CN116616450B - Anti-fatigue product - Google Patents
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- CN116616450B CN116616450B CN202310653538.1A CN202310653538A CN116616450B CN 116616450 B CN116616450 B CN 116616450B CN 202310653538 A CN202310653538 A CN 202310653538A CN 116616450 B CN116616450 B CN 116616450B
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Abstract
The invention discloses an anti-fatigue product, and belongs to the technical field of ocean efficacy food research and development. The preparation comprises oyster peptide rich in 3, 5-dihydroxy-4-methoxybenzyl alcohol (DHMBA); the content of DHMBA in oyster peptide is 430 μg/kg-650 μg/kg. In the preparation, the adding amount of the DHMBA-enriched oyster peptide is 0.5-3 wt%. The antifatigue product also comprises sea cucumber peptide, wherein the mass ratio of the oyster peptide rich in DHMBA to the sea cucumber peptide is 1:0.2-1:3. Experiments prove that the oyster peptide rich in DHMBA has good anti-fatigue activity, and the anti-fatigue effect is better through the compounding of the sea cucumber peptide.
Description
Technical Field
The invention belongs to the technical field of ocean efficacy food research and development, and particularly relates to an anti-fatigue product.
Background
Fatigue is a ubiquitous physiological phenomenon, and is a complex physiological and biochemical process. Persistent or severe fatigue not only affects normal life of people, but also causes endocrine disturbance, immunity degradation, even organic diseases, and threatens to health. Fatigue often occurs with changes in energy substances, metabolites, hormone levels, cellular metabolic regulation enzymes, and antioxidant system enzymes, and is essentially the loss of homeostasis that causes discomfort to the body. Methods for relieving fatigue include sleeping, warm water bath, physiotherapy, and supplementing nutritional food and medicine.
In recent years, many scholars are devoted to anti-fatigue research, and different from medicines for achieving the purpose of resisting fatigue, food-borne anti-fatigue active ingredients are more concerned because of basically no side effect on organisms, but no non-medicine effective anti-fatigue products are marketed at present.
Disclosure of Invention
The invention aims to provide an anti-fatigue product based on oyster peptide rich in 3, 5-dihydroxyl-4-methoxyl benzyl alcohol, which overcomes the defects of the prior art.
In order to achieve the purpose, the invention adopts the following specific technical scheme:
an anti-fatigue product comprising oyster peptide enriched in 3, 5-dihydroxy-4-methoxybenzyl alcohol (DHMBA).
Further, DHMBA is contained in an amount of 430 μg/kg to 650 μg/kg.
Further, in the preparation, the oyster peptide rich in DHMBA is added in an amount of 0.5% -3% (mass percent).
Further, the anti-fatigue product also comprises sea cucumber peptide.
Further, in the product, the mass ratio of the oyster peptide rich in DHMBA to the sea cucumber peptide is 1:0.2-1:3.
Further, the preparation of the DHMBA-enriched oyster peptide comprises the following steps: sterilizing homogenate of oyster meat which does not contain DHMBA, adding at least one of lactobacillus and saccharomycete, fermenting, steaming at high temperature, and drying.
The method comprises the following steps: sterilizing the oyster meat homogenate which does not contain DHMBA at 121 ℃ for 10-20min to obtain sterile oyster homogenate, fermenting at 31-45 ℃ for more than 6h by adding at least one of lactic acid bacteria with the mass percentage of 0.01-2% or saccharomycetes with the mass percentage of 0.01-1%, steaming the fermentation liquor at more than 90 ℃ for more than 6h, centrifuging the steamed fermentation liquor, and spray drying to obtain the oyster peptide rich in DHMBA.
Further, the preparation method of the sea cucumber peptide comprises the following steps: (1) the sea cucumber is steamed and crushed into slurry, and water is added for uniform mixing; (2) Adding compound protease into the sea cucumber homogenate for enzymolysis; (3) centrifuging the sea cucumber enzymolysis liquid to obtain sea cucumber clarifying liquid; (4) Concentrating the sea cucumber clarified liquid by double-effect evaporation to obtain sea cucumber concentrate; and (5) carrying out spray drying on the sea cucumber concentrate to obtain sea cucumber peptide powder.
Further, in the preparation method of the sea cucumber peptide powder, the compound protease in the step (2) comprises one or a mixture of more than one of neutral protease, papain, animal protease and alkaline protease.
Further, the anti-fatigue product comprises the following raw materials in parts by weight: 10-30 parts of sea cucumber peptide powder, 5-30 parts of oyster peptide rich in DHMBA, 5-15 parts of resistant dextrin, 1-3 parts of raspberry powder, 1-5 parts of momordica grosvenori extract and 5-17 parts of xylitol.
The oyster peptide rich in DHMBA can be applied to the preparation of anti-fatigue products.
The anti-fatigue product comprises powder, tablet, liquid product and the like.
The invention has the advantages and beneficial effects that:
experiments prove that the oyster peptide rich in DHMBA has good anti-fatigue activity, and the anti-fatigue effect is better through the compounding of the sea cucumber peptide. The content of the DHMBA-enriched oyster peptide DHMBA prepared by the invention is higher, the content of the prepared sea cucumber peptide protein reaches more than 90%, the purity is high, the molecular weight of the sea cucumber peptide is below 1000Da, and the sea cucumber peptide is a small molecular peptide and is easier to absorb.
The anti-fatigue product prepared based on DHMBA-enriched oyster peptide or marine active peptide has extremely high nutritive value, and the fishy smell of most marine products can be covered by compounding raspberry powder, grosvenor momordica fruit and the like, so that the anti-fatigue product has wide market prospect.
Detailed Description
The following non-limiting examples will enable those of ordinary skill in the art to more fully understand the application and are not intended to limit the application in any way. The following is merely exemplary of the scope of the application as claimed and many variations and modifications of the application will be apparent to those skilled in the art in light of the disclosure, which are intended to be within the scope of the application as claimed.
The invention is further illustrated by means of the following specific examples. The various chemical reagents used in the examples of the present invention were obtained by conventional commercial means unless otherwise specified.
Example 1
A preparation method of DHMBA-enriched oyster peptide comprises the following steps:
Removing shell of Concha Ostreae, taking edible part, adding water according to the ratio of Concha Ostreae to water (M: M=1:1), homogenizing for 10min by high pressure homogenizer to obtain Concha Ostreae homogenate; sterilizing Concha Ostreae homogenate at 121deg.C for 20min to obtain sterile Concha Ostreae homogenate; adding lactobacillus with mass percent of 1.5% into the above solution, fermenting at 37 ℃ for 72 hours to obtain oyster fermentation broth, steaming the oyster fermentation broth at 95 ℃ for 6.5 hours, cooling, centrifuging to obtain supernatant, and spray-drying to obtain oyster peptide rich in DHMBA.
Example 2
A preparation method of DHMBA-enriched oyster peptide comprises the following steps:
Removing shell of Concha Ostreae, taking edible part, adding water according to the ratio of Concha Ostreae to water (M: M=1:1), homogenizing for 10min by high pressure homogenizer to obtain Concha Ostreae homogenate; sterilizing Concha Ostreae homogenate at 121deg.C for 15min to obtain sterile Concha Ostreae homogenate, adding yeast with mass of 0.1% of the homogenate, fermenting at 42deg.C for 48 hr to obtain Concha Ostreae fermentation broth, steaming at 100deg.C for 8 hr, cooling, centrifuging to obtain supernatant, and spray drying to obtain Concha Ostreae peptide rich in DHMBA.
Example 3
A preparation method of DHMBA-enriched oyster peptide comprises the following steps:
Removing shell of Concha Ostreae, taking edible part, adding water according to the ratio of Concha Ostreae to water (M: M=1:1), homogenizing for 10min by high pressure homogenizer to obtain Concha Ostreae homogenate; sterilizing Concha Ostreae homogenate at 121deg.C for 15min to obtain sterile Concha Ostreae homogenate, adding yeast 0.7% and lactobacillus 2.0% of the homogenate, and adding fermentation bacteria in the following order: adding yeast with the mass of 0.2% and lactobacillus with the mass of 0.5% into the homogenate at 37 ℃ for fermentation for 12 hours, heating to 50 ℃, adding yeast with the mass of 0.5% and lactobacillus with the mass of 1.2% into the homogenate for fermentation for 24 hours, heating to 50 ℃, adding lactobacillus with the mass of 0.3% into the homogenate, and fermenting for 12 hours to obtain oyster fermentation broth; steaming at 100deg.C for 10 hr, cooling, centrifuging to obtain supernatant, and spray drying to obtain oyster peptide rich in DHMBA.
Example 4
A method for preparing sea cucumber peptide comprises:
Removing viscera of sea cucumber, steaming the body wall, adding water according to the ratio of sea cucumber to water (M: M=1:2), homogenizing for 5min by a high-pressure homogenizer, regulating the homogenate to 45 ℃, adding compound protease (neutral protease and papain) for enzymolysis for 1h, heating to 80 ℃ for 3min, cooling to 60 ℃, filtering by a tube-type centrifuge, obtaining sea cucumber clarifying solution, concentrating to 40% of solid by double-effect evaporation, and spray drying to obtain sea cucumber peptide.
Example 5
After the whole sea cucumber is steamed, adding water according to the proportion of the sea cucumber to water (M: M=1:2), homogenizing for 8min by a high-pressure homogenizer, regulating the homogenate to 50 ℃, regulating the pH to 9, adding compound protease (alkaline protease and animal protease) for enzymolysis for 2.5h, heating to 90 ℃ for 5min, cooling to 60 ℃, filtering by a disc centrifuge, obtaining a sea cucumber clarified liquid, concentrating by double-effect evaporation until the solid content is 55%, and spray drying to obtain the sea cucumber peptide.
Example 6
Removing viscera of sea cucumber, steaming the body wall, adding water according to the ratio of sea cucumber to water (M: M=1:2), homogenizing for 3min by a high-pressure homogenizer, regulating the homogenate to 40 ℃, adding papain for enzymolysis for 6h, heating to 95 ℃, keeping for 1min, cooling to 60 ℃, filtering by a plate-frame centrifuge to obtain a sea cucumber clarified liquid, concentrating by double-effect evaporation until the solid content is 47%, and spray drying to obtain sea cucumber peptide.
Example 7
A preparation method of common oyster peptide comprises the following steps: removing shell of oyster, taking edible part, adding water according to the proportion of oyster to water (M: M=1:1), homogenizing for 10min by a high-pressure homogenizer to obtain oyster homogenate, regulating the homogenate to 44 ℃, adding neutral protease and papain for enzymolysis for 6h, heating to 95 ℃ for 3min, cooling to 60 ℃, filtering by a plate-frame centrifuge to obtain oyster clarified liquid, concentrating by double-effect evaporation until the solid content is 47%, and spray drying to obtain oyster peptide powder.
Results determination:
Detecting the content of DHMBA by adopting a liquid chromatography-mass spectrometry combined method aiming at the DHMBA-enriched oyster peptide obtained in the examples 1-3, the sea cucumber peptide obtained in the examples 4-6 and the common oyster peptide prepared in the example 7; the sea cucumber peptide, common oyster peptide protein and relative molecular weight are detected by adopting a Soxhlet extraction method and a high-efficiency size exclusion chromatography. The content is determined as follows:
TABLE 1 DHMBA content detection results
TABLE 2 protein and molecular weight detection results
。
As can be seen from tables 1 and 2, in examples 1-3, the content of DHMBA can be significantly increased by both lactic acid bacteria and yeast, but the content of DHMBA is higher when the lactic acid bacteria and the yeast act together, which indicates that the lactic acid bacteria and the yeast have a synergistic effect, and the protein content is about 45 g/100 g-60 g/100g; in example 7, the common oyster peptide was not subjected to fermentation and high-temperature extraction, and only protein was subjected to enzymolysis, so that DHMBA content was only 13.8. Mu.g/kg.
The sea cucumber peptide extracted according to the embodiment has the protein content reaching more than 90%, and the molecular weight of the peptide is 100% below 1000Da, which proves that the extraction method can completely decompose the protein into small molecular peptide.
Example 8
A mouse anti-fatigue experiment was performed based on DHMBA-enriched oyster peptides, sea cucumber peptides and common oyster peptides prepared in examples 1-7.
1. Experimental animals: healthy, qualified SPF-grade, 8 week old male ICR mice, 48, purchased from Jinan Pengyue laboratory animal breeding Inc.
2. The experimental method comprises the following steps: after 1 week of adaptive feeding, mice (8 weeks of age) were randomly divided into six groups of 8 animals each. A first group: normal control group, lavage saline (100 mg/kg); second group: the DHMBA-enriched oyster peptide obtained in example 3 was prepared into a solution of 100mg/kg, and the mice were subjected to gastric lavage treatment; third group: preparing the sea cucumber peptide obtained in the example 5 into a solution of 100mg/kg, and performing gastric lavage treatment on the mice; fourth group: uniformly mixing the oyster peptide rich in DHMBA in the example 1 and the sea cucumber peptide in the example 4 according to the mass ratio of 1:1, preparing 100mg/kg solution, and performing gastric lavage treatment on the mice; fifth group: the DHMBA-enriched oyster peptide obtained in example 2 and the sea cucumber peptide obtained in example 6 were uniformly mixed according to a mass ratio of 1:0.5, and then 100mg/kg of solution was prepared, and the mice were subjected to gastric lavage treatment. Sixth group: the common oyster peptide in example 7 was prepared as a 100mg/kg solution, and the mice were subjected to gastric lavage treatment. 30 After min, the exhaustion pole climbing experiment and the exhaustion running experiment are carried out.
(1) Experiment of climbing pole of resina Draconis
The pole-climbing frame adopts a glass rod with the length of 40 cm and the diameter of 8 mm, the lower end is suspended perpendicular to the ground, the distance from the ground is 25 cm, and the upper end is reserved with 5.5 cm. Recording the exhaustion time of the mice, performing pole climbing training on the mice once before formal experiments, repeating the experiments for 3 times, and taking the average value of the residence time of the pole climbing experiments for 3 times as the final residence time. The creeper was cleaned after each test.
(2) Running experiment of exhaustion
The mice are placed in a wheel-rotation type fatigue tester for running with medium strength as parameters. The mice can not continue running, stay at the rear part of the running track, breathe fast and tremble muscles, still can not continue running to be in a exhausted state after electric shock, and the exhausted running duration is recorded.
3. Experimental results
TABLE 3 time of the exhaustion of mice of different groups
TABLE 4 running time of the exhaustion of mice of different groups
The exercise endurance test is the most direct mode for detecting the anti-fatigue capability of the mice, and the exercise fatigue degree of the mice can be reflected by the length of the exhausted climbing pole and the exhausted running time. The experiment adopts the exhaustion pole-climbing and running experiment to test the anti-fatigue capability of mice, as shown in table 3, compared with the first group, the exhaustion pole-climbing time of the normal model mice of the second group, the third group, the fourth group, the fifth group and the sixth group is increased, the difference is obvious (P < 0.05), and the exhaustion pole-climbing time of the mice of the second group is higher than that of the mice of the third group; the fourth group of mice and the fifth group of mice are irrigated with the solution compounded by the oyster peptide rich in DHMBA and the sea cucumber peptide, and the gastric lavage rate is obviously higher than that of the second group of mice and the third group of mice (P is less than 0.05); the exhaustion pole-climbing time of the fourth group of mice is longer than that of the fifth group, which proves that the oyster peptide rich in DHMBA plays a main role in resisting fatigue; according to the experimental result of the sixth group of mice, the addition of the common oyster peptide can improve the anti-fatigue capability of the mice, but has weaker anti-fatigue effect than the oyster peptide rich in DHMBA. Meanwhile, as shown in Table 4, the exhaustion running time of the second and third groups of mice is 1.6-1.8 times longer than that of the first group, the exhaustion running time of the second group of mice is longer than that of the third group, the exhaustion running time of the fourth, fifth and sixth groups of mice is higher than that of the first three groups, the exhaustion running time of the fourth group of mice is about 1.9-2.4 times longer than that of the first group, and the exhaustion running endurance of the fourth group of mice is the longest. It can be seen that the anti-fatigue product can be added with the oyster peptide rich in DHMBA, the sea cucumber peptide and the oyster peptide to play a role in resisting fatigue, the anti-fatigue effect of the mice is more obvious after the mice are subjected to gastric lavage by using the oyster peptide rich in DHMBA and the sea cucumber peptide compound product, and the anti-fatigue effect of the common oyster peptide is better than that of the sea cucumber peptide alone, but is lower than that of the oyster peptide rich in DHMBA.
The DHMBA-enriched oyster peptide prepared by the method has good anti-fatigue effect and is obviously superior to common oyster peptide.
Example 9
An antifatigue solid beverage: 15 parts of the oyster peptide rich in DHMBA parts of the oyster peptide prepared in the example 2, 10 parts of the sea cucumber peptide prepared in the example 4, 5 parts of resistant dextrin, 3 parts of raspberry powder, 2 parts of a momordica grosvenori extract and 13 parts of xylitol are taken according to mass fraction, and the powder is uniformly mixed to obtain the anti-fatigue solid beverage.
Example 10
An antifatigue solid beverage: 15 parts of the oyster peptide rich in DHMBA prepared in example 5, 30 parts of the sea cucumber peptide prepared in example 4, 6 parts of resistant dextrin, 1 part of raspberry powder, 1 part of momordica grosvenori extract and 15 parts of xylitol are taken according to mass fraction, and the powder is uniformly mixed to obtain the anti-fatigue solid beverage.
The beverages prepared in examples 9 and 10 were subjected to sensory evaluation in terms of color, smell, taste, and uniformity, as shown in table 5:
table 5 organoleptic evaluation of the anti-fatigue articles of examples 9, 10
As can be seen from Table 5, the anti-fatigue product added with the oyster peptide and the sea cucumber peptide which are rich in DHMBA has good characteristic flavor and is easy to accept; the prepared anti-fatigue solid product has good uniformity after being brewed, has no sediment and layering, namely good solubility and easy absorption, and the raspberry aroma and the sour and sweet taste can mask the fishy smell of the seafood to a certain extent, so that the anti-fatigue solid product is more easily accepted by consumers. Namely, the DHMBA-enriched oyster peptide prepared by the invention can be applied to the preparation of anti-fatigue products to carry out market popularization.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any person skilled in the art may make modifications or alterations to the above technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (1)
1. An anti-fatigue product comprising oyster peptide enriched in 3, 5-dihydroxy-4-methoxybenzyl alcohol; the content of DHMBA in oyster peptide is 430 μg/kg-650 μg/kg; the addition amount of the oyster peptide rich in DHMBA wt% to 3wt%; the product also comprises sea cucumber peptide; the mass ratio of the oyster peptide rich in DHMBA to the sea cucumber peptide is 1:1; the oyster peptide rich in DHMBA is prepared by sterilizing oyster meat homogenate which does not contain DHMBA per se at 121 ℃ for 10-20min to obtain sterile oyster homogenate, wherein the addition mass percentage of lactobacillus is 0.01% -2%, the addition amount of saccharomycetes is 0.01% -1%, fermenting for more than 6h at 31-45 ℃, steaming the fermentation liquor at more than 90 ℃ for more than 6h, centrifuging the steamed fermentation liquor, and spray drying to obtain oyster peptide rich in DHMBA; the preparation method of the sea cucumber peptide comprises the following steps: removing viscera of sea cucumber, steaming the body wall, adding water according to the mass ratio of sea cucumber to water of 1:2, homogenizing for 5min by a high-pressure homogenizer, regulating the homogenate to 45 ℃, and adding compound protease: and (3) carrying out enzymolysis on neutral protease and papain for 1h, heating to 80 ℃ and keeping for 3min, cooling to 60 ℃ and filtering by a tube-type centrifuge to obtain a sea cucumber clear liquid, concentrating by double-effect evaporation until the solid content is 40%, and spray-drying to obtain the sea cucumber peptide.
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