CN116331664B - Kit and method for detecting breast cancer tumor markers - Google Patents
Kit and method for detecting breast cancer tumor markers Download PDFInfo
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- CN116331664B CN116331664B CN202310096962.0A CN202310096962A CN116331664B CN 116331664 B CN116331664 B CN 116331664B CN 202310096962 A CN202310096962 A CN 202310096962A CN 116331664 B CN116331664 B CN 116331664B
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- fixedly connected
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- breast cancer
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- 206010006187 Breast cancer Diseases 0.000 title claims abstract description 19
- 208000026310 Breast neoplasm Diseases 0.000 title claims abstract description 19
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title abstract description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 27
- 235000011089 carbon dioxide Nutrition 0.000 claims abstract description 27
- 230000007306 turnover Effects 0.000 claims abstract description 24
- 238000005452 bending Methods 0.000 claims description 23
- 239000012153 distilled water Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 238000001514 detection method Methods 0.000 claims description 14
- 239000000439 tumor marker Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 6
- 230000001681 protective effect Effects 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000011534 wash buffer Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 29
- 238000002965 ELISA Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 102000016267 Leptin Human genes 0.000 description 3
- NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 description 3
- 229940039781 leptin Drugs 0.000 description 3
- 102000011690 Adiponectin Human genes 0.000 description 2
- 108010076365 Adiponectin Proteins 0.000 description 2
- 108010092277 Leptin Proteins 0.000 description 2
- 230000000711 cancerogenic effect Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 101000775469 Homo sapiens Adiponectin Proteins 0.000 description 1
- 101001063991 Homo sapiens Leptin Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 102000057799 human ADIPOQ Human genes 0.000 description 1
- 210000004293 human mammary gland Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D43/00—Lids or covers for rigid or semi-rigid containers
- B65D43/14—Non-removable lids or covers
- B65D43/16—Non-removable lids or covers hinged for upward or downward movement
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D51/00—Closures not otherwise provided for
- B65D51/24—Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D55/00—Accessories for container closures not otherwise provided for
- B65D55/02—Locking devices; Means for discouraging or indicating unauthorised opening or removal of closure
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57415—Specifically defined cancers of breast
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Mechanical Engineering (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
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- Oncology (AREA)
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Abstract
The invention provides a kit and a method for detecting breast cancer tumor markers, and relates to the technical field of breast cancer, the kit comprises a bent plate, wherein the front end of the bent plate is fixedly connected with a thread seat A, the top of the bent plate is fixedly connected with a rubber enclosing strip, the top horizontal plane of the bent plate is fixedly connected with a magnetic strip A, the top of the rear end of the bent plate is provided with a turnover cover in a rotating connection manner, the top of the front end elevation of the turnover cover is fixedly connected with a limiting sleeve, the inside of the limiting sleeve horizontally penetrates through a circular through hole, the turnover cover is upwards turned by holding a top frame, a side plate can be separated from a gap between a storage groove and a dry ice sleeve, a torsional spring C can drive a vertical shaft to reset and rotate, the vertical shaft can drive the side plate to deflect ninety degrees, a groove bracket can support the bottom edge of the turnover cover, the side plates on the left side and the right side can be outwards unfolded, and the left side and the right side of a reagent can be unfolded side by side, and the problem that the reagent is inconvenient to be unfolded side by side after the existing kit is opened, and the reagent is inconvenient to take rapidly is solved.
Description
Technical Field
The invention relates to the technical field of breast cancer, in particular to a kit and a method for detecting a breast cancer tumor marker.
Background
Tumor markers are chemical and biological substances reflecting the existence of tumors, are only found in embryo tissues when the tumor markers are not present in normal adult tissues, or are in a content in the tumor tissues which is greatly higher than that in normal tissues, and the existence or the quantity of the tumor markers can indicate the property of the tumor, so that the breast cancer can be checked by using a reagent to detect the content of the blood markers of a user.
The inventors team used human adiponectin and leptin assay kits (e.g., us eBioscience) in a scholarly article ("preliminary study of serum adiponectin, leptin levels and breast cancer correlation," Yu Zhigang et al, "university of shandong journal (medical edition), volume 50, phase 6) to determine adiponectin levels and leptin levels in all specimens by enzyme-linked immunosorbent assay (ELISA). Furthermore, the invention patent CN112646888A discloses a detection kit of methylation biomarker related to breast cancer.
However, the above-mentioned kit is inconvenient to carry and transport for a long time, and the user is inconvenient to recycle the kit, and the kit is inconvenient to open and then spread the reagents side by side, so that the reagents are convenient to take rapidly, and the user is inconvenient to directly operate, so that the above problems need to be solved.
Disclosure of Invention
In view of this, the invention provides a kit and a method for detecting breast cancer tumor markers, which can drive a primary platform to vertically translate by rotating an adjusting screw B, can adjust the height of an expansion card, can drive a reciprocating screw C to vertically rotate by rotating a worm hand wheel A, can vertically and horizontally adjust the expansion card, and is convenient for adjusting and adapting PCI expansion slots and memory sockets which are pulled and plugged at different angles.
The invention provides a purpose and an efficacy of a kit for detecting breast cancer tumor markers, which specifically comprises a bending plate; the front end of the bending plate is fixedly connected with a thread seat A, the top of the bending plate is fixedly connected with a rubber surrounding strip, and the horizontal plane of the top of the bending plate is fixedly connected with a magnetic stripe A; the top of the rear end of the bent plate is provided with a turnover cover through rotating connection, the top of the vertical face of the front end of the turnover cover is fixedly connected with a limit sleeve, a circular through hole is horizontally penetrated in the limit sleeve, the front end of the limit sleeve is fixedly connected with a tension spring, the inner side of the tension spring is fixedly connected with an inserting rod, the inserting rod is slidably connected to the inner side of the circular through hole of the limit sleeve, the vertical face of the front end of the turnover cover is fixedly connected with a display screen, the rear end of the display screen is fixedly connected with a temperature sensor, the temperature sensor is positioned on the inner side of the turnover cover, the bottom of the vertical face of the front end of the turnover cover is fixedly connected with a thread seat B, the inner side of the thread seat B is provided with a fastening screw through threaded connection, and the bottom of the turnover cover and the rear side are provided with surrounding grooves; the top of the turnover cover is provided with a top frame through rotary connection; the bottom of the front end of the top frame is provided with an inclined plane, and a limiting hole horizontally penetrates through the inside of the inclined plane.
Optionally, the bottom of the rear end of the top frame is fixedly connected with a bottom frame, the left side and the right side of the inside of the turnover cover are fixedly connected with dry ice sleeves, and the number of the dry ice sleeves is two.
Optionally, a fixing piece A is fixedly connected to the vertical elevation on the inner side of the top of the dry ice sleeve, and a square groove vertically penetrates through the inside of the fixing piece A.
Optionally, the top of the dry ice sleeve is provided with a flip through rotating connection, and the edge of the inner side of the flip is fixedly connected with a fixing sheet B.
Optionally, the inside pull rod that is connected with through sliding in stationary blade B, the pull rod outside fixedly connected with torsional spring A, torsional spring A bottom and stationary blade B top fixed connection, pull rod bottom fixedly connected with stopper.
Optionally, the inside fixedly connected with spread groove of flip lid, the spread groove is located two sets of dry ice cover insides, and the spread groove inboard is provided with the distilled water tank.
Optionally, the top of the distilled water tank is fixedly connected with a top bridge, an inner groove is formed in the vertical face of the rear end of the distilled water tank, and a sealing cover is arranged on the inner side of the inner groove through threaded connection.
Optionally, the bending plate top is provided with the inner cup through rotating the connection, and inner cup front side bottom fixedly connected with magnetic stripe B, the inner cup inboard is provided with micropore ELISA plate.
Optionally, vertical shafts are arranged at the vertical surface of the rear side of the bending plate in a rotating connection manner, the number of the vertical shafts is two, a torsion spring B is arranged at the joint of the top of the vertical shafts and the bending plate, a side plate is fixedly connected to the curved side surface of the vertical shafts, a groove bracket is fixedly connected to the top of the side plate, a display rack is fixedly connected to the front side of the side plate, a circular through hole vertically penetrates through the inside of the display rack, a supporting plate is fixedly connected to the front side of the display rack, and washing buffer solution, substrate, stop solution, detection antibody-HRP and a sample diluent bottle are penetrated on the inner side of the display rack.
Optionally, the exhibition frame top is provided with the cross axle through rotating the connection, and cross axle quantity sets up into four groups, and the cross axle is inside to be provided with the frame through coaxial coupling, and the cross axle end is provided with the bevel gear through coaxial coupling, and bevel gear meshing transmission, cross axle and exhibition frame articulated department are provided with torsional spring C, and cross axle curved surface fixedly connected with presses the button, and the cross axle outside is provided with the protective sheath, exhibition frame front side fixedly connected with ladder frame, ladder frame front end fixedly connected with nameplate, six pipe standard substance are worn to be equipped with in the ladder frame inboard.
The detection method of the breast cancer tumor marker is completed by using the kit.
Advantageous effects
Through placing dry ice into inside the dry ice cover, through flip with its lock back, make its stopper of bottom make its with the square groove align the back through turning over the pull rod, can make the stopper insert stationary blade A bottom downwards, reset through torsional spring A and rotate, can make it drive the stopper and rotate, can make stopper top and square groove spacing, can conveniently close flip, can make dry ice cool down reagent in the box after taking out the kit, be convenient for transport reagent.
In addition, the user can break away from spacing hole through pulling the inserted bar, can make the roof-rack upwards overturn, can make the chassis remove with the support spacing at distilled water tank top, the user can take out the holding vessel with the distilled water tank, the user can pack distilled water to the distilled water tank, the be convenient for carry out repeatedly used to the kit.
In addition, after the user rotates the fastening screw to enable the fastening screw to be separated from the thread seat A, the turnover cover and the bending plate can be opened, the user can hold the top frame to turn the turnover cover upwards, the side plates can be separated from the gap between the storage groove and the dry ice sleeve, the torsional spring C can drive the vertical shaft to reset and rotate, the vertical shaft can drive the side plates to deflect ninety degrees, the groove support can support the bottom edge of the turnover cover, the side plates on the left side and the right side can be unfolded outwards, the reagents can be unfolded side by side on the left side and the right side, and the reagent can be unfolded and taken conveniently.
In addition, after the user turns over the flip lid and opens, the user can be through supporting flip lid and curb plate, can make it shelter from the operation area, can prevent outside dust impurity and mix into reagent.
Drawings
In order to more clearly illustrate the technical solution of the embodiments of the present invention, the drawings of the embodiments will be briefly described below.
The drawings described below are only for illustration of some embodiments of the invention and are not intended to limit the invention.
In the drawings:
FIG. 1 is a schematic top view of the whole of a kit according to an embodiment of the present invention.
Fig. 2 is an enlarged partial schematic view of the invention of fig. 1 a.
Fig. 3 is a schematic perspective exploded view of a turnover cover of a kit according to an embodiment of the present invention.
Fig. 4 is an enlarged partial schematic view of B of fig. 3 in accordance with the present invention.
Fig. 5 is a schematic bottom perspective view of a flip top of a kit according to an embodiment of the invention.
Fig. 6 is an enlarged partial schematic view of C of fig. 5 in accordance with the present invention.
FIG. 7 is a schematic view showing the overall front side three-dimensional structure of a kit according to an embodiment of the present invention.
Fig. 8 is a schematic perspective exploded view of a display rack of a kit according to an embodiment of the present invention.
List of reference numerals
1. A bending plate;
101. a thread seat A; 102. a rubber surrounding strip; 103. a magnetic stripe A;
2. a flip cover;
201. a limit sleeve; 202. a tension spring; 203. a rod; 204. a display screen; 205. a thread seat B; 206. fastening a screw; 207. a surrounding groove;
3. a top frame;
301. an inclined plane; 302. a limiting hole; 303. a chassis;
4. a dry ice sleeve;
401. a fixing piece A; 402. a square groove; 403. a flip cover; 404. a fixing piece B; 4041. a pull rod; 4042. a torsion spring A; 405. a limiting block;
5. a storage tank;
501. a distilled water tank; 502. a top bridge; 503. an inner groove;
6. an inner cover;
601. a magnetic stripe B; 602. a microporous ELISA plate;
7. a vertical shaft;
701. a torsion spring B; 702. a side plate; 7021. a groove bracket; 703. a display rack; 704. a supporting plate; 705. a horizontal axis; 706. building a frame; 707. bevel gears; 708. a torsion spring C; 709. pressing a button; 710. a protective sleeve;
8. a step frame;
801. a nameplate.
Description of the embodiments
Embodiments of the present invention are described in further detail below with reference to the accompanying drawings and examples.
Examples: please refer to fig. 1 to 8:
the invention provides a kit for detecting breast cancer tumor markers, which comprises a bent plate 1; the front end of the bending plate 1 is fixedly connected with a thread seat A101, the top of the bending plate 1 is fixedly connected with a rubber enclosing strip 102, and the horizontal plane of the top of the bending plate 1 is fixedly connected with a magnetic stripe A103; the top of the rear end of the bent plate 1 is provided with a bent plate 2 through rotary connection, the top of the front end elevation of the bent plate 2 is fixedly connected with a limit sleeve 201, a circular through hole is horizontally penetrated in the limit sleeve 201, the front end of the limit sleeve 201 is fixedly connected with a tension spring 202, the inner side of the tension spring 202 is fixedly connected with an inserting rod 203, the inserting rod 203 is slidably connected to the inner side of the circular through hole of the limit sleeve 201, the front end elevation of the bent plate 2 is fixedly connected with a display screen 204, the rear end of the display screen 204 is fixedly connected with a temperature sensor, the temperature sensor is positioned on the inner side of the bent plate 2, the bottom of the front end elevation of the bent plate 2 is fixedly connected with a thread seat B205, the inner side of the thread seat B205 is provided with a fastening screw 206 through threaded connection, and a surrounding groove 207 is formed between the bottom and the rear side of the bent plate 2; the top of the turnover cover 2 is provided with a top frame 3 through rotary connection; the inclined plane 301 has been seted up to the front end bottom of roof-rack 3, the inside level of inclined plane 301 runs through there is spacing hole 302, bent plate 1 top is provided with inner cup 6 through rotating the connection, inner cup 6 front side bottom fixedly connected with magnetic stripe B601, inner cup 6 inboard is provided with micropore ELISA plate 602, roof-rack 3 rear end bottom fixedly connected with chassis 303, the inside left and right sides fixedly connected with dry ice cover 4 of bent plate 2, dry ice cover 4 quantity sets up to two sets of, the inside perpendicular facade department fixedly connected with stationary blade A401 of dry ice cover 4 top, the inside square groove 402 that runs through perpendicularly of stationary blade A401, dry ice cover 4 top is provided with flip 403 through rotating the connection, flip 403 inboard edge fixedly connected with stationary blade B404, stationary blade B404 inside is provided with pull rod 4041 through sliding connection, the outside fixedly connected with torsional spring A4042 of pull rod 4041, torsional spring A4042 bottom and stationary blade B404 top fixedly connected with stopper 405, through placing dry ice cover 4 inside back into behind the flip 403, make its bottom stopper 405 make it align with square groove through flip 403 through rotating pull rod 4041, can make its bottom stopper 405 and square groove to rotate the inside the flip 405, can carry out the reagent box with the flip 405 through rotating the flip 405, can carry out the reagent box with the inside the flip 405, can be convenient for carrying the reagent box is cooled down, can be cooled down by the reagent box is convenient, the reagent box is cooled down, can be cooled down by the top is convenient to take out the reagent box is convenient to carry the top and can be cooled down and the top is convenient to carry down.
As shown in fig. 3 to 8, the inner side of the turnover cover 2 is fixedly connected with a storage tank 5, the storage tank 5 is located on the inner sides of two groups of dry ice sleeves 4, a distilled water tank 501 is arranged on the inner side of the storage tank 5, a top bridge 502 is fixedly connected to the top of the distilled water tank 501, an inner groove 503 is formed in the vertical face of the rear end of the distilled water tank 501, a sealing cover is arranged on the inner side of the inner groove 503 through threaded connection, a user can pull the inserting rod 203 to enable the inserting rod 203 to be separated from the limiting hole 302, the top frame 3 can be turned upwards, the bottom frame 303 can be separated from supporting limiting of the top of the distilled water tank 501, the user can take out the distilled water tank 501 from the storage tank 5, and the user can fill the distilled water into the distilled water tank 501, so that the kit can be reused conveniently.
As shown in fig. 2 to 8, vertical shafts 7 are arranged at the vertical surface at the rear side of the bending plate 1 through rotation connection, the number of the vertical shafts 7 is two, torsion springs B701 are arranged at the hinging position of the tops of the vertical shafts 7 and the bending plate 1, side plates 702 are fixedly connected to the curved sides of the vertical shafts 7, groove brackets 7021 are fixedly connected to the tops of the side plates 702, display frames 703 are fixedly connected to the front sides of the side plates 702, circular through holes vertically penetrate through the inside of the display frames 703, supporting plates 704 are fixedly connected to the front sides of the display frames 703, washing buffer solution, substrates, terminating solution, detection antibody-HRP and sample diluent bottles are penetrated through the inner sides of the display frames 703, transverse shafts 705 are arranged at the tops of the display frames 703 through rotation connection, the number of the transverse shafts 705 is four, a frame 706 is arranged at the inner sides of the transverse shafts 705 through coaxial connection, bevel gears 707 are arranged at the tail ends of the transverse shafts 705 through coaxial connection, engaged transmission of the bevel gears 707 are meshed, torsion springs C708 are arranged at the hinging positions of the transverse shafts 705 and the display frames 703, the curved side of the transverse shaft 705 is fixedly connected with a press button 709, the outer side of the transverse shaft 705 is provided with a protective sleeve 710, the front side of the display frame 703 is fixedly connected with a ladder frame 8, the front end of the ladder frame 8 is fixedly connected with a nameplate 801, six standard products are penetrated and arranged on the inner side of the ladder frame 8, after a user rotates the fastening screw 206 to separate from the screw seat A101, the folding cover 2 and the bending plate 1 can be opened, the user can hold the top frame 3 to upwards turn the folding cover 2, the side plate 702 can be separated from the gap between the storage tank 5 and the dry ice sleeve 4, the torsion spring C708 can drive the vertical shaft 7 to reset and rotate, the vertical shaft 7 can drive the side plate 702 to deflect ninety degrees, the groove bracket 7021 can support the bottom edge of the folding cover 2, the side plates 702 on the left side and the right side can be outwards spread the reagent side by side, after the user turns the inner cover 6 upwards, the microporous ELISA plate 602 can be taken out, the detection operation is conveniently performed by using the high-precision sample injector, a user can press the press button 709 to drive the transverse shaft 705 to rotate, bevel gears 707 on the left side and the right side of the transverse shaft 705 can drive, four groups of transverse shafts 705 can synchronously rotate, four groups of shelves 706 can be driven to overturn upwards, the user is convenient to take reagent bottles on the inner side of the display frame 703, and after the reagent bottles are reloaded, the transverse shaft 705 is driven to reset and rotate by a torsion spring C708, so that the shelves 706 limit the reagent bottles.
The detection method of the breast cancer tumor marker is completed by using the kit, and specifically comprises the following steps:
after the dry ice is placed into the dry ice sleeve 4 and buckled by turning the flip cover 403, the limiting block 405 at the bottom of the pull rod 4041 is aligned with the square groove 402, the limiting block 405 can be downwards inserted into the bottom of the fixing piece A401, the limiting block 405 can be driven to rotate by the reset rotation of the torsion spring A4042, the top of the limiting block 405 and the square groove 402 can be limited, the flip cover 403 can be conveniently closed, the reagent in the kit can be cooled by the dry ice after the kit is taken out, the reagent is convenient to transport and carry, the user can pull the inserting rod 203, the inserting rod 203 can be separated from the limiting hole 302, the top frame 3 can be upwards turned, the supporting limit of the bottom frame 303 and the top of the distilled water tank 501 can be released, the user can take the distilled water tank 501 out of the storage groove 5, the distilled water can be filled into the distilled water tank 501, the reagent box can be reused conveniently, after a user rotates the fastening screw 206 to separate from the thread seat A101, the turnover cover 2 and the bending plate 1 can be opened, the user can hold the top frame 3 to turn the turnover cover 2 upwards, the side plate 702 can separate from the gap between the storage groove 5 and the dry ice sleeve 4, the torsion spring C708 can drive the vertical shaft 7 to reset and rotate, the vertical shaft 7 can drive the side plate 702 to deflect ninety degrees, the groove bracket 7021 can support the bottom edge of the turnover cover 2, the side plates 702 on the left side and the right side can be unfolded outwards, the reagent can be unfolded side by side on the left side and the right side, the user can take out the microporous enzyme-labeled plate 602 after the inner cover 6 is turned upwards, the detection operation is convenient to use a high-precision sample applicator, the user can press the press button 709 to drive the transverse shaft 705 to rotate, the bevel gears on the left side and the right side of the transverse shaft 705 can drive the bevel gears on the left side and the right side of the transverse shaft 707, the four groups of transverse shafts 705 can be synchronously rotated, the four groups of transverse shafts 706 can be driven to upwards overturn, a user can conveniently take reagent bottles on the inner sides of the display frames 703, after the reagent bottles are reloaded, the transverse shafts 705 are driven to reset and rotate through the torsion springs C708, the reagent bottles can be limited by the lifting frames 706, the reagent boxes adopt a double antibody one-step sandwich method enzyme-linked immunosorbent assay (ELISA), a sample, a standard substance and an HRP marked detection antibody are sequentially added into coated micropores of a pre-coated human breast cancerogenic substance (BC) capture antibody, after incubation and thorough washing, the color development is carried out by using a substrate TMB, the TMB is converted into blue under the catalysis of peroxidase, and is converted into final yellow under the action of acid. The color shade and human mammary gland carcinogen (BC) in the sample are positively correlated, and the absorbance (OD value) is measured at the wavelength of 450nm by using an enzyme-labeled instrument, so as to calculate the concentration of the sample.
Claims (5)
1. A kit for detection of breast cancer tumor markers, comprising: a bending plate (1); the front end of the bending plate (1) is fixedly connected with a thread seat A (101), the top of the bending plate (1) is fixedly connected with a rubber surrounding strip (102), and the horizontal plane of the top of the bending plate (1) is fixedly connected with a magnetic stripe A (103); the novel folding plate comprises a folding cover (2) arranged at the top of the rear end of the folding plate (1) in a rotating connection manner, a limit sleeve (201) is fixedly connected to the top of the front end elevation of the folding cover (2), a circular through hole is horizontally penetrated inside the limit sleeve (201), a tension spring (202) is fixedly connected to the front end of the limit sleeve (201), an inserting rod (203) is fixedly connected to the inner side of the tension spring (202), the inserting rod (203) is slidably connected to the inner side of the circular through hole of the limit sleeve (201), a display screen (204) is fixedly connected to the front end elevation of the folding cover (2), a temperature sensor is fixedly connected to the rear end of the display screen (204), the temperature sensor is located on the inner side of the folding cover (2), a threaded seat B (205) is fixedly connected to the bottom of the front end elevation of the folding cover (2), a fastening screw (206) is arranged on the inner side of the threaded seat B (205) in a threaded connection manner, and a surrounding groove (207) is formed in the bottom and the rear side of the folding cover (2). The top of the turnover cover (2) is provided with a top frame (3) through rotary connection; an inclined plane (301) is formed at the bottom of the front end of the top frame (3), and a limiting hole (302) is horizontally penetrated in the inclined plane (301); the bottom of the rear end of the top frame (3) is fixedly connected with a bottom frame (303), the left side and the right side of the inside of the turnover cover (2) are fixedly connected with dry ice sleeves (4), and the number of the dry ice sleeves (4) is two; the inner side of the turnover cover (2) is fixedly connected with a storage tank (5), the storage tank (5) is positioned at the inner sides of the two groups of dry ice sleeves (4), and a distilled water tank (501) is arranged at the inner side of the storage tank (5); an inner cover (6) is arranged at the top of the bending plate (1) through rotary connection, a magnetic strip B (601) is fixedly connected to the bottom end of the front side of the inner cover (6), and a microporous enzyme label plate (602) is arranged on the inner side of the inner cover (6); the vertical surface at the rear side of the bending plate (1) is provided with vertical shafts (7) through rotating connection, the number of the vertical shafts (7) is two, the hinged position of the top of the vertical shafts (7) and the bending plate (1) is provided with torsion springs B (701), the curved side surface of the vertical shafts (7) is fixedly connected with a side plate (702), the top of the side plate (702) is fixedly connected with a groove bracket (7021), the front side of the side plate (702) is fixedly connected with a display rack (703), a circular through hole vertically penetrates through the inside of the display rack (703), the front side of the display rack (703) is fixedly connected with a supporting plate (704), and washing buffer solution, substrate, termination solution, detection antibody-HRP and a sample diluent bottle are penetrated inside the display rack (703); the top of the display rack (703) is provided with cross shafts (705) through rotary connection, the number of the cross shafts (705) is four groups, the inner part of the cross shafts (705) is provided with a rack (706) through coaxial connection, the tail end of the cross shaft (705) is provided with a bevel gear (707) through coaxial connection, the bevel gear (707) is meshed for transmission, the hinge joint of the transverse shaft (705) and the display rack (703) is provided with a torsion spring C (708), the curved side surface of the transverse shaft (705) is fixedly connected with a press button (709), the outer side of the transverse shaft (705) is provided with a protective sleeve (710), the front side of the display rack (703) is fixedly connected with a ladder rack (8), the front end of the ladder rack (8) is fixedly connected with a nameplate (801), and six-tube standard products are arranged on the inner side of the ladder rack (8) in a penetrating mode.
2. A kit for breast cancer tumor marker detection as claimed in claim 1, wherein: a fixing piece A (401) is fixedly connected to the vertical face on the inner side of the top of the dry ice sleeve (4), and a square groove (402) vertically penetrates through the fixing piece A (401).
3. A kit for breast cancer tumor marker detection as claimed in claim 1, wherein: the top of the dry ice sleeve (4) is provided with a flip (403) through rotating connection, and a fixing sheet B (404) is fixedly connected to the edge of the inner side of the flip (403).
4. A kit for breast cancer tumor marker detection as claimed in claim 3, wherein: the fixing piece B (404) is internally provided with a pull rod (4041) in a sliding way, the outer side of the pull rod (4041) is fixedly connected with a torsion spring A (4042), the bottom of the torsion spring A (4042) is fixedly connected with the top of the fixing piece B (404), and the bottom of the pull rod (4041) is fixedly connected with a limiting block (405).
5. A kit for breast cancer tumor marker detection as claimed in claim 1, wherein: the top of the distilled water tank (501) is fixedly connected with a top bridge (502), an inner groove (503) is formed in the vertical face of the rear end of the distilled water tank (501), and a sealing cover is arranged on the inner side of the inner groove (503) through threaded connection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310096962.0A CN116331664B (en) | 2023-02-10 | 2023-02-10 | Kit and method for detecting breast cancer tumor markers |
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| CN202310096962.0A CN116331664B (en) | 2023-02-10 | 2023-02-10 | Kit and method for detecting breast cancer tumor markers |
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| CN116331664A (en) | 2023-06-27 |
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