CN116687000A - Oyster peptide and saussurea involucrata active peptide composition and preparation method thereof - Google Patents
Oyster peptide and saussurea involucrata active peptide composition and preparation method thereof Download PDFInfo
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 134
- 241000237502 Ostreidae Species 0.000 title claims abstract description 128
- 235000020636 oyster Nutrition 0.000 title claims abstract description 128
- 241001145025 Saussurea involucrata Species 0.000 title claims abstract description 70
- 239000000203 mixture Substances 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 92
- 238000001914 filtration Methods 0.000 claims abstract description 33
- 238000001035 drying Methods 0.000 claims abstract description 29
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 239000000706 filtrate Substances 0.000 claims description 21
- 235000013372 meat Nutrition 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
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- 102000035195 Peptidases Human genes 0.000 claims description 7
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- 239000012535 impurity Substances 0.000 claims description 7
- 235000019833 protease Nutrition 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
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- 238000005406 washing Methods 0.000 claims description 7
- 108090000526 Papain Proteins 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 102000004142 Trypsin Human genes 0.000 claims description 6
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- 230000000975 bioactive effect Effects 0.000 claims description 6
- 229940055729 papain Drugs 0.000 claims description 6
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- 238000010298 pulverizing process Methods 0.000 claims description 6
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- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
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- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 6
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- 238000000108 ultra-filtration Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
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- 230000002292 Radical scavenging effect Effects 0.000 description 1
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- 239000003929 acidic solution Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
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- 238000010586 diagram Methods 0.000 description 1
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- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/008—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application provides a preparation method of an oyster peptide and saussurea involucrata active peptide composition, which comprises the steps of fully mixing pretreated oyster peptide powder and saussurea involucrata active peptide powder according to a proportion, filtering, concentrating, drying and the like after mixing, so as to obtain the oyster peptide powder and saussurea involucrata active peptide powder composition. The oyster peptide powder and saussurea involucrata active peptide powder composition prepared by the application has the advantages of rich nutrition, remarkable health care effect and the like, and can be widely applied to the fields of health care food, medicine and the like.
Description
Technical Field
The application belongs to the technical field of medicines and foods, and particularly relates to an oyster peptide and saussurea involucrata active peptide composition and a preparation method thereof.
Background
Oyster peptide is a nutritional health product, and the main component is peptide substances in oyster extract. Oyster peptide has various health care effects, such as improving immunity, promoting bone growth, improving sleep quality, regulating blood lipid, lowering blood pressure, etc. In addition, oyster peptide has the functions of resisting oxidation, fatigue, aging and the like, and is widely applied to the fields of health care products, cosmetics and the like. At present, the oyster peptide is mainly extracted by the following methods: acid hydrolysis: mixing oyster powder with acidic solution, reacting for a certain time, centrifuging, and filtering to obtain oyster peptide. And (3) enzymolysis: mixing oyster powder with specific enzyme, reacting for a certain time, centrifuging, and filtering to obtain oyster peptide. Ultrasonic method: mixing oyster powder with water, ultrasonic treating, centrifuging, and filtering to obtain oyster peptide. Ultrafiltration method: mixing oyster powder with water, and treating with ultrafiltration membrane to obtain oyster peptide. At present, oyster peptide extraction technology is mature, but different extraction methods have differences in the yield, purity, activity and the like of oyster peptide. Therefore, in practical applications, an appropriate extraction method needs to be selected according to the specific situation.
The saussurea involucrata active peptide is an active peptide extracted from saussurea involucrata, and has various biological activities, such as antioxidation, antibiosis, anti-inflammatory, anti-tumor and the like. It can promote immunity, regulate blood sugar and blood lipid, improve liver function, and inhibit growth and diffusion of tumor cells. Therefore, the saussurea involucrata active peptide is widely applied to the fields of health care products, medicines, cosmetics and the like.
In view of this, the present application has been proposed.
Disclosure of Invention
Aiming at the technical problems, the application provides a composition of oyster peptide and saussurea involucrata active peptide for the first time, and the composition combines the activities of the two components, and has very practical value in the fields of food and medicine.
Accordingly, a first object of the present application is to find a novel food or pharmaceutical composition comprising oyster peptide and saussurea involucrata active peptide.
A second object of the present application is to find a method for preparing the above food or pharmaceutical composition comprising oyster peptide and saussurea involucrata active peptide.
Specifically, the application firstly provides a preparation method of an oyster peptide and saussurea involucrata active peptide composition, which is characterized by comprising the following steps:
fully mixing oyster peptide powder and saussurea involucrata powder according to a certain proportion, filtering, concentrating and drying the mixture to obtain the oyster peptide powder and saussurea involucrata bioactive peptide powder composition.
Further, the ratio is 1:1-3.
Further, the preparation method of the oyster peptide powder comprises the following steps:
1) Selecting fresh oyster, removing shell and viscera, and cutting meat into pieces;
2) Placing oyster meat into a pot, and cooking with small fire;
3) Stirring the cooked oyster meat into paste;
4) Pouring oyster mud into a filter screen, and washing with clear water to remove impurities;
5) Drying the filtered oyster mud.
6) Adding oyster peptidase into the dried oyster mud, performing enzymolysis to obtain oyster peptide, and drying again to obtain oyster peptide powder.
Further, the preparation method of the saussurea involucrata active peptide powder comprises the following steps:
1) Pulverizing dried herba Saussureae Involueratae into powder, adding appropriate amount of distilled water, soaking, and filtering;
2) Adding a proper amount of trypsin and papain into the collected filtrate, and hydrolyzing at constant temperature;
3) Filtering the hydrolysate to remove residues and insoluble substances, and obtaining the active peptide filtrate containing the saussurea involucrata.
4) Concentrating and drying the filtrate to obtain the saussurea involucrata active peptide powder.
Further, the filtering is carried out by adopting a filter screen with 50-150 meshes;
further preferably, the filtration is performed using a 100-150 mesh screen.
Further, the concentration is normal pressure concentration or reduced pressure concentration.
Further, the drying is spray drying or vacuum freeze drying.
The application also provides an oyster peptide and saussurea involucrata active peptide composition, which comprises oyster peptide powder and saussurea involucrata active peptide powder.
The application also provides an oyster peptide and saussurea involucrata active peptide composition, which is prepared by the method.
The application has at least the following advantages:
the application provides a novel food composition for the first time, the composition contains oyster peptide and saussurea involucrata active peptide, the composition has higher antioxidant activity and higher nutrition and medicinal value, and can be widely applied to the fields of health-care food, medicine and the like. In addition, the preparation method of the composition is simple and the cost is low.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present application, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1, a process flow diagram of the present application.
Detailed Description
The following description of the embodiments of the present application will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the application are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The following terms or definitions are provided solely to aid in the understanding of the application. These definitions should not be construed to have a scope less than understood by those skilled in the art.
Unless defined otherwise hereinafter, all technical and scientific terms used in the detailed description of the application are intended to be identical to what is commonly understood by one of ordinary skill in the art. While the following terms are believed to be well understood by those skilled in the art, the following definitions are set forth to better explain the present application.
As used herein, the terms "comprising," "including," "having," "containing," or "involving" are inclusive or open-ended and do not exclude additional unrecited elements or method steps. The term "consisting of …" is considered to be a preferred embodiment of the term "comprising". If a certain group is defined below to contain at least a certain number of embodiments, this should also be understood to disclose a group that preferably consists of only these embodiments.
The indefinite or definite article "a" or "an" when used in reference to a singular noun includes a plural of that noun.
The terms "about" and "substantially" in this application mean the range of accuracy that one skilled in the art can understand yet still guarantee the technical effect of the features in question. The term generally means + -10% of the indicated value, preferably
±5％。
Furthermore, the terms first, second, third, (a), (b), (c), and the like in the description and in the claims, are used for distinguishing between similar elements and not necessarily for describing a sequential or chronological order. It is to be understood that the terms so used are interchangeable under appropriate circumstances and that the embodiments of the application described herein are capable of operation in other sequences than described or illustrated herein.
The preparation method of the oyster peptide and saussurea involucrata active peptide composition of the application mainly comprises the following steps:
fully mixing oyster peptide powder and saussurea involucrata powder according to a certain proportion, filtering, concentrating and drying the mixture to obtain the oyster peptide powder and saussurea involucrata bioactive peptide powder composition.
In some embodiments, the ratio is 1:1-3.
In some embodiments, the method of preparing oyster peptide powder comprises the steps of:
1) Selecting fresh oyster, removing shell and viscera, and cutting meat into pieces;
2) Placing oyster meat into a pot, and cooking with small fire;
3) Stirring the cooked oyster meat into paste;
4) Pouring oyster mud into a filter screen, and washing with clear water to remove impurities;
5) Drying the filtered oyster mud.
6) Adding oyster peptidase into the dried oyster mud, performing enzymolysis to obtain oyster peptide, and drying again to obtain oyster peptide powder.
In some embodiments, the preparation method of the saussurea involucrata active peptide powder is as follows:
1) Pulverizing dried herba Saussureae Involueratae into powder, adding appropriate amount of distilled water, soaking, and filtering;
2) Adding a proper amount of trypsin and papain into the collected filtrate, and hydrolyzing at constant temperature;
3) Filtering the hydrolysate to remove residues and insoluble substances, and obtaining the active peptide filtrate containing the saussurea involucrata.
4) Concentrating and drying the filtrate to obtain the saussurea involucrata active peptide powder.
In some embodiments, in the step of filtering, concentrating and drying the above mixture to obtain the oyster peptide powder and the saussurea involucrata active peptide powder composition, the filtering may be performed by using a 50-150 mesh filter screen; preferably, filtering with 100-150 mesh sieve; the concentration can be normal pressure concentration or reduced pressure concentration; the drying may be spray drying, vacuum freeze drying, or the like.
The following are specific examples.
EXAMPLE 1 preparation of oyster peptide powder and yacon active peptide powder composition example 1
Firstly, selecting fresh oyster, removing shells and viscera, and cutting meat into blocks; placing oyster meat into a pot, and cooking with small fire for 20 minutes; stirring the cooked oyster meat into paste; pouring oyster mud into a filter screen with the mesh of about 50 meshes, and washing with clear water to remove impurities; drying the filtered oyster mud; adding oyster peptidase into the dried oyster mud, performing enzymolysis to obtain oyster peptide, and drying again to obtain oyster peptide powder. Then, taking dried saussurea involucrata, pulverizing into powder, adding a proper amount of distilled water, soaking, and filtering and collecting; adding a proper amount of trypsin into the collected filtrate, and hydrolyzing at constant temperature; filtering the hydrolysate to remove residues and insoluble substances, and obtaining the active peptide filtrate containing the saussurea involucrata. Concentrating and drying the filtrate to obtain the saussurea involucrata active peptide powder. Fully mixing the prepared oyster peptide powder and the saussurea involucrata powder according to the proportion of 1:1, filtering the mixture by a 100 filter screen, concentrating under reduced pressure and spray drying to obtain the oyster peptide powder and saussurea involucrata bioactive peptide powder composition 1.
EXAMPLE 2 preparation of oyster peptide powder and yacon active peptide powder composition example 2
Firstly, selecting fresh oyster, removing shells and viscera, and cutting meat into blocks; placing oyster meat into a pot, and stewing with small fire for 30 minutes; stirring the cooked oyster meat into paste; pouring oyster mud into a filter screen with about 30 meshes, and washing with clear water to remove impurities; drying the filtered oyster mud; adding oyster peptidase into the dried oyster mud, performing enzymolysis to obtain oyster peptide, and drying again to obtain oyster peptide powder. Then, taking dried saussurea involucrata, pulverizing into powder, adding a proper amount of distilled water, soaking, and filtering and collecting; adding a proper amount of papain into the collected filtrate, and hydrolyzing at constant temperature; filtering the hydrolysate to remove residues and insoluble substances, and obtaining the active peptide filtrate containing the saussurea involucrata. Concentrating and drying the filtrate to obtain the saussurea involucrata active peptide powder. Fully mixing the prepared oyster peptide powder and the saussurea involucrata powder according to the proportion of 1:2, filtering the mixture by a 150 filter screen, concentrating under reduced pressure and spray drying to obtain the oyster peptide powder and saussurea involucrata bioactive peptide powder composition 2.
EXAMPLE 3 preparation of oyster peptide powder and yacon active peptide powder composition example 3
Firstly, selecting fresh oyster, removing shells and viscera, and cutting meat into blocks; placing oyster meat into a pot, and stewing with small fire for 30 minutes; stirring the cooked oyster meat into paste; pouring oyster mud into a filter screen with about 80 meshes, and washing with clear water to remove impurities; drying the filtered oyster mud; adding oyster peptidase into the dried oyster mud, performing enzymolysis to obtain oyster peptide, and drying again to obtain oyster peptide powder. Then, taking dried saussurea involucrata, pulverizing into powder, adding a proper amount of distilled water, soaking, and filtering and collecting; adding a proper amount of trypsin and papain into the collected filtrate, and hydrolyzing at constant temperature; filtering the hydrolysate to remove residues and insoluble substances to obtain a filtrate containing the saussurea involucrata active peptide; concentrating and drying the filtrate to obtain the saussurea involucrata active peptide powder. Fully mixing the prepared oyster peptide powder and the saussurea involucrata powder according to the proportion of 1:3, filtering the mixture by a 120 filter screen, concentrating under reduced pressure and spray drying to obtain the oyster peptide powder and saussurea involucrata bioactive peptide powder composition 3.
Example 4 oyster peptide powder preparation example 4
Firstly, selecting fresh oyster, removing shells and viscera, and cutting meat into blocks; placing oyster meat into a pot, and stewing with small fire for 30 minutes; stirring the cooked oyster meat into paste; pouring oyster mud into a filter screen with about 100 meshes, and washing with clear water to remove impurities; drying the filtered oyster mud; adding oyster peptidase into the dried oyster mud, performing enzymolysis to obtain oyster peptide, and drying again to obtain oyster peptide powder; filtering with 120 filter screen, concentrating under reduced pressure, and spray drying to obtain oyster peptide powder and saussurea involucrata active peptide powder composition 4.
Example 5 preparation of saussurea involucrata active peptide powder composition example 5
Firstly, taking a dried saussurea involucrata product, crushing the dried saussurea involucrata product into powder, adding a proper amount of distilled water, soaking the powder, and filtering and collecting the powder; adding a proper amount of trypsin and papain into the collected filtrate, and hydrolyzing at constant temperature; filtering the hydrolysate to remove residues and insoluble substances to obtain a saussurea involucrata active peptide-containing filtrate, concentrating and drying the filtrate to obtain saussurea involucrata active peptide powder, filtering the saussurea involucrata active peptide powder by a 120 filter screen, concentrating under reduced pressure, and spray-drying to obtain the oyster peptide powder and saussurea involucrata active peptide powder composition 5.
Example 6 comparison of antioxidant Activity of peptide powders prepared by different methods
The antioxidant activity was evaluated based on DPPH radical scavenging method using the same mass of the different peptide powder compositions prepared in examples 1 to 5 and the oyster peptide powder and the saussurea involucrata active peptide powder alone, and the specific comparison results are shown in the following table:
| preparation example 1 | Preparation example 2 | Preparation example 3 | Preparation example 4 | Preparation example 5 | |
| Free radical scavenging rate | 80％ | 85％ | 82％ | 65％ | 60％ |
Therefore, compared with independently prepared peptide powder, the oyster peptide powder and saussurea involucrata active peptide powder compound prepared by the application has higher antioxidant activity.
The foregoing descriptions of specific exemplary embodiments of the present application are presented for purposes of illustration and description. It is not intended to limit the application to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the application and its practical application to thereby enable one skilled in the art to make and utilize the application in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the application be defined by the claims and their equivalents.
Claims (9)
1. The preparation method of the oyster peptide and saussurea involucrata active peptide composition is characterized by comprising the following steps:
fully mixing oyster peptide powder and saussurea involucrata powder according to a certain proportion, filtering, concentrating and drying the mixture to obtain the oyster peptide powder and saussurea involucrata bioactive peptide powder composition.
2. The method for preparing oyster peptide powder and saussurea involucrata composition according to claim 1, wherein the ratio is 1:1-3.
3. The method for preparing the oyster peptide and saussurea involucrata composition according to claim 1 or 2, wherein the method for preparing the oyster peptide powder comprises the following steps:
1) Selecting fresh oyster, removing shell and viscera, and cutting meat into pieces;
2) Placing oyster meat into a pot, and cooking with small fire;
3) Stirring the cooked oyster meat into paste;
4) Pouring oyster mud into a filter screen, and washing with clear water to remove impurities;
5) Drying the filtered oyster mud;
6) Adding oyster peptidase into the dried oyster mud, performing enzymolysis to obtain oyster peptide, and drying again to obtain oyster peptide powder.
4. The method for preparing the oyster peptide and saussurea involucrata composition according to claim 1 or 2, wherein the method for preparing the saussurea involucrata active peptide powder is as follows:
1) Pulverizing dried herba Saussureae Involueratae into powder, adding appropriate amount of distilled water, soaking, and filtering;
2) Adding a proper amount of trypsin and papain into the collected filtrate, and carrying out enzymolysis at constant temperature;
3) Filtering the hydrolysate to remove residues and insoluble substances, and obtaining the active peptide filtrate containing the saussurea involucrata.
4) Concentrating and drying the filtrate to obtain the saussurea involucrata active peptide powder.
5. The method of preparing a oyster peptide and saussurea involucrata composition according to any one of claims 1-2, wherein the filtration is performed using a 50-150 mesh screen; preferably, the filtration is performed using a 100-150 mesh screen.
6. A method for preparing a oyster peptide and saussurea involucrata composition according to any of claims 1-3, wherein the concentration is atmospheric or vacuum.
7. A method of preparing an oyster peptide and saussurea involucrata composition according to any of claims 1-3, wherein the drying is spray drying or vacuum freeze drying.
8. An oyster peptide powder and saussurea involucrata active peptide composition, which is characterized by comprising oyster peptide powder and saussurea involucrata active peptide powder.
9. An oyster peptide powder and saussurea involucrata active peptide composition, characterized in that the composition is prepared by the method of claims 1-7.
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