CN116640716A - Culture medium for increasing growth rate of lactobacillus plantarum and preparation method thereof - Google Patents
Culture medium for increasing growth rate of lactobacillus plantarum and preparation method thereof Download PDFInfo
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- CN116640716A CN116640716A CN202310702014.7A CN202310702014A CN116640716A CN 116640716 A CN116640716 A CN 116640716A CN 202310702014 A CN202310702014 A CN 202310702014A CN 116640716 A CN116640716 A CN 116640716A
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- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 61
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 61
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 61
- 239000001963 growth medium Substances 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title claims abstract description 6
- 244000068988 Glycine max Species 0.000 claims abstract description 55
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 55
- 239000005862 Whey Substances 0.000 claims abstract description 41
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- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims abstract description 15
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- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 102000015728 Mucins Human genes 0.000 description 1
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- 244000046052 Phaseolus vulgaris Species 0.000 description 1
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- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
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- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Abstract
The invention provides a culture medium for increasing the growth rate of lactobacillus plantarum and a preparation method thereof, wherein the formula is as follows: the concentrated Huang Douqing-1100 parts, the glucose addition amount is 6 parts, the yeast extract powder addition amount is 5 parts, the sodium acetate addition amount is 5 parts, the ammonium citrate addition amount is 2 parts, the dipotassium hydrogen phosphate addition amount is 2 parts, the manganese sulfate addition amount is 0.05 part, and the tween addition amount is 80 parts. The method comprises the following steps: collecting soybean serum and filtering to obtain filtrate; heating and filtering the filtrate to remove denatured precipitate; adding a defoaming agent, and concentrating to obtain concentrated soybean whey; sequentially adding substances in the formula, and adjusting the pH value to obtain a liquid culture medium; sealing the liquid culture medium, and sterilizing to obtain the culture medium product. The invention takes the soybean whey as a main body to prepare the culture medium, so as to maximize the utilization rate of the soybean whey, not only realize the conversion of soybean byproducts into valuable substances and high added value, but also meet the demands of the market on nutrition, health, green and low-cost products.
Description
Technical Field
The invention relates to the technical field of soybean byproduct processing, in particular to a culture medium for increasing the growth rate of lactobacillus plantarum and a preparation method thereof.
Background
Probiotic refers to an active microorganism that produces a health benefit to the host when ingested in sufficient quantity. Probiotics have functions of regulating intestinal flora, maintaining intestinal mucosa barrier, resisting bacteria, regulating immunity and the like, and have been widely used in various fields such as food processing, human health, livestock breeding and the like. Among them, lactic acid bacteria are a large class of probiotics and play a very important role in the human body. Lactic acid bacteria have the ability to lower the pH of the growing environment and can produce antibacterial agents that have many benefits to human health, such as: lactobacillus plantarum can survive in the gastrointestinal tract and colonize the intestinal tract, and is safe for human body. After ingestion of lactobacillus plantarum, the amount of enterobacteria carried in the feces is reduced, the level of causative agents of coronary heart disease is reduced, and the symptoms of IBS are alleviated. Meanwhile, lactobacillus plantarum secretes bioactive molecules to excite immune response, and can be used as a live vaccine to provide wider treatment in medicine. Competitive adhesion of lactobacillus plantarum to mannose-specific receptors, induction of human mucin gene expression to reduce pathogenicity, and the like. Thus, a nutrient medium and a suitable bacterial growth environment are required for the cultivation of probiotics, and nutrients required for the growth of lactic acid bacteria include non-metals such as carbon, nitrogen, sulfur and phosphorusMetal elements such as mass, calcium, zinc, sodium, potassium, copper, manganese, magnesium, iron and the like, vitamins, water and energy. The bean curd waste water, i.e. soybean whey, is a by-product produced in the bean curd processing process, and is generally discharged at about 30-50m per 1 ton of soybean 3 Waste water, including about 1 ton of waste water from bean soaking, 4-5 tons of soybean milk water, and 10 tons of clean waste water. Wherein, the soybean whey contains a large amount of water-soluble nutritional functional substances derived from soybean, which not only contains high content of proteins, oligosaccharides and isoflavones, but also contains rich nutrients such as calcium, phosphorus, potassium, iron, monosaccharides, B vitamins, amino acids, organic acids, etc. Therefore, the soybean whey can meet most of substances for the growth of probiotics, can provide unnecessary active substances, is beneficial to the growth and cultivation of the probiotics, and can greatly reduce the microbial cultivation cost by taking the soybean whey as a substrate for cultivating the microorganisms.
At present, a great difficulty still exists in how to comprehensively utilize soybean byproducts to realize the development of higher quality, and the soybean byproducts are mainly used for extracting functional components, fermenting or discarding. And many researches are developed in the food research field for the recycling problem of soybean byproducts. Wang Qian and the like, soybean wastewater is used as a raw material to cultivate the pleurotus nebrodensis, the period for preparing the pleurotus nebrodensis liquid strain is short, the activity is strong, and the optimal culture conditions are that the initial pH is 6.5, the inoculum size is 10% and the rotating speed of a shaking table is 180r/min. The nutritional value of the cordyceps militaris mycelium cultured by artificial liquid is similar to that of natural cordyceps militaris, wang Lixia is selected to inoculate cordyceps militaris SN-18 in yellow serofluid, the biomass of the mycelium is gradually increased to 13.01g/100mL, the mycelium tends to be stable from the 6 th day, and the functional activity of the yellow serofluid is also improved in the fermentation process.
According to the invention, the soybean whey is taken as a main body, and a proper amount of nutrient elements are added to supplement nutrition to culture probiotics, especially lactobacillus plantarum, so that a novel lactobacillus plantarum industrial culture medium which is low in price, large-scale in application and environment-friendly is developed, the wastewater treatment cost of enterprises is reduced, the expenditure is saved, the ecological environment is protected, the additional economic value of the soybean whey is improved, the profit is improved, and the main stream of the current society is met with the aim of green sustainable development.
Disclosure of Invention
The technical problems to be solved are as follows: the invention aims to provide a culture medium for increasing the growth rate of lactobacillus plantarum and a preparation method thereof, which take soybean whey as a main body, realize the maximization of the utilization rate of the soybean whey, realize the conversion of soybean byproducts into valuable substances and high added value, and meet the demands of the market on nutrition, health, green and low-cost products.
The technical scheme is as follows: a culture medium for increasing the growth rate of lactobacillus plantarum, wherein the culture medium comprises the following formula: the concentrated Huang Douqing-1100 parts, the glucose addition amount is 6 parts, the yeast extract powder addition amount is 5 parts, the sodium acetate addition amount is 5 parts, the ammonium citrate addition amount is 2 parts, the dipotassium hydrogen phosphate addition amount is 2 parts, the manganese sulfate addition amount is 0.05 part, and the tween addition amount is 80 parts.
A method of preparing a medium for increasing the growth rate of lactobacillus plantarum, the method comprising the steps of:
(1) Collecting bean curd processing byproducts of soybean whey;
(2) Filtering the soybean whey in the step (1) to obtain filtrate;
(3) Heating and filtering the filtrate obtained in the step (2) to remove denatured precipitate, and obtaining filtrate again;
(4) Adding 0.1% of defoaming agent into the filtrate obtained in the step (3), concentrating by using a rotary evaporator until the total volume is 1/3, and filtering to obtain concentrate Huang Douqing;
(5) Sequentially adding glucose, yeast extract powder, sodium acetate, ammonium citrate, dipotassium hydrogen phosphate, manganese sulfate and tween, shaking and mixing uniformly, and regulating pH to 6.3-6.5 to obtain a liquid culture medium;
(6) Sealing and sterilizing the liquid culture medium in the step (5) to obtain a culture medium finished product capable of increasing the growth rate of lactobacillus plantarum. Further, the filtering in the step (2) and the step (3) uses a 200-mesh filter screen.
Further, the heating conditions in the step (3) are as follows: under normal pressure, water bath is carried out at 70-90 ℃ for 8-12min.
Further, the conditions of the rotary evaporator in the step (4) are as follows: the vacuum degree of one standard atmosphere is 60-65 deg.C, and the rotation speed is 20rpm.
Further, the reagent for adjusting the pH in the step (5) is hydrochloric acid solution or sodium hydroxide solution, and the concentration of the reagent and the solution is 0.1mol/L.
Further, in the step (6), specific conditions for sterilization are: sterilizing at 120deg.C for 15-20min. The beneficial effects are that: the invention has the following advantages:
1. the invention takes the soybean whey as a main body, and the soybean whey contains abundant water-soluble nutritional functional substances, including proteins, oligosaccharides and isoflavone, and also contains nutritional substances such as calcium, phosphorus, potassium, iron, monosaccharide, B vitamins, amino acids, organic acids and the like. Lactobacillus plantarum lacks an enzyme system that breaks down macromolecular carbohydrates, so some monosaccharides are often used to maintain its own growth; the monosaccharide does not need to be decomposed, so that the energy consumption of thalli is reduced, and the growth and the reproduction of lactobacillus plantarum are further promoted; the soybean whey contains functional soybean oligosaccharides, which are composed of monosaccharide molecules, and can promote the absorption of nutrients of lactobacillus plantarum, for example: stachyose is a member of stachyose in soybean whey, is a prebiotic in nature, and can induce synthesis of mitogen and promote proliferation of probiotics. Meanwhile, the lactobacillus plantarum has low decomposition and utilization rate of inorganic nitrogen, so that only a few small molecules rich in amino acids, peptides and the like can be relied on to provide nitrogen sources, and the nitrogen sources such as polypeptide, amino acids and the like contained in the soybean whey can promote the synthesis of cell walls (proteins, amino acids) and metabolites of lactobacillus plantarum. Therefore, the cooperation of functional substances such as oligosaccharide, polypeptide and the like in the soybean whey promotes the reproduction and growth of lactobacillus plantarum, wherein the oligosaccharide can also play a role in stabilizing the culture medium. The culture medium can furthest utilize nutrient elements contained in the soybean whey, reduce the supplement of external nutrition, meet most of substances required by the growth of probiotics, provide unnecessary active substances, be beneficial to the growth and cultivation of the probiotics, remarkably improve the growth rate of the cultured probiotics and shorten the cultivation time.
2. The invention has simple process and low production cost, is suitable for industrial production, solves the problem of recycling soybean byproducts for soybean processing enterprises, reduces the production cost, avoids the problem of environmental pollution, and greatly improves the added value of the soybean byproducts.
Drawings
FIG. 1 is a graph showing growth curves and growth rate constants of Lactobacillus plantarum of different strain models in examples and comparative examples, wherein (a) a Lactobacillus plantarum of HCS03-084 model, (b) a Lactobacillus plantarum of ST-III model, (c) a Lactobacillus plantarum of JYLP-326 model, (d) a Lactobacillus plantarum of JYLP-002 model, (e) a Lactobacillus plantarum of HH-LP56 model, and (f) a Lactobacillus plantarum of different strain models are shown in examples and comparative examples;
FIG. 2 shows the total number of colonies of Lactobacillus plantarum cultured in examples and comparative examples;
fig. 3 is a finished product diagram of example 1, example 2, example 3, example 4 and comparative example 1.
Detailed Description
The invention will be further described with reference to the accompanying drawings and examples, which are illustrative of the invention and not intended to limit the invention to the examples below:
example 1
A culture medium for increasing the growth rate of lactobacillus plantarum comprises the following formula: 900mL of concentrated soybean whey, 5.4g of glucose, 4.5g of yeast extract powder, 4.5g of sodium acetate, 1.8g of ammonium citrate, 1.8g of dipotassium hydrogen phosphate, 0.045g of manganese sulfate and 72g of tween;
the method comprises the following steps:
(1) Collecting bean curd processing byproducts of soybean whey;
(2) Filtering the soybean whey in the step (1) by using a 200-mesh filter screen to obtain filtrate;
(3) Heating the filtrate obtained in the step (2) under normal pressure, carrying out water bath at 70 ℃ for 8min, filtering, removing denatured precipitate, and obtaining filtrate again;
(4) Adding 0.1% defoaming agent into the filtrate obtained in the step (3), setting the condition of a rotary evaporator to be the vacuum degree of standard atmospheric pressure, setting the temperature to be 60 ℃, and concentrating by using the rotary evaporator at the rotating speed of 20rpm until the total volume is 1/3, and filtering to obtain concentrate Huang Douqing;
(5) Glucose, yeast extract powder, sodium acetate, ammonium citrate, dipotassium hydrogen phosphate, manganese sulfate and tween are sequentially added according to the proportion, the mixture is uniformly mixed by shaking, and the pH value is regulated to 6.3 by using a hydrochloric acid solution with the concentration of 0.1mol/L to obtain a liquid culture medium;
(6) Sealing the liquid culture medium in the step (5), sterilizing at the ultra-high temperature for a long time, and sterilizing at 120 ℃ for 15min to obtain a culture medium finished product capable of increasing the growth rate of lactobacillus plantarum.
Example 2
A culture medium for increasing the growth rate of lactobacillus plantarum comprises the following formula: 950mL of concentrated soybean whey, 5.7g of glucose, 4.75g of yeast extract powder, 4.75g of sodium acetate, 1.9g of ammonium citrate, 1.9g of dipotassium hydrogen phosphate, 0.048g of manganese sulfate and 76g of tween;
the method comprises the following steps:
(1) Collecting bean curd processing byproducts of soybean whey;
(2) Filtering the soybean whey in the step (1) by using a 200-mesh filter screen to obtain filtrate;
(3) Heating the filtrate obtained in the step (2) under normal pressure, carrying out water bath at 80 ℃ for 10min, filtering, removing denatured precipitate, and obtaining filtrate again;
(4) Adding 0.1% defoaming agent into the filtrate obtained in the step (3), setting the condition of a rotary evaporator to be the vacuum degree of standard atmospheric pressure, setting the temperature to be 63 ℃, and concentrating by using the rotary evaporator at the rotating speed of 20rpm until the total volume is 1/3, and filtering to obtain concentrate Huang Douqing;
(5) Glucose, yeast extract powder, sodium acetate, ammonium citrate, dipotassium hydrogen phosphate, manganese sulfate and tween are sequentially added according to the proportion, the mixture is uniformly mixed by shaking, and the pH value is regulated to 6.3 by using a hydrochloric acid solution with the concentration of 0.1mol/L to obtain a liquid culture medium;
(6) Sealing the liquid culture medium in the step (5), sterilizing at the ultra-high temperature for a long time, and sterilizing at 120 ℃ for 18min to obtain a finished product of the culture medium capable of increasing the growth rate of lactobacillus plantarum.
Example 3
A culture medium for increasing the growth rate of lactobacillus plantarum comprises the following formula: 1000mL of concentrated soybean whey, 6g of glucose addition, 5g of yeast extract powder addition, 5g of sodium acetate addition, 2g of ammonium citrate addition, 2g of dipotassium hydrogen phosphate addition, 0.05g of manganese sulfate addition and 80g of tween addition;
the method comprises the following steps:
(1) Collecting bean curd processing byproducts of soybean whey;
(2) Filtering the soybean whey in the step (1) by using a 200-mesh filter screen to obtain filtrate;
(3) Heating the filtrate obtained in the step (2) under normal pressure, carrying out water bath at 90 ℃ for 10min, filtering, removing denatured precipitate, and obtaining filtrate again;
(4) Adding 0.1% defoaming agent into the filtrate obtained in the step (3), setting the condition of a rotary evaporator to be the vacuum degree of standard atmospheric pressure, setting the temperature to be 65 ℃, and concentrating by using the rotary evaporator at the rotating speed of 20rpm until the total volume is 1/3, and filtering to obtain concentrate Huang Douqing;
(5) Sequentially adding glucose, yeast extract powder, sodium acetate, ammonium citrate, dipotassium hydrogen phosphate, manganese sulfate and tween into the concentrated solution in the step (4) according to the proportion, shaking and mixing uniformly, and adjusting the pH value to 6.5 by using a sodium hydroxide solution with the concentration of 0.1mol/L to obtain a liquid culture medium;
(6) Sealing the liquid culture medium in the step (5), sterilizing at the ultra-high temperature for a long time, and sterilizing at 120 ℃ for 20min to obtain a culture medium finished product capable of increasing the growth rate of lactobacillus plantarum.
Example 4
A culture medium for increasing the growth rate of lactobacillus plantarum comprises the following formula: 1100mL of concentrated soybean whey, 6.6g of glucose, 5.5g of yeast extract powder, 5.5g of sodium acetate, 2.2g of ammonium citrate, 2.2g of dipotassium hydrogen phosphate, 0.055g of manganese sulfate and 88g of tween;
the method comprises the following steps:
(1) Collecting bean curd processing byproducts of soybean whey;
(2) Filtering the soybean whey in the step (1) by using a 200-mesh filter screen to obtain filtrate;
(3) Heating the filtrate obtained in the step (2) under normal pressure, carrying out water bath at 90 ℃ for 12min, filtering, removing denatured precipitate, and obtaining filtrate again;
(4) Adding 0.1% defoaming agent into the filtrate obtained in the step (3), setting the condition of a rotary evaporator to be the vacuum degree of standard atmospheric pressure, setting the temperature to be 65 ℃, and concentrating by using the rotary evaporator at the rotating speed of 20rpm until the total volume is 1/3, and filtering to obtain concentrate Huang Douqing;
(5) Sequentially adding glucose, yeast extract powder, sodium acetate, ammonium citrate, dipotassium hydrogen phosphate, manganese sulfate and tween into the concentrated solution in the step (4) according to the proportion, shaking and mixing uniformly, and adjusting the pH value to 6.5 by using a sodium hydroxide solution with the concentration of 0.1mol/L to obtain a liquid culture medium;
(6) Sealing the liquid culture medium in the step (5), sterilizing at the ultra-high temperature for a long time, and sterilizing at 120 ℃ for 20min to obtain a culture medium finished product capable of increasing the growth rate of lactobacillus plantarum.
Comparative example 1
The comparative example differs from example 3 in that the whey used was unconcentrated whey, specifically:
a culture medium for increasing the growth rate of lactobacillus plantarum comprises the following formula: 1000mL of concentrated soybean whey, 6g of glucose addition, 5g of yeast extract powder addition, 5g of sodium acetate addition, 2g of ammonium citrate addition, 2g of dipotassium hydrogen phosphate addition, 0.05g of manganese sulfate addition and 80g of tween addition;
the method comprises the following steps:
(1) Collecting bean curd processing byproducts of soybean whey;
(2) Filtering the soybean whey in the step (1) by using a 200-mesh filter screen to obtain filtrate;
(3) Sequentially adding glucose, yeast extract powder, sodium acetate, ammonium citrate, dipotassium hydrogen phosphate, manganese sulfate and tween into the filtrate in the step (2) according to the proportion, shaking and mixing uniformly, and adjusting the pH value to 6.5 by using a sodium hydroxide solution with the concentration of 0.1mol/L to obtain a liquid culture medium;
(4) Sealing the liquid culture medium in the step (3), sterilizing at the ultra-high temperature for a long time, and sterilizing at 120 ℃ for 20min to obtain a culture medium finished product capable of increasing the growth rate of lactobacillus plantarum.
Performance testing of the media prepared in examples 1-4 with comparative example 1 and common MRS media (Beijing Obocin Biotechnology Co., ltd. 02-291) (comparative example 2)
1. Test index
1.1 Lactobacillus plantarum growth Curve detection
The growth conditions of lactobacillus plantarum of different strain types in different culture media are detected by using a growth curve instrument, the wavelength is 600nm, the growth temperature is 37 ℃, the growth time is 24 hours, the growth rate is analyzed, and each group is subjected to 3 parallel tests.
1.2 detection of Lactobacillus plantarum colony count
The total number of Lactobacillus plantarum colonies was detected according to the method of GB 4789.2-2016.
2. Experimental results
2.1 Lactobacillus plantarum growth Curve
As shown in FIG. 1, the growth curves and growth rate constants of Lactobacillus plantarum of 5 different strain models were cultivated using the media prepared in examples 1-4 and comparative examples 1-2, respectively. Overall, the growth rate of example 3 was significantly better than examples 1, 2 and 4; meanwhile, for most lactobacillus plantarum, the growth rate of example 3 was increased by 9% to 38% compared to comparative example 1, and the growth rate was increased by 16% to 86% compared to comparative example 2. This is because lactobacillus plantarum lacks an enzyme system that breaks down macromolecular carbohydrates, so some monosaccharides are often used to maintain its own growth; the monosaccharide does not need to be decomposed, so that the energy consumption of thalli is reduced, and the growth and the reproduction of lactobacillus plantarum are further promoted; the soybean whey contains rich functional soybean oligosaccharides which are composed of monosaccharide molecules, so that the nutrient absorption of lactobacillus plantarum can be promoted.
2.2 total number of Lactobacillus plantarum colonies
As shown in FIG. 2, the total number of colonies of different Lactobacillus plantarum were cultivated in examples and comparative examples, respectively, to illustrate the cultivation and proliferation effects of the medium on Lactobacillus plantarum. Overall, the total number of colonies of example 3 was greater than that of examples 1, 2, and 4; meanwhile, the culture effect of example 3 on lactobacillus plantarum was increased by 62% to 102% as compared with comparative example 1, and by 14% to 183% as compared with comparative example 2. This is because the soybean whey contains abundant growth factors such as: organic acid, amino acid, purine, vitamin, mineral elements and the like can meet the requirement of growth factors under the condition that a lactobacillus plantarum physiological system lacks the capability of synthesizing various compounds, and promote the growth and the reproduction of thalli.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (7)
1. A culture medium for increasing the growth rate of lactobacillus plantarum, characterized in that: the formula of the culture medium is as follows: the concentrated Huang Douqing-1100 parts, the glucose addition amount is 6 parts, the yeast extract powder addition amount is 5 parts, the sodium acetate addition amount is 5 parts, the ammonium citrate addition amount is 2 parts, the dipotassium hydrogen phosphate addition amount is 2 parts, the manganese sulfate addition amount is 0.05 part, and the tween addition amount is 80 parts.
2. A preparation method of a culture medium for increasing the growth rate of lactobacillus plantarum is characterized by comprising the following steps: the method comprises the following steps:
(1) Collecting bean curd processing byproducts of soybean whey;
(2) Filtering the soybean whey in the step (1) to obtain filtrate;
(3) Heating and filtering the filtrate obtained in the step (2) to remove denatured precipitate, and obtaining filtrate again;
(4) Adding 0.1% of defoaming agent into the filtrate obtained in the step (3), concentrating by using a rotary evaporator until the total volume is 1/3, and filtering to obtain concentrate Huang Douqing;
(5) Sequentially adding glucose, yeast extract powder, sodium acetate, ammonium citrate, dipotassium hydrogen phosphate, manganese sulfate and tween, shaking and mixing uniformly, and regulating pH to 6.3-6.5 to obtain a liquid culture medium;
(6) Sealing and sterilizing the liquid culture medium in the step (5) to obtain a culture medium finished product capable of increasing the growth rate of lactobacillus plantarum.
3. The method for preparing the culture medium for increasing the growth rate of lactobacillus plantarum according to claim 1, wherein the method comprises the following steps: and filtering in the step (2) and the step (3) by using a 200-mesh filter screen.
4. The method for preparing the culture medium for increasing the growth rate of lactobacillus plantarum according to claim 1, wherein the method comprises the following steps: the heating conditions in the step (3) are as follows: under normal pressure, water bath is carried out at 70-90 ℃ for 8-12min.
5. The method for preparing the culture medium for increasing the growth rate of lactobacillus plantarum according to claim 1, wherein the method comprises the following steps: the conditions of the rotary evaporator in the step (4) are as follows: the vacuum degree of one standard atmosphere is 60-65 deg.C, and the rotation speed is 20rpm.
6. The method for preparing the culture medium for increasing the growth rate of lactobacillus plantarum according to claim 1, wherein the method comprises the following steps: the reagent for regulating the pH in the step (5) is hydrochloric acid solution or sodium hydroxide solution, and the concentration of the reagent and the solution is 0.1mol/L.
7. The method for preparing the culture medium for increasing the growth rate of lactobacillus plantarum according to claim 1, wherein the method comprises the following steps: in the step (6), the specific conditions for sterilization are: sterilizing at 120deg.C for 15-20min.
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