Summary of the invention:
Main purpose of the present invention is to find the economic and practical method that can improve oophagous trichogrammae pest control effect.
(is example with trichogramma dendrolimi control of maize snout moth's larva) is present, and limit cheaply because of accepting a surrender the corn main producing region northeastward, and it is 22.5 ten thousand of per hectares that the production unit acceptable is put the honeybee amount, puts the honeybee secondary every year.Control efficiency only has about 65%.
The inventor thinks that oophagous trichogrammae can seek insect ovum parasitism voluntarily, can breed in the field, and non-resistant, no residual hazard if find way simple and easy to do, that the market acceptable improves oophagous trichogrammae product control efficiency, can enlarge the oophagous trichogrammae popularizing area.
But the present invention utilizes the characteristics of oophagous trichogrammae honeybee body load microorganism, make oophagous trichogrammae carry bacterium for killing insect in artificial mode, make product dispensing field, oophagous trichogrammae spreads to the host egg surface with bacterium for killing insect in seeking the process of host egg, has honeybee the go out double effects of larva of ovum, bacterium that goes out.
The pieces of an egg that every bacteria-carrying trichogramma was sought all have bacterium for killing insect to be lost in the ovum face.The larva of (corn borer etc.) insect has the habit of eating out earlier chorion when hatching, and larva is infected.It is dead than very fast that larva infects the back, and only some reached for three ages, and insect was killed before the gluttony phase.The level that this method has reached the use of bacteria preparation and saves the most, usefulness is the highest.Also finding in field trial has predators such as the worm of wafing, Chrysopa to crawl to this product, also can carry bacterium and continue to propagate, and plays the common effect of scattering of common breeding in the field of oophagous trichogrammae and pathogenic bacteria.
Abroad have with the swollen leg honeybee of cotton and carry the report of prevention and cure of viruses cotton bollworm, there was the report that carries the anti-happy cotton bollworm of virus with trichogramma dendrolimi in China in 1997, still was difficult the report that oophagous trichogrammae is carried the bacterium pest control so far both at home and abroad.The present invention is a breakthrough of domestic and international biological and ecological methods to prevent plant disease, pests, and erosion technology, and oophagous trichogrammae series of products control efficiency is compiled low, and intuitive is relatively poor, has influenced the promotion rate of this kind technology.Compared with prior art, have the following advantages:
1, compare with independent trichogramma releases, the present invention has increased the function of killing larva, and control efficiency greatly improves, and the control efficiency that reduces corn borer worm amount alive in autumn through field trial reaches 87.2%.Among the whole bag of tricks of control of maize snout moth's larva, belong to the highest class of preventive effect.
2, the effect of bacteria preparation granule control of maize snout moth's larva can reach about 85%, but takes a lot of work because of use, needs artificially to the corn lobus cardiacus to be the difficulty acceptance of user institute by spreading pesticides.Compare with bacteria preparation, easy to use, practical, this product can be understood as a kind of application process of desirable bacteria preparation.The a spot of bacteria preparation of this product oophagous trichogrammae load is lost in insect ovum face with bacterium when seeking host egg, the direct infection larva, and natural bacteria using amount is little, the preventive effect height.
With oophagous trichogrammae in spite of illness poison compare and have his own strong points, viral product control efficiency height, special main parasitic habit is strong, so during breeding than bacterium difficulty, especially insect such as corn borer is not also found Causative virus.
From economic benefit, this product control cost is compared with the oophagous trichogrammae product and is increased seldom, and control increases much than fruit.
Host, honeybee kind, bacterial classification, controlling object, the dispensing mode difference of breeding oophagous trichogrammae realize the best way of invention: because of can be made multiple product.The mode that realizes invention also is diversified.As: current control of maize snout moth's larva, can take following several prods:
1, with egg of oak silkworm, artificial propagation trichogramma dendrolimi, the yellow oophagous trichogrammae of maggot, trichogramma evanescens mix in 8: 1: 1 ratios;
2, manually expand the pathogenic bacteria of corn borers such as numerous bacillus thuringiensis,Bt, white muscardine fungi, green muscardine fungus;
3, make the honeybee card, bee-escape carries disease germs honeybee or makes the long-acting honeybee card of different development stage.
But above-mentioned formed product control of maize snout moth's larva, cabbage army worm, imported cabbageworm, soybean pod borer, jowar bar snout moth's larva, pine caterpillars, slug moth or the like.
Used honeybee kind, bacterial classification extensively exist at natural world, can go screening from the honeybee kind that collects, bacterial classification, purify, expand numerous.