CN116482137B - New coronavirus rapid detection device and detection method - Google Patents
New coronavirus rapid detection device and detection method Download PDFInfo
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- CN116482137B CN116482137B CN202310383936.6A CN202310383936A CN116482137B CN 116482137 B CN116482137 B CN 116482137B CN 202310383936 A CN202310383936 A CN 202310383936A CN 116482137 B CN116482137 B CN 116482137B
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- 238000001514 detection method Methods 0.000 title claims abstract description 83
- 241000711573 Coronaviridae Species 0.000 title claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 102
- 230000007704 transition Effects 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 23
- 238000010894 electron beam technology Methods 0.000 claims description 17
- 238000005070 sampling Methods 0.000 claims description 13
- 238000005507 spraying Methods 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 239000007921 spray Substances 0.000 claims description 11
- 238000007599 discharging Methods 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 230000005684 electric field Effects 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 4
- 238000003909 pattern recognition Methods 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 abstract description 3
- 241000700605 Viruses Species 0.000 description 13
- 241000894006 Bacteria Species 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 230000003321 amplification Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011202 physical detection method Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/20—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials
- G01N23/20058—Measuring diffraction of electrons, e.g. low energy electron diffraction [LEED] method or reflection high energy electron diffraction [RHEED] method
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2223/00—Investigating materials by wave or particle radiation
- G01N2223/60—Specific applications or type of materials
- G01N2223/612—Specific applications or type of materials biological material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application discloses a novel coronavirus rapid detection device, which comprises a vacuum detection chamber, wherein a track, a slide conveying mechanism, an image amplifying display mechanism and an image recognition system are arranged in the vacuum detection chamber; the novel coronavirus rapid detection device has higher automation degree and can effectively reduce the infection probability of detection personnel.
Description
Technical Field
The application relates to the technical field of medical detection equipment, in particular to a novel coronavirus rapid detection device.
Background
The new coronavirus is a single-stranded positive strand RNA virus, belongs to the genus beta virus of the family coronaviridae, can generate interpersonal transmission, and has stronger transmission capability. The common pathogen detection methods include pathogen culture, serum antibody detection, nucleic acid detection and the like, but the pathogen culture is long in time consumption and low in efficiency, and the serum antibody detection specificity is low, so that the nucleic acid detection method which is short in time consumption, strong in specificity and higher in sensitivity is generally adopted clinically.
At present, the mainstream detection method is a PCR technology, namely a real-time fluorescence quantitative PCR technology, which belongs to a second generation PCR technology and can quantitatively detect nucleic acid, and the method utilizes an amplification curve generated during nucleic acid amplification to judge the nucleic acid quantity, but the method still needs 2 hours of processes, needs to collect specimens for loading together, cannot be used as scattered specimens, and cannot be used for emergency projects.
To overcome the above-mentioned drawbacks, the chinese patent of publication No. CN111454839a discloses a new coronavirus nucleic acid preparation and PCR detection integrated device, which can be used as a scattered specimen and also can meet the emergency requirements, but according to the description of the paragraph [ 0047 ] of the specification, DNA amplification is started only by adding, each sample is set for a period of 1 hour, fluorescence is generated by excitation of the laser emitter 52, a fluorescence signal is received by the fluorescence signal detection device 53, and analyzed by the data processing and control element 54, and finally outputted to the data output panel 55 for display.
Disclosure of Invention
Aiming at the defects in the prior art, the application provides a novel coronavirus rapid detection device which replaces the chemical detection mode of the conventional PCR technology in a physical detection mode, truly realizes rapid detection, namely detection and knowledge, greatly improves the timeliness of detection, is beneficial to finding a virus carrier in time, and cuts off a transmission chain.
The technical scheme of the application is as follows:
the application provides a novel coronavirus rapid detection device, which is characterized in that: the device comprises a vacuum detection chamber, wherein a track, a slide conveying mechanism, an image amplifying display mechanism and an image recognition system are arranged in the vacuum detection chamber, and the device comprises:
the vacuum detection chamber is internally provided with a vacuum environment;
the image amplifying display mechanism comprises an electron gun, an electrostatic high-voltage generator, a deflection electrode and a fluorescent screen, wherein the electron gun is used for generating electron beams, the electrostatic high-voltage generator is used for accelerating electrons and enabling the electrons to deviate by a preset angle, and the deflection electrode is used for generating a circular electric field with deflection voltage U and radius R; the electron is at a direct velocity V 0 The electron beam after scattering can be irradiated to a slide sampling area and projected to a fluorescent screen to form an amplified image;
the track passes through the image amplifying display mechanism, the fluorescent screen is positioned on one side of the track, and the electron gun and the deflection electrode are positioned on the other side of the track;
the slide conveying mechanism is arranged on the track and can clamp the slide body, and the slide conveying mechanism is used for enabling the slide body to move along the track and is also used for enabling the slide body to be lifted;
the image recognition system comprises a high-speed camera and a computer, wherein the high-speed camera is used for shooting an enlarged image displayed on a fluorescent screen and transmitting the photo to the computer in real time, and the shooting speed of the high-speed camera is matched with the moving speed of the slide body along the track; the computer is used for receiving and storing photos from the high-speed camera and splicing the single photos into an integral graph according to the actual positions of the single photos; the method is also used for carrying out pattern recognition on the single photo and the preset new coronavirus characteristics; and the method is also used for calculating the diameter of the circular arc and comparing the circular arc with the preset new coronavirus characteristics.
Preferably, the central area of the slide body is a sampling area, the sampling area is divided into a first unit area array of x rows and y columns, and any one of the first unit areas is divided into a second unit area array of m rows and n columns; during detection, the slide conveying mechanism drives the slide body to move, so that scattered electron beams are sequentially irradiated onto each first unit area or each second unit area.
Preferably, when the scattered electron beam irradiates the first unit area array or the second unit area array, the track of the slide conveying mechanism driving the slide body to move is S-shaped.
Preferably, the slide conveying mechanism comprises a first motor, a gear, a bearing seat, a lifting mechanism and a clamping mechanism, wherein the lifting mechanism is arranged on a fixing part of the bearing seat, the clamping mechanism is arranged at a lifting end of the lifting mechanism and is used for clamping the slide body, the gear is fixed with a rotating part of the bearing seat, an output end of the first motor is fixed with a central hole of the gear and is used for driving the gear to rotate, the gear is limited in a track, the lifting mechanism is arranged above the track, the motor is arranged below the track, and the clamping mechanism clamps the slide body from two sides of one side of the slide body bearing a sample.
Preferably, the track comprises two sub-tracks which are oppositely arranged left and right, a space is arranged between the two sub-tracks, the space is larger than the outer diameter of the bearing seat and smaller than the outer diameter of the gear, racks are arranged in the two sub-tracks, and the racks in the two sub-tracks are meshed with the gear.
Preferably, the track is specifically a U-shaped formed by sequentially connecting a feeding section, a detecting section and a discharging section, the detecting section is a horizontal straight line, the electron gun is positioned on a midvertical line of the detecting section, the fluorescent screen is parallel to the detecting section and is arranged right opposite to the electron gun, the feeding section is a straight line which is inclined downwards, the feeding section and the discharging section are both vertical to the detecting section and are in fillet transition at a joint, a feeding transition chamber is arranged at a position where the feeding section intersects with a side wall of the vacuum detecting chamber, and a discharging transition chamber is arranged at a position where the feeding section intersects with the side wall of the vacuum detecting chamber.
Preferably, a sample fixing device is further arranged between the feeding transition chamber and the detection section, the sample fixing device comprises a reciprocating mechanism and a spraying mechanism, the clamping mechanism is hinged to the output end of the lifting mechanism, the reciprocating mechanism is used for pushing the clamping mechanism to drive the slide body to swing in a reciprocating manner, and the spraying mechanism is used for spraying the organic setting agent to the surface of the slide body after shaking of the slide body is completed.
Preferably, the reciprocating mechanism comprises a cam and a second motor, the second motor is used for driving the cam to rotate, the cam is arranged on one side of the clamping mechanism, and the cam can push the slide body to realize reciprocating shaking in the rotating process; the spraying mechanism comprises a liquid storage tank, a liquid pump and a spray head, wherein the input end of the liquid pump is communicated with the liquid storage tank, the output end of the liquid pump is communicated with a spray head pipeline, and the liquid outlet direction of the spray head faces towards one surface of the slide body for bearing a sample.
Preferably, the lifting mechanism is specifically a hydraulic cylinder, the hydraulic cylinder is rotatably installed on the bearing seat, a first auxiliary rack is arranged on 1/4 circumference of the outer side wall of the hydraulic cylinder, a second auxiliary rack is arranged above the rounded corner transition part of the feeding section and the detecting section, and the first auxiliary rack is matched with the second auxiliary rack.
Preferably, the feeding transition chamber and the feeding transition chamber are respectively provided with an outer gate on one side close to the outside of the vacuum detection chamber, and an inner gate on one side close to the inside of the vacuum detection chamber.
The beneficial effects of the application are as follows:
the rapid detection device for the novel coronavirus adopts a physical detection method, irradiates viruses and bacteria by utilizing scattered electron beams, projects the viruses and bacteria onto a fluorescent screen, realizes high-power physical amplification of the viruses and the bacteria, then is matched with an image recognition system, performs confirmation and recognition according to inherent characteristics of the viruses and the bacteria, can realize detection of a single specimen, and can realize immediate detection and immediate knowledge of results, and the detection effect is rapid and accurate; the novel coronavirus rapid detection device has higher automation degree and can effectively reduce the infection probability of detection personnel.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. Like elements or portions are generally identified by like reference numerals throughout the several figures. In the drawings, elements or portions thereof are not necessarily drawn to scale.
FIG. 1 is a top view of a vacuum detection chamber of a novel coronavirus rapid detection device provided by the present application (slide drive and sample fixture are not shown);
FIG. 2 is a side view of the track-in section of the rapid detection device of the present application;
FIG. 3 is a front view of the track feed section of the rapid detection device of the present application;
in the drawings, 1-vacuum detection chamber, 2-track, 21-in section, 22-detection section, 23-out section, 24-rack, 31-first motor, 32-gear, 33-hydraulic cylinder, 331-first auxiliary rack, 34-clamping mechanism, 41-electron gun, 42-electrostatic high voltage generator, 43-deflection electrode, 44-screen, 51-high speed camera, 52-computer, 6-slide body, 61-sampling area, 71-second motor, 72-cam, 73-liquid tank, 74-liquid pump, 75-shower head, 81-in transition chamber, 82-out transition chamber, 83-outside gate, 84-inside gate, 9-second auxiliary rack.
Detailed Description
In order that those skilled in the art will better understand the present application, a technical solution in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, shall fall within the scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate in order to describe the embodiments of the application herein.
In the present application, the azimuth or positional relationship indicated by the terms "upper", "lower", "inner", and the like are based on the azimuth or positional relationship shown in the drawings. These terms are only used to better describe the present application and its embodiments and are not intended to limit the scope of the indicated devices, elements or components to the particular orientations or to configure and operate in the particular orientations.
Also, some of the terms described above may be used to indicate other meanings in addition to orientation or positional relationships, for example, the term "upper" may also be used to indicate some sort of attachment or connection in some cases. The specific meaning of these terms in the present application will be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, the terms "disposed," "configured," "connected," "secured," and the like are to be construed broadly. For example, "connected" may be in a fixed connection, a removable connection, or a unitary construction; may be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements, or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.
In addition, the term "plurality" shall mean two as well as more than two.
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
The embodiment provides a novel coronavirus rapid detection device, as shown in fig. 1, comprising a vacuum detection chamber 1, wherein a track 2, a slide conveying mechanism, an image amplifying display mechanism and an image recognition system are arranged in the vacuum detection chamber 1, wherein:
the vacuum detection chamber 1 is internally provided with a vacuum environment;
the image amplifying display mechanism comprises an electron gun 41, an electrostatic high-voltage generator 42, a deflection electrode 43 and a fluorescent screen 44, wherein the electron gun 41 is used for generating electron beams, the electrostatic high-voltage generator 42 is used for accelerating electrons and enabling the electrons to deviate by a preset angle, the deflection electrode 43 is used for generating a circular ring-shaped electric field with deflection voltage U and radius R, and the circular ring-shaped electric field is used for enabling the electron beams to generate single-side 45-degree scattering; electrons are emitted at a direct velocity V0, scattered by the annular electric field, and the scattered electron beams can be irradiated onto a slide sampling region 61 and projected onto a fluorescent screen 44 to form an enlarged image;
the track 2 passes through the image magnifying display mechanism, the fluorescent screen 44 is positioned on one side of the track 2, and the electron gun 41 and the deflection electrode 43 are positioned on the other side of the track 2;
the slide conveying mechanism is arranged on the track 2 and can clamp the slide body 6, and the slide conveying mechanism is used for enabling the slide body 6 to move along the track 2 and is also used for enabling the slide body 6 to be lifted;
the image recognition system comprises a high-speed camera 51 and a computer 52, wherein the high-speed camera 51 is used for shooting the enlarged image displayed on the fluorescent screen 44 and transmitting the photo to the computer 52 in real time, and the shooting speed of the high-speed camera 51 is matched with the moving speed of the slide body 6 along the track 2; the computer 52 is used for receiving and storing the photos from the high-speed camera 51, and is also used for splicing the individual photos into an integral graph according to the actual positions; the method is also used for carrying out pattern recognition on the single photo and the preset new coronavirus characteristics; and the method is also used for calculating the diameter of the circular arc and comparing the circular arc with the preset new coronavirus characteristics.
The rapid detection device for the novel coronavirus provided by the embodiment is a physical detection device, irradiates viruses and bacteria by utilizing scattered electron beams, projects the viruses and bacteria onto the fluorescent screen 44, realizes high-power physical amplification of the viruses and the bacteria, then is matched with an image recognition system, performs confirmation and recognition according to inherent characteristics of the viruses and the bacteria, can realize detection of a single specimen, and can realize quick and accurate detection effect as long as the result is detected; in addition, the novel coronavirus rapid detection device has higher automation degree, and can effectively reduce the infection probability of detection personnel.
In this embodiment, the central area of the slide body 6 is a sampling area 61, the sampling area 61 is divided into a first unit area array of x rows and y columns, and any first unit area is divided into a second unit area array of m rows and n columns; during detection, the slide conveying mechanism drives the slide body 6 to move so that scattered electron beams are sequentially irradiated onto each first unit area or each second unit area; when the scattered electron beam irradiates the first unit area array or the second unit area array, the trajectory of the slide conveying mechanism driving the slide body 6 to move is S-shaped. When the slide body 6 reaches the detection position under the drive of the slide conveying mechanism, the scattered electron beam just projects an image of the first unit area with the position coordinates of (1, 1) onto the fluorescent screen 44, the high-speed camera 51 takes a first photo, then the slide conveying mechanism drives the slide body 6 to move to the first unit area with the position coordinates of (2, 1) so that the scattered electron beam just projects the image of the first unit area onto the fluorescent screen 44, the high-speed camera 51 immediately takes a second photo … …, and so on, and the slide conveying mechanism drives the slide body 6 to sequentially reach the first unit area with the position coordinates of (y, 1), then the first unit area with the position coordinates of (y, 2), the first unit area with the position coordinates of (y-1, 2) until all x y first unit areas are traversed, and each first unit area is taken a next enlarged photo by the high-speed camera 51; if the first unit area with a certain position coordinate is to be rechecked, after returning to the position, shooting the second unit area on the first unit area according to a similar S-shaped track in the first unit area until all the m multiplied by n second unit areas are shot.
In this embodiment, as shown in fig. 2 to 3, the slide conveying mechanism includes a first motor 31, a gear 32, a bearing seat, a lifting mechanism and a clamping mechanism 34, the lifting mechanism is disposed on a fixing portion of the bearing seat, the clamping mechanism 34 is disposed at a lifting end of the lifting mechanism, the clamping mechanism 34 is used for clamping the slide body 6, the gear 32 is fixed with a rotating portion of the bearing seat, an output end of the first motor 31 is fixed with a central hole of the gear 32 and is used for driving the gear 32 to rotate, the gear 32 is limited in the track 2, the lifting mechanism is disposed above the track 2, the motor is disposed below the track 2, and the clamping mechanism 34 clamps the slide body 6 from two sides of one side of the slide body 6 bearing a sample; the track 2 comprises two sub-tracks 2 which are oppositely arranged left and right, a distance is arranged between the two sub-tracks 2, the distance is larger than the outer diameter of the bearing seat and smaller than the outer diameter of the gear 32, racks 24 are arranged in the two sub-tracks 2, and the racks 24 in the two sub-tracks 2 are meshed with the gear 32. When the slide conveying mechanism is started, the first motor 31 drives the gear 32 to rotate, and the gear 32 is limited in the track 2 and meshed with the track 2, so that the gear 32 can move along the track 2 under the drive of the first motor 31, and the lifting mechanism and the clamping mechanism 34 can not rotate along with the gear 32 but can move along with the gear through the arrangement of the bearing seat.
In this embodiment, as shown in fig. 1, the track 2 is specifically in a U shape formed by sequentially connecting a feeding section 21, a detecting section 22 and a feeding section 23, the detecting section 22 is a horizontal straight line, the electron gun 41 is located on a midvertical line of the detecting section 22, the fluorescent screen 44 is arranged parallel to the detecting section 22 and is opposite to the electron gun 41, the feeding section 21 is a straight line inclined downward, the feeding section 21 and the feeding section 23 are both perpendicular to the detecting section 22 and are in rounded transition at a joint, a feeding transition chamber 81 is arranged at a position where the feeding section 21 intersects with a side wall of the vacuum detecting chamber 1, and a feeding transition chamber 82 is arranged at a position where the feeding section 23 intersects with the side wall of the vacuum detecting chamber 1; when the slide body 6 is obliquely arranged in the feeding section 21, the surface of the slide body 6 bearing the sample faces upwards, and the sample fixing device can be arranged above the feeding section 21.
In this embodiment, a sample fixing device is further disposed between the feeding transition chamber 81 and the detection section 22, the sample fixing device includes a reciprocating mechanism and a spraying mechanism, the clamping mechanism 34 is hinged with the output end of the lifting mechanism, the reciprocating mechanism is used for pushing the clamping mechanism 34 to reciprocate with the slide body 6, and the spraying mechanism is used for spraying the organic setting agent to the surface of the slide body 6 after the shaking of the slide body 6 is completed; the slide body 6 is subjected to shaking for a plurality of times, the amplitude of which is not more than 1mm, and then a preset amount of organic setting agent is sprayed for fixation, so that the virus stacking degree can be reduced as much as possible, and the projection visual effect is improved;
specifically, as shown in fig. 2 to 3, the reciprocating mechanism includes a cam 72 and a second motor 71, the second motor 71 is used for driving the cam 72 to rotate, the cam 72 is arranged on one side of the clamping mechanism 34, the cam 72 can push the slide body 6 to realize reciprocating shake in the rotating process, and the shake amplitude can be controlled within 1mm through reasonable selection of the second motor 71 and the cam 72; the spraying mechanism comprises a liquid storage tank 73, a liquid pump 74 and a spray head 75, wherein the input end of the liquid pump 74 is communicated with the liquid storage tank 73, the output end of the liquid pump 74 is communicated with the spray head 75 through a pipeline, and the liquid outlet direction of the spray head 75 faces to one surface of the slide body 6 for bearing samples.
In this embodiment, as shown in fig. 1 to 3, the lifting mechanism is specifically a hydraulic cylinder 33, the hydraulic cylinder 33 is rotatably mounted on a bearing seat, a first auxiliary rack 33124 is arranged on 1/4 circumference of the outer side wall of the hydraulic cylinder 33, a second auxiliary rack 924 is arranged above the rounded corner transition of the feeding section 21 and the detecting section 22, and the first auxiliary rack 33124 is matched with the second auxiliary rack 924; when the gear 32 passes the rounded transition of the feeding section 21 and the detecting section 22, the first auxiliary rack 33124 and the second auxiliary rack 924 are engaged, and the hydraulic cylinder 33 is rotated by 90 ° under the action of the auxiliary rack 24, so that the side carrying the sample faces away from the screen 44 toward the electron gun 41 when the slide body 6 passes the screen 44.
In this embodiment, as shown in fig. 1 to 2, the feeding transition chamber 81 and the discharging transition chamber 82 are each provided with an outer gate 83 on a side close to the outside of the vacuum detection chamber 1, and an inner gate 84 on a side close to the inside of the vacuum detection chamber 1, so that the feeding transition chamber 81 and the feeding transition chamber 81 can perform a transition function by such a setting, which is advantageous for the construction of the vacuum environment in the vacuum detection chamber 1.
The working principle of the embodiment is as follows:
after sampling, the slide body 6 and the clamping mechanism 34 of the slide driving mechanism are assembled outside the vacuum detection chamber 1, then the first motor 31 of the slide driving device is started to drive the gear 32 to rotate and move along the track 2, the outer gate 83 sent into the transition chamber 81 is opened, the inner gate 84 is closed, after the slide driving mechanism passes through the outer gate 83, the outer gate 83 is closed, the inner gate 84 is opened, and the slide driving mechanism brings the slide body 6 into the vacuum detection chamber 1;
when the slide body 6 moves obliquely downwards in the feeding section 21 of the track 2 and reaches the position of the sample fixing device, the first motor 31 pauses to work, the slide body 6 is still in an upward inclined state, the second motor 71 of the reciprocating mechanism starts the driving cam 72 to rotate, the cam 72 pushes the clamping mechanism 34 to drive the slide body 6 to shake slightly, then the second motor 71 is closed, the spraying mechanism is started, the liquid pump 74 sprays the organic shaping agent in the liquid storage tank 73 to the sampling area 61 of the slide body 6 through the spray head 75 to shape the sample on the liquid storage tank, then the first motor 31 continues to work, the slide body 6 continues to move, and the slide body 6 is deflected by the first auxiliary rack 33124 and the second auxiliary rack 924 and enters the detection section 22;
during detection, the slide body 6 firstly reaches a preset position between the electron gun 41 and the fluorescent screen 44, then the high-speed camera 51 shoots and displays the amplified image of ten thousand times of the first unit area of the position coordinates (1, 1) of the sampling area 61 of the slide body 6 on the fluorescent screen 44, the amplified image is transmitted and stored in the computer 52, then the slide driving mechanism drives the slide body 6 to move at a constant speed according to a preset S-shaped track, the high-speed camera 51 is matched to shoot the amplified image of ten thousand times of each first unit area in sequence, the computer 52 sequentially shoots and stores the amplified image of each first unit area in the computer 52, the arc diameter of each received amplified image is calculated after the arc characteristic is identified, the first unit area with the arc diameter of the amplified image of 0.6-1.4mm is taken as the area to be detected again, if the computer 52 obtains a negative detection result, and the sample leaves the vacuum detection chamber 1 along the numerical control automatic track 2 through the transition chamber 82;
if the computer 52 obtains the positions of a plurality of areas to be rechecked, the slide driving mechanism drives the slide body 6 to sequentially go to the positions of the areas to be rechecked, in each rechecked area, a hundred thousand times of amplified images of the second unit areas of the position coordinates (1, 1) of the areas to be rechecked are shot by the high-speed camera 51 and are transmitted and stored in the computer 52, and then the slide driving mechanism drives the slide body 6 to move at a constant speed according to a preset S-shaped track, and the hundred thousand times of amplified images of each second unit area of the areas to be rechecked are shot by the high-speed camera 51 and are transmitted and stored in the computer 52; then the sample follows the numerical control automatic track 23 to come to the position of the next region to be rechecked, and the above work is repeated until the shooting of all second unit regions of all the positions of the region to be rechecked is completed; amplifying the image of the single second unit area received by the computer 52, calculating the circular arc diameter after identifying the circular arc characteristics, and taking the second unit area with the circular arc diameter of 6-14mm of the amplified image as a hit area;
if the arc characteristics are not identified, or no region to be rechecked appears; alternatively, no hit area occurs; the detection result of the sample is negative;
if the arc feature is identified and a hit area appears, the detection result of the sample is positive;
in other cases, the secondary detection may be repeated, and if it is a viral carrier, the number of viruses per exhalation is relatively large, and the probability of overlapping occlusion of all viruses for two consecutive samples is relatively low.
For the special coronal characteristics of the novel coronavirus, the novel coronavirus can be represented by theoretically electron irradiation, and if the calculation contrast of the circular arc diameter is added on the basis of the coronavirus, the accuracy of the detection result is necessarily improved greatly, and the existence of the novel coronavirus can be determined almost 100%.
It should be noted that, in this embodiment, the electron generation amount, the electron emission initial speed, and the offset voltage for deflecting the electrons of the electron gun 41 are all adjustable, and if the preset value of the apparatus does not reach the desired effect, the parameters may be manually adjusted, and the desired effect may be achieved by matching with the adjustment of the nc automatic rail 2.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the same; although the application has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the application, and are intended to be included within the scope of the appended claims and description.
Claims (5)
1. A new coronavirus rapid detection device is characterized in that: the device comprises a vacuum detection chamber, wherein a track, a slide conveying mechanism, an image amplifying display mechanism and an image recognition system are arranged in the vacuum detection chamber, and the device comprises:
the vacuum detection chamber is internally provided with a vacuum environment;
the image amplifying display mechanism comprises an electron gun, an electrostatic high-voltage generator, a deflection electrode and a fluorescent screen, wherein the electron gun is used for generating electron beams, the electrostatic high-voltage generator is used for accelerating electrons and enabling the electrons to deviate by a preset angle, and the deflection electrode is used for generating a circular electric field with deflection voltage U and radius R; the electron is at a direct velocity V 0 The electron beam after scattering can be irradiated to a slide sampling area and projected to a fluorescent screen to form an amplified image;
the track passes through the image amplifying display mechanism, the fluorescent screen is positioned on one side of the track, and the electron gun and the deflection electrode are positioned on the other side of the track;
the slide conveying mechanism is arranged on the track and can clamp the slide body, and the slide conveying mechanism is used for enabling the slide body to move along the track and is also used for enabling the slide body to be lifted;
the image recognition system comprises a high-speed camera and a computer, wherein the high-speed camera is used for shooting an enlarged image displayed on a fluorescent screen and transmitting the photo to the computer in real time, and the shooting speed of the high-speed camera is matched with the moving speed of the slide body along the track; the computer is used for receiving and storing photos from the high-speed camera and splicing the single photos into an integral graph according to the actual positions of the single photos; the method is also used for carrying out pattern recognition on the single photo and the preset new coronavirus characteristics; the method is also used for calculating the diameter of the circular arc and comparing the circular arc with the preset new coronavirus characteristics;
the slide conveying mechanism comprises a first motor, a gear, a bearing seat, a lifting mechanism and a clamping mechanism, wherein the lifting mechanism is arranged on a fixed part of the bearing seat, the clamping mechanism is arranged at a lifting end of the lifting mechanism, the clamping mechanism is used for clamping a slide body, the gear is fixed with a rotating part of the bearing seat, an output end of the first motor is fixed with a central hole of the gear and is used for driving the gear to rotate, the gear is limited in a track, the lifting mechanism is arranged above the track, the motor is arranged below the track, and the clamping mechanism clamps the slide body from two sides of one surface of the slide body bearing a sample;
the track is composed of two sub-tracks which are oppositely arranged left and right, a space is arranged between the two sub-tracks, the space is larger than the outer diameter of the bearing seat and smaller than the outer diameter of the gear, racks are arranged in the two sub-tracks, and the racks in the two sub-tracks are meshed with the gear;
the track is in a U shape formed by sequentially connecting a feeding section, a detecting section and a discharging section, the detecting section is a horizontal straight line, the electron gun is positioned on the middle vertical line of the detecting section, the fluorescent screen is arranged parallel to the detecting section and is opposite to the electron gun, the feeding section is a straight line which is inclined downwards, the feeding section and the discharging section are both vertical to the detecting section and are in fillet transition at the joint, a feeding transition chamber is arranged at the position where the feeding section intersects with the side wall of the vacuum detecting chamber, and a discharging transition chamber is arranged at the position where the feeding section intersects with the side wall of the vacuum detecting chamber;
a sample fixing device is further arranged between the feeding transition chamber and the detection section, the sample fixing device comprises a reciprocating mechanism and a spraying mechanism, the clamping mechanism is hinged with the output end of the lifting mechanism, the reciprocating mechanism is used for pushing the clamping mechanism to drive the slide body to swing in a reciprocating manner, and the spraying mechanism is used for spraying an organic setting agent to the surface of the slide body after the shake of the slide body is completed;
the reciprocating mechanism comprises a cam and a second motor, the second motor is used for driving the cam to rotate, the cam is arranged on one side of the clamping mechanism, and the cam can push the slide body to realize reciprocating shaking in the rotating process; the spraying mechanism comprises a liquid storage tank, a liquid pump and a spray head, wherein the input end of the liquid pump is communicated with the liquid storage tank, the output end of the liquid pump is communicated with a spray head pipeline, and the liquid outlet direction of the spray head faces towards one surface of the slide body for bearing a sample.
2. The rapid detection device for new coronaviruses according to claim 1, wherein: the central area of the slide body is a sampling area, the sampling area is divided into a first unit area array of x rows and y columns, and any one of the first unit areas is divided into a second unit area array of m rows and n columns; during detection, the slide conveying mechanism drives the slide body to move, so that scattered electron beams are sequentially irradiated onto each first unit area or each second unit area.
3. A rapid detection device for a new coronavirus according to claim 2, wherein: when the scattered electron beam irradiates the first unit area array or the second unit area array, the track of the slide conveying mechanism driving the slide body to move is S-shaped.
4. The rapid detection device for new coronaviruses according to claim 1, wherein: the lifting mechanism is specifically a hydraulic cylinder, the hydraulic cylinder is rotatably installed on a bearing seat, a first auxiliary rack is arranged on 1/4 circumference of the outer side wall of the hydraulic cylinder, a second auxiliary rack is arranged above the fillet transition part of the feeding section and the detecting section, and the first auxiliary rack is matched with the second auxiliary rack.
5. The rapid detection device for new coronaviruses according to claim 1, wherein: the feeding transition chamber and the feeding transition chamber are respectively provided with an outer gate on one side close to the outside of the vacuum detection chamber, and an inner gate on one side close to the inside of the vacuum detection chamber.
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| CN110186943A (en) * | 2018-02-22 | 2019-08-30 | Fei 公司 | Smart pre-scan in scanning transmission charged particle microscope |
| CN112381817A (en) * | 2020-11-30 | 2021-02-19 | 中国科学院自动化研究所 | Rapid virus detection system combining scanning electron microscope transmission mode and transmission electron microscope |
| CN113866977A (en) * | 2021-10-20 | 2021-12-31 | 滕州市中等职业教育中心学校 | Automatic conveying equipment for microscope slide glass for medical examination |
| CN114878619A (en) * | 2022-05-24 | 2022-08-09 | 福建博奥医学检验所有限公司 | Intelligent detection device for viruses |
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| JP4154300B2 (en) * | 2003-09-08 | 2008-09-24 | 株式会社日立ハイテクノロジーズ | Transmission electron microscope system and inspection method using the same |
| US11593938B2 (en) * | 2020-10-04 | 2023-02-28 | Borrirs Pte. Ltd. | Rapid and automatic virus imaging and analysis system as well as methods thereof |
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| CN110186943A (en) * | 2018-02-22 | 2019-08-30 | Fei 公司 | Smart pre-scan in scanning transmission charged particle microscope |
| CN112381817A (en) * | 2020-11-30 | 2021-02-19 | 中国科学院自动化研究所 | Rapid virus detection system combining scanning electron microscope transmission mode and transmission electron microscope |
| CN113866977A (en) * | 2021-10-20 | 2021-12-31 | 滕州市中等职业教育中心学校 | Automatic conveying equipment for microscope slide glass for medical examination |
| CN114878619A (en) * | 2022-05-24 | 2022-08-09 | 福建博奥医学检验所有限公司 | Intelligent detection device for viruses |
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