CN116558919A - Slicing manufacturing method suitable for identifying rosewood - Google Patents
Slicing manufacturing method suitable for identifying rosewood Download PDFInfo
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- CN116558919A CN116558919A CN202310493231.XA CN202310493231A CN116558919A CN 116558919 A CN116558919 A CN 116558919A CN 202310493231 A CN202310493231 A CN 202310493231A CN 116558919 A CN116558919 A CN 116558919A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 27
- 238000002791 soaking Methods 0.000 claims abstract description 25
- 238000009835 boiling Methods 0.000 claims abstract description 16
- 239000002352 surface water Substances 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 87
- 244000086363 Pterocarpus indicus Species 0.000 claims description 32
- 235000019441 ethanol Nutrition 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 15
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 238000004043 dyeing Methods 0.000 claims description 10
- 239000011521 glass Substances 0.000 claims description 10
- 239000008096 xylene Substances 0.000 claims description 10
- 239000010634 clove oil Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 239000006059 cover glass Substances 0.000 claims description 5
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000000975 dye Substances 0.000 claims description 5
- 239000001046 green dye Substances 0.000 claims description 5
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 230000007935 neutral effect Effects 0.000 claims description 5
- 238000000643 oven drying Methods 0.000 claims description 5
- 239000001044 red dye Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- 239000012188 paraffin wax Substances 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 239000002023 wood Substances 0.000 description 20
- 241000220324 Pyrus Species 0.000 description 19
- 235000014443 Pyrus communis Nutrition 0.000 description 15
- 239000000463 material Substances 0.000 description 7
- 235000004789 Rosa xanthina Nutrition 0.000 description 6
- 241000109329 Rosa xanthina Species 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 5
- 235000021017 pears Nutrition 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 241000522195 Dalbergia Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 241000657528 Dalbergia odorifera Species 0.000 description 1
- 244000179970 Monarda didyma Species 0.000 description 1
- 235000010672 Monarda didyma Nutrition 0.000 description 1
- 240000008976 Pterocarpus marsupium Species 0.000 description 1
- 235000010453 Pterocarpus marsupium Nutrition 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 206010051373 Wound haemorrhage Diseases 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011162 core material Substances 0.000 description 1
- 238000011840 criminal investigation Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- 230000009972 noncorrosive effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
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- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a slicing manufacturing method suitable for identifying rosewood. The manufacturing method comprises the steps of softening, slicing and tabletting, wherein the specific steps of softening are as follows: soaking the sample in water overnight, boiling in water for 0.8-1 h, sucking the surface water stain by using filter paper after boiling in water, and storing in glycerol-absolute ethanol for later use. The method can effectively improve the softening effect, obviously shorten the water boiling time, facilitate slicing, and ensure that the prepared slicing effect is better and is more beneficial to observing the microstructure.
Description
Technical Field
The invention relates to the field of plant slice manufacturing, in particular to a slice manufacturing method suitable for identifying rosewood.
Background
The rosewood (Dalbergia odorifera) is also called rosewood, which belongs to the genus Dalbergia (Dalbergia) plant of the family Papilionaceae, and is also called rosewood, pterocarpus marsupium, bergamot, rosewood, and yellow pear. The flower pears are wide in application, not only have high wood and medicinal value, but also are drought-resistant and barren-resistant, and are valuable rural tree species which are worthy of popularization. The flower pear is a special precious tree species in China. The flower pears are mainly produced in the middle and south of Hainan, in the middle sea, in the mountain slope and forest margin. The wood is excellent in quality, the side materials are light yellow, the quality is slightly loose, the core material is reddish brown, the weight is high, the texture is compact, and the wood is a good furniture material of the upper class; has fragrance, and can be used as perfume; the root heart wood is named rosewood and is used for medicine. Is a good analgesic and also can treat incised wound hemorrhage.
The height of the roses is 15-20 m, the chest diameter can reach 80cm, the heartwood is reddish brown or purplish red brown, the roses are changed into dark red after a long time, and the roses often contain dark brown or black stripes, have luster, fragrance, strong pungent smell of new sections, slight fragrance after a long time, fine structure, staggered wood textures, natural forming and attractive patterns. Furniture made of the rosewood is concise, clear, rich and magnificent, deep, elegant, noble, durable and non-corrosive for century. The rosewood furniture can also emit the fragrant smell for a long time, and has the expression of refreshing and avoiding pathogenic factors. Along with the violent rise of the price of the wood, the market of the flower pear seedlings is also unprecedented. In order to meet the market demand, in recent years, a great deal of manpower, material resources and financial resources are invested in the seedling raising work of the roses by forestry departments, and at present, the seedling raising and cutting seedling raising technology of the roses seeds is mature and is widely popularized. However, as the heartwood of the flower pears is slowly formed, the growth period is long, the flower pears can be grown in tens or even hundreds of years, and the growing demands of the market cannot be met at all in a short time. In recent years, due to the special shortage of large-diameter materials, the price of the roses is unprecedented and is even counted by jin, and each jin reaches thousands yuan. In order to obtain high profit, lawless persons often appear frequently with fake ones, and the market order is seriously disturbed. With the expansion of the pear market, the identification of pear wood has also been developed as a profession. Wood identification and authentication refers to the process of authenticating wood to genus, species or species by personnel engaged in wood production, processing, circulation and quality inspection activities through macroscopic or microscopic features of wood, which is the most basic task of correctly recognizing and scientifically and reasonably utilizing wood. Meanwhile, because the difference of the structural characteristics of the same wood is relatively small, the wood can play an irreplaceable important role in scientific archaeology and criminal investigation and arbitration inspection at times. The flower pear is taken as a precious wood, the value and the material of the flower pear are not comparable to those of common wood, so that the flower pear is very numerous in counterfeit products and inferior products in the market, the current flower pear wood identification is mainly judged by the sense and experience of an identifier, the error is large, the reliability is low, the current flower pear wood has extremely high price, and the economic loss is large once misjudgment is carried out. In order to accurately discriminate the authenticity of the wood, maintain the market, and develop the research of the rosewood identification technology in depth, the method has important scientific value and practical guiding significance.
The rosewood often presents a tricky pattern, is rich in morphology, such as a moire pattern, a mountain peak pagoda pattern, a sea water moire pattern and the like, and also has a special ghost face. The texture is an important factor for forming commodity value, and the slice texture can be clearly observed, so that the method has important significance.
The sample softening process for making the cut rosewood slices generally adopts a water boiling method, and the water boiling method generally needs to be boiled for 3 hours to days according to the wood material. The boiling state needs to be kept for a long time, high requirements are imposed on personnel and equipment, and certain dangers are caused in operation.
Disclosure of Invention
In view of the above, the present invention provides a slicing method suitable for identifying rosewood, which solves the above problems.
The technical scheme of the invention is realized as follows:
the preparation method comprises the steps of softening, slicing and tabletting, wherein the specific steps of softening are as follows: soaking the sample in clear water overnight, boiling in water for 0.8-1 h, cooling, sucking the surface water stain with filter paper, and storing in glycerol-absolute ethanol for later use.
Further, the pear sample was sampled over 30 years of heartwood.
Further, the volume ratio of glycerin to absolute ethanol in the glycerin-absolute ethanol is 1:2.
further, the slicing machine is a flat-pushing type slicing machine or a paraffin slicing machine.
Further, the tabletting comprises the steps of dyeing, dehydrating, transparentizing and sealing.
Further, the specific steps of the dyeing are as follows: the slices are rinsed for 3 to 5 times by using distilled water, soaked in 1 percent by weight of iron vitriol solution for 10 minutes, rinsed for 3 to 5 times by using distilled water, and finally dyed in 1 percent by weight of red dye solution for 0.5 to 1d.
Further, the specific steps of dehydration are as follows: rinsing the dyed slice to remove dye liquor, sequentially placing the slice in 30% v/v ethanol solution for soaking for 5min,50% v/v ethanol solution for 5min,70% v/v ethanol solution for 5min,80% v/v ethanol solution for 5min,0.5% solid green dye liquor for 10-40 s,90% v/v ethanol solution for 5min, absolute ethanol for 3min, and storing in absolute ethanol for standby.
Further, the transparent specific steps are as follows: sequentially soaking the dehydrated slices in clove oil for 15min, soaking in xylene for 5min, and storing in xylene for later use.
Further, the specific steps of the sealing sheet are as follows: placing clean glass slide on paper support at fixed position, placing slice on glass slide with forceps, dripping neutral gum, covering with cover glass, sealing, drying in shade or oven drying, and observing.
Compared with the prior art, the invention has the beneficial effects that:
(1) The method of the invention combines the soaking and boiling with the glycerol-absolute ethyl alcohol preservation, effectively improves the softening effect, obviously shortens the boiling time, is easy to slice, has better slicing effect, clear slicing texture, is very beneficial to observing the microstructure of the rosewood, is beneficial to observing and identifying the rosewood, improves the observation and identification efficiency, and realizes more accurate and faster rosewood identification.
(2) The method has the advantages of convenient operation, time and labor saving, low manufacturing cost and capability of better meeting the manufacturing requirements of the cut rosewood.
Drawings
FIG. 1 is a photograph showing the microstructure of a cross-section of example 1 of the present invention;
FIG. 2 is a photograph of a microstructure of a diametral tangential cut of example 1 of the present invention;
FIG. 3 is a photograph of a chord cut microstructure according to example 1 of the present invention;
FIG. 4 is a photograph of a conventional method cross-section microstructure;
FIG. 5 is a photograph of a conventional method of cutting a microstructure of a section;
FIG. 6 is a photograph of a conventional chordal section microstructure.
Detailed Description
In order to better understand the technical content of the present invention, the following provides specific examples to further illustrate the present invention.
The experimental methods used in the embodiment of the invention are conventional methods unless otherwise specified.
Materials, reagents, and the like used in the examples of the present invention are commercially available unless otherwise specified.
The microtome of the present invention uses a flat-pushing microtome lycra SM2010R or a paraffin microtome Germany SLEE-CUT 4062
Example 1-slicing method suitable for use in rosewood identification
Comprises the processes of softening, slicing and tabletting, and the specific processes are as follows:
(1) Softening: sampling the pear heartwood with the age of 30 years, wherein the specification of a sample is 2cm x 2cm; soaking the sample in clear water overnight (soaking for 12 h), boiling in water for 1h, cooling, sucking the water stain on the surface with filter paper, and storing in glycerol-absolute ethanol (v/v=1:2) for more than 1h for later use.
(2) Slicing: slicing was performed using a flat push microtome.
(3) And (3) tabletting: comprises the steps of dyeing, dehydrating, transparentizing and sealing.
S1 dyeing: the sections were rinsed 4 times with distilled water, then soaked in 1% wt. iron vitriol for 10min, rinsed 4 times with distilled water, and finally dyed in 1% wt. red dye for 1d.
S2, dehydration: and rinsing the dyed slice to remove the dye liquor, sequentially placing the slice in 30% v/v ethanol solution for soaking for 5min,50% v/v ethanol solution for 5min,70% v/v ethanol solution for 5min,80% v/v ethanol solution for 5min,0.5% solid green dye liquor for 25s,90% v/v ethanol solution for 5min, and absolute ethanol for 3min, and storing in absolute ethanol for standby.
S3 is transparent: sequentially soaking the dehydrated slices in clove oil for 15min, soaking in xylene for 5min, and storing in xylene for later use.
S4, sealing piece: placing clean glass slide on paper support at fixed position, placing slice on glass slide with forceps, dripping neutral gum, covering with cover glass, sealing, drying in shade or oven drying, and observing.
Example 2-preparation method of cut pieces suitable for use in rosewood identification
Comprises the processes of softening, slicing and tabletting, and the specific processes are as follows:
(1) Softening: sampling the pear heartwood with the age of 30 years, wherein the specification of a sample is 2cm x 2cm; soaking the sample in clear water overnight (soaking for 12 h), boiling in water for 0.9h, cooling, sucking the water stain on the surface with filter paper, and storing in glycerol-absolute ethanol (v/v=1:2) for more than 1h for later use.
(2) Slicing: slicing was performed using a flat push microtome.
(3) And (3) tabletting: comprises the steps of dyeing, dehydrating, transparentizing and sealing.
S1 dyeing: the sections were rinsed 3 times with distilled water, then soaked in 1% wt. iron vitriol for 10min, rinsed 5 times with distilled water, and finally dyed in 1% wt. red dye for 0.5d.
S2, dehydration: and rinsing the dyed slice to remove dye liquor, sequentially placing the slice in 30% v/v ethanol solution for soaking for 5min,50% v/v ethanol solution for 5min,70% v/v ethanol solution for 5min,80% v/v ethanol solution for 5min,0.5% solid green dye liquor for 40s,90% v/v ethanol solution for 5min, and absolute ethanol for 3min, and storing in absolute ethanol for standby.
S3 is transparent: sequentially soaking the dehydrated slices in clove oil for 15min, soaking in xylene for 5min, and storing in xylene for later use.
S4, sealing piece: placing clean glass slide on paper support at fixed position, placing slice on glass slide with forceps, dripping neutral gum, covering with cover glass, sealing, drying in shade or oven drying, and observing.
Example 3-preparation method of cut pieces suitable for use in rosewood identification
Comprises the processes of softening, slicing and tabletting, and the specific processes are as follows:
(1) Softening: sampling the pear heartwood with the age of 30 years, wherein the specification of a sample is 2cm x 2cm; soaking the sample in clear water overnight (soaking for 12 h), boiling in water for 0.8h, cooling, sucking the water stain on the surface with filter paper, and storing in glycerol-absolute ethanol (v/v=1:2) for more than 1h for later use.
(2) Slicing: slicing was performed using a flat push microtome.
(3) And (3) tabletting: comprises the steps of dyeing, dehydrating, transparentizing and sealing.
S1 dyeing: the sections were rinsed 5 times with distilled water, then soaked in 1% wt. iron vitriol for 10min, rinsed 3 times with distilled water, and finally dyed in 1% wt. red dye for 2d.
S2, dehydration: and rinsing the dyed slice to remove dye liquor, sequentially placing the slice in 30% v/v ethanol solution for soaking for 5min,50% v/v ethanol solution for 5min,70% v/v ethanol solution for 5min,80% v/v ethanol solution for 5min,0.5% solid green dye liquor for 10s,90% v/v ethanol solution for 5min, and absolute ethanol for 3min, and storing in absolute ethanol for standby.
S3 is transparent: sequentially soaking the dehydrated slices in clove oil for 15min, soaking in xylene for 5min, and storing in xylene for later use.
S4, sealing piece: placing clean glass slide on paper support at fixed position, placing slice on glass slide with forceps, dripping neutral gum, covering with cover glass, sealing, drying in shade or oven drying, and observing.
Slices of different sections were made from 30 years old flower pear heartwood (Hainan Haikou) according to examples 1-3, and photographs were taken, including cross section, diameter section, chord section. And photographing the prepared cross section slice, the prepared chord section slice and the prepared diameter section slice under a digital biological microscope respectively to obtain a cross section microstructure diagram, a diameter section microstructure diagram and a chord section microstructure diagram. And compared with slices prepared by the conventional method. The results are shown in Table 1 below.
TABLE 1
| Example 1 | Example 2 | Example 3 | Conventional method | |
| Photographic image of microstructure of cross section | Clear texture | Clear texture | Clear texture | Unclear texture |
| Microscopic structure of diameter section | Clear texture | Clear texture | Clear texture | Unclear texture |
| Microscopic structure of tangential plane | Clear texture | Clear texture | Clear texture | Unclear texture |
The results in the table 1 show that the slices prepared by the method in the examples 1-3 are clear in texture, easy to observe and beneficial to the observation and identification of rosewood, and the boiling time is obviously shortened, so that the convenience of observation and identification is improved.
The control example adopts the traditional long-time boiling method, and the slices prepared by the method have unclear textures, are unfavorable for the observation and identification of rosewood, have long boiling time and consume more heart of workers.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (9)
1. The slicing manufacturing method suitable for identifying the rosewood is characterized by comprising the steps of softening, slicing and tabletting, wherein the specific steps of softening are as follows: soaking the sample in clear water overnight, boiling in water for 0.8-1 h, cooling, sucking the surface water stain with filter paper, and storing in glycerol-absolute ethanol for later use.
2. The method for preparing the slices suitable for identifying rosewood according to claim 1, wherein the rosewood sample is sampled over 30 years of heartwood.
3. The method for preparing the slices suitable for identifying rosewood according to claim 1, wherein the volume ratio of glycerin to absolute ethyl alcohol in the glycerin-absolute ethyl alcohol is 1:2.
4. the method for producing the slices suitable for identifying the rosewood according to claim 1, wherein the slicing machine is a flat-pushing slicing machine or a paraffin slicing machine.
5. The method for preparing the slices suitable for identifying rosewood according to claim 1, wherein the step of preparing the slices comprises the steps of dyeing, dehydrating, transparentizing and sealing.
6. The method for making the slices suitable for identifying rosewood according to claim 1, wherein the specific steps of dyeing are as follows: the slices are rinsed for 3 to 5 times by using distilled water, soaked in 1 percent by weight of iron vitriol solution for 10 minutes, rinsed for 3 to 5 times by using distilled water, and finally dyed in 1 percent by weight of red dye solution for 0.5 to 1d.
7. The method for making the slices suitable for identifying rosewood according to claim 1, wherein the specific steps of dehydration are as follows: rinsing the dyed slice to remove dye liquor, sequentially placing the slice in 30% v/v ethanol solution for soaking for 5min,50% v/v ethanol solution for 5min,70% v/v ethanol solution for 5min,80% v/v ethanol solution for 5min,0.5% solid green dye liquor for 10-40 s,90% v/v ethanol solution for 5min, absolute ethanol for 3min, and storing in absolute ethanol for standby.
8. The method for making the slices suitable for identifying rosewood according to claim 1, wherein the specific transparent steps are as follows: sequentially soaking the dehydrated slices in clove oil for 15min, soaking in xylene for 5min, and storing in xylene for later use.
9. The method for making the slices suitable for identifying the rosewood according to claim 1, wherein the specific steps of the sealing slice are as follows: placing clean glass slide on paper support at fixed position, placing slice on glass slide with forceps, dripping neutral gum, covering with cover glass, sealing, drying in shade or oven drying, and observing.
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| CN202310493231.XA CN116558919A (en) | 2023-05-04 | 2023-05-04 | Slicing manufacturing method suitable for identifying rosewood |
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