CN116519139A - Raman spectrum measuring method and device - Google Patents

Abstract

The embodiment of the application provides a Raman spectrum measurement method, which is applied to Raman spectrum measurement equipment, wherein the Raman spectrum measurement equipment comprises a photon output unit, a detection unit, a delay unit and a photon collection unit, and the Raman spectrum measurement method comprises the following steps: outputting a pulse optical signal through the photon output unit, wherein the pulse optical signal is used for irradiating a medium to be detected; monitoring the number of photons within a preset range around the photon collection unit through the detection unit; controlling the delay of the photon collecting unit through the delay unit, wherein the delay time is determined according to the photon quantity; wherein the end time of the delay is later than the end time of the pulsed light signal; and under the condition that the time delay is over, starting the photon collecting unit to collect photons scattered by the medium to be tested. According to the technical scheme, interference and noise in the measuring process can be filtered, so that accuracy and stability of a measuring result are improved.

Description

Raman spectrum measuring method and device

Technical Field

The application relates to the technical field of optical measurement, in particular to a Raman spectrum measurement method.

Background

With the development of biomedical inspection techniques, raman spectroscopy is increasingly used in biochemical analysis. The Raman spectrum is a scattering spectrum generated by energy exchange between photons and molecules to be detected after inelastic collision, and can reflect characteristic information of the molecules to be detected. Thus, the Raman spectrum can be used for analyzing various components in the body fluid, and the measuring process does not need to puncture the skin of a patient, so that the patient is not painful. And the measuring speed of the Raman spectrum is faster than that of the traditional biochemical analysis, so that the analysis result can be obtained more quickly, and the Raman spectrum has great advantages in the fields of first aid, operation and the like.

However, in practical application of raman spectroscopy measurement, noise and interference in various measurement processes may bring about a lot of uncertainty to the measurement result, thereby reducing the accuracy of biochemical analysis.

It should be noted that the foregoing is not necessarily prior art, and is not intended to limit the scope of the patent protection of the present application.

Disclosure of Invention

Embodiments of the present application provide a raman spectroscopy measurement method to solve or alleviate one or more of the technical problems set forth above.

As an aspect of the embodiments of the present application, the embodiments of the present application provide a raman spectrum measurement method applied to a raman spectrum measurement apparatus including a photon output unit, a detection unit, a delay unit, and a photon collection unit, the raman spectrum measurement method including:

outputting a pulse optical signal through the photon output unit, wherein the pulse optical signal is used for irradiating a medium to be detected; the pulse optical signals sequentially pass through a first medium, a second medium and the medium to be detected, and scatter in different mediums to respectively form a first medium scattered photon, a second medium scattered photon and a medium to be detected scattered photon;

monitoring the number of photons within a preset range around the photon collection unit through the detection unit;

controlling the delay of the photon collecting unit through the delay unit, wherein the delay time is determined according to the photon quantity; wherein the end time of the delay is later than the end time of the pulsed light signal;

and under the condition that the time delay is over, starting the photon collecting unit to collect photons scattered by the medium to be tested.

Optionally, the raman spectroscopy method further comprises:

controlling a time window after the photon collecting unit delays through the delay unit;

and controlling the photon collecting unit to collect scattered photons of the medium to be measured, which accords with the expected target, by adjusting the time length of the time window.

Optionally, the raman spectroscopy method further comprises:

repeating the following operations until the scattered photons of the medium to be tested meeting the preset quantity are obtained:

outputting the pulse optical signal to the medium to be tested;

and under the control of the delay unit, collecting scattered photons of the medium to be detected, which are generated when the pulse light signal reaches the medium to be detected, through the photon collecting unit.

Optionally, the raman spectrum measurement method further comprises:

controlling the photon collecting unit to collect scattered photons of a target medium to be measured by adjusting the end time of the delay; the scattered photons of the target medium to be measured are generated when the pulse optical signal reaches the appointed depth in the medium to be measured.

Optionally, the raman spectrum measurement apparatus further includes a light source control unit, and the raman spectrum measurement method includes:

the light source control unit sends pulse signals to the photon output unit to control the working time of the photon output unit;

wherein the operating time is the duration of the pulse signal.

Optionally, the medium to be tested comprises subcutaneous body fluid of a specified depth.

Another aspect of an embodiment of the present application provides a raman spectroscopy apparatus comprising:

the photon output unit is used for outputting a pulse optical signal; the pulse optical signal is used for irradiating the medium to be measured through the intermediate medium;

a photon collection unit for collecting photons;

the detection unit is used for monitoring the photon quantity in a preset range around the photon collection unit;

a delay unit for delaying a time window in which the photon collection unit collects photons; wherein a delay time is dependent on the number of photons and an end time of the delay is later than an end time of the pulsed light signal;

the delayed time window corresponds to the time when the scattered photons of the medium to be detected reach the photon collecting unit, and the scattered photons of the medium to be detected are generated when the pulse light signals reach the medium to be detected.

Optionally, the raman spectrum measurement apparatus further comprises:

the light source control unit is used for controlling the working time of the photon output unit through a pulse signal;

wherein the operating time is the duration of the pulsed light signal.

Optionally, the delay unit is further configured to:

and adjusting the time length of the time window so as to control the photon collecting unit to collect scattered photons of the medium to be measured, which accords with the expected target.

Optionally, the delay unit is further configured to:

adjusting the ending time of the delay, and controlling the photon collecting unit to collect scattered photons of a target medium to be measured;

the scattered photons of the target medium to be measured are generated when the pulse optical signal reaches the appointed depth in the medium to be measured.

The technical scheme adopted by the embodiment of the application can comprise the following advantages:

and the photon output unit transmits a pulse optical signal, and the pulse optical signal reaches the medium to be measured through the multi-layer intermediate medium. Wherein photons are scattered in different medium layers to form intermediate medium scattered photons and medium scattered photons to be measured respectively. In the process, the photon quantity near the photon collecting unit is monitored in real time through the detecting unit, and the delay of the photon collecting unit is controlled through the delay unit. When the photon quantity is reduced to a certain threshold value, the time delay is ended and the photon collecting unit is started, so that the photon collecting unit mainly collects photons of the medium to be measured. According to the embodiment of the application, the delay unit is used for controlling the delay starting of the photon collecting unit, and as scattered photons of the intermediate medium basically disappear in the delay time, the photon collecting unit mainly collects scattered photons of the medium to be measured, interference and noise in the measuring process can be filtered, and therefore accuracy and stability of a measuring result are improved.

Drawings

In the drawings, the same reference numerals refer to the same or similar parts or elements throughout the several views unless otherwise specified. The figures are not necessarily drawn to scale. It is appreciated that these drawings depict only some embodiments according to the disclosure and are not therefore to be considered limiting of its scope.

FIG. 1 is a block diagram of a Raman spectrum measurement apparatus provided by an embodiment of the present application;

FIG. 2 schematically illustrates a flow chart of a Raman spectroscopy method according to a first embodiment of the present application;

fig. 3 schematically shows an added flow of the raman spectrum measurement method according to the first embodiment of the present application;

fig. 4 schematically shows an added flow of the raman spectrum measurement method according to the first embodiment of the present application;

fig. 5 schematically shows an added flow of the raman spectrum measurement method according to the first embodiment of the present application;

fig. 6A to 6B are schematic diagrams illustrating an application example of the raman spectrum measurement method according to the first embodiment of the present application.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present application will be further described in detail with reference to the accompanying drawings and examples. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other. The present application will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

It should be noted that the terms "first," "second," and the like in the description and claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the terms so used are interchangeable under appropriate circumstances such that the embodiments of the application described herein are, for example, capable of operation in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

The following provides a term explanation of the present application.

Scattering of light: refers to the phenomenon in which photons travel off the original direction as light passes through an inhomogeneous medium. When the light irradiates the surface of an object, two types of scattering are formed, namely, rayleigh scattering and Raman scattering. Wherein, most photons (about 99.999%) are rayleigh scattered, and only few photons accounting for one ten thousand of the photons are raman scattered.

Raman scattering: a phenomenon of inelastic scattering of photons. Inelastic collision occurs between photons and molecules/atoms of the medium, energy exchange occurs, and the wavelength, frequency and energy of scattered photons change.

Rayleigh scattering: an elastic scattering phenomenon of photons. Elastic collision is sent between the photons and medium molecules/atoms, the energy before and after collision is not changed, and the wavelength, frequency and energy of the scattered photons are not changed.

Raman spectroscopy: a scattering spectrum generated based on the interaction of light and molecules of a material can be applied to molecular analysis. The Raman spectrum is analyzed to obtain information such as molecular vibration, molecular structure and the like.

Optical path: refers to the path of light propagation, including refraction in light propagation, and the path after reflection.

Transmittance: the ratio of the transmitted light flux to the incident light flux is used to indicate the degree of light transmission of the transparent body, i.e. to characterize the light transmission properties of the object. Transmission is the exit phenomenon of incident light after it has been refracted through an object. The object to be transmitted is a transparent or translucent body, such as glass, color filters, etc. If the transparent body is colorless, most of the light is transmitted through the object, except for a small amount of light that is reflected.

For the convenience of understanding the technical solutions provided in the embodiments of the present application by those skilled in the art, the following description will explain related technologies:

the raman spectrum is a scattering spectrum generated after photons and molecules are in inelastic collision, and can reflect characteristic information of the molecules. Therefore, raman spectroscopy can be applied in biochemical analysis, such as analyzing various components in a body fluid of a human body using raman spectroscopy. The advantages of raman spectroscopy are: (1) The skin of the patient is not required to be pierced, and the pain feeling is not brought to the patient. (2) The measuring speed is high, and the measuring result can be obtained within one minute. (3) Raman spectroscopy can obtain analysis results faster than conventional biochemical assays. Therefore, raman spectroscopy has great advantages in the fields of emergency treatment, surgery and the like, and even real-time analysis can be realized.

However, the applicant has appreciated that: when raman spectrum analysis is actually applied, various noises and interferences are generated in the raman spectrum measurement process, and the noises and the interferences bring a lot of uncertainties to the measurement result, so that the signal-to-noise ratio of the raman spectrum measurement result is low, thereby affecting the accuracy of biochemical analysis.

Therefore, the embodiment of the application provides a technical scheme for Raman spectrum measurement. In the technical scheme, (1) various interference amounts in the measuring process can be filtered, signals actually required to be measured are separated, and accuracy and stability of a measuring result are improved. (2) The Raman spectrum of the subcutaneous body fluid layers with different depths can be measured, and biochemical analysis can be conveniently carried out on the subcutaneous body fluid layers with different depths. See in particular below.

Hereinafter, exemplary embodiments according to the present application will be described in more detail with reference to the accompanying drawings. It should be understood that these exemplary embodiments may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

The Raman spectrum measuring method provided by the embodiment of the application can be applied to Yu Laman spectrum measuring equipment.

As shown in fig. 1, the raman spectrum measurement apparatus 100 may include a general control unit 1000, a light source control unit 1100, a photon output unit 1110, a detection unit 1200, a delay unit 1300, and a photon collection unit 1310. Wherein:

the general control unit 1000 may be electrically connected to the light source control unit 1100, the photon output unit 1110, the detection unit 1200, the delay unit 1300, and the photon collection unit 1310, and configured to transmit various control signals to different units to control the activation and deactivation of the different units.

A light source control unit 1100 for controlling the switching of the photon output unit.

And a photon output unit 1110 for outputting an optical signal. The photon output unit may comprise a laser or the like for generating a light beam.

The detecting unit 1200 is configured to detect a number of photons near the photon collecting unit.

A delay unit 1300 for controlling the photon collection unit to start in delay.

Photon collection unit 1310 for collecting photons.

The raman spectrum measuring device described in the embodiments of the present application may have various applications, for example, obtaining subcutaneous body fluid information of a human body or the like.

The technical scheme of the application is described by a plurality of embodiments by taking a Raman spectrum measuring device as an execution main body. It should be understood that these embodiments may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example 1

Fig. 2 schematically shows a flow chart of a raman spectrometry method according to a first embodiment of the present application.

As shown in fig. 2, the raman spectrometry method may include:

step S200: outputting a pulse optical signal through the photon output unit, wherein the pulse optical signal is used for irradiating a medium to be detected; the pulse optical signals sequentially pass through the first medium, the second medium and the medium to be detected, and scatter in different mediums to respectively form first medium scattered photons, second medium scattered photons and medium scattered photons to be detected.

Step S202: and monitoring the photon quantity in a preset range around the photon collecting unit through the detecting unit.

Step S204: controlling the delay of the photon collecting unit through the delay unit, wherein the delay time is determined according to the photon quantity; wherein the end time of the delay is later than the end time of the pulsed light signal.

Step S206: and under the condition that the time delay is over, starting the photon collecting unit to collect photons scattered by the medium to be tested.

In this embodiment, the photon output unit may output a pulse optical signal, where the pulse optical signal is used to irradiate the medium to be measured. In an exemplary application, the pulsed optical signal inevitably needs to pass through multiple layers of intermediate media to reach the medium under test, as it is necessary to measure subcutaneous body fluid. Therefore, the pulse light signal after being emitted sequentially passes through the first medium (air), the second medium (skin surface layer) and then reaches the medium to be measured (subcutaneous body fluid). The specific process is exemplified as follows:

(1) Some photons are scattered and reflected by dust in the air, forming first medium scattered photons. The first scattered photons reach the photon collection unit after several times of scattering and reflection.

(2) Some of the photons reach the skin surface to scatter and reflect, forming second medium scattered photons. The photons scattered by the second medium are scattered and reflected several times before reaching the photon collection unit.

(3) Some photons penetrate the skin surface layer and go deep under the skin, and when encountering various subcutaneous components (such as body fluid and tissue fluid), the photons are scattered and reflected, so that scattered photons of the medium to be detected are formed. And the scattered photons of the medium to be measured finally reach the photon collecting unit after being scattered and reflected for a plurality of times.

In this process, the skin surface acts as the primary source of reflection due to the barrier effect of the skin. Most photons scatter and reflect when they reach the skin surface. However, the skin layers of different humans can vary widely for a variety of reasons, including: (1) Natural skin tone. (2) age, number of dead skin, etc. (3) the growth environment causes the skin surface layer to be different. These differences in the skin surface can result in different reflection characteristics of the skin surface. Therefore, when raman spectroscopy is performed, subcutaneous tissue fluid/body fluid information actually required to be acquired is severely disturbed due to the difference of the characteristic information of the skin surface layer, so that the measurement result has very large uncertainty.

In this embodiment, in order to obtain real and accurate subcutaneous tissue fluid information, the number of photons in a preset range around the photon collecting unit can be monitored in real time through the detecting unit, and the photon collecting unit is controlled to start in a delayed mode through the delay unit. Wherein the delay time can be determined according to the change of the number of photons, and the end time of the delay is later than the end time of the pulsed light signal. For example, when the number of photons falls to a preset threshold, the delay may be ended and the photon collection unit activated. Since the first medium scattered photons and the second medium scattered photons have substantially disappeared within the delay time, the photons are collected by the photon collection unit after activation, primarily subcutaneous body fluid scattered photons.

In the embodiment, the delay time can be determined by monitoring the change of the photon quantity near the photon collecting unit in real time through the detecting unit, and the delay starting of the photon collecting unit is controlled through the delay unit, so that interference and noise in the measuring process can be effectively filtered, and real subcutaneous body fluid scattered photons can be obtained, thereby improving the accuracy and stability of the measuring result.

In some embodiments, the time required for the optical path may also be calculated in advance according to theory to determine the theoretical range of the delay time. For example: the distance of 1mm under the skin needs to be detected, the calculated light path is 2mm, the light speed in water is 22.5 KM/S, and the theoretical delay time can be 8.89ps. Because the delay time may be inconsistent due to the difference of different devices on the delay circuit, the delay time can be commonly determined and adjusted according to a predetermined theoretical range and the photon number monitored in real time, so as to adapt to the difference of different measuring devices, and adjust and optimize the delay time in real time. In other embodiments, the delay time may also be adjusted based on the minimum step length that the chip can adjust.

In some embodiments, photon receptors may be placed on pre-set detection points. Wherein the detection points may be arranged around the photon collection unit to monitor the number of photons around the photon collection unit. The photon acceptor may select a standard reflector with a lower transmittance. The number of photons received by the photon receptors is monitored simultaneously by the detection unit. When the number of photons received by the photon receptor is reduced to a certain threshold value, the photons scattered by the skin surface layer and the photons scattered by the air dust are judged to be basically disappeared at the moment, and the time delay can be ended. By monitoring the number of photons around the photon collection unit via the photon receptors, the delay time can be quickly determined and adjusted.

In an alternative embodiment, as shown in fig. 3, the raman spectrum measurement method may further include:

step S300: and controlling a time window after the time delay of the photon collecting unit through the time delay unit.

Step S302: and controlling the photon collecting unit to collect scattered photons of the medium to be measured, which accords with the expected target, by adjusting the time length of the time window.

In this embodiment, the switching of the photon collection unit may be controlled by a delay unit. The delay unit may send a pulse control signal to the photon collection unit. The photon collection unit is activated upon receipt of the pulse control signal. At the end of the pulse control signal, the photon collection unit is turned off. The photon collection unit may collect photons within the time window. And adjusting the length of the time window to enable the photon collecting unit to collect scattered photons of the medium to be detected, which accords with the expected target. Such as: collecting photons scattered by the medium to be detected with the thickness of 1mm-3mm under the skin, and collecting photons scattered by the medium to be detected with the thickness of 1mm-5mm under the skin. The time window is adjusted so that the measurement process is flexible and controllable, and the truly needed subcutaneous information can be acquired conveniently.

In an alternative embodiment, the raman spectrometry method may further comprise: repeating the following operations until the scattered photons of the medium to be tested meeting the preset quantity are obtained: outputting the pulse optical signal to the medium to be tested; and under the control of the delay unit, collecting scattered photons of the medium to be detected, which are generated when the pulse light signal reaches the medium to be detected, through the photon collecting unit.

On the one hand, when the optical signal irradiates the medium, most photons are subjected to Rayleigh scattering, and only one thousand of photons are subjected to Raman scattering, so that few detectable Raman scattered photons are generated. On the other hand, the intensity of the optical signal cannot be infinite because the absorption of photons by human skin generates heat, which would burn human skin, further limiting the number of detectable raman scattered photons. After single optical signal emission, the number of raman scattered photons collected by the photon collecting unit is small, and the requirement of raman spectrum measurement is difficult to meet.

In this embodiment, in order to collect enough raman scattered photons, the raman spectrum measurement process may be repeatedly performed multiple times, so that the photon collecting unit repeatedly collects the scattered photons of the medium to be measured, which are generated when the optical signal reaches the medium to be measured multiple times. And enough Raman scattered photons can be collected through multiple times of measurement, so that the reliability and accuracy of Raman spectrum measurement are effectively improved.

In an alternative embodiment, as shown in fig. 4, the raman spectrum measurement method may further include:

step S400: controlling the photon collecting unit to collect scattered photons of a target medium to be measured by adjusting the end time of the delay; the scattered photons of the target medium to be measured are generated when the pulse optical signal reaches the appointed depth in the medium to be measured.

In this embodiment, the delay end time, i.e., the turn-on time of the photon collection unit, may be adjusted. For example: when the number of photons falls to a second threshold, the control delay ends. Wherein the value of the second threshold may be determined from the subcutaneous 1mm optical path time. The number of photons drops to a first threshold value, indicating that the first medium scattered photons and the second medium scattered photons have substantially disappeared at this time. And the number of photons drops to a second threshold, it can be judged that the scattered photons of 1mm subcutaneously also substantially disappear. And at the moment, the time delay is finished and the photon collecting unit is started, and the photon collecting unit mainly collects photons scattered by the medium to be measured, which is deeper than 1mm below the skin. The second threshold may be adjusted according to the subcutaneous depth actually specified to be measured, and is not limited herein.

In this embodiment, by adjusting the delay time, the scattered photon interference of the medium to be measured with non-target depth can be reduced, and scattered photons generated by the medium to be measured with specified depth are mainly collected, so that subcutaneous body liquid layers with different depths are measured. Various interference amounts in the measuring process are filtered, signals actually required to be measured are collected, and accuracy and stability of a measuring result can be improved.

In an alternative embodiment, as shown in fig. 5, the raman spectrum measurement method may further include:

step S500: the light source control unit sends pulse signals to the photon output unit to control the working time of the photon output unit; wherein the operating time is the duration of the pulse signal.

In this embodiment, a pulse signal may be generated by the light source control unit and transmitted to the photon output unit. The photon output unit is turned on when receiving the pulse signal and outputs a beam of pulse light signal. After the pulse signal is finished, the photon output unit is turned off and cuts off the pulse light signal output. Thus, the on-time of the photon output unit is the duration of the pulse signal. The pulse signal is used for controlling the switch of the photon output unit, so that the working time of the photon output unit can be precisely adjusted.

In an alternative embodiment, the medium to be tested comprises subcutaneous body fluid of a specified depth. Therefore, the Raman spectrum measurement method can be used for measuring subcutaneous body fluid with a specified depth, such as subcutaneous 1mm and subcutaneous 2mm, so that the required characteristic information of the subcutaneous body fluid can be obtained rapidly, and the speed and accuracy of biochemical analysis are improved.

To make embodiments of the present application easier to understand, an exemplary application is provided below in connection with fig. 6A-6B. In an exemplary application, the raman spectrometry method may be applied in Yu Laman spectrometry apparatus. The raman spectrum measurement apparatus may include a photon output unit, a detection unit, a delay unit, a photon collection unit, and a light source control unit.

S11, the light source control unit generates short pulses and sends the short pulses to the photon output unit.

And S12, when receiving the short pulse, the photon output unit opens and outputs a pulse optical signal, and when the short pulse is ended, the pulse optical signal output is turned off.

After the pulse optical signal is emitted, the following processes are sequentially carried out:

(1) Photons enter the air, scatter and reflect when encountering dust, and reach the photon collecting unit after being scattered and reflected for a plurality of times. In the T0 phase, the number of photons near the photon collection unit gradually increases.

(2) The photons reach the skin surface to scatter and reflect, forming skin surface scattered photons. After several scattering and reflections, the photons then reach the photon collection unit. In the T1 stage, the number of photons near the photon collection unit is gradually stabilized and maintained.

(3) Some photons penetrate the skin surface layer and go deep under the skin, and scatter and reflect when encountering various subcutaneous components (such as body fluid and tissue fluid) to form scattered photons of the medium to be detected. And the scattered photons of the medium to be measured finally reach the photon collecting unit after being scattered and reflected for a plurality of times.

S13, the detection unit monitors the photon quantity in a preset range around the photon collection unit.

S14, the delay unit controls the photon collection unit to delay.

The delay corresponds to a T2 stage, and the photon collection unit is kept closed in the T0, T1 and T2 stages.

And S15, when the number of photons is reduced to a first threshold value, the delay unit finishes delay and sends a control pulse to the photon collecting unit.

S16, when the photon collecting unit receives the control pulse, the photon collecting unit starts and collects photons, and when the control pulse is finished, the photon collecting unit closes and stops collecting photons.

The photon collecting unit receives subcutaneous scattered photons at a receiving window, and the receiving window corresponds to a T3 stage.

S17, repeating the steps S11-S16 until scattered photons of the medium to be detected meeting the preset quantity are collected.

The raman spectrometry process is repeated with the photon collection unit operating repeatedly for a sufficient number of T3 phases to collect a sufficient number of photons.

Compared with the Raman spectrum measuring method known by the applicant, the Raman spectrum measuring method provided by the embodiment of the application can filter out various interference amounts in the measuring process, separate out signals actually required to be measured, improve the accuracy and stability of a measuring result, measure the Raman spectrum of subcutaneous body fluid layers with different depths, and facilitate biochemical analysis of the subcutaneous body fluids with different depths.

Example two

The embodiment of the application further provides the following technical solutions, and specific technical details can be referred to above.

A raman spectrum measurement apparatus 100 includes:

a photon output unit 1110 for outputting a pulse light signal; the pulse optical signal is used for irradiating the medium to be measured through the intermediate medium;

a photon collection unit 1310 for collecting photons;

a detecting unit 1200 for monitoring the number of photons within a preset range around the photon collecting unit 1310;

a delay unit 1300 for delaying a time window in which the photon collecting unit 1310 collects photons; wherein a delay time is dependent on the number of photons and an end time of the delay is later than an end time of the pulsed light signal;

the delayed time window corresponds to the time when the scattered photon of the medium to be measured reaches the photon collecting unit 1310, where the scattered photon of the medium to be measured is the scattered photon generated by the arrival of the pulse light signal at the medium to be measured.

In an alternative embodiment, the raman spectrum measuring device may further comprise a light source control unit 1100;

the light source control unit 1100 is configured to control an operation time of the photon output unit 1310 by a pulse signal; wherein the operating time is the duration of the pulsed light signal.

In an alternative embodiment, the delay unit 1300 is further configured to:

the time length of the time window is adjusted to control the photon collection unit 1310 to collect photons scattered by the medium under test that meet the desired target.

In an alternative embodiment, the delay unit 1300 is further configured to:

adjusting the end time of the delay, and controlling the photon collecting unit 1310 to collect scattered photons of the target medium to be measured; the scattered photons of the target medium to be measured are generated when the pulse optical signal reaches the appointed depth in the medium to be measured.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

Unless specifically stated or limited otherwise, the terms "mounted," "connected," "secured" and the like should be construed broadly, as they may be fixed, removable, or integral, for example; the device can be mechanically connected, electrically connected and communicated; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.

The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present application unless it is specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but should be considered part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.

It should also be noted that references in the specification to "one embodiment," "another embodiment," "an embodiment," etc., indicate that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment described generally in the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is intended that such feature, structure, or characteristic be implemented within the scope of the invention as set forth in connection with other embodiments.

In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to related descriptions of other embodiments.

It should be further noted that the foregoing is only a preferred embodiment of the present application, and is not intended to limit the scope of the patent protection of the present application, and all equivalent structures or equivalent process changes made by the content of the present application and the accompanying drawings, or direct or indirect application in other related technical fields, are included in the scope of the patent protection of the present application.

Claims (10)

1. A raman spectrum measurement method, characterized by being applied to a raman spectrum measurement apparatus including a photon output unit, a detection unit, a delay unit, and a photon collection unit, the method comprising:

outputting a pulse optical signal through the photon output unit, wherein the pulse optical signal is used for irradiating a medium to be detected; the pulse optical signals sequentially pass through a first medium, a second medium and the medium to be detected, and scatter in different mediums to respectively form a first medium scattered photon, a second medium scattered photon and a medium to be detected scattered photon;

monitoring the number of photons within a preset range around the photon collection unit through the detection unit;

controlling the delay of the photon collecting unit through the delay unit, wherein the delay time is determined according to the photon quantity; wherein the end time of the delay is later than the end time of the pulsed light signal;

and under the condition that the time delay is over, starting the photon collecting unit to collect photons scattered by the medium to be tested.

2. The raman spectroscopy method according to claim 1, wherein the method further comprises:

controlling a time window after the photon collecting unit delays through the delay unit;

and controlling the photon collecting unit to collect scattered photons of the medium to be measured, which accords with the expected target, by adjusting the time length of the time window.

3. The raman spectroscopy method according to claim 1, wherein the method further comprises:

repeating the following operations until the scattered photons of the medium to be tested meeting the preset quantity are obtained:

outputting the pulse optical signal to the medium to be tested;

and under the control of the delay unit, collecting scattered photons of the medium to be detected, which are generated when the pulse light signal reaches the medium to be detected, through the photon collecting unit.

4. The raman spectroscopy method according to claim 1, wherein the method further comprises:

controlling the photon collecting unit to collect scattered photons of a target medium to be measured by adjusting the end time of the delay; the scattered photons of the target medium to be measured are generated when the pulse optical signal reaches the appointed depth in the medium to be measured.

5. The raman spectrum measurement method according to claim 1, the raman spectrum measurement apparatus further comprising a light source control unit, characterized in that the method comprises:

the light source control unit sends pulse signals to the photon output unit to control the working time of the photon output unit;

wherein the operating time is the duration of the pulse signal.

6. A raman spectroscopy method according to any one of claims 1 to 5, wherein said medium to be measured comprises subcutaneous body fluid of a specified depth.

7. A raman spectroscopy apparatus comprising:

the photon output unit is used for outputting a pulse optical signal; the pulse optical signal is used for irradiating the medium to be measured through the intermediate medium;

a photon collection unit for collecting photons;

the detection unit is used for monitoring the photon quantity in a preset range around the photon collection unit;

a delay unit for delaying a time window in which the photon collection unit collects photons; wherein a delay time is dependent on the number of photons and an end time of the delay is later than an end time of the pulsed light signal;

the delayed time window corresponds to the time when the scattered photons of the medium to be detected reach the photon collecting unit, and the scattered photons of the medium to be detected are generated when the pulse light signals reach the medium to be detected.

8. The raman spectroscopy apparatus of claim 7, further comprising:

the light source control unit is used for controlling the working time of the photon output unit through a pulse signal;

wherein the operating time is the duration of the pulsed light signal.

9. The raman spectroscopy apparatus according to claim 7, wherein the delay unit is further configured to:

and adjusting the time length of the time window so as to control the photon collecting unit to collect scattered photons of the medium to be measured, which accords with the expected target.

10. The raman spectroscopy apparatus according to claim 7, wherein the delay unit is further configured to:

adjusting the ending time of the delay, and controlling the photon collecting unit to collect scattered photons of a target medium to be measured;

the scattered photons of the target medium to be measured are generated when the pulse optical signal reaches the appointed depth in the medium to be measured.