CN116539905B

CN116539905B - Sample detection system, sample detection method, and storage medium

Info

Publication number: CN116539905B
Application number: CN202310813361.7A
Authority: CN
Inventors: 罗新强; 刘文杰; 袁云东; 张裕剑
Original assignee: Shenzhen Reetoo Biotechnology Co Ltd
Current assignee: Shenzhen Reetoo Biotechnology Co Ltd
Priority date: 2023-07-05
Filing date: 2023-07-05
Publication date: 2023-09-22
Anticipated expiration: 2043-07-05
Also published as: CN116539905A

Abstract

The application provides a sample detection system, a sample detection method and a storage medium, wherein the sample detection system comprises an elution device, a sample detector and a control device; the control device is used for: responding to an input operation instruction of reagent related information, and acquiring the reagent related information corresponding to the input operation instruction; acquiring sample identity information of a biological sample on an attachment and item association information of a target sample corresponding to the sample identity information; comparing the reagent related information with the project related information, and controlling the elution device to perform elution operation on the target sample so as to form a sample liquid to be detected; the eluting liquid amount corresponding to the eluting operation is a corresponding value of a small value in the reagent related information and the project related information. The sample detection system provided by the application can be matched with different detection samples according to the actual demands of users to select different amounts of suitable reagents, can save the reagents, has high degree of automation, and enhances the user experience.

Description

Sample detection system, sample detection method, and storage medium

Technical Field

The present application relates to the technical field of medical devices, and in particular, but not limited to, a sample detection system, a sample detection method, and a storage medium.

Background

Currently, some samples are sampled from the human body by a sampling tool (such as a flocked swab, etc.), the samples are attached to the sampling tool, and the samples need to be eluted from the sampling tool to form a sample liquid when being detected. Because all need elution to the sample, the consumption of eluent is huge and leads to the cost higher, and the check out test set who sells on the market either does not possess the automatic elution function, or even have the elution function but the eluent volume of joining is fixed and single can not match the differentiation demand of different detection samples, and the eluent volume that leads to for guaranteeing the elution effect is big. In order to solve the technical problem, the application provides a sample detection system and a sample detection method which can be matched with different detection samples to select different eluent amounts and save reagents.

Disclosure of Invention

The application mainly aims to provide a sample detection system, a sample detection method and a storage medium, which can be used for matching different detection samples according to actual demands of users to select different suitable reagent amounts and save reagents.

In a first aspect, the present application provides a sample detection system, comprising an elution device, a sample detector, and a control device, wherein the control device is connected with the elution device and at least one sample detector;

The control device is used for:

responding to an input operation instruction of reagent related information, and acquiring the reagent related information corresponding to the input operation instruction;

acquiring sample identity information of a biological sample on an attachment and item association information of a target sample corresponding to the sample identity information;

comparing the reagent related information with the project related information, and controlling the elution device to perform elution operation on the target sample so as to form a sample liquid to be detected; wherein the amount of the eluent corresponding to the elution operation is a corresponding value of a small value in the reagent-related information and the item-related information;

and controlling the sample detector to perform detection corresponding to the item association information on the sample liquid to be detected.

In a second aspect, the present application provides a sample detection method, applied to the sample detection system, the sample detection method comprising:

Comparing the reagent related information with the project related information, and performing elution operation on the target sample to form a sample liquid to be detected; wherein the amount of the eluent corresponding to the elution operation is a corresponding value of a small value in the reagent-related information and the item-related information;

and executing detection corresponding to the item association information on the sample liquid to be detected.

In a third aspect, the present application provides a storage medium having stored therein an executable program that, when executed, realizes the use of the control device included in the sample detection system described above, or that, when executed, realizes the steps of the sample detection method described above.

The application provides a sample detection system, a sample detection method and a storage medium, wherein the sample detection system comprises an elution device, a sample detector and a control device, and the control device is connected with the elution device and at least one sample detector; the control device is used for: responding to an input operation instruction of reagent related information, and acquiring the reagent related information corresponding to the input operation instruction; acquiring sample identity information of a biological sample on an attachment and item association information of a target sample corresponding to the sample identity information; comparing the reagent related information with the project related information, and controlling the elution device to perform elution operation on the target sample so as to form a sample liquid to be detected; wherein the amount of the eluent corresponding to the elution operation is a corresponding value of a small value in the reagent-related information and the item-related information; and controlling the sample detector to perform detection corresponding to the item association information on the sample liquid to be detected. The sample detection system provided by the application can be matched with different detection samples according to the actual demands of users to select different amounts of suitable reagents, can save the reagents, has high degree of automation, and enhances the user experience.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic block diagram of an alternative sample detection system according to an embodiment of the present application;

FIG. 2 is a schematic block diagram of an alternative sample detection system according to an embodiment of the present application;

FIG. 3 is a schematic diagram of an alternative interface of a sample detection system according to an embodiment of the present application;

FIG. 4 is a schematic diagram of an alternative interface of a sample detection system according to an embodiment of the present application;

FIG. 5A is a schematic perspective view of an alternative sample detection system according to an embodiment of the present application;

FIG. 5B is a partial enlarged view of a portion of a sample detection system according to an embodiment of the present application;

FIG. 5C is a cross-sectional view of a portion of the structure of a sample detection system according to one embodiment of the present application;

FIG. 6 is a schematic diagram of an alternative sample detection system according to an embodiment of the present application;

FIG. 7 is a schematic diagram of another alternative sample detection system according to an embodiment of the present application;

FIG. 8 is a schematic block diagram of an alternative sample detection system according to an embodiment of the present application;

FIG. 9 is a schematic flow chart of an alternative sample detection method according to an embodiment of the present application;

fig. 10 is a schematic flow chart of another alternative sample detection method according to an embodiment of the present application.

Icon: 1000. a sample detection system; 10. a fluid supplementing device; 20. an elution device; 30. first sample information detecting means; 40. a first dilution device; 41. a first dry chemical dilution device; 42. a first microscopic dilution device; 50. a gynecological routine detector; 51. a dry chemistry detector; 52. a microscopic examination detector; 60. a second dilution device; 70. a microecological detector; 71. a pushing device; 72. a film reading device; 73. an aggregation device; 80. a second sample information detection device; 90. a control device; 91. a display unit; 92. an input unit; 93. an output unit; 94. a processor; 95. a memory; 100. an identification device.

Description of the embodiments

The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

In the description of the present application, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.

The application provides a sample detection system 1000, and aims to provide a sample detection system 1000 which can be matched with different detection samples according to the actual demands of users to select different suitable reagent amounts and can save reagents.

Some embodiments of the present application will be described in detail below with reference to the accompanying drawings, and the features of the following examples and embodiments may be combined with each other without conflict.

Referring to fig. 1, fig. 1 is a schematic block diagram illustrating an alternative configuration of a sample detection system 1000 according to an embodiment of the present application. As shown in fig. 1, the sample detection system 1000 includes an elution device 20, a sample detector, and a control device 90, the control device 90 being coupled to the elution device 20 and the at least one sample detector; wherein the control device is used for:

Responding to an input operation instruction of the reagent related information, and acquiring the reagent related information corresponding to the input operation instruction;

comparing the reagent-related information with the item-related information, controlling the eluting device 20 to perform an eluting operation on the target sample to form a sample liquid to be measured; the eluting liquid amount corresponding to the eluting operation is a corresponding value of a small value in the reagent related information and the project related information;

The respective modules shown in fig. 1 and 2 are described below.

As shown in fig. 1 and 2, in some embodiments, the control device 90 includes a display unit 91 and an input unit 92, and the control device 90 externally connects the display unit 91 and the input unit 92 through a communication interface and an I/O interface. The input unit 92 is configured to receive an input operation instruction, and the user may directly input the operation instruction to the control device 90 through the input unit 92, where the input operation instruction includes reagent-related information selected on the display unit 91, or user-defined reagent-related information directly input at an interface on the display unit 91, and the display unit 91 displays the reagent-related information, as shown in fig. 3 and 4. In this way, the control device 90 responds to the input operation instruction of the reagent-related information, and thereby acquires the reagent-related information corresponding to the input operation instruction. The mode of receiving the input operation instruction through the control device 90 can match different detection samples according to the actual demands of the user, so that the direct selection or the indirect selection of different suitable reagent amounts can be finally realized, and the market demands of medical institutions of all layers are met. Wherein the direct selection is direct mapped feedback and transmission, for example: the test tube-related information is a preset amount of the reagent, and the control device 90 acquires the preset amount of the reagent. Indirect selection is to feed back and transmit through other mapping or conversion or scaling, for example: the test tube related information is preset item information, based on which the control device 90 can feed back a preset reagent amount corresponding to the preset item information to the control device 90, and the control device 90 indirectly acquires the preset reagent amount; also for example: the test tube related information is preset item information, a small item value is obtained based on the comparison of the preset item information and the item related information, and the control device 90 performs an elution operation on the target sample according to the small item value to form the liquid to be tested. Further preferably, as shown in fig. 3 and 4, reagent-related information and item-related information may be displayed on the display unit 91 so that the user can intuitively know the enforceability and safety of the elution operation and the fluid replacement operation. The control device 90 may obtain the reagent-related information through the input unit 92, and the input unit 92 includes, but is not limited to, a keyboard, a mouse, a touch screen, or control buttons. In other embodiments, as shown in fig. 3 and 4, the control device 90 further includes an output unit 93, where the output unit 93 is communicatively connected to the display unit 91, and the control device 90 is connected to the output unit 93 through a communication interface and an I/O interface. The output unit 93 is configured to output a detection result of the target sample, amplification processing parameters based on fluid replacement and dilution, and the like, and the display unit 91 is also configured to display a detection result of the target sample, display reagent-related information, item-related information, and the like. If the detection corresponding to the item association information has been performed and the detection result is acquired, the control device 90 controls the output unit 93 to output the detection result of the target sample in response to the output operation instruction. Similar to the input operation instruction, the user may directly output the operation instruction to the control device 90 through the output unit 93, the output operation instruction including various information shown on the display unit or various information custom-output on the display unit 91 for the user to flexibly view, the various information including but not limited to: detecting image results, detecting numerical results, amplifying parameters, reagent amounts and the like. Of course, the control device 90 may also control a printing device (not shown) to print the detection results in a report in combination, or split printing, or the like, according to the output operation instruction.

In some embodiments, the control device 90 further includes a processor 94, a memory 95. The processor 94, memory 95, communication interfaces, and I/O interfaces communicate via a bus. The processor 94 may be a central processing unit (Central Processing Unit, CPU), the processor 94 may also be other general purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuit, ASIC), field-programmable gate arrays (Field-Programmable Gate Array, FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or the like. Wherein the general purpose processor may be a microprocessor or the processor may be any conventional processor or the like. And the memory 95 stores various computer programs such as an operating system and application programs for execution by the processor 94 and data required for execution of the computer programs. In the process of detecting and analyzing the sample to be detected, if necessary, the data stored locally can be stored in the memory 95. For example, the memory 95 of the control device 90 stores, in advance, corresponding preset data information including at least different item-related information and reagent-related information for different items or sub-categories of the same item, for example: target reagent amount, preset reagent amount, etc. Illustratively, the target eluent amount for the gynecological routine item is 800ul, the target eluent amounts for the gynecological routine test item and the microecological item is 1500ul, the target eluent amount for sputum is 1000ul, and so on. And the preset data information also stores information corresponding to dilution operation, for example: the method comprises the steps of a threshold range corresponding to concentration information, a plurality of pieces of different dilution ratio information corresponding to the condition that the characteristic concentration information is too high, detection standards (preset standards) corresponding to each gynecological routine detection item and each microecological detection item, standard forms (preset forms) of smear of wet film microscopy or microecological microscopy, and amplification reference values of detection results. Of course, the memory 95 also stores data such as various detection results.

As shown in the interface diagrams of fig. 3 to 4, in some embodiments, the reagent related information is selected from any one of a preset reagent amount, preset item information, and preset identification information. In other embodiments, the item-related information includes item information to be inspected, or the item-related information includes item information to be inspected and a target amount of reagent corresponding based on the item information to be inspected. Specifically, when the user directly inputs an operation instruction to the control device 90 through the input unit 92, the input operation instruction may be any one of selecting a preset reagent amount, preset item information, or preset identification information on the display unit 91, or custom inputting the preset reagent amount, preset item information on the display unit 91. In response to any one of these preset information, the control device 90 directly acquires or indirectly acquires reagent-related information corresponding to such input operation instructions, for example: the amount of the reagent is preset.

In one example: as shown in fig. 3, when a display unit 91 displays a plurality of preset reagent amounts for a user to select one of the preset reagent amounts, or when the user directly displays a preset reagent amount by displaying a user-defined preset reagent amount through an input unit 92, the control device 90 directly obtains the preset reagent amount corresponding to the input operation instruction in response to the selection of the preset reagent amount or the user-defined input operation instruction. Of course, the user may preset in advance in the memory 95 based on different types of samples when presetting the volume of the reagent amount, for example: the preset reagent amount for the gynecological sample may be preset as: 400ul, 800ul, 1500ul, etc., the preset reagent amount for a sputum sample may be preset as: 200ul, 500ul, etc., and are not limited herein.

In another example, as shown in fig. 4, when a plurality of preset item information is displayed on the display unit 91 for the user to select one of the preset item information, or the user directly displays the user-defined item information on the display unit 91 through the input unit 92, in response to the selection of the item information or the user-defined input operation instruction, the control device 90 automatically maps the preset item information to a suitable preset reagent amount after acquiring the preset item information, and the control device 90 acquires the preset reagent amount again, which is the reagent amount corresponding to the indirectly acquired input operation instruction. For example: the display unit displays various selectable item information such as "dry chemistry+microscopy", "dry chemistry+microscopy+microecology", "dry chemistry", "microscopy", "microecology", etc., after a user selects or inputs a certain category of item on the display unit 91, the system will automatically map to a suitable preset reagent amount, for example, when the item information of "dry chemistry+microscopy" is selected, the system will automatically map to a preset reagent amount of 800ul, when the item information of "dry chemistry+microscopy+microecology" is selected, the system will automatically map to a preset reagent amount of 1500ul, etc., and in response to these preset item information, the control device 90 indirectly acquires the preset reagent amount corresponding to the input operation instruction of the item information. Of course, the user may preset in the memory 95 according to different types of samples when presetting the item information, for example: the gynecological sample preset item information can be preset as follows: and various selectable project information such as dry chemistry, microscopy, microecology, dry chemistry, microscopy, microecology and the like.

In another example, when a plurality of preset identification information is displayed on the display unit 91 for the user to select one of the preset identification information, or the user directly displays the customized identification information on the display unit 91 through the input unit 92, in response to the selection of the identification information or the customized input operation instruction, the control device 90 automatically maps the identification information to a suitable preset reagent amount after acquiring the identification information, and the control device 90 acquires the preset reagent amount again, which is indirectly to acquire the reagent amount corresponding to the input operation instruction. Or the identification information is automatically mapped to the appropriate item information, then the appropriate item information is matched to the appropriate preset reagent amount, and the control device 90 acquires the preset reagent amount again, which is still the reagent amount corresponding to the indirectly acquired input operation instruction. For example: the display unit 91 displays various selectable color identification information such as "red", "yellow", "green", etc., or various selectable symbol, text, etc., and after a user selects or inputs a certain category of these color identifications or symbol identifications, etc. on the display unit 91, the system will automatically map to a suitable preset reagent amount according to the association relationship preset in advance by the corresponding color identifications or symbol identifications, etc., or the system will automatically match to the suitable item information by the corresponding color identifications or coincidence identifications, etc. according to the association relationship preset in advance, and then match to the suitable preset reagent amount by the suitable item information, and in response to these preset identification information, the control device 90 indirectly acquires the preset reagent amount corresponding to the input operation instruction of the identification information. Of course, the identification information is not limited to a symbol, a character, a color, or the like, and any identification that can be distinguished is sufficient, and is not limited herein.

The control device 90 is further configured to obtain sample identity information of a biological sample on the attached matter and item association information of a target sample corresponding to the sample identity information. In some embodiments, as shown in fig. 6 and 7, the sample detection system 1000 further includes an identification device 100, where the identification device 100 is configured to identify sample identity information of a biological sample on an attachment, or the identification device 100 is configured to identify sample identity information of the biological sample on the attachment and item association information of a target sample corresponding to the sample identity information, and feed back the sample identity information of the target sample or the item association information of the target sample corresponding to the sample identity information to the control device 90, so that the control device 90 obtains the sample identity information of the biological sample on the attachment or the item association information of the sample identity information and the target sample corresponding to the sample identity information. The identification device 100 may be disposed in the sample detector or in other locations of the sample detection system 1000, for example, as shown in fig. 6 and 7, and is preferably disposed on or adjacent to a sample injection device (not shown) so as to uniformly manage the identity information and the multi-item information detection of multiple samples. The identification device 100 can identify not only sample identity information and item-related information, but also the presence or absence of a sample container, the type of sample container, and the like. The identification device 100 may be a code scanner, a camera, or the like. According to the sample identity information of the target sample or the sample identity information and the item association information of the target sample corresponding to the sample identity information, which are recognized by the recognition device 100, the control device 90 controls the corresponding action component to perform a corresponding action matched with the item association information on the target sample, for example: and controlling the sample detector to perform corresponding item detection on the identified target sample and obtain a detection result.

The item association information at least includes item information to be inspected. In some embodiments, the method further comprises a target reagent amount corresponding to the item information to be detected. For example: taking the gynecological leucorrhea sample as an example, when the identifying device 100 identifies that the item information to be detected corresponding to the target sample is a gynecological routine detection item and a microecological detection item, the item association information includes information of the gynecological routine detection item and the microecological detection item, and further preferably includes 1500ul of target reagent amounts corresponding to the gynecological routine detection item and the microecological detection item. The amount of target reagent corresponding to different items to be tested may be different. For example, taking a gynecological leucorrhea sample as an example, the detection items include various selectable item information such as "dry chemistry+microscopy", "dry chemistry+microscopy+microecology", "dry chemistry", "microscopy", "microecology", etc., and the memory 95 of the control device 90 stores corresponding preset data in advance, where the preset data information includes at least different target reagent amounts for different items or sub-categories of the same item, for example: the target eluent amount for the gynecological routine item is 800ul, the target eluent amount for the gynecological routine detection item and the microecological item is 1500ul, the target eluent amount for sputum is 1000ul, and the like. The item information to be detected and the corresponding target reagent amount can be in one-to-one correspondence, or in a many-to-one correspondence. For example: the gynecological routine items comprise a dry chemical detection item and a microscopic examination item, when the dry chemical and microscopic examination detection item is selected, the corresponding target eluent amount is 800ul, and when the microscopic examination detection item is selected, the corresponding target eluent amount is still 800ul, can also be 500ul, and the like, and can be input or selected by a user according to actual requirements.

After the above-mentioned reagent-related information and item-related information are obtained, the control device 90 compares the reagent-related information and the item-related information, and controls the elution device 20 to perform an elution operation on the target sample to form a sample liquid to be measured; the amount of the eluent corresponding to the eluting operation is a corresponding value of a small value in the reagent-related information and the item-related information, as shown in fig. 5A, 6 and 7. It will be appreciated that a "small value" means a minimum amount of reagent between the two, or an item corresponding to a minimum amount of reagent, or an identification corresponding to a minimum amount of reagent. The "corresponding value" is expressed as the amount of the reagent expressed directly or the amount of the reagent expressed after the indirect conversion. By way of example, a "small value" may be the smallest of two different amounts of reagent, such as: the minimum one of the preset and target reagent amounts is also the "small value" corresponding to the minimum one of the two different reagent amounts. The "small value" may also be a preset item or a to-be-detected item with the least amount of reagent corresponding to the preset item or the to-be-detected item, where the "small value" is the least amount of reagent corresponding to one of the preset item or the to-be-detected item; for example: the preset item is a dry chemical item, and the corresponding reagent amount is 300ul; the items to be detected are dry chemical, microscopic examination and microecology items, and the corresponding reagent amount is 1500ul; the small value is the "dry chemical item" and the corresponding value of the small value is 300ul. Of course, the small value may be the preset amount of the reagent represented by some of the identifiers, or the small value may be the least preset item or the item to be inspected of the preset amount of the reagent corresponding to one of the preset item and the item to be inspected represented by some of the identifiers. Likewise, the following "small reagent value" or "small item value" may be understood as the smallest amount of reagent, or as the preset item or item to be inspected corresponding to the smallest amount of reagent, or as the smallest amount of reagent characterized by some of the identifiers, or as the smallest amount of reagent corresponding to the item characterized by some of the identifiers.

In some embodiments, the elution device 20 may be disposed on or adjacent to a sample introduction device (not shown) so that the control device 90 uniformly schedules the processing sequences of different samples for sample management. Preferably, an elution device 20 is disposed at a position on the track of the sample introduction device to elute the sample coated on the sampling tool (e.g., flocked swab) in the sample container to the reagent, which may also be referred to as an eluent. In other embodiments, elution device 20 may be disposed within a sample detector. Wherein the elution device 20 is preferably placed in a sample detector. Taking the gynecological routine detector 50 as an example, the gynecological routine detector 50 is often provided with an incubation device (not shown) to incubate the sample to be tested to obtain the desired detection result of the corresponding tangible component or reaction structure, and this process cannot be performed too quickly or otherwise affects the activity of the tangible component or structure, and can only take a slow time. At this time, when the sample is incubated, the instrument has enough time to control the elution device 20 to complete the elution of other sample liquids to be tested due to the overlong incubation time, so as to improve the detection efficiency. In another embodiment, as shown in fig. 7, when the gynecological conventional detector 50 is a dry chemical detector 51 and a microscopic detector 52 separately, that is, the dry chemical detector 51 and the microscopic detector 52 are separate stand-alone devices, the dry chemical detector 51 is also preferentially arranged on the front of the sample detection system 1000, that is, the sample loading platform close to the sample feeding device, because the detection speed of the sample output from the dry chemical detector 51 is limited. Preferably, the elution device 20 is preferably positioned within the dry chemical detector 51 or near a location on the track prior to the dry chemical detector 51. In yet another embodiment, as shown in fig. 5A and fig. 6, when the sample detector is a gynecological conventional detector 50 and the gynecological conventional detector 50 includes a dry chemical detection device (not shown) and a microscopic detection device (not shown), that is, the dry chemical detection device and the microscopic detection device together form a detector, the elution device 20 may be disposed adjacent to the sample injection device or may be disposed in the detector; but is preferably located within the detector and adjacent to the sampling means (not shown) and/or the incubation means. In some embodiments, as shown in fig. 5B, the elution device 20 includes a liquid adding assembly (not shown) for adding a predetermined amount of the eluent to the sample solution, and a mixing assembly (not shown) for mixing the sample to be tested with the eluent added thereto to elute the gynecological sample from the collection tool into the solution. The mixing assembly can be any mode selected from stirring rod, eccentric mixing seat, and grip. Of course, not limited to gynecological specimens, other specimen types such as sputum, nasopharyngeal secretions, cervical secretions, and the like, which are required to be attached to the sampling tool after sampling, are contemplated as falling within the scope of the present application.

After obtaining the reagent related information and the item related information, the control device 90 compares the reagent related information and the item related information, and controls the eluting device 20 to perform an eluting operation on the target sample to form a sample liquid to be measured by using a corresponding value of the small value in the reagent related information and the item related information as an eluting liquid amount corresponding to the eluting operation. Therefore, the elution operation is carried out on the target sample with the lowest reagent dosage, the reagent dosage is fully saved, the detection cost is reduced, and the requirement of a user on flexible selection of different elution amounts is matched.

In some embodiments, when the reagent-related information is a preset reagent amount, the item-related information is to-be-detected item information and a target reagent amount corresponding to the to-be-detected item information; the step of comparing the reagent-related information with the item-related information and controlling the eluting device 20 to perform an eluting operation on the target sample to form a sample liquid to be measured includes: comparing the preset reagent amount with the target reagent amount to obtain a reagent small value; controlling the elution device 20 to perform an elution operation on the target sample to form a sample liquid to be measured; wherein the amount of the eluent corresponding to the elution operation is a small value of the reagent.

In another embodiment, when the reagent related information is preset item information and the item related information is item information to be detected, the step of comparing the reagent related information and the item related information, and controlling the eluting device 20 to perform an eluting operation on the target sample to form the sample liquid to be detected includes: comparing the preset project information with the project information to be detected to obtain a project small value; acquiring a corresponding reagent small value based on the project small value; wherein the amount of the eluent corresponding to the elution operation is a small value of the reagent.

In yet another embodiment, when the reagent association information is preset identification information and the item association information is item information to be inspected; the step of comparing the reagent-related information with the item-related information and controlling the eluting device 20 to perform an eluting operation on the target sample to form a sample liquid to be measured includes: acquiring a preset reagent amount based on preset identification information; acquiring a target reagent amount based on the information of the item to be detected; comparing the preset reagent amount with the target reagent amount to obtain a reagent small value; controlling the elution device 20 to perform an elution operation on a target sample to form a sample liquid to be measured; wherein the amount of the eluent corresponding to the elution operation is the small value of the reagent;

as described above, the identification information may be determined according to actual requirements, for example: after the user selects or inputs a certain category of color identifiers or symbol identifiers, etc. on the display unit 91, the system will automatically map to a proper preset reagent amount according to the preset association relationship in advance by the corresponding color identifiers or symbol identifiers, etc. or the system will automatically match to the proper project information according to the preset association relationship in advance by the corresponding color identifiers or coincidence identifiers, etc. and then match to the proper preset reagent amount by the proper project information, and in response to the preset identifier information, the control device 90 indirectly obtains the preset reagent amount corresponding to the input operation instruction of the identifier information.

In other embodiments, when the reagent related information is the preset identification information and the item related information is the item information to be detected; the step of comparing the reagent-related information with the item-related information and controlling the eluting device 20 to perform an eluting operation on the target sample to form a sample liquid to be measured includes: acquiring preset item information based on preset identification information; comparing the preset project information with the project information to be detected to obtain a project small value; acquiring a corresponding reagent small value based on the project small value; controlling the elution device 20 to perform an elution operation on the target sample to form a sample liquid to be measured; wherein the amount of the eluent corresponding to the elution operation is a small value of the reagent. Namely, the difference of the items is compared to obtain small reagent values corresponding to less items, and the elution operation is performed.

In still another embodiment, when the reagent-related information is preset identification information, the item-related information includes item information to be inspected and a target reagent amount corresponding based on the item information to be inspected; the step of comparing the reagent-related information with the item-related information and controlling the eluting device 20 to perform an eluting operation on the target sample to form a sample liquid to be measured includes: acquiring a preset reagent amount based on preset identification information; comparing the preset reagent amount with the target reagent amount to obtain a reagent small value; controlling the elution device 20 to perform an elution operation on the target sample to form a sample liquid to be measured; wherein the amount of the eluent corresponding to the elution operation is a small value of the reagent.

Also, as described above, the system may automatically map to the appropriate preset reagent amount by the corresponding color identifier or symbol identifier or the like according to the association relationship preset in advance, or the system may automatically match to the appropriate item information by the corresponding color identifier or symbol identifier or the like according to the association relationship preset in advance, and then match to the appropriate preset reagent amount by the appropriate item information. In response to these preset identification information, the control device 90 indirectly acquires such preset amounts of the reagents corresponding to the input operation instructions of the identification information. And the control device 90 compares the obtained target reagent amount with the preset reagent amount, and takes the reagent addition amount of a small value to perform the elution operation, so as to maximize the cost saving.

Or, when the reagent association information is preset identification information, the item association information comprises to-be-detected item information and a target reagent amount corresponding to the to-be-detected item information; the step of comparing the reagent-related information with the item-related information and controlling the eluting device 20 to perform an eluting operation on the target sample to form a sample liquid to be measured includes: acquiring preset item information based on preset identification information; comparing the information quantity of the preset project with the information of the project to be detected to obtain a project small value; acquiring a corresponding reagent small value based on the project small value; controlling the elution device 20 to perform an elution operation on the target sample to form a sample liquid to be measured; wherein the amount of the eluent corresponding to the elution operation is a small value of the reagent.

As described above, when the eluting device 20 performs the eluting operation on the target sample with the small value corresponding to the small value to form the sample liquid to be tested, although different suitable reagent amounts can be selected according to the actual requirement of the user by matching different detection samples, and reagents can be saved. However, the existence of insufficient liquid amount can not support all items to be inspected for which the target sample is finished, so that the instrument alarms due to insufficient liquid amount, for example: the insufficient suction alarm or the phenomenon of suction appears, influences the accuracy of testing result and even damages the instrument. In order to solve the problem, the application further provides a scheme for automatically judging and realizing the shortage of liquid amount by the system.

In some embodiments, the sample testing system 1000 further comprises a fluid replacement device 10, the control device 90 being connected to the fluid replacement device 10, the control device 90 further being configured to: when the small value is the reagent related information, determining and controlling the fluid infusion device 10 to conduct fluid infusion operation on the sample fluid to be tested; when the small value is the item-related information, or when the reagent-related information and the item-related information are the same, it is determined that the fluid replacement device 10 is controlled not to perform fluid replacement operation on the sample fluid to be tested.

As shown in fig. 5B, 6 and 7, the fluid infusion device 10 may be disposed in the sample testing device, or may be disposed at another location of the sample testing system 1000, for example, as shown in fig. 5B, 6 and 7, and preferably disposed in the sample testing device so as to be combined with other reagent injection mechanisms into an integrated mechanism or separately managed so as to simplify the control sequence. The fluid infusion device 10 may be a needle assembly and fluid circuit system, or may be a solution for directly transporting and dispensing a reagent through a pipeline, and the like, which are all within the scope of the present application. The fluid infusion device 10 may be combined with the first sample information detection device 30 described below to form the same device, or the fluid infusion device 10 and the first dilution device 40 described below may be combined with each other to form the same device, as shown in fig. 5B, which falls within the scope of the present application.

In some embodiments, the control device 90 compares the reagent-related information with the item-related information, and determines to control the fluid replacement device 10 to perform the fluid replacement operation on the sample fluid to be tested when the compared small value is the reagent-related information. In this case, the amount of the reagent selected or automatically inputted by the user is insufficient to match the target amount of the reagent required for the item to be tested, so that the fluid replacement operation is required. Further, the reagent addition amount corresponding to the fluid replacement operation is a difference value corresponding value between the associated information and the item associated information. As explained above, the "difference corresponding value" may be either a difference between the amounts of the reagents, a difference between the amounts of the reagents corresponding to the difference between the items, a difference between the amounts of the reagents represented by the identification information, a difference between the items represented by the identification information, or a difference between the items corresponding to the difference between the items, or the like, and is within the scope of the present invention as long as it is finally embodied as the amount of the reagent added, whether directly displayed or indirectly fed back or converted.

Preferably, when the reagent related information is a preset reagent amount, if the item related information is the item information to be detected and the target reagent amount corresponding to the item information to be detected, the preset reagent amount is directly compared with the target reagent amount, and if the small value corresponding value is the preset reagent amount, the control device 90 determines that the liquid replenishing device 10 performs the liquid replenishing operation on the sample liquid to be detected; when the target reagent amount is equal to or smaller than the preset reagent amount, the control device 90 determines to control the liquid replenishing device 10 not to perform the liquid replenishing operation on the sample liquid to be tested, that is, the liquid replenishing operation on the sample is regarded as an "idle state". Or when the reagent related information is the preset reagent amount, and when the item related information is the item information to be detected, the control device 90 firstly obtains the corresponding target reagent amount based on the item information to be detected, then compares the preset reagent amount with the target reagent amount, and if the preset reagent amount is the small value, the control device 90 determines that the liquid replenishing device 10 performs the liquid replenishing operation on the sample liquid to be detected; when the target reagent amount is equal to or smaller than the preset reagent amount, the control device 90 determines to control the liquid replenishing device 10 not to perform the liquid replenishing operation on the sample liquid to be tested, that is, the liquid replenishing operation on the sample is regarded as an "idle state". Further preferably, the reagent addition amount corresponding to the liquid replenishing operation at this time is a corresponding value of the absolute value of the difference between the preset reagent amount and the target reagent amount.

Preferably, when the reagent related information is preset item information, and when the item related information is item information to be detected and a target reagent amount corresponding to the item information to be detected, the control device 90 firstly obtains a corresponding preset reagent amount based on the preset item information, then compares the preset reagent amount with the target reagent amount, and if the preset reagent amount is a small value, the control device 90 determines that the liquid replenishing device 10 performs liquid replenishing operation on the sample liquid to be detected; when the target reagent amount is equal to or smaller than the preset reagent amount, the control device 90 determines to control the liquid replenishing device 10 not to perform the liquid replenishing operation on the sample liquid to be tested, that is, the liquid replenishing operation on the sample is regarded as an "idle state". Or when the reagent related information is preset item information, and when the item related information is item information to be detected, the control device 90 compares the preset item with the item to be detected, and if the small value is the preset item, that is, the reagent amount corresponding to the preset item is smaller than the reagent amount corresponding to the item to be detected, the control device 90 determines that the liquid supplementing device 10 carries out liquid supplementing operation on the sample liquid to be detected; when the small value is the item to be inspected, or when the preset item and the item to be inspected are the same, the control device 90 determines to control the fluid infusion device 10 not to perform the fluid infusion operation on the sample fluid to be inspected, that is, the fluid infusion operation on the sample is regarded as an "idle state". Further preferably, the reagent addition amount corresponding to the fluid infusion operation at this time is a corresponding value of the absolute value of the difference between the preset reagent amount corresponding to the preset item and the target reagent amount corresponding to the item to be tested. Or the reagent addition amount corresponding to the liquid supplementing operation is the reagent amount corresponding to the difference item between the preset item and the item to be detected.

Preferably, when the reagent association information is preset identification information, and when the item association information is to-be-detected item information or is to-be-detected item information and a target reagent amount corresponding to the to-be-detected item information, the control device 90 matches a suitable preset reagent amount based on the preset identification information, or matches the suitable item information and then matches the suitable preset reagent amount based on the suitable item information. Then comparing the preset reagent amount with the target reagent amount, and if the small value is the preset reagent amount, determining that the liquid supplementing device 10 carries out liquid supplementing operation on the sample liquid to be tested by the control device 90; when the target reagent amount is equal to or smaller than the preset reagent amount, the control device 90 determines to control the liquid replenishing device 10 not to perform the liquid replenishing operation on the sample liquid to be tested, that is, the liquid replenishing operation on the sample is regarded as an "idle state". Or, comparing the preset item with the item to be detected later, if the small value is the preset item, that is, the reagent amount corresponding to the preset item is smaller than the reagent amount corresponding to the item to be detected, the control device 90 determines that the liquid replenishing device 10 performs liquid replenishing operation on the sample liquid to be detected; when the small value is the item to be inspected, or when the preset item and the item to be inspected are the same, the control device 90 determines to control the fluid infusion device 10 not to perform the fluid infusion operation on the sample fluid to be inspected, that is, the fluid infusion operation on the sample is regarded as an "idle state". At this time, the reagent addition amount corresponding to the fluid infusion operation is a corresponding value of the absolute value of the difference between the preset reagent amount corresponding to the preset item and the target reagent amount corresponding to the item to be detected. Or the reagent addition amount corresponding to the liquid supplementing operation is the reagent amount corresponding to the difference item between the preset item and the item to be detected.

In some embodiments, after ensuring that the sample liquid to be tested is sufficient, the control device 90 is further configured to perform detection corresponding to the item association information on the sample liquid to be tested, and obtain a corresponding detection result. After determining to perform the fluid infusion operation and obtaining the actual detection result, the control device 90 amplifies the detection result according to the reagent addition amount corresponding to the fluid infusion operation, so as to ensure the true and accurate result.

In some embodiments, the biological sample is not just the gynecological leucorrhea sample exemplified above, but may be other samples. Further preferably, the biological sample is selected from any one of sputum, nasopharyngeal secretion, cervical secretion, vaginal secretion. The solution to be tested, which is formed after the sample attached to the sampling tool is eluted, may result in insufficient solution volume to support the sample as it is done for all items to be tested, is suitable for use in this aspect of the application.

In summary, the above-mentioned scheme has solved present sample check out test set can not match the demand that different sample detected items should select different eluent amounts, has saved reagent cost, has especially solved the eluent amount of single setting at present and probably brought can not support the sample to be measured to finish the detection of all items, even consequently can lead to the instrument in-process of detecting alert even harm instrument's problem. In addition, the automatic elution mode of the current instrument is also very likely to cause poor elution effect, and the scheme helps a user to flexibly select the amount of the eluent corresponding to different detection items of different samples according to own experience, so that the elution effect is further ensured, and the accurate determination of the detection result is improved. The sample detection system 1000 provided by the application can be matched with different detection samples according to the actual demands of users to select different suitable reagent amounts, and has high degree of automation and enhances the user experience.

Currently, when a doctor takes a sample of a patient, for example: gynaecological leucorrhea, cervical samples, sputum samples and the like, the individuals of patients have differences or the doctors manually operate the patients have deviations, so that the acquired sample amounts have differences. Therefore, in the sample eluting process, the quantitative eluent is added and/or the liquid amount is replenished, so that the concentration of each sample is different and changed, and uncertainty exists, and the detection result of the final detection item is affected if the concentration of the sample is not satisfied. Accordingly, the present application also provides a scheme for detecting the concentrations of different samples by setting the sample information detecting means and automatically determining whether to perform different dilution operations for different detection items.

As shown in fig. 5B, 5C, 6 and 7, in some embodiments, the sample detection system 1000 further comprises a first sample information detection device 30, and the control device 90 is connected to the first sample information detection device 30, and the control device is further configured to:

controlling the first sample information detecting means 30 to perform a detection operation of concentration information on the sample liquid to be detected;

receiving concentration information of a sample liquid to be detected;

judging whether the concentration information meets a threshold range or not to obtain a judging result;

And determining whether to execute the dilution operation according to the judgment result.

As shown in fig. 5B, 5C, 6 and 7, in some embodiments, the first sample information detecting device 30 is configured to detect that the sample liquid to be detected performs a detection operation of concentration information. The first sample information detection device 30 is disposed within the sample detector or adjacent to the sample introduction device. When the sample is a leucorrhea sample, the sample detector includes the gynecological conventional detector 50 and the microecological detector 70, and the gynecological conventional detector 50 has long sample detection time and higher sample dilution requirement, the first sample information detecting device 30 is more preferably disposed in the gynecological conventional detector 50 or near the gynecological conventional detector 50. This is because in the micro-ecological detector 70, the required sample concentration is high, for example, in the emergency situation, the user may directly perform dry smear on the sample collected by the collecting tool to make a sheet, and the prepared dry sheet is directly loaded to the micro-ecological detector 70 to perform micro-ecological project detection, so that the sample is not required to be eluted and the concentration information of the liquid to be detected is detected; however, in the gynecological conventional detector 50, the concentration of the sample is high, so that the concentration of the sample is too dense to be easily identified, and the accuracy of the detection result is lowered, so that the concentration information needs to be eluted and then detected to determine whether to dilute the sample. After the sample to be measured in the sample container is eluted to form a sample liquid to be measured, a sampling device (not shown) of the gynecological conventional detector 50 collects the sample liquid to be measured in the sample container and transfers it to the first sample information detection device 30, and the sampling device is preferably disposed in each detector, but may also be mounted on a track of the sampling device. The first sample information detecting device 30 includes a housing unit that receives the sample liquid to be detected to which the sampling device is transferred, and a photoelectric detecting unit that detects the sample liquid to be detected, which is provided at a peripheral side of the housing unit. Further preferably, the photodetection unit comprises a transmitter and a receiver, for example: a laser module and a detector. The first sample information detecting means 30 characterizes the concentration information of the sample liquid to be measured with a PD value. Concentration information is a broad concept and can be understood as mass concentration, turbidity, etc. Illustratively, it is preferred that the turbidity information of the sample fluid to be measured is characterized by a PD value. The PD value refers to the light intensity value received by the detector, and when a scattering method is used, the PD value is positively correlated with the turbidity, namely the larger the PD value is, the larger the turbidity is, which means that the higher the sample content in the sample liquid to be detected is; when using the transmission method, the PD value is inversely related to the turbidity, i.e. the higher the PD value, the lower the turbidity, which means that the lower the sample content in the sample liquid to be measured. By way of example, the detection of PD by transmission is taken as an example hereinafter, although the invention is not limited thereto.

In some embodiments, the control device 90 obtains the concentration information of the sample liquid to be tested, and determines whether the concentration information meets the threshold range, so as to obtain a determination result; determining whether to perform the diluting operation according to the judgment result further includes the steps of:

when the concentration of the sample liquid to be measured, which is represented by the concentration information, is judged to be too high, the dilution operation is determined to be executed;

obtaining a dilution amount corresponding to the dilution operation;

the dilution operation is performed based on the dilution amount.

In the above-described step, when it is determined to perform the dilution operation, it is preferable to perform the dilution operation based on the dilution amount using the fluid replacement device 10. Further preferably, in this case, the control device 90 may control the fluid replacement device 10 to perform the fluid replacement operation corresponding to the addition amount of the reagent at one time, based on the combined determination of whether the fluid replacement operation is to be performed and whether the dilution operation is to be performed, and when both the determinations are yes, that is, when both the fluid replacement operation and the dilution operation are to be performed. That is, when the dilution operation and the fluid replacement operation are controlled to be performed, the reagent addition amount of the fluid replacement device 10 is the sum of the dilution amount corresponding to the dilution operation and the fluid replacement amount corresponding to the fluid replacement operation. At this time, the control device 90 amplifies the detection result based on the total reagent addition amount to ensure the true and accurate detection result.

As shown in fig. 5A to 8, in another embodiment, the sample detection system 1000 further includes a first dilution device 40, the control device is connected to the first dilution device 40, and the control device 90 is further configured to: when determining to execute the dilution operation, obtaining the dilution amount corresponding to the dilution operation; the first dilution means 40 is controlled to perform a dilution operation based on the dilution amount. Preferably, the first dilution means 40 may be provided within the sample detector or adjacent to the sample introduction means.

As shown in fig. 7, taking a gynecological leucorrhea sample as an example, in some embodiments, when the sample detector includes at least a gynecological routine detector 50, when the gynecological routine detector 50 is a dry chemical detector 51 and a microscopic detector 52 that are separately provided, that is, the dry chemical detector 51 and the microscopic detector 52 are separate stand-alone instruments, the dry chemical detector 51 and the microscopic detector 52 are respectively provided with the first dilution device 40 correspondingly. I.e. the first dilution means 40 comprises a first dry dilution means 41 and a first microscopic dilution means 42. This allows the concentration information of the target sample to be detected by the unique first sample information detecting means 30 and fed back in different sample detectors and determines whether a dilution operation needs to be performed or not, so as to ensure that the sample concentration of each detector meets the detection requirements of different items. Further preferably, when the sample detector includes a dry chemical detector 51 and a microscopic chemical detector 52, the control device 90 is further configured to: when it is determined that the liquid replenishing device 10 is controlled to perform the liquid replenishing operation on the sample liquid to be measured, the dry liquid replenishing operation is performed on the sample liquid to be measured transferred to the dry chemical detector 51 and the microscopic liquid replenishing operation is performed on the sample liquid to be measured transferred to the microscopic detector 52. At this time, the first dry chemical diluting device 41 and the first microscopic diluting device 42 may be used to perform corresponding dry chemical fluid infusion operation and microscopic chemical fluid infusion operation on the sample fluid to be tested, that is, the fluid infusion device 10 is the first dry chemical diluting device 41 and the first microscopic chemical diluting device 42 respectively corresponding to each other. In other embodiments, the fluid infusion device 10, the first dry chemical dilution device 41, and the first microscopic dilution device 42 may be different, and the number of fluid infusion devices 10 may be multiple, for example: as shown in fig. 7, the dry chemical detector 51 and the microscopic inspection detector 52 are each provided with a fluid infusion device 10, so that the dilution device and the fluid infusion device 10 are separately and independently provided, and the detection efficiency can be improved. Of course, the detection result of the dry chemical project and the detection result of the microscopic examination project obtained finally at this time are both determined whether to amplify the detection result of the respective projects based on whether to perform the dilution operation and whether to perform the fluid replacement operation.

In some embodiments, as shown in fig. 5B and 5C, fig. 5B is a partially enlarged view of the gynecological line portion structure a in fig. 5A, that is, a partially enlarged view of the first eluting device 20, the first sample information detecting device 30, the first diluting device 40 and the fluid compensating device 10, and fig. 5C is a cross-sectional view of the first sample information detecting device 30 (labeled "B" in the drawings) in fig. 5B. As shown in fig. 5B, 6 and 7, the fluid infusion device 10 may be disposed in the sample testing device, or may be disposed at another location of the sample testing system 1000, for example, as shown in fig. 5B, 6 and 7, and preferably disposed in the sample testing device so as to be combined with other reagent injection mechanisms into an integrated mechanism or separately managed so as to simplify the control sequence. The fluid infusion device 10 may be a needle assembly and fluid circuit system, or may be a solution for directly transporting and dispensing a reagent through a pipeline, and the like, which are all within the scope of the present invention. The fluid infusion device 10 may be combined with the first sample information detection device 30 to form a single device, or the fluid infusion device 10 and the first dilution device 40 may be combined with each other to form a single device, as shown in fig. 5B, which falls within the scope of the present invention.

In some embodiments, as shown in fig. 7, fig. 7 shows that the fluid infusion device 10 and the first dry chemical dilution device 41 in the dry chemical detector 51 are different devices, and the fluid infusion device 10 and the first microscopic dilution device 42 in the microscopic examination detector 52 are the same device. When the sample detector at least comprises the gynecological routine detector 50, when the gynecological routine detector 50 is a dry chemical detector 51 and a microscopic detector 52 which are separately arranged, that is, the dry chemical detector 51 and the microscopic detector 52 are independent single-machine instruments respectively, the fluid infusion amount corresponding to the dry chemical fluid infusion operation is smaller than the fluid infusion amount corresponding to the microscopic fluid infusion operation. The method combines the particularity of the gynecological leucorrhea sample, has higher requirement on the concentration of the dry sample, and can fully react with the dry sheet reagent to obtain a better detection result only when the fluid replacement amount is small. However, the microscopic examination is a wet-film microscopic examination, most samples are wet-stained, and the increase of the fluid infusion amount is also helpful for avoiding or reducing the defects of unclear overlapping view or inaccurate counting of substances such as cells under microscopic photographing.

In yet another embodiment, as shown in fig. 5B and fig. 6, when the gynecological conventional detector 50 is formed by combining a dry chemical detector 51 and a microscopic detector 52, that is, when the dry chemical detector 51 and the microscopic detector 52 together form one detector, that is, when the dry chemical detector 51 and the microscopic detector 52 are only the dry chemical detector and the microscopic detector respectively corresponding to each other in one detector, only one first dilution device 40 is provided. The first dilution device 40 includes a fluid circuit assembly and a dilution tank. The dilution tank receives the sample liquid to be measured transferred to by the sampling device and performs a first dilution operation under the matching instruction of the control device 90. Illustratively, the diluent circuit assembly may be a diluent needle assembly and diluent delivery circuit, or diluent may also be delivered directly from the circuit assembly to the diluent reservoir. It will be appreciated that the dilution may be performed directly at the reaction site or even at the detection site without the need for a dilution tank, for example, when the first dilution means 40 is provided in the dry chemical detector 51, the dilution may be added directly at the reaction site of the incubation means; a dilution tank may be separately provided, and after the addition of the diluent is completed, the diluted sample solution to be tested is added to the carrying component on the reaction site, for example: reagent test cards or slides, etc. Further preferably, the fluid infusion device 10 and the first dilution device 40 may be the same device, for example: the gynecological routine detector 50 has only one fluid infusion device 10 therein, and the fluid infusion device 10 is used for both fluid infusion when fluid infusion is required and dilution when dilution is required, which is advantageous in terms of structural simplicity. Of course, the dry chemical detection result and the microscopic examination result obtained at this time are both determined whether to amplify the detection result of the item based on whether the sample liquid to be measured corresponding to the corresponding item is subjected to the dilution operation and the liquid replenishment operation. That is, when the control device 90 determines to perform the dilution operation and the fluid replacement operation, the reagent addition amount of the fluid replacement device 10 is the sum of the dilution amount corresponding to the dilution operation and the fluid replacement amount corresponding to the fluid replacement operation. The amplification process is also performed in accordance with the amount of the reagent added. It should be understood that, although the fluid infusion device 10 and the first dilution device 40 in fig. 6 are the same device, the fluid infusion device 10 and the first dilution device 40 may not be the same device, i.e., the fluid infusion device 10 and the first dilution device 40 may be separately provided in the apparatus.

Still taking the gynecological leucorrhea sample as an example, in some embodiments, as shown in fig. 5A, 6 and 7, the sample detector comprises at least a sample detector comprising a microecological detector 70. The micro-ecological detector 70 may be a plurality of devices or one device. In some embodiments, the micro-ecological detector 70 includes a pushing device 71 and a reading device 72, the pushing device 71 is used for making a sheet of the sample liquid to be detected to obtain a dry sheet, and the reading device 72 is used for scanning the dry sheet prepared by the pushing device 71 to obtain a micro-ecological detection result. The pushing device 71 includes a sampling unit, a liquid path unit, and a pushing unit, where the sampling unit is used to collect the sample liquid to be tested in the eluted sample container, the liquid path unit is used to complete cleaning, reagent supply, driving, and the pushing unit is used to push the sample liquid to be tested on the slide into a preset shape. The slide pushing device 71 may further set a staining unit according to actual requirements to stain the sample liquid to be measured, and send the sample liquid to the slide pushing unit to push the slide, or transfer the slide coated with the sample liquid to be measured to the staining unit to stain the slide after the slide pushing is completed. The prepared dry sheet is unloaded from the sheet pushing device 71 and transferred to the sheet reading device 72. The film reading device 72 comprises a sample feeding unit, a sample discharging unit and a micro scanning unit, wherein the sample feeding unit receives a dry film of a sample to be detected from manual sample feeding or a dry film transferred to the film pushing device 71, and sends the dry film to the micro scanning unit for film reading so as to obtain a micro ecological detection result. The pusher 71 and the reader 72 may be separate devices or integrated devices. In the case of a single instrument, the pusher 71 and reader 72 may be connected via rails as described above, or via other transfer modules, such as: a robotic arm, etc., preferably a rail.

In some embodiments, when the sample detector includes at least the sample detector including the micro-ecological detector 70, the micro-ecological detector 70 includes a pushing device 71 and a reading device 72, where the pushing device 71 and the reading device 72 may be a separate instrument or an integrated same instrument. In the case of a single device, the fluid infusion device 10 is preferably disposed within the pusher device 71. When integrated into the same instrument, the fluid infusion device 10 is preferably positioned adjacent to the pusher device 71 for fluid infusion and pusher operation.

Preferably, when the sample detector comprises at least the sample detector comprising the micro-ecological detector 70, the sample detection system 1000 further comprises an aggregation device 73, wherein the control device 90 is connected to the aggregation device 73 and the micro-ecological detector 70, and the control device 90 is further configured to: when it is determined that the concentration of the sample liquid to be measured, which is represented by the concentration information, is too low, the aggregation device 73 is controlled to perform an aggregation operation on the sample liquid to be measured transferred to the micro-ecological detector 70.

Further preferably, the sample detection system 1000 further comprises a second sample information detection device 80. Similar to the first sample information detecting device 30, the second sample information detecting device 80 is also used to detect concentration information of the sample liquid to be measured. When the user selects to use only the micro-ecological sensing instrument 70 through the input unit 92 of the control device 90; or the user disconnects the micro-ecological detector 70 and the gynecological routine detector 50 directly, for example by means of an instrument switch control button; or the control device 90, when recognizing that the sample information is only required to be detected by the microecological item, for example: when the identification device 100 scans the bar code to identify, the control device 90 only controls the micro-ecological detector 70 to detect micro-ecological items of the sample liquid to be detected, at this time, whether the sample liquid to be detected is manually loaded or transported to the sample container at the micro-ecological detector 70 from the sample container, the second sample information detection device 80 detects the concentration information of the sample liquid to be detected of the eluted sample container, and when the detected concentration information does not meet the threshold range, that is, when the concentration information represents that the concentration of the sample liquid to be detected is too high or too low, the display unit 91 of the control device 90 prompts the abnormal information; the too low control device 90 triggers the start-up aggregation device 73 to aggregate the sample liquid to be tested, and the too high control device performs the dilution operation on the collected sample liquid to be tested.

Further preferably, as shown in fig. 6 and 7, the sample detection system 1000 also preferably includes a second dilution device 60. The second diluting device 60 performs a second diluting operation on the sample liquid to be measured. The second dilution device 60 is disposed within the micro-ecological detector 70 or adjacent to the sample injection device. In some embodiments, the micro-ecological detector 70 may be a plurality of devices connected or may be a single device. In another embodiment, as shown in fig. 6 and 7, the micro-ecological detector 70 includes a pushing device 71 and a reading device 72, where the pushing device 71 and the reading device 72 may be a single instrument or an integrated same instrument. In the case of separate instruments, the second dilution means 60 is preferably arranged in the blade pushing means 71, i.e. the read-out means 72 is not provided with the second dilution means 60. When integrated into the same instrument, the second dilution means 60 is also integrated into the instrument. It will be appreciated that the first dilution unit 40 and the second dilution unit 60 may be of substantially the same or different composition. For example, the second dilution device 60 may be integrated with the mixing unit in the blade pushing device 71, i.e. the dilution assembly may be a liquid path assembly such as a pipeline or a needle assembly, for directly adding the diluent to the mixing unit to perform dilution and mixing. When the detected concentration information does not meet the threshold range, that is, when the concentration information represents that the concentration of the sample liquid to be detected is too high or too low, the display unit 91 of the control device 90 prompts the abnormal information; the control device 90 triggers the start-up aggregation device 73 to aggregate the sample liquid to be tested when the control device is too low, and controls the second dilution device 60 to perform dilution operation on the collected sample liquid to be tested when the control device is too high. In some embodiments, the fluid infusion device 10 and the second dilution device 60 are different, and the number of fluid infusion devices 10 may be plural, for example: the micro-ecological detector 70 is provided with the liquid supplementing device 10 and the second diluting device 60, so that the second diluting device 60 and the liquid supplementing device 10 are separately and independently arranged, and the detection efficiency can be improved. Of course, the detection result of the finally obtained microecological item at this time is determined whether to amplify the detection result of the item based on whether to perform the dilution operation and whether to perform the fluid replacement operation. In other embodiments, the fluid replacement device 10, the second dilution device 60 may be the same device, such as: the micro-ecological detector 70 has only one fluid infusion device 10, and the fluid infusion device 10 is used for both fluid infusion when fluid infusion is required and dilution when dilution is required, which is beneficial to the structural simplification. Of course, the detection result of the finally obtained microecological item at this time is determined whether to amplify the detection result of the item based on whether to perform the dilution operation and whether to perform the fluid replacement operation. That is, when the control device 90 determines to perform the dilution operation and the fluid replacement operation, the reagent addition amount of the fluid replacement device 10 is the sum of the dilution amount corresponding to the dilution operation and the fluid replacement amount corresponding to the fluid replacement operation. The amplification process is also performed in accordance with the amount of the reagent added.

Further preferably, as shown in fig. 6 and 7, the aggregation device 73 is provided in the micro-ecological sensor 70 or near the outside of the micro-ecological sensor 70, preferably in the micro-ecological sensor 70. When the concentration information detected by the first sample information detecting means 30 or the second sample information detecting means 80 characterizes that the concentration of the sample liquid to be measured is too low, the control means 90 controls the aggregating means 73 to perform an aggregating operation on the sample liquid to be measured to increase the concentration of the sample liquid to be measured thereof. The aggregation device may be a centrifuge or a settler, preferably a centrifuge. For example, when the aggregation device 73 is a centrifuge, the sampling device transfers a proper amount of the eluted sample liquid to be tested into a centrifuge tube, and then the centrifuge tube is centrifuged, the supernatant liquid after centrifugation and delamination is discarded, and the concentrated liquid after aggregation of the lower layer is taken out and transferred onto a slide for a slide pushing operation. Preferably, in order to further remove impurities in the sample liquid, a gradient separation liquid can be added into the centrifuge tube, the sample liquid to be tested is layered under the action of the gradient separation liquid, the upper liquid is discarded, and the upper liquid is left and is centrifuged along with the centrifuge tube. Also for example: when the aggregation device 73 is a settler, the settler includes a settling tube and an empty slide inserted into the bottom of the settling tube, and the settling tube may include a filter membrane or no filter membrane. And transferring the sample liquid to be tested into a sedimentation cup by a sampling device, and carrying out sedimentation by utilizing the gravity action or a negative pressure film type so that the sample liquid to be tested gradually settles on an empty glass slide to form an aggregated sample liquid. At this time, the slide pushing operation may be performed to satisfy the preset shape if the sample shape on the slide is determined not to satisfy the preset shape, and the control device 90 sets the standard of the corresponding preset shape in advance for identifying and determining whether the standard of the preset shape is satisfied.

In some embodiments, as shown in fig. 5A and 6 and 7, the gynecological line is configured when the sample detector includes at least a gynecological conventional detector 50 and a microecological detector 70. The gynecological routine detector 50 is used for performing gynecological routine item detection on a target sample, and the microecological detector 70 is used for performing microecological item detection on the target sample. Further preferably, when forming the pipeline, the control device 90 is further configured to: when it is determined to control the fluid replacement device 10 to perform the fluid replacement operation on the sample fluid to be measured, which is transferred to the gynecological conventional detector 50, and/or to perform no fluid replacement operation on the sample fluid to be measured, which is transferred to the microecological detector 70. This is because the micro-ecological detector 70 needs to make a sheet and then scan and analyze a dry sheet, and the sample concentration is required to be high. At present, the user end does not even perform elution, directly coats the sample of the sampling tool on an empty slide, and then scans the slide. Therefore, to ensure the high concentration requirement of the sample by the micro-ecological detector 70, even if the control device 90 determines that the fluid infusion device 10 is to be controlled to perform fluid infusion operation on the sample fluid to be tested, the control device 90 does not perform fluid infusion operation on the sample fluid to be tested that is moved to the micro-ecological detector 70, that is, even if the second dilution device 60 and/or the fluid infusion device 10 are provided near or in the micro-ecological detector 70, the second dilution device 60 and/or the fluid infusion device 10 does not perform fluid infusion operation on the sample fluid to be tested. The second dilution device 60 and/or the fluid replacement device 10 disposed near the micro-ecological monitor 70 or within the micro-ecological monitor 70 in the gynecological line is often used to perform a second dilution operation on the sample fluid to be measured. Further preferably, when the control device 90 determines that the fluid replacement device 10 is to be controlled to perform fluid replacement operation on the sample fluid to be tested, and the fluid to be tested transferred to the micro-ecological detector 70 is not to be subjected to fluid replacement operation, the control device 90 preferably ensures the sample fluid volume of the micro-ecological detector 70, and supplements the sample fluid to be tested in the gynecological routine program. For example, when there is 800ul of the sample solution to be tested, and it is determined that 700ul of the solution needs to be replenished after the determination, the control device 90 preferably ensures 500ul of the sample solution to be tested of the micro-ecological detector 70, and adds 700ul of the solution to be replenished to 300ul of the sample solution to be tested of the gynecological conventional detector 50. Further, when the gynecological routine detector 50 is a dry chemical detector 51 and a microscopic detector 52 which are separately provided, that is, the dry chemical detector 51 and the microscopic detector 52 are independent single machines, the fluid infusion amount corresponding to the dry chemical fluid infusion operation is smaller than the fluid infusion amount corresponding to the microscopic fluid infusion operation. For example: the gynecological routine detector 50 requires a total of 700ul of fluid replacement, and the dry chemical fluid replacement operation corresponds to 200ul of fluid replacement and the microscopic fluid replacement operation corresponds to 500ul of fluid replacement. The method combines the particularity of the gynecological leucorrhea sample, has higher requirement on the concentration of the dry sample, and can fully react with the dry sheet reagent to obtain a better detection result only when the fluid replacement amount is small. However, the microscopic examination is a wet-film microscopic examination, most samples are wet-stained, and the increase of the fluid infusion amount is also helpful for avoiding or reducing the defects of unclear overlapping view or inaccurate counting of substances such as cells under microscopic photographing. In yet another embodiment, when the gynecological conventional detector 50 is formed by combining a dry chemical detector 51 and a microscopic detector 52, that is, when the dry chemical detector 51 and the microscopic detector 52 together form a single detector, only one fluid infusion device 10 and/or the first dilution device 40 is provided, and the fluid infusion operation is performed by the fluid infusion device 10 or the first dilution device 40, for example: and 700ul of fluid infusion. It will be appreciated that the fluid infusion device 10, the first dilution device 40 may be the same device, such as: only one fluid infusion device 10 is arranged in the gynecological routine detector 50, and the fluid infusion device 10 is used for both fluid infusion operation when fluid infusion operation is required to be performed and dilution operation when dilution operation is required to be performed, so that the structure is simplified; or the gynecological routine detector 50 is provided with both a fluid infusion device 10 and a first dilution device 40, wherein the fluid infusion device 10 is used for executing fluid infusion operation, and the first dilution device 40 is used for executing dilution operation. Of course, the dry chemical detection result and the microscopic examination result obtained at this time are both determined whether to amplify the detection result of the item based on whether the sample liquid to be measured corresponding to the corresponding item is subjected to the dilution operation and the liquid replenishment operation. That is, when the control device 90 determines to perform the dilution operation and the fluid replacement operation, the reagent addition amount of the fluid replacement device 10 is the sum of the dilution amount corresponding to the dilution operation and the fluid replacement amount corresponding to the fluid replacement operation. The amplification process is also performed in accordance with the amount of the reagent added.

In some embodiments, when the sample detector comprises at least a dry chemical detector 51 and a microscopic detector 52, the first dilution device 40 further comprises a first dry chemical dilution device 41 and a first microscopic dilution device 42, and the first dry chemical dilution device 41 corresponds to the dry chemical detector 51, the first microscopic dilution device 42 corresponds to the microscopic detector 52, and the control device 90 is further configured to: it is determined whether to control the first dry chemical dilution apparatus 41 to perform the first dry chemical dilution operation or whether to control the first microscopic dilution apparatus 42 to perform the first microscopic dilution operation, based on the determination result. It can be understood that the solution of the embodiment can be used for not only detecting gynecological leucorrhea samples but also dry chemical detection and microscopic detection.

In other embodiments, as shown in fig. 6 and 7, when the sample detector includes the gynecological conventional detector 50 and the microecological detector 70, the sample detection system 1000 further includes a first dilution device 40 and a second dilution device 60, the first dilution device 40 is disposed corresponding to the gynecological conventional detector 50, the second dilution device 60 is disposed corresponding to the microecological detector 70, and the control device 90 is further configured to: it is determined whether to control the first dilution means 40 to perform the first dilution operation or whether to control the second dilution means 60 to perform the second dilution operation, based on the determination result. Further preferably, when the gynecological routine detector 50 includes the dry chemical detector 51 and the microscopic detector 52, the first dilution device 40 further includes the technical solutions of the first dry chemical dilution device 41 and the first microscopic dilution device 42 described in the previous paragraphs, which are not described herein.

In still other embodiments, as shown in fig. 7, when the sample detector includes a dry chemical detector 51 and a microscopic detector 52, the fluid-filling device 10 further includes a first fluid-filling device and a second fluid-filling device, the first fluid-filling device being disposed corresponding to the dry chemical detector 51 and the second fluid-filling device being disposed corresponding to the microscopic detector 52, and the control device 90 further being configured to: and determining whether to control the first fluid infusion device to execute the first dry chemical dilution operation or whether to control the second fluid infusion device to execute the first microscopic dilution operation according to the judgment result.

In other embodiments, when the sample detector includes the gynecological routine detector 50 and the microecological detector 70, the fluid infusion device 10 further includes a first fluid infusion device and a second fluid infusion device, the first fluid infusion device being disposed corresponding to the gynecological routine detector 50, the second fluid infusion device being disposed corresponding to the microecological detector 70, and the control device 90 further being configured to: and determining whether to control the first fluid infusion device to execute the first dilution operation or whether to control the second fluid infusion device to execute the second dilution operation according to the judgment result.

In the following, a detailed explanation will be given of whether it is determined to perform the dilution operation, as shown in fig. 5A to 8, taking a gynecological leucorrhea sample as an example. Of course, not limited to gynecological specimens, this approach can be used as long as it is necessary to dilute other types of specimens.

The gynecological routine detector 50 is used for performing gynecological routine item detection on a sample fluid to be detected to obtain a first detection result. The gynecological routine item detection comprises, but is not limited to, wet-chip microscopic detection and dry chemical detection for gynecological secretions, wherein the wet-chip microscopic detection item mainly detects formed components (such as mould, hypha, clue cells, trichomonas, epithelial cells, leucocytes, bacillus, cocci, small epithelial cells, erythrocytes and the like) in the gynecological secretions; the dry chemical detection item is mainly to detect the reaction structure of gynecological secretion, and includes Sialidase (SNA), beta-N-acetylglucosaminidase (NAG), leukocyte esterase activity (LE), lactic acid, oxidase, proline aminopeptidase (PIP), PH, hydrogen peroxide, etc., which may also be referred to as sub-categories of the following conventional gynecological detection items. In some embodiments, the dry assay results include at least pH, hydrogen peroxide, leukocyte esterase, lactate, oxidase, sialidase assay, β -glucuronidase, acetaminophycerase, proline aminopeptidase, alkaline phosphatase, clotting enzyme results; the wet lens microscopic examination result at least comprises the cell cleanliness, the white blood cell number, the pus cell number, the red blood cell number and the clue cell detection result.

As shown in fig. 6 to 7, the gynecological conventional detector 50 may be a plurality of instruments or one instrument. For example: as shown in fig. 7, the gynecological routine detector 50 is composed of a dry chemical detector 51 and a microscopic examination detector 52; as shown in fig. 5A and 6, the gynecological conventional detector 50 is formed by combining a dry chemical detector 51 and a microscopic detector 52 in one instrument, i.e., the dry chemical detector 51 and the microscopic detector 52 are only a dry chemical detector and a microscopic detector respectively corresponding to the dry chemical detector and the microscopic detector. In some embodiments, the gynecological routine detector 50 includes a dry chemical detector 51 and/or a microscopic detector 52, where the dry chemical detector 51 is configured to perform dry chemical project detection on a sample liquid to be detected to obtain a dry chemical detection result, and the microscopic detector 52 is configured to perform wet sheet project detection on the sample liquid to be detected to obtain a wet sheet microscopic detection result. The dry chemical detector 51 and the microscopic detector 52 may be separately arranged, i.e. they are separate instruments; the dry chemical detector 51 and the microscopic detection detector 52 can be combined, i.e. both devices are in the same instrument; it may be a single device of the dry chemical detector 51 or a single device of the microscopic detection detector 52; a plurality of dry chemical detectors 51 or a plurality of microscopic detectors 52 can be further arranged, and the forms are changeable and can be adjusted according to the actual demands of users.

The independent equipment or instrument comprises necessary structures such as a sample injection device, a liquid path device, a transfer device and the like. When the dry chemical detector 51 is a device, a reagent detection card and an incubation device are further disposed in the device, the sampling device preferentially distributes the collected sample liquid to be detected onto the reagent detection card, and the sample liquid is transferred to the dry chemical detection module for detection after incubation with the reagent components on the incubation device to obtain a dry chemical detection result. When the microscopic examination detector 52 is a device alone, the necessary structures such as a slide cabin, a sampling device, a transferring device, a microscopic examination device and the like are arranged in the device, and a staining unit is also possible to be arranged, but the microscopic examination detector 52 is not provided with an incubation unit, and the sample liquid to be detected is carried on a slide and is placed under a microscope for microscopic examination. When the dry chemical detector 51 and the microscopic detector 52 are combined into the same device, only a common sampling device, liquid path device and transfer device are needed to be arranged at the moment, and the device also comprises necessary structures such as an incubation device, a microscopic detection device, a dry chemical detection module and the like, and a staining unit can be arranged according to actual needs, but the sample liquid to be detected can be distributed on a single slide and reagent card or a detection card integrated with the slide and the reagent card, and the sample liquid to be detected needs to be incubated before microscopic detection and dry chemical detection of the sample liquid to be detected.

The micro-ecological detector 70 is used for performing micro-ecological project detection on the sample liquid to be detected to obtain a second detection result. In some embodiments, the microecological item detection result (i.e., the second detection result) includes at least: the results of detection of epithelial cells, bacterial population density, lactobacillus ratio, bacterial population diversity, other mixed bacterial ratios, mycoplasma-like corpuscles, chlamydia-like corpuscles, intracellular G-diplococcus, treponema pallidum, blastospores, hyphae, clue cells, trichomonas, leucocytes, erythrocyte, campylobacter alike, gram-negative bacilli, gardnerella/bacteroides, cocci, etc., may also be referred to as the following partial microecological detection item subcategories.

As shown in fig. 5A to 8, after the first sample information detecting device 30 detects the concentration information of the sample liquid to be detected, the control device 90 receives the concentration information of the sample liquid to be detected, and the PD values of the detectors obtained by the transmission method are exemplified as follows. When using the transmission method, the PD value is inversely related to the turbidity, i.e. the higher the PD value, the lower the turbidity, which means that the lower the sample content in the sample liquid to be measured. Upon receiving the PD value, the control device 90 characterizes the concentration information of the sample liquid to be measured with the PD value, and the control device 90 compares the obtained concentration information with the threshold range and determines whether the concentration information satisfies the threshold range. It is noted that the threshold range of the present invention corresponds to the unit of PD value, i.e., the PD value is expressed in turbidity units, the threshold range is also displayed in turbidity units; if the PD value is represented by an electrical signal converted from the light intensity, the threshold range is also represented by a related value of the electrical signal, and the representation manner may be diversified, and the present invention is not limited thereto. While the following examples each represent the PD value and its threshold range in terms of values related to the electrical signal into which the light intensity is converted. Specifically, the PD value is compared with a threshold range preset in advance, and when the control device 90 compares and determines that the concentration information represented by the PD value is too high, after the control device 90 issues a "execute dilution" instruction, the first dilution device 40 executes a first dilution operation and/or the second dilution device 60 executes the second dilution operation, or the first dry chemical dilution device 41 executes a first dry chemical dilution operation and/or the first microscopic dilution device 42 executes a first microscopic dilution operation. Preferably, when gynecological routine detector 50 includes a dry chemical detector 51 and a microscopic detector 52. When the dry chemical detector 51 and the microscopic examination detector 52 may be separate stand-alone apparatuses, the dry chemical detector 51 and the microscopic examination detector 52 are respectively provided with the first dilution device 40, that is, the first dilution device 40 includes the first dry chemical dilution device 41 and the first microscopic examination dilution device 42. Alternatively, when the dry chemical detector 51 and the microscopic detector 52 are integrally provided, that is, when the dry chemical detector 51 and the microscopic detector 52 together form one detector, only one first dilution device 40 is provided. At this time, the control device 90 compares the received PD value with the threshold ranges of different detection items or different subcategories under the same detection item according to the received concentration information of the sample liquid to be detected, and when it is further determined that the concentration is too high and dilution is required, the control device 90 controls the first dilution device 40 to perform the first dilution operation and/or the control device 90 controls the second dilution device 60 to perform the second dilution operation; or the control device 90 controls the first dry chemical dilution device 41, the first microscopic dilution device 42, and the second dilution device 60 to perform the first dry chemical dilution operation, the first microscopic dilution operation, and the second dilution operation, respectively. It will be appreciated that the control device 90 is configured to determine and control the respective diluting devices separately, either synchronously or asynchronously, with the respective timing threads being independent of each other and not interfering.

In some embodiments, when the concentration information satisfies the threshold range, the control device 90 controls the first dilution device 40 and/or the second dilution device 60 to be in an idle state, i.e., not performing the first dilution operation and/or the second dilution operation of the sample liquid to be measured. Preferably, when the gynecological conventional detector 50 is a dry chemical detector 51 and a microscopic detector 52 which are separately provided, that is, the first diluting device 40 includes the first dry chemical diluting device 41 and the first microscopic diluting device 42, when the concentration information satisfies the threshold range, the control device 90 controls the first dry chemical diluting device 41, the first microscopic diluting device 42 and the second diluting device 60 to be in an idle state, that is, does not perform the first dry chemical diluting operation and/or the first microscopic diluting operation and/or the second diluting operation of the sample liquid to be measured.

In other embodiments, as shown in fig. 2, 3 and 4, the control device 90 includes a display unit 91, and when the control device 90 compares and determines that the concentration information represented by the PD value is too low, the control device 90 controls the first diluting device 40 and/or the second diluting device 60 to be in an idle state, i.e. not perform the first diluting operation and/or the second diluting operation of the sample liquid to be measured. At this time, although the first dilution operation and/or the second dilution operation are not performed, the control device 90 may control the gynecological routine detector 50 to perform gynecological routine item detection on the sample liquid to be detected to obtain a first detection result, and/or the control device 90 may control the microecological detector 70 to perform microecological item detection on the sample liquid to be detected to obtain a second detection result, and display the abnormal information on the display unit 91, where the abnormal information may be directly displayed or indirectly displayed on the interface of the display unit 91 by color, character, chinese character, etc., or may further display the cause of the abnormal information on the interface of the display unit 91, for example: the sample concentration is too low, and the abnormality information and the cause thereof may be displayed on the interface of the detection result of the display unit 91 or may be output with a reporting device. Preferably, when the gynecological routine detector 50 is a dry chemical detector 51 and a microscopic chemical detector 52 which are integrally provided, that is, when the concentration of the sample liquid to be measured is too low, the control device 90 controls the only first dilution device 40 to be in an idle state, that is, does not perform the first dilution operation; or when the gynecological routine detector 50 is a dry chemical detector 51 and a microscopic detector 52 which are separately arranged, that is, the first diluting device 40 includes the first dry chemical diluting device 41 and the first microscopic diluting device 42, when the concentration of the sample liquid to be measured is too low, the control device 90 controls the first dry chemical diluting device 41 and the first microscopic diluting device 42 to be in an idle state, that is, does not execute the first dry chemical diluting operation and/or the first microscopic diluting operation of the sample liquid to be measured. Likewise, when the concentration of the sample liquid to be measured is too low, the control device 90 also controls the second diluting device 60 to be in an idle state, i.e., not performing the second diluting operation. At this time, although the first dilution operation is not performed, or although the first dry chemical dilution operation and/or the first microscopic dilution operation are not performed, or although the second dilution operation is not performed, the control device 90 may control the dry chemical detector 51 and the microscopic examination detector 52 and/or the micro-ecological detector 70 to still perform dry chemical item detection, wet sheet microscopic examination item detection and/or micro-ecological item detection on the sample liquid to be detected to obtain the first dry chemical detection result and the first wet sheet microscopic examination result and/or the second detection result, and display these detection results and display abnormality information on the display unit 91, where the abnormality information may also include an abnormality cause.

The sample detection system 1000 is adapted to a plurality of diluting devices by the first sample information detection device 30 and/or the second sample information detection device 80, so that the control device 90 can automatically determine the sample concentration difference of different samples according to the determination result and automatically perform the dilution operation. Therefore, the cost can be saved, the problem of concentration difference of different samples of various clinical projects is solved, the full-automatic clinical diagnosis requirement is met, the unpleasant smell caused by manual operation can be avoided, and the user experience and comfort level are improved.

Because the concentration requirements of the sample liquid to be detected are different in different projects, the threshold range corresponding to the concentration information of the sample liquid to be detected is set to be different according to different detection projects. In some embodiments, the threshold range corresponding to the gynecological routine detection item is different from the threshold range corresponding to the microecological detection item; or the threshold range corresponding to the dry chemical detection item, the threshold range corresponding to the wet sheet microscopic detection item and the threshold range corresponding to the microecological detection item are different, namely the threshold ranges are differently set according to the difference of the detection items and even the difference of subcategories subordinate to the detection items. Preferably, the lowest value and the highest value of the threshold range corresponding to the microecological detection item, the lowest value and the highest value of the threshold range corresponding to the dry chemical detection item and the lowest value and the highest value of the threshold range corresponding to the wet sheet microscopic detection item are increased one by one; or the minimum value and the maximum value of the threshold range corresponding to the microecological detection item, the minimum value and the maximum value of the threshold range corresponding to the dry chemical detection item and the minimum value and the maximum value of the threshold range corresponding to the wet sheet microscopic detection item are reduced one by one. That is, when turbidity detection is performed using the transmission method, the lowest value and the highest value of the above items are increased one by one in order; when turbidity detection is performed using the scattering method, the above items are reduced one by one in the order of their lowest and highest values. This is because the microecological test is a dry plate test, a high concentration sample is required, and thus the minimum and maximum values of the threshold range of PD values (e.g., transmission method) are set lower, preferably lower than those of the dry test item and wet plate microscopy item, in order to meet the requirement; the wet film microscopic examination satisfies that when the formation is observed under a microscope, the minimum value and the maximum value of the corresponding threshold range are both increased without accumulation. Further preferably, the threshold range of the microecological detection item is preferably 10-40, the threshold range of the dry chemical detection item is preferably 20-100, and the threshold range of the wet sheet microscopic detection item is preferably 80-150.

In some embodiments, the threshold range is at least divided into two different gradient threshold ranges according to the sub-category of the detection item, namely a first gradient threshold range and a second gradient threshold range, and the first gradient threshold range and the second gradient threshold range are different, so as to distinguish the gradient threshold ranges of the sub-category of the detection item, and facilitate the identification of the sub-category. This is because the tangible components of wet-sheet microscopy items or subcategories of dry or microecological testing items in a sample are numerous, and a doctor may only address certain subcategories when prescribing patients, for example: mold, trichomonas, etc. Therefore, the pipeline of the invention can also set the gradient threshold range according to different sub-categories (namely the wet film microscopy project, the microecology detection project and the subdivision category under the dry chemical detection project). Preferably, in particular for some particular categories, for example: the infusorium is easily deactivated by the influence of temperature, so that a large amount of dilution is often required to ensure that the ambient temperature is suitable, and when the identification device 100 identifies or the user directly inputs information of a wet film microscopy item or detection of the infusorium sub-item through the input unit 92, the minimum value and the maximum value of the threshold range of the infusorium are increased when a transmission method is used; also for example: the white blood cells have no influence on the temperature, and when the transmission method is used under the wet lens microscopic examination project, the minimum value and the maximum value of the threshold range for detecting the white blood cells are reduced relative to infusions, namely, the maximum value and the minimum value of the first gradient threshold range corresponding to the subcategory infusions are larger than the maximum value and the minimum value of the second gradient threshold range corresponding to the subcategory white blood cells.

In another embodiment, the threshold range is divided into at least a third gradient threshold range and a fourth gradient threshold range according to the same subcategory of different detection items, and the third gradient threshold range and the fourth gradient threshold range are different. For example, the third gradient threshold range is set to be the threshold range of the corresponding trichomonas in the wet film microscopic examination item, and the fourth gradient threshold range is set to be the threshold range of the corresponding trichomonas in the microecological examination item, so that the third gradient threshold range and the fourth gradient threshold range are different. As described above, i.e., when turbidity detection is performed using the transmission method, the lowest value and the highest value of the third gradient threshold range are respectively larger than the lowest value and the highest value of the fourth gradient threshold range; when turbidity detection is performed using the scattering method, the minimum and maximum values of the third gradient threshold range are smaller than the minimum and maximum values of the fourth gradient threshold range, respectively. Of course, not only is the trichomonad, the threshold ranges of other same subcategories in different test items are also different, for example: white blood cells, clue cells, and the like.

In some embodiments, when the concentration information indicates that the concentration of the sample liquid to be measured is too high, and the control device 90 controls the first dilution device 40 to perform the first dilution operation and/or the control device 90 controls the second dilution device 60 to perform the second dilution operation before controlling the first dilution device 40, the control device 90 further selects a preset dilution ratio according to the concentration information, and then controls the first dilution device 40 to perform the first dilution operation and/or controls the second dilution device 60 to perform the second dilution operation according to the selected proper preset dilution ratio. As described above, the preset dilution ratio data is stored in the memory 95 in advance, and the preset dilution ratio may be set to different gradient dilution ratio ranges, that is, there are a plurality of dilution ratio ranges, for example: the first dilution ratio is 2.5-5, the second dilution ratio is 2-2.5, the third dilution ratio is 1.5-2, the fourth dilution ratio is 1-1.5, the fifth dilution ratio is 0.1-1, etc., so that a user can select the proper dilution ratio value on the interface of the display unit 91 according to the displayed concentration information and gradient series range or input the proper dilution ratio value through the input unit 92. The method is more accurate for experienced operators such as senior doctors, and the influence of possible inaccuracy of the subsequent detection result is reduced. In other embodiments, the dilution ratio may be set to different gradient dilution ratio values, i.e., set at a fixed value, and the control device 90 determines and selects an appropriate dilution ratio value based on the concentration information, for example: the first dilution ratio value is 3, the second dilution ratio value is 2.5, the third dilution ratio value is 2, the fourth dilution ratio value is 1.5, the fifth dilution ratio value is 1, and the like, and the fixed dilution ratio value is more beneficial to full-automatic detection without manual operation. Notably, the "dilution ratio" of the present invention refers to: when the sample fluid sucked from the sample container by the in-instrument sampling device needs to be diluted, the dilution device needs to add a "numerical value or a range of multiples" of the amount of the sucked sample fluid, for example, based on the amount of the sample fluid sucked from the sample container: the amount of the sample liquid to be detected sucked into the gynecological routine detector 50 by the sampling device is 100ul, and if the proper dilution ratio selected by the control device 90 or the dilution ratio value selected/input by the user is 2.5, the dilution device needs to add 250ul of dilution liquid; however, when only partial dilution is required for the sample fluid drawn from the sample container by the in-instrument sampling device, the dilution device needs to add a "multiple value or multiple range" of the difference between the total amount of the sample fluid drawn from the sample container and the amount of the sample fluid not to be diluted, for example: the amount of the sample liquid to be detected sucked into the gynecological routine detector 50 by the sampling device is 100ul, if the sample liquid to be detected is judged to be 30ul for the dry chemical detection item which does not need to be diluted but for the wet sheet microscopy item which needs to be diluted, 70ul still remains after the transfer, and if the proper dilution ratio selected by the control device 90 or the dilution ratio value selected/input by the user is 2.5, the dilution device needs to be added with 175ul of dilution liquid.

In some embodiments, the dilution ratio range or dilution ratio value described above is associated with a threshold range corresponding to the concentration information. Preferably, the threshold range stored in the memory 95 of the control device 90 and the preset dilution ratio are mapped, and may be one-to-one, many-to-many, etc. For example, after the first sample information detecting device 30 detects the PD value of the sample liquid to be tested, the control device 90 receives the PD value, compares the PD value with the threshold range, if it is determined that the concentration is too high, selects an appropriate preset threshold range, maps the appropriate preset threshold range to an appropriate preset dilution ratio according to the appropriate threshold range, and after the control device 90 issues an instruction of "execute dilution", the first dilution device 40 executes the first dilution operation and/or the second dilution device 60 executes the second dilution operation.

Preferably, when gynecological routine detector 50 includes a dry chemical detector 51 and a microscopic detector 52. When the dry chemical detector 51 and the microscopic examination detector 52 may be separate stand-alone apparatuses, the dry chemical detector 51 and the microscopic examination detector 52 are respectively provided with the first dilution device 40, that is, the first dilution device 40 includes the first dry chemical dilution device 41 and the first microscopic examination dilution device 42. Alternatively, when the dry chemical detector 51 and the microscopic detector 52 are integrally provided, that is, when the dry chemical detector 51 and the microscopic detector 52 together form one detector, only one first dilution device 40 is provided. At this time, the control device 90 receives the PD value, compares the PD value with the threshold ranges of different test items or different sub-categories under the same test item, and when it is determined that the concentration is too high and dilution is required, selects the threshold range suitable for the item or the sub-category item, maps the threshold range to a suitable dilution ratio, and the control device 90 controls the first dilution device 40 to perform the first dilution operation and/or the control device 90 controls the second dilution device 60 to perform the second dilution operation. At this time, the control device 90 receives the PD value, compares the PD value with threshold ranges of different test items or different sub-categories under the same test item, and when it is determined that the concentration is too high and dilution is required, selects a threshold range suitable for the item or the sub-category item, maps the threshold range to a suitable dilution ratio, and the control device 90 controls the first dry chemical dilution device 41 and/or the first microscopic dilution device 42 and/or the second dilution device 60 to perform the first dry chemical dilution operation and/or the first microscopic dilution operation and/or the second dilution operation, respectively. At this time, although the same PD value, the control device 90 may perform different operations due to different threshold ranges and dilution ratios corresponding to different items or different sub-category items, for example: when the first sample information detecting device 30 detects that the PD value of the sample to be detected is 50 by transmission method, since the threshold range of the microecological detection item preset in the memory 95 is preferably 10-40, the threshold range of the dry chemical detection item is preferably 20-100, and the threshold range of the wet film microscopic detection item is preferably 80-150, the control device 90 determines that the concentration information represented by the PD value is too low for the microecological item, and the concentration is too high for the dry chemical detection item when the threshold range is satisfied, so the control device 90 sends an instruction of "execute aggregation" for the microecological detection item, sends an instruction of "execute dilution" for the wet film microscopic detection item, and does not need dilution for the dry chemical detection item, but leaves the first dry chemical diluting device 41 and the second diluting device 60 in idle state. Before the first microscopic dilution device 42 performs dilution, the control device 90 selects a suitable dilution ratio corresponding to the threshold range, and then controls the first microscopic dilution device 42 to absorb the dilution liquid corresponding to the dilution ratio according to the dilution ratio so as to dilute the sample to be tested. It will be appreciated that the control device 90 is configured to determine and control the respective diluting devices separately, either synchronously or asynchronously, with the respective timing threads being independent of each other and not interfering.

After the control device 90 controls a plurality of or a certain dilution device to perform the dilution operation, the gynecological routine detector 50 and/or the microecological detector 70 detects the sample to be detected, and obtains a first detection result and/or a second detection result. At this time, the control device 90 performs amplification processing on the first detection result and/or the second detection result according to the above-selected appropriate preset dilution ratio. As shown in fig. 3 and 4, the amplification processing step may be performed together with the detection result, or the actual detection result may be output and then subjected to the result amplification processing, and these steps may be automatically output by the control device 90, or the appropriate dilution ratio and the actual detection result may be displayed on the interface of the display unit 91, and the user may perform the amplification processing on the display interface by himself.

The parameters used in the amplification process may have preset gradient values or gradient ranges set in advance in the memory 95, and the dilution ratio ranges or dilution ratio values are associated with the amplification process parameters, and the control device 90 performs the amplification process on the first detection result and/or the second detection result according to the amplification process parameters. Preferably, the preset dilution ratio and the amplification processing parameter are mapped, and may be one-to-one, many-to-many, etc. After the control device 90 manually selects or automatically maps to the appropriate preset dilution ratio on the display interface according to the appropriate threshold range, the control device 90 may also manually select or automatically map to the appropriate amplification process parameter on the display interface according to the appropriate preset dilution ratio. The amplification process parameters may be set to different gradient amplification process parameter ranges, i.e. there are multiple amplification process parameter ranges, for example: the first amplification parameter range is 2.5-5, the second amplification parameter range is 2-2.5, the third amplification parameter range is 1.5-2, the fourth amplification parameter range is 1-1.5, the fifth amplification parameter range is 0.1-1, etc., so that the user can select the appropriate amplification parameter on the display interface of the display unit 91 according to the displayed dilution ratio gradient range or the dilution ratio value, or input the appropriate amplification parameter through the input unit 92, for example: after the detection result is obtained, proper amplification processing parameters are selected to carry out amplification processing, so that the method is more accurate for operators such as experienced senior doctors, and the influence of possible inaccuracy of the subsequent detection result is reduced. In other embodiments, the amplification parameters may be set to different gradient amplification values, i.e., at fixed values, and the control device 90 determines and selects the appropriate amplification value based on the dilution ratio range or value, for example: the first amplification processing parameter value is 3, the second amplification processing parameter value is 2.5, the third amplification processing parameter value is 2, the fourth amplification processing parameter value is 1.5, the fifth amplification processing parameter value is 1, and the like, and the fixed amplification processing parameter value is more beneficial to full-automatic detection without manual operation. Notably, the "amplification processing parameter" of the present invention means: the detection result is amplified by a certain "multiple value or multiple range", for example: when 8 (infusions) are displayed in the infusions detection result, and the control device controls the items to be diluted, the control device 90 will amplify the detection result by a factor of 2.5 according to the selected suitable amplification processing parameter or the amplification processing parameter selected/input by the user to be 2.5, and the detection result finally output is 20 infusions. Of course, not limited to trichomonas, for example: clue cells, white blood cells, red blood cells, etc. are within the scope of the present invention.

Preferably, after the first sample information detecting device 30 detects the PD value of the sample liquid to be detected, the control device 90 receives the PD value, compares the PD value with the threshold range, if it is determined that the concentration is too high, selects an appropriate preset threshold range, maps the appropriate preset threshold range to an appropriate preset dilution ratio according to the appropriate threshold range, and after the control device 90 issues an instruction of "execute dilution", the first diluting device 40 executes the first diluting operation and/or the second diluting device 60 executes the second diluting operation. The rear control device 90 controls the gynecological routine detector 50 to perform gynecological routine item detection to obtain a first detection result, and/or the control device 90 controls the microecological detector 70 to perform microecological item detection to obtain a second detection result, the control device 90 automatically maps out a suitable amplification parameter according to a suitable preset dilution ratio or selects or inputs a suitable value of the amplification parameter, and the rear control device 90 performs amplification processing on the first detection result and/or the second detection result according to the amplification parameter. The amplification processing steps of the first detection result and/or the second detection result are not related, but are determined according to whether the first detection result and/or the second detection result have a dilution step or not. More specifically, whether to perform the amplification process and the appropriate amplification process parameters may be determined according to whether or not the appropriate dilution ratio is selected.

Preferably, the gynecological routine detector 50 includes a dry chemical detector 51 and a microscopic detector 52. When the dry chemical detector 51 and the microscopic examination detector 52 may be separate stand-alone apparatuses, the dry chemical detector 51 and the microscopic examination detector 52 are respectively provided with the first dilution device 40, that is, the first dilution device 40 includes the first dry chemical dilution device 41 and the first microscopic examination dilution device 42. Alternatively, when the dry chemical detector 51 and the microscopic detector 52 are integrally provided, that is, when the dry chemical detector 51 and the microscopic detector 52 together form one detector, only one first dilution device 40 is provided. At this time, the control device 90 receives the PD value, compares the PD value with the threshold ranges of different test items or different sub-categories under the same test item, when it is determined that the concentration is too high and needs to be diluted, then selects the threshold range suitable for the item or the sub-category item, maps the threshold range to a suitable dilution ratio, and then controls the first dilution device 40 to perform the first dilution operation and/or controls the second dilution device 60 to perform the second dilution operation, or controls the first dry chemical dilution device 41 to perform the first dry chemical dilution operation and/or controls the first microscopic dilution device 42 to perform the first microscopic dilution operation and/or controls the second dilution device 60 to perform the second dilution operation, and then controls the dry chemical detector 51 to perform the dry chemical item detection to obtain the dry chemical detection result and/or controls the microscopic detector 52 to perform the wet sheet item detection to obtain the wet sheet microscopic detection result and/or controls the micro-item detection to obtain the second detection result, or controls the control device 90 to perform the micro-item detection to perform the second dilution operation, and then controls the device 90 to perform the appropriate amplification processing according to the preset amplification parameters and/or the suitable amplification parameters. It will be appreciated that, although the first sample information detecting device 30 detects the PD value of the same sample to be detected, because the threshold range, the dilution ratio, and the amplification process parameter corresponding to the different items or the different sub-category items corresponding to the same sample liquid to be detected are not completely the same, the control device 90 performs different operations according to the same PD value, for example: when the first sample information detection device 30 detects that the PD value of the sample to be detected is 50 by using the transmission method, the control device 90 determines that the concentration information represented by the PD value is too low for the microecological item, because the threshold range of the microecological detection item preset in the memory 95 is preferably 10-40, the threshold range of the dry chemical detection item is preferably 20-100, and the threshold range of the wet film microscopic detection item is preferably 80-150; for the dry chemical detection item, the threshold range is met, a proper dilution ratio is not required to be selected, and the detection result is not required to be amplified; however, for wet-film microscopy, too high a concentration requires selection of a suitable dilution ratio, and amplification of the detection result. Therefore, the control device 90 issues an instruction of "execute aggregation" for the microecological inspection item, issues an instruction of "execute dilution" for the wet film microscopic inspection item, and issues an instruction of "idle waiting" for the dry chemical inspection item, i.e., without dilution, to make it in an idle state. Before the first microscopic dilution device 42 performs dilution, the control device 90 first selects a suitable dilution ratio corresponding to the threshold range, then controls the first microscopic dilution device 42 to absorb the amount of the dilution liquid corresponding to the dilution ratio according to the dilution ratio, performs wet-film microscopic examination after dilution to obtain a wet-film microscopic examination result, and amplifies the wet-film microscopic examination result, so that the obtained dry chemical examination result and the obtained micro-ecological examination result do not need to select suitable amplification parameters and amplify the examination result. It will be appreciated that the control device 90 is configured to determine and control the respective diluting device and detecting device, and may or may not be synchronized, and the respective timing threads are independent of each other and do not interfere with each other.

Preferably, when the dry chemical detector 51 and the microscopic detector 52 are integrally provided, that is, when the dry chemical detector 51 and the microscopic detector 52 together form one detector, only one first dilution device 40 is provided. If the control device 90 determines that the PD value represents the concentration information that is too high for one of the dry chemical detection item and the wet lens microscopy item and is normal, although there is only one first dilution device 40, after obtaining the PD value represents the concentration information and performing the determination comparison, the transfer device may first transfer the appropriate sample liquid to be tested into the incubation device or the detection device for incubation reaction or directly performing detection, and at this time, the sample liquid to be tested is not diluted. And then diluting the rest sample liquid to be detected according to the dilution ratio mapped in the corresponding threshold range or the dilution ratio selected by the user, and transferring the diluted sample liquid to an incubation device or a detection device by a transfer device for incubation reaction or direct detection. Thus, even if there is only one dilution device, the control device 90 can also perform the dilution operation independently for two different detection devices.

In summary, the sample detection system 1000 provided by the application can select different suitable reagent amounts according to the actual demands of users by matching different detection samples, thereby saving the cost of sample detection, meeting the demand of automatic fluid replenishment and improving the detection accuracy and detection efficiency. And this sample detecting system 1000 system detects the concentration of different samples through setting up sample information detection device and a plurality of diluting device to whether carry out different dilution operation to the automation of a plurality of different detection projects is confirmed, and the automation has eliminated the individual of patient and has had the difference or the difference influence of the collection sample volume that doctor manual operation's deviation leads to, can improve the detection efficiency to the sample, and degree of automation is high, and the user comfort that reinforcing gynaecology sample detected.

Referring to fig. 9 to 10, the present application further provides a sample detection method, which can be applied to the sample detection system 1000, and the sample detection method includes the following steps:

s01, responding to an input operation instruction of the reagent related information, and acquiring the reagent related information corresponding to the input operation instruction;

s02, acquiring sample identity information of a biological sample on the attachment and item association information of a target sample corresponding to the sample identity information;

s03, comparing the reagent related information with the project related information, and performing elution operation on the target sample to form a sample liquid to be detected; the eluting liquid amount corresponding to the eluting operation is a corresponding value of the small value in the reagent association information and the item association;

s04, executing detection corresponding to the project association information on the sample liquid to be detected.

In some embodiments, as shown in fig. 10, after the step S03 and before the step S04, the method further includes:

s031: when the small value is the reagent association information, determining to carry out fluid replacement operation on the sample fluid to be tested; when the small value is the project related information or the reagent related information is the same as the project related information, determining that the liquid supplementing operation is not performed on the sample liquid to be tested.

In some embodiments, the reagent addition corresponding to the fluid replacement operation is a difference corresponding value of the reagent-related information and the item-related information.

In some embodiments, the reagent-related information is selected from any one of a preset reagent amount, preset item information, preset identification information.

s032: performing concentration information detection operation on the sample liquid to be detected;

s033: and judging whether the concentration information meets a threshold range, and determining whether to execute the dilution operation according to a judging result.

s034: when the liquid supplementing operation and the diluting operation are determined to be performed, performing one-time liquid adding operation on the sample liquid to be tested, wherein the reagent adding amount corresponding to the one-time liquid adding operation is the sum of the diluting amount corresponding to the diluting operation and the liquid supplementing amount corresponding to the liquid supplementing operation; or when the fluid supplementing operation and the dilution operation are determined to be performed, the fluid supplementing operation and the dilution operation are respectively performed on the sample fluid to be tested.

In some embodiments, as shown in fig. 10, the step S04 further includes:

s05: when the liquid supplementing operation is determined to be executed, amplifying the detection result according to the reagent adding amount corresponding to the liquid supplementing operation; or when the dilution operation is determined to be executed, amplifying the detection result according to the reagent addition amount corresponding to the dilution operation; or when the liquid supplementing operation and the diluting operation are determined to be executed, amplifying the detection result according to the sum of the diluting amount corresponding to the diluting operation and the liquid supplementing amount corresponding to the liquid supplementing operation.

In some embodiments, the biological sample on the attachment is placed in a sample container, and in particular, the target sample is applied to a sampling tool (e.g., a flocked swab, a regular swab, etc.).

In some embodiments, in step S03, the item association information includes item information to be inspected, or the item association information includes item information to be inspected and a target reagent amount corresponding based on the item information to be inspected. The item information to be inspected comprises at least one of a microecological item, a dry chemical item and a microscopic inspection item.

In some embodiments, when the fluid replacement operation is determined to be performed on the sample fluid to be tested, the amount of the collected sample to be tested is reduced when the gynecological routine item detection is performed on the sample fluid to be tested, and the amount of the collected sample to be tested is not reduced when the microecological item detection is performed on the sample fluid to be tested. The method is characterized in that the microecological project detection is high in concentration of the sample liquid to be detected, and even the sampling tool covered with the sample liquid to be detected can be directly used for dry smear on a glass slide during manual operation, so that in order to ensure that the microecological detection project has a sample with high enough concentration, the sample liquid quantity of the microecological detection project is preferably not subjected to liquid supplementing operation, but the sample liquid quantity to be detected, which is required to be collected, is reduced in the gynecological conventional project, and then the sample liquid to be detected is subjected to dilution operation to carry out liquid supplementing operation and/or dilution operation so as to improve detection accuracy.

In some embodiments, when it is determined that the fluid replacement operation is performed on the sample fluid to be measured, and it is determined that the concentration information indicates that the concentration of the sample fluid to be measured is too high, the dilution operation of the gynecological routine item is performed. It will be appreciated that the dilution liquid amount in the gynecological routine program dilution operation is the sum of the compensation liquid amount and the dilution liquid amount added according to the dilution ratio. The gynecological conventional project diluting device distributes the total amount of dilution.

In another embodiment, when it is determined to perform the fluid replacement operation on the sample fluid to be tested, a first dilution operation is performed to replace the fluid; and when the concentration information is judged to represent that the concentration of the sample liquid to be measured is too high, performing gynecological routine project dilution operation to reduce the concentration of the sample. It can be understood that the dilution liquid amount of the gynecological routine program dilution operation preferably executed at this time is only the complement liquid amount, and the gynecological routine program dilution operation executed later is the liquid amount for diluting the sample to be measured.

In some embodiments, the first test result is amplified according to the amount of fluid that is replenished. Preferably, the reagent addition amount and the amplification factor corresponding to the fluid infusion operation are positively correlated, that is, the larger the reagent addition amount is, the larger the amplification factor is. Of course, whether the instrument is automated or manually operated, the range or value of the magnification can be flexibly selected.

In some embodiments, the gynecological routine program includes a dry chemical program and a wet sheet program, and the sample detection method includes the steps of:

judging whether the concentration information meets a threshold range, and determining whether to execute a first dry chemical dilution operation and/or a first microscopic dilution operation and/or a second dilution operation according to a judging result;

performing dry chemical project detection on the sample liquid to be detected to obtain a first dry chemical detection result;

carrying out wet sheet project detection on a sample liquid to be detected to obtain a first wet sheet microscopic detection result;

and carrying out microecological project detection on the sample liquid to be detected to obtain a second detection result.

Preferably, after the fluid infusion operation and/or the dilution operation are performed on the corresponding detection items as described above, amplification processing is required for the corresponding detection results. The amplification treatment parameters and the corresponding dilution ratio and the corresponding reagent addition amount of the fluid infusion operation are positively correlated.

It should be noted that the above only uses gynecological leucorrhea sample as an example, and the application is not limited to this type of sample. Moreover, the specific explanation and the supplementation of the sample detection method of the present application may refer to the explanation of the sample detection system 1000, and will not be repeated herein.

In summary, the above-mentioned scheme has solved present sample check out test set can not match the demand that different sample detected items should select different eluent amounts, has saved reagent cost, has especially solved the eluent amount of single setting at present and probably brought can not support the sample to be measured to finish the detection of all items, even consequently can lead to the instrument in-process of detecting alert even harm instrument's problem. The sample detection system 1000 provided by the application can match different detection samples according to the actual demands of users to select different matched reagent amounts, and has high degree of automation and enhances the user experience. In addition, the detection method also solves the problem that the sample concentration cannot be adjusted in time in the prior art, and can determine whether different liquid supplementing operation schemes are executed according to different detection projects, so that the operation efficiency and the detection accuracy are improved, the defects of the current commercial products are overcome, the use of the eluent amount is reduced, the sample detection cost is saved, and the market demand for sample detection can be met.

Embodiments of the present invention further provide a storage medium, i.e., a computer-readable storage medium, in which an executable program is stored, which when executed by the processor 94, implements the use of the control device 90 included in the sample detection system 1000 or implements the steps of the sample detection system method.

In some embodiments, the processor 94 may be a central processing unit (Central Processing Unit, CPU), which may also be other general purpose processors, digital signal processors (Digital Signal Processor, DSPs), application specific integrated circuits (Application Specific Integrated Circuit, ASICs), field programmable gate arrays (Field-Programmable Gate Array, FPGAs) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or the like. And the memory 95 stores various computer programs such as an operating system and application programs for execution by the processor 94 and data required for execution of the computer programs. In the process of detecting and analyzing the sample to be detected, if necessary, the data stored locally can be stored in the memory 95.

It will be appreciated that there is no necessarily order between the steps described above, for example: the step S03 may be performed before the step S02, or the step S02 may be performed before the step S03. Of course, the order of all operations or steps/sub-steps of the present invention is not limited to the examples, and some steps may be performed in parallel or even in a sequential order, which falls within the technical scope of the present invention. For example: the operation of judging whether the density information satisfies the threshold range or judging whether the liquid amount is sufficient is not necessarily in the order of tandem, and the subsequent operation caused thereby is also not in the absolute order of tandem. The following examples and serial numbers are for illustration only, and the order of the steps can be flexibly adjusted, which falls within the scope of the present invention.

The description of the medium embodiments above is similar to that of the method embodiments above, with similar advantageous effects as the method embodiments. For technical details not disclosed in the storage medium embodiments of the present invention, please refer to the description of the method embodiments of the present invention for understanding.

In the embodiment of the present invention, if the above-mentioned sample detection method is implemented in the form of a software functional module and sold or used as a separate product, it may also be stored in a computer readable storage medium. Based on such understanding, the technical solutions of the embodiments of the present invention may be embodied in essence or a part contributing to the prior art in the form of a software product stored in a storage medium, including several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute all or part of the methods described in the embodiments of the present invention. And the aforementioned storage medium includes: various media capable of storing program codes, such as a usb disk, a removable hard disk, a Read Only Memory (ROM), a magnetic disk, or an optical disk. Thus, embodiments of the invention are not limited to any specific combination of hardware and software.

It is to be understood that the terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this specification and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

It should also be understood that the term "and/or" as used in the present specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.

The foregoing embodiment numbers of the present application are merely for the purpose of description, and do not represent the advantages or disadvantages of the embodiments. While the application has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes and substitutions of equivalents may be made and equivalents will be apparent to those skilled in the art without departing from the scope of the application. Therefore, the protection scope of the application is subject to the protection scope of the claims.

Claims

1. The sample detection system is characterized by comprising an elution device, a fluid infusion device, a first sample information detection device, a sample detector and a control device, wherein the control device is connected with the elution device, the fluid infusion device, the first sample information detection device and at least one sample detector;

the control device is used for:

Controlling the first sample information detection device to execute concentration information detection operation on the sample liquid to be detected;

receiving concentration information of the sample liquid to be detected;

determining whether to execute a dilution operation according to the judgment result;

controlling the sample detector to perform detection corresponding to the item association information on the sample liquid to be detected;

wherein the control device is further configured to: determining whether to control the liquid supplementing device to supplement the liquid to the sample liquid to be tested, and when the small value is the reagent related information, determining to control the liquid supplementing device to supplement the liquid to the sample liquid to be tested; and controlling the fluid replacement device to perform the fluid replacement operation and the dilution operation when it is determined to perform the dilution operation and the fluid replacement operation.

2. The sample testing system of claim 1, further comprising a fluid replacement device, said control device being coupled to said fluid replacement device, said control device further configured to:

and when the small value is the item association information or the reagent association information is the same as the item association information, determining to control the fluid infusion device to not conduct fluid infusion operation on the sample fluid to be tested.

3. The sample detection system according to claim 2, wherein the reagent addition amount corresponding to the fluid replacement operation is a corresponding value of a difference between the reagent-related information and the item-related information.

4. The sample detection system according to claim 1, wherein the reagent-related information is selected from any one of a preset reagent amount, preset item information, preset identification information; and/or the number of the groups of groups,

the item association information comprises to-be-detected item information, or the item association information comprises to-be-detected item information and a target reagent amount corresponding to the to-be-detected item information.

5. The sample detection system according to claim 4, wherein the reagent-related information is the preset reagent amount, and the item-related information is item information to be detected and the target reagent amount corresponding based on the item information to be detected; wherein the comparing the reagent-related information with the item-related information, the controlling the eluting device to perform an eluting operation on the target sample to form a sample liquid to be measured includes:

comparing the preset reagent amount with the target reagent amount to obtain a reagent small value;

controlling the elution device to perform an elution operation on the target sample so as to form the sample liquid to be detected; wherein the amount of the eluent corresponding to the elution operation is the small value of the reagent.

6. The sample detection system of claim 4, wherein the reagent-related information is the preset item information, and the item-related information is the item information to be detected; wherein the comparing the reagent-related information with the item-related information, the controlling the eluting device to perform an eluting operation on the target sample to form a sample liquid to be measured includes:

comparing the preset project information with the project information to be detected to obtain a project small value;

acquiring a corresponding reagent small value based on the item small value;

7. The sample detection system according to claim 4, wherein the reagent-related information is the preset identification information, and the item-related information is the item-to-be-detected information; wherein the comparing the reagent-related information with the item-related information, the controlling the eluting device to perform an eluting operation on the target sample to form a sample liquid to be measured includes:

acquiring the preset reagent amount based on the preset identification information;

Acquiring the target reagent amount based on the item information to be detected;

controlling the elution device to perform an elution operation on the target sample so as to form the sample liquid to be detected; wherein the amount of the eluent corresponding to the elution operation is the small value of the reagent;

or alternatively, the process may be performed,

the comparing the reagent-related information with the item-related information, and controlling the eluting device to perform an eluting operation on the target sample to form a sample liquid to be measured includes:

acquiring the preset project information based on the preset identification information;

acquiring a corresponding reagent small value based on the item small value;

controlling the elution device to perform elution operation on the target sample so as to form a sample liquid to be detected; wherein the amount of the eluent corresponding to the elution operation is the small value of the reagent.

8. The sample detection system according to claim 4, wherein the reagent-related information is the preset identification information, and the item-related information is the item information to be detected and the target reagent amount corresponding based on the item information to be detected; wherein the comparing the reagent-related information with the item-related information, the controlling the eluting device to perform an eluting operation on the target sample to form a sample liquid to be measured includes:

or alternatively, the process may be performed,

acquiring a corresponding reagent small value based on the item small value;

9. The sample detection system according to claim 1, wherein the determination as to whether the concentration information satisfies a threshold range is performed to obtain a determination result; determining whether to perform the diluting operation according to the judging result includes:

When the concentration of the sample liquid to be measured, which is represented by the concentration information, is judged to be too high, determining to execute dilution operation;

obtaining the dilution quantity corresponding to the dilution operation;

the dilution operation is performed based on the dilution amount.

10. The sample detection system according to claim 1, wherein when the control device determines that the dilution operation and the fluid replacement operation are performed, a reagent addition amount of the fluid replacement device is a sum of a dilution amount corresponding to the dilution operation and a fluid replacement amount corresponding to the fluid replacement operation.

11. The sample detection system of claim 1, further comprising an aggregation device, wherein the sample detector comprises a micro-ecological detector, wherein the control device is coupled to the aggregation device and the micro-ecological detector, and wherein the control device is further configured to: and when the concentration of the sample liquid to be detected, which is represented by the concentration information, is judged to be too low, controlling the aggregation device to execute aggregation operation on the sample liquid to be detected transferred to the microecological detector.

12. The sample detection system of claim 9, further comprising a first dilution device, the control device being coupled to the first dilution device, the control device further configured to: when determining to execute the dilution operation, acquiring the dilution amount corresponding to the dilution operation; controlling the first dilution means to perform the dilution operation based on the dilution amount;

And/or the number of the groups of groups,

the control device is also used for: and amplifying a detection result obtained by executing the detection corresponding to the item association information according to the dilution amount corresponding to the dilution operation.

13. The sample testing system of claim 1, further comprising a fluid replacement device, said control device being coupled to said fluid replacement device, said control device further configured to determine whether to control said fluid replacement device to perform a fluid replacement operation on said sample fluid to be tested, said sample testing device comprising a dry chemical testing device and a microscopic testing device, said control device further configured to:

when the liquid supplementing device is determined to be controlled to conduct the liquid supplementing operation on the sample liquid to be tested, conducting dry chemical liquid supplementing operation on the sample liquid to be tested transferred to the dry chemical detector and conducting microscopic liquid supplementing operation on the sample liquid to be tested transferred to the microscopic detector.

14. The sample detection system of claim 13, wherein the dry chemical fluid replacement operation corresponds to a less fluid replacement than the microscopic fluid replacement operation.

15. The sample testing system of claim 1, further comprising a fluid infusion device, said control device being coupled to said fluid infusion device, said control device further configured to determine whether to control said fluid infusion device to infuse said sample fluid to be tested, said sample testing device comprising a gynecological routine testing device and a microecological testing device, said control device further configured to: when the fluid replacement device is determined to be controlled to perform the fluid replacement operation on the sample fluid to be tested, performing the fluid replacement operation on the sample fluid to be tested transferred to the gynecological conventional detector and/or not performing the fluid replacement operation on the sample fluid to be tested transferred to the microecological detector;

And/or the number of the groups of groups,

the sample detection system further comprises a liquid supplementing device, the control device is connected with the liquid supplementing device, the control device is further used for determining whether to control the liquid supplementing device to supplement the sample liquid to be detected, and the control device is further used for: controlling the sample detector to perform detection corresponding to the item association information on the sample liquid to be detected to obtain a detection result; when the fluid replacement operation is determined to be executed, amplifying a detection result obtained by the detection corresponding to the project related information according to the reagent addition amount corresponding to the fluid replacement operation;

and/or the number of the groups of groups,

the biological sample is selected from any one of sputum, nasopharyngeal secretion, cervical secretion and vaginal secretion;

and/or the number of the groups of groups,

the sample detection system further comprises a display unit, the control device is connected with the display unit, and the control device is further used for: based on the reagent related information displayed by the display unit, responding to an input operation instruction of the reagent related information, and acquiring a preset reagent amount corresponding to the input operation instruction;

and/or the number of the groups of groups,

the sample detection system further comprises an identification device, the control device is connected with the identification device, and the control device is further used for: controlling the identification device to acquire sample identity information of a biological sample on the attachment and item association information of the target sample corresponding to the sample identity information;

And/or the number of the groups of groups,

the sample detection system further comprises an output unit, and the control device is further configured to: and if the detection corresponding to the item association information is executed and a detection result is obtained, responding to the operation instruction, and controlling the output unit to output the detection result of the target sample.

16. The sample detection system of claim 12, wherein the first dilution device further comprises a first dry chemical dilution device and a first microscopic dilution device, the sample detector comprising a dry chemical detector and a microscopic detector, the first dry chemical dilution device corresponding to the dry chemical detector, the first microscopic dilution device corresponding to the microscopic detector, the control device further configured to: determining whether to control the first dry chemical dilution device to execute a first dry chemical dilution operation or whether to control the first microscopic dilution device to execute a first microscopic dilution operation according to the judgment result; or alternatively, the process may be performed,

the sample detection system further comprises a first dilution device and a second dilution device, the sample detector comprises a gynecological routine detector and a microecological detector, the first dilution device corresponds to the gynecological routine detector, the second dilution device corresponds to the microecological detector, and the control device is further used for: determining whether to control the first dilution device to execute a first dilution operation or whether to control the second dilution device to execute a second dilution operation according to the judgment result; or alternatively, the process may be performed,

The sample detecting system further comprises a liquid supplementing device, the control device is connected with the liquid supplementing device, the control device is further used for determining whether to control the liquid supplementing device to supplement liquid to the sample liquid to be detected, the liquid supplementing device further comprises a first liquid supplementing device and a second liquid supplementing device, the sample detecting device comprises a dry chemical detecting instrument and a microscopic detecting instrument, the first liquid supplementing device corresponds to the dry chemical detecting instrument, the second liquid supplementing device corresponds to the microscopic detecting instrument, and the control device is further used for: determining whether to control the first fluid infusion device to execute a first dry chemical dilution operation or whether to control the second fluid infusion device to execute a first microscopic dilution operation according to the judging result; or alternatively, the process may be performed,

the sample detecting system further comprises a liquid supplementing device, the control device is connected with the liquid supplementing device, the control device is further used for determining whether to control the liquid supplementing device to supplement liquid to the sample liquid to be detected, the liquid supplementing device further comprises a first liquid supplementing device and a second liquid supplementing device, the sample detecting device comprises a gynecological conventional detector and a microecological detector, the first liquid supplementing device corresponds to the gynecological conventional detector, the second liquid supplementing device corresponds to the microecological detector, and the control device is further used for: and determining whether to control the first fluid infusion device to execute a first dry dilution operation or whether to control the second fluid infusion device to execute a second dilution operation according to the judging result.

17. A sample detection method applied to the sample detection system according to any one of claims 1 to 16, wherein the sample detection method comprises:

performing concentration information detection operation on the sample liquid to be detected;

judging whether the concentration information meets a threshold range or not, and determining whether to execute dilution operation or not according to a judging result;

when the small value is the reagent related information and the fluid replacement operation and the dilution operation are determined to be performed, performing the fluid replacement operation and/or the dilution operation on the sample fluid to be tested;

18. The sample detection method according to claim 17, wherein after comparing the reagent-related information and the item-related information and performing an elution operation on the target sample to form a sample liquid to be detected, further comprising:

and when the small value is the item association information or the reagent association information is the same as the item association information, determining that the liquid supplementing operation is not performed on the sample liquid to be tested.

19. The method according to claim 18, wherein the reagent addition amount corresponding to the fluid replacement operation is a difference value corresponding to the reagent-related information and the item-related information; and/or the number of the groups of groups,

the reagent association information is selected from any one of preset reagent amount, preset project information and preset identification information; and/or the number of the groups of groups,

20. The sample detection method according to claim 18, characterized by further comprising, after the performing of the elution operation on the target sample to form a sample liquid to be detected, and before the performing of the detection step corresponding to the item association information on the sample liquid to be detected:

When the fluid supplementing operation and the dilution operation are determined to be performed, performing one fluid adding operation on the sample fluid to be tested, wherein the addition amount of the reagent corresponding to the one fluid adding operation is the sum of the dilution amount corresponding to the dilution operation and the fluid supplementing amount corresponding to the fluid supplementing operation; or when the fluid replacement operation and the dilution operation are determined to be performed, performing the fluid replacement operation and the dilution operation on the sample fluid to be tested respectively;

or alternatively, the process may be performed,

after executing the detection step corresponding to the item association information on the sample liquid to be detected, the method further comprises:

when the fluid replacement operation is determined to be executed, amplifying a detection result obtained by detecting the item association information according to the reagent addition amount corresponding to the fluid replacement operation; or when the dilution operation is determined to be executed, amplifying a detection result obtained by the detection of the item association information according to the reagent addition amount corresponding to the dilution operation; or when the fluid replacement operation and the dilution operation are determined to be executed, amplifying the detection result obtained by executing the detection of the item association information according to the sum of the dilution amount corresponding to the dilution operation and the fluid replacement amount corresponding to the fluid replacement operation.

21. A storage medium having stored therein an executable program which when executed performs the use of the control device comprised by the sample detection system of any one of claims 1 to 16, or which when executed performs the steps of the sample detection method of any one of claims 17 to 20.