CN116593465B

CN116593465B - Method and device for detecting pathogenic microorganisms

Info

Publication number: CN116593465B
Application number: CN202310869531.3A
Authority: CN
Inventors: 张勇
Original assignee: Guangzhou Yuanguji Technology Co ltd
Current assignee: Guangzhou Yuanguji Technology Co ltd
Priority date: 2023-07-17
Filing date: 2023-07-17
Publication date: 2023-10-27
Anticipated expiration: 2043-07-17
Also published as: CN116593465A

Abstract

The invention relates to the technical field of microorganism detection, and particularly provides a method and a device for detecting pathogenic microorganisms. This detection device of pathogenic microorganism includes protection subassembly, sampling subassembly, vibration subassembly, remove the subassembly, stoving dyeing subassembly and detection subassembly, protection subassembly includes the work base station, protecting sheathing, place the backup pad, place board and revolving door, work base station fixed mounting is subaerial, protecting sheathing fixed mounting is in the top of work base station, through utilizing inertial action, thereby improve the area of fungus liquid on the slide glass, improve the diffusion area of fungus liquid, prevent that fungus liquid from piling up and form the drop of water on the surface of slide glass, can make fungus liquid form a relatively even thin layer on the slide glass, better detection analysis carries out, accuracy and accuracy when having improved the detection, practicality and detection efficiency have been improved.

Description

Method and device for detecting pathogenic microorganisms

Technical Field

The invention relates to the technical field of microorganism detection, in particular to a method and a device for detecting pathogenic microorganisms.

Background

Pathogenic microorganisms are those microorganisms capable of causing diseases, which can invade the human or animal body to cause various degrees of damage through various transmission routes, including infectious diseases, parasitic diseases, and the like. The specific type and hazard degree of pathogenic microorganisms depend on various factors such as regions, environments, nutritional conditions, body immunity states and the like, and a key task is performed in the detection of the pathogenic microorganisms, so that infectious diseases can be prevented, controlled and treated. At present, detection of pathogenic microorganisms requires manual sampling by an operator, and the operation steps are complicated and complicated for the operator.

Disclosure of Invention

Based on this, it is necessary to provide a method and a device for detecting pathogenic microorganisms, so as to solve at least one technical problem set forth in the above background art.

In order to achieve the above purpose, the present invention provides the following technical solutions:

the utility model provides a detection device of pathogenic microorganism, including the protection subassembly, the sampling subassembly, vibrating subassembly, remove the subassembly, stoving dyeing subassembly and detection subassembly, the protection subassembly includes the work base station, the protective housing, place the backup pad, place board and revolving door, work base station fixed mounting is subaerial, protective housing fixed mounting is in the top of work base station, the open slot has been seted up to a side wall tip of protective housing, place backup pad fixed mounting in the top of work base station one end, place the top of board horizontal fixed mounting in place the backup pad, revolving door rotation is installed on the lateral wall of open slot, sampling subassembly fixed mounting is in the top of work base station, and be located one side of placing the board, vibrating subassembly installs in the top of work base station and is located one side of sampling subassembly, remove subassembly fixed mounting in the top of work base station, and be located vibrating subassembly and keep away from the top of placing the board, detecting subassembly fixed mounting is in the top of work base station, and be located the one side that the vibrating subassembly was kept away from to stoving dyeing subassembly.

In one embodiment, a circular placing groove is formed in the top of the placing plate, a placing bottle is placed on the placing groove, an L-shaped sampling supporting block is fixedly installed on the placing plate, one end of the sampling supporting block is fixedly installed on the placing plate, the other end of the sampling supporting block extends to the upper side of the placing bottle, an L-shaped supporting plate is fixedly installed on the top of the working base, an L-shaped extrusion supporting block is fixedly installed on the top of the supporting plate, one end of the extrusion supporting block is fixedly installed on the working base, and the other end of the extrusion supporting block extends to the direction of the rotating door.

In one embodiment, the sampling assembly comprises a sampling support plate, a sampling motor, a sampling rotating shaft, a sampling rotating arm, a connecting shaft, a sampling piece and a sampling gear, wherein the sampling support plate is fixedly arranged at the top of the working base, the sampling motor is fixedly arranged on one side wall of the sampling support plate, an output shaft of the sampling motor penetrates through the sampling support plate, the sampling rotating shaft is fixedly arranged on the output shaft of the sampling motor, one end of the sampling rotating arm is fixedly arranged in the middle of the sampling rotating shaft, one end of the connecting shaft is rotatably arranged at the other end of the sampling rotating arm, the sampling piece is fixedly arranged at the other end of the connecting shaft, and the sampling gear is fixedly arranged at one end of the sampling rotating shaft far away from the sampling support plate.

In one embodiment, the sampling piece includes sampling bottle and sampling pipe, and the top fixed mounting of sampling bottle lateral wall is on the tip of sampling pivot, and sampling pipe fixed mounting is in the bottom of sampling bottle, and the inside intercommunication of sampling bottle and sampling pipe, the lateral wall of sampling bottle bottom are the rubber material, and the sampling is supported and is held the piece and is supported the piece and all can support the bottom that holds the extrusion sampling bottle and make its compression deformation with the extrusion.

In one embodiment, the protruding two rack limiting plates that are equipped with in top of work base station, two rack limiting plates all are located the below of sampling gear, vibration subassembly includes linkage rack, vibration sand grip, two vibration backup pads, vibrating shoe and plummer, linkage rack slidable mounting is in the top of work base station, and be located between two rack limiting plates, the top and the sample gear intermeshing of linkage rack, vibration sand grip fixed mounting keeps away from the bottom of placing plate one end in the linkage rack, and vibration sand grip can slide at the top of work base station, the top of vibration sand grip is upwards protruding to be equipped with a plurality of arc lug, two equal fixed mounting in the top of work base station, and be located the both sides of vibration sand grip respectively, the spring groove has all been seted up on the lateral wall that two vibration backup pads are mutual towards, the top fixed mounting of spring groove has vibration to push down the spring, the both sides fixed mounting of vibrating shoe has two lower briquetting, the vibrating shoe is through two lower briquetting slidable mounting in between two vibration backup pads, and the top of two lower briquetting respectively with the bottom interconnect of two lower pressure springs, the bottom interconnect of vibration piece, a plurality of dovetail joint has been seted up with the bottom of vibration piece, set up with the top of work base station at the top of work base station and can slide, and a plurality of arc lug is located the arc lug in parallel groove, and the arc lug is located in the arc lug groove is worn to the arc groove, and is parallel to the arc lug groove has been located in the arc lug groove.

In one embodiment, square mounting groove has been seted up at the top of plummer, the slide glass is installed to the bottom of mounting groove, two Z-shaped grooves have been seted up to the symmetry on the lateral wall of mounting groove orientation two vibration backup pad, equal slidable mounting has the Z shape fixture block in two Z-shaped grooves, reset groove has all been seted up on the lateral wall that two Z-shaped grooves deviate from each other, reset inslot fixed mounting has reset spring, reset spring's one end fixed mounting is on the lateral wall of mounting groove, other end fixed mounting is in the middle part of Z-shaped fixture block, the one end that Z-shaped fixture block is close to each other all supports to hold in the top of slide glass, the top of plummer is close to the both sides of two vibration backup pads and has all been seted up the bar groove, and two bar grooves communicate each other with corresponding Z-shaped groove respectively.

In one embodiment, the moving assembly comprises a moving connecting plate, a motor mounting table, a moving motor, two guide supporting plates, two guide rods, a moving threaded rod and a moving block, wherein the moving connecting plate is fixedly mounted on the side wall of the two vibrating supporting plates facing the sampling gear, the motor mounting table is fixedly mounted on the top of the workbench far away from one end of the placing plate, the moving motor is fixedly mounted on the top of the motor mounting table, the two guide supporting plates are fixedly mounted on the top of the motor mounting table and are positioned on two sides of an output shaft of the moving motor, one ends of the two guide rods are respectively fixedly mounted on the side wall of the two guide supporting plates, the other ends of the two guide rods are respectively fixedly mounted on the side wall of the moving connecting plate, one end of the moving threaded rod is fixedly mounted on the output shaft of the moving motor, the other ends of the guide rods are rotatably mounted on the side wall of the moving connecting plate, the moving block is sleeved on the moving threaded rod, the two guide rods are respectively penetrated through the moving block, the bottom of the bearing table is provided with a movable groove, the bottom of the bearing table is downwards provided with two clamping blocks, the moving block is arranged in the movable groove in a protruding mode, the moving block is located in the movable groove, the top of the moving block is provided with two clamping grooves, and the two clamping grooves are respectively inserted in the two linkage clamping grooves.

In one embodiment, the drying and dyeing assembly comprises a drying box body, a dyeing supporting plate, a dyeing connecting plate, a dyeing device and an inductor, wherein the drying box body is fixedly installed on a working base station and is located at one side of a vibration convex strip far away from a linkage rack, a through groove is formed in the middle of the drying box body, two guide rods and a movable threaded rod penetrate through the through groove, one end of the dyeing supporting plate is fixedly installed on the working base station and located at one side of the drying box body far away from the vibration convex strip, the other end of the drying box body extends towards the upper side of the movable threaded rod, the dyeing connecting plate is fixedly installed at the extending end of the dyeing supporting plate, the dyeing device is fixedly installed at the bottom of the dyeing connecting plate, and the inductor is fixedly installed at the bottom of the dyeing connecting plate and located at one side of the dyeing device far away from the drying box body.

In one of them embodiment, the one end that two Z shape fixture blocks kept away from each other is formed with the tight inclined plane of clamp, detect the subassembly and include four detection supporting legs, detect the supporting bench, detect the cylinder, detect the device, detect the probe, two rotating plates, two translation fly leaves, two positioning fixture blocks and two are scribbled the pulling piece, four detection supporting legs fixed mounting is in the top of work base, detect the supporting bench fixed mounting in the top of four detection supporting legs, and be located the top of two guide bars and removal threaded rod, detect the cylinder fixed mounting in the top of detecting the supporting bench, and detect the output of cylinder and run through and detect the supporting bench downwardly extending, detect the device fixed mounting on the output of detecting the cylinder, detect the probe fixed mounting on detecting the device, two rotating plates rotate respectively and install in the bottom of detecting the probe both sides wall, and the rotating plate sets up perpendicularly with removal threaded rod, the one end that two translation fly leaves each other is installed in the bottom of two rotating plates through the torsional spring rotation, and the bottom of translation fly leaf is provided with translation spout, two positioning fixture blocks respectively slide card locate in the translation spout of two translation fly leaves respectively, translation spout inner mounting has the bottom to keep the bottom of two translation supporting slots to keep in order to bear the bottom of two translation supporting slots to keep the top of the side wall of the device, can be fixed to draw in the bottom of two translation supporting plate, and top of the bottom of translation groove is held in translation top of the device.

The invention also provides a detection method of pathogenic microorganisms, the detection method using the detection device of pathogenic microorganisms as described above, the detection method comprising the steps of:

step one: the operator opens the rotating door, pour fungus liquid into and place in the bottle, then close the rotating door, start the sampling motor, drive the sampling pivot through the sampling motor and rotate and make the sampling bottle keep away from the sample and support the piece, make the fungus liquid of bottom sucked into the sampling tube, meanwhile, the sampling pivot drives the sampling gear to rotate, the sampling gear rotates and drives the linkage rack to move, the linkage rack moves and drives the vibration sand grip to move, the vibration sand grip moves and drives a plurality of arc-shaped protruding blocks to move, a plurality of arc-shaped protruding blocks move and can support and drive the vibration block to move up and down through the trapezoid slot, the vibration block drives the plummer to vibrate up and down, when the sampling bottle moves to and supports and presses the extrusion and support the piece, the extrusion supports and supports the bottom of the extrusion sampling bottle, thus extrude fungus liquid in the sampling tube and drop on the glass slide, and stop the sampling motor;

step two: starting a sampling motor to reversely rotate, enabling a sampling rotating shaft and a sampling gear to reversely rotate, enabling a sampling gear to drive a linkage rack to move towards a direction close to a placing plate, enabling a linkage rack to drive a vibration convex strip to move towards the direction close to the placing plate, enabling a vibration convex strip to move to drive a vibration block to repeatedly vibrate up and down, enabling a bearing table to vibrate by the vibration block, enabling a bacterial colony to be more uniformly dispersed on the glass slide, and then stopping the sampling motor;

Step three: starting a moving motor, wherein the moving motor drives a moving threaded rod to rotate, two guide rods limit a moving block, the moving threaded rod rotates to drive the moving block to move, and the moving block moves to drive a bearing table to move, so that a glass slide moves, and when the moving block and the bearing table move into a drying box body, the moving motor is stopped, and the drying box body heats and dries bacterial liquid on the glass slide;

step four: after drying, the moving motor is continuously started, the moving block continuously drives the carrying table and the glass slide to move, when the glass slide moves below the dyeing device, the moving motor is stopped, the sensor senses that the glass slide stays, and the dyeing device drops the dyeing agent onto the glass slide to dye colonies on the glass slide;

step five: after dropping the coloring agent, continue to start the movable motor for the movable block continues to move, stop the movable motor when the movable block drives the slide glass on the plummer to move to under the test probe, start the detection cylinder, the detection device is driven to move down by the detection cylinder, the detection device moves down and drives the rotating plate and the translation fly leaf to move down, make the positioning fixture block get into and support in the groove that supports that corresponds, two translation fly leaves drive two and smear the slide to move to the direction that keeps away from each other, smear the coloring agent to both sides evenly, two translation fly leaves the top of slide after keeping away from each other, the test probe moves down to the top of slide and detects the colony on the slide, the detection device detects the analysis through the test probe to the colony above the slide.

Compared with the prior art, the invention has the beneficial effects that:

1. utilize inertial action for fungus liquid in the sampling tube has the trend of downwardly moving, thereby make fungus liquid drip on the slide glass with great dynamics, the fungus liquid of drip on the slide glass can outwards diffuse, thereby improve the area of fungus liquid on the slide glass, improve the diffusion area of fungus liquid, prevent that fungus liquid from piling up and forming the drop of water on the surface of slide glass, can make fungus liquid form a relatively even thin layer on the slide glass, better detection analysis, accuracy and accuracy when having improved the detection, practicality and detection efficiency have been improved.

2. The up-and-down vibration of the vibration block can vibrate up and down on the movable bearing table, the up-and-down vibration of the bearing table can drive the slide glass to vibrate up, the up-and-down vibration of the slide glass can lead the upper surface of the slide glass to carry positive or negative charges, microorganisms and other components in the bacterial liquid also carry positive or negative charges, and electrostatic attraction force can be generated when the microorganisms and other components fall on the slide glass with opposite charges, so that microcosmic friction force is improved, and bacterial colonies in the bacterial liquid are more easy to attach and fix on the slide glass;

the surface tension between the small liquid drops of the bacterial liquid is reduced through vibration, the bacterial liquid is ensured to be evenly distributed on the surface of the glass slide, so that bacterial colonies can be more evenly dispersed on the glass slide, the vibration glass slide can also help the bacterial liquid to better permeate into holes of the glass slide, thereby being beneficial to the fixation and collection of bacterial colonies, and the glass slide can promote the bacterial liquid on the glass slide to be evenly distributed and fixed on the glass slide, and the experimental precision and reliability are improved.

3. Bacterial liquid on the glass slide is dried, bacterial colonies can be better fixed and stored, bacterial colonies are prevented from being damaged and shifted before or during dyeing, adhesion force between the bacterial colonies and the glass slide can be increased in the drying process, the dyeing agent is more uniformly distributed on the glass slide, phenomena of twisting, drifting and the like caused by liquid drops in the dyeing process can be avoided, and accuracy and good dyeing effect are ensured.

4. Can smear the colorant to both sides evenly, and prevent to smear the upper surface that drags piece damage bacterial colony and slide when scraping the colorant, two translation fly leaves the top that will leave the slide after keeping away from each other, and the test probe moves down to the top of slide and detects the bacterial colony on the slide, and the test device detects the analysis through the test probe to the bacterial colony of slide top, thereby realizes detecting and analyzing the pathogenic microorganism in the bacterial colony, has improved the accuracy and the practicality of detection.

Drawings

FIG. 1 is a schematic diagram of an embodiment of the present invention;

FIG. 2 is a schematic view of an embodiment of the present invention with the protective housing and the revolving door removed;

FIG. 3 is a schematic diagram of a sampling assembly, a vibrating assembly and a part of a moving assembly according to an embodiment of the present invention;

FIG. 4 is a schematic view showing a structure of a vibration supporting plate according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of a vibrating mass according to an embodiment of the present invention;

FIG. 6 is a schematic cross-sectional view of a stage and a moving block according to an embodiment of the invention;

fig. 7 is a schematic structural diagram of a detection component according to an embodiment of the invention.

In the figure: 10. a protective assembly; 20. a sampling assembly; 30. a vibration assembly; 40. a moving assembly; 50. drying the dyeing assembly; 60. a detection assembly; 11. a work base; 12. a protective housing; 13. placing a supporting plate; 14. placing a plate; 15. a rotating door; 121. an open slot; 141. placing a bottle; 142. a sampling supporting block; 111. supporting the supporting plate; 112. extruding the supporting block; 21. a sampling support plate; 22. a sampling motor; 23. a sampling rotating shaft; 24. a sampling rotating arm; 25. a connecting shaft; 26. sampling a sample piece; 27. a sampling gear; 261. sampling bottle; 262. a sampling tube; 113. a rack limiting plate; 31. a linkage rack; 32. vibrating the convex strips; 33. a vibration support plate; 34. a vibrating block; 35. a carrying platform; 321. arc-shaped protruding blocks; 331. a spring groove; 332. pressing down the spring; 341. pressing the block; 342. a trapezoid groove; 343. a convex groove; 351. a mounting groove; 36. a glass slide; 353. a Z-shaped groove; 354. a Z-shaped clamping block; 355. a reset groove; 356. a return spring; 357. a bar-shaped groove; 41. moving the connecting plate; 42. a motor mounting table; 43. a moving motor; 44. a guide support plate; 45. a guide rod; 46. moving the threaded rod; 47. a moving block; 358. a movable groove; 359. a linkage clamping block; 471. a linkage clamping groove; 51. drying the box body; 52. a dyeing support plate; 53. dyeing a connecting plate; 54. a dyeing device; 55. an inductor; 350. clamping inclined planes; 61. detecting supporting legs; 62. detecting a supporting table; 63. detecting a cylinder; 64. a detection device; 65. a detection probe; 66. a rotating plate; 67. translating the movable plate; 68. positioning a clamping block; 69. smearing a pulling piece; 671. translating the chute; 672. a lateral translation spring; 681. support and hold the groove.

Detailed Description

In order that the invention may be readily understood, a more complete description of the invention will be rendered by reference to the appended drawings. The drawings illustrate preferred embodiments of the invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

The invention provides a detection device for pathogenic microorganisms, as shown in fig. 1-7, the detection device comprises a protection component 10, a sampling component 20, a vibration component 30, a moving component 40, a drying and dyeing component 50 and a detection component 60, wherein the protection component 10 comprises a working base 11, a protection shell 12, a placing support plate 13, a placing plate 14 and a rotating door 15, the working base 11 is fixedly arranged on the ground, the protection shell 12 is fixedly arranged on the top of the working base 11, an opening groove 121 is formed in one side wall end of the protection shell 12, the placing support plate 13 is fixedly arranged on the top of one end of the working base 11, the placing plate 14 is horizontally and fixedly arranged on the top of the placing support plate 13, the rotating door 15 is rotatably arranged on the side wall of the opening groove 121, the sampling component 20 is fixedly arranged on the top of the working base 11 and is positioned on one side of the placing plate 14, the vibration component 30 is arranged on the top of the working base 11 and is positioned on one side of the sampling component 20, the moving component 40 is fixedly arranged on the top of the working base 11 and is positioned on one side of the vibration component 30, the dyeing component 50 is fixedly arranged on the top of the working base 11 and is positioned on one side of the drying component 30 and is far away from the side of the placing support plate 14 and is positioned on one side of the vibration component 50 and is far away from the vibration component 30.

The top of placing the board 14 has seted up circular shape standing groove, has placed the placing bottle 141 on the standing groove, and the fixed mounting of placing the board 14 has the sampling of L shape to hold the piece 142, and the one end fixed mounting of piece 142 is held in the placing on the board 14 to the sampling, and the other end extends to the top of placing the bottle 141, and the top fixed mounting of work base station 11 has the supporting board 111 that supports of L shape, supports the extrusion of supporting board 111 top fixed mounting and holds the piece 112 that supports of L shape, and the one end fixed mounting of block 112 is held in work base station 11 to the extrusion, and the other end extends to the direction of revolving door 15.

The sampling assembly 20 comprises a sampling support plate 21, a sampling motor 22, a sampling rotating shaft 23, a sampling rotating arm 24, a connecting shaft 25, a sampling piece 26 and a sampling gear 27, wherein the sampling support plate 21 is fixedly installed at the top of the workbench 11, the sampling motor 22 is fixedly installed on one side wall of the sampling support plate 21, an output shaft of the sampling motor 22 penetrates through the sampling support plate 21, the sampling rotating shaft 23 is fixedly installed on the output shaft of the sampling motor 22, one end of the sampling rotating arm 24 is fixedly installed in the middle of the sampling rotating shaft 23, one end of the connecting shaft 25 is rotatably installed at the other end of the sampling rotating arm 24, the sampling piece 26 is fixedly installed at the other end of the connecting shaft 25, and the sampling gear 27 is fixedly installed at the end part of the sampling rotating shaft 23 far away from the sampling support plate 21.

The sampling piece 26 comprises a sampling bottle 261 and a sampling tube 262, the top of one side wall of the sampling bottle 261 is fixedly arranged on the end part of the sampling rotating shaft 23, the sampling tube 262 is fixedly arranged at the bottom of the sampling bottle 261, the sampling bottle 261 is communicated with the inside of the sampling tube 262, the side wall of the bottom of the sampling bottle 261 is made of rubber, and the sampling propping block 142 and the extrusion propping block 112 can prop against the bottom of the extrusion sampling bottle 261 to enable the bottom to be compressed and deformed.

The top of work base station 11 is protruding to be equipped with two rack limiting plates 113, two rack limiting plates 113 all are located the below of sampling gear 27, vibration subassembly 30 includes linkage rack 31, vibration sand grip 32, two vibration backup pads 33, vibration piece 34 and plummer 35, linkage rack 31 slidable mounting is in the top of work base station 11, and be located between two rack limiting plates 113, the top and the sampling gear 27 intermeshing of linkage rack 31, vibration sand grip 32 fixed mounting is kept away from the bottom of placing plate 14 one end in linkage rack 31, and vibration sand grip 32 can slide at the top of work base station 11, the top of vibration sand grip 32 upwards protruding is equipped with a plurality of arc lug 321, two vibration backup pads 33 all fixed mounting are in the top of work base station 11, and be located the both sides of vibration sand grip 32 respectively, all set up spring groove 331 on the lateral wall that two vibration backup pads 33 are mutually oriented, spring groove 331's top fixed mounting has vibration down spring 332, two down briquetting 341 are fixedly installed to the both sides of vibration piece 34, vibration piece 34 is through two lower briquetting 341 slidable mounting in between two trapezoidal piece 33, and two trapezoidal piece 321 are kept away from the bottom of placing plate 14 one end, and vibration piece 342 is located the bottom of arc lug 343, the vibration piece is located the bottom of vibration piece 342 is located in the arc lug groove 343 is located the side wall of vibration groove 342, the vibration piece is located the bottom of the arc lug groove 342 is located the vibration groove is located the vibration lug groove is located the side by the vibration lug groove is located the side of the lug groove is located the bottom of the vibration lug groove is located, the vibration piece is located.

Square mounting groove 351 has been seted up at the top of plummer 35, slide glass 36 is installed to the bottom of mounting groove 351, two Z-shaped grooves 353 have been seted up to the symmetry on the lateral wall of mounting groove 351 orientation two vibration backup pad 33, equal slidable mounting has Z-shaped fixture block 354 in two Z-shaped grooves 353, reset groove 355 has all been seted up on the lateral wall that two Z-shaped grooves 353 deviate from each other, fixed mounting has reset spring 356 in the reset groove 355, reset spring 356's one end fixed mounting is on the lateral wall of mounting groove 351, other end fixed mounting is in the middle part of Z-shaped fixture block 354, the one end that Z-shaped fixture block 354 is close to each other all supports in slide glass 36's top, bar groove 357 has all been seted up to the both sides that the top of plummer 35 is close to two vibration backup pad 33, and two bar grooves 357 communicate each other with corresponding Z-shaped groove 353 respectively.

The moving assembly 40 comprises a moving connecting plate 41, a motor mounting table 42, a moving motor 43, two guide supporting plates 44, two guide rods 45, a moving threaded rod 46 and a moving block 47, wherein the moving connecting plate 41 is fixedly mounted on the side wall of the two vibration supporting plates 33 facing the sampling gear 27, the motor mounting table 42 is fixedly mounted on the top of one end of the working base table 11, which is far away from the placing plate 14, the moving motor 43 is fixedly mounted on the top of the motor mounting table 42, the two guide supporting plates 44 are fixedly mounted on the top of the motor mounting table 42 and are positioned on two sides of the output shaft of the moving motor 43, one ends of the two guide rods 45 are respectively fixedly mounted on the side wall of the two guide supporting plates 44, the other ends of the two guide rods 45 are respectively fixedly mounted on the side wall of the moving connecting plate 41, one end of the moving 46 is fixedly mounted on the output shaft of the moving motor 43, the other ends of the moving connecting plate 41 are rotatably mounted on the side wall of the moving connecting plate 41, the moving block 47 is in threaded sleeve on the moving block 46, the two guide rods 45 penetrate through the moving block 47, a movable groove 358 is formed in the bottom of the bearing table 35, two linkage clamping blocks 359 are downwards convexly provided, one ends of the moving table 35 are respectively, the two linkage clamping blocks 359 are respectively located in the movable grooves 358, the two linkage clamping grooves are respectively located in the movable grooves 358, and two linkage threaded grooves are respectively.

The drying and dyeing assembly 50 comprises a drying box body 51, a dyeing supporting plate 52, a dyeing connecting plate 53, a dyeing device 54 and an inductor 55, wherein the drying box body 51 is fixedly installed on the working base 11 and is located at one side of the vibration raised line 32 far away from the linkage rack 31, a through groove is formed in the middle of the drying box body 51, two guide rods 45 and a movable threaded rod 46 penetrate through the through groove, one end of the dyeing supporting plate 52 is fixedly installed on the working base 11 and is located at one side of the drying box body 51 far away from the vibration raised line 32, the other end of the dyeing supporting plate extends above the movable threaded rod 46, the dyeing connecting plate 53 is fixedly installed at the extending end of the dyeing supporting plate 52, the dyeing device 54 is fixedly installed at the bottom of the dyeing connecting plate 53, the inductor 55 is fixedly installed at the bottom of the dyeing connecting plate 53 and is located at one side of the dyeing device 54 far away from the drying box body 51.

The one end that two Z-shaped fixture blocks 354 kept away from each other is formed with and presss from both sides tight inclined plane 350, detect the subassembly 60 includes four detection supporting legs 61, detect supporting bench 62, detect cylinder 63, detect device 64, detect probe 65, two rotatory boards 66, two translation fly leaf 67, two positioning fixture block 68 and two and scribble and drag piece 69, four detection supporting legs 61 fixed mounting are in the top of workstation 11, detect supporting bench 62 fixed mounting is at the top of four detection supporting legs 61, and be located the top of two guide bars 45 and removal threaded rod 46, detect cylinder 63 fixed mounting is in the top of detecting supporting bench 62, and the output of detect cylinder 63 runs through detect supporting bench 62 downwardly extending, detect device 64 fixed mounting is on the output of detect cylinder 63, detect probe 65 fixed mounting is on detect device 64, two rotatory boards 66 are installed in the bottom of detect probe 65 both sides wall respectively, and rotatory board 66 and removal threaded rod 46 set up perpendicularly, the one end that two translation fly leaf 67 kept away from each other is installed in the bottom of two rotatory boards 66 through the torsional spring rotation respectively, and translation fly leaf 67's bottom is provided with the top of two translation fly leaf 67 and is provided with 671 two translation fly leaf 67 and is held the top 67 and is held the translation groove 67 and can be located the top of two translation draw piece 67 respectively, the translation groove 671 is held in the translation top 67 and two translation groove 67 translation top is equipped with the translation groove 67 is kept close to each other, the translation top of the top 67, the translation is equipped with the top of the two translation draw leaf 67, the top 67 is made in the translation draw-down in the translation groove is made.

The invention also provides a detection method of pathogenic microorganisms, which is applied to the detection device of pathogenic microorganisms and comprises the following steps:

step one: the operator opens the rotary door 15 to pour the bacterial liquid into the placing bottle 141, then closes the rotary door 15, starts the sampling motor 22, drives the sampling rotary shaft 23 to rotate through the sampling motor 22 to enable the sampling bottle 261 to be far away from the sampling supporting block 142, and enables the bacterial liquid at the bottom to be sucked into the sampling tube 262, meanwhile, the sampling rotary shaft 23 drives the sampling gear 27 to rotate, the sampling gear 27 rotates to drive the linkage rack 31 to move, the linkage rack 31 moves to drive the vibration raised strips 32 to move, the vibration raised strips 32 move to drive the arc-shaped protruding blocks 321 to move, the arc-shaped protruding blocks 321 move to move up and down through the trapezoid grooves 342 to drive the vibration blocks 34 to move up and down, and when the sampling bottle 261 moves to the extrusion supporting block 112 and supports the extrusion supporting block 112, the extrusion supporting block 112 supports the bottom of the extrusion bottle 261, so that the bacterial liquid in the sampling tube 262 is extruded and dropped on the glass slide 36, and the sampling motor 22 is stopped;

step two: starting the sampling motor 22 to reversely rotate, reversely rotating the sampling rotating shaft 23 and the sampling gear 27, driving the linkage rack 31 to move towards the direction close to the placing plate 14 by the sampling gear 27, driving the vibration raised strips 32 to move towards the direction close to the placing plate 14 by the linkage rack 31, repeatedly vibrating the vibration blocks 34 up and down by the movement of the vibration raised strips 32, and driving the bearing table 35 to vibrate by the vibration blocks 34 to enable the glass slide 36 to vibrate, so that bacterial colonies can be more uniformly dispersed on the glass slide 36, and then stopping the sampling motor 22;

Step three: starting a moving motor 43, wherein the moving motor 43 drives a moving threaded rod 46 to rotate, two guide rods 45 limit a moving block 47, the moving threaded rod 46 rotates to drive the moving block 47 to move, the moving block 47 moves to drive a bearing table 35 to move, so that a glass slide 36 moves, when the moving block 47 and the bearing table 35 move into a drying box 51, the moving motor 43 is stopped, and the drying box 51 heats and dries bacterial liquid on the glass slide 36;

step four: after drying, the moving motor 43 is continuously started, the moving block 47 continuously drives the carrying table 35 and the glass slide 36 to move, when the glass slide 36 moves below the dyeing device 54, the moving motor 43 is stopped, the sensor 55 senses that the glass slide 36 stays, and the dyeing device 54 drops the dyeing agent onto the glass slide 36 to dye colonies on the glass slide 36;

step five: after the staining agent is dripped into, the moving motor 43 is continuously started, the moving block 47 is enabled to move continuously, the moving block 47 drives the slide glass 36 on the carrying table 35 to stop moving the motor 43 when moving to the position right below the detection probe 65, the detection cylinder 63 is started, the detection cylinder 63 drives the detection device 64 to move downwards, the detection device 64 moves downwards to drive the rotating plate 66 and the translation movable plate 67 to move downwards, after the positioning clamping blocks 68 enter and abut against the corresponding supporting abutting grooves 681, the two translation movable plates 67 drive the two smearing pull plates 69 to move in the directions away from each other, the staining agent is smeared uniformly on two sides, the two translation movable plates 67 are separated from the position above the slide glass 36 after being separated from each other, the detection probe 65 moves downwards to the position above the slide glass 36, and the detection device 64 detects and analyzes the colony above the slide glass 36 through the detection probe 65.

In one embodiment, when an operator needs to detect pathogenic microorganisms in a bacterial liquid, the operator opens the rotary door 15, pours the bacterial liquid into the placing bottle 141, then closes the rotary door 15, isolates external dust and other pollutants through the protective housing 12, ensures a dust-free and pollution-free environment in the detection process, the sampling bottle 261 has a heavy weight, the gravity center is positioned below the connecting shaft 25, so as to ensure that the opening directions of the sampling bottle 261 and the sampling tube 262 are always vertically downward, in an initial state, the sampling holding block 142 is held at the bottom of the sampling bottle 261, the held part of the sampling bottle 261 is extruded to be in a compressed state, after the bacterial liquid is poured into the placing bottle 141, the bottom of the sampling tube 262 is positioned in the bacterial liquid of the placing bottle 141, the sampling motor 22 is started, the sampling motor 22 drives the sampling rotating shaft 23 to rotate, the sampling rotating shaft 23 drives the sampling rotating arm 24 to overturn, the other end of the sampling rotating arm 24 turns over to drive the connecting shaft 25 to move, the connecting shaft 25 moves gradually away from the sampling supporting block 142, the connecting shaft 25 drives the sampling bottle 261 to move away from the sampling supporting block 142, so that the sampling supporting block 142 cannot support the sampling bottle 261, the compressed part of the bottom of the sampling bottle 261 has elasticity to recover, bacterial liquid at the bottom is sucked into the sampling tube 262, the sampling rotating shaft 23 rotates to enable the sampling bottle 261 to move away from the sampling supporting block 142 through the sampling rotating arm 24 and the connecting shaft 25, the sampling bottle 261 moves to the extrusion supporting block 112 and supports against the extrusion supporting block 112, the extrusion supporting block 112 supports against the bottom of the extrusion sampling bottle 261, the bottom of the sampling bottle 261 is compressed and deformed again, bacterial liquid in the sampling tube 262 is extruded and dripped on the glass slide 36, the sampling motor 22 is stopped, the bacterial liquid is sampled when the sampling motor 22 is stopped, due to the inertia effect, the bacterial liquid in the sampling tube 262 has a downward moving trend, so that the bacterial liquid drops on the glass slide 36 with larger force, and the bacterial liquid dropped on the glass slide 36 can be outwards diffused, so that the area of the bacterial liquid on the glass slide 36 is increased, the diffusion area of the bacterial liquid is increased, the bacterial liquid is prevented from being accumulated on the surface of the glass slide 36 to form a bead-shaped, a relatively uniform thin layer is formed on the glass slide 36, detection and analysis are better performed, the accuracy and the precision in detection are improved, and the practicability and the detection efficiency are improved.

When the sampling bottle 261 moves away from the sampling holding block 142 towards the direction of extruding the holding block 112, the sampling rotating shaft 23 rotates to drive the sampling gear 27 to rotate, the sampling gear 27 rotates to drive the linkage rack 31 to move, the linkage rack 31 moves to drive the vibration raised strips 32 to move, the vibration raised strips 32 move to drive the arc-shaped protruding blocks 321 to move, the arc-shaped protruding blocks 321 can move to drive the vibration block 34 to move up and down through the trapezoid grooves 342, when the arc-shaped protruding blocks 321 come out from the trapezoid grooves 342, the trapezoid grooves 342 can be held against one side wall of the linkage rack 31 and move, the vibration block 34 moves upwards, the two sides of the vibration block 34 are provided with the lower pressing blocks 341, the upward movement of the vibration block 34 drives the lower pressing blocks 341 to move upwards, the upward movement of the lower pressing blocks 341 compresses the lower pressing springs 332, when the arc-shaped protruding blocks 321 enter the trapezoid grooves 342 from the bottoms of the vibration block 34, the vibration block 34 makes the vibration block 34 move downwards to reset through self gravity and the elasticity of the downward pressing spring 332, the upper and lower vibration of the vibration block 34 is realized through the cooperation of a plurality of arc-shaped protruding blocks 321 and a plurality of trapezoid grooves 342, an arc-shaped groove is formed in one side wall of the glass slide 36, the arc-shaped groove can be beneficial to the placement and the taking out of the glass slide 36, and the arc-shaped groove can prevent the situation that the bottom air cannot be smoothly discharged due to the vibration when the side wall of the glass slide 36 and the side wall of the mounting groove 351 are mutually adhered when the vibration block 34 drives the glass slide 36 to vibrate, the glass slide 36 is cracked, the arc-shaped groove on the glass slide 36 can smoothly discharge the bottom air, the occurrence of the damage of the glass slide 36 is reduced, the upper and lower vibration of the vibration block 34 can vibrate up and down at the movable bearing table 35, the upper and lower vibration of the bearing table 35 can drive the glass slide 36 to vibrate up, the up and down vibration of the slide 36 can cause the upper surface of the slide 36 to be positively or negatively charged, and microorganisms and other components in the bacterial liquid also carry positive or negative charges, and they will generate electrostatic attraction when falling on the slide 36 with opposite charges, so as to increase microscopic friction, and make the colonies in the bacterial liquid more easily adhere and fix on the slide 36.

Similarly, after the bacterial liquid drops fall on the glass slide 36 and the sampling motor 22 is stopped, the sampling motor 22 is reversely driven to reversely rotate the output shaft of the sampling motor 22, so that the sampling rotating shaft 23 and the sampling gear 27 reversely rotate, the sampling gear 27 drives the linkage rack 31 to move towards the direction close to the placing plate 14, the linkage rack 31 drives the vibration raised strips 32 to move towards the direction close to the placing plate 14, the vibration raised strips 32 move to drive the vibration blocks 34 to repeatedly vibrate up and down, the glass slide 36 vibrates, the surface tension among the small bacterial liquid drops is reduced through vibration, the bacterial liquid is ensured to be evenly distributed on the surface of the glass slide 36, bacterial liquid can be better dispersed on the glass slide 36, the vibration glass slide 36 can also help the bacterial liquid to better permeate into holes of the glass slide 36, so that the bacterial liquid on the glass slide 36 can be uniformly distributed and fixed on the glass slide 36, and experimental precision and reliability are improved.

After the sampling motor 22 reversely rotates to reset the sampling bottle 261, the sampling motor 22 is stopped, the moving motor 43 is started, the moving motor 43 drives the moving threaded rod 46 to rotate, and the two guide rods 45 limit the moving block 47, the moving threaded rod 46 rotates to drive the moving block 47 to move, two linkage clamping grooves 471 are formed in the top of the moving block 47, and the bearing table 35 is clamped in the two linkage clamping grooves 471 through the two linkage clamping blocks 359, so that the bearing table 35 can be driven to move when the moving block 47 moves, and the glass slide 36 is driven to move, when the moving block 47 and the bearing table 35 move into the drying box 51, the moving motor 43 is stopped, the drying box 51 heats and dries bacterial liquid on the glass slide 36, after drying is completed, the moving block 47 continues to drive the bearing table 35 and the glass slide 36 to move, when the glass slide 36 moves to the lower part of the dyeing machine 54, the moving motor 43 is stopped, the dyeing machine 54 drops into the glass slide 36 for a second, the dyeing agent is dripped into the glass slide 36, the dyeing agent is prevented from being damaged, the bacterial liquid is well distributed in the drying process, the bacterial liquid is prevented from being damaged, the bacterial liquid is well in the drying process, and the bacterial liquid is prevented from being polluted, and the bacterial liquid is well adhered to the glass slide 36 is well, and the bacterial liquid is well dried, and the bacterial liquid is prevented from being polluted.

When the dyeing agent is dripped, the moving motor 43 is continuously started to enable the moving block 47 to move continuously, the moving block 47 drives the slide glass 36 on the bearing table 35 to move right below the detection probe 65, the moving motor 43 is stopped, the detection cylinder 63 is started, the detection cylinder 63 drives the detection device 64 to move downwards, the detection device 64 moves downwards to drive the rotating plate 66 to move downwards, the rotating plate 66 moves downwards to drive the two translation movable plates 67 to move downwards, one ends of the two translation movable plates 67 are mutually propped, the rotating plate 66 and the translation movable plates 67 are mutually connected through torsion springs, the two translation movable plates 67 are mutually parallel in a natural state, when the rotating plate 66 drives the two translation movable plates 67 to move downwards, the translation movable plates 67 drive the positioning clamping blocks 68 to move downwards to enable the positioning clamping blocks 68 to enter and prop against the corresponding supporting propping grooves 681, the side wall at the bottom of the positioning clamping block 68 is propped against the side wall of the supporting propping groove 681, the bottom of the smearing pull piece 69 contacts with the coloring agent on the glass slide 36, the detection device 64 continues to move downwards, so that the bottoms of the two rotating plates 66 turn over and move in the direction away from each other, the bottoms of the two rotating plates 66 drive the two translation movable plates 67 to move in the direction away from each other, the mutual distance of the two translation movable plates 67 can drive the two smearing pull pieces 69 to move in the direction away from each other, the coloring agent is smeared uniformly on two sides, so that the bacterial colony is fully dyed, when the distance between the two translation movable plates 67 is further and further, one ends of the two translation movable plates 67 close to each other are slightly upstairs, the damage to the bacterial colony and the upper surface of the glass slide 36 when the smearing pull piece 69 scrapes the coloring agent is prevented, the two translation movable plates 67 are far away from each other and then are away from the upper side of the glass slide 36, the detection probe 65 moves downwards to the upper side of the glass slide 36 to detect the bacterial colony on the glass slide 36, and the detection device 64 detects and analyzes the bacterial colony above the glass slide 36 through the detection probe 65, so that pathogenic microorganisms in the bacterial colony are detected and analyzed, and the detection accuracy and the detection practicability are improved.

During installation, the working base 11 is fixedly installed on the ground, the protective shell 12 is fixedly installed at the top of the working base 11, the placing support plate 13 is fixedly installed at the top of one end of the working base 11, the placing plate 14 is horizontally and fixedly installed at the top of the placing support plate 13, the rotating door 15 is rotatably installed on the side wall of the opening groove 121, the sampling support plate 21 is fixedly installed at the top of the working base 11, the sampling motor 22 is fixedly installed on one side wall of the sampling support plate 21, the sampling rotating shaft 23 is fixedly installed on the output shaft of the sampling motor 22, one end of the sampling rotating arm 24 is fixedly installed in the middle of the sampling rotating shaft 23, one end of the connecting shaft 25 is rotatably installed on the other end of the sampling rotating arm 24, sampling piece 26 fixed mounting is on the other end of connecting axle 25, sampling gear 27 fixed mounting is kept away from the one end tip of sampling backup pad 21 at sampling pivot 23, the top fixed mounting of sampling bottle 261 a lateral wall is on the tip of sampling pivot 23, sampling pipe 262 fixed mounting is in the bottom of sampling bottle 261, linkage rack 31 slidable mounting is at the top of work base station 11, vibration sand grip 32 fixed mounting is kept away from the bottom of placing plate 14 one end at linkage rack 31, the top of vibration sand grip 32 upwards protruding is equipped with a plurality of arc lug 321, two equal fixed mounting of vibration backup pad 33 are at the top of work base station 11, two lower briquetting 341 are fixed mounting in both sides of vibrating piece 34, plummer 35 wears to locate in the convex groove 343.

The movable connecting plate 41 is fixedly arranged on the side wall of the two vibration supporting plates 33 facing the sampling gear 27, the motor mounting table 42 is fixedly arranged on the top of one end of the working base table 11 far away from the placing plate 14, the movable motor 43 is fixedly arranged on the top of the motor mounting table 42, the two guide supporting plates 44 are fixedly arranged on the top of the motor mounting table 42, one ends of the two guide rods 45 are respectively fixedly arranged on the side walls of the two guide supporting plates 44, one end of the movable threaded rod 46 is fixedly arranged on the output shaft of the movable motor 43, the movable block 47 is in threaded sleeve on the movable threaded rod 46, the drying box 51 is fixedly arranged on the working base table 11, one end of the dyeing supporting plate 52 is fixedly arranged on the working base table 11, the dyeing connecting plate 53 is fixedly arranged on the extending end of the dyeing supporting plate 52, the dyeing device 54 is fixedly arranged on the bottom of the dyeing connecting plate 53, the sensor 55 is fixedly arranged at the bottom of the dyeing connecting plate 53 and is positioned at one side of the dyeing device 54 far away from the drying box body 51, the four detection supporting legs 61 are fixedly arranged at the top of the working base 11, the detection supporting table 62 is fixedly arranged at the top of the four detection supporting legs 61, the detection cylinder 63 is fixedly arranged at the top of the detection supporting table 62, the detection device 64 is fixedly arranged at the output end of the detection cylinder 63, the detection probe 65 is fixedly arranged on the detection device 64, the two rotating plates 66 are respectively rotatably arranged at the bottoms of the two side walls of the detection probe 65, one ends of the two translation movable plates 67 far away from each other are respectively rotatably arranged at the bottoms of the two rotating plates 66 through torsion springs, the two positioning clamping blocks 68 are respectively and slidably clamped in the translation sliding grooves 671 of the two translation movable plates 67, the bottoms of the positioning clamping blocks 68 can enter and abut against the bottoms and the side walls of the supporting and holding grooves 681, the top parts of the two smearing pull pieces 69 are respectively fixedly arranged at the bottoms of one ends of the two translation movable plates 67, which are close to each other.

The beneficial effects are that:

1. by utilizing the inertia effect, the bacterial liquid in the sampling tube 262 has a downward moving trend, so that the bacterial liquid is dropped on the glass slide 36 with larger force, and the bacterial liquid dropped on the glass slide 36 can be outwards diffused, so that the area of the bacterial liquid on the glass slide 36 is increased, the diffusion area of the bacterial liquid is increased, the bacterial liquid is prevented from being piled up on the surface of the glass slide 36 to form a bead-shaped, a relatively uniform thin layer is formed on the glass slide 36, detection and analysis are better performed, the accuracy and the precision in detection are improved, and the practicability and the detection efficiency are improved.

2. The up-and-down vibration of the vibration block 34 can vibrate up and down on the movable bearing table 35, the up-and-down vibration of the bearing table 35 can drive the slide 36 to vibrate up, the up-and-down vibration of the slide 36 can lead the upper surface of the slide 36 to carry positive or negative charges, microorganisms and other components in bacterial liquid also carry positive or negative charges, and electrostatic attraction force can be generated when the microorganisms and other components fall on the slide 36 with opposite charges, so that microscopic friction force is improved, and bacterial colonies in bacterial liquid are more easily attached and fixed on the slide 36;

the surface tension between the small liquid drops of the bacterial liquid is reduced through vibration, the bacterial liquid is ensured to be evenly distributed on the surface of the glass slide 36, so that bacterial colonies can be more evenly dispersed on the glass slide 36, the vibration glass slide 36 can also help the bacterial liquid to better permeate into holes of the glass slide 36, thereby being beneficial to the fixation and collection of bacterial colonies, and the vibration of the glass slide 36 can promote the bacterial liquid on the glass slide 36 to be evenly distributed and fixed on the glass slide 36, so that the experimental precision and reliability are improved.

3. Bacterial liquid on the glass slide 36 is dried to better fix and preserve bacterial colonies, so that bacterial colonies are prevented from being damaged and shifted before or during dyeing, adhesion force between the bacterial colonies and the glass slide 36 can be increased during drying, the dyeing agent is more uniformly distributed on the glass slide 36, phenomena of twisting, drifting and the like caused by liquid drops during dyeing can be avoided, and accuracy and good dyeing effect are ensured.

4. Can smear the colorant to both sides evenly, and prevent to smear the upper surface that drags piece 69 damaged bacterial colony and slide 36 when scraping the colorant, two translation fly leaves 67 after keeping away from each other and will leave the top of slide 36, and detection probe 65 moves down to the top of slide 36 and detects the bacterial colony on the slide 36, and detection device 64 detects the analysis through detection probe 65 to the bacterial colony of slide 36 top, thereby realize detecting and analyzing the pathogenic microorganism in the bacterial colony, improved accuracy and the practicality of detection.

All possible combinations of the technical features in the above embodiments are described, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above embodiments represent only a few embodiments of the present invention, which are described in more detail and are not to be construed as limiting the scope of the present invention. It should be noted that numerous variations and modifications could be made to the person skilled in the art without departing from the spirit of the invention, which would fall within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims

1. The detection method of pathogenic microorganisms is characterized by applying a detection device of pathogenic microorganisms, wherein the detection device of pathogenic microorganisms comprises a protection component (10), a sampling component (20), a vibration component (30), a moving component (40), a drying and dyeing component (50) and a detection component (60), the protection component (10) comprises a working base (11), a protection shell (12), a placing support plate (13), a placing plate (14) and a rotating door (15), the working base (11) is fixedly arranged on the ground, the protection shell (12) is fixedly arranged on the top of the working base (11), an opening groove (121) is formed in one side wall end of the protection shell (12), the placing support plate (13) is fixedly arranged on the top of one end of the working base (11), the placing plate (14) is horizontally and fixedly arranged on the top of the placing support plate (13), the rotating door (15) is rotatably arranged on the side wall of the opening groove (121), the sampling component (20) is fixedly arranged on the top of the working base (11) and is positioned on one side of the placing plate (14), the vibration component (30) is arranged on the top of the working base (11) and is positioned on one side of the moving component (40), the drying and dyeing assembly (50) is fixedly arranged at the top of the working base (11) and is positioned at one side of the vibrating assembly (30) away from the placing plate (14), the detection assembly (60) is fixedly arranged at the top of the working base (11) and is positioned at one side of the drying and dyeing assembly (50) away from the vibrating assembly (30), a circular placing groove is formed in the top of the placing plate (14), a placing bottle (141) is placed on the placing groove, an L-shaped sampling supporting block (142) is fixedly arranged at one end of the placing plate (14), one end of the sampling supporting block (142) is fixedly arranged on the placing plate (14), the other end extends above the placing bottle (141), an L-shaped supporting plate (111) is fixedly arranged at the top of the working base (11), an L-shaped extruding supporting block (112) is fixedly arranged at the top of the supporting plate (111), one end of the extruding supporting block (112) is fixedly arranged on the working base (11), the other end extends towards the rotating door (15), the sampling assembly (20) comprises a sampling motor (21), a supporting plate (22), a rotating arm (24), a sampling shaft (24), a sampling gear (27) and a sampling shaft (27) are fixedly arranged at the top of the working base (11), the sampling motor (22) is fixedly arranged on one side wall of the sampling supporting plate (21), an output shaft of the sampling motor (22) penetrates through the sampling supporting plate (21), the sampling rotating shaft (23) is fixedly arranged on the output shaft of the sampling motor (22), one end of the sampling rotating arm (24) is fixedly arranged in the middle of the sampling rotating shaft (23), one end of the connecting shaft (25) is rotatably arranged at the other end of the sampling rotating arm (24), the sampling piece (26) is fixedly arranged at the other end of the connecting shaft (25), the sampling gear (27) is fixedly arranged at the end part of the sampling rotating shaft (23) far away from the sampling supporting plate (21), the sampling piece (26) comprises a sampling bottle (261) and a sampling tube (262), the top of one side wall of the sampling bottle (261) is fixedly arranged on the end part of a sampling rotating shaft (23), the sampling tube (262) is fixedly arranged at the bottom of the sampling bottle (261), the sampling bottle (261) is communicated with the inside of the sampling tube (262), the side wall of the bottom of the sampling bottle (261) is made of rubber, the bottom of the sampling bottle (261) can be pressed and deformed by the sampling supporting block (142) and the extruding supporting block (112), the top of the workbench (11) is convexly provided with two rack limiting plates (113), the two rack limiting plates (113) are both positioned below the sampling gear (27), the vibration subassembly (30) comprises a linkage rack (31), a vibration raised strip (32), two vibration supporting plates (33), a vibration block (34) and a bearing table (35), the linkage rack (31) is slidably mounted at the top of the working base table (11) and is positioned between two rack limiting plates (113), the top of the linkage rack (31) is meshed with a sampling gear (27) mutually, the vibration raised strip (32) is fixedly mounted at the bottom of one end of the linkage rack (31) far away from the placing plate (14), the vibration raised strip (32) can slide at the top of the working base table (11), a plurality of arc-shaped protruding blocks (321) are upwardly protruding from the top of the vibration raised strip (32), the two vibration supporting plates (33) are fixedly mounted at the top of the working base table (11) and are respectively positioned at the two sides of the vibration raised strip (32), spring grooves (331) are respectively formed in side walls facing each other, vibration pressing springs (332) are fixedly mounted at the top of the spring grooves (331), two pressing blocks (341) are fixedly mounted at the two sides of the vibration block (34) and are connected with the bottoms of the two pressing blocks (341) respectively between the two vibration supporting plates (33), a plurality of trapezoid grooves (342) are formed in the bottom of the vibrating block (34), the trapezoid grooves (342) and the arc-shaped convex blocks (321) are arranged in parallel, part of the arc-shaped convex blocks (321) are propped against the side wall of the trapezoid grooves (342), the convex grooves (343) are formed in the vibrating block (34) towards the side wall of the sampling gear (27) in a penetrating mode, the bearing table (35) is arranged in the convex grooves (343) in a penetrating mode, square mounting grooves (351) are formed in the top of the bearing table (35), glass slides (36) are mounted at the bottoms of the mounting grooves (351), two Z-shaped grooves (353) are symmetrically formed in the mounting grooves (351) towards the side wall of two vibrating support plates (33), Z-shaped clamping blocks (354) are slidably mounted in the two Z-shaped grooves (353), a reset groove (355) is formed in one side wall of the two Z-shaped grooves (353) which faces away from each other, a reset spring (356) is fixedly mounted in the reset groove (355), one end of the reset spring (356) is fixedly mounted on the side wall of the mounting groove (351), the other end of the reset spring (356) is fixedly mounted in the middle of the side wall of the mounting table (354), the middle of the Z-shaped clamping block (354) is mounted on the side wall, the two Z-shaped clamping blocks are arranged close to the two sides of the two vibrating support plates (35) respectively, and the two bar-shaped grooves (357) are communicated with the corresponding Z-shaped grooves (353) respectively, the moving assembly (40) comprises a moving connecting plate (41), a motor mounting table (42), a moving motor (43), two guide supporting plates (44), two guide rods (45), a moving threaded rod (46) and a moving block (47), the moving connecting plate (41) is fixedly arranged on the side wall of the two vibrating supporting plates (33) facing the sampling gear (27), the motor mounting table (42) is fixedly arranged on the top of one end of the working base table (11) far away from the placing plate (14), the moving motor (43) is fixedly arranged on the top of the motor mounting table (42), the two guide supporting plates (44) are fixedly arranged on the top of the motor mounting table (42) and are positioned on two sides of the output shaft of the moving motor (43), one end of the two guide rods (45) is fixedly arranged on the side wall of the two guide supporting plates (44), the other end of the moving threaded rod (46) is fixedly arranged on the side wall of the moving connecting plate (41), one end of the moving threaded rod (46) is fixedly arranged on the output shaft of the moving motor (43), the other end of the moving threaded rod (41) is rotatably arranged on the side wall of the moving connecting plate (47), the bottom of the bearing table (35) is provided with a movable groove (358), the bottom of the bearing table (35) is downwards provided with two linkage clamping blocks (359) in a protruding way, the moving block (47) is positioned in the movable groove (358), the top of the moving block (47) is provided with two linkage clamping grooves (471), the two linkage clamping blocks (359) are respectively inserted into the two linkage clamping grooves (471), the drying dyeing component (50) comprises a drying box body (51), a dyeing supporting plate (52), a dyeing connecting plate (53), a dyeing device (54) and an inductor (55), the drying box body (51) is fixedly arranged on the working base table (11) and is positioned on one side of a vibration raised strip (32) far away from the linkage rack (31), the middle part of the drying box body (51) is provided with a through groove, one end of the dyeing supporting plate (52) is fixedly arranged on the working base table (11) and is positioned on one side of the drying box body far away from the vibration raised strip (32), the other end extends towards the upper side of the vibration raised strip (46), the dyeing connecting plate (53) is fixedly arranged on the dyeing device (53) far from the dyeing end of the dyeing connecting plate (52) and is fixedly arranged on the dyeing connecting plate (53) which is fixedly arranged on the bottom of the dyeing connecting plate (53), and is located one side that the dying device (54) kept away from stoving box (51), the one end that two Z shape fixture blocks (354) kept away from each other is formed with and presss from both sides tight inclined plane (350), detection subassembly (60) are including four detection supporting legs (61), detect supporting bench (62), detect cylinder (63), detection device (64), detect probe (65), two rotation plates (66), two translation fly leaf (67), two positioning fixture blocks (68) and two scribble drag leaf (69), four detection supporting legs (61) fixed mounting are in the top of workstation (11), detect supporting bench (62) fixed mounting are in the top of four detection supporting legs (61), and be located the top of two guide bars (45) and removal threaded rod (46), detect cylinder (63) fixed mounting are in the top of detecting supporting bench (62), and the output of detecting cylinder (63) runs through detection supporting bench (62) and extends down, detection device (64) fixed mounting is on the output of detecting cylinder (63), detect probe (65) fixed mounting is on detection device (64), two rotation plates (66) are installed in the top of detecting device (64), two rotation plates (66) respectively and are installed in the threaded rod (46) and are perpendicular to the bottom of moving threaded rod (46), one ends, far away from each other, of the two translation movable plates (67) are respectively installed at the bottoms of the two rotation plates (66) in a rotating mode through torsion springs, translation sliding grooves (671) are formed in the bottoms of the translation movable plates (67), two positioning clamping blocks (68) are respectively and slidably clamped in the translation sliding grooves (671) of the two translation movable plates (67), transverse translation springs (672) are fixedly installed in the translation sliding grooves (671), supporting and supporting grooves (681) are further formed in the bottoms of the strip-shaped grooves (357), the bottoms of the positioning clamping blocks (68) can enter and are supported on the bottoms and the side walls of the supporting and supporting grooves (681), the tops of the two smearing and dragging pieces (69) are respectively and fixedly installed at the bottoms, close to one ends of the two translation movable plates (67), respectively, and the detection method comprises the following steps:

Step one: an operator opens a rotating door (15), bacterial liquid is poured into a placing bottle (141), then the rotating door (15) is closed, a sampling motor (22) is started, a sampling rotating shaft (23) is driven by the sampling motor (22) to rotate so that a sampling bottle (261) is far away from a sampling supporting block (142), bacterial liquid at the bottom is sucked into a sampling tube (262), meanwhile, the sampling rotating shaft (23) drives a sampling gear (27) to rotate, the sampling gear (27) rotates to drive a linkage rack (31) to move, the linkage rack (31) moves to drive a vibration raised strip (32) to move, the vibration raised strip (32) moves to drive a plurality of arc-shaped protruding blocks (321) to move, the arc-shaped protruding blocks (321) move to drive a vibration block (34) to move up and down through a trapezoid groove (342), when the sampling bottle (261) moves to the extrusion supporting block (112) and supports the extrusion supporting block (112), the extrusion supporting block (112) supports the bottom of the extrusion supporting block (261), and accordingly bacterial liquid in the sampling bottle (262) is extruded by the sampling motor (36) and drops down;

Step two: starting a sampling motor (22) to reversely rotate, enabling a sampling rotating shaft (23) and a sampling gear (27) to reversely rotate, enabling the sampling gear (27) to drive a linkage rack (31) to move towards a direction close to a placing plate (14), enabling a vibration convex strip (32) to move towards a direction close to the placing plate (14), enabling a vibration convex strip (32) to move to drive a vibration block (34) to repeatedly vibrate up and down, enabling a bearing table (35) to vibrate by the vibration block (34), enabling a glass slide (36) to vibrate, enabling bacterial colonies to be more uniformly dispersed on the glass slide (36), and then stopping the sampling motor (22);

step three: starting a moving motor (43), wherein the moving motor (43) drives a moving threaded rod (46) to rotate, two guide rods (45) limit a moving block (47), the moving threaded rod (46) rotates to drive the moving block (47) to move, the moving block (47) moves to drive a bearing table (35) to move, so that a glass slide (36) moves, when the moving block (47) and the bearing table (35) move into a drying box body (51), the moving motor (43) is stopped, and the drying box body (51) heats and dries bacterial liquid on the glass slide (36);

Step four: after drying, continuously starting a moving motor (43), continuously driving a bearing table (35) and a glass slide (36) by a moving block (47), stopping moving the motor (43) when the glass slide (36) moves below a dyeing device (54), and dripping a dyeing agent onto the glass slide (36) by the dyeing device (54) to dye colonies on the glass slide (36) after the sensor (55) senses that the glass slide (36) stays;

step five: after the staining agent is dripped, the moving motor (43) is continuously started, the moving block (47) is enabled to move continuously, the moving block (47) drives the slide glass (36) on the bearing table (35) to move to the position right below the detection probe (65), the moving motor (43) is stopped, the detection cylinder (63) is started, the detection cylinder (63) drives the detection device (64) to move downwards, the detection device (64) moves downwards to drive the rotating plate (66) and the translation movable plate (67) to move downwards, after the positioning clamping block (68) enters and abuts against the corresponding supporting and abutting groove (681), the two translation movable plates (67) drive the two smearing drags (69) to move in the direction away from each other, the staining agent is smeared evenly to two sides, the two translation movable plates (67) are kept away from each other and then leave the position above the slide glass (36), the detection probe (65) moves downwards to the position above the slide glass (36) to detect colonies above the slide glass (36), and the detection device (64) detects colonies above the slide glass (36) through the detection probe (65) to analyze colonies.