CN116650615B - Composition with oral care effect and application thereof - Google Patents

Composition with oral care effect and application thereof Download PDF

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CN116650615B
CN116650615B CN202310934765.1A CN202310934765A CN116650615B CN 116650615 B CN116650615 B CN 116650615B CN 202310934765 A CN202310934765 A CN 202310934765A CN 116650615 B CN116650615 B CN 116650615B
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parts
composition
oral care
dispersed
plant lactobacillus
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CN116650615A (en
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刘爽
刘晶琦
王佳
刘爱青
王海燕
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Beijing Semnl Biotechnology Co ltd
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    • A61K38/01—Hydrolysed proteins; Derivatives thereof
    • A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
    • A61K38/014—Hydrolysed proteins; Derivatives thereof from animals from connective tissue peptides, e.g. gelatin, collagen
    • A—HUMAN NECESSITIES
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    • A61K31/525—Isoalloxazines, e.g. riboflavins, vitamin B2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/00—Medicinal preparations containing organic active ingredients
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    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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    • A61K35/74—Bacteria
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/534—Mentha (mint)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
    • A—HUMAN NECESSITIES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
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    • A61K9/2013—Organic compounds, e.g. phospholipids, fats
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Abstract

The invention belongs to the field of oral care, and particularly relates to a composition with an oral care effect and application thereof. The raw materials of the composition comprise the following components in parts by weight: 80-200 parts of collagen peptide and 5-40 parts of dispersed lactobacillus plantarum. The preparation method comprises mixing and tabletting. The oral care composition provided by the invention has the advantages that collagen peptide and dispersed plant lactobacillus powder can synergistically improve the activity of human periodontal ligament cells, can induce the expression of osteogenic differentiation related genes and proteins, and has good osteoinductive property; meanwhile, the collagen peptide has the effects of promoting wound healing, inhibiting inflammation, relieving allergy, adhering, antagonizing adhesion and the like, and has good synergistic effect on repairing the oral ulcer.

Description

Composition with oral care effect and application thereof
Technical Field
The invention belongs to the field of oral care, and particularly relates to a composition with an oral care effect and application thereof.
Background
Seven significant oral problems are noted, including caries, gum problems, tooth stains, plaque, calculus, tooth sensitivity, and breath. In recent years, the strengthening of the "skin care" of the oral cavity is broken through by the boundary components, the food components are taken into the oral racetrack, such as the probiotic mouthwash and the hyaluronic acid toothpaste, but other edible oral products with health care and restoration are rarely available in the market besides chewing gum for freshening breath.
Chinese patent application 201610236550.2 discloses a Chinese herbal toothpaste containing fish collagen peptide, which comprises
The toothpaste comprises, by weight, 3-8 parts of fish collagen peptide, 1-6 parts of radix scutellariae extract, 1-6 parts of sarcandra glabra extract, 3-8 parts of pseudo-ginseng root powder and 72-92 parts of toothpaste matrix. The extracts are used together, and the combined action of fish collagen can be used for treating stomatitis, protecting oral cavity inner membranes, reducing oral diseases, refreshing and benefiting mouths.
Another chinese patent application No. 201610662975.X discloses oral care compositions containing probiotics and their use in toothpastes. The oral care composition is prepared from lactobacillus, polysaccharide and span according to the following steps: (1) dissolving span in water at 30-40 ℃; (2) Adding lactobacillus, stirring for 15-20 minutes to obtain a mixed solution, and freeze-drying at-10 ℃ to-30 ℃; (3) Mixing oligosaccharide with the obtained lyophilized powder uniformly. The oral care product added with the oral care composition containing probiotics has the effects of inhibiting dental plaque generation, resisting gingivitis, removing halitosis and maintaining oral health.
Still another chinese patent application 201811244925.5 discloses a biological composition for preventing and treating oral cancer, which is prepared from the following raw materials in parts by weight: 1-3 parts of haw extract, 6-9 parts of corn peptide, 9-11 parts of rhizoma polygonati extract, 2-5 parts of lactobacillus acidophilus, 8-11 parts of pachyman, 2-5 parts of chicken collagen, 7-10 parts of costustoot, 5-8 parts of bifidobacterium, 10-13 parts of phyllanthus emblica extract, 11-15 parts of eggplant extract, 1-3 parts of rimonabant, 2-5 parts of collagen polypeptide, 6-9 parts of lactobacillus, 5-8 parts of rhizoma acori graminei, 1-3 parts of borneol, 7-10 parts of haw, 3-7 parts of clostridium butyricum, 3-7 parts of sea buckthorn, 8-10 parts of macleaya cordata extract and 1-3 parts of phellodendron bark extract. The invention uses animal and plant extract powder which is strictly screened, strains and ionized water are inoculated for anaerobic fermentation. After fermentation, the activity of the beneficial bacteria is ensured, the drug effect is improved, the drug property is changed, and the drug effect is durable and stable.
The prior art has conducted a certain research on oral care products, but the oral care products have the characteristics that the components are more in general, the raw materials are non-food raw materials, and the oral care effect is not obvious enough.
Disclosure of Invention
In response to the above-mentioned shortcomings, the present invention provides a composition having oral care efficacy.
In order to solve the technical problems, the invention adopts the following technical scheme:
a composition with oral care efficacy, the raw materials of the composition comprise the following components in parts by weight: 80-200 parts of collagen peptide and 5-40 parts of dispersed lactobacillus plantarum.
Preferably, the raw materials of the composition further comprise one or more of aloe vera gel, peppermint, sodium hyaluronate, vitamin B2 and vitamin C.
Preferably, the raw materials of the composition further comprise sodium hyaluronate, vitamin B2, vitamin C, aloe vera gel and/or peppermint.
Preferably, the raw materials of the composition further comprise the following components in parts by weight: 0.5-1.5 parts of sodium hyaluronate, 0.1-1.0 parts of vitamin B, 40-60 parts of vitamin C, 25-50 parts of aloe vera gel and/or 25-50 parts of peppermint.
Collagen is rich in glycine, proline, hydroxyproline and other amino acids required by human body, is an important component in extracellular matrix, is also a protein with the most abundant content in human body, and accounts for one third of the total protein of human body, namely equivalent to six percent of the weight of human body, and is distributed throughout various organs of human body, such as: skin, bone, cartilage, ligament, cornea, various intima and fascia, etc., are main components for maintaining the morphology and structure of skin and tissue organs, and are important raw material substances for repairing various damaged tissues.
The collagen peptide is formed by hydrolyzing macromolecular collagen in animal bodies, such as animal skin, bones and the like, is easily seen in the market at present, and has the characteristics of small molecular weight and easy absorption by human bodies, and is widely applied to the oral beauty skin care market. However, as collagen peptides have been studied more and more recently, the scope of the studies has been increased, and the application direction has been diversified, such as bone joints, hair, pet foods, etc. In recent years, the efficacy of collagen peptides in oral care has also begun to be gradually demonstrated.
Human periodontal ligament cells (hPDLC) are functional cells in the periodontal membrane and are considered ideal cell types for reconstruction of alveolar bone, cementum and periodontal membrane. hPLCC can differentiate into fibroblasts, osteoblasts, etc., and form mineralized tissues, and plays a key role in periodontal repair and regeneration. The research shows that collagen peptide can raise the activity of human periodontal membrane cell and induce the expression of osteogenic differentiation related gene and protein.
Meanwhile, collagen peptide has a certain therapeutic effect on oral ulcer, and researches show that the tissue of the oral ulcer part treated by the collagen peptide group is basically healed, epithelial cells are closely arranged, denatured cells are occasionally seen among the epithelial cells, and inflammatory cells are not seen; pathological observation shows that the tissue injury of the canker sore part treated by the collagen peptide group is light, the necrosis degree is light, and granulation tissue and fibrosis are obvious.
The aloe vera gel is prepared by taking fresh aloe vera leaves as raw materials and adopting a series of technologies of peeling, refining, separating, stabilizing, purifying and the like, is free from adding essence, alcohol and pigment, retains various active nutritional ingredients in aloe, and has various effects of moisturizing, nourishing and moistening, resisting sensitization and repairing, balancing grease secretion, preventing spots and acnes, delaying aging, nourishing hair and the like.
Menthol contained in peppermint can excite central nerve, dilate skin capillary vessel, promote sweat gland secretion, has effects of inducing sweat, relieving fever, diminishing inflammation and relieving pain, can relieve itching, can be used for treating common cold due to wind heat, sore throat, skin itch, etc., and can also play a role in refreshing oral cavity after peppermint enters oral cavity.
Hyaluronic acid is a major component constituting the matrix of the cytoplasm and extracellular space, and is widely distributed in human tissues, with the highest content in dermis layers of human skin and joint synovial fluid. Hyaluronic acid has been widely used in the fields of medicine, cosmetics, functional foods, etc. because of its excellent moisture-retaining property, viscoelasticity and biocompatibility. In recent years, with the intensive research of hyaluronic acid foundation and the accumulation of clinical experience, the characteristics of anti-inflammatory property, antibacterial property, osteogenesis, anti-edema, healing promotion and the like of hyaluronic acid are continuously discovered and applied to the treatment of oral diseases, and various domestic and foreign researches show that hyaluronic acid has good auxiliary effects on the treatment of periodontal diseases, oral mucosa diseases and dental pulp diseases, and in the aspect of oral care, hyaluronic acid can moisten the oral cavity, improve dry mouth, inhibit dental plaque, improve gum health conditions and the like.
Vitamin B2, also known as riboflavin, is one of the B vitamins, slightly soluble in water, and stable upon heating in neutral or acidic solutions. Is an integral part of the Huang Meilei prosthetic group in vivo, which promotes development and regeneration of cells; promoting normal growth of skin, nails, hair; helps prevent and eliminate inflammatory reactions in the mouth, lips, tongue and skin, collectively referred to as the stomatogenital syndrome; improving vision and relieving eye fatigue; influence the iron absorption of human body; in combination with other substances, thereby affecting biological oxidation and energy metabolism.
Vitamin C is a highly potent antioxidant used to relieve the oxidative stress of the ascorbate peroxidase substrate. Vitamin C is also required to participate in many important biosynthesis processes, such as promotion of collagen synthesis, and thus to facilitate nutritional repair of bone joints. Can also promote antibody formation, enhance immunity, and promote absorption of calcium and iron.
Preferably, the raw materials of the composition further comprise a lubricant, sweetener and/or flavoring agent.
Preferably, the lubricant is magnesium stearate, the sweetener is one or more of erythritol, fructo-oligosaccharide, xylitol, stevioside, fructus momordicae extract and sucralose, and the flavoring agent is one or more of sweet orange powder, rose powder, peppermint essence, plum powder, yoghurt powder and green tea essence.
Preferably, the composition is in the form of a tablet, capsule, granule, powder, jelly or solution.
Preferably, the composition is a tablet, and the preparation method comprises mixing the raw materials for 5-15min, tabletting with a tabletting machine at a revolving speed of 25-30rpm, and making into tablet.
It is a further object of the present invention to provide the use of the above composition in the preparation of a product for oral care.
Preferably, the oral care is to repair dental ulcers or inhibit oral inflammation.
Compared with the prior art, the invention has the following positive and beneficial effects:
(1) The oral care composition provided by the invention has the advantages that collagen peptide and dispersed plant lactobacillus powder can synergistically improve the activity of human periodontal ligament cells, can induce the expression of osteogenic differentiation related genes and proteins, and has good osteoinductive property; meanwhile, the collagen peptide has the effects of promoting wound healing, inhibiting inflammation, relieving allergy, adhering, antagonizing adhesion and the like, and has good synergistic effect on repairing the oral ulcer.
(2) The common plant lactobacillus is easy to coagulate and agglomerate, and is difficult to permeate Peer-collecting lymph nodes (M cells) of intestinal mucosa after coagulation, so that human bodies are difficult to absorb, and immune response is reduced.
Detailed Description
The present invention will be described in further detail with reference to the following examples, which are not intended to limit the present invention, but are merely illustrative of the present invention. The experimental methods used in the following examples are not specifically described, but the experimental methods in which specific conditions are not specified in the examples are generally carried out under conventional conditions, and the materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.
Wherein, the supplier of the dispersed lactobacillus plantarum is Beijing Cheng Meinuo biotechnology Co., ltd, the commodity name is the dispersed lactobacillus plantarum powder SNK, and the dispersed lactobacillus plantarum powder is the inactivated bacteria powder produced by the processes of culturing, sterilizing, spray drying and the like of lactobacillus plantarum SNK12 strain.
The starting materials for examples 1-8 are shown in Table 1 below.
TABLE 1
The preparation methods of examples 1-8 are as follows.
Example 1
(1) Mixing the ingredients: xylitol (Shandong Bailong Crohn Biotech Co., ltd.), collagen peptide (Beijing Cheng Meinuo Biotechnology Co., ltd., supplier: tilapia source, average molecular weight about 500 Da), dispersed plant lactobacillus powder SNK (Beijing Cheng Meinuo Biotechnology Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), magnesium stearate (Henan Jian Jiu Co., ltd.) are placed in a three-dimensional mixer, and mixed and stirred for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is adjusted to 25 revolutions per minute, the pressure of a pressing wheel is adjusted to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
Example 2
(1) Mixing the ingredients: xylitol (Shandong Bailong Crohn Biotechnology Co., ltd.), collagen peptide (tilapia source, average molecular weight about 500 Da, beijing Cheng Meinuo Biotechnology Co., ltd.), dispersed plant lactobacillus powder SNK (Beijing Cheng Meinuo Biotechnology Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and 3 mg/tablet of magnesium stearate (Henan Jian Jiu Co., ltd.) are placed in a three-dimensional mixer, and mixed and stirred for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is adjusted to 25 revolutions per minute, the pressure of a pressing wheel is adjusted to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
Example 3
(1) Mixing the ingredients: weighing raw materials and auxiliary materials according to a formula ratio, placing xylitol (Shandong Bailong Crohn biotechnology Co., ltd.), collagen peptide (tilapia source, average molecular weight about 500 Da, beijing Cheng Meinuo biotechnology Co., ltd.), dispersed plant lactobacillus powder SNK (Beijing Cheng Meinuo biotechnology Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.) and magnesium stearate (Henan Jian Jiu Co., ltd.) into a three-dimensional stirrer, and mixing and stirring for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is adjusted to 25 revolutions per minute, the pressure of a pressing wheel is adjusted to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
Example 4
(1) Mixing the ingredients: xylitol (Shandong Bailong Innovative Biotech Co., ltd.), collagen peptide (tilapia source, average molecular weight about 500 Da, supplier Beijing Cheng Meinuo Biotechnology Co., ltd.), dispersed plant lactic acid bacteria powder SNK (Beijing Cheng Meinuo Biotechnology Co., ltd.), aloe vera gel powder (Yunwan Green Biotechnology Co., ltd.), sodium hyaluronate (Hua Xi Biotechnology Co., ltd.), vitamin C (stone medicine group, food grade), vitamin B2 (Henan Hill chemical Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), magnesium stearate (Henan Jian Jiu Co., ltd.) are placed in a three-dimensional mixer, and mixed and stirred for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is adjusted to 25 revolutions per minute, the pressure of a pressing wheel is adjusted to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
Example 5
(1) Mixing the ingredients: weighing raw materials and auxiliary materials according to a formula proportion, namely xylitol (Shandong Bailong Crohn Biotechnology Co., ltd.), collagen peptide (tilapia source, average molecular weight about 500 Da, supplier Beijing Cheng Meinuo Biotechnology Co., ltd.), dispersed plant lactobacillus powder SNK (Beijing Cheng Meinuo Biotechnology Co., ltd.), peppermint powder (Zhengzhou Huafeng food technology Co., ltd.), sodium hyaluronate (Hua Xi Biotechnology Co., ltd.), vitamin C (stone medicine group, food grade), vitamin B2 (Henan Highway chemical Co., ltd.), microcrystalline cellulose (Henan Jian Co., ltd.), magnesium stearate (Henan Jian Co., ltd.), placing the mixture in a three-dimensional stirrer, and mixing and stirring for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is adjusted to 25 revolutions per minute, the pressure of a pressing wheel is adjusted to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
Example 6
(1) Mixing the ingredients: weighing raw materials and auxiliary materials according to a formula proportion, wherein xylitol (Shandong Bailong Crypton Biotechnology Co., ltd.), collagen peptide (tilapia source, average molecular weight about 500 Da, supplier Beijing Cheng Meinuo Biotechnology Co., ltd.), dispersed plant lactobacillus powder SNK (Beijing Cheng Meinuo Biotechnology Co., ltd.), aloe vera gel powder (Yunnan Green Biotechnology Co., ltd.), peppermint powder (Zhengzhou Huafeng food technology Co., ltd.), sodium hyaluronate (Hua Xi Biotechnology Co., ltd.), vitamin C (stone medicine group, food grade), vitamin B2 (Henan Highway chemical Co., ltd.), microcrystalline cellulose (Henan Jian Hill Co., ltd.), magnesium stearate (Henan Jian Co., ltd.), placing into a three-dimensional mixer, and mixing and stirring for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is adjusted to 25 revolutions per minute, the pressure of a pressing wheel is adjusted to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
Example 7
(1) Mixing the ingredients: weighing raw materials and auxiliary materials according to a formula proportion, namely xylitol (Shandong Bailong Crypton Biotechnology Co., ltd.), collagen peptide (tilapia source, average molecular weight about 500 Da, supplier Beijing Cheng Meinuo Biotechnology Co., ltd.), dispersed plant lactobacillus powder SNK (Beijing Cheng Meinuo Biotechnology Co., ltd.), aloe vera gel powder (Yunwan Green biological Co., ltd.), peppermint powder (Zhengzhou Huafeng food technology Co., ltd.), sodium hyaluronate (Hua Xi Biotechnology Co., ltd.), vitamin C (stone medicine group, food grade), vitamin B2 (Henan Highway chemical Co., ltd.), microcrystalline cellulose (Henan Jian Hill Co., ltd.), magnesium stearate (Henan Hill Co., ltd.), green tea essence (Hangzhou Pasteur essence perfume Co., ltd.), and placing the mixture in a three-dimensional mixer, and mixing for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is regulated to 30 revolutions per minute, the pressure of a pressing wheel is regulated to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
Example 8
(1) Mixing the ingredients: raw materials and auxiliary materials are weighed according to a formula proportion, xylitol (Shandong Bailong Crohn biotechnology Co., ltd.), collagen peptide (tilapia source, average molecular weight about 500 Da, supplier is Beijing Cheng Meinuo biotechnology Co., ltd.), dispersed plant lactobacillus powder SNK (Beijing Cheng Meinuo biotechnology Co., ltd.), aloe vera gel powder (Yunnan Wan Green biotechnology Co., ltd.), peppermint powder 25 mg/tablet (Zhengzhou Huafeng food technology Co., ltd.), sodium hyaluronate (Hua Xi biotechnology Co., ltd.), vitamin C (stone medicine group, food grade), vitamin B2 (Henan Heshihe chemical Co., ltd.), microcrystalline cellulose (Henan Dai Jiu Co., ltd.), magnesium stearate (Henan Dai Jiu Co., ltd.), peppermint essence (Hangzhou Bai Rumex perfume Co., ltd.) are placed in a three-dimensional stirring machine, and mixed and stirred for about 10 minutes;
(2) And (3) discharging and tabletting: when in tabletting and forming, the temperature and the ambient humidity should be controlled, the relative humidity is below 60 percent, the weight of the filling wheel to a tablet is 500mg, the rotating speed of a turntable of a tablet press is adjusted to 25 revolutions per minute, the pressure of a pressing wheel is adjusted to ensure that the hardness of the tablet reaches the requirement, and the phenomena of sticking, punching and the like are avoided.
The formulation materials of comparative examples 1 to 7 are shown in Table 2 below.
TABLE 2
Comparative examples 1-7 were prepared as follows.
Comparative example 1
Mixing collagen peptide (tilapia source, average molecular weight about 500 Da, supplier is Beijing Cheng Meinuo Biotechnology Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and magnesium stearate (Henan Jian Jiu Co., ltd.) at a certain ratio; then, the procedure was followed as in step (2) of example 1.
Comparative example 2
Mixing collagen peptide (tilapia source, average molecular weight about 500 Da, supplier is Beijing Cheng Meinuo Biotechnology Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and magnesium stearate (Henan Jian Jiu Co., ltd.) at a certain ratio; then, the procedure was followed as in step (2) of the above-mentioned example 1.
Comparative example 3
Mixing collagen peptide (tilapia source, average molecular weight about 500 Da, supplier is Beijing Cheng Meinuo Biotechnology Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and magnesium stearate (Henan Jian Jiu Co., ltd.) at a certain ratio; then, the procedure was followed as in step (2) of example 1.
Comparative example 4
Mixing with dispersed lactobacillus plantarum powder SNK (Beijing Cheng Meinuo biotechnology Co., ltd.), xylitol (Shandong Bailong Critical Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and magnesium stearate (Henan Jian Jiu Co., ltd.); then, the procedure was followed as in step (2) of example 1.
Comparative example 5
Mixing with dispersed lactobacillus plantarum powder SNK (Beijing Cheng Meinuo biotechnology Co., ltd.), xylitol (Shandong Bailong Critical Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and magnesium stearate (Henan Jian Jiu Co., ltd.); then, the procedure was followed as in step (2) of example 1.
Comparative example 6
Mixing with dispersed lactobacillus plantarum powder SNK (Beijing Cheng Meinuo biotechnology Co., ltd.), xylitol (Shandong Bailong Critical Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and magnesium stearate (Henan Jian Jiu Co., ltd.); then, the procedure was followed as in step (2) of example 1.
Comparative example 7
Mixing with dispersed lactobacillus plantarum powder SNK (Beijing Cheng Meinuo biotechnology Co., ltd.), xylitol (Shandong Bailong Critical Co., ltd.), microcrystalline cellulose (Henan Jian Jiu Co., ltd.), and magnesium stearate (Henan Jian Jiu Co., ltd.); then, the procedure was followed as in step (2) of example 1.
Effect test
The pressed tablets prepared in examples 1 to 8 and comparative examples 1 to 7 were ground into powder, each powder was dissolved in DMEM medium to prepare 1 mg/mL solution, and the solution was stored at-20 ℃ for later use, and divided into 15 groups, with the addition of a blank control group and a model group, and 17 groups in total, and a model was established by stimulating human periodontal ligament fibroblasts (hPDLFs) with Lipopolysaccharide (LPS) as a causative agent, and then the content of IL-6, IL-8 and TNF- α in the supernatant of inflammatory cell model was examined for the different examples and comparative examples. The blank group was cultured in normal DMEM medium, the model group was cultured in DMEM medium containing 10. Mu.g/mL of LPS, and each of the examples and comparative examples was cultured in DMEM medium containing 10. Mu.g/L of LPS and diluted to 300. Mu.g/mL. Finally, IL-6, IL-8 and TNF-alpha are detected according to the instruction of ELISA kit, and the secretion amount of IL-6, IL-8 and TNF-alpha in each milligram of protein is calculated. The experimental results are relative fold differences between the model group, the example, the comparative example and the blank control group as shown in table 3 below.
TABLE 3 Table 3
The significant increase in IL-8 and TNF- α in the model group compared to the placebo group, and the significant decrease in IL-6, indicated that LPS significantly up-regulates the secretion of IL-8 and TNF- α in hPDFs and decreases the secretion of IL-6.
From the data, the IL-8 and TNF- α levels in the groups of examples 6, 7 and 8 were most significantly reduced compared to the model group, and the IL-6 levels were most significantly increased compared to the model group, indicating that these groups reduced the pro-inflammatory effects of LPS over the other examples and the comparative group.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims (9)

1. A composition with oral care effect, which is characterized by comprising the following raw materials in parts by weight: 83.5 parts of collagen peptide, 5 parts of dispersed plant lactobacillus, 1 part of sodium hyaluronate, 0.5 part of vitamin B, 50 parts of vitamin C and 50 parts of aloe vera gel; the dispersed type plant lactobacillus is the dispersed type plant lactobacillus SNK12.
2. A composition with oral care effect, which is characterized by comprising the following raw materials in parts by weight: 83.5 parts of collagen peptide, 5 parts of dispersed plant lactobacillus, 0.5-1.5 parts of sodium hyaluronate, 0.5 part of vitamin B2, 50 parts of vitamin C and 50 parts of mint; the dispersed type plant lactobacillus is the dispersed type plant lactobacillus SNK12.
3. A composition with oral care effect, which is characterized by comprising the following raw materials in parts by weight: 83.5 parts of collagen peptide, 5 parts of dispersed plant lactobacillus, 0.5-1.5 parts of sodium hyaluronate, 0.5 part of vitamin B2, 50 parts of vitamin C, 25 parts of aloe vera gel and 25 parts of peppermint; the dispersed type plant lactobacillus is the dispersed type plant lactobacillus SNK12.
4. A composition according to any one of claims 1 to 3, wherein the raw materials of the composition further comprise a lubricant, sweetener and/or flavouring.
5. The composition of claim 4, wherein the lubricant is magnesium stearate, the sweetener is one or more of erythritol, fructo-oligosaccharides, xylitol, steviol glycosides, fructus momordicae extract and sucralose, and the flavoring agent is one or more of orange powder, rose powder, peppermint essence, plum powder, sour milk powder and green tea essence.
6. The composition with oral care efficacy according to any one of claims 1 to 5, characterized in that the formulation of the composition is a tablet, capsule, granule, powder or solution.
7. The composition of claim 6, wherein the composition is in the form of a tablet and the process comprises mixing the materials for 5-15 minutes and tabletting the mixture with a tabletting machine at a turntable speed of 25-30rpm to obtain a compressed tablet.
8. Use of a composition according to any one of claims 1 to 6 in the manufacture of a product for oral care.
9. The use according to claim 8, wherein the oral care is repairing dental ulcers or inhibiting oral inflammation.
CN202310934765.1A 2023-07-28 2023-07-28 Composition with oral care effect and application thereof Active CN116650615B (en)

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