CN116649358A - Insecticidal composition containing bistrifluron and application thereof - Google Patents

Insecticidal composition containing bistrifluron and application thereof Download PDF

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Publication number
CN116649358A
CN116649358A CN202310635575.XA CN202310635575A CN116649358A CN 116649358 A CN116649358 A CN 116649358A CN 202310635575 A CN202310635575 A CN 202310635575A CN 116649358 A CN116649358 A CN 116649358A
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bistrifluron
percent
active ingredient
insecticidal composition
test
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葛尧伦
张芳
葛晓甜
刘桂娟
刘金玲
张永芳
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Qingdao Hailier Biotechnology Co ltd
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Qingdao Hailier Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the field of pesticide, and in particular relates to a pesticide composition containing bistrifluron and application thereof, wherein the pesticide composition comprises an active ingredient A and an active ingredient B, the active ingredient A is bistrifluron, the active ingredient B is any one of dinotefuran, spinosad, flonicamid and nitenpyram, and the mass ratio of the active ingredient A to the active ingredient B is 1:30-25:1. The insecticidal composition has the advantages of synergism, resistance reduction and the like, and can be used for controlling agricultural, forestry and gardening pests.

Description

Insecticidal composition containing bistrifluron and application thereof
The invention relates to a insecticidal composition containing bistrifluron and application thereof, which are classified as 202111303461.2, 2021, 11, 5 and the name of the insecticidal composition.
Technical Field
The invention belongs to the field of pesticides, and particularly relates to an insecticidal composition containing bistrifluron and application thereof.
Background
Bistrifluoracene is a high-efficiency insect growth regulator screened from benzoyl urea derivatives, and has the chemical name: 1- [ (2-fluoro-3, 5-bis (trifluoromethyl) phenyl) -3- (2, 6-difluorobenzoyl) ] urea. The benzoyl urea derivatives (BPUS) are Insect Growth Regulators (IGRs) which inhibit the synthesis of insect chitin and have stomach toxicity, have higher specificity, are very effective on young insects, can be rapidly degraded in the environment, and have low toxicity on mammals. The chemical structural formula is as follows:
Spinosad (spinosad) is a secondary metabolite produced by fermentation of actinomycetes spinosad (Saccharopolyspora spinosa), and is a biological pesticide directly extracted from fermentation broth. Nicotinic acetylcholine receptor (nAChR) allosteric modulators-binding site I-affect insect neural activity. Allosteric activation of its receptor (unlike binding site-II of group 32) causes the insect nervous system to be hyperexcitable. In addition to main components a and D, there is B, C, E, F, H, J. The first generation of spinosad pesticides is mainly used for controlling lepidoptera and thysanoptera pests.
Dinotefuran (dinotefuran) is a competitive modulator of nicotinic acetylcholine receptors (nachrs) acting on the insect nervous system. Binding to the acetylcholine sites on its receptors causes a range of symptoms in insects from hyperexcitability to somnolence and paralysis. Acetylcholine is the major excitatory neurotransmitter in the insect central nervous system. Dinotefuran is a third generation of nicotinic insecticide, can be quickly absorbed by plants and is widely distributed in the plants.
Flonicamid (floxamid) is a novel low-toxicity picolinamid insect growth regulating pesticide discovered and developed by Japanese stone original company, has the effects of contact killing, internal absorption and permeation, has good neurotoxicity and antifeedant activity for sucking mouthparts pests such as aphids and the like, and the antifeedant activity is irrecoverable.
Nitenpyram (nitenpyram) is a class of neonicotinoid insecticides that act primarily on the insect nervous system and have a neuromodulation effect on the synaptic receptors of pests. Nitenpyram has good systemic and osmotic effects, can be absorbed and transmitted by plant tissues, and has the characteristics of high efficiency, low toxicity, long lasting period and the like for piercing-sucking mouthpart pests.
In the actual process of agricultural production, the generation of pest drug resistance has become an increasingly urgent problem, and the establishment of proper drug resistance treatment measures is an important component in pest comprehensive control. The development of a new medicament has longer time, more investment and faster elimination speed of the medicament due to the pest resistance problem. Therefore, the optimal pesticide without cross resistance is selected for compounding, and the generation of effective pest resistance prevention is a key in agricultural pest prevention. Through experimental researches of the inventor, the compound of the bistrifluron, spinosad, dinotefuran, flonicamid and nitenpyram can generate good synergistic effect on pests, reduce the drug resistance of the pests, reduce the dosage of the medicament, reduce the residual quantity of the pesticide on crops and reduce the environmental pollution.
Disclosure of Invention
The invention aims to provide the insecticidal composition containing the bistrifluron, which has the advantages of synergy, low use cost and good control effect.
In order to achieve the aim, the invention discloses an insecticidal composition containing bistrifluron and application thereof, the insecticidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is bistrifluron, the active ingredient B is any one of dinotefuran, spinosad, flonicamid and nitenpyram, and the mass ratio of the active ingredient A to the active ingredient B is 1:30-25:1.
Further, the mass ratio of the bistrifluron to the spinosad is 1:10-25:1;
further, the mass ratio of the bistrifluron to the spinosad is 1:10-6:1;
further, the mass ratio of the bistrifluron to the spinosad is 1:10, 1:8, 1:3, 2:5, 3:1, 6:1
Further, the mass ratio of the bistrifluron to the dinotefuran is 1:15-10:1;
further, the mass ratio of the bistrifluron to the dinotefuran is 2:9-10:1;
further, the mass ratio of the bistrifluron to the dinotefuran is 2:9, 3:4, 5:1 and 10:1;
further, the mass ratio of the bistrifluron to the flonicamid is 1:25-10:1;
Further, the mass ratio of the bistrifluron to the flonicamid is 2:15-7:1;
further, the mass ratio of the bistrifluron to the flonicamid is 2:15, 1:8, 3:2, 1:3 and 7:1;
further, the mass ratio of the bistrifluron to the nitenpyram is 1:10-7:1;
further, the mass ratio of the bistrifluron to the nitenpyram is 1:7-7:1;
further, the mass ratio of the bistrifluron to the nitenpyram is 1:7, 1:3, 2:5, 5:1 and 7:1;
further, the content of the active ingredient A and the active ingredient B in the insecticidal composition is 1 to 80wt%, preferably 2 to 60wt%;
further, the insecticidal composition comprises an auxiliary agent in addition to the active ingredient, wherein the auxiliary agent is selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, stabilizing agents, penetrating agents and carriers;
further, the wetting agent is selected from one or a mixture of more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrating agent F, chinese honeylocust fruit powder, silkworm excrement or soapberry powder;
further, the dispersing agent is selected from one or more of polycarboxylate, lignosulfonate, alkylphenol ethoxylate formaldehyde condensate sulfate, calcium alkylbenzenesulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, alkylphenol ethoxylate, fatty amine ethoxylate, fatty acid ethoxylate or glycerin fatty acid ester ethoxylate;
Further, the emulsifier is selected from one or a mixture of more of calcium alkylbenzenesulfonate, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), phenylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether disulfonate magnesium salt, triethanolamine salt, benzyl dimethyl phenol polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether or fatty alcohol polyoxyethylene ether;
further, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;
further, the disintegrating agent is selected from one or a mixture of more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;
Further, the antifreezing agent is selected from one or a mixture of more of ethylene glycol, propylene glycol, glycerol or urea;
further, the defoamer is selected from one or a mixture of more of silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds or C8-C10 fatty alcohol compounds;
further, the solvent is selected from one or more of N, N-dimethylformamide, cyclohexanone, butyl ether, dimethylbenzene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octyl pyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate or acetonitrile;
further, the stabilizer is selected from one or a mixture of more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate;
further, the penetrating agent is selected from one or a mixture of more of penetrating agent JFC, penetrating agent T, azone or organic silicon;
further, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;
Further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;
all of the above materials are commercially available.
The insecticidal composition can be prepared into any one of agriculturally acceptable preparation dosage forms according to the requirements, wherein the preparation dosage forms are selected from any one of powder, granules, soluble powder, soluble granules, soluble tablets, water dispersible granules, wettable powder, water dispersible tablets, dispersible liquid, emulsifiable concentrates, aqueous emulsion, microemulsion, suspending agents, suspoemulsion and soluble liquid;
further, the preparation formulation is selected from suspending agents, emulsifiable concentrates, water dispersible granules and dispersible oil suspending agents;
further, the preparation method of the suspending agent provided by the invention comprises the following steps: sequentially placing active ingredients, a surfactant and other functional additives in a reaction kettle according to the formula proportion, adding water, uniformly mixing, shearing at a high speed, performing wet sanding, and finally homogenizing and filtering to obtain a suspending agent product;
the preparation method of the emulsifiable concentrate comprises the following steps: adding the metered active ingredients, the solvent and the cosolvent into a blending kettle according to the formula proportion, stirring to dissolve the active ingredients, the solvent and the cosolvent, adding the emulsifier, supplementing the rest with the residual solvent, stirring uniformly in the stirring kettle, and filtering to obtain the emulsifiable concentrate;
The preparation method of the water dispersible granule comprises the following steps: adding active ingredients into a carrier according to the formula proportion, adding a surfactant and other functional additives into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to obtain a water dispersible granule product; or spraying water, granulating, drying, and sieving to obtain product;
the preparation method of the dispersible oil suspending agent comprises the following steps: and (3) sequentially placing the active ingredients, the surfactant and other functional additives in a reaction kettle according to the formula proportion, adding oil, uniformly mixing, shearing at a high speed, sanding by a wet method, and finally homogenizing and filtering to obtain the dispersible oil suspending agent product.
An application of the insecticidal composition containing bistrifluron in preventing and controlling agricultural, forestry and gardening pests.
Further, the pests are lepidoptera and hemiptera pests;
further, the Lepidoptera (Lepidoptera) pests include: pseudophaga species (adonites spp.), strongylodes (adonites orana), geotrichum species (Agrotis spp.) (rootworm), geotrichum candidum (Agrotis ipsilon) (black rootworm), cotton leaf looper (Alabama argillacea) (cotton leaf worm), amoeba curena, amyelosis transitella (navel orange borer), anacamptodes defectaria, peach stripe moth (Anarsia lineatella) (peach branch moth), jute spodoptera (Anomis sabulifera) (jute looper), dak looper (Anticarsiag emmatalis), fruit tree looper (Archips argyrospila) (fruittree leafroller), rose looper (rose roller), rose roller an armyworm species (Argyrotaenia spp.) (tortricid moths), a orange strongylon (Argyrotaenia citrana) (orange tortria), an autophaga gamma (Bonagota cranaodes), a indica butterfly (boro cinnara) (rice leaf folder), bucculatrix thurberiella (cotton leaf perforator), a leptosphaera species (calypslightly spp.) (leaf miners), a capra reinocula, a peach moth (Carposina niponensis) (peach frui moths), a graminia species (Chilo.), a mango cross-hair moth (Chlumetia transversa) (mango shoot borer), a rose color armyworm (Choristoneura rosaceana) (rose leaf roller (obliquebanded leafroller)), a noctuid species (chrysodenixis spp.), a spp.) Rice leaf roll She Yeming (Cnaphalocerus medinalis) (grass leaf roller), bean flour butterfly species (Colias spp.), lychee line moth (Conpomorpha cramerella), aromatic wood moth (carpenter moth), grass borer species (Crambus spp.), meadow moth (Sod webshank), plum fruit borer (cydiafimbria) (Mei Guoe (plus fruit mole)), pear fruit borer (Cydia mole) and Cydia nilla (oriental fruit moth), cydia nita (pea mole), apple fruit moth (codling moth), darna di dus the species of the genus silk borer (Diaphania spp.) (stem borers), the species of the genus borer (diapraea spp.) (walk borers), the species of the species sugarcane borer (Diatraea saccharalis) (sugarcane borer), the species of the species southwest corn stalk borer (Diatraea graniosella) (southwest corn borer (southwester corn borer)), the species of the genus diamond (earia spp.) (cotton bollworm), the species of the species Eartan diamond (earia insolata) (Egyptian cotton bollworm), the species emerald diamond borer (earia virella) (rough northern bollworm), ecdytopopha aurantianum, the species of the species southern corn seedling borer (Elasmopalpus lignosellus) (small corn stem borer (lesser cornstalk borer)), epiphysias postruttana (apple brown moth (light brown apple moth)), the species of the species leaf borer (Ephestifia spp.) (flow moths), the plant species may be selected from the group consisting of leaf rollers (Ephestia cautella) (almond mole), tobacco leaf rollers (Ephestia elutella) (tobacco moth (tobbaco mole)), mediterranean leaf rollers (Ephestia kuehniella) (mediterranean moth (Mediterranean flour moth)), episeecs species (ephemerides) and nocturnal moth (ephemeria apoma), banana butterfly (Erionota thiax) (banana paper), ligustrum japonicum (Eupoecilia ambiguella) (grape leaf rollers (gram berry mole)), eurocera auxialis (primordica), feltia species (Feltia spp) (cutting root), cerclara species (Gortrapa spp) (stem borers), oriental moth (Grapholita molesta), three-leaf rollers (Hedylepta indicate) (bean leaf rollers (ben) and fruit rollers (leaf rollers), fruit rollers (24), fruit rollers (leaf rollers) and fruit rollers (24), fruit rollers (leaf rollers) and helicopters (budworms), fruit rollers (helicopters) (5, helicopters) and (helicopters) (24), helicopters (helicopters), helicopters (24), helicopters (helicopters) and (45, helicopters) and (helicopters) of the genus (helicopters) (Leucinodes orbonalis) The species of the genus Loxagrotis (Lobelia botana), loxagrotis spp, loxagrotis albicosta (western bean rootworm (western bean cutworm)), lymantria dispar (Jeep's-web moths), myriopsis persicae Lyonetia clerkella (apple leaf miner), mahasena corbetti (oil palm bag moth) 3929 (oil palm bag worm), lophaneroides oleracea spp, cabbage looper Mamestra brassicae (cabbage armyworm), bean pod borer Maruca testulalis (bean pod borer) bag moth (metasa plana), mythimna unipuncta (true armyworm), mythimna separate (Walker) armyworm, neoleucinodes elegantalis, three-point water moth (Nymphula depunctalis), winter geometrid (Operophthera brumata), corn borer (Ostrinia nubilalis) (european corn borer (European corn borer)), oxydia vesulia, pandemis cerasana (leaf roller (common currant tortrix)), apple brown moth (Pandemis heparana) (brown apple tortrix), african damole butterfly (Papilio demodocus), red bell moth (Pectinophora gossypiella) (pink bollworm), peridroma species (Peridroma spp.) (rootworm), heteroblack tiger (Peridroma saia) (heterocolor rootworm (variegated cutworm)) The plant species may be selected from the group consisting of Phantom candidus (Perileucoptera coffeella) (white coffee leaf miner (white coffee leafminer)), phantom tuberosus (Phthorimaea operculella) (potato tuber moth), phantom citrus leaf miner (Phyllocnisitis citrella), philippine (Phyllonorycter spp.), pinceros rapae, spodoptera frugiperda (Plathypena scabra), india Gu Bane (Plodia interpunctella), cyperus rotundus (P.sub.f.) diamondback moth (Plutella xylostella), polychrosis viteana (grape leaf moth), fruit moth (Prays endocarpa), olive moth (Prays olee), pseudolites (olive moth), pseudolites sp (noctuid), pseudaletia unipunctata (armyworm), fruit moth (armyworm) soybean noctuid (Pseudoplusia includes) (, inchworm (rachiplus nu), chilo suppressalis Chilo suppressalis (Walker), trypanyza incertulas (Scirpophaga incertulas), spodoptera species (Sesamia spp.) (stem borers), spodoptera exigua (Sesamia insolens) (pink rice stem borer), phophophophophophophophophoma sphaeroides (Sesamia nonagrioides), brown moth (setoa nitens), wheat moth (Sitotroga cerealella) (Angoumois grain moth), grape armyworm (Sparganothis pilleriana), spodoptera species (Spodoptera spp.)), beet armyworm (Spodoptera exigua), spodoptera frugiperda (Spodoptera fugiperda), southern Spodoptera exigua (Spodoptera oridania) (southern Spodoptera exigua (southern armyworm)) A synanthus spp, thesla basialides, thermisia gemmatalis, clothes moth Tineola bisselliella (Spodoptera frugiperda webbing clothes moth), trichoplusia ni (Trichoplusia ni), and tomato spotted fly Tuta (Absolute);
The hemipteran (Hemiptera) pests include: stinkbug (Acrosternum hilare) (green stinkbug), american Gu Changchun (Blissus leucopterus) (green bug), lygus (Calocoris norvegicus), lygus lucorum (Acrosternum hilare) (green stinkbug), american Gu Changchun (Blissus leucopterus) (green bug), potato stinkbug (Calocoris norvegicus), aphids (Aphis), scale, whiteflies (white flies), leafhoppers (leaf hoppers), pea aphids (Acrythosiphon pisum) (pea aphids), sphagnum species (Adelges spp), cabbage whiteflies (Aleurodes proletella), spiralis (Aleurodicus disperses), silk white flies (Aleurothrixus floccosus) (cotton lice (white fly)); the species of the genus Lecanis (Aluacaspis spp.), the species of the genus Lecanis (Aonidiella aurantii) aphid (Aphis spp.), aphis gossypii (Aphis gossypii), aphis pomi (Aphis pomi), aphis long whisker aphid (Aulacorthum solani) (foxglove aphid), the species of the genus Bemisia (Bemisia spp.) (Bemisia) and white fly (Trialeurodea vaporirum) (Westwood)), white fly (Bemisia argentifolii), white fly (Bemisia tabaci) (sweet potato white fly), white fly (Brachycolus noxius), white fly (Brachycorynella asparagi) (asparagus Aphis), brevennia reehi, cabbage aphid (Brevicoryne brassicae), the plant species may be selected from the group consisting of Ericerus species (Ceroplastes deck), ericerus pela (Ceroplastes rubens) (Ericerus pela), ericerus species (Chionasepis deck), ericerus species (Chrysomphalus deck), plantago (Dysaphis plantaginea) (red apple Aphis), empoasca species (Empoasca deck), aphis apple (Eriosoma lanigerum), ericerus praecox (Icerya purchasi) (Ericerus) and (cottony cushion scale)), ericerus mangifer (Idioscopus nitidulus) (Mangifera cicada (mangius) and (Laodelphax striatellus), ericerus pela species (Lepidosporus p), aleuropa species (Macosporus frondosus deck), myzu species (Lepidus Macrosiphum euphorbiae), myzu (Macrosiphum granarium), rosa (Endocarpifolia) species (394), tozu (Macrosteles quadrilineatus), torpedon (P) species (P.persica), neisseria (P.sp) (35), neisseria (P.tius (P.tikokurzeri) (35), neisseria (P.tikoku) (35), neisseria (P.tikokurz) (35), neisseria (P.p.brueckii) (35), and (P.brueckea (P.tikoku (P.i) and (P.cucu) The species of mealybugs (Physokermes piceae) (spruce round scale), gensis (placocucus sp.) (mealybugs), mealybugs (pseudococucus sp.) (mealybugs), mealybugs (Pseudococcus brevipes) (pineapple mealybugs (pine apple mealybug)), pear garden scale (Quadraspidiotus perniciosus) (pear garden scale (San Jose scale)), mealybugs (sphaera sativa) species (rhopalosiphon sp.)), corn aphids (Rhopalosiphum maida) (corn She Ya (corn leaf aphid)), meadow corn spillway aphids (Rhapalosiphum padi) (oat bird-cherry plant), mealybugs (Saissetia sp.), eleusine meyeriana (Schizaphis graminum) (green aphid (gretag)), wheat long tube (sitoav 35ae) 26), white beetle (yellow meadow sp.) (yellow mealybugs), white beetle sp.) (yellow mealybugs), white beetles sp.) (yellow mealybugs, yellow mealybugs (yellow beetles);
Still further, the lepidoptera pests include plutella xylostella, asparagus caterpillar, prodenia litura, chilo suppressalis, armyworm, cotton bollworm and cabbage caterpillar; the hemipteran pests comprise whiteflies, rice planthoppers and aphids.
An insecticidal composition comprising bistrifluron is applied in an effective amount to the pest to be controlled or to its growth medium.
The application has the advantages that:
(1) After being compounded with any one of spinosad, dinotefuran, flonicamid and nitenpyram, the compound pesticide has obvious synergistic effect in a certain range;
(2) The dosage of the medicament is reduced, the residual quantity of the pesticide on crops is reduced, and the environmental pollution is lightened;
(3) The insecticidal composition expands the insecticidal spectrum and has better control effect on various pests.
Detailed Description
The present application is illustrated by the following specific examples in order to make the technical solution, objects and advantages of the present application more apparent, but the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. While the application has been described in detail with respect to the general description and specific embodiments thereof, it will be apparent to those skilled in the art that various modifications and improvements can be made therein, and equivalents thereof may fall within the scope of the application.
Formulation preparation examples:
example 1:28% Ditrifluoracene spinosad suspension (2:5)
The formula comprises the following components: the balance of 8% of bistrifluron, 20% of spinosad, 1% of isomeric tridecanol polyoxyethylene ether, 1% of naphthalene sulfonate formaldehyde condensate, 3% of styrol polyoxyethylene ether phosphate, 2% of alkylaryl polyoxyethylene ether polyoxypropylene ether, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 5% of propylene glycol, 0.2% of potassium benzoate, 0.5% of silicone oil and deionized water.
The preparation method comprises the following steps: according to the formula proportion, the active ingredients, the surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, high-speed shearing and wet sanding are carried out, and finally, the suspending agent product is obtained through homogenizing and filtering.
Example 2:20% Ditrifluoracene spinosad suspension (3:1)
The formula comprises the following components: the balance of 15 percent of bistrifluron, 5 percent of spinosad, 3 percent of alkylphenol ethoxylate, 4 percent of alkylphenol ethoxylate phosphate, 1 percent of polycarboxylic acid sodium salt, 0.25 percent of xanthan gum, 5 percent of glycerol, 0.1 percent of sodium benzoate, 0.5 percent of silicone oil and deionized water are added according to the weight percentage.
The preparation method comprises the following steps: as in example 1.
Example 3:18% Ditrifluoracene spinosad suspension (1:8)
The formula comprises the following components: the balance of 2 percent of bistrifluron, 16 percent of spinosad, 1 percent of succinate sulfonate, 3 percent of styrylphenol polyoxyethylene ether sulfate, 2 percent of alkylaryl polyoxyethylene ether polyoxypropylene ether, 1 percent of lignin sulfonate, 1.5 percent of magnesium aluminum silicate, 0.1 percent of carboxyethyl cellulose, 0.1 percent of xanthan gum, 1 percent of sodium sorbate, 5 percent of ethylene glycol, 0.5 percent of silicone oil and deionized water are added according to the weight percentage.
The preparation method comprises the following steps: as in example 1.
Example 4:20% bistrifluron and spinosad water dispersible granule (1:3)
The formula comprises the following components: the pesticide comprises, by weight, 5% of bistrifluron, 15% of spinosad, 10% of lignosulfonate, 4% of sodium dodecyl benzene sulfonate, 5% of white carbon black, 30% of starch and the balance of kaolin.
The preparation method comprises the following steps: according to the formula proportion of the embodiment, the active ingredients are added into a carrier, and the surfactant and other functional additives are added into the carrier, mixed, crushed by air flow, added with 10 to 25 percent of water, and then kneaded, granulated, dried and screened to prepare the water dispersible granule product.
Example 5:
30% bistrifluron and spinosad water dispersible granule (1:2)
The formula comprises the following components: the pesticide comprises, by weight, 10% of bistrifluron, 20% of spinosad, 8% of sodium lignin sulfonate, 2.5% of nekal BX, 1.5% of sodium dodecyl benzene sulfonate, 5% of ammonium sulfate and the balance of kaolin.
The preparation method comprises the following steps: same as in example 4.
Example 6:35% bistrifluron and spinosad water dispersible granule (6:1)
The formula comprises the following components: the pesticide comprises, by weight, 30% of bistrifluron, 5% of spinosad, 8% of naphthalene sulfonate formaldehyde condensate, 2% of sodium polycarboxylate, 3% of nekal BX and the balance of kaolin.
The preparation method comprises the following steps: same as in example 4.
Example 7:21% Di-trifluoracer dinotefuran suspension (3:4)
The formula comprises the following components: 9% of bistrifluron, 12% of dinotefuran, 1% of sodium dodecyl sulfate, 1% of naphthalene sulfonate formaldehyde condensate, 3% of styrol polyoxyethylene ether phosphate, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 4% of propylene glycol, 0.01% of benzisothiazolinone potassium, 0.5% of silicone oil and the balance of deionized water.
The preparation method comprises the following steps: as in example 1.
Example 8:20% Ditrifluoracer dinotefuran suspension (1:3)
The formula comprises the following components: the balance is prepared from 5% of bistrifluron, 15% of dinotefuran, 0.5% of alkylphenol polyoxyethylene, 4% of alkylphenol polyoxyethylene phosphate, 1% of sodium lignin sulfonate, 0.25% of xanthan gum, 5% of glycerol, 0.1% of sodium benzoate, 0.5% of silicone oil and deionized water according to weight percentage.
The preparation method comprises the following steps: as in example 1.
Example 9:30% Di-trifluoracer dinotefuran suspension (1:2)
The formula comprises the following components: the weight percentages of the components are 10 percent of bistrifluron, 20 percent of dinotefuran, 1 percent of fatty alcohol polyoxyethylene ether, 3 percent of alkylaryl polyoxyethylene ether polyoxypropylene ether, 2 percent of styrol polyoxyethylene ether sulfate ester, 1 percent of polycarboxylic acid sodium salt, 1.5 percent of magnesium aluminum silicate, 0.1 percent of carboxyethyl cellulose, 1 percent of sodium sorbate, 5 percent of ethylene glycol, 0.5 percent of silicone oil and the balance of deionized water.
The preparation method comprises the following steps: as in example 1.
Example 10:20% Ditrifluoracer dinotefuran water dispersible granule (1:3)
The formula comprises the following components: the balance is 5 percent of bistrifluron, 15 percent of dinotefuran, 10 percent of lignosulfonate, 4 percent of sodium dodecyl benzene sulfonate, 2 percent of sodium dodecyl sulfate, 5 percent of white carbon black, 30 percent of starch and kaolin.
The preparation method comprises the following steps: same as in example 4.
Example 11:30% bistrifluron dinotefuran water dispersible granule (1:2)
The formula comprises the following components: the weight percentage is that 10 percent of bistrifluron, 20 percent of dinotefuran, 8 percent of sodium lignin sulfonate, 2.5 percent of nekal BX, 1.5 percent of sodium dodecyl benzene sulfonate, 5 percent of white sugar and kaolin are used for the balance.
The preparation method comprises the following steps: same as in example 4.
Example 12:40% bistrifluron dinotefuran water dispersible granule (1:3)
The formula comprises the following components: the balance is 10 percent of bistrifluron, 30 percent of dinotefuran, 8 percent of naphthalene sulfonate formaldehyde condensate, 2 percent of sodium polycarboxylate, 2 percent of sodium dodecyl sulfate, 10 percent of ammonium sulfate and starch in percentage by weight.
The preparation method comprises the following steps: same as in example 4.
Example 13:24% Ditrifluoracer dinotefuran dispersible oil suspension (1:5)
The formula comprises the following components: the pesticide composition comprises, by weight, 4% of bistrifluron, 20% of dinotefuran, 2% of lignin sulfonate, 14% of alkylaryl polyoxyethylene polyoxypropylene ether, 3% of fatty alcohol polyoxyethylene ether, 2% of calcium dodecyl benzene sulfonate, 1% of silicon dioxide, 1% of organic bentonite, 20% of 200# solvent oil and the balance of methyl oleate.
The preparation method comprises the following steps: and (3) sequentially placing the active ingredients, the surfactant and other functional additives in a reaction kettle according to the formula proportion, adding oil, uniformly mixing, shearing at a high speed, sanding by a wet method, and finally homogenizing and filtering to obtain the dispersible oil suspending agent product.
Example 14:20% Ditrifluoracer dinotefuran dispersible oil suspension (1:3)
The formula comprises the following components: the balance of the composition comprises, by weight, 5% of bistrifluron, 15% of dinotefuran, 4% of alkylphenol ethoxylates, 12% of castor oil polyoxyethylene ethers, 1% of calcium dodecyl benzene sulfonate, 1% of sodium polycarboxylate, 1% of naphthalene sulfonate formaldehyde condensate and the balance of soybean oil.
The preparation method comprises the following steps: same as in example 13.
Example 15:15% Di-trifluoracer dinotefuran dispersible oil suspension (1:2)
The formula comprises the following components: the pesticide composition comprises, by weight, 5% of bistrifluron, 10% of dinotefuran, 1% of fatty alcohol polyoxyethylene ether, 15% of polyoxyethylene sorbitan monooleate, 2% of calcium dodecyl benzene sulfonate, 2% of succinate sulfonate, 1.5% of magnesium aluminum silicate, 1% of Teck 869 and the balance of corn oil.
The preparation method comprises the following steps: same as in example 13.
Example 16:30% Di-trifluoracer dinotefuran emulsifiable concentrate (5:1)
The formula comprises the following components: according to the weight percentage, 25 percent of bistrifluron, 5 percent of dinotefuran, 15 percent of DMF, 14 percent of styrylphenol polyoxyethylene ether, 2 percent of calcium dodecyl benzene sulfonate, 20 percent of propylene carbonate and the balance of dimethylbenzene are added.
The preparation method comprises the following steps: according to the formula proportion, adding the metered active ingredients, the solvent and the cosolvent into a blending kettle, stirring to dissolve the active ingredients, adding the emulsifier, supplementing the rest with the rest solvent, stirring uniformly in the stirring kettle, and filtering to obtain the emulsifiable concentrate.
Example 17:18% Di-trifluoracer dinotefuran emulsifiable concentrate (2:1)
The formula comprises the following components: according to the weight percentage, 12 percent of bistrifluron, 6 percent of dinotefuran, 20 percent of N-methyl pyrrolidone, 14 percent of alkylphenol ethoxylates, 2 percent of calcium dodecyl benzene sulfonate, 10 percent of DMF and the balance of methyl oleate are added.
The preparation method comprises the following steps: same as in preparation example 16.
Example 18:10% Di-trifluoracer dinotefuran emulsifiable concentrate (1:1)
The formula comprises the following components: 5% of bistrifluron, 5% of dinotefuran, 15% of EO/PO block copolymer, 15% of acetophenone, 10% of N-octyl pyrrolidone, 1% of calcium dodecyl benzene sulfonate and the balance of trimethylbenzene.
The preparation method comprises the following steps: same as in preparation example 16.
Example 19:24% Ditriflumuron flonicamid suspension (1:5)
The formula comprises the following components: the weight percentages of the components are 4 percent of bistrifluron, 20 percent of flonicamid, 1 percent of fatty alcohol polyoxyethylene ether, 1 percent of polycarboxylic acid sodium salt, 3 percent of styrol polyoxyethylene ether sulfate, 2 percent of alkylaryl polyoxyethylene ether polyoxypropylene ether, 0.5 percent of magnesium aluminum silicate, 0.20 percent of xanthan gum, 4 percent of propylene glycol, 0.2 percent of potassium benzoate, 0.5 percent of silicone oil and the balance of deionized water.
The preparation method comprises the following steps: as in example 1.
Example 20:20% Ditriflumuron flonicamid suspension (1:3)
The formula comprises the following components: according to the weight percentage, 5 percent of bistrifluron, 15 percent of flonicamid, 1 percent of isomeric alcohol polyoxyethylene ether, 4 percent of alkylphenol polyoxyethylene ether phosphate, 1 percent of lignosulfonate, 2 percent of alkylaryl polyoxyethylene ether polyoxypropylene ether, 0.25 percent of xanthan gum, 5 percent of glycerol, 0.1 percent of sodium benzoate, 0.5 percent of silicone oil and deionized water are used for the balance.
The preparation method comprises the following steps: as in example 1.
Example 21:25% Ditriflumuron flonicamid suspension (3:2)
The formula comprises the following components: the weight percentages of the components are 15 percent of bistrifluron, 10 percent of flonicamid, 1 percent of fatty alcohol polyoxyethylene ether, 2 percent of alkylaryl polyoxyethylene ether polyoxypropylene ether, 1 percent of naphthalene sulfonate formaldehyde condensate, 4 percent of styrol polyoxyethylene ether phosphate, 1.5 percent of magnesium aluminum silicate, 0.1 percent of carboxyethyl cellulose, 1 percent of sodium sorbate, 5 percent of ethylene glycol, 0.5 percent of silicone oil and the balance of deionized water.
The preparation method comprises the following steps: as in example 1.
Example 22:25% bistrifluron and flonicamid water dispersible granule (2:3)
The formula comprises the following components: the pesticide comprises, by weight, 10% of bistrifluron, 15% of flonicamid, 10% of lignosulfonate, 4% of sodium dodecyl benzene sulfonate, 2% of sodium dodecyl sulfate, 30% of starch and the balance of kaolin.
The preparation method comprises the following steps: same as in example 4.
Example 23:30% bistrifluron and flonicamid water dispersible granule (1:2)
The formula comprises the following components: the pesticide composition comprises, by weight, 10% of bistrifluron, 20% of flonicamid, 8% of sodium lignin sulfonate, 3% of naphthalene sulfonate formaldehyde condensate, 2.5% of nekal BX, 1.5% of sodium dodecyl benzene sulfonate, 10% of ammonium sulfate and the balance of kaolin.
The preparation method comprises the following steps: same as in example 4.
Example 24:40% bistrifluron and flonicamid water dispersible granule (1:3)
The formula comprises the following components: the balance is 10 percent of bistrifluron, 30 percent of flonicamid, 10 percent of naphthalene sulfonate formaldehyde condensate, 2 percent of sodium polycarboxylate, 4 percent of sodium dodecyl sulfate and light calcium carbonate in percentage by weight.
The preparation method comprises the following steps: same as in example 4.
Example 25:27% Ditrifluramide-flonicamid dispersible oil suspension (1:8)
The formula comprises the following components: 3% of bistrifluron, 24% of flonicamid, 1% of naphthalene sulfonate formaldehyde condensate, 12% of alkylaryl polyoxyethylene polyoxypropylene ether, 3% of fatty alcohol polyoxyethylene ether, 2% of calcium dodecyl benzene sulfonate, 1% of silicon dioxide, 1% of organic bentonite and the balance of methyl oleate;
the preparation method comprises the following steps: same as in example 13.
Example 26:20% Ditriflumuron flonicamid dispersible oil suspension (1:3)
The formula comprises the following components: according to the weight percentage, 5 percent of bistrifluron, 15 percent of flonicamid, 4 percent of alkylphenol ethoxylate, 10 percent of castor oil polyoxyethylene, 1 percent of calcium dodecyl benzene sulfonate, 1 percent of sodium salt of polycarboxylic acid and the balance of soybean oil are added;
The preparation method comprises the following steps: same as in example 13.
Example 27:15% Ditrifluramide-flonicamid dispersible oil suspension (1:2)
The formula comprises the following components: according to the weight percentage, 5 percent of bistrifluron, 10 percent of flonicamid, 1 percent of fatty alcohol polyoxyethylene ether, 14 percent of polyoxyethylene sorbitan monooleate, 2 percent of calcium dodecyl benzene sulfonate, 1 percent of succinate sulfonate, 1.5 percent of magnesium aluminum silicate, 1 percent of Teck 869 and the balance of corn oil are added;
the preparation method comprises the following steps: same as in example 13.
Example 28:20% Ditrifluoracer flonicamid emulsifiable concentrate (3:1)
The formula comprises the following components: 15% of bistrifluron, 5% of flonicamid, 10% of dimethyl sulfoxide, 13% of styrylphenol polyoxyethylene ether, 2% of calcium dodecyl benzene sulfonate, 20% of propylene carbonate and the balance of dimethylbenzene.
The preparation method comprises the following steps: same as in example 16.
Example 29:18% bistrifluron-flonicamid emulsifiable concentrate (2:1)
The formula comprises the following components: 12% of bistrifluron, 6% of flonicamid, 20% of propylene glycol methyl ether, 15% of alkylphenol ethoxylates, 2% of calcium dodecyl benzene sulfonate, 10% of DMF and the balance of methyl oleate.
The preparation method comprises the following steps: same as in preparation example 16.
Example 30:10% Di-triflumuron-flonicamid emulsifiable concentrate (1:1)
The formula comprises the following components: according to the weight percentage, 5 percent of bistrifluron, 5 percent of flonicamid, 15 percent of EO/PO block copolymer, 15 percent of acetophenone, 10 percent of propylene glycol methyl ether, 1 percent of calcium dodecyl benzene sulfonate and 100 percent of trimethylbenzene are complemented.
The preparation method comprises the following steps: same as in preparation example 16.
Example 31:20% bistrifluron nitenpyram water dispersible granule (1:3)
The formula comprises the following components: the balance is 5 percent of bistrifluron, 15 percent of nitenpyram, 10 percent of dispersant NNO, 2 percent of sodium dodecyl sulfate, 4 percent of sodium dodecyl benzene sulfonate, 30 percent of starch and light calcium carbonate.
The preparation method comprises the following steps: same as in example 4.
Example 32:30% bistrifluron nitenpyram water dispersible granule (1:2)
The formula comprises the following components: the pesticide comprises, by weight, 10% of bistrifluron, 20% of nitenpyram, 10% of sodium lignin sulfonate, 2.5% of nekal BX, 1.5% of sodium dodecyl benzene sulfonate, 10% of glucose and the balance of starch.
The preparation method comprises the following steps: same as in example 4.
Example 33:40% bistrifluron nitenpyram water dispersible granule (1:3)
The formula comprises the following components: the weight percentages of the components are 10 percent of bistrifluron, 30 percent of nitenpyram, 4 percent of naphthalene sulfonate formaldehyde condensate, 10 percent of sodium polycarboxylate, 2 percent of sodium dodecyl sulfate, 5 percent of white carbon black and kaolin, and the balance is made up.
The preparation method comprises the following steps: same as in example 4.
Example 34:14% Ditriflumuron nitenpyram emulsifiable concentrate (2:5)
The formula comprises the following components: according to the weight percentage, 4% of bistrifluron, 10% of nitenpyram, 15% of EO/PO block copolymer, 10% of dimethyl sulfoxide, 10% of propylene glycol methyl ether, 1% of calcium dodecyl benzene sulfonate and 100% of trimethylbenzene are complemented.
The preparation method comprises the following steps: same as in example 16.
Example 35:18% bistrifluron nitenpyram emulsifiable concentrate (5:1)
The formula comprises the following components: 15% of bistrifluron, 3% of nitenpyram, 30% of acetophenone, 10% of castor oil polyoxyethylene ether, 5% of fatty alcohol polyoxyethylene ether, 2% of calcium dodecyl benzene sulfonate and 100% of methyl oleate.
The preparation method comprises the following steps: same as in example 16.
Example 36:15% Ditriflumuron nitenpyram emulsifiable concentrate (1:2)
The formula comprises the following components: according to the weight percentage, 5% of bistrifluron, 10% of nitenpyram, 30% of propylene carbonate, 10% of polyoxyethylene sorbitan monooleate, 5% of isomeric tridecanol polyoxyethylene ether, 1% of calcium dodecyl benzene sulfonate and 100% of rosin-based vegetable oil are complemented.
The preparation method comprises the following steps: same as in example 16.
Indoor biological Activity assay
Indoor test 1 Combined toxicity of Di-Triflufenoxuron and spinosad to Chilo suppressalis and myxoma
The test method comprises the following steps: leaf dipping method;
test agent: 94.7% of bistrifluron (supplied by the group development center) and 92% of spinosad (supplied by zilu pharmaceutical (inner mongolia) limited).
Test targets and age: chilo suppressalis (Chilo suppressalis (Walker)) in the early three years; the armyworm (Mythimna separata (Walker)) is a population which is continuously bred and propagated indoors by a research and development center at the early three-age stage.
The larvae adopt fresh cane shoot segments as feed. Feeding conditions: the temperature of the constant temperature insect-culturing room is 25+/-1 ℃, the relative humidity is 65+/-5%, and the illumination L is D=14:10 h.
The measuring method comprises the following steps: the mother solution of single agent is prepared respectively, 5 groups of proportions are designed according to the mixing purpose and the medicament activity, and 5 series of mass concentrations of each single agent and each group of proportion mixture are prepared according to an equal ratio method. Each treatment was repeated 4 times for 20 test insects. Placing the mixture in a constant temperature insect-culturing room with the temperature of 25+/-1 ℃ and the relative humidity of 65+/-5 percent, and irradiating the mixture for L with light for D=14:10 h.
Examples reference is made to NY/T1154.7-2006 section 7 pesticide, guidelines for biological assay in pesticide laboratory: mixed combination action determination, NY/T1154.14-2008 pesticide section 14, biological assay test guidelines in pesticide Chamber: leaf dipping method.
Data statistics and analysis:
The death of the test insects is checked 72 hours after the treatment, and the total insects and the dead insects are respectively recorded. From the survey data, corrected mortality for each treatment was calculated. Calculating according to formulas (1) and (2), wherein the calculation results are reserved to two positions after decimal point:
wherein:
p-mortality in percent (%);
k-represents the number of dead insects in units of heads;
n-represents the total number of insects treated in units of heads.
Wherein:
P 1 -correct mortality in percent (%);
P t mortality rate in percent (%);
P 0 blank mortality in percent (%).
If the control mortality is less than 5%, correction is not needed; the control mortality is between 5% and 20%, and correction is carried out according to the formula (2); control mortality was > 20% and the test was reworked.
And processing the data by adopting a probability value analysis method. Can be analyzed by IBM SPSS Statistics 20 statistical analysis system to obtain virulence regression line and LC 50 Value and 95% confidence limit and correlation coefficient R thereof 2 The activity of the test agent on the biological test material was evaluated.
The co-toxicity coefficient (CTC value) of the mixture is calculated according to the formulas (3), (4) and (5):
wherein:
ati—actual measured virulence index of the mixture;
S-LC of Standard insecticide 50 Milligrams per liter (mg/L);
M—LC of the mixture 50 Units are milligrams per liter (mg/L).
TTI＝TI A * P A +TI B * P B ·······(4)
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A -a medicament virulence index;
P A -the percentage of agent a in the mix, in percent (%);
TI B -B agent virulence index;
P B the percentage of the B medicament in the mixture is expressed as percentage (%).
Wherein:
ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The compound co-toxicity coefficient CTC is more than or equal to 120 and shows synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
The test results are shown in tables 1 and 2.
TABLE 1 Combined virulence of Di-trifluoracene and spinosad to Chilo suppressalis
As shown in the table, when the mass ratio of the bistrifluron to the spinosad is 1:30-25:1, the co-toxicity coefficient is greater than 120, and the synergy is shown; when the mass ratio of the bistrifluron to the spinosad is 1:10-25:1, the co-toxicity coefficient is greater than 150, and the synergy is remarkable.
TABLE 2 Combined virulence of Di-trifluoracer and spinosad for armyworm
As shown in the table, when the mass ratio of the bistrifluron to the spinosad is 1:10-8:1, the co-toxicity coefficients of the bistrifluron and the spinosad are all greater than 120, and the bistrifluron and spinosad show synergistic effect; when the mass ratio of the bistrifluron to the spinosad is 1:8-6:1, the co-toxicity coefficient is greater than 150, and the synergy is remarkable.
Indoor test 2 combination toxicity of Di-trifluoracer and dinotefuran to whiteflies
The test method comprises the following steps: the leaf soaking method by agar moisture preservation.
Test target: the greenhouse trialeurodes vaporariorum (Trialeurodea vaporirum (Westwood)) adults, and the source of the test insects is a population which is continuously bred and propagated for multiple generations indoors by a group development center.
Test agent: 94.7% of the bistrifluramide crude drug and 98% of the dinotefuran crude drug are provided by the group development center.
The test steps are as follows:
(1) Preparing liquid agar:
putting agar powder into a triangular flask, adding water, transferring into a microwave oven, and heating until agar is completely dissolved. After cooling, 2mL of liquid agar is sucked by a pipette, the liquid agar is added to the bottom of the flat-bottom glass tube, and after the liquid agar is cooled and solidified, the tube wall steam volatilizes cleanly for later use. And in the cooling process, the agar is prevented from being stained with the pipe wall and solidified agar to generate bubbles.
(2) And (3) preparation of a medicament: the mother solution of single agent is prepared respectively, 5 groups of proportions are designed according to the mixing purpose and the medicament activity, and 5 series of mass concentrations of each single agent and each group of proportion mixture are prepared according to an equal ratio method.
(3) And (3) medicament treatment:
fresh and flat cucumber leaves are punched into leaf discs by a puncher. And immersing the leaf disc into the liquid medicine to be detected for 10s, taking out, airing, spreading the leaf in a flat-bottom glass tube with agar in which the front face of the leaf is downward by using tweezers, and tightly attaching the leaf disc to the agar and the tube wall without leaving gaps. Treatments were repeated 4 times for each group and treatments without agent were set as blank.
(3) And (3) insect inoculation: through the glass tube paved with leaf discs, the leaf discs are inclined on cucumber leaves for culturing whiteflies, the leaves are gently beaten, so that the whiteflies enter the glass tube, 20 whiteflies are connected into each tube, the mouth of the tube faces downwards, the whiteflies are placed into the leaf discs at the bottom of the tube, and the position, which is about 15mm away from the bottom of the tube, of the cotton plug is plugged, so that the whiteflies are forcedly positioned on the leaf discs at the bottom of the tube.
(4) Feeding and observing:
the treated test insects are inverted to a temperature of (25+/-1) DEG C and a relative humidity of 65% +/-5%, and the light period is L:D=14:10 h, and are fed and observed.
(5) Investigation:
the number of live insects in each tube is checked and recorded one by one 48 hours after the treatment, and the death is considered if the test insects are motionless or can not normally act. From the survey data, corrected mortality for each treatment was calculated. The test data calculation formula is as described above.
The test results are shown in Table 3.
TABLE 3 combination toxicity of Di-trifluoracer and dinotefuran to whiteflies
From the table, when the mass ratio of the bistrifluron to the dinotefuran is 1:15-10:1, the co-toxicity coefficients of the bistrifluron and the dinotefuran are all greater than 120, and the bistrifluron and the dinotefuran show synergistic effect; when the mass ratio of the bistrifluron to the dinotefuran is 2:9-10:1, the co-toxicity coefficient is greater than 150, and the synergy is remarkable.
Indoor test 3 Combined toxicity of Di-Triflufenoxuron and flonicamid on Bemisia
The test method comprises the following steps: the leaf soaking method by agar moisture preservation.
Test target: the greenhouse trialeurodes vaporariorum (Trialeurodea vaporirum (Westwood)) adults, and the source of the test insects is a population which is continuously bred and propagated for multiple generations indoors by a group development center.
Test agent: 94.7% of the bistriflumuron technical and 96% of the flonicamid technical are provided by the group development center.
The test steps are as follows:
(1) Preparing liquid agar:
putting agar powder into a triangular flask, adding water, transferring into a microwave oven, and heating until agar is completely dissolved. After cooling, 2mL of liquid agar is sucked by a pipette, the liquid agar is added to the bottom of the flat-bottom glass tube, and after the liquid agar is cooled and solidified, the tube wall steam volatilizes cleanly for later use. And in the cooling process, the agar is prevented from being stained with the pipe wall and solidified agar to generate bubbles.
(2) And (3) preparation of a medicament: the mother solution of single agent is prepared respectively, 5 groups of proportions are designed according to the mixing purpose and the medicament activity, and 5 series of mass concentrations of each single agent and each group of proportion mixture are prepared according to an equal ratio method.
(3) And (3) medicament treatment:
fresh and flat cucumber leaves are punched into leaf discs by a puncher. And immersing the leaf disc into the liquid medicine to be detected for 10s, taking out, airing, spreading the leaf in a flat-bottom glass tube with agar in which the front face of the leaf is downward by using tweezers, and tightly attaching the leaf disc to the agar and the tube wall without leaving gaps. Treatments were repeated 4 times for each group and treatments without agent were set as blank.
(3) And (3) insect inoculation: through the glass tube paved with leaf discs, the leaf discs are inclined on cucumber leaves for culturing whiteflies, the leaves are gently beaten, so that the whiteflies enter the glass tube, 20 whiteflies are connected into each tube, the mouth of the tube faces downwards, the whiteflies are placed into the leaf discs at the bottom of the tube, and the position, which is about 15mm away from the bottom of the tube, of the cotton plug is plugged, so that the whiteflies are forcedly positioned on the leaf discs at the bottom of the tube.
(4) Feeding and observing:
the treated test insects are inverted to a temperature of (25+/-1) DEG C and a relative humidity of 65% +/-5%, and the light period is L:D=14:10 h, and are fed and observed.
(5) Investigation:
the number of live insects in each tube is checked and recorded one by one 48 hours after the treatment, and the death is considered if the test insects are motionless or can not normally act. From the survey data, corrected mortality for each treatment was calculated. The test data calculation formula is as described above.
The test results are shown in Table 4.
TABLE 4 Combined toxicity of Di-trifluramide and flonicamid to whiteflies
As shown in the table, when the mass ratio of the bistrifluron to the flonicamid is 1:25-10:1, the co-toxicity coefficients of the bistrifluron and the flonicamid are all greater than 120, and the bistrifluron and flonicamid show synergistic effect; when the mass ratio of the bistrifluron to the flonicamid is 2:15-2:3, the co-toxicity coefficient is greater than 150, and the synergy is remarkable.
Indoor test 4 Combined toxicity of Di-triflumuron and nitenpyram on whiteflies
The test method comprises the following steps: the leaf soaking method by agar moisture preservation.
Test target: the greenhouse trialeurodes vaporariorum (Trialeurodea vaporirum (Westwood)) adults, and the source of the test insects is a population which is continuously bred and propagated for multiple generations indoors by a group development center.
Test agent: 94.7% of the bistriflumuron technical and 98% of the nitenpyram technical are provided by the group development center.
The test steps are as follows:
(1) Preparing liquid agar:
putting agar powder into a triangular flask, adding water, transferring into a microwave oven, and heating until agar is completely dissolved. After cooling, 2mL of liquid agar is sucked by a pipette, the liquid agar is added to the bottom of the flat-bottom glass tube, and after the liquid agar is cooled and solidified, the tube wall steam volatilizes cleanly for later use. And in the cooling process, the agar is prevented from being stained with the pipe wall and solidified agar to generate bubbles.
(2) And (3) preparation of a medicament: the mother solution of single agent is prepared respectively, 5 groups of proportions are designed according to the mixing purpose and the medicament activity, and 5 series of mass concentrations of each single agent and each group of proportion mixture are prepared according to an equal ratio method.
(3) And (3) medicament treatment:
fresh and flat cucumber leaves are punched into leaf discs by a puncher. And immersing the leaf disc into the liquid medicine to be detected for 10s, taking out, airing, spreading the leaf in a flat-bottom glass tube with agar in which the front face of the leaf is downward by using tweezers, and tightly attaching the leaf disc to the agar and the tube wall without leaving gaps. Treatments were repeated 4 times for each group and treatments without agent were set as blank.
(3) And (3) insect inoculation: through the glass tube paved with leaf discs, the leaf discs are inclined on cucumber leaves for culturing whiteflies, the leaves are gently beaten, so that the whiteflies enter the glass tube, 20 whiteflies are connected into each tube, the mouth of the tube faces downwards, the whiteflies are placed into the leaf discs at the bottom of the tube, and the position, which is about 15mm away from the bottom of the tube, of the cotton plug is plugged, so that the whiteflies are forcedly positioned on the leaf discs at the bottom of the tube.
(4) Feeding and observing:
the treated test insects are inverted to a temperature of (25+/-1) DEG C and a relative humidity of 65% +/-5%, and the light period is L:D=14:10 h, and are fed and observed.
(5) Investigation:
the number of live insects in each tube is checked and recorded one by one 48 hours after the treatment, and the death is considered if the test insects are motionless or can not normally act. From the survey data, corrected mortality for each treatment was calculated. The test data calculation formula is as described above.
The test results are shown in Table 5.
TABLE 5 Combined toxicity of Di-triflumuron and nitenpyram on whiteflies
As shown in the table, when the mass ratio of the bistrifluron to the nitenpyram is 1:10-7:1, the co-toxicity coefficients of the bistrifluron and the nitenpyram are all greater than 120, and the bistrifluron and the nitenpyram show synergistic effect; when the mass ratio of the bistrifluron to the nitenpyram is 1:7-5:1, the co-toxicity coefficients of the bistrifluron and the nitenpyram are all more than 150, and the synergy is remarkable.
Example of field efficacy
Field test 1 field control effect of mixed pair myxoplasma of bistrifluron and spinosad
Test crop: corn;
test target: armyworm;
test site: qingdao city plain corn field;
test time: 2020, 6 months and 10 days;
test environment: the corn damage degree is consistent, the cultivation conditions of all the test communities are uniform, and the same fertilizer and water management level is adopted.
Test agent: 30% of double-trifluoracer-spinosad water dispersible granule (1:2), 20% of double-trifluoracer-spinosad water dispersible granule (1:3), 28% of double-trifluoracer-spinosad suspending agent (2:5), 42% of double-trifluoracer-spinosad water dispersible granule (4:3), 10% of double-trifluoracer-urea suspending agent and 25 g/L of spinosad suspending agent, wherein the medicaments are provided by a group research and development center.
The test method comprises the following steps: the pest is initially applied for 1 time.
Cell area and repetition: each cell was repeated 4 times at 20 square meters per process.
The investigation method comprises the following steps: sampling 5 points per cell, 1m per point 2 The number of the insect population is investigated before the application, the survival number of the armyworms is investigated 3 days and 7 days after the application, and the control effect of each treatment on the armyworms is calculated according to the investigation result.
Calculation formula and data analysis:
the result of the calculation retains two decimal places.
The test results are shown in Table 6.
Table 6 field control of armyworm by mixing bistrifluron and spinosad
As can be seen from the field efficacy test in Table 6, the mixed pesticide composition of the bistrifluron and spinosad has good control effect on armyworms. Wherein, the 28% suspending agent of the bistrifluron and spinosad (2:5) has the best control effect on armyworm at 7 days after the medicament, and the control effect is 90.85%.
Field test 2 field control effect of mixed bistrifluoracene and spinosad on chilo suppressalis
Test crop: rice;
test target: chilo suppressalis;
test site: qingdao city instant ink paddy field;
test time: 2020, 6 months and 10 days;
test environment: the rice damage degree is consistent, the cultivation conditions of all the test communities are uniform, and the same fertilizer and water management level is adopted.
Test agent: the pesticide composition comprises 30% of water dispersible granule of bistrifluron and spinosad (1:2), 20% of water dispersible granule of bistrifluron and spinosad (1:3), 28% of suspending agent of bistrifluron and spinosad (2:5), 10% of suspending agent of bistrifluron and 25 g/L spinosad, and the medicaments are provided by a group research and development center.
The test method comprises the following steps: the pest is initially applied for 1 time.
Cell area and repetition: each cell was repeated 4 times at 20 square meters per process.
The investigation method comprises the following steps: when the damage of the blank control area is obvious (10 d after the medicine), parallel jump sampling is adopted, 45 clusters of rice are investigated in each cell, and the withered heart rate and the death rate are counted.
Calculation formula and data analysis:
CK-post-placebo post-drug withered heart rate;
PT-post-drug withered heart rate in drug treatment zone.
The result of the calculation retains two decimal places.
The test results are shown in Table 7.
TABLE 7 field control of Chilo suppressalis by mixing Ditrifluouron with spinosad
As can be seen from the field efficacy test in Table 7, the mixed pesticide composition of the bistrifluron and spinosad has good control effect on chilo suppressalis. The control effect of 30% of the water dispersible granule of the bistrifluron and spinosad (1:2), 20% of the water dispersible granule of the bistrifluron and spinosad (1:3) and 28% of the suspending agent of the bistrifluron and spinosad (2:5) on the chilo suppressalis is higher than 83%.
Field test 3 field control test crop of greenhouse trialeurodes vaporariorum, dinotefuran, flonicamid and nitenpyram mixed: cucumber;
test target: white fly in greenhouse;
test site: the test is carried out in a vegetable base greenhouse in Weifang, shandong province; and planting in the period of 3 months and 15 days in 2020, wherein the cucumber variety is jin you.
The test land is loam, and the organic matter content is 1.2% -1.3%. The planting density is 3500-3700 plants/mu, the cucumber growth vigor is consistent, and the management is good.
The test method comprises the following steps: the test adopts a foliar spray method, and the test agent is uniformly sprayed on the front and back surfaces of the leaves. Area per cell 20m 2 Each treatment was repeated 4 times.
Investigation time and number: investigation time test the number of insects was investigated 3 times by spraying the drug on 5 months and 12 days of 2020, investigating the number of insects before the drug was applied, and investigating the number of living insects 3d and 10d after the drug was applied.
The investigation method comprises the following steps: 5 points are sampled in each cell, 5 cucumber plants are fixed in each cell, 2 leaves at the middle and upper parts of the cucumber plants are marked in a hanging mode, 25 plants are investigated in each cell, and the root numbers of the greenhouse powdery mildew insect mouths and the number of residual living insects on the fixed plants are respectively investigated.
The drug effect calculation method comprises the following steps:
the result of the calculation retains two decimal places.
The test results are shown in Table 8.
Table 8 field control effects of mixing bistrifluron with dinotefuran, flonicamid and nitenpyram on greenhouse trialeurodes vaporariorum
As shown in the field efficacy test of Table 8, the control effect of the mixed pesticide composition of the bistrifluron and dinotefuran and flonicamid and nitenpyram on the greenhouse powdery mildew is better than that of a control single agent.
In conclusion, through indoor tests and field efficacy tests, the insecticidal composition containing the bistrifluron has good control effects on various agricultural, forestry and gardening pests. Compared with a single dose, the insecticidal composition reduces the dosage, reduces the residual quantity of pesticides on crops and reduces the environmental pollution.

Claims (10)

1. The insecticidal composition containing the bistrifluron and the application thereof are characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is bistrifluron, the active ingredient B is nitenpyram, and the mass ratio of the active ingredient A to the active ingredient B is 1:30-25:1.
2. The insecticidal composition according to claim 1, wherein the mass ratio of the bistrifluron to the nitenpyram is 1:10-7:1.
3. A pesticidal composition according to claim 1, wherein the content of active ingredient a and active ingredient B in the pesticidal composition is 1 to 80wt%, preferably 2 to 60wt%.
4. The insecticidal composition according to claim 1, wherein the insecticidal composition comprises, in addition to the active ingredient, an adjuvant selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, stabilizers, penetrants and carriers.
5. The insecticidal composition of claim 4, wherein said insecticidal composition is formulated in any agriculturally acceptable formulation.
6. The insecticidal composition according to claim 5 wherein said formulation is selected from any one of powders, granules, soluble powders, soluble granules, soluble tablets, water dispersible granules, wettable powders, water dispersible tablets, dispersible liquids, emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and soluble solutions;
Preferably, the preparation formulation is selected from any one of suspending agents, emulsifiable concentrates, water dispersible granules and dispersible oil suspending agents.
7. Use of the insecticidal composition according to any one of claims 1 to 6 for controlling agricultural, forestry, horticultural pests.
8. The use according to claim 7, wherein the pest is a lepidopteran or hemipteran pest.
9. The use according to claim 8, wherein the lepidopteran pest comprises plutella xylostella, spodoptera exigua, prodenia litura, chilo suppressalis, armyworm, cotton bollworm, cabbage caterpillar; the hemipteran pests comprise whiteflies, rice planthoppers and aphids.
10. The use according to claim 7, wherein the pesticidal composition is applied to the pest or growth medium thereof in need of control in an effective amount.
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WO2007112843A1 (en) * 2006-03-29 2007-10-11 Bayer Cropscience Ag Active ingredient combinations with insecticidal properties
CN106665635A (en) * 2016-12-20 2017-05-17 天津市华宇农药有限公司 Insecticide composition containing acetamiprid and application thereof
WO2019027249A1 (en) * 2017-08-01 2019-02-07 주식회사 팜한농 Pest control composition including bistrifluron and indoxacarb as active ingredients
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