CN116421499B - Composite green pricklyash peel volatile oil nano-alcohol plastid with antibacterial, anti-inflammatory and repairing effects, and preparation method and application thereof - Google Patents
Composite green pricklyash peel volatile oil nano-alcohol plastid with antibacterial, anti-inflammatory and repairing effects, and preparation method and application thereof Download PDFInfo
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- CN116421499B CN116421499B CN202211371248.XA CN202211371248A CN116421499B CN 116421499 B CN116421499 B CN 116421499B CN 202211371248 A CN202211371248 A CN 202211371248A CN 116421499 B CN116421499 B CN 116421499B
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- volatile oil
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- camphor tree
- green pricklyash
- nanoalcohol
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- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61Q17/005—Antimicrobial preparations
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Abstract
The invention discloses a composite green pricklyash volatile oil nanoalcohol body with antibacterial, anti-inflammatory and repairing effects, and a preparation method and application thereof, wherein the nanoalcohol body comprises the following components in parts by mass: 0.5-5 parts of phospholipid, 5-30 parts of micromolecular polyalcohol, 0.5-4 parts of green pricklyash peel volatile oil, 0.5-2 parts of ceramide E, 0.5-5 parts of camphor tree extract and water to make up to 100 parts of total mass. The invention also discloses a preparation method of the composite green pricklyash peel volatile oil nano-alcohol plastid. The composite green pricklyash volatile oil nano alcohol plastid is mild, has no stimulation, good anti-inflammatory, repairing and antibacterial effects and good storage stability.
Description
Technical Field
The invention relates to the field of nano preparations, in particular to a composite green pricklyash peel volatile oil nano alcohol plastid with antibacterial, anti-inflammatory and repairing effects, and a preparation method and application thereof.
Background
Green pricklyash peel is mainly distributed in asian countries including china. The green pricklyash peel contains abundant volatile oil, alkaloid, amide, coumarin, lignan and other components, and has antibacterial, antiviral, analgesic, immunity enhancing, and cardiovascular protecting effects. However, the green pricklyash peel volatile oil has strong irritation, and when the green pricklyash peel volatile oil is used as an active ingredient of a skin care product, skin irritation or other adverse reactions are easy to cause. In addition, the green pricklyash peel oil also has the problems of unstable light/heat and difficult absorption, and greatly limits the application of the green pricklyash peel volatile oil in the field of cosmetics.
Disclosure of Invention
The primary aim of the invention is to overcome the defects of the prior art and provide the composite green pricklyash peel volatile oil nano-ethosome with antibacterial, anti-inflammatory and repairing effects, and the nano-ethosome is mild, non-irritating and stable in storage.
The invention further aims at providing a preparation method of the composite green pricklyash peel volatile oil nano-ethosome with antibacterial, anti-inflammatory and repairing effects.
The invention also aims to provide application of the composite green pricklyash peel volatile oil nano-ethosome with antibacterial, anti-inflammatory and repairing effects.
In order to solve the technical problems, the invention adopts the following technical scheme: the composite green pricklyash volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects comprises the following components in parts by mass: 0.5-5 parts of phospholipid, 5-30 parts of micromolecular polyalcohol, 0.5-4 parts of green pricklyash peel volatile oil, 0.5-2 parts of ceramide E, 0.5-5 parts of camphor tree extract and water to make up to 100 parts of total mass.
As a preferred scheme, the composite green pricklyash volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects comprises the following components in parts by mass: 0.5-2 parts of phospholipid, 10-25 parts of micromolecular polyalcohol, 1-3 parts of green pepper volatile oil, 0.5-1.5 parts of ceramide E and 1-3 parts of camphor tree extract, wherein the total mass part of the components is 100 parts; more preferably, the composite green pricklyash volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects comprises the following components in parts by mass: 1 part of phospholipid, 20 parts of small molecular polyalcohol, 2 parts of green pepper volatile oil, 1 part of ceramide E, 2 parts of camphor tree extract and 74 parts of water.
Preferably, the phospholipid comprises one or more of soybean lecithin, hydrogenated lecithin, sphingomyelin and phosphatidylcholine.
Preferably, the small molecule polyalcohol comprises one or more of 1, 3-propanediol, glycerol, 1, 2-butanediol, pentanediol and 1, 2-hexanediol; more preferably, the small molecule polyol is 1, 2-hexanediol.
As a preferable scheme, the camphor tree extract is a mixture of camphor tree bark oil and camphor tree leaf extract, and the mass ratio of the camphor tree bark oil is as follows: camphor tree leaf extract = 1-5:1; more preferably, the mixture mass ratio of the camphor tree bark oil and the camphor tree leaf extract is that the camphor tree bark oil: camphor tree leaf extract = 1:1.
The invention also provides a preparation method of the composite green pricklyash peel volatile oil nano-ethosome with antibacterial, anti-inflammatory and repairing effects, which comprises the following steps:
(1) Mixing phospholipid, small molecular polyalcohol, pericarpium Zanthoxyli volatile oil, ceramide E and camphor tree extract to obtain organic phase;
(2) Adding the organic phase in the step (1) into water, and mixing to obtain a mixed solution;
(3) Homogenizing and dispersing the mixed solution obtained in the step (2) to obtain crude ethosome;
(4) Homogenizing the crude ethosome in the step (3) to obtain the composite green pricklyash peel volatile oil nano ethosome.
Preferably, the mixing in step (1) is carried out at 25-30deg.C and 150-600rpm for 10-30min.
Preferably, the adding of the organic phase into the water in the step (2) is to drop the organic phase into the water at a constant speed through a peristaltic pump under stirring; more preferably, the stirring speed is 150-600rpm, and the dripping speed of the peristaltic pump is 1.0-5.0ml/min.
Preferably, the mixing in step (2) is carried out at 25-35℃and 150-600rpm for 10-30min.
Preferably, the homogeneous dispersion in the step (3) is a high-speed shearing homogeneous dispersion of 5000-9000rpm for 5-20min.
Preferably, the homogenization in the step (4) is carried out under high pressure, the pressure is 600-1200bar, and the cycle number is 2-6.
The application of the composite green pricklyash volatile oil nano-ethosome with antibacterial, anti-inflammatory and repairing effects in preparing cosmetics is also provided.
As a preferable scheme, the addition amount of the composite green pricklyash peel volatile oil nano-alcohol plastid with antibacterial, anti-inflammatory and repairing effects in the preparation of cosmetics is 0.5-10wt%.
Compared with the prior art, the embodiment of the invention has the following beneficial effects:
1. compared with the green pricklyash volatile oil, the composite green pricklyash volatile oil liposome and the composite green pricklyash volatile oil nanoemulsion, the composite green pricklyash volatile oil liposome has better transdermal absorption performance, antibacterial effect and anti-inflammatory repair effect, can be stably stored for 3 months in different environments, has better storage stability, and is mild and free of irritation.
2. The composite green pricklyash volatile oil ethosome is compounded by green pricklyash volatile oil, ceramide E and camphor tree extract, and the synergistic effect is achieved, so that the product is milder, and has better antibacterial, anti-inflammatory and repairing effects.
Drawings
FIG. 1 is the rate of change of particle size over 3 months for the samples of example 1 and comparative examples 1, 9, 10.
Fig. 2 is the rate of change of encapsulation efficiency over 3 months for the samples of example 1 and comparative examples 1, 9, 10.
FIG. 3 shows the particle diameter change rate of the samples of examples 1 to 3 and comparative example 5 in 3 months.
Fig. 4 shows the change rate of the encapsulation efficiency of the samples of examples 1 to 3 and comparative example 5 in 3 months.
FIG. 5 shows the particle diameter change rate of the samples of examples 1,4 to 8 in 3 months.
FIG. 6 is the percent change in encapsulation efficiency over 3 months for the samples of examples 1, 4-8.
FIG. 7 shows the particle diameter change rate of the samples of example 1, comparative examples 1 to 4, and comparative examples 6 to 10 in 3 months.
Fig. 8 is the encapsulation efficiency change rate of the samples of example 1, comparative examples 1 to 2, and comparative examples 6 to 10 over 3 months.
FIG. 9 shows the inhibition of TNF alpha before and after using the samples of example 1, comparative examples 1-12 and 2% green pricklyash volatile oil solution.
FIG. 10 shows the rate of change of skin moisture loss (as compared to the damage value) through skin at various time intervals before and after using the samples of example 1, comparative examples 1-12 and 2% green pricklyash peel volatile oil solution.
FIG. 11 is the rate of change of skin red pigment content (as compared to the damage value) for each period before and after using the samples of example 1, comparative examples 1-12 and 2% green pricklyash peel volatile oil solution.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples 1 to 6
Examples 1-6 respectively provide a composite green pricklyash peel volatile oil nanoalcohol body, the formula is shown in table 1, wherein the camphor tree extract is a mixture of camphor tree bark oil and camphor tree leaf extract, and the mass ratio of the camphor tree bark oil is as follows: camphor tree leaf extract = 1:1, the preparation method comprises the following steps:
(1) Mixing soybean lecithin, 1, 2-hexanediol, pericarpium Zanthoxyli volatile oil, ceramide E, and camphor tree extract at 30deg.C and 300rpm for 15min to obtain organic phase;
(2) Under the magnetic stirring of 300rpm, dripping the organic phase into deionized water at a constant speed of 2.0ml/min by a peristaltic pump, and stirring at 30 ℃ for 10min at 300rpm to obtain a mixed solution;
(3) Shearing, homogenizing and dispersing the mixed solution at 6000rpm for 10min to obtain crude ethosome;
(4) Homogenizing the crude ethosome under high pressure of 800bar, and circulating for 4 times to obtain composite pericarpium Zanthoxyli volatile oil nanometer ethosome.
Table 1 the formulation of the composite green pricklyash peel volatile oil nanoalcohol plastid of examples 1-6
| Group of | Soybean lecithin | 1, 2-hexanediol | Volatile oil of green pricklyash peel | Ceramide E | Camphor tree extract | Deionized water |
| Example 1 | 1 | 20 | 2 | 1 | 2 | 74 |
| Example 2 | 1 | 20 | 1 | 1 | 2 | 75 |
| Example 3 | 1 | 20 | 4 | 1 | 2 | 72 |
| Example 4 | 1 | 5 | 2 | 1 | 2 | 89 |
| Example 5 | 1 | 10 | 2 | 1 | 2 | 84 |
| Example 6 | 1 | 30 | 2 | 1 | 2 | 64 |
Examples 7 to 8
Examples 7 to 8 respectively provide a composite green pricklyash peel volatile oil nanoalcohol body, the preparation method is the same as that of example 1, the formula is basically the same as that of example 1, and the differences are as follows:
the small molecule polyol of example 7 was 1, 3-propanediol;
the small molecule polyol of example 8 is glycerol.
Example 9
The embodiment 9 provides a composite green pricklyash peel volatile oil nanoalcohol liposome, which comprises the following components in parts by mass: 1 part of hydrogenated lecithin, 20 parts of 1, 2-hexanediol, 2 parts of green pricklyash peel volatile oil, 1 part of ceramide E, 2 parts of camphor tree extract and 74 parts of deionized water, wherein the camphor tree extract is a mixture of camphor tree bark oil and camphor tree leaf extract, and the mass ratio of the camphor tree bark oil is as follows: camphor tree leaf extract = 1:1.
the preparation method comprises the following steps:
(1) Mixing hydrogenated lecithin, 1, 2-hexanediol, pericarpium Zanthoxyli volatile oil, ceramide E and camphor tree extract at 35deg.C and 300rpm for 20min to obtain organic phase;
(2) Under the magnetic stirring at 400rpm, dripping the organic phase into deionized water at a constant speed of 1.5ml/min by a peristaltic pump, and stirring at 35 ℃ for 10min at 200rpm to obtain a mixed solution;
(3) Homogenizing and dispersing the mixed solution at 8000rpm for 10min to obtain crude ethosome;
(4) Homogenizing the crude ethosome under 1000bar pressure, and circulating for 3 times to obtain composite pericarpium Zanthoxyli volatile oil nanometer ethosome.
Example 10
The embodiment 10 provides a composite green pricklyash peel volatile oil nanoalcohol liposome, which comprises the following components in parts by mass: 1 part of sphingomyelin, 20 parts of 1, 2-hexanediol, 2 parts of green pricklyash peel volatile oil, 1 part of ceramide E, 2 parts of camphor tree extract and 74 parts of deionized water, wherein the camphor tree extract is a mixture of camphor tree bark oil and camphor tree leaf extract, and the mass ratio of the camphor tree bark oil is as follows: camphor tree leaf extract = 1:1.
the preparation method comprises the following steps:
(1) Mixing sphingomyelin, 1, 2-hexanediol, pericarpium Zanthoxyli volatile oil, ceramide E and camphor tree extract at 25deg.C and 300rpm/ml for 15min to obtain organic phase;
(2) Under the magnetic stirring of 500rpm, dripping the organic phase into deionized water at a constant speed of 1.0ml/min by a peristaltic pump, and stirring at 30 ℃ and 200rpm for 20min to obtain a mixed solution;
(3) Shearing, homogenizing and dispersing the mixed solution at 6000rpm for 5min to obtain crude ethosome;
(4) Homogenizing the crude ethosome under high pressure of 600bar, and circulating for 6 times to obtain composite pericarpium Zanthoxyli volatile oil nanometer ethosome.
Example 11
The embodiment 11 provides a composite green pricklyash peel volatile oil nanoalcohol liposome, which comprises the following components in parts by mass: 5 parts of soybean lecithin, 30 parts of 1, 2-hexanediol, 4 parts of green pepper volatile oil, 2 parts of ceramide E, 5 parts of camphor tree extract and 54 parts of deionized water, wherein the camphor tree extract is a mixture of camphor tree bark oil and camphor tree leaf extract, and the mass ratio of the camphor tree bark oil is as follows: camphor tree leaf extract = 1:1.
the preparation method comprises the following steps:
(1) Stirring soybean lecithin, 1, 2-hexanediol, pericarpium Zanthoxyli volatile oil, ceramide E, and camphor tree extract at 30deg.C for 20min to obtain organic phase;
(2) Dripping the organic phase into deionized water at a constant speed of 2.0ml/min by a peristaltic pump under magnetic stirring at 150rpm, and stirring at 30deg.C and 500rpm for 10min to obtain a mixed solution;
(3) The mixed solution is sheared, homogenized and dispersed for 8min at a high speed of 7000rpm, and crude ethosome is obtained;
(4) Homogenizing the crude ethosome under high pressure of 800bar, and circulating for 3 times to obtain composite pericarpium Zanthoxyli volatile oil nanometer ethosome.
Comparative examples 1 to 8
Comparative examples 1-8 respectively provide composite green pricklyash peel volatile oil nanoalcohol bodies, the formula is shown in table 2, and the preparation method is the same as that of example 1.
Table 2 the formulation of composite green pricklyash peel volatile oil nanoalcohol plastid of comparative examples 1-8
| Group of | Soybean lecithin | 1, 2-hexanediol | Volatile oil of green pricklyash peel | Ceramide E | Camphor tree extract | Deionized water |
| Comparative example 1 | 1 | 20 | 2 | 0 | 0 | 77 |
| Comparative example 2 | 1 | 20 | 5 | 0 | 0 | 74 |
| Comparative example 3 | 1 | 20 | 0 | 5 | 0 | 74 |
| Comparative example 4 | 1 | 20 | 0 | 0 | 5 | 74 |
| Comparative example 5 | 1 | 20 | 5 | 1 | 2 | 71 |
| Comparative example6 | 1 | 20 | 2 | 1 | 0 | 76 |
| Comparative example 7 | 1 | 20 | 2 | 0 | 2 | 75 |
| Comparative example 8 | 1 | 20 | 0 | 1 | 2 | 76 |
Comparative example 9
Comparative example 9 provides a composite green pricklyash volatile oil liposome, which comprises the following components in parts by mass: 1 part of soybean lecithin, 0.1 part of cholesterol, 2 parts of green pricklyash peel volatile oil, 1 part of ceramide E, 2 parts of camphor tree extract and 93.9 parts of deionized water.
The preparation method comprises the following steps:
(1) Dissolving soybean lecithin, cholesterol, pericarpium Zanthoxyli volatile oil, ceramide E, and camphor tree extract in 30% ethanol, stirring at 60deg.C and 150rpm for 30min to obtain organic phase;
(2) The ethanol is removed by rotary evaporation of the organic phase, deionized water is added, 800w ultrasonic dispersion is carried out for 30min, and a 0.45 mu m organic microporous filter membrane is used for filtration, thus obtaining the composite green pricklyash peel volatile oil plastid.
Comparative example 10
Comparative example 10 provides a composite green pricklyash peel volatile oil nanoemulsion, which comprises the following components in parts by mass: 2 parts of green pricklyash peel volatile oil, 1 part of ceramide E, 2 parts of camphor tree extract, 15 parts of caprylic/capric triglyceride, 10 parts of 1, 2-hexanediol and 70 parts of deionized water.
The preparation method comprises the following steps:
(1) Mixing pericarpium Zanthoxyli volatile oil, ceramide E, camphor tree extract, caprylic/capric triglyceride at 80deg.C for 15min, and adding 1, 2-hexanediol to obtain oil phase;
(2) Dripping deionized water into the oil phase at a constant speed of 2.0ml/min by a peristaltic pump to obtain a mixed solution;
(3) Shearing the mixed solution in the step (2) at a high speed at 15000rpm, homogenizing at a high pressure of 800bar, and performing high-pressure homogenizing circulation for 4 times to obtain the composite green pricklyash peel volatile oil nanoemulsion.
Comparative examples 11 to 12
Comparative examples 11 to 12 respectively provide a composite pepper volatile oil nanoalcohol body, the preparation method is the same as that of example 1, the formula is basically the same as that of example 1, and the differences are as follows:
comparative example 11 green pricklyash peel volatile oil was changed to red pricklyash peel volatile oil;
comparative example 12 camphor tree extract was exchanged for rosewood oil.
Effect example 1 characterization of each example and comparative example
Object of measurement: samples of examples 1 to 11 and comparative examples 1 to 12.
Measurement items: particle size, particle size dispersion coefficient (PDI), and encapsulation efficiency.
Particle size and PDI were detected by a Markov Nano ZS90 particle size potential detector with a test angle of 90℃and a test temperature of 25℃and 3 parallel experiments were set for each group of experiments, and arithmetic average was taken and the experimental results are shown in Table 3.
Respectively taking 200 mu L of samples of examples 1-11, comparative examples 1,2 and 5-12, performing ultrafiltration and centrifugation at 9000rpm for 30min, and measuring the content of the pepper alkaloid in the filtrate so as to calibrate the content of the free pepper volatile oil which is not coated in the ethosome solution; taking an ethosome solution according to the volume ratio: methanol=1:9, methanol is added, ultrasonic demulsification is carried out for 30min, and after filtration through a 0.45 mu m organic phase filter membrane, the content of the zanthoxylum alkaloids is measured, so that the total content of the zanthoxylum volatile oil in the ethosome solution is calibrated. Measurement of the alkaloid content of Zanthoxylum bungeanum reference: li Yang near infrared spectrum nondestructive test of alkaloid and volatile oil content of fructus Zanthoxyli [ D ]. University of North Western agriculture and forestry, 2012,13-14.
Encapsulation Efficiency (EE) was calculated according to the following formula, the results are shown in table 3:
wherein C is 1 The concentration of the free pepper volatile oil which is not coated in the ethosome solution; c (C) 0 The total concentration of the pricklyash peel volatile oil in the ethosome solution after being demulsified by methanol ultrasonic.
TABLE 3 characterization data for samples of examples 1-11, comparative examples 1-12
The composite green pricklyash volatile oil nanobody of example 1, the green pricklyash volatile oil nanobody of comparative example 1, the liposome of comparative example 9, and the nanoemulsion of comparative example 10 were analyzed. The smaller particle size and PDI of example 1 and higher encapsulation efficiency compared to comparative example 1, demonstrate that nanool bodies added with ceramide E and camphor tree extract have smaller particle size, narrower particle size distribution, and higher encapsulation efficiency. The particle size, PDI less, encapsulation efficiency greater than the comparative example 8, comparative example 9 samples of example 1 demonstrate that inclusions made with nanoethosome dosage forms have smaller particle size, narrower particle size distribution, and higher encapsulation efficiency than liposomes and nanoemulsions. The smaller the particle diameter of the nanocarrier, the more easily the transdermal absorption performance of the nanocarrier is exhibited. Therefore, compared with the green pricklyash volatile oil nanoalcohol body, the composite green pricklyash volatile oil nanoalcohol body, the liposome and the nanoemulsion, the composite green pricklyash volatile oil nanoalcohol body has more excellent transdermal absorption performance.
From the analysis of example 1 and comparative examples 11 and 12, it is clear from table 3 that the particle size, PDI and encapsulation efficiency of example 1 and comparative example 11 are smaller, and the particle size, PDI and encapsulation efficiency of example 1 are better than those of comparative example 12, which means that the encapsulation efficiency of the ethosome is not greatly affected by the replacement of the zanthoxylum schinifolium volatile oil with the safflower pepper volatile oil, but the encapsulation efficiency of the ethosome is reduced by the replacement of the camphor tree extract with the rosewood oil, so that the encapsulation efficiency can be improved by adding the camphor tree extract into the nano-ethosome for preparing the composite zanthoxylum schinifolium volatile oil.
The composite green pricklyash volatile oil nanoalcohol plastids of examples 1-3 and comparative example 5 were analyzed. Examples 1-3 differ from comparative example 5 in the amount of the included green pricklyash volatile oil. The particle size of the composite green pricklyash volatile oil nanoalcohol plastid of comparative example 3 is larger than that of examples 1-3, and the encapsulation efficiency is lower than that of examples 1-3. Therefore, in the invention, in order to prepare the composite green pricklyash peel volatile oil nano-ethosome with smaller particle size and higher encapsulation efficiency, the green pricklyash peel volatile oil should be set to 0.5-4 parts.
The composite green pricklyash peel volatile oil nanoalcohol plastids of the example 1 and the examples 4 to 11 are analyzed. The composite green pricklyash peel volatile oil nanoalcohol bodies of the embodiment 1 and the embodiment 4 to 6 and 11 are different in the use amount of the components; the composite green pricklyash peel volatile oil nanoalcohol bodies in examples 1, 7 and 8 are different in the types of small-molecule polyols used; the composite green pricklyash volatile oil nanoalcohol bodies in examples 1, 9 and 10 are different in the types and amounts of phospholipids. As can be seen from Table 3, the composite green pricklyash peel volatile oil nanoalcohol body of example 1 has the smallest particle size and the highest encapsulation efficiency, and the components and the amounts thereof of example 1 are the most preferable. The particle size of the samples in examples 1 and 4-6 is increased along with the increase of the addition amount of 1, 2-hexanediol, the particle size of the composite green pricklyash peel volatile oil nanoalcohol body is firstly reduced, then the particle size is increased, the encapsulation rate is firstly increased, then the particle size is reduced, the particle size is minimum when the use amount of 1, 2-hexanediol is 20 parts by mass, and the encapsulation rate is highest, so that the use amount of polyol molecules in the composite green pricklyash peel volatile oil nanoalcohol body is preferably 20 parts by mass. The samples of examples 7 and 8 with larger particle size and lower encapsulation efficiency than the sample of example 1 show that the more the number of carbon in the molecular structure of the polyol, the more favorable the preparation of the composite green pricklyash peel volatile oil nanoalcohol bodies. The samples of examples 9 and 10 have larger particle sizes and lower encapsulation efficiency than the sample of example 1, which indicates that the soybean lecithin in the phospholipid can better reduce the particle size of the sample and improve the encapsulation efficiency. The sample with the particle size larger than that of the sample in the example 11 and the encapsulation efficiency lower than that of the sample in the example 1 shows that the optimal dosage of each component of the composite green pricklyash peel volatile oil nanoalcohol liposome is 1 part of phospholipid, 20 parts of small molecular polyalcohol, 2 parts of green pricklyash peel volatile oil, 1 part of ceramide E, 2 parts of camphor tree extract and 74 parts of water.
Effect example 2 measurement of storage stability
600mL of the sample was taken and dispensed into 6 colorless and transparent sample bottles, each bottle having 100mL. The 6 sample bottles are respectively placed in different storage environments, the changes of the appearance, the particle size and the encapsulation efficiency of the samples in the 6 sample bottles in the different storage environments are measured, the storage stability of the samples is examined, the results of examples are shown in Table 4, the results of comparative examples are shown in Table 5, and the changes of the particle sizes and the encapsulation efficiencies of the examples and the comparative examples are shown in FIGS. 1-8.
Object of measurement: samples of examples 1 to 11 and comparative examples 1 to 12.
Storage environment: -18 ℃,4 ℃, light shielding at room temperature, illumination at room temperature, high-low temperature alternating circulation and 45 ℃.
Sample state investigation time point: initialization state, storage for 1 month, 2 months and 3 months.
The absolute values of the particle diameter change rate and the encapsulation rate change rate of the samples stored for 3 months were calculated from the particle diameter and the encapsulation rate at each time point, and the calculation formula was as follows:
wherein n represents 1,2 or 3.
Wherein n represents 1,2 or 3.
TABLE 4 results of storage stability test of samples of examples 1 to 11
TABLE 5 results of storage stability test for samples of comparative examples 1 to 12
As can be seen from tables 4, 5 and fig. 1 and 2, the absolute values of the particle size change rate and the encapsulation rate change rate of example 1 in 3 months are smaller than those of comparative examples 1, 9 and 10, and the results show that the composite green pricklyash volatile oil nanoalcohol plastid has better stability than the composite green pricklyash volatile oil liposome and the composite green pricklyash volatile oil nanoemulsion.
According to table 4, fig. 3 and fig. 4, the absolute values of the particle size change rate and the encapsulation rate change rate of the composite green pricklyash volatile oil nanoalcohol bodies of examples 1-3 within 3 months are smaller than those of the composite green pricklyash volatile oil nanoalcohol body of comparative example 5. Therefore, the addition amount of the green pricklyash volatile oil can influence the particle size, encapsulation efficiency and stability of the composite green pricklyash volatile oil nanoalcohol plastid. When the adding amount of the green pricklyash volatile oil exceeds 4 parts, the encapsulation rate of the composite green pricklyash volatile oil nano-alcohol plastid on the green pricklyash volatile oil is reduced, and after a period of time, the nano-alcohol plastid is easy to agglomerate, and the particle size of the nano-alcohol plastid is obviously increased.
As can be seen from table 4 and fig. 5 to 8, the absolute value of the change rate of the particle diameter of example 1 is smaller than that of examples 4 to 8, comparative examples 1 to 4, and comparative examples 6 to 8, and the absolute value of the change rate of the encapsulation efficiency of example 1 is smaller than that of examples 4 to 8, comparative examples 1 to 2, and comparative examples 6 to 8. The result of the combination effect example 1 shows that the particle size, encapsulation efficiency and stability of the composite green pricklyash volatile oil nanoalcohol body are all affected by the types and the amounts of the components, so the types and the amounts of the components in the example 1 are most preferable. The absolute values of the particle diameter change rates of example 1 are smaller than those of comparative examples 6 to 10, and although the encapsulation rate change rates are not quite different from those of examples, the particle diameters are too large to meet the object of the present invention. The particle size and encapsulation efficiency change rate of example 1 are similar to those of comparative example 11, and are superior to those of comparative example 12, and the result of the combination effect example 1 can be obtained, the green pricklyash volatile oil in the composite green pricklyash volatile oil nano-alcoholic body has little influence on the encapsulation efficiency stability of the alcoholic body, but the camphor tree extract is replaced by rosewood oil, so that the encapsulation efficiency stability of the alcoholic body is reduced.
Effect example 3 antibacterial test
Test object: samples of example 1 and comparative examples 1 to 12.
Test conditions: activating Escherichia coli strain at 1.0X10 5 CFU/mL is the initial concentration, 20mL bacterial liquid is taken for plating. Activating Staphylococcus aureus strain at a ratio of 5×10 7 CFU/mL is the initial concentration, 20mL bacterial liquid is taken for plating. And respectively adding 10 mu L of the sample stock solutions into the escherichia coli and staphylococcus aureus bacterial solutions, uniformly mixing, culturing for 24 hours, and calculating the colony number of the viable bacteria. The above procedure was repeated with 1 XPBS instead of the test sample as a control sample. The test results are expressed in terms of bacteriostasis rate, the results are shown in Table 6, and the bacteriostasis rate has the following calculation formula:
antibacterial ratio (%) = [ (average colony count of control sample-average colony count of test sample)/average colony count of control sample ] ×100%
TABLE 6 antibacterial Rate of samples of example 1, comparative examples 1 to 12
According to the results of Table 6, the composite green pricklyash peel volatile oil nanoalcohol bodies of example 1, which are only added with green pricklyash peel volatile oil, ceramide E and camphor tree extract except lipid and small molecular polyalcohol, have better antibacterial effect compared with comparative examples 1-4 and 6-8, which shows that the green pricklyash peel volatile oil, ceramide E and camphor tree extract can play a synergistic effect of antibacterial effect. The antibacterial rates of the liposome of comparative example 9 and the nanoemulsion of comparative example 10 on escherichia coli are lower than those of the composite green pricklyash volatile oil nanoalcohol liposome of example 1, which shows that the antibacterial effect of the composite green pricklyash volatile oil nanoalcohol liposome is better than that of the liposome and the alcohol liposome. The bacteriostasis rate of the nano-ethosome of comparative example 11 on escherichia coli and staphylococcus aureus is lower than that of the nano-ethosome of the composite green pricklyash volatile oil of example 1, which shows that the bacteriostasis effect of the nano-ethosome of the composite green pricklyash volatile oil is better than that of the nano-ethosome of the composite red pricklyash volatile oil. The bacteriostasis rate of the nano-ethosome of comparative example 12 on escherichia coli and staphylococcus aureus is lower than that of the nano-ethosome of the composite green pricklyash volatile oil of example 1, which shows that the bacteriostasis effect of the ethosome obtained by compounding the green pricklyash volatile oil and the camphor tree extract is better.
Effect example 5 skin irritation test
Test object: samples of example 1, comparative example 2, comparative examples 9-12, 2% solution of green pricklyash peel volatile oil.
The testing method comprises the following steps: skin irritation detection is carried out on white rabbits according to cosmetic safety technical Specification (2015 edition), 4 rabbits are used as each sample, the test part is the fur on two sides of the spine of the back of the rabbits, the dehairing range is 3X 3cm, 0.5mL of the sample is smeared on the skin on one side, the smearing area is 2.5X 2.5cm, the other side is not treated, the smearing is carried out once a day for 14 days, the dehairing treatment is carried out on the test area before each smearing from the next day of smearing, the residual test object is clearly remained by pure water, and the irritation of the sample is inspected according to the skin irritation response scoring table and the skin irritation intensity classification of the cosmetic safety technical Specification (2015 edition) after the smearing for 1 hour, and the inspection result is shown in Table 7.
Samples of examples and comparative examples were pre-treated prior to testing and diluted to 10% solution with deionized water.
TABLE 7 results of irritation test of samples of example 1, comparative examples 1,2, 9-12 and 2% green pricklyash peel volatile oil
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According to the results of Table 7, the composite green pricklyash peel volatile oil nanoalcohol plastid of example 1 shows no irritation and the composite green pricklyash peel volatile oil nanoalcohol plastid of comparative example 1 shows light irritation in skin irritation test, which shows that the addition of ceramide E and camphor tree extract can properly reduce the irritation of the composite green pricklyash peel volatile oil nanoalcohol plastid to skin, so that the product is milder. The liposome of the comparative example 9 and the nanoemulsion of the comparative example 10 both show light irritation, and the 2% green pricklyash peel volatile oil sample shows medium irritation, which shows that compared with the liposome and nanoemulsion, the nanoliposome encapsulation mode can effectively reduce the irritation of the composite green pricklyash peel volatile oil to the skin, so that the product is mild and has no irritation. The composite red pepper volatile oil nanoalcohol plastid of comparative example 11 shows no irritation, which shows that the system can reduce the irritation of the red pepper volatile oil to the skin; the composite green pricklyash peel volatile oil nanoalcohol plastid of comparative example 12 shows light irritation, which indicates that the irritation of green pricklyash peel volatile oil can be further reduced by adding camphor tree extract into the composite green pricklyash peel volatile oil nanoalcohol plastid system. Therefore, the nanometer ethosome wrapping technology is adopted, and the ceramide E and camphor tree extract are combined to act together to reduce the skin irritation caused by the green pricklyash peel volatile oil, so that the composite green pricklyash peel volatile oil nanometer ethosome disclosed by the embodiment 1 has an optimal effect of reducing the skin irritation.
Effect example 5 anti-inflammatory test
Test object: samples of example 1 and comparative examples 1 to 12 were 2% green pricklyash peel volatile oil solution.
Test items: cytotoxicity and cellular tnfα inhibition.
The materials or reagent sources used in the following experiments are described as follows:
mouse macrophage Raw 264.7, purchased from kunming cell bank of the national academy of sciences;
TNFα ELISA Kit, available from Shanghai Biyunshan Biotechnology Co., ltd., mouse TNFα ELISA Kit;
the other materials are commercially available without any particular explanation.
(1) Cytotoxicity test
The toxicity of test samples at different concentrations to mouse macrophages was determined using MTT staining.
Cytotoxicity test was performed using mouse macrophage row 264.7, cell lines in 10% foetal calf serum high sugar DMEM medium at 37deg.C, 5% CO 2 Culturing under saturated humidity condition. The following medium (liquid) is a high-sugar DMEM medium with 10% fetal bovine serum, unless otherwise specified; cell incubations were all 37℃and 5% CO without any particular indication 2 And under saturated humidity conditions.
The samples were pre-treated and diluted by the medium to 1.25%, 2.5%, 5% and 10% strength solutions, respectively. And (3) injection: the 2% green pricklyash peel volatile oil solution is taken as a whole sample and diluted with a culture medium, and the 4 concentrations are not the final concentration of the green pricklyash peel volatile oil.
Preparing cell suspension by using culture medium, inoculating 100 μl of cell suspension into 96-well cell culture plate with cell number of 3×10 4 After incubation for 24h, kong Zhongyuan medium was discarded, 100 μl of test sample was added to each well, after incubation in an incubator for 24h, 100 μl of 0.1mg/mL MTT solution was added to each well, after incubation for 4h, the liquid in the wells was removed, 150 μl DMSO was added to each well, and after shaking for 15min, absorbance was measured by an enzyme-labeled instrument at 570nm wavelength. The control group replaced the test samples with 100 μl of culture broth, each sample being set in 3 replicates. The relative cell activities of the groups were calculated with the cell activity of the control group as 100%, the results are shown in Table 8, the concentration of the cell activity of 90% or more is used as the highest safe concentration of macrophages, and the anti-inflammatory test concentration should not be selected in the rangeExceeding the highest safe concentration.
TABLE 8 cytotoxicity test results of example 1, samples of comparative examples 1-12 and 2% green pricklyash peel volatile oil
As can be seen from the results of table 8, samples of example 1 and comparative examples 1 to 12 and 2% of the green pricklyash peel volatile oil were in a concentration range of 5%, and cells were not significantly cytotoxic, so the concentration of the sample for tnfα content measurement was selected to be 3%.
(2) TNFa inhibition assay
Establishing a verification model by stimulating macrophages in vitro by LPS, measuring the TNFα content of cells by an enzyme-linked immunosorbent assay method, calculating the TNFα inhibition rate, and carrying out the specific steps of TNFα content measurement according to the specification of a kit, wherein the cell strain and the culture method are the same as those of cytotoxicity tests.
The sample was subjected to pretreatment, and the sample was diluted to a 3% strength solution by the culture solution. And (3) injection: the 2% green pricklyash peel volatile oil solution is taken as a whole sample and diluted with a culture medium, and the 4 concentrations are not the final concentration of the green pricklyash peel volatile oil.
The experiment was performed in a total of 4 groups, namely, an experimental group, a negative control group (blank) and a modeling group (LPS group). Adding a sample and lipopolysaccharide to the experimental group; the negative control group was supplemented with only culture medium; the modeling group added culture broth and lipopolysaccharide.
After the macrophage is incubated for 24 hours in a 96-well cell culture plate, the culture solution in the well is discarded, 100 mu L of sample solution is added into each well of an experimental group, 200 mu L of culture solution is added into each well of a negative control group and a positive control group respectively, 10ng/mL of lipopolysaccharide is added into each well of the experimental group and the positive control group respectively after incubation for 2 hours, after incubation for 24 hours, the culture solution in the well is taken for centrifugation to keep supernatant, the content of TNF alpha in the supernatant is measured, the relative content of TNF alpha is set to 100 by taking the TNF alpha content of an LPS group as a standard, the relative contents of the groups of the examples and the comparative examples are calculated, the inhibition rate of the formation of the TNF alpha of the examples and the comparative examples is calculated according to the following formula, and the result of the TNF alpha inhibition rate is shown in figure 9.
Tnfα inhibition = (tnfα relative content of LPS group-tnfα relative content of experimental group) ×100%
The anti-inflammatory test results of the samples of example 1 and comparative examples 1 to 12 and 2% of the green pricklyash volatile oil are shown in fig. 9. The inhibition ratios of the samples of example 1, comparative examples 1 to 12 and the sample of the 2% green pepper volatile oil solution to the production of inflammatory factor tnfα were 71.8% (example 1), 51.7% (comparative example 1), 62.0% (comparative example 2), 25.3% (comparative example 3), 42.9% (comparative example 4), 65.9% (comparative example 5), 52.7% (comparative example 6), 48.3% (comparative example 7), 28.2% (comparative example 8), 44.9% (comparative example 9), 37.6% (comparative example 10), 49.7% (comparative example 11), 57.8% (comparative example 12), 44.7% (2% green pepper volatile oil solution), respectively, which indicates that the inhibition effect of example 1 to the production of inflammatory factor tnfα is superior to those of the comparative examples 1 to 12 and the 2% green pepper volatile oil solution. Therefore, the above results can demonstrate that the composite green pricklyash volatile oil nanoalcohol bodies of example 1 have optimal anti-inflammatory effects compared to comparative examples 1-12 and 2% green pricklyash volatile oil.
Effect example 6 human body repair efficacy test
Test object: the emulsion prepared by the samples of example 1 and comparative examples 1-12 and the 2% green pricklyash peel volatile oil solution respectively has the formula shown in table 9, and the preparation method comprises the following steps:
(1) Mixing a test sample, synthesized squalane, neopentyl glycol di (ethyl hexanoate), vitamin E and camellia seed oil, and heating to a liquid state at 90 ℃ to obtain a phase A;
(2) Uniformly mixing imidazolidinyl urea and water to obtain a phase B;
(3) Mixing glycerol, methylparaben, EDTA disodium, carbomer, arginine and water to obtain phase C;
(4) And (3) when the A phase is stirred and cooled to 45 ℃, adding the B phase, uniformly mixing, adding the C phase, and uniformly mixing to obtain the emulsion.
Table 9 sample emulsion formulations
The number of human repair efficacy test volunteers is 30, the age is 18-40 years, the human body is healthy, no skin care, wound, abrasion, temperature rise, pimple and other conditions which influence the result judgment and possibly influence the test result exist at the test part in one month, and 15 volunteers are arranged for each sample to test.
The two arms of each volunteer are provided with 5 sample test areas and 1 blank control area (3 areas are divided on the inner side of each forearm), and the samples and the blank control areas are distributed on the inner side area of the forearm of the volunteer according to a random distribution table of the test areas. Repeatedly sticking an adhesive tape of 3X 3cm for 8 times in a test area to establish a physical damage model, dipping 0.0172-0.0189g of sample emulsion by using a disposable medical cotton swab, uniformly smearing the sample emulsion back and forth in the test area for 5 times, repeatedly smearing the sample solution by using a clean cotton swab for 1 time, and measuring skin percutaneous moisture loss and skin pigment of the area in different periods without smearing any sample in a blank control area.
Skin percutaneous moisture loss was detected in the test areas by a three-probe skin moisture loss test probe tewatter 330T, each area was measured 1 time for 30 seconds, and the average value of 20 seconds was taken as the measurement value.
The skin heme content of the test area is detected by a skin pigment test probe Mexameter MX18, each area is detected for 3 times, and the average value of the detected values of 3 times is taken as a measured value.
The time points of skin percutaneous moisture loss and skin pigment detection are before the tape injury (original value, marked as D0), after the tape injury (injury value, marked as D1), after 2 days of using the emulsion (2 days of repairing, marked as D3), after 4 days of using the emulsion (4 days of repairing, marked as D5), and after 6 days of using the emulsion (6 days of repairing, marked as D7).
The average value, the difference value and the change rate are calculated according to the skin percutaneous water loss measured value, the average value result is shown in table 10, and the calculation formulas of the average value, the difference value and the change rate are as follows:
wherein x is n Represents the individual parameter detection value, and n represents the effective data amount.
Wherein,mean value of parameters at the time of detection at the nth time point after use, ±>Mean values of parameters detected before use are shown.
Wherein,the average value of the parameter detected before use is shown, and the change rate shows the average change degree relative to the initial value.
The average value, the difference value and the change rate are calculated according to the measured value of the skin red content, the average value result is shown in table 11, and the calculation formula is the same as the measured value of the skin percutaneous water loss.
TABLE 10 measurement results (average value) of skin moisture loss of emulsion for each sample
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As can be seen from table 10, the skin moisture loss of D7 was reduced by comparing D7 (6 days of repair) with D1 (damage value) of each group except for the blank, indicating that the skin moisture loss was improved to some extent after using the example 1, comparative examples 1 to 12 and the 2% green pepper volatile oil samples corresponding to the emulsions. As can be seen from fig. 11, in the D7 stage, the skin percutaneous moisture loss change rates were-14.5% (example 1), -10.0% (comparative example 1), -10.9% (comparative example 2), -11.4% (comparative example 3), -8.6% (comparative example 4), -11.2% (comparative example 5), -10.1% (comparative example 6), -11.0% (comparative example 7), -6.8% (comparative example 8), -9.0% (comparative example 9), -7.5% (comparative example 10), -9.4% (comparative example 11), -5.2% (comparative example 12), -8.2% (2% green pricklyash peel volatile oil solution), respectively, after using the examples 1, comparative example 1-12 and the 2% green pricklyash peel volatile oil corresponding emulsion samples, to demonstrate that the composite green pricklyash peel volatile oil nanoliposome of example 1 is most effective in improving skin percutaneous moisture loss, compared to the examples 1-12 and the 2% green pricklyash peel volatile oil samples.
TABLE 11 determination of skin Red pigment content of sample emulsions (average value)
As can be seen from table 11, D7 (6 days of repair) was compared to D1 (damage value) for each group except for the blank, and the skin red pigment content of D7 was reduced, indicating that skin inflammation was improved using the example 1, comparative examples 1-12 samples and 2% green pepper volatile oil corresponding emulsion. In the D7 stage, the skin red pigment content change rates of the samples of the example 1, the comparative examples 1-12 and the 2% green pricklyash peel volatile oil samples are respectively-18.8% (example 1), -10.2% (comparative example 1), -12.3% (comparative example 2), -9.7% (comparative example 3), -8.2% (comparative example 4), -13.1% (comparative example 5), -8.1% (comparative example 6), -8.8% (comparative example 7), -7.3% (comparative example 8), -10.6% (comparative example 9), -10.1% (comparative example 10), -6.9% (comparative example 11), -10.6% (comparative example 12), -7.0% (2% green pricklyash peel volatile oil sample), so that the composite green pricklyash peel volatile oil nanobody of the example 1 has the best effect on reducing the skin red pigment content.
In conclusion, the composite green pricklyash peel volatile oil nano-alcohol plastid can effectively improve the percutaneous moisture loss and the skin red pigment content of human skin, achieves the effects of resisting bacteria, resisting inflammation and repairing, has excellent transdermal absorption performance, and has good storage stability, mildness and no stimulation.
The foregoing embodiments have been provided for the purpose of illustrating the general principles of the present invention, and are not to be construed as limiting the scope of the invention. It should be noted that any modifications, equivalent substitutions, improvements, etc. made by those skilled in the art without departing from the spirit and principles of the present invention are intended to be included in the scope of the present invention.
Claims (10)
1. The composite green pricklyash volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects is characterized by comprising the following components in parts by mass: 0.5-5 parts of phospholipid, 5-30 parts of micromolecular polyalcohol, 0.5-4 parts of green pepper volatile oil, 0.5-2 parts of ceramide E, 0.5-5 parts of camphor tree extract and water to make up to 100 parts of total mass; the small molecule polyalcohol comprises one or more of 1, 3-propanediol, glycerol, 1, 2-butanediol, pentanediol and 1, 2-hexanediol; the camphor tree extract is a mixture of camphor tree bark oil and camphor tree leaf extract, and the mass ratio of the camphor tree bark oil is as follows: camphor tree leaf extract = 1-5:1.
2. The composite green pricklyash volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects as claimed in claim 1, which is characterized by comprising the following components in parts by mass: 0.5-2 parts of phospholipid, 10-25 parts of micromolecular polyalcohol, 1-3 parts of green pricklyash peel volatile oil, 0.5-1.5 parts of ceramide E, 1-3 parts of camphor tree extract and water to make up to 100 parts of total mass.
3. The composite green pricklyash volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects as claimed in claim 2, which is characterized by comprising the following components in parts by mass: 1 part of phospholipid, 20 parts of small molecular polyalcohol, 2 parts of green pepper volatile oil, 1 part of ceramide E, 2 parts of camphor tree extract and 74 parts of water.
4. The composite green pricklyash volatile oil nanoliposome with antibacterial, anti-inflammatory and repairing effects according to any one of claims 1-3, wherein the phospholipid comprises one or more of soybean lecithin, hydrogenated lecithin, sphingomyelin and phosphatidylcholine.
5. The composite green pricklyash volatile oil nanoalcohol body with antibacterial, anti-inflammatory and repairing effects as claimed in claim 1, wherein the mixture mass ratio of camphor tree bark oil and camphor tree leaf extract is that of camphor tree bark oil: camphor tree leaf extract = 1:1.
6. The method for preparing the composite green pricklyash peel volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects according to any one of claims 1 to 5, which is characterized by comprising the following steps:
(1) Mixing phospholipid, small molecular polyalcohol, pericarpium Zanthoxyli volatile oil, ceramide E and camphor tree extract to obtain organic phase;
(2) Adding the organic phase in the step (1) into water, and mixing to obtain a mixed solution;
(3) Homogenizing and dispersing the mixed solution obtained in the step (2) to obtain crude ethosome;
(4) Homogenizing the crude ethosome in the step (3) to obtain the composite green pricklyash peel volatile oil nano ethosome.
7. The method for preparing the composite green pricklyash peel volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects according to claim 6, wherein the method comprises at least one of the following steps:
mixing in the step (1) at 25-30 ℃ and 150-600rpm for 10-30min;
adding the organic phase into the water in the step (2) to drop the organic phase into the water at a constant speed through a peristaltic pump under stirring;
the mixture in the step (2) is stirred for 10 to 30 minutes at the temperature of 25 to 35 ℃ and the rpm of 150 to 600 rpm;
the homogeneous dispersion in the step (3) is 5000-9000rpm high-speed shearing homogeneous dispersion for 5-20min;
the homogenization in the step (4) is high-pressure homogenization, the homogenization pressure is 600-1200bar, and the cycle times are 2-6 times.
8. The method for preparing the composite green pricklyash peel volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects according to claim 7, wherein the method comprises at least one of the following steps:
the peristaltic pump dropping speed of adding the organic phase into the water in the step (2) is 1.0-5.0ml/min;
the stirring speed is 150-600rpm.
9. The use of the composite green pricklyash volatile oil nanoalcohol plastid with antibacterial, anti-inflammatory and repairing effects according to any one of claims 1-5 in the preparation of cosmetics.
10. The composite green pricklyash volatile oil nanoalcohol liposome with antibacterial, anti-inflammatory and repairing effects as claimed in claim 9, wherein the addition amount of the composite green pricklyash volatile oil nanoalcohol liposome in the preparation of cosmetics is 0.5-10wt%.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2009234938A (en) * | 2008-03-26 | 2009-10-15 | Toyobo Co Ltd | Cosmetic composition containing vegetable origin polyamine-containing extract |
| JP2010018558A (en) * | 2008-07-11 | 2010-01-28 | Toyobo Co Ltd | Cosmetic composition containing biosurfactant |
| JP2014114290A (en) * | 2013-12-13 | 2014-06-26 | Showa Denko Kk | Cosmetic and skin external preparation |
| CN113475540A (en) * | 2021-07-22 | 2021-10-08 | 青岛众祥环保科技有限公司 | Preparation method and application of green pricklyash peel mite-killing spray with mite-killing effect |
| CN114177109A (en) * | 2021-12-17 | 2022-03-15 | 广州集妍化妆品科技有限公司 | Composite ceramide nano composition and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009234938A (en) * | 2008-03-26 | 2009-10-15 | Toyobo Co Ltd | Cosmetic composition containing vegetable origin polyamine-containing extract |
| JP2010018558A (en) * | 2008-07-11 | 2010-01-28 | Toyobo Co Ltd | Cosmetic composition containing biosurfactant |
| JP2014114290A (en) * | 2013-12-13 | 2014-06-26 | Showa Denko Kk | Cosmetic and skin external preparation |
| CN113475540A (en) * | 2021-07-22 | 2021-10-08 | 青岛众祥环保科技有限公司 | Preparation method and application of green pricklyash peel mite-killing spray with mite-killing effect |
| CN114177109A (en) * | 2021-12-17 | 2022-03-15 | 广州集妍化妆品科技有限公司 | Composite ceramide nano composition and preparation method and application thereof |
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