CN116676195A

CN116676195A - Full-automatic suspension cell culture workstation

Info

Publication number: CN116676195A
Application number: CN202310864234.XA
Authority: CN
Inventors: 李建省; 孙永乐; 高铁锋; 邱波; 朱焱; 唐国伟
Original assignee: Youkang Biotechnology Beijing Co ltd
Current assignee: Youkang Biotechnology Beijing Co ltd
Priority date: 2023-07-14
Filing date: 2023-07-14
Publication date: 2023-09-01

Abstract

The invention provides a full-automatic suspension cell culture workstation which comprises a low-temperature box module, a coating module, a culture module and a control display module, wherein the low-temperature box module is used for storing a culture medium and controlling the adding type and weight of the culture medium; the coating module is used for performing cell coating and cell culture in the earlier stage; the culture module is used for carrying out cell culture; the control display module is used for controlling the whole cell culture process and displaying the current culture environment in real time. The cell culture workstation protected by the invention does not need manual intervention, and the cell culture process is automatically completed in a closed structure, so that the cell culture efficiency can be greatly improved.

Description

Full-automatic suspension cell culture workstation

Technical Field

The invention relates to the field of cell culture, in particular to a full-automatic suspension cell culture workstation.

Background

Cell culture, particularly of suspension cells (e.g., NK cells, 293, CHO, K562) used in immunocytotherapy, has been carried out essentially by artificial manipulation. For example, cells collected from a patient are inoculated together with a medium into a flask to which an antibody (an inducer) is attached, and then the flask is placed in a constant temperature bath, the flask is taken out of the constant temperature bath every day, a culture condition (for example, a proliferation condition) is observed using a microscope or the like, and when proliferation or the like is observed, or after a predetermined period of time has elapsed from the inoculation of the cells, the medium is added to the flask to culture (for example, proliferate) the cells.

Most of the existing automatic cell culture equipment integrates manual operation equipment in a laboratory into one large automatic culture equipment, and a series of operations are performed by using a mechanical arm instead of manpower.

However, most of the automated cell culture apparatuses are designed for the automatic operation of culture flask or for the automatic operation of bioreactor or microcarrier, and the operation apparatus is complicated and not suitable for individual cell culture.

Disclosure of Invention

In view of the technical problems set forth in the background art, the present invention provides a fully automatic suspension cell culture workstation for solving at least one technical problem set forth in the background art.

In order to achieve the above purpose, the present invention provides the following technical solutions:

a full-automatic suspension cell culture workstation comprises a low-temperature box module, a coating module, a culture module and a control display module, wherein,

the low-temperature box module is used for storing the culture medium and controlling the adding type and weight of the culture medium;

the coating module is used for performing cell coating and cell culture in the earlier stage;

the culture module is used for carrying out cell culture;

the control display module is used for controlling the whole cell culture process and displaying the current culture environment in real time.

As a further preferred embodiment, it further comprises an incubator module, said coating module and said culture module being arranged in the incubator module.

As a further preferred embodiment, the incubator module is peripherally provided with heating strips to control the temperature of the incubator module internal environment to 36-38 ℃.

As a further preferred embodiment, the incubator module is provided with an external air inlet through which it is connected to a carbon dioxide gas source and the concentration of carbon dioxide in the incubator module is controlled to be 4.9% -5.1%.

As a further preferable embodiment, the bottom of the incubator module is provided with a container for holding water, so as to control the humidity inside the incubator module to be more than 90%.

As a further preferred embodiment, the temperature of the internal environment of the incubator is in the range of 2-8 degrees celsius.

As a further preferred embodiment, a weighing sensor is arranged at the bottom of the low-temperature box module, and is used for collecting the weight of the culture medium in the low-temperature box module in real time.

As a further preferred embodiment, the cryostat module stores therein a plurality of media lines which merge into one line, and a first pinch valve is provided on each of the plurality of media lines prior to merging.

As a further preferable embodiment, the culture medium preheating device is arranged on the pipelines after the confluence and is used for heating the pipelines.

As a further preferred embodiment, the tubing heated by the medium preheating device is connected to the coating module by a first peristaltic pump for controlling the amount and/or rate of medium addition.

As a further preferable implementation scheme, the coating module is provided with a coating container, and a swinging motor is arranged to drive the coating container to swing.

As a further preferred embodiment, the coating module is provided with a vibration motor for driving the coating container to vibrate.

As a further preferred embodiment, a waste liquid recovery container is further arranged in the coating module, and a pipeline of the coating container is connected with the waste liquid recovery container through a second peristaltic pump.

As a further preferred embodiment, the culture module is provided with a swing table which drives the culture container on the swing table to swing left and right and/or up and down by motor drive.

As a further preferable embodiment, the swing table is provided with a cell density measuring device for measuring the density of the cultured cells in the culture container.

As a further preferable embodiment, the culture environment change displayed by the control display module includes at least one of a temperature curve, a humidity curve, a carbon dioxide concentration curve, a liquid feeding amount curve of the culture solution, and a cell density change curve.

As a further preferable embodiment, the control display module sets and/or alters the process flow through a display interface, including setting at least one of the execution time of each step, the liquid adding amount and liquid adding speed of the culture solution, and the amplitude angle and speed of the culture module swing table.

As a further preferable embodiment, the control display module can record and trace the historical data of the whole culture process, and view at least one of the temperature, the humidity and the carbon dioxide concentration in the culture process.

As a further preferred embodiment, the control display module is provided with a communication module, through which it can be connected with a mobile phone APP, remotely view workstation operation data and remotely control the workstation.

The suspension cell culture workstation protected by the invention adopts a fully-automatic closed system structure, and the whole culture process is not contacted with the outside, so that the risk of causing cell pollution is removed. The whole cell culture process is automatically completed without manual intervention. The whole cell culture process data is recorded in real time, and can be traced, inquired and alarmed. The cell growth density can be measured, and the culture medium can be supplemented according to time, and the culture efficiency is greatly improved.

Drawings

FIG. 1 is a schematic diagram of a fully automated suspension cell culture workstation according to one embodiment of the present invention;

FIG. 2 is a perspective view of a fully automated suspension cell culture workstation according to one embodiment of the present invention;

FIG. 3 is a perspective view of a coating module according to one embodiment of the present invention;

FIG. 4 is a partial perspective view of a coating module according to one embodiment of the present invention;

FIG. 5 is a partial perspective view of a vibration device in a coating module according to one embodiment of the present invention;

FIG. 6 is a perspective view of a culture module according to an embodiment of the invention;

FIG. 7 is another side perspective view of a culture module according to an embodiment of the invention;

FIG. 8 is a schematic view of a portion of a density sensor and culture vessel in a culture module according to an embodiment of the invention;

FIG. 9 is a schematic diagram showing the detection principle of the density sensor in the culture module according to an embodiment of the invention.

In the figure: 10-cell culture workstation, 1-low temperature box module, 101-first pinch valve, 102-first peristaltic pump, 2-coating module, 201-coating container, 202-primary cell container, 203-second pinch valve, 204-second peristaltic pump, 205-third pinch valve, 212-bracket, 213-spindle, 214-pin, 215-motor rod connector, 23-vibration device, 2301-vibration platform, 2302-vibration driving device, 24-driving motor, 25-driver, 26-fixing frame, 27-tray, 28 waste liquid bag, 3-culture module, 301-culture container, 3014-density detection port, 31-fixing plate, 312-bracket, 3121-first support spindle hole, 3122-U-shaped transverse support plate, 32-Y spindle bracket, 321-first swinging spindle, 322-connecting rod, 323-second support spindle hole, 33-density sensor, 35-X spindle swing, 351-second swinging spindle, 37-Y spindle motor, 371-first driving spindle, 38-motor, 39-X spindle, 39-sensor, 39-X spindle, 39-electronic control box, 4-display control module, 3101-first support spindle, 3-culture module, 4-electronic control box, display device, 4-electronic control box module, and display device.

Detailed Description

Features and exemplary embodiments of various aspects of the present invention are described in detail below, and in order to make the objectives, technical solutions, and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely configured to explain the present patent and are not configured to limit the present patent. It will be apparent to one skilled in the art that the present invention may be practiced without some of these specific details. The following description of the embodiments is merely intended to provide a better understanding of the present invention by showing examples of the present invention.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising … …" does not exclude the presence of other like elements in a process, method, article or apparatus that comprises the element.

Referring to FIG. 1, which is a block diagram of a fully automatic suspension cell culturing workstation according to the present invention, FIG. 2 is a perspective view of a cell culturing workstation, and a cell culturing workstation 10 includes a low temperature box module 1, a coating module 2, a culturing module 3 and a control display module 4. The above modules of the cell culture workstation 10 are described in detail below.

Firstly, the low-temperature box module 1 is used for storing various culture mediums in the low-temperature box module 1, and the temperature of the internal environment of the low-temperature box module is controlled to be 2-8 ℃ in order to keep the preservation quality of the culture mediums. Further preferably, a weighing sensor is arranged at the bottom of the low-temperature box module 1, so that the weight of the culture medium can be collected in real time, the weight of the used culture medium is further obtained, and the condition of adding the culture medium is known in real time. Further, since a plurality of media are stored in the low temperature box, each media pipe is provided with a first pinch valve 101, and the plurality of media pipes are finally combined into one pipe, and the combined pipes are connected to the coating module 2 to provide a proper amount of media for coating and culturing cells. The first pinch valve 1 disposed on each culture medium pipeline is connected with the control display module 4, and can control the opening or closing of any culture medium pipeline, thereby controlling which culture medium is added to the coating module 2 and the culture module 3.

In order to avoid the growth of image cells due to too low temperature of the culture medium, the culture medium is kept within a certain temperature range before the culture medium is added into the coating module 2, a culture medium preheating device 5 is arranged on a pipeline which is connected between the coating modules after the culture medium is converged, and the preheating device 5 heats the pipeline, so that the temperature of the added culture medium is ensured to be suitable for the requirement of cell growth.

Further, the preheated pipeline is connected to the coating device in the coating module 2 through a first peristaltic pump 102, and the first peristaltic pump 102 can be connected with a load cell in the low-temperature box module 1 and the control display module 4, so as to control the amount of the added culture medium and the speed of adding the culture medium.

In order to facilitate control of the temperature and humidity of the coating module 2 and the cultivation module 3 and the concentration of carbon dioxide, the coating module 2 and the cultivation module 3 are arranged in the incubator module 6, and the coating module 2 is supported above the cultivation module 3 through a bottom platform. Further, heating plates are arranged on the periphery of the incubator module 6, so that the temperature of the internal environment of the incubator module 6 is controlled to be 36-38 ℃, and the preferable temperature is 37 ℃. Further, the incubator block 6 is provided with an external air inlet port, the external air inlet port is connected to a carbon dioxide gas cylinder, and the concentration of carbon dioxide in the incubator block 6 is controlled to be 4.9% -5.1% by a valve, and the preferable concentration of carbon dioxide is 5%. Furthermore, a container for holding water, such as a basin, is placed on the bottom surface of the incubator module 6, water is added into the basin, and the humidity inside the incubator module is kept above 90%, preferably about 90%, by natural evaporation.

Further, the coating module 2 includes a coating container 201, a primary cell container 202, a waste liquid bag 28, a vibrating device 23, and a tray 27. The primary cell container 202 is placed above the coating module 2, and the primary cell container 202 is connected to the coating container 301 of the coating module 3 via a pipeline and a second pinch valve 203. When the primary cells need to be transferred into the coating vessel 201, the cells can automatically flow into the coating vessel 201 by opening the second pinch valve 203, so as to perform cell coating and early cell culture.

Further, in the primary cell culture coating process, the coating container 201 needs to be uniformly rocked and mixed, the coating module 2 is provided with a rocking table and a motor for driving the rocking table to rock, the coating container 201 is arranged on the rocking table, and the coating container 201 can be driven to rock left and right by the motor for driving the rocking table to improve the cell coating and culturing efficiency.

Further, referring to fig. 3 to 5, the coating module 2 of the present invention will be described in detail.

The swing table is a tray 27 capable of swinging. Further, in order to facilitate the fixation of the primary cell container 202, a holder 212 is further provided, and the primary cell container is provided on the holder 212. The primary cell container 202 and the bracket 212 thereof, the coating container 201, the vibrating device 23, the waste liquid bag 28 and the connecting pipelines thereof are all arranged on the tray 27, and the tray 27 can swing left and right under the drive of the tray driving device, and can also control the tray 27 to be in a horizontal position. The tray driving device includes a driving motor 24, a driver 25, a motor lever connector 215, a pin 214, and a rotation shaft 213. Referring to fig. 3, the rotating shaft 213 is disposed at a middle position on one side of the tray 27, the rotating shaft 213 is rotatably supported on the fixing frame 26 through a supporting member, the pin 214 is fixed on a side surface of the rotating shaft 213, the pin 214 is hinged with the motor connector 215, the motor connector 215 is disposed at an output end of the driving motor 24, and the driving motor 25 controls the motor 24 to enable the motor connector 215 to reciprocate up and down, so as to drive the rotating shaft 213 to reciprocate, and further drive the tray 27 to swing left and right. Preferably, the driving motor 24 is a push rod motor, and any one of an air pump cylinder, a voice coil motor, a reciprocating motor and a worm gear can be adopted for substitution, and the specific connection relationship is not repeated.

By means of the tray driving device, the driver 25 controls the motor 24 to select the corresponding tray 27 swing angle and amplitude level according to the cell type, so as to mix the cells fully.

Further, the coating vessel 201 is provided on the vibration device 23, and four corners of the cell coating vessel 201 are preferably fixed to the vibration device 23 by clips. The vibration device 23 includes a vibration table 2301 and a vibration driving device 2302 for driving the vibration table to vibrate, and the coating container 201 is fixed to the vibration table 2301. The vibration driving device 2302 is preferably an eccentric motor or an ultrasonic transducer, the eccentric motor can generate vibration with adjustable frequency, the ultrasonic transducer can generate mechanical waves, and by driving the vibration platform 2301 to generate vibration, the cells in the coating container 201 can be vibrated to be completely separated from the inner wall of the container, such as the cell coating bag wall/the cell coating box wall.

Further, the liquid outlet pipeline of the coating container 201 is connected to the waste liquid recovery container, namely, the waste liquid bag 28 through the second peristaltic pump 204, and when the waste liquid of the coating container 201 needs to be discharged, the second peristaltic pump 204 starts to work to pump the coating liquid and the cleaning liquid into the waste liquid recovery container. Further, referring to fig. 5, a waste bag 28 is provided below or at the bottom of the vibrating device 23, a cavity is provided below the vibrating device 23, and the waste bag 28 is placed in the cavity.

The culture vessel 301 of the culture module 3 is connected to the coating vessel 201 of the coating module 2 via a pipeline and the third pinch valve 205, and when cells are required to be transferred from the coating vessel 201 to the culture module 3 for culture after the cells are coated in the coating vessel 201 and the cells are cultured in the earlier stage, the third pinch valve 205 is opened, the coating module 2 is tilted downward and rightward, and the cells are transferred into the culture vessel by their own weight. Further, since the coating module 2 is further provided with the vibration device 23, when the cells in the coating container 201 need to be transferred into the culture container 301, the vibration motor 2302 of the vibration device 23 is turned on first, so that the coating container 201 vibrates, and then the cells attached to part of the coating container are separated, so that as many cells as possible are transferred out, and the cells are prevented from remaining in the coating container 201.

Further, referring to FIGS. 6 to 8, the culture module 3 of the present invention will be described in detail.

Further, the culture module 3 is provided with a swing table, the culture container 301 is arranged on the swing table, the swing table drives the culture container 301 to swing left and right and/or up and down through a motor, the swing motor of the swing table is connected with the control display module 6, the swing amplitude and speed can be set according to the requirement, substances secreted by cells are dispersed through swinging the culture container 301, and the cells also have sufficient space to absorb nutrition of the culture medium, so that the cells can grow rapidly.

Specifically, referring to fig. 6 and 7, the culture module 3 includes a fixing plate 31, a load cell 39, a Y-axis bracket 32, a Y-axis motor 37, an X-axis swing pallet 35, an X-axis motor 310, a culture container 301, a density sensor 33, and an electronic control board 38.

The weighing sensor 39 is disposed on the fixed plate 31, supporting frames 312 are disposed on two sides of the weighing sensor 39, a first supporting shaft hole 3121 is disposed at a position near the top of the supporting frames 312, the Y-axis support 32 is a rectangular frame, a first swinging shaft 321 is disposed at a middle position outside two long sides of the Y-axis support 32, the first swinging shaft 321 passes through the first supporting shaft hole 3121, and the Y-axis support 32 can rotate/swing relative to the supporting frames 312 by means of cooperation of the first swinging shaft 321 and the first supporting shaft hole 3121. Further, the first swinging shaft 321 at one side is hinged with one end of a connecting rod 322, the other end of the connecting rod 322 is hinged with a first driving rod 371 of a Y-axis motor 37, the Y-axis motor 37 is electrically connected with an electric control board 38, the Y-axis motor 37 drives the first driving rod 371 to do telescopic motion and drives the connecting rod 322 to swing, the swinging of the connecting rod 322 is converted into the rotation of the first swinging shaft 321, the left-right swinging of the Y-axis bracket 32 is further realized, the rotation amplitude and frequency of the Y-axis motor 37 can be controlled through a control board 38, and the swinging amplitude and frequency of the Y-axis bracket 32 are further controlled.

The X-axis swing supporting plate 35 is plate-shaped and basically similar to the shape of the Y-axis bracket 32 of the rectangular frame, but the whole size is slightly smaller than that of the Y-axis bracket 32, and the X-axis swing supporting plate 35 is arranged in the middle of the rectangular frame of the Y-axis bracket 32 and has gaps with the Y-axis bracket 32 at the periphery so as to ensure that the X-axis swing supporting plate 35 can swing in the Y-axis bracket 32. The second swing shafts 351 are disposed in the middle of the short sides of the two sides of the X-axis swing support plate 35, the second support shaft holes 323 are disposed at positions corresponding to the two short sides of the Y-axis support frame 32, the second swing shafts 351 on the two sides of the X-axis swing support plate 35 penetrate into the second support shaft holes 323 in the middle of the two short sides of the Y-axis support frame 32 respectively, and the X-axis swing support plate 35 also rotates/swings relative to the Y-axis support frame 32 by means of the cooperation of the second swing shafts 351 and the second support shaft holes 323, preferably, the second swing shafts 351 and the second support shaft holes 323 are disposed on one side only. It is further preferable that a U-shaped lateral support plate 3122 is provided to extend downward at both sides of the Y-axis bracket 32 (i.e., at the position of the first swing axis 321), and the X-axis motor 310 is provided at a position near to the one-side support frame 312 on the lateral plate of the U-shaped lateral support plate 3122, that is, the X-axis motor 310 is located at a distance from the center of the lateral support plate 3122. The output end of the X-axis motor 310 is connected with a second driving rod 3101, the other end of the second driving rod 3101 is hinged to the X-axis swinging support plate 35, the hinged position deviates from the axes of the second swinging shafts 351 at two sides by a certain distance, so that the second driving rod 3101 is pushed to perform telescopic motion under the driving of the X-axis motor 310, and then the X-axis swinging support plate 35 can swing around the second swinging shafts 351, preferably, the X-axis motor 310 is electrically connected with the electric control plate 38, and the rotation amplitude and frequency of the X-axis motor 310 can be controlled through the control plate 38, and further the swinging amplitude and frequency of the X-axis swinging support plate 35 are controlled.

Because the low-temperature box module 1, the coating module 2 and the culture module 3 are connected through the pipelines, and the first pinch valve 101, the third pinch valve 205 and the first peristaltic pump 102 are arranged on the pipelines, when the culture medium is required to be added in the cell culture process, the first pinch valve 101 of the culture medium is opened, the first peristaltic pump 102 is opened, the third pinch valve 205 of the culture container is opened, and the culture medium flowing out of the low-temperature box module 1 can flow into the culture container 301 through the coating module 2, so that the addition of the culture medium is completed.

Further, it is necessary to detect the growth of cells during the cell culture, and the culture vessel 301 is made of FEP material having good light transmission and air permeability. Further, a cell density measuring device, specifically a density sensor 33, is disposed on the swing table of the culture module 3, and includes an infrared light source, a reference infrared receiver, a detection infrared receiver, and an optical signal processing circuit.

The culture container 301 is fixed on a swing table, a density detection port 3014 is arranged at the side edge of the culture container 301, the density detection port 3014 can be inserted into a cell measurement device, an infrared light source of the cell measurement device emits light with fixed wavelength, such as 800-1100nm infrared parallel light, infrared light transmits cells in the cell culture bag density detection port, absorbance is calculated by comparing the infrared light intensity received by an infrared receiver after the cells are transmitted with the infrared light intensity when no liquid exists in the cell culture bag density detection port, and then the density of the cultured cells is calculated. Further preferably, the density detection port 3014 on the side of the culture vessel 301 is welded to the edge of the culture vessel 301, and the inside of the density detection port 3014 communicates with the space in the culture vessel 301, so that the liquid in the culture vessel 301 can flow into the inside of the density detection port 3014 when the swing table swings. The density sensor 33 is provided on the X-axis swing pallet 35, and the density detection port 3014 can be inserted into the density sensor 33 when the culture container 301 is fixed to the X-axis swing pallet 35. Preferably, the density sensor 33 is provided at one corner of the X-axis swing pallet 35.

The structure and principle of the density sensor 3 is shown in figures 8 and 9,

1. an infrared light source of 800-1100nm is adopted to provide a stable and reliable parallel light source, and the light of the wave band has extremely high absorptivity to the measured object, so that the absorptivity to the infrared light of the measured object can be conveniently detected.

2. Parallel rays of the infrared light source pass through the 1:1 beam splitter prism to divide the light source into two parts, and the two parts are respectively provided as a reference light source and a detection light source for detection and light source intensity correction.

3. And the reference infrared receiver A is used for monitoring the stability of the light source in real time and providing a correction basis for the subsequent calculation of the change of the incident light intensity value.

4. The density detection port 3014 is welded to the edge of the culture container 301, and the inside of the density detection port 3014 communicates with the space in the culture container 301, so that the liquid in the culture container 301 can flow into the inside of the density detection port 3014 when the swing table swings. Preferably, the detection portion of the density detection port 3014 is highly transparent and can transmit light.

5. Through 1: the parallel light after the beam splitter 1 passes through the density detection port 3014, and the parallel light absorbed by the detected object enters the detection infrared receiver B.

6. The reference parallel light signal received by the reference infrared receiver A and the absorbed parallel light signal received by the detection infrared receiver B enter, the illumination values obtained by the reference infrared receiver A and the detection infrared receiver B are compared and corrected, the incident light intensity and the received light intensity at the moment are calculated, the absorbance value is calculated, the density of cells is represented, and the density value change of an object to be detected is indirectly detected.

The control display module 4 is a control center of the whole full-automatic suspension cell culture workstation and is responsible for displaying the whole cell culture flow and the current culture environment changes such as a temperature curve, a humidity curve, a carbon dioxide concentration curve, a liquid adding amount curve, a cell density change curve and the like in real time. Further, the culture process and flow can be set/changed through a display interface, for example, the execution time length, the liquid adding amount, the liquid adding speed of each step, the swing amplitude angle and the swing speed of the culture module swing table, and the like are set. Each control point in the culture process can be modified at any time in an editable mode of a table format, so that different cell culture processes can be conveniently searched, and the individual culture requirements of cell culture are met.

Furthermore, the control display module 4 can record and trace the history data of the whole culture process, and check the parameter changes such as temperature, humidity, carbon dioxide concentration and the like in the culture process. More preferably, the control display module is further provided with a communication module, the data is uploaded to the cloud after networking, the mobile phone can communicate with the control display module, and the mobile phone APP can remotely check the operation data of the workstation and remotely control the workstation.

The cell culture workstation protected by the invention adopts a fully-automatic closed system structure, and the whole culture process does not have any contact with the outside, so that the risk of causing cell pollution is removed. The whole cell culture process is automatically completed without manual intervention. The whole cell culture process data is recorded in real time, and can be traced, inquired and alarmed. The cell growth density can be measured, and the culture medium can be supplemented according to time, and the culture efficiency is greatly improved.

It will be apparent to those skilled in the art that various modifications and variations can be made to the embodiments of the present invention without departing from the spirit and scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims

1. A full-automatic suspension cell culture workstation is characterized by comprising a low-temperature box module, a coating module, a culture module and a control display module, wherein,

the culture module is used for carrying out cell culture;

2. The fully automated suspension cell culture workstation of claim 1 further comprising an incubator module, the coating module and the culture module being disposed in the incubator module.

3. The fully automated suspension cell culture workstation of claim 2 wherein the incubator module is peripherally provided with heating strips to control the temperature of the incubator module internal environment to 36-38 degrees celsius.

4. The fully automated suspension cell culture workstation of claim 2 wherein the incubator module is provided with an external air inlet through which it is connected to a carbon dioxide source and controls the carbon dioxide concentration within the incubator module to be 4.9% -5.1%.

5. The fully automatic suspension cell culture workstation of claim 2 wherein a container for holding water is provided at the bottom of the incubator module to control the humidity within the incubator module to be above 90%.

6. The fully automated suspension cell culture workstation of claim 1 wherein the temperature of the internal environment of the incubator is between 2-8 degrees celsius.

7. The fully automatic suspension cell culture workstation of claim 1 wherein a load cell is disposed at the bottom of the low temperature tank module for real-time collection of the weight of the culture medium in the low temperature tank module.

8. The fully automated suspension cell culture workstation of claim 1 wherein the low temperature tank module stores multiple media therein, the multiple media lines merge into one line, and a first pinch valve is provided on each of the multiple media lines prior to merging.

9. The fully automated suspension cell culture workstation of claim 8 further comprising a culture medium preheating device disposed in the joined lines for heating the lines.

10. The fully automatic suspension cell culture workstation of claim 9 wherein the tubing heated by the culture medium preheating device is connected to the coating module by a first peristaltic pump for controlling the amount and/or rate of medium addition.

11. The fully automatic suspension cell culture workstation of claim 1, wherein the coating module is provided with a coating container, and a swinging motor is arranged to drive the coating container to swing.

12. The fully automatic suspension cell culture workstation of claim 11, wherein the coating module is provided with a vibration motor for driving the coating container to vibrate.

13. The fully automatic suspension cell culture workstation of claim 11, wherein the coating module is further provided with a waste liquid recovery container, and the pipeline of the coating container is connected with the waste liquid recovery container through a second peristaltic pump.

14. The fully automatic suspension cell culture workstation according to claim 1, wherein the culture module is provided with a swing table, and the swing table drives the culture container on the swing table to swing left and right and/or up and down through motor drive.

15. The fully automated suspension cell culture workstation of claim 14 wherein the swing table is provided with a cell density measurement device for measuring the density of the cultured cells in the culture vessel.

16. The fully automated suspension cell culture workstation of any of claims 1-15 wherein the culture environment change displayed by the control display module comprises at least one of a temperature profile, a humidity profile, a carbon dioxide concentration profile, a broth charge profile, and a cell density change profile.

17. The fully automated suspension cell culture workstation of any one of claims 1-15 wherein the control display module sets and/or alters a process flow via a display interface, including setting and/or altering at least one of a length of time each step is performed, a rate of addition and a volume of culture fluid, and an amplitude angle and a rate of a culture module swing.

18. The fully automated suspension cell culture workstation of any one of claims 1-15 wherein the control display module is capable of recording and tracking historical data throughout the culture process and viewing at least one of temperature, humidity, and carbon dioxide concentration during the culture process.

19. The fully automatic suspension cell culture workstation of claim 18 wherein the control display module is provided with a communication module through which it can be connected to a cell phone APP to remotely view workstation operating data and remotely manipulate the workstation.