CN116496921A

CN116496921A - Endophyte for dissolving phosphorus and potassium and growth promotion application thereof

Info

Publication number: CN116496921A
Application number: CN202211490356.9A
Authority: CN
Inventors: 谭志远; 何璐; 张笑影; 彭桂香
Original assignee: South China Agricultural University
Current assignee: South China Agricultural University
Priority date: 2022-11-25
Filing date: 2022-11-25
Publication date: 2023-07-28
Anticipated expiration: 2042-11-25
Also published as: CN116496921B

Abstract

The invention relates to the technical field of microorganism technology and crop microbial inoculum fertilizer growth promotion, in particular to an endophyte for dissolving phosphorus and dissolving potassium, a planting fermentation liquor and growth promotion application of the planting fermentation liquor, wherein the endophyte is a Wittmann strain G62 which is preserved in the microorganism strain preservation center of Guangdong province at 10 month 22 of 2022, the strain preservation number is 62918, the strain is classified and named as Wittmann strain Weizmannia ginsengihumi, the preservation address is No. 100 of Pitot's first Zhonglu in Guangzhou province of Guangdong, the Wittmann strain G62 contains a 16SrDNA sequence shown as SEQ ID NO.1, and the strain can convert phosphorus and potassium element nutrients in soil into effective states for direct absorption and utilization by crops, is non-pathogenic and can grow in saline-alkali soil.

Description

Endophyte for dissolving phosphorus and potassium and growth promotion application thereof

Technical Field

The invention relates to the technical field of microorganism technology and crop microbial inoculum fertilizer growth promotion, in particular to endophytes for dissolving phosphorus and potassium, a planting fermentation liquid and growth promotion application of the planting fermentation liquid.

Background

Phosphorus is a component of compounds such as nucleoprotein, phospholipid and nucleic acid in plants, and is a main component of cell protoplasm and cell nucleus. The general seeds contain more phosphorus, the phosphorus can promote the growth of seedlings and the development of root systems, promote the early ripening of crops, plump seeds, enhance the cold resistance of overwintering crops, and play an important role in the processes of plant cell division and starch conversion into sugar.

Potassium can regulate the colloid state of protoplasm, promote crop to raise photosynthesis strength, promote monosaccharide to form saccharose, starch, cellulose, etc. and is closely related to sugar transportation. Therefore, sufficient potassium element is supplied to sugar crops such as beet, sweet potato and the like, and fruit trees, so that the sugar crops are not only robust in growth, but also the yield and sugar content are improved.

Potassium also increases the yield and quality of fiber crops such as cotton, hemp, etc., and increases the oil content of oil crops. Ensuring normal potassium nutrition, making the crop stem robust, enhancing lodging resistance of cereal crops, and enhancing cold resistance, drought resistance and disease resistance.

The contents of available phosphorus and quick-acting potassium in most of the soil in China for crops to directly absorb and utilize are low. In recent years, with the mass abuse of chemical fertilizers, excessive phosphate in the chemical fertilizers easily forms phosphate with trace elements such as iron, magnesium, calcium and the like in soil, so that the phosphate is difficult to be absorbed and utilized by crops, and excessive potassium in the chemical fertilizers also easily exists in potassium feldspar and mica in a mineral form, and is also difficult to be absorbed and utilized by crops. In the past, a large amount of fertilizer is applied, so that not only is the soil structure destroyed, but also the problem of soil hardening is increasingly serious, the utilization rate of the fertilizer is reduced, and the fertilizer cost is increased year by year.

Although the prior art utilizes microorganisms to degrade phosphorus and potassium, the current strain has pathogenicity or is difficult to survive in saline-alkali soil, and the degradation efficiency of phosphorus and potassium is affected.

Disclosure of Invention

One of the purposes of the invention is to avoid the defects in the prior art and provide an endophyte for dissolving phosphorus and potassium, which can convert the phosphorus and potassium element nutrients in soil into effective states for direct absorption and utilization by crops, has no pathogenicity and can grow in saline-alkali soil.

The second object of the present invention is to provide a planting fermentation liquid.

The third object of the present invention is to provide a growth promoting application of the planting fermentation liquid.

In order to achieve one of the above objects, the present invention provides the following technical solutions:

an endophyte for dissolving phosphorus and potassium is provided, wherein the endophyte is Weizmannia ginsengihumi G, namely a Wittmann strain G62, which is deposited in the microorganism strain collection of Guangdong province at 10 month 22 of 2022, the strain is classified and named as Wittmann strain Weizmannia ginsengihumi G62, the deposited address is Wittmann strain 100 in Guangzhou City of Guangdong, and the Wittmann strain G62 contains a 16SrDNA sequence shown in SEQ ID NO. 1.

In some embodiments, the Wittmania strain G62 has morphological, physiological and biochemical properties,

cell morphology characteristics: the cells of the Wittmania strain G62 are rod-shaped and are gram-positive bacteria;

colony morphology characterization: after growing for 24 hours at 37 ℃ in an LB culture medium plate, the bacterial colony is fine, round and smooth, white and opaque, and the proper growth temperature is 37 ℃ and the growth pH value is 6-8;

physiological and biochemical characteristics: facultative anaerobism, positive methyl red reaction, positive V.P reaction, negative urease reaction, negative ammonia production, positive catalase, positive indole reaction, incapacity of liquefying gelatin, negative nitric acid reduction, growth on nitrogen-free culture medium and tolerance of NaCl with concentration below 6%.

In some embodiments, the LB medium plate contains a modified medium, the components of which include peptone 9-12 g/L, yeast extract 4-6 g/L, naCl 9-12 g/L, sucrose 9-12 g/L; the culture conditions adopted are as follows: the temperature is 37 ℃, the rotating speed is 160-200 r/min, and the time is 24-48 h.

The endophyte for dissolving phosphorus and potassium has the beneficial effects that:

the endophyte for dissolving phosphorus and dissolving potassium is a Wittmann strain G62, the Wittmann strain G62 can dissolve the fixed phosphorus and potassium and other nutrient elements for direct absorption by crops, can reduce the application amount of chemical fertilizer, improve the utilization rate of the fertilizer, promote the ecological virtuous cycle of soil environment, save the cost of the fertilizer, promote the growth and development of plants, cope with the adverse environment and have no pathogenicity, can grow in saline-alkali soil, has strong adaptability, and is suitable for mass production and growth promotion application.

In order to achieve the second object, the present invention provides the following technical solutions:

provided is a planting fermentation liquid comprising bacterial liquid, wherein the bacterial liquid comprises a bacterial strain Bacillus siamensis J2, a bacterial strain Paenibacillus smacingsG 125 and the Wittmania bacterial strain G62.

In some embodiments, the bacterial liquid is cultured in a liquid culture medium containing the modified culture medium for 24-48 hours to obtain a planting fermentation liquid.

The planting fermentation liquor has the beneficial effects that:

the planting fermentation liquor disclosed by the invention has the advantages that the Weizhiman strain G62 and the strains Bacillus siamensis J2 and Paenibacillus smachusans G125 form the fermentation liquor, and the fermentation liquor can be co-cultured with other strains with growth promoting functions to form a mixed bacterial fertilizer, so that the crop growth capacity is better improved, and the mixed bacterial fertilizer can be widely promoted to grow and applied to agricultural production.

In order to achieve the third object, the present invention provides the following technical solutions:

the application of the planting fermentation liquid in promoting growth is provided, and the planting fermentation liquid is used for irrigating the planting fermentation liquid into saline-alkali soil.

In some embodiments, the planting broth is diluted 10-5 times and then irrigated.

The growth promotion application of the planting fermentation liquor has the beneficial effects that:

the growth promotion application of the plant fermentation liquid can realize the function of dissolving phosphorus and potassium only by irrigating the plant fermentation liquid in soil, and is convenient to operate.

Drawings

FIG. 1 is a graph of colonies after activation of Wettmania strain G62 by streaking on LB solid medium for 24 hours.

FIG. 2 is a graph of colonies of Wemtmannia strain G62 after three days of culture on phosphorus-containing PKO solid medium.

FIG. 3 is a graph showing colonies of the Wemtmannia strain G62 after three days of culture on potassium feldspar solid medium containing potassium.

FIG. 4 is a graph showing the mutual adaptability of the three strains G62, G125 and J2 of Wittmania after culturing them on LB solid medium for 24 hours.

FIG. 5 is a graph showing the effect of Wittmania strain G62 on rice growth, and each experiment was repeated three times. The left to right treatments are g62, g125+g62+j2, g125+j2, CK, respectively.

Detailed Description

Preferred embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While the preferred embodiments of the present invention are shown in the drawings, it should be understood that the present invention may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in this specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.

It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the invention. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

Reagents and materials used in the following examples are commercially available unless otherwise specified.

For ease of description, the following shorthand is used:

wettmania strain G62 is abbreviated as strain G62

Strain Bacillus siamensis J2 is abbreviated as strain J2

The strain Paenibacillus smacheransG 125 is abbreviated as strain G125

Example 1

Screening of strains

1. Bacterial strain separation, purification, preservation and screening

The method for separating and purifying the microorganism to obtain the phosphate-dissolving potassium from the paulownia tomentosa comprises the following steps:

wherein the culture medium used for separation is:

Pikovaskaia(PKO)(1.0L)：Glucose10.0g，(NH4) ₂ SO ₄ 0.5g，yeastextract0.5g，NaCl0.2g，KCl0.3g，MgSO ₄ ·7H ₂ O0.1g，FeSO ₄ 0.003g，MnSO ₄ 0.03g，Ca ₃ (PO ₄ ) ₂ 5.0g，pH6.8～7.0。

potassium feldspar medium (1.0L): mgSO (MgSO) ₄ ·7H ₂ O0.3g，Na ₂ HPO ₄ 0.5g，NaCl0.3g，CaCO ₃ 4.0g，NaSO ₄ 0.2g, glucose13.0g, potassium feldspar 3.0g, pH 6.8-7.0.

The culture medium used for the culture is:

modified LB (1.0L): 9-12 g/L peptone, 4-6 g/L yeast extract and 9-12 g/L NaCl, 9-12 g/L sucrose, pH 6-8, preferably 10g peptone, 5g yeast extract and 10g NaCl, 10g sucrose, pH 6.8-7.0.

The solid medium was added with agar20.0g, the semisolid medium was added with agar2.0g, and the mixture was autoclaved at 121℃for 20min. The culture conditions are as follows: the temperature is 37 ℃ and the time is 24-48 hours.

Cutting roots, stems and leaves of paulownia tomentosa into small sections by sterilized scissors, cleaning surface dust by using sterile water, firstly soaking the paulownia tomentosa in 2% sodium hypochlorite for 3min, then cleaning the paulownia tomentosa twice by using the sterile water, secondly soaking the paulownia tomentosa in 2.5% sodium thiosulfate for 1min, then cleaning the paulownia tomentosa twice by using the sterile water, soaking the paulownia tomentosa in 70% alcohol for 2min, then cleaning the paulownia tomentosa in 8 times by using the sterile water, each time for 2min, coating the sterile water after the last time on an LB plate, and observing the long fungus condition after two days to determine whether the disinfection is thorough.

And respectively cutting off 1cm from two ends of the sterilized tissue, respectively inoculating the sterilized tissue into semi-solid PKO and potassium feldspar culture mediums, after bacterial films grow out, selecting a sample with a transparent test tube, purifying the sample on the solid PKO and potassium feldspar culture mediums by a plate scribing method and a dilution plating method, and selecting a strain with a transparent ring for repeated subculture until the color, shape, size, texture and transparency of a bacterial colony are the same. Finally, the morphology is further observed through simple dyeing (stone carbonic acid reddish dyeing) and microscopic examination (oil mirror), and the conditions of uniform length, uniform width and uniform dyeing are unified as strain purification standards. The purified strain is picked by an inoculating loop, and is stored in a preservation solution (20% of glycerol, 1.5% of skimmed milk powder and 6ml of 0.5% bromophenol blue) in a refrigerator at-20 ℃ and-80 ℃ for standby, and finally the strain G62 with the capabilities of dissolving phosphorus and dissolving potassium is obtained.

FIG. 1 shows a colony chart of strain G62 after plate streaking on LB solid medium for 24 hours.

2. Quantitative detection of phosphorus and potassium dissolving functions:

(1) Quantitative detection of phosphorus dissolving function:

and detecting the content of soluble phosphorus in the strain fermentation broth by adopting a molybdenum-antimony colorimetric method so as to measure the phosphorus dissolving capacity of the strain G62.

Drawing a standard curve: accurately sucking 5.0mg/L phosphorus (K) ₂ HPO ₄ ) Standard solution 0.0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL and 10.0mL are respectively added into volumetric flasks with 50.0mL, corresponding equal volumes of blank solution and 2-3 drops of dinitrophenol indicator are respectively added, a proper amount of 100.0g/L sodium carbonate solution is added until the solution just presents slight yellow, after 5.0mL of molybdenum-antimony color development inhibitor is accurately added into each solution, shaking is carried out, distilled water is added to constant volume to 50mL, and then series standard solutions with phosphorus content of 0.0mg/L, 0.2mg/L, 0.4mg/L, 0.8mg/L and 1.0mg/L are respectively obtained. Shaking, and standing at room temperature for 30min. The absorbance of each concentration standard solution at the wavelength of 700nm is measured by a spectrophotometer, and a standard curve is drawn by taking the absorbance as an ordinate and the phosphorus concentration (mg/L) as an abscissa.

Molybdenum antimony stock solution: 153.0mL of concentrated sulfuric acid was slowly added to 400.0mL of distilled water, and the mixture was stirred continuously and cooled at room temperature. 10.0g of ammonium molybdate was weighed, dissolved in 300.0mL of water at 60℃and cooled. Slowly pouring the sulfuric acid solution into the ammonium molybdate solution, adding 100.0mL of 0.5% antimony potassium tartrate solution, cooling, adding water to dilute to 1000.0mL, shaking uniformly, and storing in a brown reagent bottle. The stock solution contained 1% ammonium molybdate and had a sulfuric acid content of 2.75mol/L.

Molybdenum-antimony color development resisting agent: 1.5g of ascorbic acid was weighed out and dissolved in 100.0mL of molybdenum antimony stock solution (with the preparation). 5.0mg/L phosphorus standard solution: 0.4394g of dried potassium dihydrogen phosphate is added into 100.0mL of water, then 5.0mL of concentrated sulfuric acid is slowly added, and the volume is fixed to 1000mL of water, thus obtaining the phosphorus (P) standard solution with the concentration of 100.0 mg/L. Absorbing 10.0mL of the above concentration solution into a volumetric flask of 200.0mL, adding water to a constant volume of 200mL to obtain a concentrateThe degree of concentration was 5.0mg/L of phosphorus (P) standard solution. Inoculating the activated strain G62 into PKO liquid culture medium, repeating the steps, culturing for 5 days at 37 ℃, centrifuging the fermentation liquor to be detected in a 4000r/min centrifuge for 10min, taking a proper amount of supernatant into a 50.0mL volumetric flask, diluting with water to about 3/5 of the total volume, adding 1-2 drops of dinitrophenol indicator into the solution, adding a proper amount of sodium carbonate solution with the concentration of 100.0G/L to regulate the solution to be slightly yellow, accurately absorbing 5.0mL of molybdenum-antimony color-developing agent into the reaction solution, shaking, adding water to fix the volume, and standing at room temperature for 30min. Measurement of the developed sample solution at OD with a spectrophotometer ₇₀₀ The instrument zero point was calibrated using a blank control (blank PKO liquid medium without bacterial cells) as a reference solution. Then, colorimetric determination of each reaction solution is carried out, and the absorbance OD is read ₇₀₀ And (3) calculating the phosphorus content in the strain fermentation broth according to a standard curve.

The test result shows that the phosphorus dissolving function of the strain G62 is higher, the average effective phosphorus content is 106.26 +/-0.77 mg/L, the CK control is 6.25+/-0.71 mg/L, and the ratio is increased by 17.00 times compared with the control, as shown in figure 2.

(2) And (3) quantitative detection of potassium dissolving function:

the soluble potassium content in the bacterial fermentation broth is measured by adopting a tetraphenyl and sodium borate method to measure the potassium decomposing capacity of the bacteria.

Drawing a standard curve: preparing 10mg/L potassium standard solution (KCl), adding 0.0mL, 1.0mL, 3.0mL, 5.0mL, 7.0mL and 9.0mL potassium standard solution into a 50mL volumetric flask, respectively adding 2mL formaldehyde solution with concentration of 30% and 2.0mL EDTA solution with concentration of 40g/L, accurately sucking 2.0mL tetraphenyl with concentration of 15g/L and sodium borate solution with a pipette, adding the solutions, shaking, and standing at room temperature for 20min. The solutions of each group are fully and evenly shaken, the solutions with different concentrations are respectively absorbed in a spectrophotometer, and the absorbance value of the solution with each concentration at the wavelength of 420nm is measured. And drawing a standard curve by taking the potassium content as an ordinate and the absorbance value as an abscissa.

Strain G62 was inoculated into a potassium-solubilizing functional assay liquid medium, and three replicates were each run with no strain inoculated into the potassium-solubilizing functional assay liquid medium as a blank. The strain was placed in a shaker and incubated at 37℃and 120rpm for 5 days with shaking. Collecting each group of culture solution in a sterile centrifuge tube, placing in a refrigerated centrifuge, centrifuging for 10min at 4 ℃ under 8000r/min, respectively sucking 5.0mL of supernatant of each strain culture solution in a 50mL volumetric flask, respectively adding 2.0mL of 30% formaldehyde solution and 2.0mL of 40g/L EDTA solution, shaking uniformly, adding 2.0mL of 15g/L tetraphenyl and sodium borate solution, shaking uniformly, and standing at room temperature for 20min. After the solutions of each group are fully and uniformly mixed, a proper amount of culture solution is sucked into a spectrophotometer, and the absorbance value of each solution at the wavelength of 420nm is measured. Taking the liquid culture medium without the inoculated strain as a blank control, recording the absorbance value of the culture solution, calculating the average value, and calculating the soluble potassium content in the fermentation liquid of the strain G62 according to the obtained standard curve.

The test result shows that the strain G62 has higher potassium-decomposing function, the average effective potassium content is 29.61+/-0.19 mg/L, the CK control is 2.82+/-0.13 mg/L, and the ratio is increased by 10.5 times compared with the control, as shown in figure 3.

Example 2

Physiological and biochemical characteristics and classification status of strain

Identification and preservation of strain G62: the strain G62 isolated in example 1 has the following morphological and physiological and biochemical properties:

a. cell morphology characteristics: the strain is rod-shaped.

b. Colony morphology characterization: the growth speed is faster on the modified LB plate, the colony is tiny, round and smooth, white and opaque (at 37 ℃ for 24 hours), and the diameter is 0.2-0.3cm. The optimum growth temperature was 37℃and the optimum growth pH was 7.

c. Physiological and biochemical characteristics: facultative anaerobism, positive methyl red reaction, positive V.P reaction, negative urease reaction, negative ammonia production, positive catalase, positive indole reaction, inability to liquefy gelatin, and negative nitrate reduction. Has the capability of dissolving phosphorus, potassium and the like. Can grow on a nitrogen-free culture medium and has good growth vigor on a sucrose-containing culture medium. pH growth range pH6-pH8; the concentration of NaCl resistance can reach 6 percent.

d. Determination of molecular classification status

Extracting DNA of the strain G62 obtained by separation in the example 1, amplifying a 16SrRNA gene, detecting by agarose gel, directly sequencing a PCR amplified product by a Guangzhou Tianyihuo technology Co., ltd, obtaining a 16SrRNA sequence of the strain, inputting the 16SrRNA sequence into GenBank for Blast comparison, and preliminarily determining the positions of genus and species of the strain G62 in taxonomy. As a result, it was found that the strain G62 of the present invention was 100% similar to the Weizmannian strain (Weizmanniagingihumi) model strain Gsoil 114. Thus, the strain G62 of the present invention was classified into Mannich Weizhi strain (Weizmannia ginsengihumi).

The Weizmannian bacteria (Weizmannian bacteria, engingihumi) G62 of the invention has been deposited at the Guangdong province microorganism strain collection (GDMCC) on 10 months 22 of 2022, and the strain collection number is 62918, and the classification name is Weizmannian bacteria (Weizmannia ginsengihumi), and the deposit address is Guangzhou City, guangdong province, first-violent Zhonglu 100.

Example 3

Strain adaptation to other strains

The strains G62, G125 and J2 are respectively activated on a modified LB culture medium, cultured for 24 hours at 37 ℃, and then dipped with a bamboo stick to form a triangle by respectively drawing a line on the modified LB culture medium and intersecting the two lines. Culturing at 37℃for 12 hours, and observing whether the intersections are connected. If all are connected, the strains can be co-cultured if the compatibility between the strains is good. As shown in FIG. 4, G62 and J2 and G125 can be co-cultured.

Example 4

Bacterial strain and other bacterial strain promoter

Activating the strains G62, G125 and J2 on a modified LB culture medium, culturing at 37 ℃ for 24 hours, and then respectively inoculating the strains into the modified liquid LB culture medium to culture until OD ₆₀₀ For 1, then set up experimental group one: sucking 0.3mL of G62 bacterial liquid to a new 2.7mL of modified liquid LB culture medium. Experimental group two: respectively sucking 0.1mL of G62 bacterial liquid, 0.1mL of G125 bacterial liquid and 0.1mL of J2 bacterial liquid toNew 2.7mL of modified liquid LB medium. Experimental group three: 0.15mL of G125 bacterial liquid and 0.15mL of J2 bacterial liquid are respectively sucked into new 2.7mL of modified liquid LB culture medium. The control group was 3mL of modified liquid LB medium without inoculation. Shake culturing at 37deg.C for 48 hr.

Selecting sun-dried sterilized soil, weighing 100g of the soil and 100g of Ca per pot ₃ (PO ₄ ) ₂ 1g and 1g of potassium feldspar, fully and uniformly stirring, adding proper amount of sterile water, and waiting for planting experiments.

Soaking Zheng drought variety rice seeds for 24h, placing germination paper for germination, selecting rice seedlings with a long potential difference on the 7 th day, adding the rice seedlings into the mixed soil, adding strain fermentation liquor 10ML diluted 20 times each time on the 1 st, 4 th, 7 th, 10 th and 13 th days after planting according to the set experimental groups, adding improved liquid LB culture medium 10ML diluted 20 times in a control group, and repeating each experiment three groups. Finally, the rice growth vigor of different treatments is observed in twenty-first days, and as shown in fig. 5, the mixed fermentation liquor added with the three strains is found to have the best growth vigor, and the mixed fermentation liquor added with the G62 fermentation liquor and the mixed fermentation liquor of the G125 and the J2 has a certain promotion effect compared with the control. The G62 fermentation liquor and the mixed fermentation liquor of other strains have certain growth promotion effect on rice in the soil containing phosphate and potassium ores.

SEQ ID NO.1 shows the following:

Claims

1. an endophyte for dissolving phosphorus and potassium, which is characterized in that: the endophyte is Weizmanniaginsichumi G62, namely Weizmannian strain G62, which is deposited in the Guangdong province microorganism strain collection at 10 month 22 of 2022, and has a strain deposit number of 62918, and is classified and named as Weizmanniaginsichumi, wherein the deposit address is Wizmanniaginsichumi No. 100 in Guangzhou, guangdong province, and the Weizmannian strain G62 contains a 16SrDNA sequence shown in SEQ ID NO. 1.

2. The endophyte for dissolving phosphorus and potassium according to claim 1, which is characterized in that: the Wittmania strain G62 has the following morphological, physiological and biochemical characteristics,

3. The endophyte for dissolving phosphorus and potassium according to claim 2, which is characterized in that: the LB culture medium plate contains an improved culture medium, wherein the components of the improved culture medium comprise 9-12 g/L of peptone, 4-6 g/L of yeast extract, 9-12 g/L of NaCl and 9-12 g/L of sucrose; the culture conditions of the Wittmania strain G62 in the modified culture medium are as follows: the temperature is 37 ℃, the rotating speed is 160-200 r/min, and the time is 24-48 h.

4. The planting fermentation liquor is characterized in that: comprising a bacterial fluid comprising the strain Bacillus siamensis J2, the strain Paenibacillus smaccerasers G125 and the Wittmania strain G62 of any one of claims 1-3.

5. The planting fermentation broth of claim 4, wherein: culturing the bacterial liquid in a liquid culture medium for 24-48 hours to obtain a planting fermentation liquid, wherein the liquid culture medium contains the improved culture medium.

6. The application of the planting fermentation liquor in promoting growth is characterized in that: the planting fermentation liquid according to claim 4 or 5 is used for irrigating the planting fermentation liquid into saline-alkali soil.

7. The growth promoting application of the plant fermentation broth according to claim 6, wherein the plant fermentation broth is characterized in that: and diluting the planting fermentation liquor by 10-5 times, and then irrigating.