CN116555078A - Bacillus subtilis for effectively degrading cypermethrin and application thereof - Google Patents

Bacillus subtilis for effectively degrading cypermethrin and application thereof Download PDF

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Publication number
CN116555078A
CN116555078A CN202310264488.8A CN202310264488A CN116555078A CN 116555078 A CN116555078 A CN 116555078A CN 202310264488 A CN202310264488 A CN 202310264488A CN 116555078 A CN116555078 A CN 116555078A
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bacillus subtilis
cypermethrin
strain
pyrethroid
food
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刘书亮
张梦梅
杨坤
杨励
谷欣悦
李建龙
胡凯弟
李琴
邹立扣
刘爱平
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Sichuan Agricultural University
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Sichuan Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/22—Organic substances containing halogen
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention relates to the field of reducing pollutants in the environment or food by utilizing microorganisms, in particular to bacillus subtilis which is separated from healthy broiler manure and has higher degradation capability on cypermethrin. Bacillus subtilis capable of effectively degrading cypermethrinBacillus subtilis) The strain number is J6, and the Chinese microorganism strain preservation managementThe committee general microbiological center registers for preservation; the preservation number is CGMCC No. 26129, and the preservation date is 2022, 12 and 27. The strain can degrade cypermethrin and has good degradation effect on other pyrethroid pesticides and the main degradation product, namely 3-phenoxybenzoic acid. The bacillus subtilis J6 can develop corresponding biological agents for reducing pyrethroid in environment, feed and food; or the strain is developed into health-care food or medicine, so that the harm of pyrethroid pesticides to human body exposure is reduced.

Description

Bacillus subtilis for effectively degrading cypermethrin and application thereof
Technical Field
The invention relates to the field of reducing pollutants in environment or feed food by utilizing microorganisms, in particular to bacillus subtilis which is separated and screened from the cecum content of healthy broilers and has good degradation capability on cypermethrin.
Background
The pyrethroid pesticide has stable light and heat, wide pesticidal spectrum, high pesticidal activity, and high stomach toxicity and contact killing effect on farm and forest pests, such as lepidoptera, on cereal grain crops, melons, fruits and vegetables, and sanitary pests, such as mosquitoes, flies, cockroaches, and the like, so that the pyrethroid pesticide is widely applied to pesticide compounding and pesticide aerosol.
Pyrethroid pesticides have been considered as low-toxicity and safe-to-use pesticides in the past, but as the use amount of pyrethroids is increased, various problems such as environmental pollution, food safety, adverse effects on human health and the like caused by the pyrethroid are increasingly prominent, and the pyrethroid pesticides are attracting attention in various countries around the world. Because of the good stability and durability of pyrethroid pesticides in soil and water, background residues in the environment are continuously accumulated due to mass use, so that the quality of agricultural products and the food safety are directly affected, and the health of human beings is threatened.
Most of the pyrethroid degrading bacteria obtained at present are separated from the environment such as soil, sludge and the like polluted by pesticides, cannot be applied to removing pyrethroid pesticides in foods, and are difficult to be added into feeds and foods to relieve chronic damages of the pesticides to animals and human bodies. Pesticide residue is a long-standing and unavoidable potential safety hazard, but no effective removal method and effective removal way can be used for reducing pesticide residue in the food field, the livestock field and the human body, so that the development of probiotic strain resources with pesticide reducing capability is necessary.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide a bacillus subtilis capable of effectively degrading cypermethrinBacillus subtilis) J6 and uses thereof.
The strain provided by the invention is bacillus subtilisBacillus subtilis) The strain No. J6 is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center), the preservation place is North Xielu No. 1 institute of the Korean area of Beijing, the preservation number is CGMCC No. 26129, and the preservation date is 2022, 12 months and 27 days.
The invention provides separation, screening and identification of animal-derived pesticide degrading bacterial strains.
Taking 0.1-0.5 g of the cecum content of a healthy animal, adding 2 mL modified basal medium (the concentration of cypermethrin is 10-50 mg/L), fully swirling, transferring to a penicillin bottle, and placing at 37 ℃ and culturing at 160 rpm for 2-5 d in an oscillating way. After the cultivation is finished, adding 2 mL acetonitrile, carrying out ultrasonic extraction for 30 min, uniformly mixing, transferring 1.5 mL into an EP tube, centrifuging at 12000 rpm for 10 min, taking supernatant, passing through a 0.22 mu m organic filter membrane, collecting filtrate, measuring the concentration of cypermethrin by using High Performance Liquid Chromatography (HPLC), and calculating the degradation rate of the cecum content on the cypermethrin. The culture medium without cecum content was used as a control.
Weighing 0.1-1 g of the obtained cecum content sample with the cypermethrin degradation effect, adding 9-9.9 mL of physiological saline, carrying out gradient dilution after vortex mixing uniformly, and coating the diluted solution on a solid culture medium of an improved basic culture medium for 2-4 d by aerobic or anaerobic culture at 37 ℃. Selecting strains with different colony morphologies, carrying out repeated streak purification until the colony morphologies on the same plate are uniform, and carrying out inclined plane preservation. Inoculating the slant strain into 5mL of LB liquid medium in a loop, culturing for 2-4 d at 37 ℃, inoculating into 30 mL of LB liquid medium according to 2% of inoculation amount, and culturing for 1-5 d at 37 ℃ and 160 rpm in a shaking way.
Adding 4mL of acetonitrile into 1mL of culture medium, ultrasonically extracting for 30 min, uniformly mixing the extracting solution, centrifuging 1.5 mL liquid (12000 rpm,10 min), taking supernatant, passing through a 0.22 mu m organic filter membrane, collecting filtrate, measuring the concentration of cypermethrin by using High Performance Liquid Chromatography (HPLC), and calculating the degradation rate of the strain on the cypermethrin.
The HPLC detection method comprises the following steps: gemini 100A C18 chromatographic column (4.6 mm ×150 mm,5 μm), acetonitrile: ultrapure water=83:17 as mobile phase, flow rate of 1.0 mL/min, column temperature of 25deg.C, sample injection amount of 10 μl, detector of ultraviolet detector, and detection wavelength of 210 nm.
The formula of the degradation rate of cypermethrin is as follows:
(C 0 -C)/C 0 *100%
wherein:C 0 concentration of cypermethrin (mg/L) in control;Cconcentration of cypermethrin residue after addition of cecal content/strain (mg/L).
Identification of strain morphology: the plate colony is round or elliptic, and has a slight grey-white yellow color, a central bulge, surface wrinkles and moist interior. Gram staining was positive and rod-like.
And (3) strain physiological and biochemical identification: the strain can ferment D-glucose, D-fructose, D-mannose, D-cellobiose, sucrose, trehalose, xylose and gentiobiose, hydrolyze starch, assimilate D-mannitol and N-acetylglucosamine, grow 7% NaCl, grow pH 5, and have positive V-P test, positive catalase, citrate and hydrolyzed gelatin, and not propionate.
And (3) identifying the strain J6 by molecular genetics.
Strain J6 was sequenced by 16S rDNA to obtain the following sequence:
CCCTTGGCCGTATTCTAATAATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTCTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAGGTGAACCGGG。
the sequence of the 16S rDNA gene of the strain is subjected to Blast homology comparison with sequences in GenBank database, and the sequence of the strain is compared with that of the strainBacillus subtilisThe gene sequence is highly homologous, the accession number is OQ195114, and the strain J6 is finally identified as bacillus subtilis by combining morphological and physiological biochemical indexes.
The strain capable of efficiently adsorbing the cypermethrin is obtained by separating and screening the content of the cecum of healthy broilers, has the same degradation capability on the main degradation products of the cypermethrin, the deltamethrin, the fenvalerate and the pyrethroid pesticides, namely the 3-phenoxybenzoic acid, and can be applied to the reduction of residual cypermethrin in the environment and foods.
The culture medium used in the invention is as follows: improving a basal medium: inulin 4g, glucose 4g, beef extract 2g, peptone 2g, yeast powder 2g, naCl 2 2 g，K 2 HPO 4 0.04 g，KH 2 PO 4 0.04 g，MgSO 4 ·4H 2 O 0.01 g，CaCl 2 0.01 g，NaHCO 3 2g, L-cysteine 0.5. 0.5 g, tween-80.2 g, bile salt 0.5. 0.5 g, distilled water 1000 mL, and sterilizing at 121deg.C for 15min. LB medium: tryptone 10 g, yeast extract 5g, naCl 10 g, tween-80 2g, distilled water to 1000 mL, and sterilizing at 121deg.C for 20 min. Adding 2% agar to obtain corresponding solid culture medium.
Compared with the prior art, the invention has the following positive effects: the bacillus subtilis with the capability of degrading pyrethroid pesticides such as cypermethrin and intermediate products 3-phenoxybenzoic acid is obtained from the cecum content of healthy animals for the first time, and the bacillus subtilis is safe in source and can provide a good bacterial source for reducing and removing residual cypermethrin in the environment and food and in animals and human bodies.
Drawings
FIG. 1 shows the effect of culture temperature on the degradation of cypermethrin by the strain.
FIG. 2 shows the effect of substrate concentration on the degradation of cypermethrin by the strain.
FIG. 3 shows the effect of metal ions on the degradation of cypermethrin by the strain.
Description of the embodiments
Example 1: degradation characteristics of B.subtilis J6 on cypermethrin.
1) The effect of the culture temperature on the degradation of the cypermethrin of the J6 strains is that the seed solution of the J6 strains is 2 percentv/v) The inoculum size was inoculated into LB medium with a cypermethrin concentration of 50 mg/L, and cultured at 20, 25, 30, 37 and 45℃with shaking at 160 rpm, respectively. Using cypermethrin working solution without thallus at different temperatures as positive control, using [0010]]And [0011]The method determines the degradation rate of J6 to cypermethrin.
2) The influence of the substrate concentration on the degradation of the cypermethrin by the J6 strain J6 seed solution is calculated to be 2 percentv/v) The inoculation amount is respectively inoculated to the compositions with the concentration of 20, 50, 80, 100 and 150 In LB medium of mg/L cypermethrin, shake culture was carried out at 37℃and 160 rpm for 3d. By [0010]And [0011]The method determines the degradation rate of J6 to cypermethrin.
3) The influence of metal ions on J6 degradation of cypermethrin makes the strain J6 seed liquid be 2%v/v) Inoculating to the seed containing 50 mg/L cypermethrin and 0.01% concentrationw/v) Is different from the metal salt (CuSO) 4 、MnSO 4 、ZnSO 4 、CdCl 2 、Pb (CH 3 COO) 2 ) In LB medium of (C), shaking culture was carried out at 37℃and 160 rpm for 3d. By [0010]And [0011]The method determines the degradation rate of J6 to cypermethrin.
The degradation characteristics of strain J6 on cypermethrin are shown as follows: the degradation of the strain J6 to the cypermethrin is not greatly influenced by the temperature, and the degradation rate of the strain J6 is over 40 percent within the range of 20-45 ℃, and the degradation rate is at 37 ℃ at the highest; in the concentration range of 20-150 mg/L, the degradation rate is reduced along with the increase of the substrate concentration; cd (cadmium sulfide) 2+ 、Zn 2+ And Cu 2+ Has different degrees of inhibition effects on the degradation of cypermethrin by the strain J6, mn 2+ And Pb 2+ Has no influence on degradation effect. The results of this study provide a data reference for the degradation of residual cypermethrin in environmental and food applications of strain J6.
Example 2: the degradation effect of the bacillus subtilis J6 on other pyrethroid pesticides.
The seed solution of the strain J6 is inoculated into LB liquid medium containing 50 mg/L deltamethrin and fenvalerate according to the inoculum size of 2 percent, and is cultured for 3 days at 37 ℃ in a shaking way, and the degradation rate of deltamethrin and fenvalerate is measured by the method described in [0010] and [0011 ].
The strain J6 has better degradation capacity on deltamethrin and fenvalerate, and the degradation rates are 65.48% and 14.68% respectively.
Example 3: the degradation effect of the bacillus subtilis J6 on the main intermediate product 3-phenoxybenzoic acid of the pyrethroid pesticide.
The strain is inoculated into LB liquid medium containing 50 mg/L3-phenoxybenzoic acid according to the inoculum size of 2 percent, shaking culture is carried out at 37 ℃ for 24 h, 1mL of culture solution is respectively taken at 0 h and 24 h, 4mL of acetonitrile is added, ultrasonic extraction is carried out for 30 min, 1.5 mL is removed after uniform mixing, supernatant is taken after centrifugation for 10 min through 12000 r/min, an organic filter membrane of 0.22 mu m is adopted, and the degradation rate of the filtrate on the 3-phenoxybenzoic acid is collected and measured. Chromatographic conditions: gemini 100A C18 chromatographic column (4.6 mm ×150 mm,5 μm), mobile phase acetonitrile, pH 2.5 phosphoric acid water=55:45, flow rate 0.7 mL/min, column temperature 25 ℃, sample injection amount 10 μl, detector ultraviolet detector, and detection wavelength 210 nm.
The results showed that the degradation rate of strain J6 to 50 mg/L3-phenoxybenzoic acid was 76.12%.
Example 4: the bacillus subtilis J6 has the degradation effect on cypermethrin in cow milk.
Preparing cow milk: dissolving 20 g skimmed milk powder in 1000 mL distilled water, and sterilizing at 115deg.C for 15min. A volume of cypermethrin was added to give a final cypermethrin concentration of 50 mg/L.
2 percent of seed solution of the strain J6v/v) The inoculum size was inoculated into cow milk having a cypermethrin concentration of 50 mg/L, respectively, and cultured at 37℃under shaking at 160 rpm. Samples were taken at 0 h, 1d, 2d and 3d, respectively, with [0010]]And [0011]The method is used for measuring the degradation rate of cypermethrin.
The degradation rates of the strain J6 on cypermethrin in 1d, 2d and 3d are 26.73%, 36.41% and 52.56%, respectively, which shows that the strain J6 has good degradation effect on cypermethrin in cow milk.
The above examples are intended to illustrate embodiments of the invention and not to limit the scope of the invention, and modifications or variations may be made to the above description by those skilled in the art, all of which fall within the scope of the invention as defined in the appended claims. Aiming at the problems existing in the prior art, the invention aims to provide a bacillus subtilis capable of effectively degrading cypermethrinBacillus subtilis) J6 and uses thereof.

Claims (6)

1. Bacillus subtilis strainBacillus subtilis) J6, which is characterized by being preserved in China general microbiological culture collection center (CGMCC No. 1, the preservation place is North Chen West Lu No. 1, the Korean area of Beijing city).2629, storage date 2022, 12, 27.
2. The bacillus subtilis according to claim 1Bacillus subtilis) J6, wherein: the strain is separated from healthy cecum of broiler chickens, and has circular or elliptic colony morphology, light yellow gray color, central bulge, surface wrinkles, moist interior, gram positive and rod-shaped cells.
3. The bacillus subtilis according to claim 1Bacillus subtilis) J6, wherein: the degradation capability of the cypermethrin is little affected by temperature within 20-45 ℃ and is not affected by manganese ions and lead ions.
4. The bacillus subtilis according to claim 1Bacillus subtilis) J6, wherein: can degrade various pyrethroid pesticides such as cypermethrin, deltamethrin, fenvalerate and the like, and has good degradation capability on 3-phenoxybenzoic acid which is a main intermediate product for degrading the pyrethroid pesticide.
5. The bacillus subtilis according to claim 1Bacillus subtilis) The application of J6 is characterized in that: can be used for reducing residual cypermethrin in cow milk.
6. The bacillus subtilis according to claim 1Bacillus subtilis) The application of J6 is characterized in that: provides a good bacterial source for removing residual pyrethroid pesticides in the environment or food, prepares a microbial inoculum with the function of reducing the pyrethroid, is applied to the fields of feed, food or environment, and reduces the exposure of human bodies to the pyrethroid pesticides.
CN202310264488.8A 2023-03-20 2023-03-20 Bacillus subtilis for effectively degrading cypermethrin and application thereof Pending CN116555078A (en)

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