CN116519692A - Microscopic device for identifying traditional Chinese medicine components and application method thereof - Google Patents
Microscopic device for identifying traditional Chinese medicine components and application method thereof Download PDFInfo
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- CN116519692A CN116519692A CN202310683870.2A CN202310683870A CN116519692A CN 116519692 A CN116519692 A CN 116519692A CN 202310683870 A CN202310683870 A CN 202310683870A CN 116519692 A CN116519692 A CN 116519692A
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- 239000003814 drug Substances 0.000 title claims abstract description 102
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- 238000005286 illumination Methods 0.000 claims abstract description 10
- 238000000386 microscopy Methods 0.000 claims abstract description 8
- RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 claims description 56
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- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 claims description 15
- 238000007599 discharging Methods 0.000 claims description 12
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- 239000000203 mixture Substances 0.000 claims description 11
- 239000011521 glass Substances 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 8
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- 238000004458 analytical method Methods 0.000 claims description 4
- 239000012258 stirred mixture Substances 0.000 claims description 4
- 239000007789 gas Substances 0.000 description 51
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application discloses a microscopic device for identifying traditional Chinese medicine components and a using method thereof, relates to the technical field of traditional Chinese medicine identification, and comprises the following steps: the micro-mechanism is fixedly arranged at one side of the top end of the base, and a plurality of supporting feet are arranged at the bottom end of the base; the traditional Chinese medicine treatment mechanism is arranged on the other side of the top end of the base, a sample moving mechanism is arranged between the traditional Chinese medicine treatment mechanism and the microscopic mechanism, and an exhaust pipe is arranged on the traditional Chinese medicine treatment mechanism; an illumination mechanism disposed in the base and corresponding to a position of the microscopy mechanism; the condenser ring is rotatably arranged on the microscopic mechanism; the control panel is fixedly arranged on the traditional Chinese medicine treatment mechanism. According to the invention, the manual operation error is reduced, and the sample processing efficiency and safety are improved, so that the accuracy and convenience of the identification of the traditional Chinese medicine components are effectively improved.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine identification, in particular to a microscopic device for identifying traditional Chinese medicine components and a use method thereof.
Background
Traditional Chinese medicine is used as traditional medical treatment means and still has wide application in the modern medical field, for the identification of traditional Chinese medicine components, inspection analysis is usually needed through a microscopic technology, a chloral hydrate permeabilization film preparation method is a commonly used microscopic film preparation method and is mainly applied to microscopic identification of plant medicines, for example, an operator needs to manually add, mix and heat in a well ventilated environment aiming at a reagent with toxicity and pungent smell of chloral hydrate, as chloral hydrate has stronger pungent smell, the operator can be uncomfortable in the treatment process and even affects health, meanwhile, the manual operation also increases the risks of reagent misoperation and sample pollution, in addition, the operation complexity of the traditional microscopic device is higher in the traditional Chinese medicine component identification process, and a plurality of steps are needed to be manually intervened for sample treatment and observation, for example, the traditional microscopic device generally needs to manually prepare a traditional Chinese medicine sample and then place the traditional Chinese medicine sample on a stage, and observe the sample through adjusting parameters such as focal length of a microscope, in addition, the pretreatment process (such as grinding, mixing and the like) of the traditional Chinese medicine sample also needs to be manually operated, and the operation and the human resources and the sample can not be polluted only because of human resources and human resources are consumed;
the operator contacts the risk of poisonous and pungent smell in the handling, and the operational safety is lower, and maloperation and sample pollution's possibility is great simultaneously, and traditional micromanipulator is in the identification of in-process medicine composition in addition, because need many times manual intervention, makes whole operation flow become loaded down with trivial details inefficiency easily, simultaneously, because the error probably appears in the manual handling in-process, has influenced reliability and the accuracy of sample, consequently, need to develop a traditional chinese medicine composition identification micromanipulator that can realize automation mechanized operation, simplify operation flow, improve identification efficiency and accuracy.
Disclosure of Invention
The application provides a microscopic device for identifying traditional Chinese medicine components and a using method thereof, and mainly aims to solve the problems of complex operation, lower safety, misoperation and larger sample pollution risk.
To achieve the above object, a first aspect of the present application provides a microscopic device for identifying components of a traditional Chinese medicine and a method for using the same, including:
the micro-mechanism is fixedly arranged at one side of the top end of the base, and a plurality of supporting feet are arranged at the bottom end of the base;
the traditional Chinese medicine treatment mechanism is arranged on the other side of the top end of the base, a sample moving mechanism is arranged between the traditional Chinese medicine treatment mechanism and the microscopic mechanism, and an exhaust pipe is arranged on the traditional Chinese medicine treatment mechanism;
an illumination mechanism disposed in the base and corresponding to a position of the microscopy mechanism;
the condenser ring is rotatably arranged on the microscopic mechanism;
the control panel is fixedly arranged on the traditional Chinese medicine treatment mechanism.
In a possible embodiment, the microscopy mechanism comprises: the coarse focusing spiral adjusting mechanism is arranged at one side of the top end of the base, and a telescopic movable bracket is arranged on the coarse focusing spiral adjusting mechanism; the display is fixedly arranged on the front side of the movable support, a lens barrel is further arranged below the display, a rotatable switching eyepiece assembly is arranged at the bottom end of the lens barrel, and the lens barrel corresponds to the illumination mechanism in position.
In one possible embodiment, the chinese medicine treatment mechanism comprises: the box body is fixedly arranged at one side, far away from the microscopic mechanism, of the top end of the base, the box body is in a cavity state, and an exhaust pipe is arranged on the outer wall of the box body; the partition board is fixedly arranged in the inner cavity of the box body and divides the box body into a material processing cavity and a gas purifying cavity; the impurity filter screen and the gas purification assembly are fixedly arranged in the gas purification cavity, and the gas purification assembly is further arranged on one side, close to the exhaust pipe, of the impurity filter screen; the negative pressure vacuum pump is fixedly arranged at the top end of the box body and is connected with the exhaust pipe; the crushing assembly is fixedly arranged on the end face of the top end of the box body of the material processing cavity, the mixing tank is fixedly arranged at the bottom end of the material processing cavity far away from one end of the crushing assembly, and a magnetic stirrer is arranged in the mixing tank; the chloral hydrate adding component is fixedly arranged on the top end face of the box body and is positioned right above the mixing tank; the sample discharging seat is fixedly arranged on one side, close to the sample moving mechanism, of the box body, a sample feeding port is arranged in the sample discharging seat, and the sample feeding port is located right above the sample moving mechanism.
In a possible implementation mode, a sample feeding pipe is further arranged in the material processing cavity in the box body, two ends of the sample feeding pipe are respectively connected with the bottom end of the inner cavity of the mixing tank and a sample feeding port of the sample discharging seat, a sample feeding pump is arranged in the middle of the sample feeding pipe, and the sample feeding pump is connected with the sample feeding pipe.
In one possible embodiment, the gas cleaning assembly comprises: the filter box is fixedly arranged in the gas purifying cavity and comprises a molecular sieve layer and an active carbon layer; the separator is provided with an air pressure balance channel, and under the condition that the exhaust pipe is in a negative pressure state, experimental generated gas passes through the air pressure balance channel and penetrates through the impurity filter screen, the molecular sieve layer and the active carbon layer step by step.
In a possible embodiment, the sample moving mechanism includes: the conveying motor is fixedly arranged in the middle of the top end of the base, a horizontal conveying disc is further arranged at the top end of the conveying motor, slide glass grooves are formed in two ends of the conveying disc, and heating areas and slide glass holes are formed in the slide glass grooves.
In one possible embodiment, the comminution assembly comprises: the crushing container, crushing container fixed placement is in on the top terminal surface of box, crushing container's bottom is provided with crushing motor, crushing axle in the crushing motor extends to crushing container's inner chamber bottom, crushing container's bottom still is connected with the one end of passage for in will smashing the medicine powder guide stirred tank, be provided with a solenoid valve on the other end of passage.
In a possible implementation manner, a second electromagnetic valve is further arranged on the outer wall of the box body at one end of the material processing cavity, and the first electromagnetic valve and the second electromagnetic valve are connected with the control panel.
In one possible embodiment, the chloral hydrate addition assembly comprises: the chloral hydrate adding container is fixedly arranged on the end face of the top end of the box body, a liquid outlet is arranged at the bottom end of the chloral hydrate adding container, a liquid outlet valve is arranged on the liquid outlet, and a liquid inlet is arranged at the top end of the chloral hydrate adding container.
In a second aspect, the present application provides a method of using a microscopy device for identification of a chinese drug composition, comprising the following steps:
crushing a traditional Chinese medicine sample; placing the Chinese medicine sample into a crushing container, and starting a crushing motor to effectively crush the sample by a crushing shaft;
conveying the crushed medicine powder into a stirring tank, closing a second electromagnetic valve, opening a first electromagnetic valve, and conveying the crushed medicine powder into the mixing tank through a material guide pipe by means of a negative pressure vacuum pump;
adding the chloral hydrate into the chloral hydrate adding container, and adding a proper amount of chloral hydrate into the chloral hydrate adding container through a liquid inlet;
adding chloral hydrate into a stirring tank, opening a liquid discharge valve, and enabling the chloral hydrate to flow into the stirring tank to be mixed with medicine powder through the dead weight principle;
stirring and mixing, starting a stirring device, and fully mixing the medicine powder and chloral hydrate to form a uniform mixture;
sample conveying, namely, introducing the stirred mixture into a sample moving mechanism through a discharge hole arranged at the bottom of the stirring tank for heating treatment, and then conveying the mixture into a microscopic mechanism for observation and analysis operation;
and (3) gas purification treatment, namely closing the first electromagnetic valve, opening the second electromagnetic valve, introducing generated gas into a gas purification cavity through a gas pressure balance channel, and purifying sequentially through an impurity filter screen, a molecular sieve layer and an active carbon layer.
The application provides a microscopic device for traditional chinese medicine ingredient appraisal and application method thereof is provided with structures such as base, microscopic mechanism, traditional chinese medicine processing mechanism, move appearance mechanism, lighting mechanism, the aperture, the stabilizer blade, control panel and blast pipe cooperate each other, realize automaticly, simplify the operation flow, improve appraisal efficiency and accuracy, wherein, microscopic mechanism is responsible for enlarging the observation sample, lighting mechanism provides the light source, traditional chinese medicine processing mechanism is responsible for preliminary treatment, move appearance mechanism and pass the sample between the two, aperture regulation definition, control panel centralized control whole device function, the blast pipe improves operational safety, discharge poisonous, pungent smell, traditional chinese medicine processing mechanism inside contains a plurality of subassemblies in order to realize sample pretreatment and gas purification, reduce manual operation error, improve sample processing efficiency and security, thereby effectively improve accuracy and the convenience of traditional chinese medicine ingredient appraisal.
Drawings
Fig. 1 is a schematic structural diagram of a microscopic device for identifying components of a traditional Chinese medicine and a first angle of a using method thereof according to an embodiment of the present application;
FIG. 2 is a schematic view of a second angle of a microscopic device for identifying components of traditional Chinese medicine and a method of using the same according to an embodiment of the present application;
fig. 3 is a schematic structural view of a traditional Chinese medicine treatment mechanism according to an embodiment of the present application;
FIG. 4 shows an enlarged view of the A partial structure of FIG. 2;
FIG. 5 shows a schematic structural diagram of a mixing tank provided in an embodiment of the present application;
fig. 6 shows a schematic structural diagram of a gas cleaning assembly provided in an embodiment of the present application.
In the figure: 1. base, 2, microscopy mechanism, 3, traditional chinese medicine handling mechanism, 4, sample moving mechanism, 5, lighting mechanism, 6, gel aperture, 7, stand, 8, control panel, 9, exhaust pipe, 10, sample pump, 11, sample pipe, 12, feed pipe, 13, first solenoid valve, 14, second solenoid valve, 15, air pressure balancing channel, 21, coarse focusing screw adjusting mechanism, 22, movable support, 23, display, 24, lens barrel, 25, eyepiece assembly, 31, box, 32, baffle, 33, impurity filter screen, 34, gas purifying assembly, 35, negative pressure vacuum pump, 36, pulverizing assembly, 37, mixing tank, 38, chloral hydrate adding assembly, 39, sample discharging seat, 41, conveying motor, 42, conveying tray, 43, heating assembly, 341, filter tank, 342, molecular sieve layer 353, activated carbon layer 361, pulverizing container 362, pulverizing motor 381, chloral hydrate adding container, 382, liquid inlet, 383, liquid discharge valve.
Detailed Description
In order to better understand the technical solutions provided by the embodiments of the present specification, the following detailed description of the technical solutions of the embodiments of the present specification is made through the accompanying drawings and the specific embodiments, and it should be understood that the specific features of the embodiments of the present specification are detailed descriptions of the technical solutions of the embodiments of the present specification, and not limit the technical solutions of the present specification, and the technical features of the embodiments of the present specification may be combined with each other without conflict.
In this document, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element. The term "two or more" includes two or more cases.
The microscopic device for identifying the components of the traditional Chinese medicine and the using method thereof mainly aim to solve the problems of complex operation, lower safety, misoperation and larger sample pollution risk.
Referring to fig. 1-6, a first aspect of an embodiment of the present application provides a microscopic device for identifying components of a traditional Chinese medicine and a method for using the same, including: a base 1, a microscope mechanism 2, a traditional Chinese medicine treatment mechanism 3, a sample moving mechanism 4, an illumination mechanism 5, a condenser aperture 6, a support leg 7, a control panel 8 and an exhaust pipe 9; the microscopic mechanism 2 is fixedly arranged at one side of the top end of the base 1, and a plurality of supporting feet 7 are arranged at the bottom end of the base 1; the traditional Chinese medicine treatment mechanism 3 is arranged on the other side of the top end of the base 1, a sample moving mechanism 4 is arranged between the traditional Chinese medicine treatment mechanism 3 and the microscopic mechanism 2, and an exhaust pipe 9 is arranged on the traditional Chinese medicine treatment mechanism 3; the illumination mechanism 5 is arranged in the base 1 and corresponds to the position of the micro mechanism 2; the diaphragm 6 is rotatably arranged on the microscopic mechanism 2; the control panel 8 is fixedly arranged on the traditional Chinese medicine processing mechanism 3.
According to the technical scheme, the microscopic device for identifying the traditional Chinese medicine components is characterized in that the structures such as the base 1, the microscopic mechanism 2, the traditional Chinese medicine treatment mechanism 3, the sample moving mechanism 4, the illumination mechanism 5, the aperture 6, the support legs 7, the control panel 8 and the exhaust pipe 9 are arranged, so that automation is realized, the operation flow is simplified, the identification efficiency and accuracy are improved, and in the using process, the microscopic mechanism 2 is responsible for amplifying and observing a sample, and the illumination mechanism 5 corresponding to the microscopic mechanism is used for providing a sufficient light source for observation; the traditional Chinese medicine treatment mechanism 3 is mainly responsible for pretreatment (such as crushing, mixing and the like) of samples, errors caused by manual operation are further reduced, the sample transferring mechanism 4 plays a role in transferring the samples between the traditional Chinese medicine treatment mechanism 3 and the sample pretreated by the traditional Chinese medicine treatment mechanism 3, the microscope mechanism 2 is moved to observe, the aperture 6 can adjust the definition of microscopic observation, the control panel 8 centrally controls the operation of the whole device, meanwhile, the exhaust pipe 9 is connected to the traditional Chinese medicine treatment mechanism 3, toxic and pungent smell is effectively discharged, the operation safety is improved, and through the mutual cooperation of the structures, the automation of the identification process of traditional Chinese medicine components is realized, the operation flow is simplified, the identification efficiency and accuracy are improved, and the risks of misoperation and sample pollution are reduced.
Referring to fig. 1-2, in some examples, the microscopy mechanism 2 further includes: a coarse focusing screw adjusting mechanism 21, a movable bracket 22, a display 23, a lens cone 24 and an eyepiece assembly 25; the coarse focusing spiral adjusting mechanism 21 is arranged at one side of the top end of the base 1, and a telescopic movable bracket 22 is arranged on the coarse focusing spiral adjusting mechanism 21; the display 23 is fixedly arranged on the front side of the movable bracket 22, a lens barrel 24 is also arranged below the display 23, a rotatable switching eyepiece assembly 25 is arranged at the bottom end of the lens barrel 24, and the lens barrel 24 corresponds to the position of the illumination mechanism 5.
It can be understood that the microscope mechanism 2 comprises a coarse focusing spiral adjusting mechanism 21, a movable bracket 22, a display 23, a lens cone 24 and an eyepiece assembly 25, which jointly realize high-definition observation of the traditional Chinese medicine sample, wherein the coarse focusing spiral adjusting mechanism 21 is positioned at one side of the top end of the base 1, and can quickly adjust the focal length; the movable support 22 is connected with the coarse focusing spiral adjusting mechanism 21, the scalability enables the microscopic mechanism 2 to have higher flexibility, the display 23 is fixed on the front side of the movable support 22, a user can observe the amplified sample image directly conveniently, the lens barrel 24 is located below the display 23 and corresponds to the position of the illuminating mechanism 5, the sample is ensured to be fully illuminated, the bottom end of the lens barrel 24 is provided with the rotatable switching eyepiece assembly 25, the switching observation among different multiples is facilitated, the whole microscopic mechanism 2 realizes clear and efficient observation of the sample in the identification process of the traditional Chinese medicine components through the synergistic effect of the parts, and the accuracy and convenience of identification are improved.
Referring to fig. 3-6, in some examples, the traditional Chinese medicine processing mechanism 3 further includes: the device comprises a box body 31, a partition plate 32, an impurity filter screen 33, a gas purifying component 34, a negative pressure vacuum pump 35, a crushing component 36, a mixing tank 37, a chloral hydrate adding component 38 and a sample discharging seat 39, wherein the box body 31 is fixedly arranged at one side of the top end of the base 1, which is far away from the microscopic mechanism 2, the box body 31 is in a cavity state, and an exhaust pipe 9 is arranged on the outer wall of the box body 31; the partition plate 32 is fixedly arranged in the inner cavity of the box body 31, and divides the box body 31 into a material processing cavity and a gas purifying cavity; the impurity filter screen 33 is fixedly arranged in the gas purifying cavity, and a gas purifying component 34 is arranged on one side, close to the exhaust pipe 9, of the impurity filter screen 33; the negative pressure vacuum pump 35 is fixedly arranged at the top end of the box body 31 and is connected with the exhaust pipe 9; the crushing assembly 36 is fixedly arranged on the end face of the top end of the box body 31 of the material processing cavity, and the mixing tank 37 is fixedly arranged at the bottom end of the material processing cavity far away from one end of the crushing assembly 36; the mixing tank 37 is internally provided with a magnetic stirrer, and the chloral hydrate adding component 38 is fixedly arranged on the top end surface of the box body 31 and is positioned right above the mixing tank 37; the sample discharging seat 39 is fixedly arranged at one side of the box body 31 close to the sample moving mechanism 4, a sample feeding port is arranged in the sample discharging seat 39, and the sample feeding port is positioned right above the sample moving mechanism 4.
It will be appreciated that the traditional Chinese medicine treatment mechanism 3 is composed of a plurality of components to realize pretreatment and gas purification of samples, the box 31 is arranged at the top end of the base 1, and the inner part is a material treatment cavity and a gas purification cavity; the crushing assembly 36 is positioned at the top end of the material processing cavity, convenient for observation and material input, the mixing tank 37 is arranged at the bottom end of the material processing cavity and used for sample mixing, the chloral hydrate adding assembly 38 is positioned right above the mixing tank 37, chloral hydrate can be added into the mixing tank 37, the sample discharging seat 39 is arranged at one side close to the sample moving mechanism 4 and is provided with a sample feeding port, so that a processed sample is convenient to be conveyed to the sample moving mechanism 4 and is moved to the microscopic mechanism 2 for observation through the sample moving mechanism 4, the impurity filter screen 33 and the gas purifying assembly 34 are arranged in the gas purifying cavity and are responsible for removing generated toxic or irritant gas, the negative pressure vacuum pump 35 is connected with the exhaust pipe 9, thus the negative pressure environment in the box 31 is kept, airflow is driven to flow, the operation safety is improved, and the harmful gas diffusion generated by chloral hydrate is prevented, therefore, the manual operation error is reduced, the sample processing efficiency and the safety are improved, and the accuracy and convenience of the identification of Chinese medicine components are effectively improved through the cooperation of all components of the whole Chinese medicine processing mechanism 3.
Referring to fig. 5-6, in some examples, further, a sample tube 11 is further disposed in the material processing chamber in the box 31, two ends of the sample tube 11 are respectively connected to the bottom end of the inner cavity of the mixing tank 37 and the sample inlet of the sample discharging seat 39, a sample pump 10 is disposed in the middle of the sample tube 11, and the sample pump 10 is connected to the sample tube 11.
In this example, the sample tube 11 is located in the material processing chamber and connects the bottom end of the mixing tank 37 to the sample supply port of the sample discharge seat 39. Its main function is to effect transfer of the sample from the mixing tank 37 to the sample holder 39 during operation. The sample injection pump 10 is connected with the sample injection pipe 11 and is responsible for controlling the flow of the sample in the sample injection pipe 11. In the pretreatment stage, the sample after the steps of pulverization, mixing, etc. is sent to the mixing tank 37; the sample is then driven along the sample tube 11 by the sample pump 10 and finally transported to the sample supply port of the sample ejection seat 39. At this time, the sample transfer mechanism 4 can conveniently take out the sample from the sample supply port and transfer the sample to the microscope mechanism 2 for observation. The whole structure simplifies the sample treatment and transmission process, improves the efficiency of identifying the components of the traditional Chinese medicine, and reduces the risks of manual operation errors and sample pollution through automatic operation.
Referring to fig. 3 and 6, in some examples, further, the gas cleaning assembly 34 includes: the filter box 341, the filter box 341 is fixedly arranged in the gas purifying cavity, and the filter box 341 comprises a molecular sieve layer 342 and an active carbon layer 353.
It will be appreciated that the main function of the gas cleaning module 34 is to remove toxic or irritant gases generated during the process, and to ensure safe operation, which includes a filter box 341, a built-in molecular sieve layer 342 and an activated carbon layer 353, when the gases enter the gas cleaning chamber, the gases first pass through the molecular sieve layer 342, the molecular sieve can adsorb toxic or irritant molecules of a specific size and collect the same from the gases, then the gases continue to flow through the activated carbon layer 353, the activated carbon has a large surface area and strong adsorption capacity, the residual toxic or irritant substances can be adsorbed and removed, the gases filtered by the two layers are cleaned, and then discharged through the exhaust pipe 9, so that the risk of toxic or irritant gases during the process is effectively reduced, the operation safety is improved, and the identification process of the components of the traditional Chinese medicine is safer and more reliable.
Referring to fig. 3, in some examples, a gas pressure balancing channel 15 is further provided on the partition 32, and in a negative pressure state of the exhaust pipe 9, the experiment generated gas passes through the gas pressure balancing channel 15 and penetrates the impurity filter 33, the molecular sieve layer 342 and the activated carbon layer 353 step by step.
It can be understood that the air pressure balancing channel 15 is arranged on the partition board 32, and is used for keeping air pressure balance between the material processing cavity and the air purifying cavity, when the exhaust pipe 9 is in a negative pressure state, air generated in an experiment enters the air purifying cavity through the air pressure balancing channel 15, and the air passes through the impurity filter screen 33, the molecular sieve layer 342 and the active carbon layer 353 in sequence, so that step-by-step filtration and purification are realized, and the impurity filter screen 33 intercepts larger particle impurities; molecular sieve layer 342 adsorbs toxic or irritant molecules of a particular size; the activated carbon layer 353 removes the remaining toxic or irritating substances and the arrangement of the gas pressure equalization channel 15 enables the gas to flow effectively from the material processing chamber to the gas cleaning chamber, improving operational safety, optimizing gas flow paths and enhancing gas cleaning.
Referring to fig. 1, 2 and 4, in some examples, the sample moving mechanism 4 further includes: the conveying motor 41, the conveying motor 41 is fixedly arranged in the middle of the top end of the base 1, a horizontal conveying disc 42 is further arranged at the top end of the conveying motor 41, slide grooves are formed in two ends of the conveying disc 42, a heating area and a slide hole are formed in the slide grooves, and a heating assembly 43 is arranged in the heating area.
It will be appreciated that the sample moving mechanism 4 is mainly responsible for transferring the stirred mixture onto a glass slide for experimental operation, the conveying motor 41 is fixed in the middle of the top end of the base 1, the top end is provided with a horizontal conveying disc 42, two ends of the conveying disc 42 are provided with glass slide grooves, which comprise a heating zone and glass slide holes, a heating device is arranged in the heating zone for adjusting the temperature, the mixture can realize accurate temperature control in the transferring process, the stability and the reliability of experimental operation are improved, meanwhile, the operation flow is simplified, and the working efficiency is improved.
It is noted that, mixing chloral hydrate with traditional chinese medicine powder and making microscopic observation sample intensifies, helps improving traditional chinese medicine powder's observation effect, compares with other solvents, and chloral hydrate can better show traditional chinese medicine powder's characteristic structure, and chloral hydrate has certain disinfection effect of disinfecting, can restrain or kill the microorganism that probably exists in microscopic observation process, improves experimental result's accuracy, and mixed sample is difficult to take place rotten, degradation or reaction in microscopic observation process, is favorable to obtaining stable, reliable experimental data.
Referring to fig. 3, 5 and 6, in some examples, the comminution assembly 36 further comprises: the crushing container 361, the crushing container 361 is fixedly arranged on the top end face of the box body 31, the bottom end of the crushing container 361 is provided with a crushing motor 362, a crushing shaft in the crushing motor 362 extends to the bottom end of the inner cavity of the crushing container 361, the bottom end of the crushing container 361 is also connected with one end of a material guiding pipe 12 for guiding crushed medicine powder into the stirring tank, and the other end of the material guiding pipe 12 is provided with a first electromagnetic valve 13.
It will be appreciated that the core of the shredder assembly 36 is a shredder receptacle 361 which is located at the top of the bin 31 and secured to the end surface. A pulverizing motor 362 is installed in the pulverizing container 361, and the pulverizing shaft is driven to pulverize the Chinese medicine sample. The crushed Chinese medicine powder is conveyed into a stirring tank through a material guide pipe 12 connected with the bottom. The operation of the whole crushing assembly 36 realizes the efficient crushing and transmission of the traditional Chinese medicine samples, improves the pretreatment efficiency, reduces the manual operation error and the sample pollution risk, and is beneficial to improving the accuracy and convenience of the identification of the traditional Chinese medicine components.
Referring to fig. 2 and 4, in some examples, a second electromagnetic valve 14 is further disposed on an outer wall of the box 31 at one end of the material processing chamber, and the first electromagnetic valve 13 and the second electromagnetic valve 14 are connected to the control panel 8.
It should be noted that, the second electromagnetic valve 14 is located on the outer wall of the box 31 at one end of the material processing cavity, and is connected to the control panel 8 together with the first electromagnetic valve 13, the negative pressure vacuum pump 35 plays a key role in this system, and is used for guiding and controlling the transportation of gas and materials, when the gas purification process is performed, the second electromagnetic valve 14 is opened, the first electromagnetic valve 13 is closed, so that the effective flow of the air flow can be realized, the gas purification effect is ensured, when the materials in the crushing assembly 36 need to be conveyed to the mixing tank 37, the second electromagnetic valve 14 is closed, the first electromagnetic valve 13 is opened, the crushed powder is guided into the mixing tank 37 by means of the negative pressure generated by the negative pressure vacuum pump 35, the whole structure utilizes the two electromagnetic valves and the negative pressure vacuum pump 35 to mutually cooperate, so that the smooth switching between the gas purification and the material conveying is realized, the operation efficiency is improved, the risk of misoperation is reduced, and the safety and accuracy of the processing process are ensured.
Referring to fig. 1, 2, 3, 5 and 6, in some examples, the chloral hydrate addition assembly 38 further includes: the chloral hydrate adding container 381 is fixedly arranged on the top end face of the box body 31, a liquid outlet is arranged at the bottom end of the chloral hydrate adding container 381, a liquid outlet valve 383 is arranged on the liquid outlet, and a liquid inlet 382 is arranged at the top end of the chloral hydrate adding container 381.
In this example, the chloral hydrate addition assembly 38 is primarily used to add an appropriate amount of chloral hydrate to the agitation tank. The assembly comprises a chloral hydrate addition container 381 fixed at the top of the box 31, and a liquid outlet valve 383 are arranged at the bottom for controlling the release of chloral hydrate. The top end is provided with a liquid inlet 382 for adding and replenishing chloral hydrate into the container. In the actual operation, the chloral hydrate stored in the chloral hydrate addition container 381 is flowed into the agitation tank by opening the drain valve 383 by using the principle of self weight, and mixed with other materials. The structure simplifies the adding process of chloral hydrate, improves the operation convenience and accuracy, and reduces the risk of contacting toxic substances by operators.
In a second aspect, the present application provides a method for using a microscopic device for identifying components of a traditional Chinese medicine, including the following steps:
crushing a traditional Chinese medicine sample; placing the Chinese medicine sample into a crushing container 361, and starting a crushing motor 362 to effectively crush the sample by a crushing shaft; conveying the crushed medicine powder into a stirring tank, closing the second electromagnetic valve 14, opening the first electromagnetic valve 13, and conveying the crushed medicine powder into a mixing tank 37 through a material guide pipe 12 by means of a negative pressure vacuum pump 35; adding chloral hydrate into the chloral hydrate adding container 381, and adding a proper amount of chloral hydrate into the chloral hydrate adding container 381 through a liquid inlet 382; adding chloral hydrate into a stirring tank, opening a drain valve 383, and enabling the chloral hydrate to flow into the stirring tank to be mixed with medicine powder by the self-weight principle; stirring and mixing, starting a stirring device, and fully mixing the medicine powder and chloral hydrate to form a uniform mixture; sample conveying, namely, introducing the stirred mixture into a sample moving mechanism 4 through a discharge hole arranged at the bottom of the stirring tank for heating treatment, and then conveying the mixture into a microscopic mechanism 2 for observation and analysis operation; the gas purifying treatment, closing the first electromagnetic valve 13, opening the second electromagnetic valve 14, introducing the generated gas into the gas purifying cavity through the gas pressure balancing channel 15, and purifying sequentially through the impurity filter screen 33, the molecular sieve layer 342 and the activated carbon layer 353.
It will be appreciated by those skilled in the art that embodiments of the present application may be provided as a method, system, or computer program product. Accordingly, the present application may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present application may take the form of a computer program product embodied on one or more computer-usable computer-readable storage media (including, but not limited to, disk storage, CD-ROM, optical storage, etc.) having computer-usable program code embodied therein.
The foregoing is merely exemplary of the present application and is not intended to limit the present application. Various modifications and changes may be made to the present application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc. which are within the spirit and principles of the present application are intended to be included within the scope of the claims of the present application.
Claims (10)
1. A microscopic device for identification of components of a chinese medicine, comprising:
the micro-mechanism is fixedly arranged at one side of the top end of the base, and a plurality of supporting feet are arranged at the bottom end of the base;
the traditional Chinese medicine treatment mechanism is arranged on the other side of the top end of the base, a sample moving mechanism is arranged between the traditional Chinese medicine treatment mechanism and the microscopic mechanism, and an exhaust pipe is arranged on the traditional Chinese medicine treatment mechanism;
an illumination mechanism disposed in the base and corresponding to a position of the microscopy mechanism;
the condenser ring is rotatably arranged on the microscopic mechanism;
the control panel is fixedly arranged on the traditional Chinese medicine treatment mechanism.
2. The microscopic device for identification of components of chinese medicine according to claim 1, wherein: the microscopy mechanism comprises:
the coarse focusing spiral adjusting mechanism is arranged at one side of the top end of the base, and a telescopic movable bracket is arranged on the coarse focusing spiral adjusting mechanism;
the display is fixedly arranged on the front side of the movable support, a lens barrel is further arranged below the display, a rotatable switching eyepiece assembly is arranged at the bottom end of the lens barrel, and the lens barrel corresponds to the illumination mechanism in position.
3. The microscopic device for identification of components of chinese medicine according to claim 1, wherein: the traditional Chinese medicine treatment mechanism comprises:
the box body is fixedly arranged at one side, far away from the microscopic mechanism, of the top end of the base, the box body is in a cavity state, and an exhaust pipe is arranged on the outer wall of the box body;
the partition board is fixedly arranged in the inner cavity of the box body and divides the box body into a material processing cavity and a gas purifying cavity;
the impurity filter screen and the gas purification assembly are fixedly arranged in the gas purification cavity, and the gas purification assembly is further arranged on one side, close to the exhaust pipe, of the impurity filter screen;
the negative pressure vacuum pump is fixedly arranged at the top end of the box body and is connected with the exhaust pipe;
the crushing assembly is fixedly arranged on the end face of the top end of the box body of the material processing cavity, the mixing tank is fixedly arranged at the bottom end of the material processing cavity far away from one end of the crushing assembly, and a magnetic stirrer is arranged in the mixing tank;
the chloral hydrate adding component is fixedly arranged on the top end face of the box body and is positioned right above the mixing tank;
the sample discharging seat is fixedly arranged on one side, close to the sample moving mechanism, of the box body, a sample feeding port is arranged in the sample discharging seat, and the sample feeding port is located right above the sample moving mechanism.
4. A microscopic device for identification of components of chinese medicine according to claim 3, wherein:
the material treatment cavity in the box body is also provided with a sample feeding pipe, two ends of the sample feeding pipe are respectively connected with the bottom end of the inner cavity of the mixing tank and a sample feeding port of the sample discharging seat, the middle part of the sample feeding pipe is provided with a sample feeding pump, and the sample feeding pump is connected with the sample feeding pipe.
5. A microscopic device for identification of components of chinese medicine according to claim 3, wherein: the gas cleaning assembly includes:
the filter box is fixedly arranged in the gas purifying cavity and comprises a molecular sieve layer and an active carbon layer;
the separator is provided with an air pressure balance channel, and under the condition that the exhaust pipe is in a negative pressure state, experimental generated gas passes through the air pressure balance channel and penetrates through the impurity filter screen, the molecular sieve layer and the active carbon layer step by step.
6. A microscopic device for identification of components of chinese medicine according to claim 3, wherein: the sample moving mechanism comprises:
the conveying motor is fixedly arranged in the middle of the top end of the base, a horizontal conveying disc is further arranged at the top end of the conveying motor, slide glass grooves are formed in two ends of the conveying disc, and heating areas and slide glass holes are formed in the slide glass grooves.
7. A microscopic device for identification of components of chinese medicine according to claim 3, wherein: the comminution assembly includes:
the crushing container, crushing container fixed placement is in on the top terminal surface of box, crushing container's bottom is provided with crushing motor, crushing axle in the crushing motor extends to crushing container's inner chamber bottom, crushing container's bottom still is connected with the one end of passage for in will smashing the medicine powder guide stirred tank, be provided with a solenoid valve on the other end of passage.
8. The microscopic device for identification of components of chinese medicine according to claim 7, wherein:
the outer wall of the box body at one end of the material processing cavity is also provided with a second electromagnetic valve, and the first electromagnetic valve and the second electromagnetic valve are connected with the control panel.
9. A microscopic device for identification of components of chinese medicine according to claim 3, wherein: the chloral hydrate addition assembly comprises:
the chloral hydrate adding container is fixedly arranged on the end face of the top end of the box body, a liquid outlet is arranged at the bottom end of the chloral hydrate adding container, a liquid outlet valve is arranged on the liquid outlet, and a liquid inlet is arranged at the top end of the chloral hydrate adding container.
10. A method of using a microscopic device for identification of components of chinese medicine according to any one of claims 1-9, comprising the following steps:
crushing a traditional Chinese medicine sample; placing the Chinese medicine sample into a crushing container, and starting a crushing motor to effectively crush the sample by a crushing shaft;
conveying the crushed medicine powder into a stirring tank, closing a second electromagnetic valve, opening a first electromagnetic valve, and conveying the crushed medicine powder into the mixing tank through a material guide pipe by means of a negative pressure vacuum pump;
adding the chloral hydrate into the chloral hydrate adding container, and adding a proper amount of chloral hydrate into the chloral hydrate adding container through a liquid inlet;
adding chloral hydrate into a stirring tank, opening a liquid discharge valve, and enabling the chloral hydrate to flow into the stirring tank to be mixed with medicine powder through the dead weight principle;
stirring and mixing, starting a stirring device, and fully mixing the medicine powder and chloral hydrate to form a uniform mixture;
sample conveying, namely, introducing the stirred mixture into a sample moving mechanism through a discharge hole arranged at the bottom of the stirring tank for heating treatment, and then conveying the mixture into a microscopic mechanism for observation and analysis operation;
and (3) gas purification treatment, namely closing the first electromagnetic valve, opening the second electromagnetic valve, introducing generated gas into a gas purification cavity through a gas pressure balance channel, and purifying sequentially through an impurity filter screen, a molecular sieve layer and an active carbon layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310683870.2A CN116519692A (en) | 2023-06-10 | 2023-06-10 | Microscopic device for identifying traditional Chinese medicine components and application method thereof |
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| CN202310683870.2A CN116519692A (en) | 2023-06-10 | 2023-06-10 | Microscopic device for identifying traditional Chinese medicine components and application method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117491358A (en) * | 2024-01-03 | 2024-02-02 | 四川省中医药科学院 | Device and method for identifying traditional Chinese medicinal materials based on microscopic observation |
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- 2023-06-10 CN CN202310683870.2A patent/CN116519692A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117491358A (en) * | 2024-01-03 | 2024-02-02 | 四川省中医药科学院 | Device and method for identifying traditional Chinese medicinal materials based on microscopic observation |
| CN117491358B (en) * | 2024-01-03 | 2024-03-22 | 四川省中医药科学院 | Device and method for identifying traditional Chinese medicinal materials based on microscopic observation |
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