CN116333888B - Fungus of family Botrytis and application - Google Patents
Fungus of family Botrytis and application Download PDFInfo
- Publication number
- CN116333888B CN116333888B CN202210990293.7A CN202210990293A CN116333888B CN 116333888 B CN116333888 B CN 116333888B CN 202210990293 A CN202210990293 A CN 202210990293A CN 116333888 B CN116333888 B CN 116333888B
- Authority
- CN
- China
- Prior art keywords
- fusarium
- wind
- fungus
- proof
- soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000233866 Fungi Species 0.000 title claims abstract description 40
- 241001465180 Botrytis Species 0.000 title description 2
- 241000196324 Embryophyta Species 0.000 claims abstract description 50
- 239000002689 soil Substances 0.000 claims abstract description 50
- 239000000725 suspension Substances 0.000 claims abstract description 31
- 241000223221 Fusarium oxysporum Species 0.000 claims abstract description 23
- 241000879295 Fusarium equiseti Species 0.000 claims abstract description 21
- 230000012010 growth Effects 0.000 claims abstract description 20
- 244000053095 fungal pathogen Species 0.000 claims abstract description 17
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000223218 Fusarium Species 0.000 claims abstract description 14
- 230000003405 preventing effect Effects 0.000 claims abstract description 14
- 239000003814 drug Substances 0.000 claims abstract description 12
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 10
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 10
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 230000001737 promoting effect Effects 0.000 claims abstract description 7
- 230000001580 bacterial effect Effects 0.000 claims description 29
- 241001180876 Saposhnikovia Species 0.000 claims description 17
- 241000229179 Ledebouriella Species 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 241000202758 Scirpus Species 0.000 claims 1
- 240000006365 Vitis vinifera Species 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 241001103785 Sirastachys castanedae Species 0.000 abstract description 25
- 230000000694 effects Effects 0.000 abstract description 23
- 244000005700 microbiome Species 0.000 abstract description 11
- 241000219095 Vitis Species 0.000 abstract description 9
- 208000031888 Mycoses Diseases 0.000 abstract description 8
- 230000002265 prevention Effects 0.000 abstract description 8
- 238000004321 preservation Methods 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 description 26
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 26
- 238000011282 treatment Methods 0.000 description 22
- 239000001965 potato dextrose agar Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 230000003042 antagnostic effect Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 241000223260 Trichoderma harzianum Species 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 9
- 230000000844 anti-bacterial effect Effects 0.000 description 9
- 239000000575 pesticide Substances 0.000 description 8
- 239000004563 wettable powder Substances 0.000 description 8
- 244000063299 Bacillus subtilis Species 0.000 description 7
- 239000005802 Mancozeb Substances 0.000 description 7
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 7
- 229960001225 rifampicin Drugs 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 6
- 239000006013 carbendazim Substances 0.000 description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000006806 disease prevention Effects 0.000 description 5
- 230000000877 morphologic effect Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 241001279361 Stachybotrys Species 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000008485 antagonism Effects 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000443 biocontrol Effects 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 241000235349 Ascomycota Species 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 241001103745 Sirastachys Species 0.000 description 2
- 241000347475 Stachybotryaceae Species 0.000 description 2
- 239000012681 biocontrol agent Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 235000016657 Bacillus subtilis BSn5 Nutrition 0.000 description 1
- 244000051262 Bacillus subtilis BSn5 Species 0.000 description 1
- 241001674808 Biastes Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 108020000949 Fungal DNA Proteins 0.000 description 1
- 108091023242 Internal transcribed spacer Proteins 0.000 description 1
- 241001184198 Orthosiphon Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 241001180873 Saposhnikovia divaricata Species 0.000 description 1
- 241001326533 Sordariomycetes Species 0.000 description 1
- 241001598044 Stachybotrys longispora Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 239000005855 Trichoderma harzianum strains T-22 and ITEM 908 Substances 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000035605 chemotaxis Effects 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a grape spike mould fungus and application thereof, belongs to the technical field of microorganisms, and solves the problem that no biological prevention and control of fungal diseases such as wind-proof fusarium wilt and root rot exist in the prior art. The invention discloses a grape spike mould fungus (Sirastachys castanedae) MR-43 with the preservation number of: GDMCC No.62081. The invention discloses an application of the strain or spore suspension thereof in preparing a medicament for preventing and treating pathogenic fungi of radix sileris, an application in preparing a medicament for preventing and treating fusarium wilt of radix sileris or/and root rot of radix sileris, and an application in preparing a preparation for promoting growth of radix sileris plants. The grape spike mould fungus MR-43 can stably propagate in the wind-proof cultivation soil, has good colonization capability, has a bacteriostasis rate of more than 57% on fusarium oxysporum and fusarium equisetum, can effectively control wind-proof fusarium wilt and wind-proof root rot, has an average control effect of 68.52%, and has an obvious promotion effect on the growth of wind-proof plants.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a grape spike mould fungus Sirastachys castanedae MR-43 and application thereof.
Background
Fusarium oxysporum (F.oxysporum) and Fusarium equisetum (F.equiseti) belong to Fusarium, are soil-borne pathogenic fungi with worldwide distribution and high hazard, have wide parasitic hosts and can cause diseases of hundreds of crops such as agriculture, facility gardening, medicinal plants and the like. The radix sileris Saposhnikovia divaricata (Turcz.) Schisck is one of important medicinal plant resources in China, is mainly distributed in northeast China, hebei China and other areas, is easily infected by fusarium oxysporum and overwintering spores of fusarium equiseti to induce radix sileris wilt and root rot, and has a annual incidence rate of more than 15-20 percent, so that the yield and quality of the radix sileris medicine source are seriously affected.
Can effectively prevent and control plant fungal diseases by using chemical approaches. In the prior art, chemical pesticides are taken as the dominant agents and still are the main prevention and control strategies for plant diseases at present, but the problems of environmental pollution, drug resistance enhancement and the like caused by the chemical pesticides are increasingly remarkable. The method for preventing and controlling plant diseases by utilizing the probiotics has the advantages of good effect, high ecological safety, obvious economic benefit and the like, accords with the green and safe plant protection concept, is an ideal alternative means for reducing the use of chemical pesticides and improving the sustainability of agricultural production at present, and is an important research direction in the field of plant protection at present.
The plant rhizosphere is an important area for carrying out substance communication between the plant root system and the soil, and root secretions released by plant bodies into the soil in the growth and development process have enrichment chemotaxis effect on soil microorganisms. Compared with the non-rhizosphere area of the plant, the relative abundance and diversity of microorganisms in rhizosphere soil are higher, and beneficial microorganisms which are active on the surface of plant root systems and rhizosphere soil obviously influence the growth and development of the plant and the quality formation by activating soil nutrients, regulating and controlling plant soil microecology and other mechanisms, so that the forward promotion effect on the growth of the plant body is finally realized. In addition, the growth of plant pathogenic microorganisms can be inhibited by rhizosphere beneficial microorganisms through space and nutrition competition, re-mailing action, antagonism, induction of plant disease resistance and the like. Therefore, research on prevention and control of plant fungal diseases using plant rhizosphere beneficial microorganisms is attracting attention of researchers. However, researches on biological prevention and control of fungal diseases such as wind blight, root rot and the like are reported at home and abroad.
Therefore, the development of the beneficial microorganism resources in the windbreak rhizosphere soil, the acquisition of the biocontrol bacteria source with better bacteriostasis on windbreak fusarium wilt and root rot, and the problem to be solved by the person skilled in the art are urgent.
Disclosure of Invention
The invention aims to provide a grape spike mould fungus (Sirastachys castanedae) MR-43 which is separated from wind-proof healthy rhizosphere soil, has remarkable antibacterial activity on fusarium oxysporum and fusarium equisetum, can effectively control wind-proof fusarium wilt and wind-proof root rot, has an obvious promotion effect on the growth of wind-proof plants, and solves the problem that no biological prevention and control on fungal diseases such as wind-proof fusarium wilt, root rot and the like exist in the prior art.
The second object of the present invention is to provide the use of the strain.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the invention discloses a strain of grape spike mould fungus (Sirastachys castanedae) MR-43, which is preserved in the microorganism preservation center of Guangdong province at the year 11 and the month 26 of 2021, and has the preservation number of: GDMCC No.62081, address: guangdong province.
The Stachybotrysaceae (Stachybotryoceae) fungus is one of the members of the saprophytic bacteria commonly present in the soil. In the research of researching the soil beneficial microorganism resource of the Jilin Changchun divaricate saposhnikovia root cultivation base, 1 strain of rhizosphere fungus MR-43 with strong antagonism to pathogenic bacteria of common fungal diseases such as Ledebouriella and Fusarium equisetum is obtained by screening, and the strain MR-43 is identified as Sirastachys castanedae based on culture characteristics, microscopic morphological characteristics and molecular biological means. The invention discovers that the fungus of the family Stachybotrys can be used as indigenous fungus to be planted in plant rhizosphere soil for the first time. Sirastachys castanedae is belonging to the order of the Hypocrea (Hypocreatles), stachybotrys (Stachybotryaceae), sirastachys group evolution branches in the phylum Ascomycota (Ascomycota), the phylum Pezizomycetina (Pezizomycetina), the class of Phycomycetes (Sordariomycetes).
The invention discloses application of a grape spike mould fungus (Sirastachys castanedae) MR-43 or spore suspension thereof in preparing a medicament for preventing and treating wind-proof pathogenic fungi.
In some embodiments of the invention, the wind-resistant pathogenic fungi include at least one of fusarium oxysporum Fusarium oxysporum, fusarium equiseti f.
In some embodiments of the invention, the medicament for controlling pathogenic fungi of Ledebouriella is applied to the soil of Ledebouriella cultivation.
The invention discovers that the bacterial colony edges of fusarium oxysporum and fusarium equiseti after the action of the strain MR-43 are thinned and tend to be clear, and aerial hyphae are dissolved and die. The S.castanedae MR-43 can destroy the cell wall or cell membrane structure of the mycelium of the pathogenic fungi by generating certain secondary metabolites to realize the action mechanism for inhibiting the growth of the pathogenic fungi, thereby suggesting that the S.castanedae extracellular secondary metabolites have the development potential of plant disease biocontrol agents.
The invention discloses an application of a grape spike mould fungus (Sirastachys castanedae) MR-43 or spore suspension thereof in preparing a medicament for preventing and treating wind-proof fusarium wilt or/and wind-proof root rot.
At present, the problem that the indoor antibacterial effect of a biocontrol bacterial source is obvious, but the outdoor potting or field control effect is low exists in the research of biocontrol of plant diseases. The invention uses MR-43 spore suspension, which proves that S.castanedae can effectively prevent and control wind-proof fusarium wilt and root rot for the first time, the disease prevention effect of the outdoor potted plant is still higher than the indoor antibacterial effect, and the disease management effect of the outdoor potted plant is equal to that of the conventional chemical pesticide.
The invention discloses application of a grape spike mould fungus (Sirastachys castanedae) MR-43 or spore suspension thereof in preparation of a preparation for promoting growth of a divaricate saposhnikovia root plant.
The invention discovers that the MR-43 of the Stachybotrys has a promoting effect on the growth of the divaricate saposhnikovia root, and the effect is better than that of B.subtilis and T.harzianum which are registered by pesticide registration.
In some embodiments of the invention, the drug for preventing and treating the windbreak wilt, the drug for preventing and treating the windbreak root rot and the preparation for promoting the growth of the windbreak plants are all applied to the windbreak cultivation soil.
In some embodiments of the invention, the spore suspension has a bacterial content of 1X 10 6 ～1×10 8 CFU/mL; preferably 1X 10 7 CFU/mL。
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, 1 strain of Stachybotrys castaneae (Stachybotryaceae) fungi S.Sirastachys castaneae MR-43,Sirastachys castanedae MR-43 is separated and screened from the windproof rhizosphere soil for the first time, and the stable propagation in the windproof cultivation soil can be realized, so that the stable propagation has good colonization capability.
The bacterial strain Sirastachys castanedae MR-43 has the bacteriostasis rate of more than 57% on fusarium oxysporum and fusarium equisetum, and has stable bacteriostasis effect after multiple times of verification; the spore suspension can effectively control the windbreak wilt and the windbreak root rot, the average control effect reaches 68.52 percent, and the disease prevention effect of the spore suspension is not obviously different from that of carbendazim and mancozeb (p is more than 0.05); in addition, MR-43 has obvious promotion effect on the growth of the divaricate saposhnikovia root plants. Therefore, the strain Sirastachys castanedae MR-43 has good development value and application potential in the aspect of fungal disease management such as windbreak blight, root rot and the like.
Drawings
FIG. 1 is a graph showing the effect of MR-43 of Sirastachys castanedae strain of the invention on growth of Fusarium oxysporum and Fusarium equisetum; wherein A: pure culture of fusarium oxysporum; b: fusarium oxysporum co-cultured with MR-43; c: pure culture of fusarium equiseti; d: fusarium equisetum was co-cultured with MR-43.
FIG. 2 is a graph showing colony morphology and microscopic morphology characteristics of the Sirastachys castanedae strain MR-43 of the present invention, wherein A: the front surface of the bacterial colony; b: the back of the bacterial colony; c: conidium; D. e: conidiophores; f: a spore-producing bottle body; reference scale: C. d, E =10 μm; f=5 μm.
FIG. 3 is a phylogenetic tree diagram for constructing MR-43 kindred relationship based on ITS sequences.
FIG. 4 is a graph showing the colonization of the strain MRRif-43 in soil.
FIG. 5 is a graph showing the control effect of the Sirastachys castanedae bacterium MR-43 of the present invention on wind root rot, wherein CK: treating with clear water; a: trichoderma harzianum; b: bacillus subtilis; c:70% mancozeb wettable powder.
FIG. 6 is a graph showing the effect of the Sirastachys castanedae bacterium MR-43 of the invention on preventing and controlling the windbreak wilt; wherein CK: treating with clear water; a: trichoderma harzianum; b: bacillus subtilis; c:50% carbendazim wettable powder.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1
The example discloses a method for separating and screening Sirastachys castanedae bacteria MR-43.
1. Test material
Collecting a soil sample: in the experimental field (43 DEG 48 '24' N, 125 DEG 24 '59' E, altitude 251 m) for wind-proof cultivation at the agricultural university of Jilin, changchun, jilin province 6 months 2020, a five-point sampling method is combined with a shaking-off method, collecting rhizosphere soil (soil 0-3 mm away from the surfaces of main roots and fibrous roots) of healthy divaricate saposhnikovia roots, uniformly mixing collected soil samples, placing the obtained mixture into a sterile self-sealing bag, and storing the obtained mixture at a temperature of 4 ℃ for later use, wherein the number of the collected soil samples is MR.
Test pathogenic fungi: fusarium oxysporum Fox201 and Fusarium equiseti Feq112 are pathogenic bacteria of Ledebouriella root rot, respectively, and are provided by Jilin agricultural university plant pathology laboratory.
Test medium: potato dextrose agar (Potato Dextrose Agar, PDA): peeling 200g of potato, 20.0g of glucose, 17.0g of agar and 1000mL of deionized water; potato dextrose water (Potato Dextrose Broth, PDB): peeled potato 200.0g, glucose 20.0g, deionized water 1000mL.
Test agent: mancozeb wettable powder (70%, mancozeb, WP), sichuan Runner technologies Co., ltd; carbendazim wettable powder (50%, carbendzim, WP), jiangsu Fengshan group, inc; trichoderma harzianum T-22 (50 hundred million/g of active bacteria), shandong Lv Long biotechnology Co., ltd; bacillus subtilis BSn5 (effective viable count 200 hundred million/g), shandong Orthosiphon Biotech Limited liability company; 97% rifampicin (Rif), shanghai microphone, biochemical technologies Co., ltd; DNAMaroer and PCR amplification kit, taKaRa, japan; PCR product purification kit and ITS synthetic primers, manufactured and bioengineered (Shanghai) Co., ltd.
2 test method
2.1 isolation, purification and preservation of rhizosphere fungi
The rhizosphere soil fungi are isolated under aseptic conditions by a dilution plate method. Taking a soil sample to be tested and sterile water according to the following ratio of 1: mixing the materials in a sterile conical flask according to a proportion of 9, sufficiently oscillating for 10min, and standing. Gradient dilution of the resulting soil suspension to 10 3 、10 4 、10 5 Doubling the liquid for standby. Respectively sucking 200 mu L of diluted solutions of different gradients, coating the diluted solutions in a PDA, culturing the diluted solutions in an inverted mode at the constant temperature of 25 ℃ for 5-7 d, and then picking a colony with fungus characteristics, purifying the colony on a PDA flat plate, and preserving the colony with the number of MR at-20 ℃ for later use.
2.2 screening for antagonistic fungi
The method of counter culture is adopted. Under aseptic conditions, preparing rhizosphere fungi, fusarium oxysporum and fusarium equiseti to be tested after activation culture into bacterial cakes with the diameter of 8mm by using a puncher, respectively inoculating 2 pathogenic fungi at the center of a PDA flat plate with the diameter of 90mm, inoculating rhizosphere fungi at a symmetrical point at the position 30mm away from the center of the flat plate, repeating the treatment for 3 times by taking single pathogenic fungi as a reference, culturing at the constant temperature of 21-25 ℃ for 7d, observing and recording the bacteriostasis condition, and calculating the bacteriostasis rate, wherein the calculation formula is as follows:
antibacterial ratio = (RC-RP)/RC 100%
Where RC is the radius of the comparison trend, RP is the radius of the treatment trend, and the units are all mm.
The applicant separates and purifies 104 strains of fungi in the wind-proof rhizosphere soil, wherein 7 strains of rhizosphere fungi have antagonism to fusarium oxysporum and fusarium equisetum, and the bacteriostasis rate is 9.91-61.85%. The bacterial inhibition rate of the strain MR-43 to fusarium oxysporum reaches more than 56%, and the bacterial inhibition activity of the strain MR-43 is obviously higher than that of the rest 6 antagonistic fungi, and the bacterial inhibition rate is 59.63% (p < 0.05); and the average antibacterial rate of the MR-43 strain to fusarium oxysporum and fusarium equisetum is 58.34 percent. MR-43 was shown to be effective in controlling the growth and proliferation of 2 pathogenic fungi (FIG. 1). They were therefore used as candidate strains for subsequent studies.
TABLE 1 inhibition of pathogenic fungi by strain MR-43
Example 2
The present example discloses an identification method of Sirastachys castanedae bacterium MR-43 of the present invention.
1. Morphological observation
Under aseptic conditions, preparing activated antagonistic fungus MR-43 into a bacterial cake with the diameter of 8mm, inoculating the bacterial cake to the center of a PDA culture medium plate, repeating for 3 times, placing the bacterial cake at the constant temperature of 25 ℃ for inverted dark culture, and observing the colony morphology, color and other characteristics of the antagonistic fungus MR-43 day by day; the culture is picked for tabletting, and morphological characteristics such as hypha, conidium and the like are observed in a microscopic way.
After the MR-43 colony is inversely cultured for 7 days at 25 ℃ on a PDA plate, the colony is flat and compact, secretion permeates into the PDA, the edge is irregular, and the colony is white (shown in A in figure 2); the colony had radial folds on its back and was orange-red (shown as B in fig. 2). Conidium ellipses 5-7×2-4 μm (shown as C in FIG. 2), conidium is straight or slightly curved (shown as D, E in FIG. 2), conidium bottle is monospore and rotifer, 3-10 conidium bottles per round, the bottom is tapered, the top is slightly enlarged, and the bottle is 9-14×2-4 μm (shown as F in FIG. 2). The results showed that the strain MR-43 had a morphology similar to that of the fungus of the Vitaceae family.
2. Molecular biological identification
The activated antagonistic fungus MR-43 was inoculated into PDB medium, cultured at 25℃under shaking at 150r/min for 5 days, mycelia were collected, fungal genomic DNA was extracted using TaKaRa MiniBEST Universal Genomic DNA Extraction Kit Ver.5.0 kit (Takara Bio, japan), and the DNA mass was detected by 1% agarose gel electrophoresis.
Amplification of antagonistic fungal DNA based on ITS-PCR, the primers were:
ITS1(5′-TCCGTAGGTGAACCTGCGG-3′)
ITS4(5′-TCCTCCGCTTATTGATATGC-3′)。
the reaction system: master Mix 12.5 μ L, ITS 11 μ L, ITS4 1 μ L, rDNA 2 μ L, ddH 2 O8.5. Mu.L, amplification procedure: 94 ℃ for 3min;94℃30s,55℃30s,72℃1min,30 cycles; extending at 72 ℃ for 5min, and preserving at 4 ℃. The PCR amplified products were sequenced by Shanghai workers.
Sequence homology comparison and cluster analysis: submitting the antagonistic fungus MR-43 sequencing result to NCBI nucleotide database and performing BIAST comparison analysis; clustal W multiplex alignment was performed using MEGA 5.2 software (https:// www.megasoftware.net/megamac. Php, arizona State University) and phylogenetic trees were constructed using the proximity method (Neighbor-Joining).
The result shows that the ITS gene sequence of the strain MR-43 is amplified by PCR and sequenced to obtain the base sequence with 540bp, and the GenBank accession number is OK287148.1. The sequence uploaded to NCBI is subjected to BLAST comparison, the homology with Sirastachys castanedae (KU 846661.1) reaches more than 99%, and the homology with S.pseudoolongispora (KU 846674.1), S.longispora (AF 081482.1) and S.parvisspora (KX 690114.1) is 97% -98%; based on the construction of phylogenetic tree, the results show that strain MR-43 shows a high degree of homology with S.castanedae (MW 793377.1) and is in the same branch. The phylogenetic tree of strain MR-43 is shown in FIG. 3.
The gene sequence of the strain MR-43 is shown as SEQ ID No. 1.
In combination with morphological, molecular biological identification, the strain MR-43 isolated from the root soil of Ledebouriella root was identified as S.castanedae.
Example 3
The example discloses a screening and soil colonisation ability evaluation test of a rifampicin (Rif) marker strain MR-43, which comprises the following specific steps:
1. rifampicin marker strain selection: rifampicin-resistant mutagenesis was performed on antagonistic fungus MR-43, and MR-43 was inoculated sequentially into PDB containing 50, 100, 200, 300, 350, 400. Mu.g/mL Rif and cultured stepwise under shaking culture at 25℃for 5d at 170 r/min. When the strain MR-43 can stably grow in the PDB containing 400 mug/mL of Rif and the morphological index and the antibacterial activity of the fusarium equisetum are not obviously changed, the rifampicin marker strain for antagonizing the fungus MR-43 is obtained, and the strain is numbered MRRif-43 and is preserved at the temperature of minus 20 ℃ for standby.
2. Bacterial strain MRRif-43 spore suspension preparation: after culturing MRRif-43 strain on PDA plate at 25deg.C for 7d, conidium is eluted with sterile water and formulated into 1×10 8 CFU/ml spore suspension, stored at 4℃for further use.
3. Soil ability evaluation of strain MR-43: uniformly mixing the tested MRRif-43 spore suspension and the divaricate saposhnikovia root cultivation soil according to the proportion of 1:10, putting the mixture into a seedling pot, repeating the treatment for 10 times, standing the mixture at room temperature, recovering and separating the strain MRRif-43 in the soil for 1 time every 7 days, and counting and recording the amount of colonial bacteria in the soil.
The result shows that the strain MRRif-43 can stably grow on a PDA plate containing 400 mu L/mg rifampicin after 10 passages of subculture, and the form of the strain MRRif-43 is not obviously changed compared with that of the strain MRRif-43; the bacterial strain MRRif-43 has no obvious difference on the antibacterial activity of fusarium oxysporum and fusarium equisetum compared with the bacterial strain MR-43, and the antibacterial rate can still be kept about 60%. The result shows that the strain MRRif-43 has genetic stability and still maintains good antibacterial activity on pathogenic fungi.
The soil colonization test period was 35d and the test soil without the labeled strain was tested and the microorganisms contained therein were not able to grow on PDA plates containing 400. Mu.g/mL rifampicin, thus demonstrating that antibiotic selection medium at this concentration was useful for efficient recovery of MRRif-43 strain. As shown in FIG. 4, the bacterial load change of the strain MRRif-43 in the soil has a remarkable fluctuation trend. Inoculating 14d, and reducing the soil bacterial content of MRRif-43 to 1.85 multiplied by 10 6 CFU/g soil; MRRif-43 realizes propagation, and the soil bacterial content of MRRif-43 is increased to 5.36 multiplied by 10 6 CFU/g soil, 190% amplification, significant difference (p<0.05 A) is provided; first, the28d, the colonization of the strain MRRif-43 showed a small decrease to 35d, and the soil bacterial content of the strain MRRif-43 was raised back to 5.38X10 6 CFU/g soil, significantly different from 28d (p<0.05 The amount of the colonisation bacteria is considerable. The results show that the strain MR-43 has good soil colonization ability.
Example 4
The embodiment discloses a disease prevention test of antagonistic fungi MR-43, which comprises the following steps:
preparation of pathogenic bacteria spore suspension: culturing Fusarium oxysporum and Fusarium equisetum strains on PDA plate at 25deg.C for 10d, eluting conidium of 2 pathogenic fungi with sterile water, and preparing into 1×10 respectively 8 CFU/ml spore suspension, stored at 4℃for further use.
Potted plant disease tests were performed with reference to the prior art (Gholami M, ami J, abmallahzadeh J, et al basic fungi as biocontrol agents against take-all disease of biological Control,2019, 130:34-43.) and were specifically: soil matrix (farmland soil: vermiculite=2:1) has been previously infected with fusarium oxysporum, fusarium equiseti spore suspensions, respectively. 1-year-old healthy windproof plants with consistent growth vigor and developed root systems are selected, 2 experimental communities are designed, namely fusarium oxysporum disease prevention areas respectively, 5 treatments are respectively arranged in each community, and the two areas are respectively: no-treatment control (water), pesticide treatment group (5.0 g/L carbendazim 50% wettable powder for preventing and curing fusarium oxysporum induced windbreak wilt, 0.18g/L mancozeb 70% wettable powder for preventing and curing fusarium induced windbreak root rot), bacillus subtilis suspension (with a bacterial content of 1×10) 7 CFU/mL), trichoderma harzianum spore suspension (bacterial content 1×10) 7 CFU/mL), MR-43 spore suspension (bacterial content 1×10) 7 CFU/mL)。
Each experimental cell was independently and completely randomly distributed, with 20 replicates per treatment. After the conventional agriculture management is carried out for 70 days, the disease condition of the windbreak wilt and the root rot is investigated, and the disease index and the disease prevention effect are calculated.
The onset of Fangfenghui wilt is classified as 9. Level 0: the plants are healthy and have no wilt symptom; stage 1: less than 10% of the plants yellow; 3 stages: 11% -25% of leaves wilt yellow and start sagging; 5 stages: 26 to 50 percent of leaves wither and turn yellow, and begin to fall off; 7 stages: the plant growth is obviously inhibited, the stalks are thin and sparse, 51 to 75 percent of leaves turn yellow, and a large amount of leaves fall off; stage 9: the whole plant withers to yellow, falls off as a whole and is seriously withered.
The onset of Ledebouriella root rot is classified as grade 9. Level 0: healthy plants, no disease spots; stage 1: less than 10% of the whole plant has disease; 3 stages: 11-25% of the whole plant leaves are ill; 5 stages: 26-50% of the whole plant leaves are ill; 7 stages: leaf disease of 51-75% of whole plant; stage 9: more than 76% of the whole plant has disease.
Disease index = [ Σ (number of disease-grade strains×representative value)/(total number of plants×highest disease-grade representative value) ]100
Disease preventing effect (%) = (control disease index-treatment disease index)/control disease index×100%
The results are shown in Table 2 and FIG. 5.
TABLE 2 control of wind-resistant fungal diseases by MR-43 spore suspensions
Note that: the different treatments in the table are respectively that A is MR-43, B is 50% carbendazim wettable powder or 70% mancozeb wettable powder, C is bacillus subtilis, D is trichoderma harzianum, and CK is clear water treatment.
As shown in Table 2, and figures 5 and 6, the spore suspension of the strain MR-43 can effectively relieve the disease occurrence of the wind-proof fusarium wilt and root rot after being applied back to the wind-proof cultivation soil, and reduce the disease hazard. After the MR-4370d inoculation, the control group divaricate saposhnikovia root plants have serious fusarium wilt and root rot, the disease indexes are respectively 50.59 and 64.14, and the divaricate saposhnikovia root diseases after the treatment of carbendazim and mancozeb pesticides and the treatment of bacillus subtilis, trichoderma harzianum and MR-43 spore suspension are controlled to different degrees. Wherein, the MR-43 has the prevention effect on the fusarium wilt and root rot of more than 63 percent, the average prevention effect is 68.51 percent, and the difference is not obvious (p is more than 0.05) compared with the pesticide treatment group; the MR-43 prevention effect of the strain is obviously higher than that of the bacillus subtilis and the Trichoderma harzianum.
Example 5
The embodiment discloses a test for evaluating the growth promoting ability of antagonistic fungi MR-43, which comprises the following steps:
soil substrates (farmland soil: vermiculite=2:1) have been foreseen for disinfection and sterilization. 1-year healthy windproof plants with consistent growth vigor and developed root systems are selected, and 4 treatments are set up, wherein the treatments are as follows: no-treatment control (water), bacillus subtilis suspension (bacterial content 1×10) 7 CFU/mL), trichoderma harzianum spore suspension (bacterial content 1×10) 7 CFU/mL), MR-43 spore suspension (bacterial content 1×10) 7 CFU/mL). The experimental cells were completely randomly distributed, with 15 replicates per treatment. After 60 days of conventional agricultural management, 9 divaricate saposhnikovia root plants are randomly extracted, and biomass indexes such as the length, the root length, the fresh weight, the dry weight, the fresh weight and the dry weight of the whole divaricate saposhnikovia root are measured and recorded.
The results are shown in Table 3.
Table 3 MR-43 effects on the promotion of Ledebouriella plants
| Different treatments | Length/cm of whole plant | Root length/cm | Fresh weight/g of whole plant | Fresh weight/g of root | Whole plant dry weight/g | Dry weight of root/g |
| A | 55.73±3.52a | 29.44±3.42a | 8.21±0.40a | 3.88±0.51a | 2.56±0.67a | 1.32±0.30a |
| B | 51.63±3.58b | 29.34±2.15a | 7.66±1.62a | 3.04±0.47b | 2.49±0.97a | 1.04±0.30b |
| C | 51.35±4.14b | 28.63±2.84a | 7.89±1.98a | 3.10±0.39b | 2.54±1.01a | 1.06±0.25b |
| CK | 50.85±3.21b | 28.11±2.03a | 6.44±1.37b | 2.28±0.81c | 1.92±0.60b | 0.85±0.18c |
Note that: each column in the table was analyzed by comparison based on the new complex polar difference method of duncan's. The different treatments are MR-43, B, C, trichoderma harzianum, CK and clear water treatment.
After the strain MR-43 is inoculated on the divaricate saposhnikovia root, stem, root and whisker and other parts of the divaricate saposhnikovia root, the strain MR-43 does not have mildew or disease spots caused by pathogenicity, which indicates that the S.castanedae MR-43 has no pathogenicity on the divaricate saposhnikovia root of the medicinal plant.
As shown in Table 3, the strain MR-43, B.subtilis bacterial suspension and T.harzianum spore suspension all promoted growth of Ledebouriella plants to different degrees. The indexes of the whole length, the fresh weight and the dry weight of the roots of the divaricate saposhnikovia root treated by the MR-43 are obviously higher than those of other treatment groups (p < 0.05); compared with the CK group (clear water treatment), 6 indexes such as the whole length, the whole fresh weight and the like of the radix sileris in the MR-43 group are increased by 33.44 percent on average, and the fresh weight and the dry weight of the radix sileris roots are increased by more than 44 percent. The result shows that the strain MR-43 can promote the growth of divaricate saposhnikovia root and has the attribute of Plant growth-promoting fungi (PGPF).
Finally, it should be noted that: the above embodiments are merely preferred embodiments of the present invention for illustrating the technical solution of the present invention, but not limiting the scope of the present invention; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the corresponding technical solutions; that is, even though the main design concept and spirit of the present invention is modified or finished in an insubstantial manner, the technical problem solved by the present invention is still consistent with the present invention, and all the technical problems are included in the protection scope of the present invention; in addition, the technical scheme of the invention is directly or indirectly applied to other related technical fields, and the technical scheme is included in the scope of the invention.
Claims (8)
1. A fungus of the family scilliaceae, characterized in that it has a deposit number of: GDMCC No.62081.
2. According to claim 1The application of the fungus or spore suspension of the fungus in the family of the grape spike mould in preparing the medicine for preventing and controlling the pathogenic fungi of the radix sileris; the radix Saposhnikoviae pathogenic fungi is Fusarium oxysporumFusarium oxysporumFusarium equisetumF. equisetiAt least one of them.
3. The use according to claim 2, wherein the medicament for controlling pathogenic fungi of saposhnikovia is applied to the soil of saposhnikovia cultivation.
4. The preparation of fusarium oxysporum control of the species fusarium oxysporum, which is a suspension of a fungus of the family sciences scirpus or spores thereof of claim 1Fusarium oxysporumDrug for preventing wind-proof wilt and/or fusarium equisetum caused by the sameF. equisetiApplication of the medicine for preventing and treating wind root rot caused by the wind root rot is provided.
5. Use of a fungus of the family scirpaceae or a spore suspension thereof according to claim 1 for the preparation of a formulation for promoting growth of a ledebouriella plant.
6. The use according to claim 4 or 5, wherein the agent for controlling windbreak wilt, the agent for controlling windbreak root rot and the agent for promoting growth of windbreak plants are applied to the windbreak cultivation soil.
7. The use according to any one of claims 2 to 5, wherein the spore suspension has a bacterial content of 1 x 10 6 ~ 1×10 8 CFU/mL。
8. The use according to claim 7, wherein the spore suspension has a bacterial content of 1 x 10 7 CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210990293.7A CN116333888B (en) | 2022-07-21 | 2022-07-21 | Fungus of family Botrytis and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210990293.7A CN116333888B (en) | 2022-07-21 | 2022-07-21 | Fungus of family Botrytis and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116333888A CN116333888A (en) | 2023-06-27 |
| CN116333888B true CN116333888B (en) | 2024-03-01 |
Family
ID=86890361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210990293.7A Active CN116333888B (en) | 2022-07-21 | 2022-07-21 | Fungus of family Botrytis and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116333888B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134053A (en) * | 2021-12-23 | 2022-03-04 | 吉林农业大学 | Aspergillus ascomycete MR-86 and application thereof |
| CN114196553A (en) * | 2022-01-14 | 2022-03-18 | 吉林农业大学 | Aureobasidium sp MR-57 and application thereof |
| CN114574369A (en) * | 2022-03-08 | 2022-06-03 | 吉林农业大学 | Acremonium persicinum MR-47 and application thereof |
-
2022
- 2022-07-21 CN CN202210990293.7A patent/CN116333888B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134053A (en) * | 2021-12-23 | 2022-03-04 | 吉林农业大学 | Aspergillus ascomycete MR-86 and application thereof |
| CN114196553A (en) * | 2022-01-14 | 2022-03-18 | 吉林农业大学 | Aureobasidium sp MR-57 and application thereof |
| CN114574369A (en) * | 2022-03-08 | 2022-06-03 | 吉林农业大学 | Acremonium persicinum MR-47 and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 5.8S ribosomal RNA gene, complete sequence * |
| and internal transcribed spacer 2, partial sequence.《Genbank Database》.2021,OK287148.1. * |
| Wang,Y..Sirastachys castanedae strain MR43 internal transcribed spacer 1, partial sequence * |
| 河南山坡土壤中三种葡萄穗霉科真菌鉴定;翟妮平等;《土壤通报》;第50卷(第04期);第878-884页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116333888A (en) | 2023-06-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110066756B (en) | Paenibacillus kribbensis and preparation and application thereof | |
| CN107136122B (en) | Biocontrol microbial inoculum for preventing and treating potato late blight | |
| CN103131656B (en) | Streptomyces YT027 and application thereof | |
| CN112812971B (en) | Agrocybe aegerita symbiotic fungus M2-1, and microbial inoculum and fermentation broth extract thereof | |
| CN114574369B (en) | Acremonium persicinum MR-47 and application thereof | |
| CN112342173A (en) | Bacillus belgii and application thereof | |
| CN113444651B (en) | Saffron endophytic fungus and application thereof in preventing and treating bulb rot | |
| CN116987615B (en) | Lecaniella albopictus YZ-151 and application thereof | |
| CN111378595B (en) | Burkholderia agricultural biocontrol strain Ba1 and application thereof | |
| CN114836329B (en) | Trichoderma harzianum HB40609 and application thereof | |
| CN115197853B (en) | Endophyte Epicoccum thailandicum LF-28 strain and application thereof | |
| CN116333888B (en) | Fungus of family Botrytis and application | |
| CN114395486B (en) | Adhesive film fungus strain TP-3 with high growth promoting capability of dendrobium and application thereof | |
| CN114507618B (en) | Oncomelania mycorrhizal fungus strain TP-11 with dendrobium new leaf growth promoting capability and application thereof | |
| CN114032182B (en) | Fungus with functions of antagonizing pathogenic bacteria of garlic root rot and promoting growth | |
| HASSAN et al. | Molecular identification and biomass production of an endophytic Beauveria bassiana isolated from cucumber leaves in Iraq | |
| CN110607249B (en) | Streptomyces biocontrol strain and application thereof | |
| Ahmed et al. | Isolation and identification of rhizosphere soil fungi from Papaya (Carica papaya L.) and eggplant (Solanum melongena L.) at BCSIR Campus in Rajshahi, Bangladesh | |
| CN114774291B (en) | Trichoderma asperellum 10564 strain and application thereof in biocontrol of tomato fusarium wilt | |
| CN116042492B (en) | Bacillus pumilus and application thereof in plant disease control | |
| CN116925950B (en) | Biological control strain for pepper leaf spot disease and application thereof | |
| CN116286536B (en) | Bacillus cereus YZ-228 and application thereof | |
| LU502326B1 (en) | Isaria javanica strain for preventing and treating citrus pests and use thereof | |
| CN113512502B (en) | Endophytic fungus and application thereof in preventing and treating rot | |
| CN114672424B (en) | Tea tree endophyte and application thereof in plant disease control |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |