiving container through the soft capillary tubule;
at least one soft capillary tube is provided with a flow control element which is in signal connection with the detection unit;
when the detection unit detects that the animal enters the bristle tubule, the flow control element controls the fluid to stop flowing, so that the animal stays for imaging analysis by the imaging analysis unit.
In the detection system, the loading container is used for storing small model animals and culture solution, and the receiving container is used for receiving the culture solution and the animals.
The automatic loading and fixing detection system adopts the soft capillary and the bristle capillary to communicate two containers with liquid level height difference, and a siphon pipeline formed by sequentially connecting the soft capillary, the bristle capillary and the soft capillary is communicated with a loading container and a receiving container to be used as a path for automatically loading, fixing and unloading small model animals, and gravity is used as a driving force to realize the automatic loading of the small model animals.
The siphon principle is as follows:
considering local resistance head loss and on-way resistance head loss, and according to an average flow velocity formula of the siphon flow velocity:
wherein v is the average speed of the siphon flow rate; h is the height difference between the water surface at the inlet end and the water surface at the outlet end of the siphon; g is gravity acceleration; lambda is the coefficient of resistance along the siphon; l is the length of the siphon; d is the inner diameter of the siphon pipe; zeta type i Is the local drag coefficient of the siphon.
In the above formula, g, lambda, L, D and ζ i Is constant in a specific siphon channel, so that H and v have a quadratic relation, namely the water surface at the inlet end (the level of the liquid level in the loading container) and the water surface at the outlet end (the water surface at the outlet end of the siphon can be adjustedThe level of the liquid surface in the container) H, a desired siphon flow velocity v is obtained, i.e., an increase, a decrease, and a reverse flow of the siphon flow velocity are achieved.
In addition, the resistance coefficient of the siphon can be changed by adjusting the inner diameter of the siphon pipe, so that the siphon flow velocity v can be adjusted within a limited range.
Therefore, when the animal is loaded to the bristle tubules, the flow control element is arranged on at least one of the bristle tubules, the flow control element enables the corresponding bristle tubules to be closed, the siphon flow speed is reduced to 0, and the animal is fixed in the bristle tubules, so that the imaging analysis unit can perform imaging analysis.
Preferably, the inner diameter of the hard capillary tube is matched with the body width of the animal, and the inner diameter of the hard capillary tube meets the requirements that the animal can normally load and unload, and the animal can be well fixed in the hard capillary tube when the liquid flow rate is 0.
Preferably, the liquid level difference between the loading container and the receiving container is 200-300 mm, and a proper siphon rate can be obtained.
Preferably, the inner diameter of the soft capillary tube is slightly larger than the inner diameter of the hard capillary tube and smaller than the outer diameter of the bristle tubule. The purpose of setting the inside diameter of the soft capillary tube slightly larger than the inside diameter of the bristle tubules is to enable more fluid loading and unloading of the animal.
Preferably, the connection port of the bristle tubule and the soft capillary tube is subjected to round corner treatment so as to ensure that animals are not scratched in the loading process.
Preferably, the loading container comprises a liquid container and an animal container with a Murphy's dropper-like structure, which are arranged up and down;
the top of the liquid container is opened, and the bottom of the liquid container is connected with the top of the animal container through a soft capillary tube;
the lower part of the animal container is funnel-shaped, and the bottom opening is connected with the bristle tubule through the soft capillary tube.
In the technical scheme, only culture solution is placed in the liquid container and is positioned above the animal container; the top opening of which is open to atmosphere for providing liquid for the loading process. The culture solution and the animals are placed in the animal container, the structure of the animal container is similar to that of a Murphy's dropper, and the lower part of the animal container is funnel-shaped, so that the animals can be prevented from being piled up at the opening at the bottom of the animal container, and the loading is not smooth. The loading container is arranged into an upper part and a lower part, so that the loading efficiency of the system can be improved, and system faults caused by the problems of drying and the like of the container can be effectively avoided. In addition, the animal loading inlet is arranged at the bottom of the animal container, so that the siphon difficulty can be reduced.
Preferably, a limiting member for blocking the advance of the animal is arranged in the bristle tubule. The limiting piece is arranged to assist the animal detection unit and the flow control element, so that the position control and fixation of animals are realized, and the acquired information is more accurate. The stop is only used to block the advance of the animal and does not restrict the passage of fluid.
However, due to the small feature size of the limiting member, limited material, etc., it is difficult to process by using the conventional additive/subtractive manufacturing method. Therefore, it is further preferable that the metal tube is designed and manufactured by winding a metal wire, and the metal wire and the bristle tubule are assembled. The specific operation is as follows:
winding a metal wire on the side wall of the metal tube in a mode of carrying out multiple inner and outer interpenetration between two ends of the stainless steel metal tube to form a limiting piece composed of the metal tube and the metal wire; the main body part of the limiting piece is arranged in the set position of the bristle tubule, one end of the metal wire is hooked on the outlet end (the second end) of the bristle tubule when the bristle tubule is loaded, and then the limiting piece is fixed in the bristle tubule through interference fit of the soft bristle tubule and the bristle tubule.
As a further preference, the first ends of the bristle tubules are connected to the bottom opening of the animal container and the receiving container respectively through a three-way joint and two soft capillaries;
the second ends of the bristle tubules are respectively connected with the bottom of the liquid container and the receiving container through a three-way joint and two soft capillaries;
four soft capillaries connected with the bristle capillaries through three-way connectors are respectively provided with a flow control element, and each flow control element is respectively connected with the detection unit in a signal way.
In the technical scheme, a flow control element on a soft capillary tube, of which the first end is connected with an opening at the bottom of an animal container, is used as a first element, and a flow control element on the soft capillary tube, of which the first end is connected with a receiving container, is used as a fourth element; the flow control element on the flexible capillary tube, which is connected to the bottom of the liquid container at the second end of the bristle capillary tube, is used as the second element, and the flow control element on the flexible capillary tube, which is connected to the receiving container, is used as the third element. When the first element and the third element are opened and the second element and the fourth element are closed, the animals and the liquid flow from the first end of the bristle tubule to the second end (forward flow), the animals do not move any more when the animals are loaded to the limiting piece, the first element and the third element are closed, and the imaging analysis unit performs imaging analysis; after the end, the second element and the fourth element are opened, at the moment, the liquid and the animal flow reversely, and the liquid and the animal flow into the receiving container for recycling, and the unloading of the animal is completed.
As a still further preference, the flow control element is a pinch valve.
As a further preferred aspect, the receiving container comprises a liquid receiver and an animal collector, which are connected by a hose;
the bristle tubules are connected at a first end to an animal collector and at a second end to a liquid receiver.
The receiving container is arranged as a liquid receiver and an animal receiver, so that the tested animals can be collected more conveniently and rapidly.
Preferably, the detection system further comprises a hollow shaft stepping motor, and the outlet end (the second end) of the bristle tubule when loaded is coaxially fixed in the hollow shaft of the stepping motor;
the imaging analysis unit is in signal connection with the stepping motor and controls the stepping motor to drive the bristle tubule to rotate around the shaft.
In the technical scheme, the hollow shaft stepping motor is arranged, and the outlet end of the bristle tubule during loading is coaxially fixed in the hollow shaft, so that the stepping motor can control the bristle tubule to rotate around the shaft, and then the gesture of animals in the bristle tubule can be adjusted. The imaging analysis unit is used for carrying out imaging analysis on animals in the bristle tubules, controlling the stepping motor to drive the bristle tubules to rotate around the shaft according to the requirements, and adjusting the animal posture.
In order to fix the bristle tubule in the hollow shaft, it is further preferable that a soft capillary tube is provided between the second end of the bristle tubule and the three-way joint, the soft capillary tube is provided as a transition hose interference sleeve outside the second end of the bristle tubule, and then the transition hose and the second end of the bristle tubule are integrally fixed inside the hollow shaft. The transition hose is sleeved at the second end of the bristle tubule in an interference manner, and meanwhile, the limit piece is installed and fixed.
Preferably, the bristle tubules are borosilicate thin-walled capillaries.
Preferably, the imaging analysis unit comprises a signal-connected microscopic imaging system and a computer.
Preferably, the detection unit comprises a signal-connected microscopic imaging system and a computer.
Further, to save equipment costs, the imaging analysis unit and the detection unit may share a set of microscopic imaging systems and computers.
As a further preference, the microscopic imaging system is an inverted microscope and a microscopic camera.
Preferably, the detection system further comprises a peristaltic pump, wherein the inlet and the outlet of the peristaltic pump are respectively communicated with the liquid receiver and the liquid container, so that the recycling of the nutrient solution is realized.
As a further preferable mode, a liquid level sensor is arranged on the loading container, and the liquid level sensor is connected with the peristaltic pump in a signal way; the liquid level sensor is used for monitoring the liquid level in the loading container and controlling the peristaltic pump to work according to the requirement so that the liquid level in the loading container is kept at a set level.
Preferably, the detection system further comprises a fixing assembly, wherein the fixing assembly comprises an upper mounting plate and a lower mounting plate, the upper mounting plate is fixed on an objective table of an imaging area of the imaging analysis unit, and the upper mounting plate is connected with the lower mounting plate through bolts; the two ends of the hollow shaft of the stepping motor are respectively rotatably arranged between the upper mounting plate and the lower mounting plate through a second bearing; and motor avoiding openings are distributed at corresponding positions on the upper mounting plate and the lower mounting plate and are used for accommodating the motor main body.
As a further preferable mode, detection ports are respectively arranged at corresponding positions on the upper mounting plate and the lower mounting plate, the bottom of the detection port of the lower mounting plate is connected with a glass sheet in a sealing manner, and structural members with cross section shapes matched with the detection ports are arranged on the glass sheet; when the upper mounting plate and the lower mounting plate are mutually fixed, the glass sheet, the structural member and the detection port of the upper mounting plate jointly define a water tank with an open top and a transparent bottom; the two ends of the bristle tubule are respectively rotatably arranged between the upper mounting plate detection port and the structural member through a first bearing, and each joint of the water tank is subjected to sealing treatment. When the water tank is filled with water, the bristle tubules are immersed in the water, so that the imaging quality can be improved.
In the present invention, all the soft capillaries (including the transition hose) are the same type of soft capillaries having the same inner diameter.
Compared with the prior art, the invention has the beneficial effects that:
according to the small-sized model animal automatic loading and fixing detection system, a siphon pipeline is constructed by using a soft bristle tubule according to a siphon principle, so that the animal is loaded and fixed without a pump, and the imaging analysis of the animal is completed by combining an imaging analysis unit; by controlling the siphon flow rate, the animal is quickly and automatically fixed under the condition of not carrying out gel embedding or long-time anesthesia, the time for fixing is effectively shortened, and the time for fixing the small model animal can be shortened by 100 times compared with the traditional manual method on average. The detection system is simple in structure, convenient to operate, economical and easy to popularize and apply; the method realizes the automatic loading and fixing detection of small animals in high-throughput, automatic and pump-free loading and non-microfluidic chip modes, and provides a great potential technical support for further realizing the research and the screening of complex disease treatment medicines and the related research of other small animals.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is an exploded view of the securing assembly and bristle tubule, stepper motor;
FIG. 3 is an image of different poses of an animal acquired using the detection system of the present invention; wherein A is a zebra fish larva dorsad graph; b is a zebra fish larva ventral graph; c is a lateral diagram of zebra fish larvae.
In the figure: 1-soft capillary tube, 11-transition hose, 2-fixed subassembly, 21-bristle tubule, 22-three way connection, 23-first bearing, 24-upper mounting plate, 241-second mounting groove, 242-motor dodge mouth, 243-image acquisition mouth, 244-first mounting groove, 25-second bearing, 26-stepper motor, 27-lower mounting plate, 28-glass plate, 29-baffle, 31-nutrient solution container, 32-animal container, 4-liquid level sensor, 5-peristaltic pump, 61-animal collector, 62-culture solution collector, 71-first pinch valve, 72-second pinch valve, 73-third pinch valve, 74-fourth pinch valve, 8-microscopic imaging system.
Detailed Description
In order to make the objects, technical schemes and technical effects of the present invention clearer, the present invention will be further described in detail with reference to specific embodiments and drawings thereof. It should be understood that the specific embodiments described in this specification are only for the purpose of illustrating the technical solution of the present invention and are not intended to limit the present invention.
As shown in fig. 1, a small-sized animal automatic loading and fixing detection system comprises a loading container, an imaging analysis unit, a receiving container and a peristaltic pump 5.
The loading container comprises a liquid container 31 and an animal container 32 which are arranged up and down, the top of the liquid container 31 is opened, and the bottom of the liquid container is connected with the top of the animal container 32 through a soft capillary 1; the lower portion of the animal container 32 is funnel-shaped.
The receiving container comprises a liquid receiver 62 and an animal collector 61, which are connected by a pipe. The liquid level in the liquid container 31 is higher than the liquid level in the liquid receiving container 62, and there is a difference in level between the two liquid levels to provide a suitable flow rate for siphoning.
The imaging analysis unit comprises a microscopic imaging system 8 and a computer (not shown) which are connected through signals, wherein a transparent bristle tubule 21 with the inner diameter matched with the width of an animal body is arranged on a stage of the microscopic imaging system 8 (comprising an inverted microscope and a microscopic camera).
The first end of the bristle tubule 21 is connected with the bottom opening of the animal container 32 and the animal collector 61 through a three-way joint 22 and two soft capillaries 1, respectively; the second end of the bristle tubule 21 is connected to the bottom of the liquid container 31 and the liquid receiver 62, respectively, through a transition hose 11 (soft capillary) and a three-way joint 22 and two soft capillaries 1, respectively. The inner diameter of the soft capillary tube 1 is slightly larger than the inner diameter of the bristle thin tube 21 and is slightly smaller than the outer diameter thereof.
A first pinch valve 71 is arranged on the soft capillary tube 1 of which the first end is connected with the bottom opening of the animal container 32, and a fourth pinch valve 74 is arranged on the soft capillary tube 1 connected with the animal collector 61; the transition hose 11 is provided with a second pinch valve 72 on the flexible capillary 1 connected to the bottom of the liquid container 31, and a third pinch valve 73 on the flexible capillary 1 connected to the liquid receiver 62. Each pinch valve is connected with a computer through signals, and the computer controls the opening and closing of the pinch valves.
A stopper (not shown) is fixedly installed in the bristle tubule 21, and serves to block the advance of the animal. The manufacturing and mounting process of the limiting piece is as follows: winding a metal wire on the side wall of the metal tube in a mode of carrying out multiple inner and outer interpenetration between two ends of the stainless steel metal tube to form a limiting piece composed of the metal tube and the metal wire; the main body part of the limiting member is arranged in a set position of the bristle tubule, one end of the metal wire is hooked on the second end of the bristle tubule 21, and the limiting member is fixed in the bristle tubule 21 through interference fit between the transition hose 11 and the bristle tubule 21.
The detection system also comprises a hollow shaft stepping motor 26, and the stepping motor 26 is arranged on the objective table through the fixing component 2; the second end of the bristle tubule 21 is coaxially fixed in the hollow shaft after being assembled with the transition hose 11; the stepper motor 26 is in signal communication with a computer which is capable of controlling operation of the stepper motor 26 to drive the bristle tubule 21 about an axis.
The fixing assembly 2 comprises an upper mounting plate 24 and a lower mounting plate 27, and the upper mounting plate 24 is connected with the lower mounting plate 27 through bolts and screws; the two ends of the hollow shaft of the stepping motor 26 are respectively rotatably arranged between the upper mounting plate 24 and the lower mounting plate 27 through the second bearing 25, and second mounting grooves 241 for mounting the second bearing 25 are respectively arranged at the corresponding positions of the upper mounting plate 24 and the lower mounting plate 27; motor avoiding openings 242 are distributed at corresponding positions on the upper mounting plate 24 and the lower mounting plate 27 and are used for accommodating the motor main body.
The corresponding positions of the upper mounting plate 24 and the lower mounting plate 27 are respectively provided with a detection port 243, the bottom of the detection port 243 of the lower mounting plate 27 is connected with a glass sheet 28 in a sealing way, and the glass sheet 28 is provided with a structural member 29 with a cross section shape matched with that of the detection port 243; when the upper mounting plate 24 and the lower mounting plate 27 are fixed with each other, the glass sheet 28, the structural member 29 and the detection port 243 of the upper mounting plate 24 together define a water tank with a transparent top opening and a transparent bottom; the two ends of the bristle tubule 21 are respectively rotatably mounted between the detection port 243 of the upper mounting plate 24 and the structural member 29 through the first bearings 23, the positions of the upper mounting plate 24 corresponding to the structural member 29 are respectively provided with a first mounting groove 244 for mounting the first bearings 23, and the joints of the water grooves are sealed.
The inlet and outlet of peristaltic pump 5 are respectively connected with liquid receiver 61 and liquid container 31 by means of flexible tubes, so that the nutrient solution can be recycled. The liquid container 31 is provided with a liquid level sensor 4, and the liquid level sensor 4 is used for monitoring the liquid level in the liquid container 31 and keeping the liquid level in the liquid container 31 at a set height according to the operation of the peristaltic pump 5.
The working principle of the detection system is as follows:
after the installation of the device is completed, the bristle tubules 21 are immersed into a water tank filled with water; in the initial state, all four pinch valves are in the closed state. First, the first pinch valve 71 and the third pinch valve 73 are opened, and the animals flow from the animal container 32 to the liquid receiver 62 one by one with the nutrient solution due to the existence of the liquid level difference, and the loading is started; when the first animal is loaded into the bristle tubule 21 and blocked by the limiting member, the microscopic imaging system captures the animal and sends a command through the computer, the first pinch valve 71 and the third pinch valve 73 are closed, the liquid stops flowing, the first animal is fixed in the detection area for imaging by the microscopic imaging system, and the loading is completed; the computer receives and analyzes the imaging result, controls the stepping motor 25 to drive the bristle tubule 21 to rotate according to the requirement, adjusts the animal posture to a required state, and then images the animal through the microscopic imaging system. After the imaging is completed, the second pinch valve 72 and the fourth pinch valve 74 are opened, and the nutrient solution flows from the liquid container 31 to the animal collector 61 under the driving of gravity, and in the process, the first animal flows to the animal collector 61 along with the nutrient solution, so that unloading is completed. After unloading is completed, the second pinch valve 72 and the fourth pinch valve 74 are closed, the first pinch valve 71 and the third pinch valve 73 are opened, and the next animal is loaded.
The liquid receiver 61 is connected with the animal collector 62 through a hose, and when the nutrient solution in the animal collector 62 automatically flows into the liquid receiver 61, the liquid level of the two liquid receivers is kept consistent. When the liquid level sensor 4 senses that the nutrient solution in the liquid container 31 is small, the liquid level sensor 4 sends a signal to the peristaltic pump 5, and the peristaltic pump 5 works to supplement the nutrient solution in the liquid receiver 62 into the liquid container 31, so that the nutrient solution in the liquid container 31 reaches a set level.
Application example:
the inner diameter of the soft capillary tube is 800 mu m, the inner diameter of the borosilicate thin-wall capillary tube is 750 mu m, and a round angle is designed; the limiting piece is obtained by axially winding a 0.13mm copper wire on a stainless steel metal tube with the outer diameter of 0.71mm and the inner diameter of 0.41mm and the total length of 19mm for two to three times; the liquid level difference between the liquid container and the liquid receiver is kept between 220 and 240mm.
The zebra fish larvae are used as small model animals for loading and fixing detection, and fig. 3 is a back (A in fig. 3), abdomen (B in fig. 3) and side (C in fig. 3) imaging image of the zebra fish larvae fixed in bristle tubules of the automatic loading and fixing detection system for the small model animals.
The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (9)
1. The automatic loading and fixing detection system for the small model animals is characterized by comprising a loading container, an imaging analysis unit, a receiving container and a detection unit for detecting the positions of the animals;
the level of the liquid level in the loading container is higher than the level of the liquid level in the receiving container;
the imaging area of the imaging analysis unit is provided with a bristle tubule, and two ends of the bristle tubule are respectively connected with the bottom of the loading container and the top of the receiving container through the soft capillary tubule;
at least one soft capillary tube is provided with a flow control element which is in signal connection with the detection unit;
when the detection unit detects that the animal enters the bristle tubule, the flow control element controls the fluid to stop flowing, so that the animal stays for imaging analysis by the imaging analysis unit.
2. The automated small form factor animal loading fixture detection system of claim 1, wherein the hard capillary has an inner diameter that matches the body width of the animal;
the inner diameter of the soft capillary tube is larger than the inner diameter of the hard capillary tube and smaller than the outer diameter of the bristle tubule.
3. The automatic loading and fixing detection system for small model animals according to claim 1, wherein the loading container comprises a liquid container and an animal container with a Murphy's dropper-like structure, which are arranged up and down;
the top of the liquid container is opened, and the bottom of the liquid container is connected with the top of the animal container through a soft capillary tube;
the lower part of the animal container is funnel-shaped, and the bottom opening is connected with the bristle tubule through the soft capillary tube.
4. The automatic loading and fixing detection system for small animals according to claim 3, wherein a limiting member for blocking the advance of the animals is arranged in the bristle tubule.
5. The automated small form factor animal loading fixture detection system of claim 4, wherein the first end of the bristle tubule is connected to the animal container bottom opening and the receiving container by a three-way connector and two flexible capillaries, respectively;
the second ends of the bristle tubules are respectively connected with the bottom of the liquid container and the receiving container through a three-way joint and two soft capillaries;
four soft capillaries connected with the bristle capillaries through three-way connectors are respectively provided with a flow control element, and each flow control element is respectively connected with the detection unit in a signal way.
6. The automated small form factor animal loading fixture detection system of claim 5, wherein the receiving container comprises a liquid receiver and an animal collector connected by a hose;
the bristle tubules are connected at a first end to an animal collector and at a second end to a liquid receiver.
7. The automated small form factor animal loading fixture detection system of claim 1, further comprising a hollow shaft stepper motor, the exit end of the bristle tubule when loaded being coaxially fixed within the hollow shaft of the stepper motor;
the imaging analysis unit is in signal connection with the stepping motor and controls the stepping motor to drive the bristle tubule to rotate around the shaft.
8. The automated small form factor animal loading fixture detection system of claim 1, wherein the imaging analysis unit comprises a signal-connected microscopic imaging system and computer;
the detection unit comprises a microscopic imaging system and a computer which are connected through signals.
9. The automated small form factor animal loading fixture detection system of claim 1, further comprising peristaltic pumps having inlets and outlets in communication with the liquid receiver and liquid container, respectively, for recycling of the nutrient solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310682671.XA CN116735590A (en) | 2023-06-09 | 2023-06-09 | Automatic loading and fixing detection system for small-sized model animals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310682671.XA CN116735590A (en) | 2023-06-09 | 2023-06-09 | Automatic loading and fixing detection system for small-sized model animals |
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| Publication Number | Publication Date |
|---|---|
| CN116735590A true CN116735590A (en) | 2023-09-12 |
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| CN202310682671.XA Pending CN116735590A (en) | 2023-06-09 | 2023-06-09 | Automatic loading and fixing detection system for small-sized model animals |
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| Country | Link |
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| CN (1) | CN116735590A (en) |
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