CN116350857A - Anti-infection epidural catheter and manufacturing method - Google Patents
Anti-infection epidural catheter and manufacturing method Download PDFInfo
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- CN116350857A CN116350857A CN202310334712.6A CN202310334712A CN116350857A CN 116350857 A CN116350857 A CN 116350857A CN 202310334712 A CN202310334712 A CN 202310334712A CN 116350857 A CN116350857 A CN 116350857A
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- polyvinyl alcohol
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
- A61L29/085—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
- A61L29/10—Inorganic materials
- A61L29/106—Inorganic materials other than carbon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
- A61L29/16—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/62—Encapsulated active agents, e.g. emulsified droplets
- A61L2300/624—Nanocapsules
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses an anti-infection epidural catheter and a manufacturing method thereof, wherein an antibacterial surface coating is sprayed on the outer layer of the outer catheter; the antibacterial surface coating comprises the following components in parts by weight: 50-100 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 5-10 parts of film forming agent, 2-5 parts of dispersing agent and the balance of water; the invention belongs to the technical field of outer catheters; the invention adopts the gold nanoparticle composite modified polyvinyl alcohol polymer, wherein the gold nanoparticles have higher chemical stability and biocompatibility, and the frequency of inducing bacterial drug resistance is lower; the hydrophilic capacity of the polyvinyl alcohol is optimized through modification, and the polar hydrophilic group is introduced, so that the polyvinyl alcohol is easy to penetrate cell membranes, and the broad-spectrum antibacterial capacity is effectively enlarged; the gold nanoparticles and the modified polyvinyl alcohol polymer are compounded, and the gold nanoparticles and the modified polyvinyl alcohol polymer are taken as main bodies to construct the antibacterial surface coating, so that excellent antibacterial activity is shown.
Description
Technical Field
The invention belongs to the field of external catheters, and particularly relates to an anti-infection epidural catheter and a manufacturing method thereof.
Background
The epidural analgesia is an effective postoperative analgesia method, and particularly, the epidural analgesia pump is widely applied clinically in recent years, so that good news is brought to postoperative analgesia of patients; the external catheters mainly adopted in clinic at present comprise a steel wire reinforced type epidural catheter and a common polyvinyl chloride epidural catheter, and the harm to human bodies is larger and larger along with longer time of the catheter being placed in the human bodies; bacteria adhere to the surface of the epidural catheter during infection; more seriously, bacteria attached to the surface can generate extracellular polymeric substances to form a biological film composed of extracellular polymers such as proteins, and the biological film is difficult to eliminate, so that the bacteria can be protected from being affected by traditional antibacterial agents, infection is induced, and patients with epidural analgesia are in high risk of infection related to an external catheter; therefore, the anti-infection epidural catheter is provided, and has important significance for protecting the health of patients.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an anti-infection epidural catheter and a manufacturing method thereof, which effectively solve the problem that the epidural catheter is easy to be infected by bacteria; the invention adopts the gold nanoparticle composite modified polyvinyl alcohol polymer, wherein the gold nanoparticles have higher chemical stability and biocompatibility, and the frequency of inducing bacterial drug resistance is lower; the hydrophilic capacity of the polyvinyl alcohol is optimized through modification, and a polar hydrophilic group (amino group) is introduced, so that the polyvinyl alcohol is easy to permeate cell membranes, and the broad-spectrum antibacterial capacity is effectively enlarged; the gold nanoparticles and the modified polyvinyl alcohol polymer are compounded, the gold nanoparticles and the modified polyvinyl alcohol polymer are taken as main bodies to construct the antibacterial surface coating, and the antibacterial surface coating sprayed on the outer layer of the outer catheter can show excellent antibacterial activity.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the invention provides an anti-infection epidural catheter, wherein an antibacterial surface coating is sprayed on the outer layer of the outer catheter; the antibacterial surface coating comprises the following components in parts by weight: 50-100 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 5-10 parts of film forming agent, 2-5 parts of dispersing agent and the balance of water.
Further, the preparation process of the gold nanoparticle composite modified polyvinyl alcohol polymer comprises the following steps:
(1) Putting 1g of polyvinyl alcohol into a round-bottom flask, adding 20mL of N, N-dimethylformamide, stirring for 30 minutes at the temperature of 80 ℃, adding nitric acid to adjust the pH, adding ethanolamine, carrying out oil bath reaction for 12 hours at the temperature of 100-150 ℃, ending the reaction, filtering, washing 3 times with tetrahydrofuran, carrying out suction filtration, and drying in a vacuum oven at the temperature of 60 ℃ to prepare a modified polyvinyl alcohol polymer;
(2) Dispersing gold nanoparticles in deionized water to prepare a system A, adding modified polyvinyl alcohol into the system A, adding 1-ethyl- (3-dimethylaminopropyl) carbodiimide, stirring for 4 hours at room temperature, washing with water for 2 times after the reaction is finished, and centrifuging to prepare the gold nanoparticle composite modified polyvinyl alcohol polymer.
Preferably, the pH is 4.0-5.0.
Further, the preparation method of the gold nanoparticles comprises the following steps:
(1) Sequentially adding a tetrachloroauric acid solution and deionized water into dodecyl dimethyl benzyl ammonium chloride, and stirring for 30 minutes to prepare a solution A;
(2) Adding 10mg of carbon black into the solution A, adding 50mL of deionized water, and stirring at room temperature for reaction for 2 hours;
(3) And after the reaction is finished, filtering, washing with deionized water for 3 times, and drying in a vacuum oven at 60 ℃ for 6 hours to prepare the gold nanoparticles.
Preferably, the concentration of the tetrachloroauric acid solution is 15mmol/L.
Preferably, the centrifugation conditions are: the rotation speed was 11000 rpm and the time was 15 minutes.
Further, the film forming agent comprises one or more of styrene resin, isooctyl acrylate, urea-formaldehyde resin and ethylene glycol butyl ether.
Further, the dispersant comprises one or more combinations of one or more of sodium tripolyphosphate, sodium dodecyl sulfate, guar gum, and methylpentanol.
The invention also provides a preparation method of the antibacterial surface coating, which specifically comprises the following steps: and mixing the gold nanoparticle composite modified polyvinyl alcohol polymer, the dispersing agent and water, stirring for 30 minutes at room temperature, adding the film forming agent, heating to 40 ℃, and stirring for 1 hour to prepare the surface coating.
Further, the outer catheter adopts a low-flow medium-pressure spraying technology to spray the antibacterial surface coating on the outer layer of the outer catheter.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the invention, the gold nanoparticle composite modified polyvinyl alcohol polymer is adopted, the gold nanoparticle composite modified polyvinyl alcohol polymer is taken as a main body to construct the antibacterial surface coating, and the antibacterial surface coating sprayed on the outer layer of the outer catheter can show excellent antibacterial activity;
(2) The gold nanoparticles used in the invention have higher chemical stability and biocompatibility, and the frequency of inducing bacterial drug resistance is lower;
(3) According to the invention, the polyvinyl alcohol is adopted, the hydrophilic capacity is optimized through modification, and the polar hydrophilic group (amino) is introduced to prepare the modified polyvinyl alcohol polymer, so that the modified polyvinyl alcohol polymer is easy to penetrate cell membranes, and the broad-spectrum antibacterial capacity is effectively enlarged;
(4) The invention adopts the anti-infection epidural catheter, which can play a role in bacteriostasis and sterilization, thereby playing a role in preventing the possibility of infection; meanwhile, the preparation method is simple, the use effect is good, and the anti-infection effect can be well achieved.
Drawings
Fig. 1 is a graph of bacteriostatic properties of example 1, example 2, example 3, example 4 and comparative example 1;
FIG. 2 is a graph of the bacteriostatic properties of example 2 over time;
fig. 3 (a) and 3 (b) are scanning electron microscope images of staphylococcus aureus of example 2 and comparative example 1.
Detailed Description
The present invention will be described in further detail with reference to the following preferred examples, but the present invention is not limited to the following examples.
Unless otherwise specified, the chemical reagents involved in the present invention are all commercially available.
Polyvinyl alcohol (98%), 1-ethyl- (3-dimethylaminopropyl) carbodiimide (98%) and tetrachloroauric acid (97%) used in the present invention were purchased from a music research reagent; n, N-dimethylformamide (analytically pure) was purchased from aladine; tetrahydrofuran (analytically pure), ethanolamine (analytically pure) and nitric acid (90%) were purchased from merck pharmaceutical biotechnology company; dodecyl dimethyl benzyl ammonium chloride (98%) was purchased from Shanghai Jizhui Biochemical technologies Co.
Example 1
The invention provides an anti-infection epidural catheter
The outer layer of the outer catheter is sprayed with an antibacterial surface coating; the antibacterial surface coating comprises the following components in parts by weight: 50 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 5 parts of film forming agent, 2 parts of dispersing agent and the balance of water.
The preparation process of the gold nanoparticle composite modified polyvinyl alcohol polymer comprises the following steps:
(1) Putting 1g of polyvinyl alcohol into a round-bottom flask, adding 20mL of N, N-dimethylformamide, stirring for 30 minutes at the temperature of 80 ℃, adding nitric acid to adjust the pH to 4.0, adding 1g of ethanolamine, carrying out oil bath reaction for 12 hours at the temperature of 100 ℃, ending the reaction, filtering, washing 3 times with tetrahydrofuran, carrying out suction filtration, and drying in a vacuum oven at the temperature of 60 ℃ to obtain a modified polyvinyl alcohol polymer;
(2) Dispersing 0.5g of gold nanoparticles in 20mL of deionized water to prepare a system A, adding 0.5g of modified polyvinyl alcohol into the system A, adding 0.1g of 1-ethyl- (3-dimethylaminopropyl) carbodiimide, stirring at room temperature for 4 hours, filtering after the reaction is finished, and washing with water for 2 times to prepare the gold nanoparticle composite modified polyvinyl alcohol polymer.
The preparation method of the gold nanoparticles comprises the following steps:
(1) Sequentially adding 10mL of tetrachloroauric acid solution with the concentration of 15mmol/L and 50mL of deionized water into 0.2g of dodecyl dimethyl benzyl ammonium chloride, and stirring for 30 minutes to prepare a solution A;
(2) Adding 10mg of carbon black into the solution A, adding 50mL of deionized water, and stirring at room temperature for reaction for 2 hours;
(3) After the reaction, 11000 rpm, centrifuging for 15 minutes, washing with deionized water for 3 times, and drying in a vacuum oven at 60 ℃ for 6 hours to prepare gold nanoparticles.
Wherein the film forming agent is styrene resin; the dispersing agent is sodium tripolyphosphate.
The invention also provides a preparation method of the antibacterial surface coating, which specifically comprises the following steps: 50 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 2 parts of dispersing agent and water are mixed, stirred at room temperature for 30 minutes, 5 parts of film forming agent is added, the temperature is raised to 40 ℃, and the mixture is stirred for 1 hour, so that the surface coating is prepared.
The outer conduit adopts a low-flow medium-pressure spraying technology to spray the antibacterial surface coating on the outer layer of the outer conduit.
Example 2
The invention provides an anti-infection epidural catheter
The outer layer of the outer catheter is sprayed with an antibacterial surface coating; the antibacterial surface coating comprises the following components in parts by weight: 65 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 7 parts of film forming agent, 3 parts of dispersing agent and the balance of water.
The preparation process of the gold nanoparticle composite modified polyvinyl alcohol polymer comprises the following steps:
(1) Putting 1g of polyvinyl alcohol into a round-bottom flask, adding 20mL of N, N-dimethylformamide, stirring for 30 minutes at the temperature of 80 ℃, adding nitric acid to adjust the pH to 4.3, adding 1g of ethanolamine, carrying out oil bath reaction for 12 hours at the temperature of 120 ℃, ending the reaction, filtering, washing 3 times with tetrahydrofuran, carrying out suction filtration, and drying in a vacuum oven at the temperature of 60 ℃ to obtain a modified polyvinyl alcohol polymer;
(2) Dispersing 0.6g of gold nanoparticles in 20mL of deionized water to prepare a system A, adding 0.6g of modified polyvinyl alcohol into the system A, adding 0.1g of 1-ethyl- (3-dimethylaminopropyl) carbodiimide, stirring at room temperature for 4 hours, filtering after the reaction is finished, and washing with water for 2 times to prepare the gold nanoparticle composite modified polyvinyl alcohol polymer.
The preparation method of the gold nanoparticles comprises the following steps:
(1) 12mL of tetrachloroauric acid solution with the concentration of 15mmol/L and 50mL of deionized water are sequentially added into 0.2g of dodecyl dimethyl benzyl ammonium chloride, and the mixture is stirred for 30 minutes to prepare a solution A;
(2) Adding 10mg of carbon black into the solution A, adding 50mL of deionized water, and stirring at room temperature for reaction for 2 hours;
(3) After the reaction, 11000 rpm, centrifuging for 15 minutes, washing with deionized water for 3 times, and drying in a vacuum oven at 60 ℃ for 6 hours to prepare gold nanoparticles.
Wherein the film forming agent is isooctyl acrylate; the dispersing agent is sodium dodecyl sulfate.
The invention also provides a preparation method of the antibacterial surface coating, which specifically comprises the following steps: and mixing 65 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 3 parts of dispersing agent and water, stirring at room temperature for 30 minutes, adding 7 parts of film forming agent, heating to 40 ℃, and stirring for 1 hour to prepare the surface coating.
The outer conduit adopts a low-flow medium-pressure spraying technology to spray the antibacterial surface coating on the outer layer of the outer conduit.
Example 3
The invention provides an anti-infection epidural catheter
The outer layer of the outer catheter is sprayed with an antibacterial surface coating; the antibacterial surface coating comprises the following components in parts by weight: 80 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 8 parts of film forming agent, 4 parts of dispersing agent and the balance of water.
The preparation process of the gold nanoparticle composite modified polyvinyl alcohol polymer comprises the following steps:
(1) Putting 1g of polyvinyl alcohol into a round-bottom flask, adding 20mL of N, N-dimethylformamide, stirring for 30 minutes at the temperature of 80 ℃, adding nitric acid to adjust the pH to 4.5, adding 1g of ethanolamine, carrying out oil bath reaction for 12 hours at the temperature of 130 ℃, ending the reaction, filtering, washing 3 times with tetrahydrofuran, carrying out suction filtration, and drying in a vacuum oven at the temperature of 60 ℃ to obtain a modified polyvinyl alcohol polymer;
(2) Dispersing 0.7g of gold nanoparticles in 20mL of deionized water to prepare a system A, adding 0.7g of modified polyvinyl alcohol into the system A, adding 0.1g of 1-ethyl- (3-dimethylaminopropyl) carbodiimide, stirring at room temperature for 4 hours, filtering after the reaction is finished, and washing with water for 2 times to prepare the gold nanoparticle composite modified polyvinyl alcohol polymer.
The preparation method of the gold nanoparticles comprises the following steps:
(1) 13mL of tetrachloroauric acid solution with the concentration of 15mmol/L and 50mL of deionized water are sequentially added into 0.2g of dodecyl dimethyl benzyl ammonium chloride, and the mixture is stirred for 30 minutes to prepare a solution A;
(2) Adding 10mg of carbon black into the solution A, adding 50mL of deionized water, and stirring at room temperature for reaction for 2 hours;
(3) After the reaction, 11000 rpm, centrifuging for 15 minutes, washing with deionized water for 3 times, and drying in a vacuum oven at 60 ℃ for 6 hours to prepare gold nanoparticles.
Wherein the film forming agent is urea formaldehyde resin; the dispersing agent is Gum.
The invention also provides a preparation method of the antibacterial surface coating, which specifically comprises the following steps: mixing 80 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 4 parts of dispersing agent and water, stirring for 30 minutes at room temperature, adding 8 parts of film forming agent, heating to 40 ℃, and stirring for 1 hour to prepare the surface coating.
The outer conduit adopts a low-flow medium-pressure spraying technology to spray the antibacterial surface coating on the outer layer of the outer conduit.
Example 4
The invention provides an anti-infection epidural catheter
The outer layer of the outer catheter is sprayed with an antibacterial surface coating; the antibacterial surface coating comprises the following components in parts by weight: 100 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 10 parts of film forming agent, 5 parts of dispersing agent and the balance of water.
The preparation process of the gold nanoparticle composite modified polyvinyl alcohol polymer comprises the following steps:
(1) Putting 1g of polyvinyl alcohol into a round-bottom flask, adding 20mL of N, N-dimethylformamide, stirring for 30 minutes at the temperature of 80 ℃, adding nitric acid to adjust the pH to 5.0, adding 1g of ethanolamine, carrying out oil bath reaction for 12 hours at the temperature of 150 ℃, ending the reaction, filtering, washing 3 times with tetrahydrofuran, carrying out suction filtration, and drying in a vacuum oven at the temperature of 60 ℃ to obtain a modified polyvinyl alcohol polymer;
(2) Dispersing 0.8g of gold nanoparticles in 20mL of deionized water to prepare a system A, adding 0.8g of modified polyvinyl alcohol into the system A, adding 0.1g of 1-ethyl- (3-dimethylaminopropyl) carbodiimide, stirring at room temperature for 4 hours, filtering after the reaction is finished, and washing with water for 2 times to prepare the gold nanoparticle composite modified polyvinyl alcohol polymer.
The preparation method of the gold nanoparticles comprises the following steps:
(1) 15mL of tetrachloroauric acid solution with the concentration of 15mmol/L and 50mL of deionized water are sequentially added into 0.2g of dodecyl dimethyl benzyl ammonium chloride, and the mixture is stirred for 30 minutes to prepare a solution A;
(2) Adding 10mg of carbon black into the solution A, adding 50mL of deionized water, and stirring at room temperature for reaction for 2 hours;
(3) After the reaction, 11000 rpm, centrifuging for 15 minutes, washing with deionized water for 3 times, and drying in a vacuum oven at 60 ℃ for 6 hours to prepare gold nanoparticles.
Wherein the film forming agent is ethylene glycol butyl ether; the dispersing agent is methyl amyl alcohol.
The invention also provides a preparation method of the antibacterial surface coating, which specifically comprises the following steps: mixing 100 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 5 parts of dispersing agent and water, stirring for 30 minutes at room temperature, adding 10 parts of film forming agent, heating to 40 ℃, and stirring for 1 hour to prepare the surface coating.
The outer conduit adopts a low-flow medium-pressure spraying technology to spray the antibacterial surface coating on the outer layer of the outer conduit.
Comparative example 1
This comparative example provides a general epidural catheter which differs from example 1 in that the outer layer of the outer catheter does not contain an antimicrobial surface coating and the remainder is the same as example 1.
Performance test (1)
To test the antibacterial effect of the anti-infective epidural catheter prepared by the embodiment of the invention, the killing effect of the anti-infective epidural catheter prepared by the embodiment 1, the embodiment 2, the embodiment 3, the embodiment 4 and the comparative embodiment 1 on staphylococcus aureus is experimentally measured; inoculating a small amount of standard strain (ATCC 25923) of staphylococcus aureus with seed retention into 10mL of pancreatic protein dolphin soybean broth nutrient solution, placing in an incubator for overnight culture, inoculating a trace bacterial liquid four-zone streak separation method into a blood plate culture medium, culturing overnight again in the incubator to obtain viable bacteria, diluting staphylococcus aureus density with 0.9% saline water, obtaining 2mL of 1×10 8 CFU/mL of the bacterial suspension, 20uL of the bacterial suspension was smeared outside the outer catheter, and the results were observed after culturing in a bacterial incubator at 37℃for 6 hours.
Bacterial culture and count: the outer catheters were placed and immersed in a sealed 50mL flat bottom graduated tube containing 20mL of phosphate buffer solution, and were subjected to shaking and washing for 15 minutes using an ultrasonic shaker, and after 500-fold dilution of the washing liquid with PBS solution, 100uL of the dilution was spread and inoculated into a common agar plate, placed in a bacterial incubator at 37 ℃ for 24 hours, and subjected to plate colony counting by using Colony Forming Units (CFU).
Results analysis, through the figures 1, 2 and 3, the bacteria culture results of the anti-infection epidural catheter prepared by the example 1, the example 2, the example 3 and the example 4 are obviously less than that of the common epidural catheter prepared by the comparative example 1 after the same bacteria inoculation time, which shows that the anti-infection epidural catheter provided by the invention has definite in vitro antibacterial performance; in comparison of the results of the bacterial culture after the different inoculation times in experimental example 2, the bacterial culture results with the inoculation time of 6 hours are obviously less than those with the inoculation time of 2 hours, which shows that the anti-infection epidural catheter provided by the invention has continuous antibacterial performance in the early stage of bacterial infection in vitro.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The invention and its embodiments have been described above with no limitation, and the actual construction is not limited to the embodiments of the invention as shown in the drawings. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present invention.
Claims (10)
1. An anti-infective epidural catheter, characterized in that the outer layer of the outer catheter is sprayed with an antibacterial surface coating; the antibacterial surface coating comprises the following components in parts by weight: 50-100 parts of gold nanoparticle composite modified polyvinyl alcohol polymer, 5-10 parts of film forming agent, 2-5 parts of dispersing agent and the balance of water.
2. The anti-infective epidural catheter of claim 1, wherein the preparation process of the gold nanoparticle composite modified polyvinyl alcohol polymer comprises the following steps:
(1) Putting 1g of polyvinyl alcohol into a round-bottom flask, adding 20mL of N, N-dimethylformamide, stirring for 30 minutes at the temperature of 80 ℃, adding nitric acid to adjust the pH, adding ethanolamine, carrying out oil bath reaction for 12 hours at the temperature of 100-150 ℃, ending the reaction, filtering, washing 3 times with tetrahydrofuran, carrying out suction filtration, and drying in a vacuum oven at the temperature of 60 ℃ to prepare a modified polyvinyl alcohol polymer;
(2) Dispersing gold nanoparticles in deionized water to prepare a system A, adding modified polyvinyl alcohol into the system A, adding 1-ethyl- (3-dimethylaminopropyl) carbodiimide, stirring for 4 hours at room temperature, washing with water for 2 times after the reaction is finished, and centrifuging to prepare the gold nanoparticle composite modified polyvinyl alcohol polymer.
3. An anti-infective epidural catheter according to claim 2, wherein the pH is 4.0-5.0.
4. An anti-infective epidural catheter according to claim 3, wherein the preparation method of gold nanoparticles comprises the following steps:
(1) Sequentially adding a tetrachloroauric acid solution and deionized water into dodecyl dimethyl benzyl ammonium chloride, and stirring for 30 minutes to prepare a solution A;
(2) Adding 10mg of carbon black into the solution A, adding 50mL of deionized water, and stirring at room temperature for reaction for 2 hours;
(3) And after the reaction is finished, centrifuging, washing with deionized water for 3 times, and drying in a vacuum oven at 60 ℃ for 6 hours to prepare the gold nanoparticles.
5. An anti-infective epidural catheter according to claim 4, wherein the concentration of tetrachloroauric acid solution is 15mmol/L.
6. An anti-infective epidural catheter of claim 5, wherein the centrifugation conditions: the rotation speed was 11000 rpm and the time was 15 minutes.
7. An anti-infective epidural catheter of claim 6, wherein: the film forming agent comprises one or more of styrene resin, isooctyl acrylate, urea-formaldehyde resin and ethylene glycol butyl ether.
8. An anti-infective epidural catheter of claim 7, wherein: the dispersing agent comprises one or more of sodium tripolyphosphate, sodium dodecyl sulfate, guar gum and methyl amyl alcohol.
9. An anti-infective epidural catheter of claim 8, wherein: the preparation method of the antibacterial surface coating comprises the following steps: and mixing the gold nanoparticle composite modified polyvinyl alcohol polymer, the dispersing agent and water, stirring for 30 minutes at room temperature, adding the film forming agent, heating to 40 ℃, and stirring for 1 hour to prepare the surface coating.
10. An anti-infective epidural catheter according to claim 9, wherein the outer catheter is sprayed with the antimicrobial surface coating using a low flow medium pressure spray technique.
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| CN1899629A (en) * | 2006-06-27 | 2007-01-24 | 杨玉民 | Anti-infection hard film external catheter and producing method |
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