CN116730486B - 一种厌氧氨氧化菌增生剂 - Google Patents
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
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- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
本发明公开了一种厌氧氨氧化菌增生剂，属于污水处理技术领域。收集高负荷厌氧氨氧化反应器出水作为增生液；向增生液中补充叶酸钠、葡萄糖酸亚铁制备得到增生剂。所述的高负荷厌氧氨氧化反应器是指运行温度在30~37℃，废水总氮浓度在0.5~1.5 g/L、污泥浓度在5~15 gVSS/L，容积总氮去除负荷在2~15 gN/L/d，出水溶解性总有机碳浓度在20~50 mgC/L的厌氧氨氧化反应器。经试验证明，本方法制备出的增生剂可降低厌氧氨氧化菌的细胞合成成本，提高厌氧氨氧化菌的生长速率，缩短新反应器的启动时间。本发明的制备步骤简洁、增生效率高，适用于多种工况下厌氧氨氧化反应器的快速启动，具有巨大的经济价值和环保价值。
Description
技术领域
本发明涉及一种厌氧氨氧化菌增生剂。
背景技术
厌氧氨氧化工艺因其无需外加有机碳源、脱氮负荷高、运行费用低、占地空间小等优点成为目前最经济的生物脱氮工艺之一。厌氧氨氧化脱氮技术已成功应用于处理污泥消化液、垃圾渗滤液、禽畜养殖废水等高氨废水处理。
然而，厌氧氨氧化菌在低温低基质条件下的原位富集并表现出能适用于工程化的活性，是一个非常漫长且困难的过程。其原因主要由于厌氧氨氧化菌的两个生理特性：1）厌氧氨氧化菌存在代谢缺陷，无法合成自身生长所需叶酸和钼辅因子等物质，需要依赖伴生菌提供诸多生物素。如果不考虑细胞衰亡和污泥流失，在20oC时，厌氧氨氧化菌的富集时间只要需要35~55天以上。2）厌氧氨氧化菌的活性具有鲜明的密度依懒性。当厌氧氨氧化菌密度低于5 × 106copies/L时，无法检测出活性。外源信号分子可以强化厌氧氨氧化菌在低温低基质条件下的活性。但是，在实际工程中，长期添加造价昂贵的外源信号分子并不具有成本效益。
因此，如何从源头降低厌氧氨氧化菌的细胞合成成本，经济高效地提高厌氧氨氧化菌的生长速率是缩短新反应器的启动时间的关键难题。
发明内容
为了克服现有技术的不足，本发明的目的是提供公开了一种厌氧氨氧化菌增生剂。
一种厌氧氨氧化菌增生剂，收集高负荷厌氧氨氧化反应器出水作为增生液；
向增生液中补充叶酸钠、葡萄糖酸亚铁制备得到增生剂。
所述的高负荷厌氧氨氧化反应器是指运行温度在30~37℃，废水总氮浓度在0.5~1.5 g/L、污泥浓度在5~15 gVSS/L，容积总氮去除负荷在2~15 gN/L/d，出水溶解性总有机碳浓度在20~50 mgC/L的厌氧氨氧化反应器。
所述的叶酸钠的投加量为10~30 mg/L，葡萄糖酸亚铁为20~50 mg/L，调节增生剂的pH为8.0~9.0。
所述的一种厌氧氨氧化菌增生剂，将增生剂和进水按1:25~50体积比例投加到新反应器中。
所述的新反应器采用活性污泥、反硝化污泥、厌氧颗粒污泥、沉积物为接种物时，增生剂采用高投加比，增生剂和进水按1:25~35体积比例，且投加时长为61~120天；新反应器采用保藏的厌氧氨氧化污泥为接种物时，增生剂采用低投加比，增生剂和进水按1:36~50体积比例，且投加时长为30~60天。
本发明的优点主要体现在：
制备步骤简洁，厌氧氨氧化菌增生效率高，适用于多种工况下厌氧氨氧化反应器的快速启动，具有巨大的经济价值和环保价值。
附图说明
图1是对照反应器(a)和试验反应器(b)启动过程中的脱氮性能。
具体实施方式
下面结合具体实施例和附图对本发明进行进一步描述，但本发明的保护范围并不仅限于此。
根据本发明所述的一种厌氧氨氧化菌增生剂，增生液取自在35 ± 1oC环境下运行6个月的厌氧氨氧化颗粒污泥反应器（工作体积1.5 L），该反应器进水总氮浓度为560 ±20 mg/L,污泥浓度约为15 gVSS/L，容积总氮去除负荷为6.2~6.4gN/L/d，出水溶解性总有机碳浓度为20~40 mgC/L。收集反应器出水并投加15 mg/L叶酸钠和25 mg/L葡萄糖酸亚铁制备增生剂（pH为8.7）。两台新启动的UASB反应器工作体积均为1.5 L，在22 ± 2oC环境下运行，采用4℃保藏两年的厌氧氨氧化污泥为接种物，以模拟城市污水为进水，总氮浓度为60 mg/L（氨氮：亚硝氮=1:1.2），进水量为15 L/d。对照反应器进水不加增生液，而试验反应器进水以1:40的比例投加增生剂。如图1所示，对照反应器在40天时厌氧氨氧化总氮去除率达到80%以上，而试验反应器在21天时总氮去除率已达到相同水平，启动时间缩短近一半。
可以理解的是，以上关于本发明的具体描述，仅用于说明本发明而并非受限于本发明实施例所描述的技术方案。本领域的普通技术人员应当理解，仍然可以对本发明进行修改或等同替换，以达到相同的技术效果；只要满足使用需要，都在本发明的保护范围之内。本发明的保护范围由所附权利要求及其任何等同技术方案给出。
Claims (1)
1.一种厌氧氨氧化菌增生剂，其特征在于，收集高负荷厌氧氨氧化反应器出水作为增生液；
向增生液中补充叶酸钠、葡萄糖酸亚铁制备得到增生剂；
所述的高负荷厌氧氨氧化反应器是指运行温度在30~37℃，废水总氮浓度在0.5~1.5g/L、污泥浓度在5~15 gVSS/L，容积总氮去除负荷在2~15 gN/L/d，出水溶解性总有机碳浓度在20~50 mgC/L的厌氧氨氧化反应器；
所述的叶酸钠的投加量为10~30 mg/L，葡萄糖酸亚铁为20~50 mg/L，调节增生剂的pH为8.0~9.0；
将增生剂和进水按1:25~50体积比例投加到新反应器中。
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101886056A (zh) * | 2010-07-02 | 2010-11-17 | 北京桑德环保集团有限公司 | 一种厌氧氨氧化菌的分离方法 |
| CN105254124A (zh) * | 2015-10-16 | 2016-01-20 | 巢湖市聚源机械有限公司 | 一种轧钢废水处理方法 |
| CN105417840A (zh) * | 2015-11-02 | 2016-03-23 | 安徽洋森环保节能科技有限公司 | 一种涂装前处理废水的处理方法 |
| CN112264015A (zh) * | 2020-11-23 | 2021-01-26 | 湖南速博生物技术有限公司 | 一种废水氧化处理催化剂的制备方法 |
| RU2751356C1 (ru) * | 2020-07-13 | 2021-07-13 | Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН) | Способ удаления азотсодержащих соединений из сточных вод |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101886056A (zh) * | 2010-07-02 | 2010-11-17 | 北京桑德环保集团有限公司 | 一种厌氧氨氧化菌的分离方法 |
| CN105254124A (zh) * | 2015-10-16 | 2016-01-20 | 巢湖市聚源机械有限公司 | 一种轧钢废水处理方法 |
| CN105417840A (zh) * | 2015-11-02 | 2016-03-23 | 安徽洋森环保节能科技有限公司 | 一种涂装前处理废水的处理方法 |
| RU2751356C1 (ru) * | 2020-07-13 | 2021-07-13 | Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН) | Способ удаления азотсодержащих соединений из сточных вод |
| CN112264015A (zh) * | 2020-11-23 | 2021-01-26 | 湖南速博生物技术有限公司 | 一种废水氧化处理催化剂的制备方法 |
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Denomination of invention: A growth agent for anaerobic ammonia oxidizing bacteria Granted publication date: 20240105 Pledgee: Guotou Taikang Trust Co.,Ltd. Pledgor: Zhejiang Wole Technology Co.,Ltd. Registration number: Y2024980011366 |
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