CN116369328B - Mite-killing composition containing azocyclotin and application thereof - Google Patents

Mite-killing composition containing azocyclotin and application thereof Download PDF

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CN116369328B
CN116369328B CN202310422955.5A CN202310422955A CN116369328B CN 116369328 B CN116369328 B CN 116369328B CN 202310422955 A CN202310422955 A CN 202310422955A CN 116369328 B CN116369328 B CN 116369328B
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azocyclotin
acaricidal composition
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mites
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CN116369328A (en
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王伟香
张永芳
刘金玲
王均竹
董成武
魏存
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Hailir Pesticides and Chemicals Group Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
    • A01N55/02—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms
    • A01N55/04—Tin
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
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  • Engineering & Computer Science (AREA)
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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Agronomy & Crop Science (AREA)
  • Insects & Arthropods (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of pesticide mite killing, and discloses a mite killing composition containing azocyclotin, which comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is pyflubumide, the active ingredient B is azocyclotin, and the mass ratio of the active ingredient A to the active ingredient B is 1:45-50:1. The acaricidal composition has obvious synergism on plant mites, particularly has obvious control effect on citrus panonychus, and has high safety on crops and no phytotoxicity.

Description

Mite-killing composition containing azocyclotin and application thereof
Technical Field
The invention belongs to the technical field of pesticide mite killing, and discloses a mite killing composition containing azocyclotin and application thereof.
Background
The panonychus citri is an important pest mite in citrus orchards in China and is also one of the most important pest mites reported in almost all citrus planting areas in the world. The mite is a multi-feeding spider mite. It can harm not only citrus but also pear, apple, peach and other plants. The citrus panonychus citri becomes mites and the mites eat nutrient organs such as buds, tender tips, leaves and the like of host plants, absorb juice of the citrus panonychus citri, cause the fruit tree vigor to decline, and influence the fruit yield.
Azocyclotin belongs to an organotin acaricide, is a broad-spectrum organotin acaricide, has strong contact killing property and long residual period, has very high activity on young mites, adult mites and eggs of spider mites, rust mites and the like, and can be used for preventing and controlling pest mites of crops such as citrus, apples, hawthorns, cotton, vegetables and the like. And azocyclotin can cause a certain degree of phytotoxicity to young leaves of new tips when being applied to spring tips and flowering periods of oranges, and in actual production, the phytotoxicity of oranges at different degrees is often caused due to improper pesticide use of oranges, so that great loss is caused to the production of oranges.
According to the invention, through indoor and field efficacy test researches, the inventor of the invention finds that azocyclotin and pyflubumide are reasonably compounded, obvious synergistic effect is shown on citrus panonychus in a certain range, and almost no phytotoxicity is generated on target crops such as citrus.
Disclosure of Invention
Based on the above circumstances, the invention aims to provide the azocyclotin-containing acaricidal composition and the application thereof, and the acaricidal composition has excellent control effect on plant mites, particularly has remarkable control effect on citrus panonychus, has long lasting period, is safe to crops and has no phytotoxicity.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the mite-killing composition containing azocyclotin is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is pyflubumide, the active ingredient B is azocyclotin, and the mass ratio of the active ingredient A to the active ingredient B is 1:45-50:1;
Further, the mass ratio of the active ingredient A to the active ingredient B is 1:45, 1:40, 1:35, 1:25, 1:10, 1:6, 1:1, 5:1, 15:1, 20:1, 30:1, 40:1, 50:1;
further, the mass ratio of the active ingredient A to the active ingredient B is 1:35-30:1;
further, the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:25, 1:10, 1:6, 1:1, 5:1, 15:1, 20:1, 30:1;
further, the mass ratio of the active ingredient A to the active ingredient B is 1:25-20:1;
further, the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:10, 1:6, 1:1, 5:1, 15:1 and 20:1;
Further, the total weight of the acaricidal composition is calculated by 100 weight percent, and the total weight of the active ingredient A and the active ingredient B accounts for 2-60% of the total weight of the acaricidal composition;
further, the total weight of the acaricidal composition is calculated by 100 weight percent, and the total weight of the active ingredient A and the active ingredient B accounts for 4-50% of the total weight of the acaricidal composition;
Further, the acaricidal composition comprises agriculturally acceptable auxiliary ingredients besides the active ingredients, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers;
Further, the wetting agent is selected from one or a mixture of more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrating agent F, chinese honeylocust fruit powder, silkworm excrement or soapberry powder;
Further, the dispersing agent is selected from one or more of polycarboxylate, lignosulfonate, alkylphenol ethoxylate formaldehyde condensate sulfate, calcium alkylbenzenesulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, alkylphenol ethoxylate, fatty amine ethoxylate, fatty acid ethoxylate or glycerin fatty acid ester ethoxylate;
Further, the emulsifier is selected from one or a mixture of more of calcium alkylbenzenesulfonate, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), phenylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether disulfonate magnesium salt, triethanolamine salt, benzyl dimethyl phenol polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether or fatty alcohol polyoxyethylene ether;
Further, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;
further, the disintegrating agent is selected from one or a mixture of more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;
further, the antifreezing agent is selected from one or a mixture of more of ethylene glycol, propylene glycol, glycerol or urea;
Further, the defoamer is selected from one or a mixture of more of silicone oil, silicone compounds, C 10～C20 saturated fatty acid compounds or C 8～C10 fatty alcohol compounds;
further, the solvent is selected from one or more of N, N-dimethylformamide, cyclohexanone, butyl ether, dimethylbenzene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octyl pyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate or acetonitrile;
Further, the stabilizer is selected from one or a mixture of more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate;
Further, the penetrating agent is selected from one or a mixture of more of penetrating agent JFC, penetrating agent T, azone or organic silicon;
further, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;
Further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;
all of the above are commercially available;
Further, the acaricidal composition can be prepared into any one of agriculturally acceptable preparation forms, wherein the preparation forms are any one of powder, granule, ball, tablet, strip, wettable powder, oil dispersion powder, emulsion powder, water dispersible granule, emulsion, water dispersible tablet, soluble powder, soluble tablet, soluble granule, soluble solution, oil, spreading oil, emulsifiable concentrate, emulsion, dispersible liquid, paste, aqueous emulsion, oil emulsion, microemulsion, lipid suspending agent, microcapsule suspending agent, oil suspending agent, dispersible oil suspending agent, suspoemulsion, microcapsule suspension-suspending agent, microcapsule suspension-aqueous emulsion or microcapsule suspension-suspending emulsion;
furthermore, the preparation formulation is any one of suspending agent, missible oil, wettable powder or water dispersible granule;
the invention also discloses the application of the acaricidal composition for controlling plant pest mites.
Further, the spider mites are tetranychidae mites, and the tetranychidae mites are panonychus citri, panonychus ulmi, cotton red mites, tetranychidae, and tetranychidae;
further, the tetranychidae mites are panonychus citri;
further, the plant is an economic crop and/or a food crop.
The invention has the beneficial effects that:
1) The acaricidal composition disclosed by the invention is reasonable in components, has an excellent control effect on multiple tetranychus mites such as panonychus citri, has a long lasting period, and can effectively control the harm of the mites;
2) The acaricidal composition is safe to target crops and almost has no phytotoxicity to the target crops.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Preparation example:
Preparation example 1:20% pyfluumbide azocyclotin suspension (1:1)
The formula comprises the following components: the weight percentages of the components are 10% pyfluumbide, 10% azocyclotin, 2% ammonium dodecyl sulfate, 1% naphthalene sulfonate formaldehyde condensate, 4% alkylphenol ethoxylate phosphate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% glycol, 0.1% sodium benzoate, 0.5% silicone oil and deionized water.
The preparation method comprises the following steps: according to the formula proportion, the active ingredients, the surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, high-speed shearing and wet sanding are carried out, and finally, the suspending agent product is obtained through homogenizing and filtering.
Preparation example 2:21% pyfluumbide azocyclotin suspension (1:6)
The formula comprises the following components: the weight percentages of the components are 3% of pyfluumbide, 18% of azocyclotin, 2% of isomeric tridecanol polyoxyethylene ether, 3% of alkylphenol polyoxyethylene ether phosphate, 2% of polycarboxylic acid sodium salt, 0.25% of xanthan gum, 1% of magnesium aluminum silicate, 4% of glycerol, 0.1% of sodium benzoate, 0.5% of silicone oil and deionized water.
The preparation method comprises the following steps: the same as in preparation example 1.
Preparation example 3:25% pyrfluumbide, azocyclotin emulsifiable concentrate (4:1)
The formula comprises the following components: the weight percentages of the components are 20% of pyfluumbide, 5% of azocyclotin, 12% of DMF, 12% of alkylphenol ethoxylates, 1% of calcium dodecyl benzene sulfonate, 15% of propylene carbonate and the balance of xylene.
The preparation method comprises the following steps: according to the formula proportion, adding the active ingredients, the solvent and the cosolvent into a blending kettle, stirring to dissolve the active ingredients, adding the emulsifier, supplementing the rest with the residual solvent, stirring uniformly in the stirring kettle, and filtering to obtain the required emulsifiable concentrate.
Preparation example 4:24% pyrfluumbide, azocyclotin emulsifiable concentrate (1:3)
The formula comprises the following components: the weight percentages are 6% pyfluumbide, 18% azocyclotin, 15% EO/PO block copolymer, 15% acetophenone, 10% N-methyl pyrrolidone, 12% castor oil polyoxyethylene ether, 2% calcium dodecyl benzene sulfonate and trimethylbenzene to make up the balance.
The preparation method comprises the following steps: the same as in preparation example 3.
Preparation example 5:30% pyfluumbide, azocyclotin water dispersible granule (1:5)
The formula comprises the following components: according to the weight percentage, 5% of pyfluumbide, 25% of azocyclotin, 3% of sodium dodecyl sulfate, 10% of sodium polycarboxylate, 8% of naphthalene sulfonate formaldehyde condensate, 5% of white carbon black and the balance of kaolin.
The preparation method comprises the following steps: according to the formula proportion, adding the active ingredients into a carrier, adding a surfactant and other functional additives into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to obtain the water dispersible granule product.
Preparation example 6:32% pyfluumbide, azocyclotin water dispersible granule (1:1)
The formula comprises the following components: the composition comprises, by weight, 16% pyfluumbide, 16% azocyclotin, 11% naphthalene sulfonate formaldehyde condensate, 3% sodium dodecyl sulfate, 6% sodium lignin sulfonate, 6% white carbon black, 32% starch, and kaolin balance.
The preparation method comprises the following steps: the same as in preparation example 5.
Preparation example 7:30% pyfluumbide, azocyclotin wettable powder (3:1)
The formula comprises the following components: 22.5% of pyfluumbide, 7.5% of azocyclotin, 3% of sodium lignin sulfonate, 5% of naphthalene sulfonate formaldehyde condensate, 3% of nekal BX and the balance of kaolin are added according to the weight percentage.
The preparation method comprises the following steps: mixing the effective components, the dispersing agent, the wetting agent and the filler according to the formula proportion, uniformly stirring in a stirring kettle, and carrying out multiple crushing and uniform mixing by a jet mill to obtain the wettable powder of the composition.
Preparation example 8:33% pyfluumbide, azocyclotin wettable powder (1:2)
The formula comprises the following components: 11% of pyfluumbide, 22% of azocyclotin, 7% of naphthalene sulfonate formaldehyde condensate, 2% of sodium dodecyl benzene sulfonate, 2% of nekal BX and the balance of kaolin.
The preparation method comprises the following steps: the same as in preparation example 7.
Indoor bioassay test
Example 1: pyflubumide and azocyclotin compound indoor activity test for panonychus citri
The test is based on: test reference NY/T1154.13-2008 "pesticide section 13, guidelines for indoor bioassay tests for pesticides: leaf disk spray method.
Test target: the citrus panonychus (Panonychus cirtri Wcgregov) is a female adult mite with consistent physiological state.
Test agent: 95% azocyclotin stock and 92% pyfluumbide stock are all provided by the group development center.
Preparing a test material: selecting citrus leaves with consistent growth, making leaf discs by using a puncher, placing a piece of wet sponge in a culture dish, placing filter paper on the wet sponge, placing leaf discs on the filter paper, 2 leaf discs in each dish, and connecting target pest mites fed indoors to the leaf discs, wherein 15 heads of each leaf disc are arranged.
And (3) preparation of a medicament: the above raw materials are dissolved by proper solvents, 5 groups of proportions are designed according to the mixing purpose and the activity of the medicament, and 5 series of mass concentration gradients are prepared for each single dose and each group of proportions.
The test method comprises the following steps: the Potter spray tower pressure was adjusted to a steady state of 1.47 x10 5 Pa, and the cleaned spray head was rinsed 2 times with acetone and then 2 times with distilled water.
The culture dish is placed on a Potter spray tower chassis for spraying, the spraying amount is 1mL, the liquid medicine is taken out after settling for 1min, each treatment is repeated for 4 times, and the treatment without the medicament is set as a blank control.
Feeding and observing: and (5) putting the treated test insects under proper conditions for feeding and observing. After 48 hours, the death of the mites was checked and the total number of insects and the number of dead insects were recorded, respectively.
The calculation method comprises the following steps:
mortality of each treatment was calculated from the survey data. Calculated as follows:
Wherein:
P-mortality in percent (%);
K-represents the number of dead insects in units of heads;
N-represents the total number of insects treated in units of heads.
Wherein:
p 1 -correct mortality in percent (%);
P t -mortality in percent (%);
p 0 -control mortality in percent (%).
If the control mortality is less than 5%, correction is not needed; the control mortality is between 5% and 20%, and correction is carried out according to a correction mortality formula; control mortality was > 20% and the test was reworked.
The LC 50 value was obtained by analysis using DPS statistical analysis system, and the activity of the test agent on the biological test material was evaluated.
The co-toxicity coefficient (CTC value) of the blend is calculated as follows:
Wherein:
Ati—actual measured virulence index of the mixture;
S-LC 50 of standard acaricide in milligrams per liter (mg/L);
M-LC 50 of the mix in milligrams per liter (mg/L).
TTI＝TIA×PA+TIB×PB
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A A agent toxicity index;
the percentage content of the P A -A medicament in the mixture is expressed as percentage (%);
TI B -agent toxicity index;
The percentage of the P B -B medicament in the mixture is expressed as percentage (%).
Wherein:
ctc—co-toxicity coefficient;
Ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The compound co-toxicity coefficient CTC is more than or equal to 120 and shows synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
The results of the indoor test are shown in the following table:
Table 1 pyflubumide and azocyclotin combination indoor Activity test results on Panonychus citri
From the results of the indoor activity assay, it was shown (see table 1) that panonychus citri is more sensitive to pyflubumide. Pyflubumide and azocyclotin are reasonably compounded, so that the compound pesticide has a good control effect on panonychus citri. pyflubumide to azocyclotin in a mass ratio of 1:45-50:1, and the co-toxicity coefficient is greater than 80, and the combined effect is shown as synergy or addition. pyflubumide to azocyclotin in a mass ratio of 1:35-30:1, and the co-toxicity coefficient is greater than 120, and the combined effect is synergistic.
Test for controlling efficacy in field
Example 2: pyflubumide and azocyclotin compounding field pesticide effect control test for panonychus citri
The test is based on: the test refers to GB/T17980.11-2000 "pesticide field efficacy test criterion (one) acaricide for controlling full-claw mites".
Test target: panonychus citri (Panonychus cirtri Wcgregov).
Test site: the test is carried out in the Guangxi Zhuang autonomous region Gui Linshi lingchuan county, ling Tian Xiangtang village citrus orchard, the soil fertility of the test site is medium and basically uniform, the irrigation field is good, and the ground is free of weeds and covers.
Test crop: nanfeng orange.
And (3) test design: the experiment was run using a random arrangement, with each treatment repeated 4 times and each cell area was 2 citrus trees. The backpack electric sprayer is adopted to uniformly spray the whole tree, so that the leaf surfaces, leaf backs, branches and fruit surfaces are not sprayed in a leakage way or in a heavy spraying way, and the whole tree is suitable for wetting without dripping water. The administration time was 21 days 4/2020, and the administration was only 1 time during the test.
The investigation method comprises the following steps: the young shoots are marked in the east, west, south, north and middle five directions of the tree in each cell, and the total number of the movable mites on 50 leaves is investigated in each cell. The number of the movable mites on the front and back sides of the blade is directly observed by using a handheld magnifier, and the number of the mites is counted. Each of 3d, 7d, and 15d was investigated once after the application treatment.
The control effect calculating method comprises the following steps:
The test results are shown in the following table:
Table 2pyflubumide and azocyclotin compounding field test results on Panonychus citri
The field efficacy test result shows that the acaricidal composition has good control effect on the panonychus citri, the control effect on the panonychus citri is more than 88% after the composition is applied for 15 days, the control effect is obvious and the lasting period is long.
Safety test in the field
Example 3: pyflubumide and azocyclotin compounding citrus safety test
Test crop: bei bei 447 jin orange.
Test site: the Daqing North medium district is a Mars town satellite village citrus garden.
Test agent: 21% of pyfluide-azocyclotin suspending agent (1:6), 25% of pyfluide-azocyclotin emulsifiable concentrate (4:1), 33% of pyfluide-azocyclotin wettable powder (1:2), 20% of pyfluide-azocyclotin suspending agent (1:1), 20% of pyfluide suspending agent and 20% of azocyclotin suspending agent.
And (3) test design: the experiment was run using a random arrangement, with each treatment repeated 4 times and each cell area was 2 citrus trees.
Time of application and investigation time: the test is carried out in the spring tip stage of the citrus, and the pesticide is applied by adopting a industrial pesticide-16 type knapsack manual sprayer, and the pesticide damage condition of each treatment of the citrus tender tip is investigated in 4d and 15d after the pesticide is applied; leaf phytotoxicity and spring tip length were investigated 30 days after drug administration, and leaf damage was graded.
The investigation method comprises the following steps: during investigation, 2 citrus trees are investigated by each treatment, spring tips are marked in five directions of east, south, west, north and middle of each citrus tree, 1 leaf on the spring tips is investigated in each direction, 10 leaf numbers on the spring tips are investigated in total by each treatment, the leaf numbers on the spring tips are recorded and investigated, the leaf numbers at all levels are counted, and the leaf colors, the branch colors, the leaf thicknesses and the like of the various treatments are compared. When the length of the young shoots was investigated, the lengths of 1 shoot were measured in five directions of east, south, west, north and middle of 1 plant on each treatment with a vernier caliper.
The degree of victimization is graded as follows:
level 0: the blade is not damaged;
stage 1: the leaf damage spot area occupies less than 1/4 of the leaf area;
2 stages: the area of the damaged spot occupies 1/4 to 1/2 of the leaf area;
3 stages: the area of the damaged spot occupies 1/2 to 3/4 of the leaf area;
4 stages: the area of the damaged spots occupies more than 3/4 of the leaf area.
The leaf damage index is calculated according to the following formula:
The test results are shown in the following table:
Table 3 pyflubumide investigation of phytotoxicity of citrus with azocyclotin
TABLE 4 effect of 30 days after the compounded formulation of pyflubumide and azocyclotin on tender tip leaf and tender tip length of citrus
Treatment of Index of damage Length of tender tip/mm
21% Pyfluumbide azocyclotin suspension (1:6) 1.69 166.75bc
25% Pyrfluumbide, azocyclotin emulsifiable concentrate (4:1) 1.81 175.25ab
33% Pyfluumbide, azocyclotin wettable powder (1:2) 1.92 182.08a
20% Pyfluumbide azocyclotin suspension (1:1) 1.85 184.50a
20% Azocyclotin suspending agent 36.41 123.80d
20% Pyfluumbide suspension 6.35 157.10c
As can be seen from tables 3 and 4, the mite-killing composition of the present invention treated group citrus showed no phytotoxicity after 4 days and 15 days after the drug, whereas 20% azocyclotin suspension produced phytotoxicity to citrus. 30d after the acaricide composition disclosed by the invention has almost no influence on citrus leaves, the damage index of the citrus leaves is not more than 2, the influence of pyfluumbide on the citrus leaves is small, the damage index is 6.35, the phytotoxicity of azocyclotin on the citrus leaves is the most serious, and the damage index is more than 36; as can be seen from comparison, the azocyclotin single dose has a certain inhibition effect on the growth of tender tips besides being harmful to the leaves.
In conclusion, through indoor activity tests and field tests, the acaricidal composition provided by the invention has excellent activity on plant mites, especially citrus panonychus citri, has remarkable control effect and long lasting period, is basically harmless to target crops, and is safe to crops.
While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto, and it is therefore intended that the invention as defined in the appended claims be interpreted as broadly as possible without departing from the spirit of the invention.

Claims (10)

1. The mite-killing composition containing azocyclotin is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is pyflubumide, the active ingredient B is azocyclotin, and the mass ratio of the active ingredient A to the active ingredient B is 1:35-30:1.
2. The acaricidal composition according to claim 1, wherein the mass ratio of the active ingredient a to the active ingredient B is 1:25 to 20:1.
3. The acaricidal composition according to claim 1, wherein the total weight of the acaricidal composition is 100 wt%, and the total weight of the active ingredient a and the active ingredient B is 2% -60% of the total weight of the acaricidal composition.
4. The acaricidal composition according to claim 1, wherein the total weight of the acaricidal composition is from 4% to 50% of the total weight of the acaricidal composition based on 100 wt% of the total weight of the active ingredient a and the active ingredient B.
5. The acaricidal composition according to claim 1, wherein the acaricidal composition comprises agriculturally acceptable auxiliary ingredients in addition to the active ingredient, the auxiliary ingredients being selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, anti-freeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.
6. The acaricidal composition according to claim 1, wherein the acaricidal composition can be prepared into any one of agriculturally acceptable formulation, and the formulation is any one of suspension, emulsifiable concentrate, wettable powder or water dispersible granule.
7. Use of a acaricidal composition according to any one of claims 1 to 6 for controlling plant pest mites.
8. The use according to claim 7, wherein the plant pest mites are spider mites and the spider mites are panonychus citri, panonychus ulmi, panonychus martensi, tetranychus cinnabarinus and/or tetranychus urticae.
9. The use according to claim 8, wherein the tetranychidae is panonychus citri.
10. The use according to claim 7, wherein the plant is an economic crop and/or a food crop.
CN202310422955.5A 2023-04-20 2023-04-20 Mite-killing composition containing azocyclotin and application thereof Active CN116369328B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007308470A (en) * 2005-08-12 2007-11-29 Nippon Nohyaku Co Ltd Substituted pyrazolecarboxylic acid anilide derivative or salt thereof, intermediate thereof, agent for agricultural and horticultural use, and use thereof
WO2008099820A1 (en) * 2007-02-14 2008-08-21 Nihon Nohyaku Co., Ltd. Pest control agent composition and use of the same
JP2011241183A (en) * 2010-05-19 2011-12-01 Nippon Nohyaku Co Ltd Amide derivative and agricultural/horticultural acaricidal agent comprising the derivative, and using method therefor
JP2014181205A (en) * 2013-03-19 2014-09-29 Nippon Nohyaku Co Ltd Agricultural and horticultural pest control agent composition and method for using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007308470A (en) * 2005-08-12 2007-11-29 Nippon Nohyaku Co Ltd Substituted pyrazolecarboxylic acid anilide derivative or salt thereof, intermediate thereof, agent for agricultural and horticultural use, and use thereof
WO2008099820A1 (en) * 2007-02-14 2008-08-21 Nihon Nohyaku Co., Ltd. Pest control agent composition and use of the same
JP2011241183A (en) * 2010-05-19 2011-12-01 Nippon Nohyaku Co Ltd Amide derivative and agricultural/horticultural acaricidal agent comprising the derivative, and using method therefor
JP2014181205A (en) * 2013-03-19 2014-09-29 Nippon Nohyaku Co Ltd Agricultural and horticultural pest control agent composition and method for using the same

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