CN116536132A - Test method and reaction device for degrading silicone rubber by co-metabolism flora - Google Patents
Test method and reaction device for degrading silicone rubber by co-metabolism flora Download PDFInfo
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- CN116536132A CN116536132A CN202310303900.2A CN202310303900A CN116536132A CN 116536132 A CN116536132 A CN 116536132A CN 202310303900 A CN202310303900 A CN 202310303900A CN 116536132 A CN116536132 A CN 116536132A
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 23
- 229920002379 silicone rubber Polymers 0.000 title claims abstract description 21
- 239000004945 silicone rubber Substances 0.000 title claims abstract description 17
- 230000000593 degrading effect Effects 0.000 title claims abstract description 9
- 238000010998 test method Methods 0.000 title claims description 9
- 238000005070 sampling Methods 0.000 claims abstract description 14
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 239000007795 chemical reaction product Substances 0.000 claims abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 8
- 230000015556 catabolic process Effects 0.000 claims abstract description 7
- 238000006731 degradation reaction Methods 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 6
- -1 polytetrafluoroethylene Polymers 0.000 claims abstract description 6
- 239000005388 borosilicate glass Substances 0.000 claims abstract description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 239000006285 cell suspension Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 229920005830 Polyurethane Foam Polymers 0.000 claims description 7
- 230000001276 controlling effect Effects 0.000 claims description 7
- 238000006911 enzymatic reaction Methods 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 239000011496 polyurethane foam Substances 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000233866 Fungi Species 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 3
- 238000002386 leaching Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000010413 mother solution Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 239000011159 matrix material Substances 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 2
- 238000010923 batch production Methods 0.000 abstract 1
- 229920001971 elastomer Polymers 0.000 description 9
- 239000007789 gas Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 229910052717 sulfur Inorganic materials 0.000 description 3
- 239000011593 sulfur Substances 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- KPUWHANPEXNPJT-UHFFFAOYSA-N disiloxane Chemical class [SiH3]O[SiH3] KPUWHANPEXNPJT-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000006870 ms-medium Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241001135342 Phyllobacterium Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- 241000205101 Sulfolobus Species 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 108010079058 casein hydrolysate Proteins 0.000 description 1
- 235000020197 coconut milk Nutrition 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000006477 desulfuration reaction Methods 0.000 description 1
- 230000023556 desulfurization Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011982 device technology Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
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Abstract
The invention provides a reaction device for a test of degrading silicone rubber by co-metabolism flora, which comprises a bioreactor and a biological filter, wherein the bioreactor and the biological filter are both made of borosilicate glass, and a material connecting pipeline is made of polytetrafluoroethylene materials; air is filtered by an air pump input pipeline and then enters a bioreactor, and CO 2 The charge was adjusted by a flask containing KOH solution; the device is provided with a nutrition supply port and a sampling port, the MS culture medium is quantitatively introduced into the nutrition supply port, and the reaction product is collected by the sampling port. The invention is based on CO 2 The escape rule of (2) quantifies the complete degradation degree, provides operability for exploring the promotion mechanism of the primary matrix on the efficiency of the co-metabolism degradation, and canThe repeatability is strong, and is with low costs, is applicable to laboratory batch production's experimental apparatus.
Description
Technical Field
The invention relates to a test method and a reaction device for degrading silicone rubber by using flora, in particular to a test method and a reaction device for degrading silicone rubber by using co-metabolism flora, and belongs to the technical field of experimental devices.
Background
The silicon rubber material eventually faces retired problems, a large amount of power grid waste rubber causes environmental problems, physical technology is utilized to treat the rubber, resources are consumed greatly, biotechnology is utilized to find a solution favorable for the environment, meanwhile, cost is reduced, thought is provided for recycling the rubber for the power grid, biological damage of microorganisms to the rubber and rubber products is discussed in a large number of research reports in the past, so that the treatment of waste rubber is researched, people think that sulfur-consuming microorganisms are utilized to carry out desulfurization treatment on the waste rubber, sulfur bacteria, sulfolobus bacteria and rhodococcus bacteria are utilized, paper has influence on sulfur bonds of the sulfur rubber, no influence on rubber hydrocarbon is caused, and the effect is not ideal.
At present, a mature device technology can not be found to meet the technology of degrading the silicone rubber by flora.
Disclosure of Invention
The invention aims to provide a test device and a test method for degrading silicone rubber by co-metabolism flora.
In order to achieve the above object, the present invention provides the following solutions:
the technical scheme is as follows:
the utility model provides a test device of anabolism fungus crowd degradation silicone rubber, which comprises an air pump, the erlenmeyer flask, the humidifier, air filter, the bioreactor, the biofilter, air filter's input is passed through equipment connecting tube and is connected to the air pump, air filter's output is stretched into the erlenmeyer flask after passing through first needle valve and first vortex flowmeter in proper order through equipment connecting tube one way, as the input channel of erlenmeyer flask, another way is connected the humidifier through second needle valve and second vortex flowmeter through equipment connecting tube in proper order, the output channel of erlenmeyer flask and the output channel of humidifier 8 meet the back and connect the bioreactor, bioreactor and biofilter pass through equipment connecting tube and connect, KOH solution is equipped with in the erlenmeyer flask, there is the piece to adorn silicone rubber and cell suspension liquid in the bioreactor, the sample mouth is equipped with to bioreactor side top, the bioreactor top is equipped with the MS culture medium through the flow control valve connecting funnel in the funnel through the equipment connecting tube, the sample mouth is equipped with to the bioreactor top, there is the polyurethane foam piece in the biofilter, can adsorb microorganism composition and separate from in the biofilm for follow-up detection.
Furthermore, the bioreactor and the biological filter are both made of borosilicate glass, and the equipment connecting pipeline is made of polytetrafluoroethylene materials.
The second technical scheme is as follows:
a test method using the reaction device according to the first technical scheme comprises the following steps:
step 1: preparing a cell suspension according to the ratio of the mother solution of the strain to the newly prepared culture solution of 1:4-1:1, wherein the strain content is not lower than 20%, cutting the silicon rubber into small blocks, uniformly mixing the small blocks with the cell suspension, and adding the mixture into a bioreactor;
step 2: air is input through an air pump, filtered and enters the bottom of the bioreactor;
step 3: k is arranged in the device, the first needle valve 4 and the second needle valve 7 are opened in batches by an orthogonal method, and the air inflow is regulated and controlled; by controlling O 2 And CO 2 The different contents form a contrast;
step 4: controlling the input quantity of the nutrient solution by a flow control valve, and quantitatively introducing an MS culture medium into the bioreactor 13 to maintain the normal survival requirement of the strain; the gas product generated by the intermediate reaction of the enzymatic reaction is collected from a sampling port 12 above a bioreactor 13, the reaction is sampled at intervals of the same time, the content and the variety of the intermediate reaction product are recorded, the final reaction product is separated by adsorbing bacterial components through a polyurethane foam block and a biological filter, and the final reaction product is collected at a sampling port 18.
Further, the method also comprises the step 5: the liquid outlet of the bioreactor discharges the leaching liquid for analyzing the liquid components after the reaction.
By adopting the technical scheme, the invention discloses the following technical effects:
1. in the invention, the bacterial colony acquires the filtered and humidified gas through the guide pipe, supplies the needed oxygen for the fungi, and simultaneously controls the addition amount of the nutrient solution in real time, thereby realizing the full reaction of the fungi and the massive silicone rubber;
2. the method changes the oxygen inlet amount, the cell concentration, the primary matrix and the nutrient content in batches by an orthogonal method, and creates favorable and convenient conditions for inducing the multi-factor coupling corresponding relation between each reaction condition and the product type.
3. The invention is based on CO 2 The escape rule of (2) quantifies the complete degradation degree, and the exploration of the promotion mechanism of the primary matrix to the co-metabolism degradation efficiency provides operability, strong repeatability and low cost, and is suitable for experimental devices for laboratory mass production.
Drawings
FIG. 1 is a diagram of a test apparatus according to the present invention,
FIG. 2 is a schematic drawing showing the back biting of the polysiloxane-based end of the chain siloxane 1 of example 2 of the present invention,
FIG. 3 is a schematic drawing showing the back biting of the polysiloxane-based end of the chain siloxane 2 of example 2 of the present invention,
FIG. 4 is a process diagram for the degradation of an intermediate of example 2 of the present invention;
wherein the method comprises the steps of
1 air pump 2 air filter 3 borosilicate glass tube 4 first needle valve 5 first vortex flowmeter 6 conical flask 7 second needle valve 8 humidifier 9 funnel 10MS culture medium 11 flow control valve 12 sampling port 13 bioreactor 14 block silicone rubber 15 cell suspension 16 drain port 17 second vortex flowmeter 18 sampling port 19 biofilter 20 polyurethane foam block.
Detailed Description
Example 1:
a test device for degrading silicone rubber by co-metabolism flora comprises an air pump 1, a conical flask 6, a humidifier 8, an air filter 2, a bioreactor 13 and a biological filter 19, wherein the air pump 1 is connected with the input end of the air filter 2 through an equipment connecting pipeline, one path of the output end of the air filter 2 sequentially passes through a first needle valve 4 and a first vortex flowmeter 5 and then stretches into the conical flask 6 to serve as an input channel of the conical flask 6, the other path of the air filter sequentially passes through a second needle valve 7 and a second vortex flowmeter 17 and is connected with the humidifier 8 through an equipment connecting pipeline, the output channel of the conical flask 6 is connected with the output channel of the humidifier 8 after being converged, the biological reactor 13 and the biological filter 19 are connected through an equipment connecting pipeline, KOH solution is filled in the conical flask 6, the biological reactor 13 is provided with a block-shaped silicone rubber 14 and a cell suspension 15 liquid, a sampling port 12 is reserved above the side of the biological reactor 13, the biological reactor 13 is sequentially connected with a funnel 9 through an equipment connecting pipeline through a flow control valve 11, an MS medium 10 is filled in the funnel 9, a liquid discharge port 16 is arranged below the biological reactor 13, and a foam block 20 is reserved above the biological filter 19.
The air pump 1 in the device continuously provides air flowing in a directional way, the air is input into the equipment connecting pipeline through the air pump 1, impurities are filtered and filtered through the air filter 2, one path of the air enters the bioreactor through the conical flask 6, and KOH solution in the conical flask 6 regulates CO2 filling amount; the other path controls the amount of water vapor flowing through a humidifier 8, the first needle valve 4 and the second needle valve 7 are used for adjusting the air charging amount, and the first vortex flowmeter 5 and the second vortex flowmeter 17 are used for monitoring the air charging amount; the two paths of air are converged and then enter the bottom of the bioreactor 13, so that the air fully participates in the enzymatic reaction in the bioreactor from top to bottom. The bioreactor 13 and the biological filter 19 are both made of borosilicate glass, and the equipment connecting pipeline is made of polytetrafluoroethylene materials.
In the device, MS culture medium is quantitatively introduced through a funnel 9, intermediate reaction products are collected through a sampling port 12, and leaching liquid flows out through a liquid outlet 16.
The MS culture medium has higher inorganic salt concentration, can ensure mineral nutrition required by tissue growth and can accelerate the growth of callus. Because of the high ion concentration in the formula, even if some components are slightly in and out during the processes of preparation, storage, disinfection and the like, the balance among ions can not be influenced. The MS solid culture medium can be used for inducing callus, and can also be used for culturing embryo, stem segment, stem tip and anther, and the liquid culture medium can be used for cell suspension culture with obvious success. The amount and proportion of inorganic nutrients of the MS culture medium are proper, and the inorganic nutrients are sufficient to meet the nutritional and physiological requirements of plant cells. Therefore, in general, it is not necessary to add any additional organic components such as amino acids, casein hydrolysate, yeast extract, and coconut milk. The high nitrate, potassium and ammonium content of the MS medium compared to the basic components of other media is a significant feature. The purified streptomycete, xanthomonas or phyllobacterium roseum is enriched in a bioreactor, and is subjected to enzymatic reaction with waste silicone rubber particles under the condition of introducing filtered air, and nutrient solution is quantitatively added by controlling a flow control valve to regulate and control the reaction in an MS culture medium. Through the reaction in the bioreactor, the directional flowing gas enters the biological filter, wherein the polyurethane foam block has hydrophobicity and can adsorb various particles generated by the enzymatic reaction, and the immobilized product is convenient for identification and analysis.
Example 2:
a test method using the test device of example 1, comprising the steps of:
step 1: preparing a cell suspension according to the ratio of the mother solution of the strain to the newly prepared culture solution of 1:4-1:1, wherein the strain content is not lower than 20%, cutting the silicon rubber into small blocks, uniformly mixing the small blocks with the cell suspension, and adding the mixture into a bioreactor;
step 2: air is input through the air pump 1, filtered and enters the bottom of the bioreactor 13;
step 3: KOH is arranged in the device, the first needle valve 4 and the second needle valve 7 are opened in batches by an orthogonal method, and the air inflow is regulated and controlled; by controlling O 2 And CO 2 The different contents are compared, the air is input from the bioreactor 13, the air flows from the lower part to the upper part,fully participate in the enzymatic reaction in the bioreactor, and the enzyme in the reaction device is generated by bacterial colony;
step 4: controlling the input quantity of the nutrient solution by a flow control valve, and quantitatively introducing an MS culture medium into the bioreactor 13 to maintain the normal survival requirement of the strain; the gas product generated by the intermediate reaction of the enzymatic reaction is collected from a sampling port 12 above a bioreactor 13, the reaction is sampled at intervals of the same time, the content and the variety of the intermediate reaction product are recorded, the final reaction product passes through a conduit and a polyurethane foam block to adsorb and separate microorganisms, and the final gas product is collected at a sampling port 18;
step 5: the liquid outlet 16 of the bioreactor 13 discharges the leachate for analysis of the liquid composition after the reaction.
Chemical reaction equation/scheme for reaction:
proper amount of CO 2 The conditions are as follows: 2KOH+CO 2 ＝K 2 CO 3 +H 2 O
Excess CO 2 The conditions are as follows: KOH+CO 2 ＝KHCO 3 。
Claims (4)
1. The utility model provides a test device of anabolism fungus crowd degradation silicone rubber, a serial communication port, including air pump (1), erlenmeyer flask (6), humidifier (8), air cleaner (2), bioreactor (13), biofilter (19), air pump (1) are through equipment connecting tube connect the input of air cleaner (2), the output of air cleaner (2) stretches into the input passageway of erlenmeyer flask (6) after passing through first needle valve (4) and first vortex flowmeter (5) in proper order all the way through equipment connecting tube, another way is through equipment connecting tube connect humidifier (8) through second needle valve (7) and second vortex flowmeter (17) in proper order, output passageway and humidifier (8) of erlenmeyer flask (6) meet back and connect bioreactor (13), bioreactor (13) and biofilter (19) are through equipment connecting tube connect, KOH solution is equipped with in the erlenmeyer flask (6), there are piece dress silicone rubber (14) and cell suspension (15) in bioreactor (13), biological reactor (13) side top is left and is equipped with funnel (9) in the side top through funnel (9) in the connecting tube way through funnel (9), a liquid outlet (16) is arranged below the bioreactor (13), a sampling port is reserved above the biological filter (19), and a polyurethane foam block (20) is arranged in the biological filter (19).
2. The test device for degrading silicone rubber by co-metabolizing bacteria according to claim 1, wherein the bioreactor (13) and the biological filter (19) are made of borosilicate glass, and the equipment connecting pipeline is made of polytetrafluoroethylene materials.
3. A test method using the reaction apparatus of claim 1, comprising the steps of:
step 1: preparing a cell suspension according to the ratio of the mother solution of the strain to the newly prepared culture solution of 1:4-1:1, wherein the strain content is not lower than 20%, cutting the silicon rubber into small blocks, uniformly mixing the small blocks with the cell suspension, and adding the small blocks into a bioreactor (13);
step 2: air is input through an air pump (1), filtered and enters the bottom of a bioreactor (13);
step 3: KOH is arranged in the device, the first needle valve (4) and the second needle valve (7) are opened in batches by an orthogonal method, and the air inflow is regulated and controlled; by controlling O 2 And CO 2 The different contents form a contrast;
step 4: controlling the input quantity of the nutrient solution by a flow control valve, and quantitatively introducing an MS culture medium into a bioreactor (13) to maintain the normal survival requirement of strains; the gas product generated by the intermediate reaction of the enzymatic reaction is collected from a sampling port (12) above a bioreactor (13), the reaction is sampled at intervals of the same time, the content and the variety of the intermediate reaction product are recorded, the final reaction product is collected at the sampling port (12), the final reaction product is separated by adsorbing bacterial components through a polyurethane foam block and a biological filter, and the final reaction product is collected at a sampling port (18).
4. A test method according to claim 3, further comprising step 5: the liquid outlet (16) of the bioreactor (13) discharges the leaching liquid for analyzing the liquid components after the reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310303900.2A CN116536132A (en) | 2023-03-27 | 2023-03-27 | Test method and reaction device for degrading silicone rubber by co-metabolism flora |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310303900.2A CN116536132A (en) | 2023-03-27 | 2023-03-27 | Test method and reaction device for degrading silicone rubber by co-metabolism flora |
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| CN116536132A true CN116536132A (en) | 2023-08-04 |
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| CN202310303900.2A Pending CN116536132A (en) | 2023-03-27 | 2023-03-27 | Test method and reaction device for degrading silicone rubber by co-metabolism flora |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104946527A (en) * | 2015-06-26 | 2015-09-30 | 青岛理工大学 | Culturing device for formaldehyde degradation bacteria |
| CN111154647A (en) * | 2020-01-17 | 2020-05-15 | 英诺维尔智能科技（苏州）有限公司 | Closed circulation external exchange biological product reactor |
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- 2023-03-27 CN CN202310303900.2A patent/CN116536132A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104946527A (en) * | 2015-06-26 | 2015-09-30 | 青岛理工大学 | Culturing device for formaldehyde degradation bacteria |
| CN111154647A (en) * | 2020-01-17 | 2020-05-15 | 英诺维尔智能科技（苏州）有限公司 | Closed circulation external exchange biological product reactor |
Non-Patent Citations (1)
| Title |
|---|
| YANG等, FRONT. MATER. /MONOMER RECOVERY AND NANO-SILICA SEPARATION FROM BIODEGRADED WASTE SILICONE RUBBER SHED OF COMPOSITE INSULATOR, pages 3 * |
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