CN116650849B

CN116650849B - Special fixing protection device for mouse radiotherapy and use method

Info

Publication number: CN116650849B
Application number: CN202310541162.5A
Authority: CN
Inventors: 徐小洁; 杜祎萌; 梁磊; 亢小峰
Original assignee: Academy of Military Medical Sciences AMMS of PLA
Current assignee: Academy of Military Medical Sciences AMMS of PLA
Priority date: 2023-05-15
Filing date: 2023-05-15
Publication date: 2024-03-22
Anticipated expiration: 2043-05-15
Also published as: CN116650849A

Abstract

The invention relates to the field of instruments for supporting or binding animals for surgical purposes, in particular to a special fixing and protecting device for mouse radiotherapy and a use method thereof, wherein the special fixing and protecting device comprises a bearing plate, a mouse fixing plate and a protecting baffle matched with the mouse fixing plate, wherein the mouse fixing plate is arranged on the bearing plate and is used for placing experimental mice; marking a central axis on the upper surface of the mouse fixing plate, arranging a tumor placement area and an abdominal anesthesia area beside the central axis, and covering a radiotherapy rubber cushion on the body of the experimental mouse; the protective baffle has two sets and every group is by the overhead gage and the side baffle that forms through the welding, and the overhead gage surface is opened there is the irradiation window, and two lead pieces that the irradiation window surface is placed the size unanimity. The invention solves the defects that the prior device is difficult to carry out chemotherapy experiment operation, does not have a matched protective baffle, lacks an effective fixing system and the like, improves the flexibility of adjusting the size of an irradiation window, is convenient for midway operation and supplement, and is easier for observing mice.

Description

Special fixing protection device for mouse radiotherapy and use method

Technical Field

The invention relates to the field of instruments for supporting or binding animals for surgical purposes, in particular to a special fixing and protecting device for mouse radiotherapy and a using method thereof.

Background

Tumor-bearing experimental animals are important for clinical research of cancers, and are widely applied in the fields of drug development and the like. There are a variety of tumor-bearing animal models applied in research, of which the simplest and most widely used are murine or humanized tumor models grown under the skin of mice, and such mice are called subcutaneous tumor-bearing mice. In preclinical studies of radiotherapy-sensitized anticancer drugs, researchers must use subcutaneous tumor-bearing mice to conduct radiotherapy-related experiments. In order to ensure the successful implementation of such experiments, the following 5 basic conditions need to be met: (1) good anesthesia; (2) proper fixation of the mice after anesthesia; (3) the tumor is substantially exposed to radiation; (4) good protection of normal organs; (5) minimizing experimental errors. In practical studies, the devices that provide radiation therapy to mice are often large devices for clinical treatment, and these radiation therapy devices are not equipped with the associated equipment dedicated to the experiments of mice. Therefore, to satisfy the above 5 basic conditions, it is necessary to use an experimental apparatus suitable for mice with perfect and reasonable design. To achieve this, devices that effectively block radiation while adequately exposing the tumor, known as shielding plates, must be used in radiotherapy experiments.

In the prior art, the device for fixing the mice is not special for the radiotherapy experiment of the mice. For example, plastic mouse fixing barrels most commonly used in laboratories are commonly used for fixing when blood is collected from the tail vein or administered via the tail vein. In the recent related inventions, there is no fixation device dedicated to the radiation therapy of mice either.

Lu Yongzhou et al, discloses a portable mouse fixing clip (publication No. CN 204863544U), which is mainly used for the operation of skin or subcutaneous tissue on the back of a mouse, and is matched with a mouse cage cover to be used for completing the operation of the back of the mouse when operated by a single person. However, its application in mouse radiotherapy experiments has the following problems: (1) The fixing function of the device is realized through the joint connection of the squirrel cage cover and the fixing clamp, and the squirrel cage cannot be placed on a radiotherapy operation bed in actual work. (2) The cage cover is space-efficient and has a limited number of mice that can be fixed at one time. If the device is used for batch radiotherapy experiments, the working efficiency can be seriously affected. (3) If the mouse cage cover is not matched, the mouse clamp is independently used for fixing, and good fixing of the mouse is difficult to ensure due to the structural characteristics of an I shape. (4) is not suitable for immobilization of tumor-bearing mice on the abdominal wall. (5) The required operation steps are complicated, and the processing technology is complicated. (6) There is no protective barrier that can be used with the device.

Yu Miao et al, "two-in-one tumor-bearing mouse inguinal subcutaneous tumor measurement fixator" (publication No. CN 213190294U), is mainly used for performing related operations of tumor-bearing mouse inguinal subcutaneous tumor measurement.

At the same time, few protective barriers are available for mice. In the recent related invention, there are only 1 such devices, namely, the "protection baffle for tumor-bearing mice" designed by the star of the river (publication number: CN 212522742U) is a design based on a fixed cylinder of mice, and although the device can partially solve the protection problem in the radiotherapy experiment of mice, the following problems exist in the practical work due to the defects of the design: (1) The device both ends opening is difficult to guarantee that the mouse is motionless when anesthesia degree of depth is mastered poorly, very easily makes the tumour be shifted out the irradiation window, and the mouse will escape the device when serious. In addition, because the device has no fixed part inside, the mouse is easy to move in the barrel when the operator puts and moves the device, so that the tumor is moved out of the irradiation window. (2) While the use of a mating "aperture sheath" allows for adjustment of the area of the illumination window, it lacks better flexibility in adjusting the illumination window due to the design characteristics of the circular aperture and the limited size of the aperture sheath. This design does not necessarily match the tumor well, that is to say there is a problem with too much or too little occlusion. (3) Although the device has the function of blocking rays, a lead barrel which is completely opaque and a hole sheath with a small inner diameter can seriously block rays, and the design makes it difficult for an operator to observe and position tumors in an irradiation window. (4) The cylindrical structure makes the radiotherapy cushion for radiotherapy unable to cover on the mouse. (5) When the operation such as additional anesthesia is required due to the shielding of the lead barrel, the mice must be pulled out of the barrel, and then the operations such as placement and alignment are required, which significantly affects the working efficiency. (6) The cylindrical design is prone to rolling and shifting on a flat radiotherapy bed. (7) The design has no measuring and positioning system, and is unfavorable for the standardization of experiments. (8) machining the sheath of holes requires a relatively fine process.

In summary, the problems in the prior art mainly include: (1) the fixation device is not suitable for use in mouse radiation therapy; (2) no matched protective baffle is arranged on the existing fixing device; (3) The existing protective baffle lacks an effective fixing system, the size of the irradiation window is not flexibly adjusted, the radiation therapy rubber cushion cannot be covered, the supplementary midway operation is inconvenient, and the observation of a mouse is not facilitated; (4) The special radiotherapy fixing and protecting device for the tumor-bearing mice is a key device for carrying out radiotherapy experiments on the tumor-bearing mice, and the existing related products are few, and the requirements of actual scientific research work are hardly met.

Disclosure of Invention

The invention aims to provide a special fixing and protecting device for mouse radiotherapy and a using method thereof, which are used for solving the defects that the conventional device is difficult to carry out chemotherapy experiment operation, does not have a matched protecting baffle, lacks an effective fixing system and the like, and simultaneously improving the flexibility of adjusting the size of an irradiation window, facilitating midway operation and supplement and being easier for observing a mouse. In addition, it can also cover the radiotherapy cushion, protects the mouse better.

In order to achieve the above purpose, the present invention provides the following technical solutions:

the invention provides a special fixing and protecting device for mouse radiotherapy, which comprises a bearing plate, a radiotherapy rubber mat, a mouse fixing plate for fixing an experimental mouse and a protecting baffle matched with the mouse fixing plate;

the mouse fixing plate is arranged on the bearing plate, the upper surface of the mouse fixing plate is marked with a central axis, and a tumor placement area and an abdominal anesthesia area are arranged beside the central axis;

the two groups of protective baffles are formed by welding an upper baffle and two side baffles, and a space formed below the protective baffles is used for accommodating a mouse fixing plate, an experimental mouse and a radiotherapy rubber pad;

an irradiation window is arranged on the surface of the upper baffle plate, two lead blocks with the length and the width of 4cm are placed on the surface of the irradiation window, and the area of the irradiation window can be flexibly adjusted according to the size of a tumor by covering the lead blocks on the edge of the irradiation window;

the upper baffle plate and the side baffle plates are made of lead materials.

Further, the mouse fixing plate is a cuboid with the size of 15cm by 7cm, and is made of foam plastic, and the plate thickness is designed to be 2.5cm.

Further, the experimental mice were fixed on the mice fixing plate by a binding, which comprises two parts of an upper fixing tape and a lower fixing tape and is transparent plastic tape.

Further, two groups of four mouse fixing plates are arranged up and down along the length direction of the plates and then are matched with the size of the radiotherapy machine.

Further, the radiotherapy rubber pad is 30cm by 1.0cm.

Further, the thicknesses of the upper baffle and the side baffles are 5mm, and the heights of the side baffles are 7cm.

Further, the surface of each upper baffle plate is provided with 4 square irradiation windows with equal distance and side length of 2.5cm.

Furthermore, an alignment reference line is arranged beside each irradiation window, and a sticky graduated scale is arranged on the edge of each irradiation window.

The invention also provides a use method of the special fixing and protecting device for the radiation treatment of the mice, which is characterized in that: firstly, placing the body of an experimental mouse at the positions corresponding to the tumor placement area and the abdominal anesthesia area, then fixing the trunk, the rear legs and the other part of the tail of the experimental mouse on the mouse fixing plate through the upper fixing adhesive tape and the lower fixing adhesive tape respectively, then covering the radiotherapy rubber mat on the experimental mouse, placing the protective baffle above the mouse fixing plate, the experimental mouse and the radiotherapy rubber mat, then adjusting the irradiation window to carry out radiotherapy irradiation, returning the experimental mouse to the original laboratory after the radiotherapy is finished, and finally dismantling and fixing.

Compared with the prior art, the invention has the beneficial effects that:

1. the scheme of combining the mouse fixing plate with the radiotherapy protective baffle fills the blank that no set of special fixing and protective device exists in the mouse radiotherapy experimental equipment.

2. The mouse fixing plate is made of foamed plastic with the thickness of 2.5cm, so that the mouse fixing plate can be ensured to have enough strength and is not easy to break in use. The material has the advantages of low price, moderate elasticity, difficult temperature severe change along with air temperature, light weight and the like. Compared with wooden and hard plastic boards, the mouse fixing board made of foamed plastic has better comfort and transportation convenience. In addition, with the use of the mouse fixing plate made of such a material, the possibility of accidental injury of the mouse during transportation is also smaller. Compared with the integral mouse fixing plate of a plurality of mice, the split type design is more flexible, and the split type design is convenient to be matched with the protective baffle better.

3. The central axis of the mouse fixing plate is marked to be used as a mark for placing the body of the mouse, the tumor is placed in the tumor placement area, and the natural central axis is ensured to be overlapped with the central axis of the mouse fixing plate.

4. The mouse fixing plate adopted by the design is very suitable for placing the body position during inoculation of subcutaneous tumors, so that the mouse can more easily adopt a prone position or a supine position. The design effectively shortens the operation time, reduces the operation difficulty and improves the stability and accuracy of the experiment. At the same time, for mice, the more comfortable inoculum sites will also alleviate their stress and pain sensation, contributing to the welfare assurance of the animals.

5. Through the design of the abdominal anesthesia area, the experimenter can directly perform the intraperitoneal injection anesthesia without detaching the fixing device, which obviously reduces the time required by the experiment, greatly saves the operation time and is convenient for the operator.

6. Through using the clear plastic tape of 6cm wide, firmly paste the skin of trunk and hind leg of mouse and fixed plate together to assist 3cm wide clear plastic tape and firmly paste the rat tail on the fixed plate, satisfied the requirement of fixed mouse: safe and harmless, reliable fixation, convenient operation and observation, convenient marking and convenient midway additional fixation. The fixing mode has the advantages that the mouse can be fixed simply and easily, and the traumas and the pressure on the mouse caused by the traditional fixing mode adopting surgical suture are avoided. Meanwhile, the mouse can be fixed by using the transparent plastic adhesive tape, so that the movement condition of the mouse can be observed better, and the mouse can be adjusted or additionally fixed more conveniently if necessary. The implementation of the design is helpful for improving the efficiency and accuracy of the experiment, and meanwhile, pays more attention to welfare guarantee of the mouse experiment.

7. By fully considering the species, the tumor position and the tumor volume of the mice and tightly combining the actual working requirements and the ethical requirements of animals, the whole device has wider application range and more practical application value.

8. The protective baffle can be conveniently and stably arranged on the bearing plate of the radiotherapy bed by welding the protective lead side baffle with the height of 7cm.

9. The protective baffle can well protect normal organs of the experimental mice, avoid accidental death of the experimental mice, and provide firm guarantee for smoothly carrying out subsequent experimental contents such as survival time analysis, pharmacodynamics analysis, pathology analysis and the like.

10. The space formed below the protective baffle is enlarged to be used for accommodating the mouse fixing plate, the mouse and the radiotherapy rubber pad, so that sufficient operation and observation space can be reserved for researchers, and the standardization and the precision of experiments can be improved.

11. The device with simple design is formed by using cheap and easily obtained materials (the plastic foam board, the transparent adhesive tape and the lead board), so that the mass industrial production is very easy to realize, and the cost of experiments is greatly reduced.

Drawings

FIG. 1 is a schematic view of an application scenario of the present invention;

FIG. 2 is a top view of the protective device of the present invention;

FIG. 3 is a top view of the upper baffle of the guard of the present invention;

FIG. 4 is a top view of various abdominal anesthesia zones of the present invention;

FIG. 5 is a top view of the whole coverage radiotherapy of the mice;

FIG. 6 is a flow chart of experimental operation of the present invention;

fig. 7 is a view of an example usage scenario of the present invention.

In the figure: 10-a carrier plate; 20-a protective baffle; 30-central axis; 40-binding; 100-mouse fixation plate; 101-tumor placement region; 102-an abdominal anesthesia zone; 110-experimental mice; 111-fixing the adhesive tape above; 112-fixing adhesive tape below; 120-radiotherapy rubber pad; 200-side baffles; 210-upper baffle; 220-illumination window; 221-a stickable scale; 222-a para-reference line; 230-lead block.

Detailed Description

In order that the manner in which the above recited features, objects and advantages of the present invention are obtained will become readily apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings. Based on the examples in the implementation manner, other examples obtained by a person skilled in the art without making creative efforts fall within the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.

Referring to fig. 1-7, the invention discloses a special fixing and protecting device for mouse radiotherapy and a using method thereof, wherein the special fixing and protecting device comprises a bearing plate 10, a mouse fixing plate 100 and a protecting baffle 20 matched with the same, wherein the mouse fixing plate 100 is arranged on the bearing plate and is used for placing experimental mice; the upper surface of the mouse fixing plate 100 is marked with a central axis 30, a tumor placement area 101 and an abdominal anesthesia area 102 are arranged beside the central axis, and the body of the experimental mouse 110 is covered with a radiotherapy rubber pad 120; the protective baffle 20 has two groups, each group is composed of an upper baffle 210 and a side baffle 200 formed by welding, the space formed below the protective baffle 20 is used for accommodating a mouse fixing plate 100, an experimental mouse 110 and a radiotherapy rubber pad 120, an irradiation window 220 with a sticky graduated scale 221 and an alignment reference line 222 aligned with the mouse fixing plate 100 are arranged on the surface of the upper baffle 210, and two lead blocks 230 with the same size are placed on the surface of the irradiation window.

In this embodiment, the radiotherapy system of the SHINVAXHA-1400 medical electron linear accelerator is used as a working platform, and the mouse fixing plate and the protective baffle 20 are designed in a matching way according to the sizes of all parts of the platform, the body length of the mouse and the tumor size allowed by animal ethics welfare. By the mating design, the fixture and the protective barrier 20 are perfectly matched. Simultaneously, this design make full use of the space of radiotherapy bed bearing plate, once operate can provide radiation protection simultaneously for 8 mice at most.

Referring to fig. 6-7, the following flow is used in the specific embodiment:

s1: subcutaneous inoculation of tumor cells

A tumor cell line and the site of inoculation are selected according to the experimental requirements and inoculated subcutaneously.

S2: mouse feeding

After tumor inoculation, mice were bred and observed according to breeding codes until tumor volumes grew to the size required by the experimental design.

S3: skin preparation

Skin preparation was performed for mice 1 day prior to irradiation. Taking a waist or leg subcutaneous tumor-bearing mouse as an example, the specific method comprises the following steps: the hair from the neck to the back of the tail of the mouse is shaved by an electric shaver, and then the hair is removed by using a depilatory cream. Skin preparation is very important, and good exposure to the skin can ensure firm adhesion.

S4: anesthesia

The mice are generally anesthetized by intraperitoneal injection of anesthetic drugs.

S5: selecting fixing plate

After completion of the abdominal anesthesia, a mouse fixing plate 100 matching the tumor inoculation area was selected. A clear plastic tape of a suitable length is prepared.

Specifically: corresponding data are obtained by measuring the dimensions of the radiotherapy bed board, the loading plate and the radiotherapy rubber pad 120. Radiation treatment bed plate width: 48cm; load-bearing plate: 40cm x 40cm; radiotherapy cushion 120:30cm by 1.0cm. Body length (length of mouse mouth-nose to tail-root) and body width (length of the widest part of the body of the mouse 110) data of the mouse 110 were obtained by measuring the mouse 110 at 5-7 weeks of age: the mice 110 had an average body length of about 8cm to 9cm and an average body width of about 4cm.

On the premise of fully utilizing the space of the bearing plate and the tissue compensation pad, in order to make the mice 110 more comfortable on the mice fixing plate 100 and also to reserve sufficient space for the fixing material, the mice 110 and the mice fixing plate 100 are designed as a cuboid with the size of 15cm x 7cm. Thus, 8 such mouse fixing plates 100 can be placed in the area of one loading plate and tissue compensation pad for experiments. The mouse fixing plate 100 is made of foamed plastic, the plate thickness is designed to be 2.5cm, and the thickness can ensure that the mouse fixing plate 100 has enough strength and is not easy to break in use. The foam plastic has the advantages of low cost, moderate elasticity, difficult temperature drastic change along with air temperature, light weight and the like. The mouse fixing plate 100 made of such a material is more comfortable and convenient to transport than wooden and hard plastic plates. This material is less likely to cause accidental injury to the mouse 110 during transport of the mouse 110. The split mouse fixing plate 100 has better flexibility than the integral fixing plate which can accommodate a plurality of mice, and is convenient for more flexible matching with the protective baffle 20.

S6: adhesive fixing

After the mice enter a good anesthetic state, the mice are stuck and fixed. Depending on the tumor inoculation site, either the prone or supine position is selected. The body midline of the mouse is coincident with the central axis 30 of the fixation plate 100 and the tumor is placed in the tumor placement area 101 on the fixation plate. The trunk and tail of the mouse are adhered by an adhesive tape, and are firmly adhered to the fixing plate 100. When the plaster is stuck, the force is not applied, so that the death of the mice caused by mechanical asphyxia is prevented.

Specifically: first, the tumor is matched with the irradiation window 220 of the shielding device, and the tumor placement area 101 is set on the mouse fixing plate 100 in this embodiment. Since the tumor is typically inoculated on only one side of the body of the mouse 110, the tumor placement area 101 is disposed beside the central axis of the mouse fixing plate 100. As previously described, the inoculation sites are different in different experiments, so the same mouse 110, mouse fixation plate 100, needs to meet the placement requirements for tumors at different sites in prone or supine positions. The distance from the nose to the armpit of the mouse was about 3.5cm, the distance from the armpit to the inguinal was about 3.5cm, and the distance from the inguinal to the tail root was 2-2.5cm. The diameter of the subcutaneous tumor in the mice should not exceed 2cm according to the ethical and welfare requirements of the animals, so the tumor placement area 101 is designed as a square area of 2.5cm by 2.5cm. According to the above conditions, one side of the tumor placement area 101 was set on the central axis 30 of the mouse fixing plate 100, and the distance between the center of the placement area and the lower edge of the mouse fixing plate 100 was set to 5cm.

S7: tumor identification

The tape was outlined with an oil marker to align with the irradiation window of the radiation shield. (the tissue compensation pad covering the mouse is translucent and the tumor site cannot be clearly observed without marking).

Specifically: the central axis 30 of the fixing plate is marked as a mark for placing the body of the mouse because the natural central axis formed by the nose and mouth of the mouse, the spine and the tail of the mouse is an obvious anatomical mark. The tumor is placed in the tumor placement area 101, and the natural central axis is overlapped with the central axis 30 of the fixing plate, so that uniform placement of all mice can be realized, and standardization of experimental procedures is facilitated. The alignment reference line 222 is designed on the protective baffle 20 according to the positioning mark of the central axis 30 on the fixed plate, and the two lines are overlapped to realize the matching and alignment of the fixed plate and the protective device.

S8: transportation of

The fixed mice are placed in a box with good ventilation and transported to the radiotherapy room together with the protective shield 20, lead block 230, adhesive tape, anesthetic and anesthetic device.

S9: if necessary, additional anesthesia and fixation

If the mice are awakened, restless and unstable before proceeding to the next step due to some reasons, the mice can be additionally anesthetized through the peritoneal anesthesia hole, and the fixing adhesive tape can be adjusted or added if necessary, so that the mice can smoothly enter the next procedure.

Referring to fig. 4, specifically: to solve the problem of accidental agitation of the mice 110 during anesthesia, additional anesthesia is often required, and the completion of the intraperitoneal injection operation requires separation of the prone position mice 110 from the fixture, which undoubtedly increases the time of the experiment. For prone position mice 110, the present embodiment solves this problem by designing the abdominal anesthesia zone 102 on the mice 110 and the mouse fixation plate 100. In order to fully expose the puncture area, the inner edge of the anesthesia window is arranged at the position 0.8cm on the left side of the central axis of the mouse fixing plate 100, and the anesthesia window is designed to be a square area of 1.5cm by 1.5 cm. The location of the anesthesia window varies among tumors in different locations, so three locations of different peritoneal anesthesia zones 102 are designed.

The fixing device aims to solve the problems of safety, innocuity, reliable fixing, convenient operation and observation, convenient marking and convenient midway additional fixing required by the fixing of the mice 110. The skin of the trunk and the rear legs of the mouse 110 and the mouse fixing plate 100 are firmly adhered together using a 6cm wide and transparent upper fixing tape 111; the rat tail was firmly attached to the mouse fixing plate 100 with the aid of a 3cm wide and transparent lower fixing tape 112.

S10: positioning for radiotherapy

The mice are placed side by side on a supporting plate 10 matched with a radiotherapy bed, and are positioned by a radiotherapeutic apparatus operated by a radiotherapeutic operator. The mice were then covered with tissue compensating pads.

S11: paired with protective baffles

The protective shield 20 is placed on the radiation therapy couch carrier plate and the tumor is exposed within the irradiation window by aligning the protective lead upper shield 210 with the identification lines on the fixation plate 100.

Specifically: the protection shield 20 is designed according to the mouse 110 and the mouse fixing plate 100. One loading board 10 can be provided with 8 mice fixing boards 100 at the same time, in order to increase the flexibility of the radiotherapy protection baffle 20 in use, 2 protection baffles 20 are designed, and each protection board can meet the protection of 4 mice 110 at most. Each protective shield 20 is made of a lead plate 5mm thick, and 4 mouse fixing plates 100 are placed side by side, respectively, at the opposite positions of the tumor placement area 101.

S12: adjusting an illumination window

The area of the irradiation window was adjusted by using the window-adjusting lead block 230 so that the normal tissue of the mouse was exposed to the radiation as little as possible. The position of the lead block 230 is recorded by a scale beside the window to provide a data reference for the next radiotherapy window.

Referring to fig. 5, specifically: on the surface of each lead top baffle 210, 4 square irradiation windows 220 with a side length of 2.5cm are respectively formed, and an alignment reference line 222 is arranged beside each irradiation window 220, so that the tumor placement area 101 is aligned with the irradiation window 220 by using the line. Each irradiation window 220 is attached with 2 lead blocks 230 with the side length of 4cm, and the area of the irradiation window 220 can be flexibly adjusted according to the tumor size by covering the edges of the irradiation windows 220. The position covered by the lead block 230 is recorded by the attachable scale 221 on the edge of the illumination window 220, which allows for standardization of operation. The two ends of the lead plate are welded with the side plates with the inner height of 7cm, and the protective baffle 20 can be conveniently and stably arranged on the bearing plate 10 of the radiotherapy bed by adopting the structure. The space formed under the protective barrier 20 is used to accommodate the mouse 110, the mouse fixing plate 100, the mouse 110, and the radiation therapy cushion 120. This space is slightly larger than the volume of the contents, which is advantageous in that it allows a sufficient space for the researcher to operate and observe during tumor alignment and the like, while not significantly expanding the area of the irradiation field formed by the radiation transmitted through the irradiation window 220.

S13: performing irradiation

Irradiation of the mouse tumor was performed by a radiotherapeutic according to the irradiation dose in the experimental plan.

S14: transportation of

After the end of radiotherapy, the mice were transported back to the laboratory.

S15: dismantling the fixing

After returning to the laboratory, the adhesive tape stuck on the mouse body is quickly removed, and the restraint of the mouse is released.

The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present invention, and are not intended to limit the invention, and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The special fixing and protecting device for the mouse radiotherapy comprises a mouse fixing plate (100) for fixing an experimental mouse (110) and a protecting baffle (20) matched with the mouse fixing plate, wherein the protecting baffle is compatible with a radiotherapy bearing plate (10) and a radiotherapy rubber pad (120) used clinically;

the mouse fixing plate (100) is arranged on the bearing plate (10), a central axis (30) is marked on the upper surface of the mouse fixing plate (100), and a tumor placement area (101) and an abdominal anesthesia area (102) are arranged beside the central axis (30); the positions of the peritoneal anesthesia areas (102) are different in tumors at different positions, and three different peritoneal anesthesia areas (102) are designed;

the two groups of the protective baffles (20) are arranged, each group of the protective baffles (20) is formed by welding an upper baffle (210) and two side baffles (200), and a space formed below the protective baffles (20) is used for accommodating a mouse fixing plate (100), an experimental mouse (110) and a radiotherapy rubber pad (120);

an irradiation window (220) is formed in the surface of the upper baffle plate (210), two lead blocks (230) with the surface length and the surface width of 4cm are placed on the surface of the irradiation window (220), and the area of the irradiation window (220) can be flexibly adjusted according to the tumor size by covering the edges of the irradiation window (220) with the lead blocks (230); an alignment reference line (222) is arranged beside each irradiation window (220), and a sticky graduated scale (221) is arranged on the edge of each irradiation window;

the upper baffle plate (210) and the side baffle plates (200) are both made of lead materials.

2. The fixation guard for radiation therapy of mice as claimed in claim 1, wherein: the mouse fixing plate (100) is a cuboid with the size of 15cm by 7cm, is made of foam plastic, and has the plate thickness of 2.5cm.

3. The fixation guard for radiation therapy of mice as claimed in claim 1, wherein: the experimental mice (110) are fixed on the mice fixing plate (100) through a binding object (40), and the binding object (40) comprises an upper fixing adhesive tape (111) and a lower fixing adhesive tape (112) which are transparent plastic adhesive tapes.

4. The fixation guard dedicated to radiation therapy of mice as claimed in claim 2, wherein: the two groups of four mouse fixing plates (100) are arranged up and down along the length direction of the plates and then are matched with the size of a radiotherapy machine.

5. The fixation guard for radiation therapy of mice as claimed in claim 1, wherein: the radiotherapy rubber pad (120) is 30cm by 1.0cm.

6. The fixation guard for radiation therapy of mice as claimed in claim 1, wherein: the thickness of the upper baffle plate (210) and the side baffle plates (200) is 5mm, and the height of the side baffle plates (200) is 7cm.

7. The fixation guard for radiation therapy of mice as claimed in claim 1, wherein: the surface of each upper baffle plate (210) is provided with 4 square irradiation windows (220) which are equidistant and have a side length of 2.5cm.

8. The method of using a fixation guard dedicated to radiation therapy of mice as claimed in claim 3, wherein: firstly, placing the body of an experimental mouse (110) at the positions corresponding to the tumor placement area (101) and the abdominal anesthesia area (102), then fixing the trunk, the rear legs and the other part of the tail of the experimental mouse (110) on the mouse fixing plate (100) through the upper fixing adhesive tape (111) and the lower fixing adhesive tape (112) respectively, then covering the experimental mouse (110) with the radiotherapy rubber pad (120), placing the protective baffle (20) above the mouse fixing plate (100), the experimental mouse (110) and the radiotherapy rubber pad (120), then adjusting the irradiation window to carry out radiotherapy irradiation, returning the experimental mouse (110) to the original laboratory after the radiotherapy is finished, and finally dismantling and fixing.