WO2015071832A1

WO2015071832A1 - A fluid transferring device

Info

Publication number: WO2015071832A1
Application number: PCT/IB2014/065979
Authority: WO
Inventors: Murali CHERAT; Swathi SUNDAR RAJ; Anish SEN MAJUMDAR; Abhijeet DESHMUKH; Manjunath BYALAPPA SATHYA KUMAR; Prajod THIRUVAMPATTIL LOHIDHAKSHAN
Original assignee: Stempeutics Research Private Limited
Priority date: 2013-11-13
Filing date: 2014-11-12
Publication date: 2015-05-21
Also published as: IN2013CH05149A

Abstract

A fluid transferring device comprising at least one dispensing container and at least one receiving container coupled to a first robotic assembly and a second robotic assembly respectively. First robotic assembly is configured to revolve the at least one dispensing container in a horizontal rotary axis towards each of the at least one receiving container. The second robotic assembly is configured to traverse the at least one receiving container towards the at least one dispensing container. Aligning and mating a dispensing port of the at least one dispensing container with an inlet port of the at least one receiving container. At least one valve mechanism installed at neck portion of the at least one dispensing container and the at least one receiving container for regulating flow of at least one of fluids such as biological tissue fluid, buffer solutions, enzymes and reagents.

Description

TITLE; A FLUID TRANSFERRING DEVICE

TECHNICAL FIELD

The present disclosure relates to a fluid transferring device. More particularly relates to the fluid transferring device equipped with a synchronized valve assembly or mechanism for transfer of fluid from one container to another container.

BACKGROUND OF DISCLOSURE Generally, controlling the flow of fluid (including gases, liquids, fluidized solids and slurries) are carried out by the use of valves or valve systems equipped in various fluid flow systems. Fluid flow systems are of various types, these include for e.g., delicate transfer of biological tissue fluid from containers in the laboratories to high pressure gushing of water in dams, rivers reservoirs etc. The valves play an important role in directing, controlling and regulating the flow of a fluid from one point to another. Valves are generally installed at crucial passageways in the fluid flow paths for regulating the flow of the fluid based on the requirement and time intervals. The valves can be of manual type operation or can be completely automated and programmed by the use of control systems. In cases of large scale applications, actuation valves such as electrically operated valves, hydraulically operated valves are utilized. However, for several small scale applications, manual valve operation such as hand operated valve mechanism, foot and knee operated valve mechanism will suffice. The valves arc of different types based on the requirement and the type of process system as explained above. Many of the common valves range from hydraulic, pneumatic, manual, solenoid and motor operated valves. Various other types of vales are gate valves, ball valves, needle valves, butterfly valves and check valves etc.,. These above mentioned valves can be used for obtaining and controlling the desired fluid flow rate.

There are different types of valves for various fluid flows and the most important and common flows are of pressurized fluid flow and gravitational flow due to the action of gravitational force.

A pressurized fluid flow uses pumps fixed at crucial locations within the flow system to pump the fluid from one point to another. This kind of pressurized flow is generally used to transfer fluids through certain distances by means of channels, tubes, hoses etc. This kind of transfer of fluid is used in cases where there are no delicate elements to be transferred, for e.g. transfer of water from a storage tank for domestic usage, fuel injection systems etc. Moreover, the valve systems equipped in such pressurized flow systems are very durable and can withstand high pressures and stresses. These valve systems can be programmed accordingly for opening and closing of the valve to obstruct and allow the fluid flow.

However, in other industrial processes such as biological/medical laboratories, tissue culturing and processing institutes, the flow of fluid has to be in a controlled and precise manner. Transfer of elements from one point to another has to be done carefully and delicately as the elements for transfer can be very delicate in nature and hence the flow of fluid can take place due to the effect of gravity only. Also, in conventional transfer of fluids, transfer from one point to another was slow since the fluids had to travel through tubes/hoses from a dispensing container to the receiving container. Moreover, use of pressure pumps to speed up the fluid flow process damages the delicate elements within the fluid rendering the particular process useless. Furthermore, the flow of fluid within the tube may lead to temperature differences which can cause drastic changes to the tissues within the fluid.

Synchronized opening of valve systems find its application in fluid flow systems where the opening of the flow lines have to be opened synchronously for allowing the flow of fluid in a controlled and timely manner. Valves are basically of ball valves, needle valves, disc valves etc. These types of valves are generally used in flow processes where the flow of the fluid occurs due to the gravitational forces.

Hence, there is a need to develop a valve assembly or mechanism which eradicates the use of tube or hoses as a means for transferring fluid between the dispensing container (also termed as storage container) and the receiving container. There also a further need to develop synchronized valve system which aids in transferring of fluid from the dispensing container to the receiving container directly wherein no pumps for developing pressurized flow of fluid is required. SUMMARY OF THE DISCLOSURE

One or more shortcomings of the prior art are to overcome and show additional advantages which are provided through the present disclosure. Additional features and advantages are realized through the techniques of the present disclosure. Other embodiments and aspects of the disclosure are described in detail herein and are considered a part of the claimed disclosure.

Accordingly, in one non-limiting embodiment of the present disclosure a fluid transferring device comprising: at least one dispensing container and at least one receiving container coupled to a first robotic assembly and a second robotic assembly respectively, the first robotic assembly is configured to revolve the at least one dispensing container in a horizontal rotary axis towards each of the at least one receiving container. The second robotic assembly is configured to traverse the at least one receiving container towards the at least one dispensing container. Aligning and mating a dispensing port of the at least one dispensing container with an inlet port of the at least one receiving container. At least one valve mechanism installed at neck portion of the at least one dispensing container and the at least one receiving container for regulating flow of at least one of biological tissue fluid, buffer solutions, enzymes and reagents. At least one sensor installed at mouth of the dispensing port of the at least one dispensing container and the inlet port of the at least one receiving container, wherein the at least one sensor senses mating of the dispensing container and receiving container to operate the valve mechanism.

In one embodiment of the present disclosure, the second robotic assembly equipped with the receiving container traverses in a multi plane axis towards the dispensing container for receiving the fluid and for performing different processing actions.

In one embodiment of the present disclosure, the fluid which is at least one of biological tissue fluid, buffer solutions, enzymes and reagents dispense from the dispensing container into the receiving container due to gravitational pull.

In one embodiment of the present disclosure, the first control unit which is interfaced with the first robotic assembly, rotates the at least one dispensing container in a horizontal rotary axis.

In one embodiment of the present disclosure, a second control unit is interfaced with the second robotic assembly to traverse the at least one receiving container towards the at least one dispensing container.

In one embodiment of the present disclosure, at least one sensor installed at mouth of the dispensing port of the at least one dispensing container and the inlet port of the at least one receiving container operate synchronously. In one embodiment of the present disclosure, at least one sensor provided on the at least one dispensing container and the at least one receiving container are interfaced to a drive motor for operating the at least one valve mechanism. In one embodiment of the present disclosure, a method of transferring tissue to a device comprising steps of: coupling at least one dispensing container and at least one receiving container to a first robotic assembly and a second robotic assembly, wherein; the first robotic assembly is configured to revolve the at least one dispensing container in a horizontal rotary axis towards each of the at least one receiving container. Coupling the second robotic assembly to the at least one receiving container, wherein the second robotic assembly is configured to: traverse the at least one receiving container towards each of the at least one dispensing container. Aligning and mating dispensing port of the at least one dispensing container, with an inlet port of each of the at least one receiving container. Installing at least one valve mechanism at neck portion of the at least one dispensing container and the at least one receiving container. Installing at least one sensor at mouth of the dispensing port of the at least one dispensing container and inlet port of each of the at least one receiving container senses mating of the dispensing container and receiving container to operate the valve mechanism for regulating flow of at least one of biological tissue fluid, buffer solutions, enzymes and reagents.

The foregoing summary is illustrative only and is not intended to be in any way limiting. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features will become apparent by reference to the drawings and the following detailed description.

BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS

The novel features and characteristic of the disclosure are set forth in the appended description. The disclosure itself, however, as well as a preferred mode of use, further objectives and advantages thereof, will best be understood by reference to the following detailed description of an illustrative embodiment when read in conjunction with the accompanying figures. One or more embodiments are now described, by way of example only, with reference to the accompanying figures wherein like reference numerals represent like elements and in which: Figure 1 illustrates fluid transfer device comprising a receiving container and a dispensing container each equipped with an intermediate valve mechanism driven by motor.

Figure 2 illustrates valve mechanisms of the fluid transfer device installed at each of the mouth of the containers with sensors.

Figure 3 illustrates perspecti ve view of the fluid transferring device.

The figures depict embodiments of the disclosure for purposes of illustration only. One skilled in the art will readily recognize from the following description that alternative embodiments of the structures and methods illustrated herein may be employed without departing from the principles of the disclosure described herein.

DETAILED DESCRIPTION OF THE DISCLOSURE

The foregoing has broadly outlined the features and technical advantages of the present disclosure in order that the detailed description of the disclosure that follows may be better understood. Additional features and advantages of the disclosure will be described hereinafter which form the subject of the claims of the disclosure. It should be appreciated by those skilled in the art that the conception and specific embodiment disclosed may be readily utilized as a basis for modifying or designing other structures for carrying out the same purposes of the present disclosure. It should also be realized by those skilled in the art that such equivalent constructions do not depart from the spirit and scope of the disclosure as set forth in the appended claims. The novel features which are believed to be characteristic of the disclosure, both as to its organization and method of operation, together with further objects and advantages will be better understood from the following description when considered in connection with the accompanying figures. It is to be expressly understood, however, that each of the figures is provided for the purpose of illustration and description only and is not intended as a definition of the limits of the present disclosure. It will be readily understood that the aspects of the present disclosure, as generally described herein, and illustrated in the figures, can be arranged, substituted, combined, and designed in a wide variety of different configurations, all of which are explicitly contemplated and make part of this disclosure.

Referring now to the drawings wherein the drawings are for the purpose of illustrating an exemplary embodiment of the disclosure only, and not for the puipose of limiting the same. The fluid transferring device is used to transfer biological tissue fluid, buffer solutions, enzymes and reagents without the use of any tubes or hoses. Also, the fluid transferring device transfers the above mentioned samples only due to the action of gravitational pull. At least one dispensing container is coupled to a first robotic assembly wherein, the first robotic assembly revolves in a horizontal rotary axis in a single plane. At least one receiving container is coupled to a second robotic assembly which is configured to traverse the receiving container towards the dispensing container. The neck portions of each of the dispensing container and receiving containers are equipped with valve mechanism for regulating the flow of the biological tissue fluid, buffer solutions, enzymes and reagents. The valve mechanism is automatically operated to open and close the respective valves as per the defined logic preset by the user. The sensors which are installed at each of the neck portions of the containers sense the mating of the receiving container with the dispensing container and appropriately open the valve. The first robotic assembly and the second robotic assembly are controlled by a first control unit and the second control unit respectively. The valve mechanism can be individually programmed to open or close simultaneously, synchronously or sequentially based on the requirement. Moreover, the first control unit and the second control unit control's the movement of the receiving containers to each of the dispensing container and also to operate the opening and closing of the valve mechanism. Figure 1 illustrates tissue processing unit (100) comprising a dispensing container (101) and a receiving container (102). The dispensing container (101) is coupled to a first robotic assembly (103) (refer figure 3) which is configured to revolve the dispensing container (101) in a horizontal rotary axis. The receiving container (102) is coupled to a second robotic assembly (104) which is configured to move the receiving container (102) in a multiple axis and also to traverse the receiving container (102) to the dispensing container (101). Figure 1 depicts the dispensing container (101) and the receiving container (102) in mated position. The neck portion (106) of the dispensing container (101) and the receiving container (102) is installed with a valve mechanism (105) to regulate the flow of fluid such as biological tissue fluid, buffer solutions, enzymes and reagents.

In an embodiment of the present disclosure, the dispensing container (101) is provided with a dispensing port (101a) which dispenses the fluid such as biological tissue fluid, buffer solutions, enzymes and reagents into the receiving container (102) through an inlet port (102a). In one embodiment of the present disclosure, the fluid is a biological fluid.

In an embodiment of the present disclosure, the flow fluid such as biological tissue fluid, buffer solutions, enzymes and reagents from the dispensing container (101) into the receiving container ( 102) takes place only when the mating of the dispensing port ( 101 a) of dispensing container (101) with that of the inlet port (102a) of the receiving container (102) via regulation or controlled sensing by the at least one sensor (107).

In an embodiment of the present disclosure, the mating of the dispensing container (101) and the receiving container (102) forms a passage for the flow of fluid such as biological tissue fluid, buffer solutions, enzymes and reagents eliminating the use of lubes or hoses.

In an embodiment of the present disclosure, the dispensing container (101) and the receiving container (102) are designed in such a way that, the neck portion (106) of the dispensing container (101) diverges to a single exit point [(i.e. dispensing port (101a)] for the flow of fluid such as biological tissue fluid, buffer solutions, enzymes and reagents.

In an embodiment of the present disclosure, the valve mechanism (105) installed at the neck portion (106) of the dispensing container (101) and the receiving container (102) is at least one of ball valve, butterfly valve, spool valve, check valve and any other valves which serve the purpose.

The sensors (107) are installed at mouth (108) (refer figure 2) of the dispensing port (101a) of the dispensing container (101) and also at the mouth (108) of the inlet port (102a) of the receiving container (102). The sensor (107) is programmed to sense the mating of the dispensing container (101) and the receiving container (102). When the inlet port (102a) of the receiving container (102) mates with the dispensing por (101a) of the dispensing container (101) the sensor triggers the valve mechanism (105) to operate [i.e. toggle between open and closed positions].

In an embodiment of the present disclosure, the valve mechanism (105) operates between open position and closed position simultaneously, synchronously or sequentially based on the requirement. In an embodiment of the present disclosure, the sensor (107) is at least one of proximity sensor, contact sensor, laser sensor and any other sensor which serves the purpose.

In an embodiment of the present disclosure, the receiving container (102) has an inlet port (102a) wherein, fluid such as the biological tissue fluid, buffer solutions, enzymes and reagents flows into the receiving container (102) from the dispensing container (101) eliminating the use of tubes or hoses.

In an embodiment of the present disclosure, the valve mechanism (105) is operated by a drive motor (111) which is at least one of gear drive, belt drive, chain drive or any other mechanism which serves the purpose. These drive motors (1 1 1) receives signals for operation from units such as a control unit, programmable logic controller or any other device which serves the purpose.

Figure 2 illustrates valve mechanism (105) of the fluid transfer device (100) installed at each of the mouth (108) of the containers (101 and 102) with sensors (107) and drive motor (111). The valve mechanism (105) consists of a ball valve mechanism which is mated to a drive motor (111). The drive motor (111) is in turn mated to a sensor (107) which senses the mating of the dispensing container (101) with the receiving container (102). Mating of the dispensing container (101) with the receiving container (102) eliminates the use of transfer means such as tubes or hoses. Thereby reducing the cost involved in manufacturing additional components for fluid transfer and also eliminates the need of performing maintenance operations such as cleaning of clogged tubes or hoses which is time consuming. Moreover, elimination of usage of tubes and hoses leads to sterile conditions during transfer of fluid from the dispensing container (101) into the receiving container (102). Also, since the transfer of fluid is taking place due to the gravitational forces or gravitational pull, the damage caused to the fluids is eliminated. Further, use of pressure pumps or external devices for fluid transfer is also eliminated. Moreover, diameter and path of tubes or hoses may limit fluid flow [for example; in case of fat to be transferred etc.]. Therefore, only specific size and shape of particles can flow through the lubes or hoses. Users have to choose specific diameters of tubes or hoses for specific size and shape of particles to be transferred which leads to operational downtime.

In an embodiment of the present disclosure, the neck portion (106) of each of the dispensing container (101) and the receiving container (102) consists of at least one valve mechanism (105). The neck portion (106) further consists of a dispensing port (101a) in the dispensing container (101) and an inlet port (102a) in the receiving container (102). Fluid such as the biological tissue fluid, buffer solutions, enzymes and reagents are transferred from the dispensing container (101) into the receiving container (102) through these dispensing and inlet ports (101a and 102a).

In an embodiment of the present disclosure, the sensor (107) is programmed to sense the mating of the dispensing container (101) and the receiving container (102). Once the sensor (107) senses this mating sequence, a signal is transmitted to the drive motor (111) which operates the valve mechanism (105) by rotating the valve mechanism (105) (i.e. ball valve in the present disclosure) in clockwise or anti-clockwise direction for allowing flow of fluid such as the biological tissue fluid, buffer solutions, enzymes and reagents from the dispensing container (101) into the receiving container (102). Also, the second control unit (110) controls the movement of the robotic ami (104) which traverses the receiving container (102) in position for alignment and mating with the dispensing container (101) for fluid transfer.

In an embodiment of the present disclosure, the drive motor (111) of the valve mechanism (105) is installed in line with each other so as to provide torque to the valve mechanism (105) for opening and closing during fluid transfer. In an embodiment of the present disclosure, the drive motor (111) and the sensors (107) are mounted on mounting brackets (112) which are fixed to the first robotic assembly (103) and the second robotic assembly (104) respectively.

In an alternative embodiment, the valve mechanism (105) can be programmed to operate opening and closing of the valve mechanism (105) simultaneously with some time lag between the opening of the valves. In other words, the valves are configured to work not only simultaneous but in sequential manner also.

Figure 3 illustrates perspective view of the fluid transferring device (100). The first robotic assembly (103) is equipped with plurality of dispensing containers (101) which are held in place by holders (not shown in figure). The first robotic assembly (103) is configured to revolve the plurality of dispensing containers (101) in a horizontal rotary axis or in any multiple axis movement. In an embodiment of the present disclosure, the first robotic assembly (103) can be configured to be stationary without imparting any rotational movement in a horizontal axis to the dispensing container (101). The second robotic assembly (104) consists of a receiving container (102) which is held in place firmly by a gripper (113). The second robotic assembly (104) has multiple degrees of freedom and can be moved to each of the dispensing containers (101) for transferring fluid such as biological tissue fluid, buffer solutions, enzymes and reagents from dispensing container (101) to the receiving container ( 102).

In an embodiment of the present disclosure, the first robotic assembly (103) and the second robotic assembly (104) is controlled by a first control unit (109) and the second control unit (110) respectively. The first control unit (109) and the second control unit (110) receives feedback from the sensors (107) and then operate the drive motor (111) for opening and closing the valve mechanism (105).

In an embodiment of the present disclosure, the second control unit (110) controls the movement of the second robotic assembly (104) wherein, the second robotic assembly (104) traverses to each of the dispensing container (101) for receiving the fluid such as biological tissue fluid, buffer solutions, enzymes and reagents. Alternatively, the first robotic assembly (101) maybe programmed to revolve the dispensing container (101) in a horizontal rotary axis so as to dispense fluid such as the biological tissue fluid, buffer solutions, enzymes and reagents into the receiving container (102).

ADVANTAGES

In one embodiment, usage of tubes and hoses as means for transferring fluid are eliminated, resulting in sterile fluid transfer.

In one embodiment, direct mating of containers negates usage of tubes or hoses and in turn avoids limitation relating to volume of fluid transfer. Also, operational downtime in choosing different lubes or hoses during tissue transfer is avoided.

In one embodiment, transfer of fluid takes place only due to the gravitational force or gravitational pull and no external means such as pressure pumps are used, which avoids damage to the contents during transferring operation.

In one embodiment, transfer of fluid is automated based on the programming and hence avoiding manual intervention. In one embodiment, the dispensing container is manufactured so as to have a single diverging dispensing port which aids in complete evacuation of the fluid from the dispensing container and avoids hung back condition of fluids.

In one embodiment, the rate of fluid flow can be automatically controlled by the user by controlling the opening and closing of the valve mechanism and thereby avoiding inadequate or surplus fluid flow.

EQUIVALENTS

With respect to the use of substantially any plural and or singular terms herein, those having skill in the art can translate from the plural to the singular and/or from the singular to the plural as is appropriate to the context and/or application. The various singular/plural permutations may be expressly set forth herein for sake of clarity.

It will be understood by those within the art that, in general, terms used herein, and especially in the appended claims (e.g., bodies of the appended claims) are generally intended as "open" terms (e.g., the term "including" should be interpreted as "including but not limited to," the term "having" should be interpreted as "having at least," the term "includes" should be interpreted as "includes but is not limited to," etc.). It will be further understood by those within the art that if a specific number of an introduced claim recitation is intended, such an intent will be explicitly recited in the claim, and in the absence of such recitation no such intent is present. For example, as an aid to understanding, the following appended claims may contain usage of the introductory phrases "at least one" and "one or more" to introduce claim recitations. However, the use of such phrases should not be construed to imply that the introduction of a claim recitation by the indefinite articles "a" or "an" limits any particular claim containing such introduced claim recitation to inventions containing only one such recitation, even when the same claim includes the introductory phrases "one or more" or "at least one" and indefinite articles such as "a" or "an" (e.g., "a" and/or "an" should typically be interpreted to mean "at least one" or "one or more"); the same holds true for the use of definite articles used to introduce claim recitations. In addition, even if a specific number of an introduced claim recitation is explicitly recited, those skilled in the art will recognize that such recitation should typically be interpreted to mean at least the recited number (e.g., the bare recitation of "two recitations," without other modifiers, typically means at least two recitations, or two or more recitations). Furthermore, in those instances where a convention analogous to "at least one of A, B, and C, etc." is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., "a system having at least one of A, B, and C" would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). In those instances where a convention analogous to "at least one of A, B, or C, etc." is used, in general such a construction is intended in the sense one having skill in the art would understand the convention (e.g., "a system having at least one of A, B, or C" would include but not be limited to systems that have A alone, B alone, C alone, A and B together, A and C together, B and C together, and/or A, B, and C together, etc.). It will be further understood by those within the art that virtually any disjunctive word and/or phrase presenting two or more alternative terms, whether in the description, claims, or drawings, should be understood to contemplate the possibilities of including one of the terms, either of the terms, or both terms. For example, the phrase "A or B" will be understood to include the possibilities of "A" or "B" or "A and B."

While various aspects and embodiments have been disclosed herein, other aspects and embodiments will be apparent to those skilled in the art. The various aspects and embodiments disclosed herein arc for purposes of illustration and arc not intended to be limiting, with the true scope and spirit being indicated by the following claims.

REFERENCE NUMERALS

111 Drive motor

112 Mounting bracket

113 Gripper

Claims

1. A fluid transferring device (100) comprising:

at least one dispensing container (101) and at least one receiving container (102) coupled to a first robotic assembly (103) and a second robotic assembly (104) respectively,

the first robotic assembly (103) is configured to revolve the at least one dispensing container (101) in a horizontal rotary axis towards each of the at least one receiving container (102);

the second robotic assembly (104) is configured to:

traverse the at least one receiving container (102) towards the at least one dispensing container (101);

aligning and mating a dispensing port (101a) of the at least one dispensing container (101) with an inlet port (102a) of the at least one receiving container (102);

at least one valve mechanism (105) installed at neck portion (106) of the at least one dispensing container (101) and the at least one receiving container (102) for regulating flow of fluid which is at least one of biological tissue fluid, buffer solutions, enzymes and reagents;

at least one sensor (107) installed at mouth ( 108) of the dispensing port (101a) of the at least one dispensing container (101) and the inlet port (102a) of the at least one receiving container (102), wherein the at least one sensor (107) senses mating of the dispensing container (101) and receiving container (102) to operate the valve mechanism (105).

2. The device (100) as claimed in claim 1 , wherein the second robotic assembly (104) equipped with the receiving container (102) traverses in a multi plane axis towards the dispensing container (101) for receiving the fluid and for performing different processing actions.

3. The device (100) as claimed in claim 1, wherein the fluid such as biological tissue fluid, buffer solutions, enzymes and reagents dispense from the dispensing container (101) into the receiving container (102) due to gravitational force or gravitational pull.

4. The device (100) as claimed in claim 1 comprises first control unit (109) that is interfaced with the first robotic assembly (103) to rotate the at least one dispensing container (101) in a horizontal rotary axis.

5. The device (100) as claimed in claim 1 comprises a second control unit (110) that is interfaced with the second robotic assembly (110) to traverse the at least one receiving container (102) towards the at least one dispensing container (101).

6. The device (100) as claimed in claim 1, wherein the at least one sensor (107) installed at mouth (108) of the dispensing port (101a) of the at least one dispensing container

(101) and the inlet port (102a) of the at least one receiving container (102) operate synchronously.

7. The device (100) as claimed in claim 1, wherein the at least one sensor (107) provided on the at least one dispensing container (101) and the at least one receiving container

(102) are interfaced to a drive motor (111) for operating the at least one valve mechanism (105).

8. A method of transferring tissue to a device (100) comprising steps of:

coupling at least one dispensing container (101) and at least one receiving container (102) to a first robotic assembly (103) and a second robotic assembly (104), wherein;

the first robotic assembly (103) is configured to:

revolve the at least one dispensing container (101) in a horizontal rotary axis towards each of the at least one receiving container (102);

coupling the second robotic assembly (104) to the at least one receiving container (102), wherein the second robotic assembly (104) is configured to:

traverse the at least one receiving container (102) towards each of the at least one dispensing container (101);

aligning and mating a dispensing port (101a) of the at least one dispensing container (101) with an inlet port (102a) of each of the at least one receiving container (102); installing at least one valve mechanism (105) at neck portion (106) of the at least one dispensing container (101) and the at least one receiving container (102) and

installing at least one sensor (107) at mouth (108) of the dispensing port (101 a) of the at least one dispensing container (101) and inlet port (102a) of each of the at least one receiving container (102) senses mating of the dispensing container (101) and receiving container (102) to operate the valve mechanism (105) for regulating flow of fluid such as biological tissue fluid, buffer solutions, enzymes and reagents.

9. A fluid transferring device (100) as claimed in claim 1 , used for transferring fluid.