CN116333872A - Microorganism detection device and detection method thereof - Google Patents

Microorganism detection device and detection method thereof Download PDF

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Publication number
CN116333872A
CN116333872A CN202310261159.8A CN202310261159A CN116333872A CN 116333872 A CN116333872 A CN 116333872A CN 202310261159 A CN202310261159 A CN 202310261159A CN 116333872 A CN116333872 A CN 116333872A
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sample
detection
bevel gear
oil
adjusting
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CN116333872B (en
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董跃明
刘昕
马楠
贾婧雯
潘红芳
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8th Medical Center of PLA General Hospital
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8th Medical Center of PLA General Hospital
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106General arrangement of respective parts
    • G01N2021/0112Apparatus in one mechanical, optical or electronic block
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
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Abstract

The invention discloses a microorganism detection device and a detection method thereof, belonging to the technical field of microorganism detection, and comprising a shell, wherein an organic door is arranged outside the shell through a hinge, an observation mirror is fixedly arranged on the top surface of the shell, a detector is fixedly arranged at the top end of the observation mirror through a mounting frame, an adjusting component is arranged inside the shell, two polishing components are arranged on the adjusting component and used for polishing in the microorganism detection process, and a detection sample placing mechanism is arranged on the inner wall of the bottom surface of the detector; according to the invention, the adjusting component, the polishing component and the detection sample placement mechanism are arranged in a matching way, so that the position of the sample can be quickly adjusted according to the requirements on detection and observation of microorganisms, and the position of the light source can be synchronously adjusted, so that the sample can be in a more excellent detection and observation state without additional operation, the operation is convenient and quick, and the convenience and the efficiency of equipment use are greatly improved.

Description

Microorganism detection device and detection method thereof
Technical Field
The invention belongs to the technical field of microorganism detection, and particularly relates to a microorganism detection device and a microorganism detection method.
Background
Microorganisms, as their name implies, are tiny organisms, which generally include: bacteria, viruses, fungi and some small protozoa, microalgae, etc., and its individual is tiny and closely related to human. The method covers various harmful substances, and is widely applied to various fields of food, medicine, industry and agriculture, environmental protection, sports and the like, and a microorganism detection device is needed when the microorganism in a sample is required to be detected.
The existing microorganism detection device generally introduces a certain special detection reagent into a detection sample, and then observes the sample through an observation device, so that the quantity and distribution of microorganisms can be detected, but the device can have a certain observation problem when in use, when in near-point observation of a certain microorganism, the environment light supplementing is needed, so that the near-point microorganism is observed more carefully, when in observation detection of the microorganism in a certain area, the light source position needs to be regulated, the light source is dispersed, so that a more comprehensive observation effect is acquired, the light source needs to be regulated timely, and the position of the microorganism sample needs to be regulated simultaneously, so that a better detection observation effect can be realized.
Disclosure of Invention
The invention aims at: in order to solve the problems that the existing microorganism detection device generally introduces a certain special detection reagent into a detection sample, and then observes the sample through an observation device, so that the quantity and distribution of microorganisms can be detected, but the device can be used for detecting the microorganisms, and when the microorganisms are observed in a near point, the environment light supplementing is needed, so that the near point microorganisms are observed more carefully, and when the microorganisms in a certain area are observed and detected, the position of a light source is needed to be regulated, so that the light source is dispersed, so that a more comprehensive observation effect is obtained, the light source is needed to be regulated timely, and the positions of the microorganism samples are also regulated simultaneously, so that the better detection and observation effect can be realized, the operation is complex, and the overall detection efficiency is low.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the utility model provides a microorganism detection device, includes the casing, the outside of casing is through hinge installation organic door, fixed mounting has the sight glass on the top surface of casing, there is the detection machine at the top of sight glass through mounting bracket fixed mounting, the inside of casing is provided with adjusting part, be provided with two components of polishing on the adjusting part, the component of polishing is used for the polishing of microorganism detection in-process, be provided with detection sample placement machine on the bottom surface inner wall of detection machine, detection sample placement machine is used for the stable placement of detection sample.
As a further description of the above technical solution:
the polishing assembly comprises a movable seat, the movable seat is slidably mounted on the inner wall of the bottom surface of the detection machine, an installation guard plate is fixedly mounted on the top surface of the movable seat through an installation rod, a groove is formed in the outer surface of the installation guard plate, an arc-shaped light plate is mounted in the groove in an embedded mode, and a pressing sheet is fixedly mounted on the outer wall of one side of the movable seat through a connecting rod.
As a further description of the above technical solution:
the adjusting assembly comprises an adjusting motor, the adjusting motor is fixedly arranged on the outer wall of one side of the shell, a bidirectional stud is fixedly arranged at one end of an output shaft of the adjusting motor, a transmission bevel gear is fixedly arranged at the middle position of the bidirectional stud, and the bidirectional stud is in threaded connection with a threaded hole formed in the movable seat.
As a further description of the above technical solution:
the detection sample placement mechanism comprises an adjusting stud and a sliding rod, the adjusting stud is rotatably mounted on the inner wall of the bottom surface of the detection machine, the sliding rod is fixedly mounted on the inner wall of the bottom surface of the detection machine, and a base is slidably mounted outside the sliding rod.
As a further description of the above technical solution:
the base is in threaded connection with the adjusting stud through a threaded hole formed in the bottom of the base, a driven bevel gear is fixedly arranged outside the adjusting stud, the driving bevel gear is connected with the driven bevel gear in a meshed mode, a placing table is fixedly arranged at the top of the base, a travel groove is formed in the top surface of the placing table, and the central shaft of the observation mirror coincides with the central shaft of the placing table.
As a further description of the above technical solution:
the inside of travel groove is provided with positioning assembly, positioning assembly includes the slide shaft, the longitudinal fixed mounting of slide shaft is on the bottom surface inner wall of placing the platform, the outside cover of slide shaft is equipped with the suit spring.
As a further description of the above technical solution:
the outside slidable mounting of slide shaft has the depression bar, equal fixed mounting has the kelly on the both sides outer wall of depression bar, the inside slidable mounting of travel groove has the slider, the top fixed mounting of slider has the arc locating plate.
As a further description of the above technical solution:
the spring shaft is fixedly installed on the outer wall of one side of the sliding block, one end of the spring shaft is fixedly connected with the inner wall of one side of the travel groove, one end of the clamping rod is clamped into the clamping hole arranged on the bottom surface of the sliding block, and the top end of the compression rod penetrates through and extends to the outer side of the placing table.
As a further description of the above technical solution:
the oil storage assembly is used for storing and guiding out lubricating oil and is located between the two polishing assemblies, the oil storage assembly comprises an oil box, an oil bag is arranged in the oil box, an oil pipe is fixedly installed on the side wall of the oil bag, one end of the oil pipe penetrates through and extends to the outer side of the oil box, an oil injection head is fixedly installed at one end of the oil pipe, and one end of the oil injection head is in tight contact with a transmission bevel gear.
The invention also discloses a detection method of the microorganism detection device, which comprises the following steps:
s1, when a sample needs to be placed and positioned, the sample is directly placed in the center of a sample detection placing mechanism, a pressing rod is pressed down at the moment, a clamping rod is synchronously lowered, the top end of the clamping rod is separated from a clamping hole at the bottom of a sliding block, a spring shaft laterally bounces an arc-shaped positioning plate at the moment, the other arc-shaped positioning plate is synchronously triggered, and the two arc-shaped positioning plates simultaneously position the sample;
s2, when the microorganism detection is carried out, a detection sample added with a detection reagent is placed on a detection sample placement mechanism, during the detection, a detection sample can be carefully observed by an observation mirror, and an observed image can be displayed in a picture of a detector in real time;
s3, if the sample microorganisms need to be closely pulled to be carefully observed, an adjusting motor of an adjusting assembly is started, the adjusting motor can control a two-way screw bolt to rotate, a transmission bevel gear can synchronously rotate, the transmission bevel gear drives the adjusting screw bolt with a driven bevel gear to rotate through meshing action, the adjusting screw bolt can control a placing table to be lifted, at the moment, the distance between the sample placed on the placing table and an observation mirror is shortened, close-range observation can be carried out, meanwhile, when the two-way screw bolt rotates, two movable seats on the placing table can be driven to be mutually close, at the moment, two arc-shaped light plates are gradually close to the sample, so that more effective light supplementing can be given to the sample, and the carefully observed effect on the microorganisms in the sample is further improved;
s4, if regional observation is carried out relative to microorganisms in the sample, controlling and adjusting stud inversion, automatically falling the height of the sample placing mechanism, and keeping the two arc-shaped light plates away, so that the sample is subjected to more divergent illumination, and the sample is widely and comprehensively observed;
s5, when the position of the polishing assembly and the position of the detection sample placement mechanism are adjusted, the pressing sheet can synchronously move sideways, at the moment, the pressing sheet can extrude oil bags in the oil storage assembly, oil in the extruded oil bags can be guided onto the driven bevel gear through the oil injection head, and the oil on the driven bevel gear can be transmitted onto the transmission bevel gear through meshing transmission, so that effective lubrication of the driven bevel gear and the transmission bevel gear is realized, friction between the driven bevel gear and the transmission bevel gear is reduced, and transmission efficiency and smoothness of the driven bevel gear and the transmission bevel gear are improved.
In summary, due to the adoption of the technical scheme, the beneficial effects of the invention are as follows:
1. according to the invention, the adjusting component, the polishing component and the detection sample placement mechanism are arranged in a matching way, when the microorganism detection is carried out, the detection sample added with the detection reagent is placed on the detection sample placement mechanism, during the detection, the observation mirror can carry out careful observation on the detection sample, the observed image can be displayed in the picture of the detection machine in real time, if the microorganism in the sample is required to be zoomed in for careful observation, the adjusting motor of the adjusting component is started, the adjusting motor can control the bidirectional stud to rotate, the transmission bevel gear can synchronously rotate, the transmission bevel gear drives the adjusting stud with the driven bevel gear to rotate through the meshing effect, the adjusting stud can control the placement table to lift, at the moment, the distance between the sample placed on the placement table and the observation mirror is shortened, the two movable seats on the bidirectional stud can be driven to be mutually close, at the moment, the two arc-shaped light plates are gradually close to the sample, so that the sample can be more effectively supplemented with light, and the fine observation effect on the sample is further improved, otherwise, the adjusting stud is controlled to rotate, the sample placement mechanism can automatically fall down, the speed is high, the two arc-shaped light plates can be conveniently and rapidly observed, the sample can be conveniently and rapidly arranged, the operation is required to be conveniently and rapidly arranged, and rapidly, and conveniently can be observed according to the requirements of the detection position is realized.
2. According to the invention, the positioning assembly is arranged in the detection sample placement mechanism in a matched manner, when a sample is required to be placed and positioned, the sample is directly placed in the center of the detection sample placement mechanism, the compression bar is pressed down at the moment, the clamping bar is synchronously lowered, the top end of the clamping bar can be separated from the clamping hole at the bottom of the sliding block, the arc-shaped positioning plate is laterally sprung by the spring shaft at the moment, the other arc-shaped positioning plate is synchronously triggered, and the two arc-shaped positioning plates simultaneously position the sample.
3. According to the invention, the oil storage assembly is arranged in a matching way, when the positions of the polishing assembly and the detection sample placement mechanism are adjusted, the pressing sheet can synchronously move sideways, at the moment, the pressing sheet can extrude the oil bag in the oil storage assembly, the oil in the extruded oil bag can be led to the driven bevel gear through the oil injection head, the oil on the driven bevel gear can be transmitted to the transmission bevel gear through meshing transmission, so that effective lubrication of the driven bevel gear and the transmission bevel gear is realized, the friction force between the driven bevel gear and the transmission bevel gear is reduced, the transmission efficiency and smoothness of the driven bevel gear and the transmission bevel gear are improved, long-time normal use of equipment is ensured, manual inspection is not needed, and the use effect is good.
Drawings
Fig. 1 is a schematic perspective view of a microorganism detection apparatus.
Fig. 2 is an exploded perspective view of a microorganism detecting apparatus.
Fig. 3 is an enlarged exploded perspective view of an adjusting assembly and a sample placement mechanism in a microorganism detecting apparatus.
Fig. 4 is an enlarged exploded perspective view of an adjusting assembly and a polishing assembly in a microorganism detecting apparatus.
FIG. 5 is an enlarged exploded perspective view of a sample placement mechanism for a microorganism detection apparatus.
FIG. 6 is an enlarged exploded view of a positioning assembly of a microbiological detection device.
Fig. 7 is an enlarged perspective view of an oil storage assembly of the microorganism detecting apparatus.
FIG. 8 is an enlarged schematic view of the structure of the microorganism detecting apparatus at A.
Legend description:
1. a gate; 2. a detecting machine; 3. an observation mirror; 4. a housing; 5. an adjustment assembly; 51. a two-way stud; 52. adjusting a motor; 53. a drive bevel gear; 6. an oil storage assembly; 61. an oil spray head; 62. an oil box; 7. a polishing assembly; 71. an arc-shaped light plate; 72. mounting a guard board; 73. a movable seat; 74. tabletting; 8. detecting a sample placement mechanism; 81. a driven bevel gear; 82. adjusting the stud; 83. a slide bar; 84. a base; 85. a placement table; 86. a travel groove; 87. a positioning assembly; 871. an arc-shaped positioning plate; 872. a slide block; 873. a spring shaft; 874. a compression bar; 875. a clamping rod; 876. sleeving a spring; 877. a sliding shaft.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-8, the present invention provides a technical solution: the utility model provides a microorganism detection device, includes casing 4, the outside of casing 4 is through hinge installation machine door 1, fixed mounting has sight glass 3 on the top surface of casing 4, the top of sight glass 3 is through mounting bracket fixed mounting has detection machine 2, the inside of casing 4 is provided with adjusting part 5, be provided with two subassembly 7 of polishing on the adjusting part 5, the subassembly 7 of polishing is used for the polishing of microorganism detection in-process, be provided with detection sample on the bottom surface inner wall of detection machine 2 and place mechanism 8, detection sample places mechanism 8 and is used for the stable placement of detection sample.
The polishing assembly 7 comprises a movable seat 73, the movable seat 73 is slidably mounted on the inner wall of the bottom surface of the detecting machine 2, an installation guard plate 72 is fixedly mounted on the top surface of the movable seat 73 through an installation rod, a groove is formed in the outer surface of the installation guard plate 72, an arc-shaped light plate 71 is mounted in the groove in an embedded mode, and a pressing sheet 74 is fixedly mounted on the outer wall of one side of the movable seat 73 through a connecting rod.
The adjusting assembly 5 comprises an adjusting motor 52, the adjusting motor 52 is fixedly arranged on the outer wall of one side of the casing 4, one end of an output shaft of the adjusting motor 52 is fixedly provided with a bidirectional stud 51, the middle position of the bidirectional stud 51 is fixedly provided with a transmission bevel gear 53, and the bidirectional stud 51 is in threaded connection with a threaded hole formed in the movable seat 73.
The detection sample placement mechanism 8 comprises an adjusting stud 82 and a sliding rod 83, the adjusting stud 82 is rotatably mounted on the inner wall of the bottom surface of the detection machine 2, the sliding rod 83 is fixedly mounted on the inner wall of the bottom surface of the detection machine 2, a base 84 is slidably mounted on the outer portion of the sliding rod 83, the base 84 is in threaded connection with the adjusting stud 82 through a threaded hole formed in the bottom of the base 84, a driven bevel gear 81 is fixedly mounted on the outer portion of the adjusting stud 82, the driving bevel gear 53 and the driven bevel gear 81 are in meshed connection with each other, a placement table 85 is fixedly mounted on the top of the base 84, a travel groove 86 is formed in the top surface of the placement table 85, and the central axis of the observation mirror 3 coincides with the central axis of the placement table 85.
The specific implementation mode is as follows: when the microorganism detection is carried out, the detection sample added with the detection reagent is placed on the detection sample placement mechanism 8, during detection, the observation mirror 3 can carry out careful observation on the detection sample, an observed image can be displayed in a picture of the detection machine 2 in real time, if the sample microorganism needs to be zoomed in and carefully observed, the adjusting motor 52 of the adjusting component 5 is started, the adjusting motor 52 can control the bidirectional stud 51 to rotate, the transmission bevel gear 53 can synchronously rotate, the transmission bevel gear 53 drives the adjusting stud 82 with the driven bevel gear 81 to rotate through meshing action, the adjusting stud 82 can control the placement table 85 to lift, at the moment, the distance between the sample placed on the placement table 85 and the observation mirror 3 is zoomed in, close-range observation can be carried out, meanwhile, the two movable seats 73 on the bidirectional stud 51 can be driven to be mutually close, at the moment, the two arc-shaped light plates 71 are gradually close to the sample, so that more effective light supplementing can be given to the sample, and further improve the careful observation effect on the microorganism in the sample, otherwise, the adjustment stud 82 is controlled to rotate in a region relative to the microorganism in the sample, the sample can automatically fall down to the height of the detection sample placement mechanism 8, the two arc-shaped light plates can be more widely spread and the sample can be more widely observed.
Through this design, can be according to the demand to microorganism detection observation, the position of quick adjustment sample can the synchronous adjustment light source's position simultaneously to can make the sample be in under the more excellent detection observation state, need not extra operation, convenient operation is swift, has improved the convenience and the efficiency of equipment use greatly.
In the detection of microorganisms, the slide or the culture medium is a wet object, which easily causes rust between the transmission members. The bevel gears (driven bevel gear 81 and drive bevel gear 53) are in a relatively open environment, and the bevel gears require high precision to ensure that the distance of the light source and the height of the placement table 85 are within a reliable relationship. Based on this, as a further solution of the present application, there is a need to solve the lubrication problem between bevel gears:
the inside of travel slot 86 is provided with locating component 87, locating component 87 includes slide shaft 877, slide shaft 877 vertical fixed mounting is on the bottom surface inner wall of placing table 85, slide shaft 877's outside cover is equipped with the suit spring 876, slide shaft 877's outside slidable mounting has depression bar 874, all fixed mounting has clamping lever 875 on the both sides outer wall of depression bar 874, travel slot 86's inside slidable mounting has slider 872, the top fixed mounting of slider 872 has arc locating plate 871, fixed mounting has spring shaft 873 on the outer wall of one side of slider 872, one end of spring shaft 873 and one side inner wall fixed connection of travel slot 86, the one end block of clamping lever 875 is inlayed to the card downthehole that sets up on the bottom surface of slider 872, the top of depression bar 874 passes and extends to the outside of placing table 85.
The specific implementation mode is as follows: when adjusting the position of polishing subassembly 7 and detection sample placement machine 8, preforming 74 can move side in step, preforming 74 can produce the extrusion to the oil pocket in the oil storage subassembly 6 this moment, receive the inside fluid accessible injection head 61 of extruded oil pocket to guide into on the driven bevel gear 81, the fluid accessible meshing transmission on the driven bevel gear 81 is conducted to on the transmission bevel gear 53, realize the effective lubrication of two, thereby reduce the frictional force between the two, improve driven bevel gear 81 and transmission bevel gear 53's transmission efficiency and smoothness, guarantee the long-time normal use of equipment, need not regularly to carry out manual inspection, excellent in use effect.
The oil storage component 6 is arranged on the inner wall of the bottom surface of the shell 4, the oil storage component 6 is used for storing and guiding out lubricating oil, the oil storage component 6 is positioned between the two polishing components 7, the oil storage component 6 comprises an oil box 62, an oil bag is arranged in the oil box 62, an oil pipe is fixedly arranged on the side wall of the oil bag, one end of the oil pipe penetrates through and extends to the outer side of the oil box 62, an oil injection head 61 is fixedly arranged at one end of the oil pipe, and one end of the oil injection head 61 is in close contact with the transmission bevel gear 53.
The specific implementation mode is as follows: when the sample needs to be placed and positioned, the sample is directly placed at the center of the sample placement mechanism 8, at the moment, the pressing rod 874 is pressed to descend, the clamping rod 875 descends synchronously, the top end of the clamping rod 875 can be separated from the clamping hole at the bottom of the sliding block 872, at the moment, the spring shaft 873 bounces the arc-shaped positioning plate 871 side, the other arc-shaped positioning plate 871 triggers synchronously, and the two arc-shaped positioning plates 871 simultaneously position the sample.
Through this design, can be when the sample is placed to the sample location, need not unnecessary operation, further improve the convenience when the device operation, improve device result of use.
The invention also discloses a detection method of the microorganism detection device, which comprises the following steps:
s1, when a sample needs to be placed and positioned, the sample is directly placed in the center of a detection sample placing mechanism 8, at the moment, a pressing rod 874 is pressed to descend, a clamping rod 875 descends synchronously, the top end of the clamping rod 875 is separated from a clamping hole at the bottom of a sliding block 872, at the moment, a spring shaft 873 bounces the side of an arc-shaped positioning plate 871, the other arc-shaped positioning plate 871 triggers synchronously, and the two arc-shaped positioning plates 871 simultaneously position the sample;
s2, when the microorganism detection is carried out, a detection sample added with a detection reagent is placed on the detection sample placement mechanism 8, during the detection, the observation mirror 3 can carry out fine observation on the detection sample, and an observed image can be displayed in a picture of the detector 2 in real time;
s3, if the sample microorganisms need to be closely and carefully observed, starting an adjusting motor 52 of the adjusting assembly 5, wherein the adjusting motor 52 can control a two-way stud 51 to rotate, a transmission bevel gear 53 can synchronously rotate, the transmission bevel gear 53 drives an adjusting stud 82 with a driven bevel gear 81 to rotate through meshing, the adjusting stud 82 can control a placing table 85 to be lifted, at the moment, the distance between the sample placed on the placing table 85 and an observation mirror 3 is shortened, close-range observation can be performed, at the same time, when the two-way stud 51 rotates, two movable seats 73 on the two-way stud 51 can be driven to be mutually close, at the moment, two arc-shaped light plates 71 are gradually close to the sample, so that more effective light supplementing can be given to the sample, and the fine observation effect on the microorganisms in the sample can be further improved;
s4, if regional observation is carried out relative to microorganisms in the sample, the adjusting stud 82 is controlled to rotate reversely, the sample placing mechanism 8 automatically drops to a height, and the two arc-shaped light plates 71 are far away, so that more divergent illumination is given to the sample, and the sample is widely and comprehensively observed;
s5, when the positions of the polishing assembly 7 and the detection sample placement mechanism 8 are adjusted, the pressing sheet 74 can synchronously move sideways, at the moment, the pressing sheet 74 can squeeze oil bags in the oil storage assembly 6, oil in the squeezed oil bags can be led into the driven bevel gear 81 through the oil injection head 61, and the oil on the driven bevel gear 81 can be transmitted to the transmission bevel gear 53 through meshing transmission, so that effective lubrication of the driven bevel gear 81 and the transmission bevel gear 53 is realized, friction between the driven bevel gear 81 and the transmission bevel gear 53 is reduced, and transmission efficiency and smoothness of the driven bevel gear are improved.
Working principle: when the sample needs to be placed and positioned, the sample is directly placed in the center of the sample placing mechanism 8, at the moment, the pressure lever 874 is pressed to descend, the clamping rod 875 descends synchronously, the top end of the clamping rod 875 is separated from the clamping hole at the bottom of the sliding block 872, at the moment, the spring shaft 873 bounces the arc-shaped positioning plate 871 side, the other arc-shaped positioning plate 871 triggers synchronously, and the two arc-shaped positioning plates 871 simultaneously position the sample; when the microorganism detection is carried out, a detection sample added with a detection reagent is placed on the detection sample placement mechanism 8, and during the detection, the observation mirror 3 can finely observe the detection sample, and an observed image can be displayed in a picture of the detector 2 in real time; if the sample microorganism needs to be closely and carefully observed, the adjusting motor 52 of the adjusting assembly 5 is started, the adjusting motor 52 can control the bidirectional stud 51 to rotate, the transmission bevel gear 53 can synchronously rotate, the transmission bevel gear 53 drives the adjusting stud 82 with the driven bevel gear 81 to rotate through meshing action, the adjusting stud 82 can control the placing table 85 to be lifted, at the moment, the distance between the sample placed on the placing table 85 and the observation mirror 3 is shortened, close-range observation can be performed, meanwhile, when the bidirectional stud 51 rotates, the two moving seats 73 on the bidirectional stud 51 can be driven to be mutually close, at the moment, the two arc-shaped light plates 71 are gradually close to the sample, so that more effective light supplementing can be given to the sample, and the fine observation effect on the microorganism in the sample is further improved; if the regional observation is performed relative to microorganisms in the sample, the control adjusting stud 82 is controlled to rotate reversely, the sample placing mechanism 8 automatically drops to a height, and the two arc-shaped light plates 71 are far away, so that the sample is subjected to more divergent illumination, and the sample is widely and comprehensively observed; when adjusting the position of the polishing assembly 7 and detecting the sample placement mechanism 8, the preforming 74 can synchronously move sideways, at this moment, the preforming 74 can extrude the oil pocket in the oil storage assembly 6, the oil in the extruded oil pocket can be led into the driven bevel gear 81 through the oil injection head 61, the oil on the driven bevel gear 81 can be transmitted to the transmission bevel gear 53 through meshing transmission, effective lubrication of the driven bevel gear 81 and the transmission bevel gear 53 is realized, friction force between the driven bevel gear 81 and the transmission bevel gear 53 is reduced, and transmission efficiency and smoothness of the driven bevel gear 81 and the transmission bevel gear 53 are improved.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.

Claims (10)

1. A microorganism detection apparatus comprising a housing (4), characterized in that: the outside of casing (4) is installed through the hinge and is taken turns door (1), fixed mounting has observation mirror (3) on the top surface of casing (4), the top of observation mirror (3) is passed through mounting bracket fixed mounting and is had detection machine (2), the inside of casing (4) is provided with adjusting part (5), be provided with two on adjusting part (5) and polish subassembly (7), polish subassembly (7) are used for the polishing of microorganism detection in-process, be provided with detection sample placement machine (8) on the bottom surface inner wall of detection machine (2), detection sample placement machine (8) are used for the stable placement of detection sample.
2. The microorganism detection apparatus according to claim 1, wherein the polishing assembly (7) comprises a movable seat (73), the movable seat (73) is slidably mounted on the inner wall of the bottom surface of the detector (2), a mounting guard plate (72) is fixedly mounted on the top surface of the movable seat (73) through a mounting rod, a groove is formed in the outer surface of the mounting guard plate (72), an arc-shaped light plate (71) is mounted in the groove in an embedded manner, and a pressing sheet (74) is fixedly mounted on the outer wall of one side of the movable seat (73) through a connecting rod.
3. The microorganism detection apparatus according to claim 2, wherein the adjusting assembly (5) comprises an adjusting motor (52), the adjusting motor (52) is fixedly installed on an outer wall of one side of the casing (4), a bidirectional stud (51) is fixedly installed at one end of an output shaft of the adjusting motor (52), a transmission bevel gear (53) is fixedly installed at a middle position of the bidirectional stud (51), and the bidirectional stud (51) is in threaded connection with a threaded hole formed in the movable seat (73).
4. A microbiological detection device according to claim 3, wherein the detection sample placement mechanism (8) comprises an adjusting stud (82) and a sliding rod (83), the adjusting stud (82) is rotatably mounted on the inner wall of the bottom surface of the detection machine (2), the sliding rod (83) is fixedly mounted on the inner wall of the bottom surface of the detection machine (2), and a base (84) is slidably mounted on the outer part of the sliding rod (83).
5. The microorganism detection apparatus according to claim 4, wherein the base (84) is in threaded connection with the adjusting stud (82) through a threaded hole formed in the bottom of the base, a driven bevel gear (81) is fixedly mounted on the outer portion of the adjusting stud (82), the driving bevel gear (53) and the driven bevel gear (81) are in meshed connection with each other, a placement table (85) is fixedly mounted on the top of the base (84), a travel groove (86) is formed in the top surface of the placement table (85), and the central axis of the observation mirror (3) coincides with the central axis of the placement table (85).
6. The microorganism detection apparatus according to claim 5, wherein a positioning assembly (87) is provided inside the travel groove (86), the positioning assembly (87) comprises a sliding shaft (877), the sliding shaft (877) is longitudinally and fixedly installed on the inner wall of the bottom surface of the placement table (85), and a sleeving spring (876) is sleeved outside the sliding shaft (877).
7. The microorganism detection apparatus according to claim 6, wherein a pressing rod (874) is slidably mounted on the outer side of the sliding shaft (877), clamping rods (875) are fixedly mounted on the outer walls of both sides of the pressing rod (874), a sliding block (872) is slidably mounted in the travel groove (86), and an arc-shaped positioning plate (871) is fixedly mounted at the top end of the sliding block (872).
8. The microorganism detection apparatus according to claim 7, wherein a spring shaft (873) is fixedly installed on an outer wall of one side of the slider (872), one end of the spring shaft (873) is fixedly connected with an inner wall of one side of the travel groove (86), one end of the clamping rod (875) is clamped into a clamping hole provided on the bottom surface of the slider (872), and the top end of the pressing rod (874) passes through and extends to the outer side of the placement table (85).
9. The microorganism detection apparatus according to claim 8, wherein an oil storage component (6) is provided on the inner wall of the bottom surface of the casing (4), the oil storage component (6) is used for storing and guiding out lubricating oil, the oil storage component (6) is located between the two polishing components (7), the oil storage component (6) comprises an oil box (62), an oil bag is provided inside the oil box (62), an oil pipe is fixedly installed on the side wall of the oil bag, one end of the oil pipe penetrates and extends to the outer side of the oil box (62), an oil injection head (61) is fixedly installed at one end of the oil pipe, and one end of the oil injection head (61) is in close contact with the transmission bevel gear (53).
10. The detection method of a microorganism detection apparatus according to any one of claims 1 to 9, comprising the steps of:
s1, when a sample needs to be placed and positioned, the sample is directly placed in the center of a detection sample placement mechanism (8), at the moment, a pressing rod (874) is pressed to descend, a clamping rod (875) descends synchronously, the top end of the clamping rod (875) is separated from a clamping hole at the bottom of a sliding block (872), at the moment, a spring shaft (873) bounces the side of an arc-shaped positioning plate (871), the other arc-shaped positioning plate (871) is triggered synchronously, and the two arc-shaped positioning plates (871) simultaneously position the sample;
s2, when the microorganism detection is carried out, a detection sample added with a detection reagent is placed on a detection sample placement mechanism (8), during the detection, the observation mirror (3) can finely observe the detection sample, and an observed image can be displayed in a picture of the detector (2) in real time;
s3, if the sample microorganisms need to be zoomed in and carefully observed, an adjusting motor (52) of an adjusting assembly (5) is started, the adjusting motor (52) can control a two-way stud (51) to rotate, a transmission bevel gear (53) can synchronously rotate, the transmission bevel gear (53) drives an adjusting stud (82) with a driven bevel gear (81) to rotate through a meshing effect, the adjusting stud (82) can control a placing table (85) to be lifted, at the moment, the distance between the sample placed on the placing table (85) and an observation mirror (3) is zoomed in, close-range observation can be carried out, and at the same time, when the two-way stud (51) rotates, two moving seats (73) on the two-way stud (51) can be driven to be mutually close, at the moment, two arc-shaped light plates (71) are gradually close to the sample, so that more effective light supplementing can be given to the sample, and the fine observation effect on the microorganisms in the sample can be further promoted;
s4, if regional observation is carried out relative to microorganisms in the sample, the adjusting stud (82) is controlled to rotate reversely, the automatic falling height of the sample placing mechanism (8) is detected, and the two arc-shaped light plates (71) are far away, so that the sample is subjected to more divergent illumination, and the sample is widely and comprehensively observed;
s5, when adjusting the position of the polishing component (7) and detecting the sample placement mechanism (8), the pressing piece (74) can synchronously move sideways, at this moment, the pressing piece (74) can extrude oil bags in the oil storage component (6), oil in the extruded oil bags can be led into the driven bevel gear (81) through the oil injection head (61), oil on the driven bevel gear (81) can be transmitted to the transmission bevel gear (53) through meshing transmission, effective lubrication of the driven bevel gear (81) and the transmission bevel gear (53) is achieved, friction between the driven bevel gear and the transmission bevel gear (53) is reduced, and transmission efficiency and smoothness of the driven bevel gear (81) and the transmission bevel gear (53) are improved.
CN202310261159.8A 2023-03-14 2023-03-14 Microorganism detection device and detection method thereof Active CN116333872B (en)

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US20150022883A1 (en) * 2013-07-18 2015-01-22 University Of Maine System System and apparatus for illuminating a specimen
KR20190036584A (en) * 2017-09-28 2019-04-05 주식회사 지디텍 A Optical Apparatus
CN110794126A (en) * 2019-11-04 2020-02-14 苏州煜水生物科技有限公司 Biological sample detecting instrument convenient to adjust
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CN112630219A (en) * 2020-12-14 2021-04-09 张小辉 Medical image viewing device convenient for positioning disease source position
CN214249380U (en) * 2021-03-24 2021-09-21 廖惜玉 Miniature illuminating lamp for manufacturing glasses
CN217521436U (en) * 2022-06-07 2022-09-30 广检检测技术(上海)有限公司 Automatic polarizing device of polarizing microscope

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103502422A (en) * 2011-03-04 2014-01-08 Lbt创新有限公司 Image capture and lighting apparatus
US20150022883A1 (en) * 2013-07-18 2015-01-22 University Of Maine System System and apparatus for illuminating a specimen
KR20190036584A (en) * 2017-09-28 2019-04-05 주식회사 지디텍 A Optical Apparatus
KR20200132417A (en) * 2019-05-17 2020-11-25 주식회사 제이엔옵틱 Apparatus for holding sample and method for holding sample
CN110794126A (en) * 2019-11-04 2020-02-14 苏州煜水生物科技有限公司 Biological sample detecting instrument convenient to adjust
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