CN116617238A - Application of Chinese herbal medicine saffron glycoside I in fish antiviral - Google Patents
Application of Chinese herbal medicine saffron glycoside I in fish antiviral Download PDFInfo
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- CN116617238A CN116617238A CN202310618446.XA CN202310618446A CN116617238A CN 116617238 A CN116617238 A CN 116617238A CN 202310618446 A CN202310618446 A CN 202310618446A CN 116617238 A CN116617238 A CN 116617238A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 18
- 229930182470 glycoside Natural products 0.000 title claims description 10
- 150000002338 glycosides Chemical class 0.000 title claims description 10
- 244000124209 Crocus sativus Species 0.000 title claims description 7
- 235000015655 Crocus sativus Nutrition 0.000 title claims description 7
- 235000013974 saffron Nutrition 0.000 title claims description 7
- 239000004248 saffron Substances 0.000 title claims description 7
- 230000000840 anti-viral effect Effects 0.000 title abstract description 11
- 241000411851 herbal medicine Species 0.000 title description 8
- SEBIKDIMAPSUBY-RTJKDTQDSA-N crocin-1 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O1)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SEBIKDIMAPSUBY-RTJKDTQDSA-N 0.000 claims abstract description 48
- 241000700605 Viruses Species 0.000 claims abstract description 23
- 239000003814 drug Substances 0.000 claims abstract description 8
- 230000009385 viral infection Effects 0.000 claims abstract description 7
- 241000036569 Carp sprivivirus Species 0.000 claims abstract description 6
- 241000252212 Danio rerio Species 0.000 abstract description 54
- 241001662595 Gardneria Species 0.000 abstract description 12
- 230000000366 juvenile effect Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 6
- 230000010076 replication Effects 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000003612 virological effect Effects 0.000 abstract description 2
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 24
- SEBIKDIMAPSUBY-ARYZWOCPSA-N Crocin Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)C(C)=CC=CC(C)=C\C=C\C=C(/C)\C=C\C=C(C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O1)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SEBIKDIMAPSUBY-ARYZWOCPSA-N 0.000 description 9
- SEBIKDIMAPSUBY-JAUCNNNOSA-N Crocin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C(=O)OC1OC(COC2OC(CO)C(O)C(O)C2O)C(O)C(O)C1O)C=CC=C(/C)C(=O)OC3OC(COC4OC(CO)C(O)C(O)C4O)C(O)C(O)C3O SEBIKDIMAPSUBY-JAUCNNNOSA-N 0.000 description 9
- 239000011550 stock solution Substances 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 8
- 206010058874 Viraemia Diseases 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 241000252233 Cyprinus carpio Species 0.000 description 5
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- 230000013011 mating Effects 0.000 description 4
- 238000003753 real-time PCR Methods 0.000 description 4
- 231100000419 toxicity Toxicity 0.000 description 4
- 230000001988 toxicity Effects 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000344 non-irritating Toxicity 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000448255 Congiopodus peruvianus Species 0.000 description 1
- 241000252230 Ctenopharyngodon idella Species 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001275898 Mylopharyngodon piceus Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000711931 Rhabdoviridae Species 0.000 description 1
- 108091005770 SIRT3 Proteins 0.000 description 1
- 102000000478 Sirtuin 3 Human genes 0.000 description 1
- 208000026137 Soft tissue injury Diseases 0.000 description 1
- 101001039859 Spring viremia of carp virus Matrix protein Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000008845 cholagoga Substances 0.000 description 1
- 229940124571 cholagogue Drugs 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229940031551 inactivated vaccine Drugs 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108010008359 protein kinase C lambda Proteins 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7024—Esters of saccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses an application of crocin I (Gardneria yellow) in preparing medicaments for preventing or treating fish virus infection, which is discovered by carrying out a virus attack experiment of SVCV virus on juvenile fish of zebra fish, the crocin I can obviously improve the survival rate of the zebra fish after the zebra fish is infected by the SVCV virus and strengthen antiviral genesifn1、mxc、pkzWhile significantly inhibiting the replication capacity of the viral SVCV. Therefore, the crocin I can be used for preparing medicaments for preventing or treating fish antiviral (especially spring viremia of carp virus).
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to application of Chinese herbal medicine saffron glucoside I in fish antiviral.
Background
Spring viremia of carp (Spring viraemia of carp, SVC) is a viral disease of aquatic animals which seriously damages fishes of the carp family, and has the characteristics of high mortality rate, strong infectivity, acute morbidity and the like. The direct pathogen of the spring viremia of carp is spring viremia virus (Spring viraemia of carp virus, SVCV), the SVCV belongs to Rhabdoviridae virus, and can infect main species of freshwater aquaculture of Chinese black carp, grass carp and the like, which often causes a great deal of death of fish and causes huge economic loss.
At present, the prevention and treatment method for fish virus diseases in China mainly comprises the following steps: adding immune preparation, improving culture mode, preventing and controlling inactivated vaccine, preventing and controlling with external medicine, etc. However, the method lacks specificity, has insignificant effects and obvious side effects, is easy to cause drug resistance of fishes, can pollute the surrounding water environment, and is very unfavorable for the development of aquaculture industry. However, the Chinese herbal medicine is extracted as the traditional medicine in China, the probability of causing pathogen variation is low, the drug resistance is not easy to form, the application cost is low, the ecological friendliness is high, the double-effect function of individual and group control is realized, and the healthy sustainable development of aquaculture is realized.
Saffron glycoside I (Gardneria yellow) is a member of the crocin family, and is one of the main active ingredients of saffron. The study shows that the crocin I can enhance the mRNA level of the deacetylase SIRT3, has pharmacological effects of central blood pressure reduction, atherosclerosis prevention, thrombus resistance, platelet aggregation resistance, myocardial ischemia resistance, inflammation resistance, soft tissue injury treatment, cholagogue liver protection, tumor resistance and the like, has wide application in industries such as food, medicine, chemical industry and the like, and is clinically applied to treatment of cardiovascular diseases and fatty liver patients. However, no related report exists on the study of the safflor glycoside I in antiviral innate immunity.
Disclosure of Invention
The invention aims to provide an application of Chinese herbal medicine crocin I (Gardneria yellow) in preparing a medicine or a feed additive for preventing or treating fish spring viremia virus infection.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
according to the invention, through zebra fish in-vivo experiments, when the content of the crocin I is 12 mug/ml, 24 mug/ml and 48 mug/ml, the zebra fish has no obvious toxicity to the juvenile fish after 48 hours of action; when the content of the crocin I is 24 mug/ml and 48 mug/ml, the crocin I can effectively improve the survival rate of the zebra fish after SVCV infection; at a crocin I content of 48 mug/ml, crocin I can significantly enhance the expression of zebra fish antiviral related genes (ifn, mxc, pkz) and significantly inhibit the replication capacity of SVCV virus. Therefore, the crocin I can be used for preparing medicines or feed additives for preventing or treating fish virus (especially spring viremia of carp virus).
Compared with the prior art, the invention has the beneficial effects that: the invention discovers and proves that the crocin I (Gardneria yellow) is a Chinese herbal medicine with the SVCV virus activity for resisting the spring viremia virus of carp for the first time, and the crocin I has the characteristic of resisting the fish virus through the toxicity attack experiment of SVCV virus on juvenile fish of zebra fish, so that the crocin I can be used for preparing antiviral drugs.
Drawings
FIG. 1 shows the effect of varying levels of crocin I (Gardneria yellow) on zebra fish toxicity.
FIG. 2 shows that crocin I (Gardneria yellow) enhances antiviral ability of zebra fish.
FIG. 3 is a graph showing the effect of crocin I (Gardneria yellow) on the survival of zebra fish after SVCV infection.
FIG. 4 shows that crocin I (Gardneria yellow) enhances the expression of the SVCV post-infection zebrafish antiviral related genes (ifn, mxc, pkz).
FIG. 5 shows that crocin I (Gardneria yellow) inhibits SVCV virus replication.
Detailed Description
The materials and reagents referred to in the following examples, the experimental conditions and methods are as follows:
1. materials and reagents
Materials: crocin I Gardneria yellow (available from MCE, CAS number 94238-00-3), solvent DMSO (available from Selleck);
virus: spring viremia of carp virus SVCV;
zebra fish: the AB strain zebra fish is from the national zebra fish resource center.
2. Method and procedure
Stock solution of crocin i (gardnenia yellow): 50mg of the commercially available safflor glycoside I powder was dissolved in 1.67ml of DMSO to prepare a 30mg/ml stock solution of safflor glycoside I.
Example 1 toxicity test of safflor glycoside I against Zebra fish
And (3) carrying out male-female mating on adult zebra fish of the AB strain to obtain a sufficient number of zebra fish larvae of three days old, randomly dividing the zebra fish larvae of three days old into 3 groups according to 30 groups, and culturing the zebra fish larvae by using 60mm culture dishes respectively. To each group of fish, 4ml of culture water was added, followed by 1ml of culture water containing 8. Mu.l of DMSO to a blank group (crocin I0. Mu.g/ml), and 1ml of culture water containing 2. Mu.l, 4. Mu.l, 8. Mu.l of crocin I stock solution was added to each group of test groups, at which time the crocin I content of the test groups was 12. Mu.g/ml, 24. Mu.g/ml, and 48. Mu.g/ml, respectively. The four groups of zebra fish juvenile fish are placed in a constant temperature incubator at 28 ℃, and after 48 hours, the state of the zebra fish is observed under a microscope and recorded by photographing, as shown in fig. 1.
According to the microscopic photographing result, the content of the Chinese herbal medicine crocin I (Gardneria yellow) is respectively 12 mug/ml, 24 mug/ml and 48 mug/ml, and the crocin I has no influence on the life activities and the living states of the juvenile zebra fish, so that the crocin I has no obvious toxicity on the juvenile zebra fish.
Example 2 safflor glycoside I enhances antiviral ability of zebra fish
And (3) carrying out male-female mating on adult zebra fish of the AB strain to obtain a sufficient number of zebra fish larvae of three days old, randomly dividing the zebra fish larvae of three days old into 6 groups according to 30 groups, and culturing the zebra fish larvae by using 60mm culture dishes. To the virus-free stimulated group, 4ml of culture water was added, respectively, followed by 1ml of culture water containing 8. Mu.l of DMSO to the blank group (crocin I0. Mu.g/ml), and 1ml of culture water containing 4. Mu.l and 8. Mu.l of crocin I stock solution were added to the experimental group crocin I24. Mu.g/ml group and the experimental group crocin I48. Mu.g/ml group, respectively. 2ml of culture water was added to the virus-stimulated group, 1ml of culture water containing 8. Mu.l of DMSO was added to the blank group (crocin I0. Mu.g/ml group), 1ml of culture water containing 4. Mu.l and 8. Mu.l of stock solution of crocin I was added to the experimental group crocin I24. Mu.g/ml group and the experimental group crocin I48. Mu.g/ml group, respectively, and then the three groups were stimulated with viruses2ml SVCV virus (. About.1.0X10) was added to each group 8 TCID 50 /ml). The 6 groups of young zebra fish are placed in a constant temperature incubator at 28 ℃, the states of the young zebra fish are observed every 4 hours, the death number is counted, and microscopic photographing records are carried out after virus infection for 40 hours, as shown in fig. 2, red arrows represent the dead young zebra fish. Zebra fish survival was counted using graphpad prism software and the results are shown in figure 3.
According to microscopic photographing results, the Chinese herbal medicine crocin I (Gardneria yellow) has the content of 24 mug/ml and 48 mug/ml, and the death number of the zebra fish in a blank control group is obviously more than that in an experimental group after SVCV virus infection for 40 hours, so that the crocin I can enhance the SVCV virus resistance of the zebra fish. Meanwhile, the death curve of FIG. 3 shows that the zebra fish in the control group is all dead after the SVCV virus infection for 44 hours, and the survival rate of the zebra fish treated by the crocin I content of 48 mug/ml is still more than 75%, which indicates that the crocin I can obviously improve the survival rate of the zebra fish after the SVCV virus infection.
EXAMPLE 3 saffron glycoside I enhances expression of the antiviral related Gene of Zebra fish
Mating the adult zebra fish of the AB strain to obtain a sufficient number of zebra fish larvae of three days old, randomly dividing the zebra fish larvae of three days old into 4 groups according to 30 groups, and culturing the zebra fish larvae by using a 60mm culture dish respectively. To two of the groups, 4ml of culture water was added, followed by 1ml of culture water containing 8. Mu.l of DMSO in one group and 1ml of culture water containing 8. Mu.l of stock solution of crocin I in the other group, at which time the crocin I content was 48. Mu.g/ml, both groups being non-irritating groups (i.e. -SVCV); 2ml of culture water was added to the other two groups, respectively, followed by 1ml of culture water containing 8. Mu.l of DMSO to the control group (crocin I), 1ml of culture water containing 8. Mu.l of crocin I stock solution was added to the experimental group crocin I, at which the crocin I content was 48. Mu.g/ml, and then 2ml of SVCV virus (. About.1.0X10) was added to each of the two groups 8 TCID 50 /ml), both groups are therefore stimulated groups (i.e. + SVCV). Culturing young zebra fish in a constant temperature incubator at 28deg.C for 24 hr, removing culture water, and collecting zebra fish in a 1.5ml centrifuge tubeThe total RNA of the zebra fish is extracted by a Trizol method, then cDNA is obtained by using a reverse transcription kit, real-time fluorescence quantitative PCR is carried out according to the requirements of SYBR Green qPCR mix specifications, the expression condition of the related genes (ifn, mxc, pkz) of the zebra fish is detected, and the sequences of the fluorescence quantitative PCR primers are shown in the table 1. The experimental results are shown in FIG. 4.
Experimental results show that the virus SVCV can obviously activate the expression of the related genes ifn1, mxc and pkz of the zebra fish virus, and meanwhile, the expression level of the related genes ifn1, mxc and pkz of the zebra fish virus can be obviously enhanced by the crocin I.
TABLE 1
EXAMPLE 4 the ability of crocin I to inhibit replication of SVCV Virus
Mating the adult zebra fish of the AB strain to obtain a sufficient number of zebra fish larvae of three days old, randomly dividing the zebra fish larvae of three days old into 4 groups according to 30 groups, and culturing the zebra fish larvae by using a 60mm culture dish respectively. To two of the groups, 4ml of culture water was added, followed by 1ml of culture water containing 8. Mu.l of DMSO in one group and 1ml of culture water containing 8. Mu.l of stock solution of crocin I in the other group, at which time the crocin I content was 48. Mu.g/ml, both groups being non-irritating groups (i.e. -SVCV); 2ml of culture water was added to the other two groups, respectively, followed by 1ml of culture water containing 8. Mu.l of DMSO to the control group (DMSO group), 1ml of culture water containing 8. Mu.l of stock solution of crocin I was added to the experimental group of crocin I, at which the crocin I content was 48. Mu.g/ml, and then 2ml of SVCV virus (. About.1.0X10) was added to the two groups, respectively 8 TCID 50 /ml), both groups are therefore stimulated groups (i.e. + SVCV). Culturing young zebra fish in a constant temperature incubator at 28deg.C for 24 hr, removing culture water, collecting zebra fish in a 1.5ml centrifuge tube, and treating spots by Trizol methodThe total RNA of the horse fish is extracted, cDNA is obtained by using a reverse transcription kit, real-time fluorescence quantitative PCR is carried out according to the requirements of SYBR Green qPCR mix specifications, and the expression of five genes (SVCV-P, SVCV-G, SVCV-N, SVCV-M and SVCV-L) of the SVCV virus is detected, wherein the sequences of the fluorescence quantitative PCR primers are shown in the table 1. The experimental results are shown in FIG. 5.
Experimental results show that the crocin I can obviously inhibit the expression of each component gene of the SVCV, and further shows that the crocin I can obviously inhibit the replication capacity of the SVCV.
Claims (3)
1. The application of crocin I in preparing medicine for preventing or treating fish virus infection is provided.
2. The use according to claim 1, wherein the virus is a spring viremia of carp virus.
3. Use according to claim 1, characterized in that saffron glycoside i is used in a concentration of 48 μg/ml.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019539A (en) * | 2007-02-27 | 2007-08-22 | 南开大学 | Interacting composition possessing activity of resisting tobacco mosaic virus |
| CN104997769A (en) * | 2015-07-24 | 2015-10-28 | 华中农业大学 | Applications of mangiferin in preparation of antiviral drugs |
| US20190177354A1 (en) * | 2016-04-29 | 2019-06-13 | Jinan University | Crocins Compounds and Uses Thereof |
| CN112552169A (en) * | 2020-12-08 | 2021-03-26 | 江西省科学院应用化学研究所 | Crocetin diester compound and preparation method and application thereof |
| US20210106680A1 (en) * | 2018-03-27 | 2021-04-15 | The University Of Tokyo | Adjuvant and vaccine containing adjuvant |
| WO2022206580A1 (en) * | 2021-04-02 | 2022-10-06 | 青岛清原化合物有限公司 | Ppo polypeptide having tolerance to ppo inhibitor herbicide, and application |
| DE202023100017U1 (en) * | 2023-01-03 | 2023-02-17 | Ravi Dhondiraj Barde | Novel drug for the prevention and treatment of bacterial mixed infections in freshwater fish |
-
2023
- 2023-05-29 CN CN202310618446.XA patent/CN116617238B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019539A (en) * | 2007-02-27 | 2007-08-22 | 南开大学 | Interacting composition possessing activity of resisting tobacco mosaic virus |
| CN104997769A (en) * | 2015-07-24 | 2015-10-28 | 华中农业大学 | Applications of mangiferin in preparation of antiviral drugs |
| US20190177354A1 (en) * | 2016-04-29 | 2019-06-13 | Jinan University | Crocins Compounds and Uses Thereof |
| US20210106680A1 (en) * | 2018-03-27 | 2021-04-15 | The University Of Tokyo | Adjuvant and vaccine containing adjuvant |
| CN112552169A (en) * | 2020-12-08 | 2021-03-26 | 江西省科学院应用化学研究所 | Crocetin diester compound and preparation method and application thereof |
| WO2022206580A1 (en) * | 2021-04-02 | 2022-10-06 | 青岛清原化合物有限公司 | Ppo polypeptide having tolerance to ppo inhibitor herbicide, and application |
| DE202023100017U1 (en) * | 2023-01-03 | 2023-02-17 | Ravi Dhondiraj Barde | Novel drug for the prevention and treatment of bacterial mixed infections in freshwater fish |
Non-Patent Citations (1)
| Title |
|---|
| 周江妍;宋晓园;徐黎贤;: "西红花苷对子宫腺肌病异位子宫内膜间质细胞生长周期的影响及机制研究", 中国现代医生, no. 23, pages 45 - 49 * |
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