CN116550399B - Injection pump for micro-flow control and control method thereof - Google Patents
Injection pump for micro-flow control and control method thereof Download PDFInfo
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- CN116550399B CN116550399B CN202310594675.2A CN202310594675A CN116550399B CN 116550399 B CN116550399 B CN 116550399B CN 202310594675 A CN202310594675 A CN 202310594675A CN 116550399 B CN116550399 B CN 116550399B
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000002347 injection Methods 0.000 title claims abstract description 22
- 239000007924 injection Substances 0.000 title claims abstract description 22
- 238000006073 displacement reaction Methods 0.000 claims abstract description 53
- 238000009530 blood pressure measurement Methods 0.000 claims abstract description 3
- 238000007906 compression Methods 0.000 claims description 20
- 230000006835 compression Effects 0.000 claims description 18
- 238000005086 pumping Methods 0.000 claims description 16
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 238000003786 synthesis reaction Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 description 7
- 239000012530 fluid Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P90/00—Enabling technologies with a potential contribution to greenhouse gas [GHG] emissions mitigation
- Y02P90/02—Total factory control, e.g. smart factories, flexible manufacturing systems [FMS] or integrated manufacturing systems [IMS]
Abstract
The application relates to the technical field of microfluidic control and discloses a microfluidic injection pump and a control method thereof, wherein the microfluidic injection pump comprises an injector, a displacement driving assembly, a displacement sensor, a pressure sensor and an electric on-off valve, and a power output end of the displacement driving assembly is connected to a piston rod of the injector and is used for driving the piston rod to generate displacement; the displacement sensor is connected to the piston rod of the injector and is used for measuring the displacement distance of the piston rod; the outlet of the injector is provided with a pressure sensor for measuring the pressure value at the outlet of the injector; an electric on-off valve is arranged at the outlet of the injector and used for controlling the on-off of the outlet of the injector; the pressure sensor performs pressure measurement according to a displacement threshold value measured by the displacement sensor, and the electric on-off valve performs on-off control according to the pressure threshold value measured by the pressure sensor and the displacement threshold value measured by the displacement sensor. The injection pump has the advantage of being beneficial to improving the air quantity and the air pressure control accuracy of the injection pump.
Description
Technical Field
The application relates to the field of microfluidic control, in particular to a syringe pump for microfluidic control and a control method thereof.
Background
Microfluidic technology is a scientific technology that precisely controls and manipulates microscale fluids to manipulate fluids in a micro-nanoscale space as a major feature.
Aiming at the preparation method of lipid nanoparticle products with micro-liter mixing amount, the current scheme is as follows: the two-phase liquid passes through the microfluidic chip, so that a laminar flow effect is generated on the two-phase liquid, a two-phase interface is formed, two-phase components can controllably react in a diffusion mode on the interface, and the reaction result is controlled to the greatest extent. Referring to fig. 1, in the conventional microfluidic sample injection mode, a two-phase trace sample is firstly sucked into a first sample loading well 61 and a second sample loading well 62 of a chip through a pipette, then a syringe is connected with a sample inlet 64 of a microfluidic chip 60, a piston rod 10 of the syringe is pushed manually or electrically to enable a piston 11 in the syringe to move inwards, compressed gas in the syringe is extruded into a sample loading well sealing cavity 66 of the microfluidic chip 60 by compressing the internal gas volume 20 of the syringe, and the two-phase sample in the sample loading well is pressed into a microfluidic channel 65 by the compressed gas until the whole operation of extruding liquid in the sample loading wells 61 and 62 is finished.
However, the existing solutions generally suffer from the disadvantage that the two-phase liquid in the on-chip wells 61 and 62 is not pushed clean with residual sample, or that the mixed sample in the well 63 is easily splashed out of the microfluidic chip 60 by excessive amounts of gas. The reasons for this defect, deficiency, are: the gas pressure at the outlet of the syringe pump head and the amount of pumped gas are not controlled.
Disclosure of Invention
In order to help to improve the accuracy of controlling the gas pressure and the pumped gas amount at the outlet of the pump head of the injection pump, the application provides an injection pump for micro-flow control and a control method thereof.
On one hand, the injection pump for micro-flow control provided by the application adopts the following technical scheme:
the injection pump for the microfluidic control comprises an injector, a displacement driving assembly, a displacement sensor, a pressure sensor and an electric on-off valve, wherein the power output end of the displacement driving assembly is connected to a piston rod of the injector and used for driving the piston rod to displace; the displacement sensor is connected to a piston rod of the injector and is used for measuring the displacement distance of the piston rod; a pressure sensor is arranged at the outlet of the injector and is used for measuring the pressure value at the outlet of the injector; an electric on-off valve is arranged at the outlet of the injector and used for controlling the on-off of the outlet of the injector; the pressure sensor is used for measuring pressure according to a displacement threshold value measured by the displacement sensor, and the electric on-off valve is used for on-off control according to the pressure threshold value measured by the pressure sensor and the displacement threshold value measured by the displacement sensor.
By adopting the technical scheme, in the use process, the electric on-off valve is closed, the displacement setting threshold of the piston in the injector can be realized by combining the displacement driving component with the displacement sensor, the gas compression is realized, and the pressure of the gas is increased in the compression process of the piston; the pressure sensor is arranged at the outlet of the injector, so that the pressure value at the outlet of the injector can be measured and controlled, and the control of the gas pressure at the outlet of the pump head of the injection pump is facilitated; after the gas is boosted, the electric on-off valve is opened to realize the pumping of the gas, and the pressure sensor can measure the pressure decay value of the pumped gas, so that the electric on-off valve can be timely turned off, thereby being beneficial to controlling the gas quantity pumped out, and being beneficial to reducing the situations that the liquid quantity in the sample well on the chip is not pushed cleanly, the residual sample exists, and the excessive gas quantity easily splashes out the mixed sample in the sample well.
Optionally, the syringe exit includes main road and branch road, electronic on-off valve with pressure sensor is located on the main road, install the ooff valve on the branch road, the branch road with the link on the main road is located electronic on-off valve is close to one side of the last piston rod of syringe.
Through adopting above-mentioned technical scheme, set up branch road and ooff valve, after the gas pump is gone into, electronic on-off valve is closed, opens the ooff valve and is favorable to the reduction of syringe, makes things convenient for the operation next time.
Optionally, the switch valve is set as a one-way valve, and the one-way flow direction of the one-way valve is from the branch to the main path.
By adopting the technical scheme, the switch valve adopts the one-way valve, so that substances can only enter and not exit, the sealing state is maintained in the compression process, and independent signal control is not needed.
Optionally, the displacement driving assembly comprises an electric push rod, and the electric push rod is connected to the piston rod through a connecting piece.
By adopting the technical scheme, the electric push rod has better control and accuracy, and is favorable for ensuring the accuracy of displacement.
On the other hand, the injection pump control method for the micro-flow control adopts the following technical scheme:
the control method of the injection pump for the micro-flow control based on the injection pump for the micro-flow control comprises the following steps:
acquiring compression stroke L AB And a feeding stroke L BC Compression stroke L AB Corresponding to a path between the origin position A and the first setting position B; feeding stroke L BC Corresponding to a path between the first setting position B and the second setting position C; compression stroke L AB And a feeding stroke L BC According to the volume V of the syringe Total (S) Injector travel L Total (S) Sample composition E and pump head pressure P 2 Calculating to obtain;
the displacement sensor is used for measuring the position information of the injector piston;
opening an electric on-off valve, and returning the piston to zero;
when the piston returns to the zero position A, the electric on-off valve is closed;
when the piston moves by the compression stroke L AB After that, the pressure sensor measures a first pressure value P A ；
When the piston moves to feed the material L BC After that, the pressure sensor measures a second pressure value P B ；
Opening an electric on-off valve, pumping gas, and measuring a real-time third pressure value P by a pressure sensor C ；
When the third pressure value P C Greater than the first pressure value P A When the electric on-off valve is kept open;
when the third pressure value P C Less than or equal to the first pressure value P A And closing the electric on-off valve when the valve is opened.
By adopting the technical scheme, the volume V of the injector is calculated by Total (S) Injector travel L Total (S) Sample composition E and pump head pressure P 2 The system controls the size of the piston moving stroke amount to control the size of the outlet pressure of the injector, and when the piston moves to a specified position to stop, the system reads the pressure decay value of the outlet of the pump head to actually control the amount of pumped gas, so that the control of the pressure of the pump and the amount of pumped gas is realized, and the problems that the liquid amount in the sample well on the chip is not pushed completely, residual samples exist and excessive gas amount easily splashes out mixed samples in the sample well can be effectively avoided.
Optionally, the method further comprises a reset step: and the electric on-off valve is kept to be closed, the piston moves from the second set position C to the original position A, and the on-off valve on the branch is opened.
By adopting the technical scheme, the injector is reset and is convenient for the next operation.
Optionally, the parameter relation in the parameter calculation is:
P 1 .V 1 /T 1 ＝P 2 .V 2 /T 2 ；
wherein, standard atmospheric pressure: p (P) 1 Gas temperature: t (T) 2 And T 1 Syringe volume: v (V) Total (S) Injector stroke: l (L) Total (S) Sample synthesis amount: e, pump head pressure: p (P) 2 。
Optionally, before the piston returns to the set zero position a, the outlet of the injector and the microfluidic chip are in an unconnected state; when the piston returns to the set zero position A, the outlet of the injector and the microfluidic chip are in a connection state before pumping gas.
By adopting the technical scheme, when the pressure is returned to zero, the electric on-off valve is opened, so that the inside of the injector is communicated with the outside, the air with standard atmospheric pressure is sucked into the injector, and the control accuracy of the air quantity and the air pressure is improved; in order to make the piston return to zero without affecting the sample in the chip, the injector is connected with the chip before the piston is compressed, so that the accuracy of the air quantity and air pressure control of the injection pump is improved.
In summary, the present application includes at least one of the following beneficial technical effects: firstly enabling pumped gas to reach a certain pressure through a compression stage, then continuing to give a proper pressure for lifting, then opening an electric stop valve, measuring the decay value of the pressure, and ending pumping operation until the pressure is lower than a pressure threshold value; therefore, the device can pump the gas into the micro-fluidic chip by combining with the control method, thereby being beneficial to controlling the accuracy of the amount of the pumped gas, and controlling the pressure of the pump so as to reduce the situation of sample splashing caused by excessive gas, and ensuring that the sample is not easy to remain in the sample loading well.
Drawings
Fig. 1 is a block diagram of a syringe and a microfluidic chip in the related art, in which a piston rod is pushed manually or electrically.
Fig. 2 is a schematic structural diagram of a syringe pump for microfluidic according to an embodiment of the present application.
Fig. 3 is a top view of the present microfluidic chip.
Fig. 4 is a control flow chart of a method for controlling a syringe pump for microfluidic control according to the present application.
Reference numerals: 10. a piston rod; 11. a piston; 20. a syringe body; 30. a pressure sensor; 40. a one-way valve; 50. an electric shut-off valve; 60. a microfluidic chip; 61. a first sample loading well; 62. a second sample loading well; 63. sampling well; 64. a sample inlet; 65. a microfluidic channel; 66. loading a sample well sealing cavity; 67. discharging a sealed cavity of the sample well; 70. an electric push rod; 71. a push rod; 72. a connecting piece; 80. a displacement sensor.
Detailed Description
The present application is described in further detail below with reference to the accompanying drawings.
The embodiment of the application discloses a syringe pump for microfluidic control.
Referring to fig. 2 and 3, a syringe pump for micro-fluid control includes a syringe, a pressure sensor 30, an on-off valve, an electric on-off valve, a displacement driving assembly, and a displacement sensor 80. In this embodiment, the on-off valve is a check valve 40. The syringe comprises a syringe body 20, a piston 11, a piston rod 10. The electric on-off valve is an electric stop valve 50. The displacement drive assembly employs an electric push rod 70. The pressure sensor 30, the electric shut-off valve 50, the electric push rod 70, and the displacement sensor 80 are all electrically connected to the control system.
The injector body 20 is mounted directly above the microfluidic chip 60 and fixed, and the outlet of the injector is used for communicating with the sample inlet 64 of the microfluidic chip 60. The piston 11 is slidingly coupled inside the syringe body 20, and the piston rod 10 is used to drive the displacement of the piston 11. The electric push rod 70 is mounted right above the syringe and fixed, the piston rod 10 is fixedly connected with the push rod 71 of the electric push rod 70 through a connecting piece 72, and the displacement of the piston rod 10 is driven by the electric push rod 70. The outlet of the injector comprises a main path and a branch path, and a one-way valve 40 is arranged on the branch path; an electric stop valve 50 is installed on the main road. A pressure sensor 30 is installed at the junction of the main and branch lines for monitoring the pressure value of the outlet of the syringe, and the pressure sensor 30 is located at the side of the electric shut-off valve 50 near the piston 11. A displacement sensor 80 is mounted at the junction of the push rod 71 and the piston rod 10 for measuring the position of the syringe pump piston 11. The electric push rod 70 has better displacement control accuracy.
The pressure sensor 30 performs pressure measurement according to a displacement threshold value measured by the displacement sensor 80, and the electric shut-off valve 50 performs on-off control according to the pressure threshold value measured by the pressure sensor 30 in combination with the displacement threshold value measured by the displacement sensor 80. The method has the advantages that the gas compression distance and the set pressure value range are set firstly, and then the gas is pumped out, so that the improvement of the gas pumping-out amount and the accuracy of pressure control is facilitated.
The injection pump for micro-flow control and the control method thereof in the embodiment of the application are as follows: the movement of the piston 11 within the syringe to the displacement threshold is achieved by the electric push rod 70 in combination with the displacement sensor 80, and the pressure sensor 30 is provided at the outlet of the syringe to detect the pressure state of the gas. In the use process, after the piston 11 compresses the gas with a set distance, the gas has a certain pressure, and the electric stop valve 50 is opened to realize the pumping of the gas; during pumping, the pressure sensor 30 detects decay of pressure; when the value fed back by the pressure sensor 30 is lower than the set threshold value, the electric stop valve 50 is closed, and the gas pumping is finished; therefore, the method is beneficial to improving the accuracy of the gas quantity and pressure control of the gas pump. After the gas is pumped, the electric shutoff valve 50 is closed, and the syringe is reset at this time, so that the outside air can be introduced into the syringe body 20 by the check valve 40, and the operation of the syringe pump is terminated.
The embodiment of the application also discloses a control method of the injection pump for the micro-flow control.
Referring to fig. 3, a microfluidic chip 60 is provided with a first sample loading well 61, a second sample loading well 62, a sample outlet well 63, a sample inlet 64, a microfluidic channel 65, a sample loading well seal cavity 66, and a sample outlet well seal cavity 67. The two-phase sample needs to be moved into the first and second wells 61, 62 of the microfluidic chip 60 prior to the control step.
Referring to fig. 4, the control method includes the steps of:
acquiring compression stroke L AB And a feeding stroke L BC Compression stroke L AB Corresponding to a path between the origin position A and the first setting position B; feeding stroke L BC Corresponding to a path between the first setting position B and the second setting position C; compression stroke L AB And a feeding stroke L BC According to the volume V of the syringe Total (S) Injector travel L Total (S) Sample composition E and pump head pressure P 2 And (5) calculating to obtain the product.
The displacement sensor 80 performs measurement of positional information of the syringe piston 11;
opening the electric shut-off valve 50; judging whether the piston 11 is positioned at a set zero point position A according to the position information of the piston 11 measured by the displacement sensor 80; if not, outputting a return-to-zero signal to the electric push rod 70 to drive the piston 11 to move to the original point position A; when the piston 11 returns to the set zero position a, the electric shut-off valve 50 is closed; before this step, the syringe and the microfluidic chip 60 are in an unconnected state. After the zero return, the injector is connected with the micro-fluidic chip 60 before the gas is pumped in.
When the piston 11 moves by the compression stroke L AB After that, the pressure sensor 30 measures a first pressure value P A ；
When the piston 11 moves to feed the material L BC After that, the pressure sensor 30 measures a second pressure value P B The method comprises the steps of carrying out a first treatment on the surface of the At this time, the inside of the injector is in a sealed state, so that the electric push rod 70 drives the piston 11 to move for a set distance, firstly, quantitative gas is ensured, and secondly, certain pressure of the gas is ensured, thereby being beneficial to pumping the gas.
The electric stop valve 50 is opened, gas is pumped in, and the pressure sensor 30 measures a real-time third pressure value P C I.e., the decay value of the gas within the syringe; and the third pressure value P C And a first pressure value P A Comparing the first pressure value P A Is at one atmosphere.
When the third pressure value P C Greater than the first pressure value P A When the electric shutoff valve 50 is kept open;
when the third pressure value P C Less than or equal to the first pressure value P A At this time, the electric shutoff valve 50 is closed.
During pumping, the electric push rod 70 is stationary, allowing the pressure of the gas to gradually decrease, and when one atmosphere is reached, the pumping is completed. Therefore, the amount of air can be controlled by controlling the on-off time of the electric shut-off valve 50, and overcharge can be avoided.
The injection pump control method for the micro-fluid control provided by the invention can be used for modifying the input syringe volume V Total (S) Injector stroke L Total (S) Sample synthesis amount E and pump head pressure P 2 And the pressure at the outlet of the injection pump and the pumped gas quantity can be quantitatively controlled by the parameters. The specific operation is as follows:
from the ideal gas state equation, P can be known 1 *V 1 /T 1 ＝P 2 *V 2 /T 2 Wherein P is 1 At standard atmospheric pressure, T 2 And T 1 Is the gas temperature. Let us assume that the influence of temperature on its state, i.e. P 1 *V 1 ＝P 2 *V 2 Then the compression stroke of the piston 11 can be expressed as L AB ＝L Total (S) *(P 1 *V 1 )/(V Total (S) *(P 2 +1)), the feeding stroke of the piston 11 can be expressed as L BC ＝L Total (S) *E/V Total (S) . The system will input syringe volume V Total (S) Injector stroke L Total (S) Sample synthesis amount E and pump head pressure P 2 The equal parameters are converted into the movement stroke of the piston 11, the size of the outlet pressure of the injector is controlled by the size of the movement stroke of the piston 11, and the pressure decay value of the outlet of the pump head, namely the third pressure value P, is read when the piston 11 stops moving to a designated position C To actually control the amount of the pumped gas, thereby realizing the control of the pressure of the pump and the amount of the pumped gas.
After the pumping operation is completed, the mixing in the first and second sample wells 61 and 62 is completed. At this time, in order to facilitate the next pumping operation, the control system maintains the closed state of the electric shut-off valve 50, and controls the electric push rod 70 to drive the piston 11 to return to the origin position a. In this process, the check valve 40 is opened so that the outside air enters the syringe body 20, and the outside air is regarded as the standard atmospheric pressure. The use of the check valve 40 prevents the syringe from being reset in the negative pressure environment within the syringe body 20.
In the step of the zeroing operation, it is necessary that the syringe and the microfluidic chip 60 are in a connectionless state, and the electric shut-off valve 50 is opened. After the zero return is finished, the electric stop valve 50 is closed, and the injector and the microfluidic chip 60 can be connected.
The implementation principle of the injection pump control method for the micro-flow control in the embodiment of the application is as follows: first, in the system, an expected target value to be set and reached is input: syringe volume V Total (S) Injector stroke L Total (S) Sample synthesis amount E and pump head pressure P 2 And the parameters are converted into two sections of movement strokes of the piston 11 through calculation, the system controls the size of the outlet pressure of the injector by the movement stroke of the piston 11, and when the piston 11 stops moving to a designated position, the system controls the on-off of the electric stop valve 50 by judging the decay value of the pump outlet pressure to control the pump outlet gas quantity, so that the control of the pump pressure and the pump outlet gas quantity is realized, and the situations that the residual sample is not completely pushed by the liquid quantity in the sample well on the chip and the mixed sample in the sample well 63 is easily splashed out by excessive gas quantity are reduced as much as possible.
The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.
Claims (2)
1. The injection pump for the micro-flow control is characterized by comprising an injector, a displacement driving assembly, a displacement sensor (80), a pressure sensor (30) and an electric on-off valve;
the displacement driving assembly comprises an electric push rod (70), wherein the electric push rod (70) is connected to a piston rod (10) of the syringe through a connecting piece (72) and is used for driving the piston rod (10) to displace;
the displacement sensor (80) is connected to a piston rod (10) of the syringe for measuring a displacement distance of the piston rod (10); a pressure sensor (30) is arranged at the outlet of the injector and is used for measuring the pressure value at the outlet of the injector;
an electric on-off valve is arranged at the outlet of the injector and used for controlling the on-off of the outlet of the injector;
the pressure sensor (30) performs pressure measurement according to a displacement threshold value measured by the displacement sensor (80), and the electric on-off valve performs on-off control according to the pressure threshold value measured by the pressure sensor (30) and the displacement threshold value measured by the displacement sensor (80), wherein the pressure sensor (30) detects decay of pressure in the pumping process; when the value fed back by the pressure sensor (30) is lower than a set threshold value, closing the electric on-off valve;
the outlet of the injector comprises a main path and a branch path, the electric on-off valve is arranged on the main path, the pressure sensor (30) is positioned at the junction of the main path and the branch path, and the junction of the branch path and the main path is positioned at one side of the electric on-off valve, which is close to the upper piston rod (10) of the injector;
the branch is provided with a switch valve, the switch valve is set into a one-way valve (40), and the one-way flow direction of the one-way valve (40) is from the branch to the main;
when the piston (11) of the injector returns to the set zero position A, the electric on-off valve is closed;
when the piston (11) of the injector moves by the compression stroke L AB After that, the pressure sensor (30) measures a first pressure value P A The method comprises the steps of carrying out a first treatment on the surface of the When the piston (11) moves to feed the material L BC After that, the pressure sensor (30) measures a second pressure value P B The method comprises the steps of carrying out a first treatment on the surface of the Opening an electric on-off valve, pumping gas, and measuring a real-time third pressure value P by a pressure sensor (30) C The method comprises the steps of carrying out a first treatment on the surface of the When the third pressure value P C Greater thanFirst pressure value P A When the electric on-off valve is kept open;
when the third pressure value P C Less than or equal to the first pressure value P A Closing the electric on-off valve when the valve is opened;
compression stroke L AB Corresponding to a path between the zero point position A and the first setting position B; feeding stroke L BC Corresponding to a path between the first setting position B and the second setting position C;
before the piston (11) returns to the set zero position A, the outlet of the injector and the microfluidic chip (60) are in an unconnected state;
when the piston (11) returns to the set zero position A, the outlet of the injector is connected with the microfluidic chip (60) before the gas is pumped.
2. A method of controlling a syringe pump for micro-fluidic control according to claim 1, comprising the steps of:
acquiring compression stroke L AB And a feeding stroke L BC The method comprises the steps of carrying out a first treatment on the surface of the Wherein the compression stroke L AB Corresponding to a path between the zero point position A and the first setting position B; the feeding stroke L BC Corresponding to a path between the first setting position B and the second setting position C; the compression stroke L AB And the feeding stroke L BC According to the volume V of the syringe Total (S) Injector travel L Total (S) Sample composition E and pump head pressure P 2 Calculating to obtain;
opening an electric on-off valve;
the displacement sensor (80) is used for measuring the position information of the piston (11) of the injector;
judging whether the piston (11) is positioned at a set zero point position A or not based on the position information of the piston (11) measured by the displacement sensor (80); if not, outputting a return-to-zero signal to the electric push rod (70) so as to drive the piston (11) to move to a zero position A;
when the piston (11) returns to the set zero position A, the electric on-off valve is closed; wherein, before this step, the injector and the microfluidic chip (60) are in an unconnected state;
after the zero return is finished, before the gas is pumped in, connecting the injector and the micro-fluidic chip (60);
when the piston (11) moves by the compression stroke L AB After that, the pressure sensor (30) measures a first pressure value P A ；
When the piston (11) moves to feed the material L BC After that, the pressure sensor (30) measures a second pressure value P B ；
Opening an electric on-off valve, pumping gas, and measuring a real-time third pressure value P by a pressure sensor (30) C ；
Wherein, during the pumping process, the position of the electric push rod (70) is not moved;
when the third pressure value P C Greater than the first pressure value P A When the electric on-off valve is kept open;
when the third pressure value P C Less than or equal to the first pressure value P A Closing the electric on-off valve when the valve is opened;
the electric on-off valve is kept closed, the piston (11) moves from the second set position C to the zero position A, and the on-off valve on the branch is opened;
the parameter relation in the parameter calculation is as follows:
wherein, syringe volume: v (V) Total (S) Injector stroke: l (L) Total (S) Sample synthesis amount: E.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310594675.2A CN116550399B (en) | 2023-05-24 | 2023-05-24 | Injection pump for micro-flow control and control method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310594675.2A CN116550399B (en) | 2023-05-24 | 2023-05-24 | Injection pump for micro-flow control and control method thereof |
Publications (2)
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| CN116550399B true CN116550399B (en) | 2024-03-22 |
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| JPH06347381A (en) * | 1993-06-04 | 1994-12-22 | Aloka Co Ltd | Dispensing apparatus |
| CN1529157A (en) * | 2003-09-27 | 2004-09-15 | 吉林大学 | Microflow controlled chip flow-type biochemical analysis instrument and method for detecting biochemical components |
| CN1603009A (en) * | 2004-10-28 | 2005-04-06 | 北京博奥生物芯片有限责任公司 | Trace amount liquid jet system |
| CN1991370A (en) * | 2005-12-29 | 2007-07-04 | 财团法人工业技术研究院 | Micro-fluid drive and speed control device and method |
| CN102735864A (en) * | 2012-06-28 | 2012-10-17 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Hand-operated air pressure sample introduction method for microfluidic chips |
| CN103245588A (en) * | 2013-05-17 | 2013-08-14 | 国家地质实验测试中心 | Gas content measurement and gas collection device |
| JP2014039893A (en) * | 2012-08-21 | 2014-03-06 | Canon Machinery Inc | Paste-coating apparatus and paste-coating method |
| CN204170206U (en) * | 2014-05-06 | 2015-02-25 | 深圳市尚荣医疗股份有限公司 | Closed high precision micro syringe pump |
-
2023
- 2023-05-24 CN CN202310594675.2A patent/CN116550399B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06347381A (en) * | 1993-06-04 | 1994-12-22 | Aloka Co Ltd | Dispensing apparatus |
| CN1529157A (en) * | 2003-09-27 | 2004-09-15 | 吉林大学 | Microflow controlled chip flow-type biochemical analysis instrument and method for detecting biochemical components |
| CN1603009A (en) * | 2004-10-28 | 2005-04-06 | 北京博奥生物芯片有限责任公司 | Trace amount liquid jet system |
| CN1991370A (en) * | 2005-12-29 | 2007-07-04 | 财团法人工业技术研究院 | Micro-fluid drive and speed control device and method |
| CN102735864A (en) * | 2012-06-28 | 2012-10-17 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Hand-operated air pressure sample introduction method for microfluidic chips |
| JP2014039893A (en) * | 2012-08-21 | 2014-03-06 | Canon Machinery Inc | Paste-coating apparatus and paste-coating method |
| CN103245588A (en) * | 2013-05-17 | 2013-08-14 | 国家地质实验测试中心 | Gas content measurement and gas collection device |
| CN204170206U (en) * | 2014-05-06 | 2015-02-25 | 深圳市尚荣医疗股份有限公司 | Closed high precision micro syringe pump |
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| CN116550399A (en) | 2023-08-08 |
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