CN116491418A - Cultivation and breeding method of kaempferia parvifolia - Google Patents

Abstract

The invention provides a cultivation and breeding method of kaempferia parvifolia, which comprises the following steps: inducing the adventitious bud of the rhizoma kaempferiae, proliferating the adventitious bud of the rhizoma kaempferiae, rooting culture of the rhizoma kaempferiae, hardening seedling and transplanting of the rhizoma kaempferiae. The method provided by the invention greatly shortens the seedling raising period and saves the production cost under the condition of keeping high proliferation rate and high survival rate.

Description

Cultivation and breeding method of kaempferia parvifolia

Technical Field

The invention relates to the technical field of plant cultivation, in particular to a cultivation and breeding method of kaempferia parvifolia.

Background

Rhizoma Kaempferiae (Kaempferia parviflora) is a perennial herb of the genus Kaempferia of the family Zingiberaceae, and is also known as Zingiber officinale or Zingiber zerumbet because its tubers are dark purple to black, which was originally reported in Burmese (Baker et al, 1890), and according to Tong Shaoquan (1995), wu Delin and Chen Zhongyi (1996), rhizoma Kaempferiae has no upper stems, rhizome meat blocks, outer skin light brown, dark purple cross section to black, leaf ellipsoids, inflorescences, flower mauve, white edges, no seeds, and is mainly produced in North Thailand northeast China, laos and southeast Asia bands. In the traditional medicine of thailand folk, its tubers have been used as a medicinal material for over 1000 years and are used for the treatment of various diseases. Pharmacological research shows that the kaempferia galanga can be used for treating male sexual dysfunction, enhancing muscle strength, regulating blood sugar and blood lipid, enhancing cognition, treating gastric ulcer, resisting depression, preventing cataract, vasodilation, protecting cardiovascular and the like, and has high medicinal value and great development and utilization potential.

At present, the small flower kaempferia galanga is widely cultivated and developed and utilized in southeast Asia, and as China is not the origin of the small flower kaempferia galanga, people have insufficient knowledge of the small flower kaempferia galanga, and lack of corresponding cultivation and breeding technology, only few plant gardens exist, such as: the Chinese academy of sciences south China plantations, the Chinese academy of sciences west double-plate nano tropical plantations and the Guangxi pharmaceutical plantations are introduced and cultivated, and the development and utilization of the small flower kaempferia galanga are still in a starting stage, and are only used as folk traditional medicines in partial villages of the Yunnan west double-plate nano. In order to fully develop the development and utilization potential of the small flower kaempferia galanga, the medicinal value of the small flower kaempferia galanga is widely popularized, and the problem of cultivation and breeding of the small flower kaempferia galanga in China is solved.

The tissue culture rapid propagation method of the kaempferia parviflora is disclosed by the environmental gardening research of the agricultural academy of sciences of Guangdong, and takes the bud basal part of the kaempferia parviflora as an explant, and the rapid propagation of the kaempferia parviflora is realized through the processes of pretreatment, bud basal part proliferation, cluster bud induction, subculture proliferation, rooting and seedling strengthening, seedling hardening transplanting and the like of the explant, wherein the survival rate of the explant is 80% at most, the bud basal part proliferation multiple is 8 times at most, the bud emergence index of the cluster buds is 5.8 at most, and the survival rate after seedling hardening transplanting is over 90%. However, this method is complicated in procedure, the whole procedure requires 125d at minimum, and the survival rate of explants is not high.

Disclosure of Invention

The invention aims to solve the defects of low propagation coefficient, long period, complex flow, high cost and the like of small-flower kaempferia galanga in the prior art, and provides a cultivation and breeding method for small-flower kaempferia galanga, which has the advantages of high propagation coefficient, simplified flow, high yield, rapid seedling culture, short cultivation period, production cost saving, ecological environment protection and resource protection.

A cultivation and breeding method of kaempferia parvifolia, which comprises the following steps:

inducing the adventitious bud of the rhizoma kaempferiae, proliferating the adventitious bud of the rhizoma kaempferiae, rooting culture of the rhizoma kaempferiae, hardening seedling and transplanting of the rhizoma kaempferiae.

Further, according to the cultivation and breeding method of the kaempferia parvifolia, the induction of the adventitious bud of the kaempferia parvifolia comprises the following steps:

(1) Obtaining bud bases:

collecting underground tubers of rhizoma Kaempferiae, germinating the cleaned, sterilized and sterilized underground tubers of rhizoma Kaempferiae at constant temperature, collecting the buds of rhizoma Kaempferiae when the buds grow to 3-4cm and the buds grow, and cutting the buds from the buds of rhizoma Kaempferiae;

(2) Disinfecting the bud base;

(3) Adventitious bud induction at the bud base:

inoculating the sterilized budlet base of rhizoma kaempferiae into adventitious bud induction culture medium, and inoculating 1 budlet base in each bottle;

(4) Culturing of the shoot basal part.

Further, according to the cultivation and breeding method of the kaempferia parviflora, the adventitious bud induction culture medium is as follows:

MS solid culture medium is used as basic culture medium, and sucrose 30g/L, agar 7g/L, hormone 6-BA 2mg/L, NAA0.1mg/L are added, and sterilized at 121deg.C for 20min.

Further, according to the cultivation and breeding method of the kaempferia parvifolia, the conditions for constant-temperature germination of the kaempferia parvifolia subsurface tubers are as follows: the temperature is 25 ℃ and the humidity is 80 percent.

Further, the cultivation and propagation method of kaempferia parvifolia as described above, the cultivation of the bud base comprises:

culturing the root of the grafted rhizoma kaempferiae bud in a culture room at 25 ℃ under 2000lx illumination time of 13h/d for 40d.

Further, according to the cultivation and propagation method of the kaempferia parvifolia, the propagation of the adventitious bud of the kaempferia parvifolia comprises the following steps:

(1) When the adventitious bud induced by the bud basal part grows to 3-4cm, transferring the adventitious bud in an ultra-clean workbench;

(2) Transferring the induced adventitious buds to an adventitious bud proliferation culture medium;

(3) After seed grafting, the seeds are placed in a culture room for culture, the temperature of the culture room is 25 ℃, the illumination intensity is 2500lx, the illumination time is 14h/d, and the culture is 20d.

Further, according to the cultivation and breeding method of the kaempferia parviflora, the adventitious bud proliferation culture medium is as follows:

MS solid culture medium is used as basic culture medium, and sucrose 30g/L, agar 8g/L, hormone 6-BA 2mg/L, NAA0.1mg/L, KT0.5 mg/L, and sterilizing at 121deg.C for 20min are added.

Further, the cultivation and breeding method of the kaempferia parvifolia as described above, wherein the rooting culture of the kaempferia parvifolia comprises:

(1) Cutting off adventitious buds when the proliferated adventitious buds grow to 5-6cm and 1-2 leaves are unfolded;

(2) Transferring the cut adventitious buds into a rooting medium, and inoculating 1 adventitious bud per bottle;

(3) And (3) culturing the adventitious buds after seed grafting in a culture chamber at a temperature of 25 ℃ for 20 days, wherein the illumination intensity is 4000lx and the illumination time is 14h/d.

Further, according to the cultivation and breeding method of the kaempferia parviflora, the rooting medium is as follows:

MS solid culture medium is used as basic culture medium, and sucrose 30g/L, agar 7g/L, NAA0.2mg/L and sterilizing at 121deg.C for 20min are added.

Further, according to the cultivation and breeding method of the kaempferia parvifolia, the seedling hardening and transplanting of the kaempferia parvifolia comprises the following steps:

(1) Hardening seedlings:

transferring the rooted kaempferia parviflora tissue culture seedling to a greenhouse for natural light irradiation, wherein the strong light irradiation needs to cover two layers of shading nets, the indoor temperature is 25 ℃, the air humidity is 80%, the cover is partially opened after 5d, the bottle cap is completely opened after 2d adaptation, and the seedling can be transplanted after 3d placement;

(2) Transplanting:

taking out the rhizoma kaempferiae culture seedling from the culture bottle, sterilizing, and transplanting to a plug, wherein the plug comprises the following matrix components: peat soil and perlite with the mass ratio of 1:1 are uniformly mixed, then the substrate is sterilized by using 15% carbendazim solution, the film can be used after being covered for two days, the planting depth is 2-3 cm, the substrate is used for covering root systems, and water is poured;

after 30d of culture, transplanting the culture medium into a nutrition pot, wherein the matrix in the nutrition pot is peat soil and perlite 1 in a mass ratio of 4:1, and the humidity is 80%.

The cultivation and breeding method of the kaempferia parvifolia has the following beneficial effects:

1. the cultivation and breeding method provided by the invention only comprises 4 steps of induction of the adventitious bud of the rhizoma kaempferiae, multiplication of the adventitious bud of the rhizoma kaempferiae, rooting culture of the rhizoma kaempferiae, hardening seedling transplanting of the rhizoma kaempferiae, and the like, omits steps of cluster bud induction, subculture multiplication and the like, has simple flow, only needs 80 days from the basal part of the rhizoma kaempferiae bud to the obtaining of the aseptic seedling with root of the rhizoma kaempferiae with the quantity of 25 times, shortens 45 days compared with the prior art, and greatly shortens the seedling raising period of the rhizoma kaempferiae;

2. according to the invention, the adventitious buds are induced by adopting the root parts of the kaempferia parvifolia buds, so that the initiation rate of the adventitious buds induced by the root parts of the buds is high and reaches 93.75%, and the number of buds reaches 4-6;

3. the proliferation coefficient of the adventitious buds is high, and 1 adventitious bud 20d can proliferate 7.8 new adventitious buds; in the prior art, only 5.8 new adventitious buds can be proliferated by 1 adventitious bud;

4. the rooting rate is high, and the rooting rate reaches 95.25% after the adventitious bud is cultured for 20 d;

5. the transplanting survival rate is high, and the survival rate reaches 100% after 15d transplanting.

In conclusion, the method provided by the invention greatly saves seedling raising time under the condition of keeping high survival rate and high proliferation rate.

Drawings

In order to more clearly illustrate the invention or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is an image of a subsurface tuber of Kaempferia parvifolia;

FIG. 2 is a bud image of Kaempferia parvifolia;

FIG. 3 (a) is a schematic view of the bud base inoculated according to an embodiment of the present invention;

FIG. 3 (b) is a schematic diagram of a shoot base inoculated according to an embodiment of the present invention;

FIG. 4 (a) is a schematic diagram of the basal portion of a bud induced by an embodiment of the present invention;

FIG. 4 (b) is a schematic diagram of a shoot apex induced by an embodiment of the present invention;

FIG. 5 (a) is a schematic diagram of adventitious bud inoculation according to an embodiment of the present invention;

FIG. 5 (b) is a second schematic diagram of adventitious bud inoculation according to an embodiment of the present invention;

FIG. 6 (a) is a schematic diagram of an adventitious bud induced by an embodiment of the present invention;

FIG. 6 (b) is a second schematic diagram of adventitious bud induced by an embodiment of the present invention;

FIG. 7 is an image of the experimental results corresponding to CK in Table 6;

FIG. 8 is an image of the experimental results corresponding to C-1 in Table 6;

FIG. 9 is an image of the experimental results corresponding to C-2 in Table 6;

FIG. 10 is an image of the experimental results corresponding to C-3 in Table 6;

FIG. 11 is an image of the experimental results corresponding to C-4 in Table 6;

FIG. 12 is a kaempferia galanga tissue culture Miao Tuxiang in a culture chamber;

FIG. 13 is an image of a Kaempferia galanga tissue culture seedling after opening the bottle cap;

FIG. 14 is a washed Kaempferia galanga tissue culture Miao Tuxiang;

fig. 15 is an image of a kaempferia galanga tissue culture seedling transplanted into a tray.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The technical problems to be solved by the invention include the following two aspects:

(1) The kaempferia parviflora is a vegetative propagation crop, and in the long-term vegetative propagation process, various viruses are infected and accumulated in vivo, so that the yield is reduced, the quality is reduced, the stress resistance is weakened, and various diseases are caused. And by utilizing the tissue culture rapid propagation technology, the kaempferia parviflora can be subjected to detoxification and bacteria removal treatment, so that the excellent properties of the kaempferia parviflora can be recovered, and the yield and quality of the kaempferia parviflora can be improved. The method provided by the invention simplifies the process of tissue culture and rapid propagation, greatly saves time, adjusts the hormone concentration of adventitious bud induction and adventitious bud proliferation, improves the survival rate of explants and the proliferation coefficient of adventitious buds, improves the seedling hardening and transplanting method, and greatly improves the transplanting survival rate.

(2) The conventional tuber propagation has larger demand for tubers, lower propagation coefficient and higher planting cost. By inducing the adventitious buds of the kaempferia parviflora, the invention realizes the advantages of high propagation coefficient and high propagation speed, so that the method provided by the invention can obtain a large number of tissue culture seedlings in a short period, and the planting cost of the kaempferia parviflora is reduced.

The cultivation and breeding method of kaempferia parvifolia provided by the invention is described in detail as follows:

induction of adventitious bud of Kaempferia parvifolia

(1) Test materials

The root of kaempferia parviflora bud.

(2) Test method

(1) The method comprises the steps of obtaining and collecting underground tubers of rhizoma kaempferiae with bud bases, as shown in figure 1, cleaning sediment under tap water, soaking and sterilizing in 2000 times potassium permanganate diluent for 30min, taking out and airing. And (3) placing the dried tubers into river sand after high-temperature sterilization and cooling, then placing the river sand into a climatic incubator with the temperature of 25 ℃ and the humidity of 80% for constant-temperature germination, and collecting the buds of the kaempferia parvifolia when the buds grow to 3-4cm and the bud bases slightly expand, as shown in figure 2. The collected budlet of kaempferia parviflora is washed for 2h under running water, and then placed in an ultra-clean workbench to cut the basal part of the budlet.

(2) Sterilization of bud bases

On an ultra-clean workbench, placing the cut bud basal part in 70% alcohol for sterilization for 30s, vibrating while sterilizing to ensure that the sterilizing liquid is fully contacted with the bud basal part, sterilizing more thoroughly, flushing for 3 times by using sterile water, placing the bud basal part in 0.1% mercuric chloride solution for sterilization for 12min, vibrating while sterilizing, shaking in sterile water for 5min after sterilization, flushing for 3 times by using sterile water, and placing the bud basal part on sterile filter paper for airing after flushing.

(3) Inoculation of shoot basal portion

On an ultra-clean bench, the bud bases were placed in sterilized dishes, the portions of the surfaces of the bud bases in contact with the sterilizing liquid were cut off in the dishes, and the resulting dish was cut into a size of 0.5cm×0.5cm, inoculated in an adventitious bud induction medium supplemented with a hormone concentration as shown in Table 1, and 1 bud base was inoculated in each bottle, as shown in FIG. 3 (a) and FIG. 3 (b). The basal medium is MS solid medium, sucrose 30g/L, agar 7g/L, hormone 6-BA and NAA are added, and sterilization is carried out for 20min at 121 ℃.

TABLE 1 adventitious bud induction Medium

(4) Culture of shoot basal portion

After seed grafting, the seeds are placed in a culture chamber for culture, and the temperature of the culture chamber is 25 ℃, the illumination intensity is 2000lx and the illumination time is 13h/d as shown in FIG. 4 (a) and FIG. 4 (b). After culturing for 40d, the pollution rate, the starting rate and the bud number are counted.

Contamination rate = number of contaminated vials/number of vaccinated vials x 100%

Start rate = number of flasks started/(number of flasks vaccinated-number of flasks contaminated) ×100%

(3) The test results are shown in Table 2

Table 2 experimental results

As can be seen from Table 2, the contamination rates for each group were low, between 5.00% and 8.33%, with an average contamination rate of 7.00%. The induction rate of each group of adventitious buds is between 68.75% and 93.75%, the bud emergence number is between 1.6 and 3.64, the bud induction rate of a control group without any hormone is at least 68.75%, the bud emergence number is at least 1 to 2, the induction of the adventitious buds can be promoted by adding 6-BA and NAA with certain concentration, and when the NAA concentration is 0.1mg/L, the induction rate of the adventitious buds and the bud emergence number are reduced after being increased along with the increase of the 6-BA concentration, and when the 6-BA concentration is 2mg/L, the induction rate of the adventitious buds reaches 93.75%, and the bud emergence number reaches 4 to 6. Therefore, the optimal hormone ratio of 2mg/L6-BA+0.1mg/L NAA is preferable in the invention.

(II) proliferation of adventitious bud of Kaempferia parvifolia

(1) Test materials

Adventitious bud of Kaempferia parvifolia.

(2) Test method

(1) Acquisition of adventitious bud

When the adventitious bud induced at the bud base grows to 3-4cm, the adventitious bud is transferred in an ultra clean bench.

(2) Switching of adventitious buds

The induced adventitious buds were transferred onto an ultra-clean bench to an adventitious bud proliferation medium to which hormone concentrations shown in Table 3 were added, and 1 adventitious bud was inoculated per bottle as shown in FIG. 5 (a) and FIG. 5 (b). The basal medium is MS solid medium, sucrose 30g/L, agar 8g/L, hormone 6-BA, KT and NAA are added, and sterilization is carried out for 20min at 121 ℃.

TABLE 3 adventitious bud proliferation Medium

After seed grafting, the seeds are placed in a culture room for culture, the temperature of the culture room is 25 ℃, the illumination intensity is 2500lx, and the illumination time is 14h/d. As shown in FIG. 6 (a) and FIG. 6 (b), after 20d of cultivation, the contamination rate and the adventitious bud proliferation coefficient were counted.

Proliferation coefficient = number of newly grown adventitious buds/number of inoculated adventitious buds

The test results are shown in Table 4

TABLE 4 proliferation results of adventitious buds of Kaempferia parvifolia

As is clear from Table 4, after 20d of cultivation, the contamination rate of each treatment group was low, and the proliferation factor of each treatment group of adventitious buds was between 2.3 and 6.8, wherein the proliferation factor of adventitious buds reached the maximum at a hormone concentration of 2 mg/L6-BA+0.5 mg/LKT+0.1mg/L NAA, and at this hormone concentration, 1 adventitious bud could proliferate to grow 6 to 7 new adventitious buds. Therefore, the optimal hormone ratio of 2mg/L6-BA+0.1mg/L NAA is preferable in the present invention.

Rooting culture of Kaempferia parviflora

(1) Test materials

Adventitious buds of Kaempferia parviflora.

(2) Test method

(1) Acquisition of adventitious bud

When the proliferated adventitious bud grows to 5-6cm and 1-2 leaves are spread, the adventitious bud is transferred.

(2) Switching of adventitious buds

On an ultra-clean workbench, the proliferated adventitious buds are placed in a sterilized dish, the adventitious buds are cut, the cut adventitious buds are transferred to rooting culture medium added with hormone concentration shown in table 4, and 1 adventitious bud is inoculated per bottle. The basal medium is MS solid medium, sucrose 30g/L, agar 7g/L, and hormone NAA are added, and sterilized at 121deg.C for 20min.

TABLE 5

(3) Rooting culture

After seed grafting, the seeds are placed in a culture room for culture, the temperature of the culture room is 25 ℃, the illumination intensity is 4000lx, and the illumination time is 14h/d. After 20d of culture, the pollution rate, the rooting rate, the single Miao Genshu and the root length are counted.

(3) Test results

TABLE 6 rooting induction experiment results of kaempferia parvifolia

Treating pollution rate rooting rate/%average rooting number/average root growth rooting condition

As can be seen from Table 6 and FIGS. 7-11, the contamination rate of each treatment group was 0; the rooting rate of each group is between 53.85 and 95.25 percent, the rooting rate of a control group without any hormone is 75.00 percent, and the rooting rate and the root condition are obviously affected by adding a certain NAA. With the increase of NAA concentration, the rooting rate is firstly increased and then decreased, when the NAA concentration is increased to 0.2mg/L, the rooting rate is up to 95.25%, the NAA concentration is continuously increased, and the rooting rate is obviously decreased; the average root number of each group is between 0.80 and 4.93, the average root length is between 0.4 and 2.0cm, the number of root systems is at most 4.93 when NAA concentration is 0.1mg/L, the length of the root systems is at most 1.9cm, the length of the root systems is at most 2.0cm when NAA concentration is 0.2mg/L, and the number of the root systems is at most 3.50. Therefore, the invention finally selects the optimal hormone proportion of the NAA of 0.2mg/L for the rooting medium.

(IV) seedling hardening and transplanting of Kaempferia parvifolia

(1) Hardening seedlings:

hardening off is needed before transplanting the tissue culture seedlings of the kaempferia parviflora, so that the tissue culture seedlings are adapted to the external environment, and the survival rate of the tissue culture seedlings after transplanting is improved. Transferring the rooted buddleia officinalis tissue culture seedlings to a greenhouse for natural light irradiation, covering two layers of shading nets with strong light irradiation, wherein the indoor temperature is 25 ℃, the air humidity is 80%, opening the bottle cap part for cultivating the buddleia officinalis tissue culture seedlings after 5d, completely opening the bottle cap after 2d adaptation, and transplanting after 3d placement as shown in fig. 13 and 14.

(2) Transplanting:

taking out rhizoma Kaempferiae culture seedling from culture flask with long tweezers, cleaning the culture medium with water, as shown in figure 14, without damaging root system, soaking in 0.5% potassium permanganate solution for 30s for sterilization, transplanting into tray, as shown in figure 15, mixing matrix with peat soil and perlite at a ratio of 1:1, sterilizing with 15% carbendazim solution, covering with film for two days, using, planting with depth of 2-3 cm, covering root system with matrix, and watering thoroughly. Two layers of shading nets are covered under natural illumination, shading is not needed in rainy days, and the statistical survival rate can reach 100% after 15d transplanting. The nutrition space of the plug tray after 30d culture can not meet the growth requirement of seedlings, the seedlings need to be transplanted into nutrition bowls with the diameter of 9cm and the height of 9cm in time, the matrix is peat soil and perlite, the proportion is 4:1, the humidity is 80%, the seedlings are planted in the middle of the nutrition bowls, the nutrition bowls are filled with the matrix, water is poured through, and then daily management is carried out. And thereafter can be transplanted into the field as desired.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. The cultivation and breeding method of kaempferia parvifolia is characterized by comprising the following steps of:

inducing the adventitious bud of the rhizoma kaempferiae, proliferating the adventitious bud of the rhizoma kaempferiae, rooting culture of the rhizoma kaempferiae, hardening seedling and transplanting of the rhizoma kaempferiae.

2. The cultivation and propagation method of kaempferia parvifolia according to claim 1, wherein the induction of adventitious buds of kaempferia parvifolia comprises:

(1) Obtaining bud bases:

collecting underground tubers of rhizoma Kaempferiae, germinating the cleaned, sterilized and sterilized underground tubers of rhizoma Kaempferiae at constant temperature, collecting the buds of rhizoma Kaempferiae when the buds grow to 3-4cm and the buds grow, and cutting the buds from the buds of rhizoma Kaempferiae;

(2) Disinfecting the bud base;

(3) Adventitious bud induction at the bud base:

inoculating the sterilized budlet base of rhizoma kaempferiae into adventitious bud induction culture medium, and inoculating 1 budlet base in each bottle;

(4) Culturing of the shoot basal part.

3. The cultivation and propagation method of kaempferia parviflora according to claim 2, wherein the adventitious bud induction medium is:

MS solid culture medium is used as basic culture medium, and sucrose 30g/L, agar 7g/L, hormone 6-BA 2mg/L, NAA0.1mg/L are added, and sterilized at 121deg.C for 20min.

4. The cultivation and propagation method of kaempferia parviflora according to claim 2, wherein the conditions for constant temperature germination of the subsurface tubers of kaempferia parviflora are as follows: the temperature is 25 ℃ and the humidity is 80 percent.

5. The cultivation and propagation method of kaempferia parvifolia according to claim 2, wherein the cultivation of the bud base comprises:

culturing the root of the grafted rhizoma kaempferiae bud in a culture room at 25 ℃ under 2000lx illumination time of 13h/d for 40d.

6. The cultivation and propagation method of kaempferia parvifolia according to any one of claims 1 to 5, wherein the proliferation of the adventitious bud of kaempferia parvifolia comprises:

(1) When the adventitious bud induced by the bud basal part grows to 3-4cm, transferring the adventitious bud in an ultra-clean workbench;

(2) Transferring the induced adventitious buds to an adventitious bud proliferation culture medium;

(3) After seed grafting, the seeds are placed in a culture room for culture, the temperature of the culture room is 25 ℃, the illumination intensity is 2500lx, the illumination time is 14h/d, and the culture is 20d.

7. The cultivation and propagation method of kaempferia parvifolia according to claim 6, wherein the adventitious bud proliferation medium is:

MS solid culture medium is used as basic culture medium, and sucrose 30g/L, agar 8g/L, hormone 6-BA 2mg/L, NAA0.1mg/L, KT0.5 mg/L, and sterilizing at 121deg.C for 20min are added.

8. The cultivation and propagation method of kaempferia parvifolia according to any one of claims 1 to 5, wherein rooting cultivation of kaempferia parvifolia comprises:

(1) Cutting off adventitious buds when the proliferated adventitious buds grow to 5-6cm and 1-2 leaves are unfolded;

(2) Transferring the cut adventitious buds into a rooting medium, and inoculating 1 adventitious bud per bottle;

(3) And (3) culturing the adventitious buds after seed grafting in a culture chamber at a temperature of 25 ℃ for 20 days, wherein the illumination intensity is 4000lx and the illumination time is 14h/d.

9. The cultivation and propagation method of kaempferia parvifolia according to claim 8, wherein the rooting medium is:

MS solid culture medium is used as basic culture medium, and sucrose 30g/L, agar 7g/L, NAA0.2mg/L and sterilizing at 121deg.C for 20min are added.

10. The cultivation and propagation method of kaempferia parvifolia according to any one of claims 1 to 5, wherein the seedling hardening and transplanting of kaempferia parvifolia comprises:

(1) Hardening seedlings:

transferring the rooted kaempferia parviflora tissue culture seedlings to a greenhouse for natural light irradiation, covering two layers of shading nets with strong light irradiation, wherein the indoor temperature is 25 ℃, the air humidity is 80%, opening the bottle cap part for cultivating the kaempferia parviflora tissue culture seedlings after 5d, completely opening the bottle cap after 2d adaptation, and transplanting after 3d placement;

(2) Transplanting:

taking out the rhizoma kaempferiae culture seedling from the culture bottle, sterilizing, and transplanting to a plug, wherein the plug comprises the following matrix components: peat soil and perlite with the mass ratio of 1:1 are uniformly mixed, then the substrate is sterilized by using 15% carbendazim solution, the film can be used after being covered for two days, the planting depth is 2-3 cm, the substrate is used for covering root systems, and water is poured;

after 30d of culture, transplanting the culture medium into a nutrition pot, wherein the matrix in the nutrition pot is peat soil and perlite with the mass ratio of 4:1, and the humidity is 80%.