CN116678875A - Alcohol degree detection method and device based on gold nanoparticle self-assembly system - Google Patents
Alcohol degree detection method and device based on gold nanoparticle self-assembly system Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 238
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 86
- 239000010931 gold Substances 0.000 title claims abstract description 86
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 86
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 85
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 238000001338 self-assembly Methods 0.000 title claims abstract description 22
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims abstract description 58
- 230000001476 alcoholic effect Effects 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 19
- 230000008859 change Effects 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims description 24
- 238000000926 separation method Methods 0.000 claims description 20
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 238000011068 loading method Methods 0.000 claims description 6
- 230000009467 reduction Effects 0.000 claims description 5
- 159000000001 potassium salts Chemical class 0.000 claims description 4
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 40
- 239000004323 potassium nitrate Substances 0.000 description 20
- 235000010333 potassium nitrate Nutrition 0.000 description 20
- 238000001125 extrusion Methods 0.000 description 6
- 230000009471 action Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 235000020097 white wine Nutrition 0.000 description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000012266 salt solution Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000001103 potassium chloride Substances 0.000 description 3
- 235000011164 potassium chloride Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 235000014101 wine Nutrition 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000003483 aging Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002077 nanosphere Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
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Abstract
An alcoholic strength detection method and device based on a gold nanoparticle self-assembly system, wherein the method comprises the following steps: respectively adding white spirit to be detected into a plurality of detection systems, wherein each detection system in the plurality of detection systems comprises gold nanoparticles and potassium salt, and the quality of the potassium salt in each detection system is different; after the white spirit to be detected is contacted with the detection system, the alcohol degree of the white spirit to be detected is judged based on the color change of a plurality of parts of the detection system. According to the application, the alcoholic strength range of the white spirit can be rapidly identified according to the color of one or more detection systems after white spirit to be detected is added, the detection time is usually about half a minute, and the white spirit detection method has the advantages of high sensitivity, simplicity in operation, low preparation cost and wide application value in market supervision of the white spirit.
Description
Technical Field
The application relates to the field of alcohol content detection, in particular to an alcohol content detection method and device based on a gold nanoparticle self-assembly system.
Background
The distilled spirit is a mainstream distilled spirit in the market, which is brewed by taking starter and yeast as saccharification starter and taking starchy or sugar as raw materials through the steps of steaming, saccharification, fermentation, distillation, ageing, blending and the like. The alcohol content is an important index in the process of producing and selling white spirit, and represents the volume percentage of ethanol in the white spirit. The alcoholic strength of the current commercial white spirit is mainly 18-78 degrees, and can be roughly classified into low degree, medium degree, high degree and extra high degree according to the alcoholic strength.
The alcoholic strength of the white spirit influences the taste of the white spirit, and the alcoholic strength detection of the white spirit is very important in production, sales and market supervision. The traditional method for detecting the alcoholic strength of the white spirit mainly comprises a specific gravity method, a chromatographic method, an infrared spectrometry method and the like. In recent years, some new alcohol detection methods have been increasingly popular, including alcohol detection test papers using fluorescent nanometers, and the like. However, these methods for detecting the alcoholic strength of white spirit have complicated detection steps and long waiting time of detection results, and are difficult to distinguish efficiently and in large quantities.
Disclosure of Invention
According to the method, based on research of selective self-assembly of gold nanoparticles by an inventor team, the characteristic that different colors are generated due to different one-dimensional linear assembly amounts of gold nanoparticles in ethanol and salt with different contents is utilized, and the alcoholic strength range of the current white spirit can be rapidly detected after white spirit with specific alcoholic strength is mixed with the gold nanoparticles and the salt, so that the alcoholic strength of the white spirit to be detected is efficiently, rapidly and largely determined.
The application is realized by the following technical scheme:
the alcoholic strength detection method based on the gold nanoparticle self-assembly system comprises the following steps:
respectively adding white spirit to be detected into a plurality of detection systems, wherein each detection system in the plurality of detection systems comprises gold nanoparticles and potassium salt, and the quality of the potassium salt in each detection system is different;
after the white spirit to be detected is contacted with the detection system, judging the alcohol degree of the white spirit to be detected based on the color change of a plurality of parts of detection systems;
wherein, the gold nanoparticles are synthesized by adopting a citric acid reduction method.
In the technical scheme, the gold nanoparticles are spherical gold nanoparticles synthesized by adopting the existing citric acid reduction method. Specifically, chloroauric acid can be added into boiling pure water, then sodium citrate is quickly added, the boiling stirring state is kept all the time by adjusting the heating temperature, the reaction is carried out for a period of time, for example, 20-30 minutes, when the color of the solution changes to wine red, the gold nanosphere particles are generated in the solution, the reaction is completed, and the initial product of the gold nanoparticles is obtained after the temperature is reduced. And then centrifuging, pouring out supernatant containing redundant byproducts, placing gold nanoparticles at the bottom into a new centrifuge tube, and adding pure water to fix the volume to obtain the gold nanoparticles.
In the technical scheme, each detection system comprises a certain amount of gold nano particles and potassium salt. The potassium salt may be potassium nitrate, potassium chloride or the like, and preferably the potassium salt is potassium nitrate. The mechanism of self-assembly of gold nanoparticles is: when gold nanoparticles take different ethanol contents as solvents, single gold nanoparticles are subjected to one-dimensional linear assembly under the drive of electrostatic acting force. When the number of gold nanoparticles in an assembled state is small and the chain length is short, the whole mixture presents red, and the whole color of the mixture is changed from red to wine red, purple, blue and deep blue along with the reduction of the number of single gold nanoparticles, the increase of the number of gold nanoparticles in the assembled state and the increase of the chain length.
Factors influencing the number of assembled states of gold nanoparticles include the ethanol content in alcohol and the potassium salt content in the system. Therefore, in the technical scheme, when the ethanol content in the white spirit to be detected is unknown, the white spirit is added into one or more detection systems, and as the gold nanoparticles in each detection system have the same content and the potassium salt content are different, after the white spirit is added, the different quality of the potassium salt in each detection system causes the same white spirit to be detected to display a specific color in different detection systems.
For example, when 38 degrees white spirit is added into 300 mu L of gold nano particles, 80 mu L of potassium nitrate and a detection system with the concentration of 0.5mol/L, the detection system is red after the white spirit is fully contacted with the detection system; when the volume of the potassium nitrate in the 38-degree white spirit in the added detection system is 100 mu L, the detection system is purple; when the volume of the potassium nitrate in the 38-degree white spirit in the added detection system is 120 mu L, the detection system is blue
Similarly, when the white spirit with 56 degrees is added into a detection system with the concentration of 0.5mol/L and the concentration of 300 mu L of gold nano particles and 80 mu L of potassium nitrate, the detection system is purple after the white spirit is fully contacted with the detection system; and when the volume of the potassium nitrate in the 56-degree white wine in the added detection system is 100 mu L and 120 mu L, the detection system is blue.
Based on the color development characteristic, in the technical scheme, a detection system can be adopted for judging, for example, whether the white spirit is low-degree white spirit, whether the white spirit is purple or not, whether the white spirit is medium-degree white spirit or not and whether the white spirit is blue or not can be judged by 300 mu L of gold nano particles, 80 mu L of potassium nitrate and a detection system with the concentration of 0.5 mol/L.
In the technical scheme, a plurality of detection systems can be combined to further improve the judgment accuracy. For example, two or more detection systems may be provided, where the potassium salt of each detection system has different quality, so that, after the white spirit to be detected is added, taking three detection systems as examples, the detection system group can present red-violet-blue, violet-blue distinction, thereby allowing the detection personnel to quickly and efficiently determine the alcohol content of the current white spirit.
According to the detection method, the alcoholic strength range of the white spirit can be rapidly identified according to the color of one or more detection systems after the white spirit to be detected is added, the detection time is usually about half a minute, the sensitivity is high, the operation is simple, the preparation cost is low, and the detection method has wide application value in market supervision of the white spirit.
Further, the molar ratio of the gold nanoparticles to the potassium salt is 0.04-0.02. In the technical scheme, if the ratio of the gold nanoparticles to the potassium salt is too high, the detection cost is increased, and the potassium salt in the system is insufficient to assemble the gold nanoparticles, so that the color change in the system is not obvious enough; conversely, if the ratio of the gold nanoparticles to the potassium salt is too low, the gold nanoparticles in the system are easier to assemble, and the detection system is easy to appear blue or deep blue, which is not beneficial to distinguishing the alcoholic strength range through the color. Therefore, in the present embodiment, the molar ratio of gold nanoparticles to potassium salt is 0.04 to 0.02, preferably, the molar ratio of gold nanoparticles to potassium salt is 0.0375 to 0.025.
Further, in the plurality of detection systems, the molar ratio of the detection system with the maximum potassium salt mass to the detection system with the minimum potassium salt mass is 1.2-2.0. When at least two detection systems are arranged, the potassium salt content of the two detection systems is not too close, otherwise, the difference of the colors of the detection systems is difficult to judge by naked eyes.
As a preferable setting mode of the detection system group in the application, the detection systems comprise three parts of detection systems, and the molar ratio of potassium salt in the three parts of detection systems is 4:5:6. In the technical scheme, the detection system group comprises three detection systems, the gold nanoparticles of the three detection systems have the same content, and the molar ratio of potassium salt is 4:5:6.
Further, the alcohol degree of the white spirit to be detected is judged based on the color change of a plurality of parts of detection systems within 120 seconds after the white spirit to be detected is contacted with the detection systems. With the increase of the contact time, the number of the transition of the single gold nanoparticles in the detection system to the assembled state is increased, and the overall color of the detection system tends to be deep blue, so that the judgment of the color is affected. Thus, in the present embodiment, the detection is preferably performed within two minutes, and further preferably, the detection is performed within one minute.
Further, the volume of the white spirit to be detected added into each detection system is 0.05-4.00 mL.
Another object of the present application is to provide a detection device based on any of the above methods for detecting alcoholicity based on a gold nanoparticle self-assembly system.
Specifically, the detection device comprises one or more detection units, the one or more detection units comprise gold nanoparticles and potassium salt, and the mass of the potassium salt in each detection unit is different.
In the technical scheme, one or more detection units included in the detection device form one or more detection systems in the detection method, each detection unit includes gold nanoparticles and potassium salt, and the quality of the potassium salt in different detection units is different. When the white spirit detection device is used, white spirit to be detected is respectively added into each detection unit, and the alcohol degree range of the white spirit to be detected can be identified by observing the color change condition of the detection system in the detection unit.
In practice, there are various arrangements of the detection means, which in some preferred embodiments are provided in the form of test plates. Specifically, detection device includes the bottom plate, be provided with a plurality of holding chamber on the bottom plate, hold the intracavity and be used for placing the detecting element, the detecting element is including the sample pad that loads gold nanoparticle and sylvite.
In the technical scheme, the detection system is solid before adding white spirit, and the carrying is more convenient. One or more accommodating cavities are arranged on the bottom plate of the detection device, and sample pads are arranged in the accommodating cavities. The sample pad is preferably a glass cellulose film, and the glass cellulose film is soaked in gold nanoparticle solution and potassium salt solution and then dried to obtain the sample pad loaded with gold nanoparticles and potassium salt.
When the white spirit to be detected is used, the white spirit to be detected is dripped into each accommodating cavity to react with gold nano particles and potassium salt on the sample pad in a dissolving way, and the alcoholic strength range of the white spirit to be detected is judged by observing the color change of the sample pad.
In some preferred embodiments, the detection device comprises a rack, a plurality of detection units are arranged on the rack, the detection units comprise centrifuge tubes, and gold nanoparticles and potassium salt are placed in the centrifuge tubes.
In the technical scheme, the detection system is liquid before adding the white spirit, and the detection system is in contact and uniformly mixed. The detection device comprises a placement frame, one or more detection units are arranged on the placement frame, and the detection units are centrifuge tubes filled with gold nanoparticle solution and potassium salt solution.
When the white spirit detection device is used, white spirit to be detected is dripped into each centrifuge tube, the color of the detection system is recorded after shaking, and the alcohol degree range of the white spirit to be detected is judged by means of one or more detection systems.
In a part of preferred embodiments, the detection device comprises a first plate and a second plate, the first plate and the second plate jointly form a plurality of cavities for placing the detection unit, the detection unit comprises a detection bottle, a separation membrane and a puncture member are arranged in the detection bottle, one side of the separation membrane is used for loading gold nanoparticles and sylvite, the other side of the separation membrane is used for loading white wine to be detected, and the puncture member is used for puncturing the separation membrane when the first plate and the second plate move towards each other.
In this technical scheme, detection device is including first board and the second board that can remove in opposite directions, all is provided with the recess of one-to-one on first board and the second board, and when first board and second board lock, the cavity that the recess formed is used for placing each detecting element.
In this technical scheme, detecting element is including detecting the bottle, is provided with the separation membrane in the detection bottle in order to divide into two parts with the inner space of detecting the bottle, and two parts all accessible lid and outside intercommunication. For example, one end of the detection bottle is a first cover body, an inner space between the first cover body and the separation film is used for placing gold nano particles and potassium salt solution, the other end of the detection bottle is a second cover body, and an inner space between the second cover body and the separation film is used for placing white spirit to be detected.
The first cover body and/or the second cover body are/is provided with the puncture piece, and the puncture piece can be pushed to move along the vertical direction by arranging the extrusion piece in the groove of the second plate under the action of external force, so that the separation membrane is pierced, the spaces on two sides of the separation membrane are communicated, and white spirit to be detected is in contact with gold nano particles and potassium salt solution to develop color.
When in use, the white spirit to be detected can be respectively placed in a plurality of detection bottles, and the quality of potassium salt in each detection bottle is different. During the process of moving the second plate and the first plate towards each other, the puncture member punctures the separating membrane under the action of the second plate, so that substances on two sides of the separating membrane start to contact at the same time and develop color gradually. The separation membrane realized by the puncture piece punctures simultaneously, and the detection system is mixed simultaneously, so that the accuracy of the detection result can be further improved.
Compared with the prior art, the application has the following advantages and beneficial effects:
1. according to the application, the alcoholic strength range of the white spirit can be rapidly identified according to the color of one or more detection systems after white spirit to be detected is added, the detection time is usually about half a minute, and the white spirit detection system has the advantages of high sensitivity, simplicity in operation, low preparation cost and wide application value in market supervision of the white spirit;
2. according to the application, the molar ratio of the gold nanoparticles to the potassium salt is 0.04-0.02, so that the detection cost is reduced, the color difference is improved, and the accuracy of judging the alcoholic strength range is further improved;
3. according to the application, by arranging the three detection systems, the molar ratio of the potassium salt in the three detection systems is 4:5:6, so that the detection system group can effectively distinguish the low-degree white spirit, the medium-degree white spirit and the high-degree white spirit, and no excessive detection systems are required, thereby improving the detection efficiency and the detection accuracy.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application. In the drawings:
FIG. 1 is a flow chart of an alcohol level detection method according to an embodiment of the present application;
FIG. 2 is a graph showing the ultraviolet absorbance of three different sets of detection systems after adding wine according to the embodiment of the application, wherein the abscissa is the wavelength and the ordinate is the absorbance;
FIG. 3 is a schematic diagram of an alcohol level detecting apparatus according to an embodiment of the present application;
FIG. 4 is a schematic diagram of another alcohol level detecting apparatus according to an embodiment of the present application;
FIG. 5 is a schematic diagram of an alcohol level detecting apparatus according to another embodiment of the present application;
FIG. 6 is a schematic view of a detecting bottle of another alcohol content detecting apparatus according to an embodiment of the present application;
FIG. 7 shows the color development of each detection system after adding white spirit with different alcohol degrees in the specific embodiment of the application.
In the drawings, the reference numerals and corresponding part names:
11-bottom plate, 12-holding chamber, 13-sample pad, 14-short column, 21-rack, 22-centrifuge tube, 31-first plate, 32-second plate, 33-extrusion, 4-detection bottle, 41-bottle, 42-first cover, 43-second cover, 44-moving member, 45-slider, 46-spring, 47-piercing member, 48-separation membrane.
Detailed Description
For the purpose of making apparent the objects, technical solutions and advantages of the present application, the present application will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present application and the descriptions thereof are for illustrating the present application only and are not to be construed as limiting the present application.
All the raw materials of the present application are not particularly limited in their sources, and can be commercially available or prepared according to conventional methods well known to those skilled in the art.
The purity of all the raw materials of the present application is not particularly limited, and the present application preferably employs purity requirements conventional in the field of analytical purity or alcohol detection.
All raw materials of the application, the brands and abbreviations of which belong to the conventional brands and abbreviations in the field of the related application are clear and definite, and the person skilled in the art can purchase from the market or prepare by the conventional method according to the brands, abbreviations and the corresponding application.
Example 1:
the alcoholic strength detection method based on the gold nanoparticle self-assembly system shown in fig. 1 comprises the following steps:
respectively adding white spirit to be detected into a plurality of detection systems, wherein each detection system in the plurality of detection systems comprises gold nanoparticles and potassium salt, and the quality of the potassium salt in each detection system is different;
after the white spirit to be detected is contacted with the detection system, judging the alcohol degree of the white spirit to be detected based on the color change of a plurality of parts of detection systems;
wherein, the gold nanoparticles are synthesized by adopting a citric acid reduction method.
In the technical scheme, as shown in fig. 2, a sharp absorption peak is arranged at about 520nm, the peak is an ultraviolet absorption peak of a single gold nanoparticle, when the gold nanoparticle starts to be assembled in a one-dimensional linear manner, an assembly peak appears at 650-700 nm, and the smaller the wavelength of the assembly peak, the shorter the chain is. Therefore, for the white spirit with 38 degrees, as the content of the added potassium nitrate increases, the number of gold nano particles in an assembled state increases, and the detection system correspondingly shows red (80 mu L of potassium nitrate), purple (100 mu L of potassium nitrate) and blue (120 mu L of potassium nitrate), so that the alcoholic strength range of the white spirit to be detected can be rapidly judged according to the color difference of the detection system.
In some embodiments, a detection system may be used to determine whether it is a low-alcohol white spirit, whether it is purple, whether it is a medium-alcohol white spirit, and whether it is blue, for example, by using a detection system of 300 μl gold nanoparticles, 80 μl potassium nitrate, and a concentration of 0.5 mol/L. In one or more embodiments, the alcoholic strength of the white spirit can be judged more accurately by setting a color chart.
In some embodiments, multiple detection systems may be used in combination to further improve the accuracy of the determination. For example, three detection systems can be provided, and the detection system group can display red-violet-blue, violet-blue and blue-blue distinction, so that detection personnel can quickly and efficiently judge the current alcohol degree of white spirit.
In a partially preferred embodiment, the molar ratio of gold nanoparticles to potassium salt is from 0.04 to 0.02, preferably the molar ratio of gold nanoparticles to potassium salt is from 0.0375 to 0.025.
In some embodiments, the molar ratio of the detection system with the highest potassium salt mass to the detection system with the lowest potassium salt mass in the plurality of detection systems is 1.2 to 2.0.
In some embodiments, the number of test systems includes three test systems, the molar ratio of potassium salts in the three test systems being 4:5:6. For example, in a detection system having a concentration of 0.5mol/L, the potassium salt of the detection system in triplicate is 80. Mu.L, 100. Mu.L and 120. Mu.L, respectively. The detection system group can effectively distinguish low-degree white spirit, medium-degree white spirit and high-degree white spirit, does not need to be provided with excessive detection systems, and improves the detection efficiency and the detection accuracy.
In some embodiments, the alcohol degree of the white spirit to be detected is determined based on the color change of a plurality of detection systems within 120 seconds after the white spirit to be detected contacts with the detection systems. Preferably, the detection is completed within 60 seconds.
In one or more embodiments, the volume of the white spirit to be detected added into each detection system is 0.05-4.00 mL.
In the embodiment, the alcoholic strength range of the white spirit can be rapidly identified according to the color of one or more detection systems after the white spirit to be detected is added, the detection time is usually about half a minute, and the white spirit detection device is high in sensitivity, simple to operate, low in preparation cost and wide in application value in market supervision of the white spirit.
Example 2:
on the basis of the embodiment 1, the alcoholic strength detection device based on the gold nanoparticle self-assembly system shown in fig. 3 comprises three detection units, wherein the three detection units comprise gold nanoparticles and potassium salt, and the quality of the potassium salt in each detection unit is different; the detection device comprises a bottom plate 11, a plurality of accommodating cavities 12 are arranged on the bottom plate 11, detection units are arranged in the accommodating cavities 12, and each detection unit comprises a sample pad 13 loaded with gold nanoparticles and potassium salts.
When the white spirit to be detected is used, the white spirit to be detected is dripped into each accommodating cavity to react with gold nano particles and potassium salt on the sample pad in a dissolving way, and the alcoholic strength range of the white spirit to be detected is judged by observing the color change of the sample pad.
In one or more embodiments, as shown in fig. 3, a plurality of short columns supported below the sample pad are further arranged in the accommodating cavity, so that enough space is provided below the sample pad to accommodate part of the white wine to be detected, overflow or sputtering out of the accommodating cavity after white wine is dripped into the accommodating cavity is avoided, the white wine is allowed to fully contact with the detecting system, and the color development effect is improved.
In some preferred embodiments, the device further comprises a dripping unit for white spirit to be detected, wherein the dripping unit can adopt a dropper, a pipette and other devices, so that the same adding amount of white spirit in each detecting unit is ensured as much as possible, and the accuracy of the result is improved.
In a part of the preferred embodiments, a timing unit is further included, and the timing unit is used for prompting that the comparison is completed within a specified time.
In some preferred embodiments, the system further comprises an acquisition unit, wherein the acquisition unit is used for acquiring pictures of detection results for subsequent data storage and comparison.
In some embodiments, the number of the detection units may be one or two, or may be more than four, and in one or more embodiments, one or more detection units may be used as a group, and multiple groups of detection units may be provided.
Example 3:
on the basis of the embodiment, the alcoholic strength detection device based on the gold nanoparticle self-assembly system shown in fig. 4 comprises a placement frame 21, wherein three detection units are arranged on the placement frame 21, each detection unit comprises a centrifuge tube 22, and gold nanoparticles and potassium salt are placed in the centrifuge tube 22.
When the white spirit detection device is used, white spirit to be detected is dripped into each centrifuge tube, the color of the detection system is recorded after shaking, and the alcohol degree range of the white spirit to be detected is judged by means of one or more detection systems.
In some embodiments, the number of the detection units may be one or two, or may be more than four, and in one or more embodiments, one or more detection units may be used as a group, and multiple groups of detection units may be provided.
Example 4:
on the basis of the above embodiment, the alcohol content detection device based on the gold nanoparticle self-assembly system as shown in fig. 5 and 6 comprises a first plate 31 and a second plate 32, wherein the first plate 31 and the second plate 32 jointly form three cavities for placing detection units, the detection units comprise detection bottles 4, separation membranes 48 and piercing elements 47 are arranged in the detection bottles 4, one side of each separation membrane 48 is used for loading gold nanoparticles and potassium salt, the other side is used for loading white spirit to be detected, and the piercing elements 47 are used for piercing the separation membranes 48 when the first plate 31 and the second plate 32 move towards each other.
When in use, the white spirit to be detected can be respectively placed in a plurality of detection bottles, and the quality of potassium salt in each detection bottle is different. During the process of moving the second plate and the first plate towards each other, the puncture member punctures the separating membrane under the action of the second plate, so that substances on two sides of the separating membrane start to contact at the same time and develop color gradually. The separation membrane realized by the puncture piece punctures simultaneously, and the detection system is mixed simultaneously, so that the accuracy of the detection result can be further improved.
In one or more embodiments, the first cover or the second cover is provided with a moving member, and the moving member is connected with a sliding block 45, where the sliding block 45 is located in a sliding groove on an inner wall of the first cover or the second cover, so that the moving member can vertically move up and down along the cover. The sliding block is also provided with a spring, when the sliding block is not extruded by the extrusion piece on the second plate, the moving piece keeps the initial position under the action of the spring, the penetrating piece does not penetrate through the separating membrane, and when the extrusion piece of the second plate extrudes the moving piece, the moving piece moves against the acting force of the spring, so that the penetrating piece penetrates through the separating membrane. When the extrusion part does not apply extrusion force to the moving part, the moving part is reset under the action of the spring, and the puncture part is removed from the puncture hole formed on the separation membrane, so that the liquid on two sides of the separation membrane can be fully contacted.
In some embodiments, the number of the detection units may be one or two, or may be more than four, and in one or more embodiments, one or more detection units may be used as a group, and multiple groups of detection units may be provided.
Example 5:
three groups of detection systems were set using the detection apparatus of example 3, wherein the gold nanoparticles in the three groups of detection systems were 1.5x10 -6 The concentration of the potassium nitrate is 0.5mol/L and the volumes are 80 mu respectivelyL, 100. Mu.L and 120. Mu.L, the contact time was 15 seconds.
As shown in fig. 7, after 38 degrees of white spirit was added, three groups of detection systems showed red (80 μl of potassium nitrate), purple (100 μl of potassium nitrate) and blue (120 μl of potassium nitrate), respectively; after addition of 56 degrees white spirit, the three groups of detection systems showed purple (80. Mu.L of potassium nitrate), blue (100. Mu.L of potassium nitrate) and blue (120. Mu.L of potassium nitrate), respectively. After adding 68 degrees white spirit, all three groups of detection systems are blue. Therefore, the alcoholic strength range of the white spirit to be detected can be judged within 15 seconds by combining the color development of different detection systems, and the white spirit detection device is high in sensitivity and simple to operate, and is beneficial to the market supervision department to carry out rapid real-time detection on the alcoholic strength of the white spirit.
The use of "first," "second," etc. (e.g., first panel, second panel, first cover, second cover, etc.) herein is for clarity of description only and is not intended to limit any order or emphasize importance, etc. to distinguish between corresponding components. In addition, the term "coupled" as used herein may be directly coupled or indirectly coupled via other components, unless otherwise indicated.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the application, and is not meant to limit the scope of the application, but to limit the application to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the application are intended to be included within the scope of the application.
Claims (10)
1. The alcoholic strength detection method based on the gold nanoparticle self-assembly system is characterized by comprising the following steps of:
respectively adding white spirit to be detected into a plurality of detection systems, wherein each detection system in the plurality of detection systems comprises gold nanoparticles and potassium salt, and the quality of the potassium salt in each detection system is different;
after the white spirit to be detected is contacted with the detection system, judging the alcohol degree of the white spirit to be detected based on the color change of a plurality of parts of detection systems;
wherein, the gold nanoparticles are synthesized by adopting a citric acid reduction method.
2. The method for detecting the alcoholic strength based on the gold nanoparticle self-assembly system according to claim 1, wherein the molar ratio of the gold nanoparticles to the potassium salt is 0.04-0.02.
3. The method for detecting the alcoholic strength based on the gold nanoparticle self-assembly system according to claim 1, wherein the molar ratio of the detection system with the largest potassium salt mass to the detection system with the smallest potassium salt mass in the plurality of detection systems is 1.2-2.0.
4. The method for detecting the alcoholic strength based on the gold nanoparticle self-assembly system according to claim 3, wherein the plurality of detection systems comprises three detection systems, and the molar ratio of potassium salts in the three detection systems is 4:5:6.
5. The method for detecting the alcohol degree based on the gold nanoparticle self-assembly system according to claim 1, wherein the alcohol degree of the white spirit to be detected is judged based on the color change of a plurality of detection systems within 120 seconds after the white spirit to be detected is contacted with the detection systems.
6. The method for detecting the alcoholic strength based on the gold nanoparticle self-assembly system according to claim 1, wherein the volume of the white spirit to be detected added into each detection system is 0.05-4.00 mL.
7. An alcoholic strength detection device based on a gold nanoparticle self-assembly system, characterized in that it is used in the detection method according to any one of claims 1 to 6, the detection device comprises one or more detection units comprising gold nanoparticles and potassium salt, and the mass of the potassium salt in each detection unit is different.
8. The alcoholic strength detection device based on the gold nanoparticle self-assembly system according to claim 7, comprising a bottom plate (11), wherein a plurality of accommodating cavities (12) are arranged on the bottom plate (11), detection units are arranged in the accommodating cavities (12), and the detection units comprise sample pads (13) loaded with gold nanoparticles and potassium salts.
9. The alcoholic strength detection device based on the gold nanoparticle self-assembly system according to claim 7, comprising a placement frame (21), wherein a plurality of detection units are arranged on the placement frame (21), each detection unit comprises a centrifuge tube (22), and gold nanoparticles and potassium salt are placed in the centrifuge tube (22).
10. The alcohol content detection device based on the gold nanoparticle self-assembly system according to claim 7, comprising a first plate (31) and a second plate (32), wherein the first plate (31) and the second plate (32) together form a plurality of cavities for placing detection units, the detection units comprise detection bottles (4), separation membranes (48) and piercing elements (47) are arranged in the detection bottles (4), one side of each separation membrane (48) is used for loading gold nanoparticles and potassium salt, the other side is used for loading white spirit to be detected, and the piercing elements (47) are used for piercing the separation membranes (48) when the first plate (31) and the second plate (32) move towards each other.
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| CN108107098A (en) * | 2018-01-30 | 2018-06-01 | 集美大学 | Based on WO3The method of alcoholic strength in/FTO photoelectric materials detection white wine |
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| CN112730399A (en) * | 2020-12-28 | 2021-04-30 | 泸州品创科技有限公司 | Method for rapidly detecting white spirit based on nano material-organic dye colorimetric sensor array |
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| CN108107098A (en) * | 2018-01-30 | 2018-06-01 | 集美大学 | Based on WO3The method of alcoholic strength in/FTO photoelectric materials detection white wine |
| CN110987915A (en) * | 2019-12-03 | 2020-04-10 | 泸州品创科技有限公司 | Method for rapidly identifying Chinese liquor based on gold nanorod colorimetric sensor array |
| CN111024689A (en) * | 2019-12-27 | 2020-04-17 | 华南理工大学 | White spirit alcoholic strength detection method based on color-changing nano material |
| CN112730399A (en) * | 2020-12-28 | 2021-04-30 | 泸州品创科技有限公司 | Method for rapidly detecting white spirit based on nano material-organic dye colorimetric sensor array |
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