CN116520014A - Multichannel nanopore measurement circuit - Google Patents

Multichannel nanopore measurement circuit Download PDF

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Publication number
CN116520014A
CN116520014A CN202310480340.8A CN202310480340A CN116520014A CN 116520014 A CN116520014 A CN 116520014A CN 202310480340 A CN202310480340 A CN 202310480340A CN 116520014 A CN116520014 A CN 116520014A
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CN
China
Prior art keywords
circuit
switch
nanopore
multiplexing
channel
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Pending
Application number
CN202310480340.8A
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Chinese (zh)
Inventor
刘炬坪
周智
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Shenzhen Merrime Nanopore Technology Co ltd
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Shenzhen Merrime Nanopore Technology Co ltd
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Priority to CN202310480340.8A priority Critical patent/CN116520014A/en
Publication of CN116520014A publication Critical patent/CN116520014A/en
Pending legal-status Critical Current

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    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K17/00—Electronic switching or gating, i.e. not by contact-making and –breaking
    • H03K17/002—Switching arrangements with several input- or output terminals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R19/00—Arrangements for measuring currents or voltages or for indicating presence or sign thereof
    • G01R19/25—Arrangements for measuring currents or voltages or for indicating presence or sign thereof using digital measurement techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
    • Y02E60/10—Energy storage using batteries

Abstract

The invention discloses a multichannel nanopore measurement circuit, which comprises a gating control circuit and an analog-to-digital conversion circuit, wherein the gating control circuit comprises a controller and at least one first multiplexing switch, two ends of each switch in the first multiplexing switch are respectively connected with the analog-to-digital conversion circuit and a nanopore channel, and the controller is connected with the first multiplexing switch and the analog-to-digital conversion circuit to control each switch in the first multiplexing switch to be sequentially and circularly conducted in a time interval mode and collect currents of multiple nanopore channels. Compared with the prior art, the invention adopts a time-sharing multiplexing mode to gate the switch in the first multiplexing switch so as to lead the multi-path switch to be staggered and conducted, and the induced currents of the multi-path nanopore channel are circularly collected at different times in sequence, so that the current collection of the multi-path nanopore channel can be realized by using the same analog-digital conversion circuit and gating control circuit, and the circuit has simple structure and lower cost.

Description

Multichannel nanopore measurement circuit
Technical Field
The invention relates to a multichannel nanopore measurement circuit.
Background
In the prior art, biological solution is generally poured into a biochip tank to measure nano holes in the biochip tank, and because the biological solution is charged ionic solution, two electrodes of a cathode and an anode are needed to be used at the upper end and the lower end of the tank, DA voltages are applied to the two electrodes, related substances pass through the nano holes when moving from a cathode to an anode under the action of an electrode electric field, induced current is generated, and the composition of the related substances can be distinguished by detecting the current.
In a biochip, hundreds of nanopore channels are usually arranged, in theory, independent material composition information can be obtained by sampling the current of any one nanopore channel, and massive material composition information can be obtained in extremely short time by fusing sequencing data of hundreds of nanopore channels. At present, by copying hundreds to thousands of single nanopore measurement circuits to measure hundreds to thousands of different nanopore channels respectively, high-throughput measurement is realized, however, 100 identical circuit structures are needed for one hundred nanopores, although the circuits are easy to transplant and expand, the circuits are quite many, interference is easy to generate among the circuits, and the schematic diagram is drawn with a lot of pages, so that the PCB is extremely difficult to wire, a single circuit board cannot be accommodated generally, a plurality of circuit boards are needed, high cost and extremely long hardware iteration time of design links and manufacturing links are caused, and the whole measurement circuit is huge in volume.
Disclosure of Invention
The invention aims to solve the technical problem of providing a multichannel nanopore measurement circuit which has a simple circuit structure and low cost and can reduce interference.
In order to solve the technical problems, the invention provides a multichannel nanopore measurement circuit, which comprises a gating control circuit and an analog-to-digital conversion circuit, wherein the gating control circuit comprises a controller and at least one first multiplexing switch, two ends of each switch in the first multiplexing switch are respectively connected with the analog-to-digital conversion circuit and a nanopore channel, and the controller is connected with the first multiplexing switch and the analog-to-digital conversion circuit to control each switch in the first multiplexing switch to be sequentially and circularly conducted in a time interval mode and collect currents of the multichannel nanopore channels.
The further technical scheme is as follows: the multichannel nanopore measurement circuit further comprises at least one sensing conversion circuit, each sensing conversion circuit comprises a first operational amplifier, wherein the in-phase input end and the anti-phase input end of the first operational amplifier are respectively connected with a DA power supply and a nanopore channel, and the output end of the first operational amplifier is connected with one switch of a first multiplexing switch.
The further technical scheme is as follows: the multichannel nanopore measurement circuit further comprises a first operational amplifier circuit connected between the first multiplexing switch and the analog-to-digital conversion circuit.
The further technical scheme is as follows: the multichannel nanopore measurement circuit further comprises a measurement signal generation circuit, the measurement signal generation circuit comprises a signal generation circuit and a digital-to-analog conversion circuit which are sequentially connected, the multichannel nanopore measurement circuit further comprises at least one gating circuit connected with the output end of the digital-to-analog conversion circuit, the gating circuit comprises at least one second multiplexing switch, two ends of each switch in the second multiplexing switch are respectively connected with the digital-to-analog conversion circuit and a nanopore channel, and the controller is connected with the second multiplexing switch to control each switch in the second multiplexing switch to be sequentially and circularly conducted in a time-division mode, so that DA voltage is provided for the multichannel nanopore channels.
The further technical scheme is as follows: the measuring signal generating circuit further comprises a holding circuit, the holding circuit comprises a plurality of capacitors, one end of each capacitor is connected between one switch in the second multiplexing switch and the nanopore channel connected with the switch, and the other end of each capacitor is grounded.
The further technical scheme is as follows: the measuring signal generating circuit further comprises a second operational amplifier circuit connected between the digital-to-analog conversion circuit and the second multiplexing switch.
The further technical scheme is as follows: the signal generating circuit is also connected with the controller to form a measurement signal according to the signal from the controller.
The further technical scheme is as follows: the first multiplexing switch and the second multiplexing switch are multiplexing chips with the model number of ADG 1208.
The further technical scheme is as follows: the number of the gating control circuit, the analog-to-digital conversion circuit and the gating circuit is one, the gating control circuit comprises a first multiplexing switch, and the gating circuit comprises a second multiplexing switch.
Compared with the prior art, the multi-channel nanopore measurement circuit is provided with the gating control circuit, the gating control circuit is provided with the first multiplexing switch, each switch in the first multiplexing switch is connected with one nanopore channel and the input end of the analog-to-digital conversion circuit, the output end of the analog-to-digital conversion circuit and the first multiplexing switch are both connected with the controller, each switch in the first multiplexing switch is controlled by the controller to be circularly conducted in a time-division mode in sequence, meanwhile, the current of the multiple nanopore channels collected by the analog-to-digital conversion circuit is detected by the controller, namely, the switch in the first multiplexing switch is gated in a time-division multiplexing mode, so that the multiple switches are conducted in a staggered mode, the induced current of the multiple nanopore channels is circularly collected in different time sequences, the current collection of the multiple nanopore channels can be realized by using the same analog-to-digital conversion circuit and the gating control circuit, the circuit structure is simple, a large number of analog-to-digital chip group circuits with the same structure are not needed in the measurement of the multichannel nanopore measurement circuit, and the problems of difficult graph routing, huge overall size and mutual interference caused by circuit lines in the traditional multichannel nanopore measurement circuit are fundamentally solved.
Drawings
Fig. 1 is a schematic circuit structure diagram of a first embodiment of a multi-channel nanopore measurement circuit of the present invention.
FIG. 2 is a schematic diagram of current collection for each nanopore channel in a multichannel nanopore measurement circuit of the present invention.
Fig. 3 is a schematic circuit structure diagram of a second embodiment of the multi-channel nanopore measurement circuit of the present invention.
Detailed Description
The present invention will be further described with reference to the drawings and examples below in order to more clearly understand the objects, technical solutions and advantages of the present invention to those skilled in the art.
Referring to fig. 1, fig. 1 is a schematic circuit diagram of a first embodiment of a multi-channel nanopore measurement circuit 100 according to the present invention. In the embodiment shown in the drawings, the multi-channel nanopore measurement circuit 100 of the present invention includes a gating control circuit and an analog-to-digital conversion circuit 23, where the gating control circuit includes a controller 211 and at least one first multiplexing switch 212, two ends of each switch in the first multiplexing switch 212 are respectively connected to an input end of the analog-to-digital conversion circuit 23 and a nanopore channel, and the controller 211 is connected to an output end of the analog-to-digital conversion circuit 23 and the first multiplexing switch 212, so as to control each switch in the first multiplexing switch 212 to be circularly conducted in a time-division manner in sequence, and collect currents of multiple nanopore channels. Preferably, in this embodiment, the controller 211 may use a control chip such as a single-chip microcomputer, an ARM or a DSP, so as to precisely control the switching on of the switch at the moment.
Specifically, in this embodiment, the number of the gating control circuit and the analog-to-digital conversion circuit 23 is one, and the gating control circuit includes a first multiplexing switch 212, that is, in this embodiment, the multiple nanopore channels share a controller 211, a first multiplexing switch 212 and an analog-to-digital conversion circuit 23, and the sensing current collection of the multiple nanopore channels can be achieved only by sequentially and circularly staggering the switches in the first multiplexing switch 212 according to the control signal of the controller 211, thereby achieving the measurement of the multiple nanopore channels. It will be appreciated that the number of gating control circuits and analog-to-digital conversion circuits 23 and/or the first multiplexing switches 212 may be increased according to the number of nanopore channels, i.e. the number of nanopore channels sharing one gating control circuit and analog-to-digital conversion circuit 23 depends on the chip performance, for example, N nanopore channels time-division multiplex 1-channel analog-to-digital conversion circuits 23 (AD), meaning that after multiplexing, the total sampling rate of the total channels is N times the original respective sampling rate, the total sampling rate cannot exceed the sampling rate of the AD, assuming that any one of the nanopore channels needs a sampling rate of 1KSPS, and that 6 pore channels multiplexing needs a total sampling rate of greater than or equal to 6KSPS, and when the total sampling rate of the AD chip is insufficient, hundreds of pore channels may also be divided into groups, each group multiplexing the same gating control circuit and analog-to-digital conversion circuit 23, for example, if there are 1000 nanopore channels, each group sharing the same gating control circuit and analog-to-digital conversion circuit 23, and wherein the nanopore channels in the group may also be divided into ten groups, each group sharing the same gating control circuit and the same analog-to-digital conversion circuit 23, and the nanopore channels in the group may be multiplexed into a small group, and each group sharing a small group of the first multiplexing switches 212, which has a low cost and a simple structure.
In some embodiments, the multi-channel nanopore measurement circuit 100 further includes three sensing conversion circuits 24, each of the sensing conversion circuits 24 includes a first operational amplifier U1, wherein a non-inverting input terminal of the first operational amplifier U1 is connected to the DA power source V, an inverting input terminal of the first operational amplifier U1 is connected to a nanopore channel, and is grounded through the nanopore channel, and an output terminal of the first operational amplifier U1 is connected to one of the first multiplexing switches 212. Further, a second capacitor C2 is connected between the inverting input terminal of the first operational amplifier U1 and the output terminal thereof.
Preferably, in this embodiment, the multi-channel nanopore measurement circuit 100 further includes a first operational amplifier 22 connected between the first multiplexing switch 212 and the analog-to-digital conversion circuit 23. It is to be understood that the analog-to-digital conversion circuit 2312 and the first operational amplifier circuit 22 are respectively an analog-to-digital conversion circuit and an operational amplifier circuit commonly used by those skilled in the art, and will not be described herein.
In this embodiment, the first multiplexing switch 212 uses a multiplexing chip with the model ADG1208, and in some other embodiments, other multiplexing chips (such as a multiplexing chip with the model CD 4053) that can implement the same multiplexing function may also be used to implement time-division multiplexing to collect the current of the nanopore channel in cooperation with the controller 211. As shown in fig. 1, in the present embodiment, the switch S11, the switch S21 and the switch S31 in the first multiplexing switch 212 are all connected to the controller 211, and the switch S11 is connected to the analog-to-digital conversion circuit 23 through the first operational amplifier 22 and connected to the nanopore channel CH1 through the sensing conversion circuit 24, the switch S21 is connected to the analog-to-digital conversion circuit 23 through the first operational amplifier 22 and connected to the nanopore channel CH2 through the sensing conversion circuit 24, the switch S31 is connected to the analog-to-digital conversion circuit 23 through the first operational amplifier 22 and connected to the nanopore channel CH3 through the sensing conversion circuit 24, that is, the sensing currents of different nanopore channels are collected through the cooperation of the switch S11, the switch S21, the switch S31, the controller 211 and the analog-to-digital conversion circuit 23, thus, the nanopore measurement is realized, when the switch S11, the switch S21 and the switch S31 are sequentially and circularly conducted in time intervals, the current collection situation in the nanopore channel CH1, the nanopore channel CH2 and the nanopore channel CH3 is shown in fig. 2, square wave positions (protruding positions) in the drawing are respectively the collection moments of the nanopore channel CH1, the nanopore channel CH2 and the nanopore channel CH3, straight line positions are idle moments, and if the collection moment and the idle moment of each nanopore channel are the same, the collection period is 3 (collection moment+idle moment), and it is known that the respective collection behaviors of the multiple nanopore channels occur at different moments in sequence.
Referring to fig. 3, fig. 3 is a schematic circuit diagram of a second embodiment of a multi-channel nanopore measurement circuit 100 according to the present invention. The present embodiment is different from the first embodiment in that a measurement signal generating circuit is added in the present embodiment, and the rest of the circuit structures are the same or similar. In this embodiment, the multi-channel nanopore measurement circuit 100 further includes a measurement signal generating circuit, where the measurement signal generating circuit includes a signal generating circuit 11 and a digital-to-analog conversion circuit 12 that are sequentially connected, and further includes at least one gating circuit connected to an output end of the digital-to-analog conversion circuit 12, where the gating circuit includes at least one second multiplexing switch 14, two ends of each of the second multiplexing switches 14 are respectively connected to the digital-to-analog conversion circuit 12 and a nanopore channel, and the controller 211 is connected to the second multiplexing switches 14 to control each of the second multiplexing switches 14 to be sequentially turned on in a period-division cycle, so as to provide a DA voltage for measurement of the nanopore channel. It can be understood that, in the multi-channel nanopore measurement circuit 100 of the present embodiment, the sensing conversion circuit 24 is disposed, and the second multiplexing switch 14 provides the DA voltage to the sensing conversion circuit 24 connected to the nanopore channel, so as to measure the nanopore channel, that is, the second multiplexing switch 14 is connected to the non-inverting input terminal of the first operational amplifier U1 in the sensing conversion circuit 24 to provide the DA voltage as the DA power supply.
In this embodiment, the number of the gating circuits is one, and the gating circuits include a second multiplexing switch 14, that is, in this embodiment, the multiplexing nanopore channels share one second multiplexing switch 14 and the digital-to-analog conversion circuit 12, and voltage supply to the multiplexing nanopore channels can be achieved only by staggering and turning on the switches in the second multiplexing switch 14 in sequence according to the control signal of the controller 211. It will be appreciated that in some other embodiments, the number of gating circuits and/or second multiplexing switches 14 may be increased according to the number of nanopore channels, i.e. when multiplexing of too many nanopore channels is not enough, the number of gating circuits and/or second multiplexing switches 14 may be increased, and multiple nanopore channels may be divided into multiple groups, where each group multiplexes the same gating circuits and/or second multiplexing switches 14 when there are 1000 nanopore channels to provide DA voltages for measurement, for example, each group may be divided into ten groups, each group has 100 nanopore channels sharing the same gating circuit, and each group may further be subdivided into small groups, each small group shares one second multiplexing switch 14, which may save resources, without setting one signal generating circuit 11 and digital-to-analog conversion circuit 12 for one nanopore channel, without setting a large number of chip group circuits with the same structure, which fundamentally solves the problems of huge number of chips, huge volume, and signal interference between DA chips in the existing measurement signal generating circuit, and has a simple circuit structure and low cost.
Further, the measurement signal generating circuit further includes a second operational amplifier circuit 13 connected between the digital-to-analog conversion circuit 12 and the second multiplexing switch 14, and in this embodiment, the signal generating circuit 11 is further connected to the controller 211 to form a measurement signal according to a signal from the controller 211. It is understood that the signal generating circuit 11, the digital-to-analog conversion circuit 12 and the second operational amplifier circuit 13 are all commonly used circuit modules by those skilled in the art, and will not be described herein.
Preferably, in this embodiment, the second multiplexing switch 14 is also a multiplexing chip with the model ADG1208, and is matched with the controller 211 to realize time-division multiplexing of the measurement signals, as shown in fig. 3, in the second multiplexing switch 14, the switch S12, the switch S22 and the switch S32 are all connected with the controller 211, and the switch S12 is connected with the second operational amplifier 13 and the in-phase input terminal of the first operational amplifier U1 connected with the nanopore channel CH1, the switch S22 is connected with the second operational amplifier 13 and the in-phase input terminal of the first operational amplifier U1 connected with the nanopore channel CH2, the switch S32 is connected with the second operational amplifier 13 and the in-phase input terminal of the first operational amplifier U1 connected with the nanopore channel CH3, in this embodiment, after passing through the digital-analog conversion circuit 12 and the second operational amplifier 13, the switch S22 and the switch S32 in the circuit respectively, the measurement signals generated in the signal generating circuit 11 can be provided to the nanopore channel CH1, the nanopore channel CH2 and the nanopore channel CH3 through the switch S12, the switch S22 and the switch S32, and the voltage can be provided by the digital-analog-to-analog conversion circuit 13 and the nanopore channel CH3, and the voltage can be provided by the signal generating circuit DA and the nanopore channel CH3 in sequence, and the time-division multiplexing channel can be realized when the measurement signal is realized.
In some embodiments, the measurement signal generating circuit further includes a holding circuit, where the holding circuit includes a plurality of capacitors C, one end of each capacitor C is connected between one switch of the second multiplexing switch 14 and the non-inverting input terminal of the first operational amplifier U1 connected to the one switch, and the other end is grounded. Based on the above design, the voltage output can be continued to be maintained by the capacitor C when the switch in the second multiplexing switch 14 is turned off. It will be appreciated that in certain other embodiments, the retention circuit may also be implemented by a retention chip such as lf 398; or may be implemented by a multiplexing chip integrated with multiplexing functions and voltage holding functions.
In summary, the multi-channel nanopore measurement circuit is provided with the gating control circuit, the gating control circuit is provided with the first multiplexing switch, each switch in the first multiplexing switch is connected with a nanopore channel and an analog-to-digital conversion circuit, the analog-to-digital conversion circuit and the first multiplexing switch are connected with the controller, each switch in the first multiplexing switch is controlled by the controller to be circularly conducted in a time-division mode in sequence, meanwhile, the current of the multiple nanopore channels collected by the analog-to-digital conversion circuit is detected by the controller, namely, the switch in the first multiplexing switch is gated in a time-division multiplexing mode, so that the multiple switches are conducted in a staggered mode, the induced currents of the multiple nanopore channels are circularly collected at different times in sequence, the current collection of the multiple nanopore channels can be realized by using the same analog-to-digital conversion circuit and the gating control circuit, the circuit structure is simple, a large number of analog-to-digital chip group circuits with the same structure are not needed in the measurement of the multiple nanopore channels, the cost is low, and the problems of difficult wiring of a schematic diagram, huge whole volume and mutual interference caused by more circuit lines in the existing multi-channel nanopore measurement circuit are fundamentally solved; and the device is also provided with a measuring signal generating circuit for providing DA voltage for the measurement of the multichannel nanopore channels in a time-sharing multiplexing mode, so that the circuit of the whole multichannel nanopore measuring circuit can be further reduced, the development of miniaturized equipment such as palm equipment for multichannel nanopore measurement becomes possible, and the mobile portability of the measuring equipment loaded with the multichannel nanopore measuring circuit is facilitated.
The foregoing is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Various equivalent changes and modifications can be made by those skilled in the art based on the above embodiments, and all equivalent changes or modifications made within the scope of the claims shall fall within the scope of the present invention.

Claims (9)

1. A multichannel nanopore measurement circuit is characterized in that: the device comprises a gating control circuit and an analog-to-digital conversion circuit, wherein the gating control circuit comprises a controller and at least one first multiplexing switch, two ends of each switch in the first multiplexing switch are respectively connected with the analog-to-digital conversion circuit and a nanopore channel, and the controller is connected with the first multiplexing switch and the analog-to-digital conversion circuit to control each switch in the first multiplexing switch to be circularly conducted in a time-interval mode in sequence and collect currents of the nanopore channels in multiple ways.
2. The multi-channel nanopore measurement circuit of claim 1, wherein: the multichannel nanopore measurement circuit further comprises at least one sensing conversion circuit, each sensing conversion circuit comprises a first operational amplifier, wherein the in-phase input end and the anti-phase input end of the first operational amplifier are respectively connected with a DA power supply and a nanopore channel, and the output end of the first operational amplifier is connected with one switch of a first multiplexing switch.
3. The multi-channel nanopore measurement circuit of claim 1, wherein: the multichannel nanopore measurement circuit further comprises a first operational amplifier circuit connected between the first multiplexing switch and the analog-to-digital conversion circuit.
4. A multi-channel nanopore measurement circuit according to any of claims 1-3, wherein: the multichannel nanopore measurement circuit further comprises a measurement signal generation circuit, the measurement signal generation circuit comprises a signal generation circuit and a digital-to-analog conversion circuit which are sequentially connected, the multichannel nanopore measurement circuit further comprises at least one gating circuit connected with the output end of the digital-to-analog conversion circuit, the gating circuit comprises at least one second multiplexing switch, two ends of each switch in the second multiplexing switch are respectively connected with the digital-to-analog conversion circuit and a nanopore channel, and the controller is connected with the second multiplexing switch to control each switch in the second multiplexing switch to be sequentially and circularly conducted in a time-division mode, so that DA voltage is provided for the multichannel nanopore channels.
5. The multi-channel nanopore measurement circuit according to claim 4, wherein: the measuring signal generating circuit further comprises a holding circuit, the holding circuit comprises a plurality of capacitors, one end of each capacitor is connected between one switch in the second multiplexing switch and the nanopore channel connected with the switch, and the other end of each capacitor is grounded.
6. The multi-channel nanopore measurement circuit according to claim 4, wherein: the measuring signal generating circuit further comprises a second operational amplifier circuit connected between the digital-to-analog conversion circuit and the second multiplexing switch.
7. The multi-channel nanopore measurement circuit according to claim 4, wherein: the signal generating circuit is also connected with the controller to form a measurement signal according to the signal from the controller.
8. The multi-channel nanopore measurement circuit according to claim 4, wherein: the first multiplexing switch and the second multiplexing switch are multiplexing chips with the model number of ADG 1208.
9. The multi-channel nanopore measurement circuit according to claim 4, wherein: the number of the gating control circuit, the analog-to-digital conversion circuit and the gating circuit is one, the gating control circuit comprises a first multiplexing switch, and the gating circuit comprises a second multiplexing switch.
CN202310480340.8A 2023-04-28 2023-04-28 Multichannel nanopore measurement circuit Pending CN116520014A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310480340.8A CN116520014A (en) 2023-04-28 2023-04-28 Multichannel nanopore measurement circuit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310480340.8A CN116520014A (en) 2023-04-28 2023-04-28 Multichannel nanopore measurement circuit

Publications (1)

Publication Number Publication Date
CN116520014A true CN116520014A (en) 2023-08-01

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