CN1163046A - Method for attracting crustacean and compositions including feed enhanced materials - Google Patents

Abstract

One kind of method of attacting crustacean from the habital water area the required place and one kind of intensified feed composite for crustacean. The said composite includes basic feed component and feed intensifying material with the density and the amount producing attraction and/ or stimulation and/or excitation.

Description

A kind of composition that attracts the method for shellfish and contain the feed enhancement material

Put down in writing the trial that stimulates different aquatic products several times with feed on the document, and be applicable in creation and also obtain high efficiency aspect the equipment of definite process of stimulant that aquatic biological is good and attractant.So far, United States Patent (USP) 4,250, No. 835 and 4,249,480 revealed the equipment and the method for culture shrimp.The larval phase of shrimp, is controlled but provide general enveloping field formula to culture equipment simultaneously, does not control male and female and becomes shrimp freely to come in and go out, with the mating that keeps most of shrimps, stablizing of laying eggs, hatch.This operating system has been revealed provides the filtration equipment that filters culture fluid and the collection equipment during from enveloping field culture fluid inflow filter in the previously selected time.United States Patent (USP) the 4th, 828 has been revealed a kind of fish attractant of vision No. 829, and it helps to scatter the fragrance that tradition uses and the attractant of taste.The composition of this fish attractant comprises one or more oil, for example mineral oil, cod oil, pilchardine, herring oil, too anise oil, salmon oil and pigment, spices, fishlike smell, swim pigment and reflective particle, the attractant that both can be used as vision also helps to control dispersing of oil and fragrance.

Lombardo and colleague thereof were published in Comp.Biochem.Physi-ol in 1992, Vol.101C.No.2, one piece of paper on the 389-398 page or leaf " fresh water snail (Planorbarius corneus (L)) with amino acid and derivative thereof as the signal of seeking food ", once revealed that this fresh water snail to different seed amino acids, was different reactions as l-winter propylhomoserin, d-alanine, histamine, proline and aspartic acid.

It must be noted that compound has following structural formula:

X representative a kind of " monovalent anion " as chlorion or smelly ion, can be lured vertebrate fish, as snapper and carp herein.This is published in Japanese patent application J91/27231 number (Nakajima), takes passages in Chem.Abstract, on the Vol.115:113303n.

Japanese patent application J07/050998 number, take passages " the Derwent chemical patent index wall bulletin type digest " published on May 26th, 1 on 95 year the 17th phase.

Its summary main points are as follows:

YAES?D13?95-127296/17 *JP07050998-A

Raise the growth promoter that fish are used. comprise D-aminoglucose or its esters

YAUSU?SUISAN?KAGAKU?KOGYO?KK?93.08.09?JP-217092BC05C03(95.02.28)A23K1/16，1/18

Medicament comprises at least a D-aminoglucose and its esters.

The benefit of using-effectively promote growth, cost is low, improves feeding efficiency.

(4pp)Dwg.No.0/0)

C95-058168

The invention provides a kind ofly lure, excited, stimulate and/or excite at least a crustacean method.How to comprise with above-mentioned shell-fish from its waters of perching or the water surface introduce in certain location or the volume.This molten long-pending include lure, excited, the saline solution that stimulates and/or excite the shell-fish effect.Any material (being referred to as " ASIE " material) below comprising at least in its solution:

(I) N-acetyl group-D-aminoglucose is the mixture with two kinds of epimers of following array structure:

With

(II) have a kind of S-methylmethionine halogenation sulfonium at least, its structural formula is as follows:

(wherein X is cl anion or bromine anions) be methionine (III), and its structural formula is as follows:

(IV) hydrate of trimethyl amine oxide, its structural formula is as follows: (V) (R) (S)-octene-3-alcohol, its structural formula is as follows: Be a kind of mixture of isomer, its structural formula is as follows:

(VI) 3-(methylthio) propanal, its structural formula is as follows:

(VII) methyl-sulfoxide, its structural formula is as follows:

(VII) 50: 50 mole: the scatol of molar ratio/indoles mixture, its structure

Formula is as follows:

Scatol: indoles:

(IX) S-methyl first sulphur propionic acid halogenation sulfonium, its structural formula is as follows:

(wherein, X is cl anion or bromine anions) be ammonium chloride (XI) ammonium acetate (XII) acetic acid (X), and its structural formula is as follows:

(XIII) glucose (XIV) raw sugar (containing sucrose and impurity) is such as, the Osceo-that is produced by the Osceola farm

La brown sugar, this farm is positioned at Pahokee, Florida.(XV) (have another name called TALIN, be Britain Tate﹠amp to Thaumatin; Lyle Co., Ltd

Registration mark) is Thaumatin B and Thaumatin I, Thaumatin

The mixture of II.Thaumatin I is also with following symbolic representation:

〔Lys ⁴⁵，Asp ¹¹³，Asp ¹³⁷〕

In detail special narration can be referring to US Patent specification the 5th, 221, No. 624; (XVI) 2-methyl-3-(methyl two sulphur) furans has another name called (MOS) or " Methy-

Loxycyclosulfide ^TM" be the registration mark of international fragrance essence corporation, its knot

The structure formula is as follows: (XVII) ammoniacal liquor (XVIII) yeast extract (XIX) D-aminoglucose is a kind of mixture of epimer, and its structural formula is as follows:

(XX) 2-methylfuran mercaptan (has another name called and is OXYCYCLOTHIONE-03O ^TM

Being the registration mark of international fragrance essence corporation) its structural formula is as follows:

Use separately or with analeptic, stimulant, attractant and/or the exciting agent of natural existence, as the extract of natural shell-fish/siphonopods, combined and be applied near aforementioned suitable place or the volume shell-fish.How the present invention narrates again in perching the volume scope in waters, the process of impelling the shell-fish excitement, lure and/or exciting.How to comprise by one of above-mentioned material (i.e. " ASIE " article of appointment) make contain excitement, lure, excite and/or irritaiting concentration be applied near the aforementioned crustacean aqueous solution of perching the volume.

Shell-fish as an example has, and shrimp belongs to the kind of Penaeus genus, as Penaeussetiferus, and Penaeus vannamei and Penaeus aztecus and cray Procam-brus clarkii.

Determine to the shell-fish member lure, stimulate, excite and/or the final goal of excitation is, strengthen raising course (for example, the quantity and the speed of food picked-up and metabolism)

Therefore, the present invention also relates to raise crustacean feed ingredient, comprise: (i) feed ingredient in the prior art is (as RANGEN-35, be to be protein 35%, lipase 37 %, fish meal, fish oil, and the mixture of a spot of mineral matter and vitamin), also comprise, as L-ascorbic acid-2-phosphate and its esters, the J94/093 that delivers as Japan, 821 and J94/093,822, belong to Showa Denko KK company, and taken passages " the Derwent index of patents wall bulletin type digest " published on January 4th, 1 on 94 year the 45th phase, it thes contents are as follows: the feed addictive of SHOW D-13 87-253800/36=JP94093821-B2 fish culture use comprises L-ascorbic acid-2-phosphoric acid and its esters, as activating agent

SHOWA?DENKO?KK?86.01.30 86JP-016739

C01(94.11.24)JP62175142-A?A23K1/18.1/16.A61K?31/375//C07D?307/62

According to JP62175142A

Claimed containing as the L-ascorbic acid-2-phosphoric acid of activating agent and the feed addictive of its esters.Purposes/effect: as a kind of stable food additives, to the fish tool ascorbic acid effect of culturing.

Give one example, a slice feedstuff sheet (basic feed) contains the vitamin that 55% white bait powder, 35% L-potato starch, 5% soya-bean oil, 3%Mc Collum and 5% contain ascorbic acid.This is used to lack ascorbic acid and raises the district.When being used in the basic feed district that added ascorbic acid,, add the ascorbic acid of 2 mMs with respect to basic feed ingredient (in one kilogram).In the basic feed ingredient (in a kilogram), also add the L-ascorbic acid-2-magnesium phosphate of 2 mMs.Vitamin intermixture (5 gram) contains 6 milligrams of vitamin B ₁, 20 milligrams of vitamin B ₂, 4 milligrams of Cobastabs ₆Hydrochloride, 0.009 milligram of vitamin B ₁₂, 80 milligrams of nicotinic acid, 800 milligrams of Lipotrils, 400 milligrams of inositols, 28 milligrams of calcium pantothenates, 6 milligrams of vitamin H, 1.5 milligrams of acid, 40 milligrams of alpha-tocopherols, 4 milligrams of vitamin K ₃, 0.05 milligram of vitamin D ₂, 20 milligrams of letinene ac-etate and 3596 milligrams of celluloses.This standard feed sheet is suspended on the trout seedling raised 100 days.Fry 200 tails are put in each district.From first day to the 80 day, fed six every day, and from the 81 day to 100 days, fed four every day.The average weight of fish in each district is more or less the same.Move and grow the fry of adding L-ascorbic acid district, lethality reduces.Result according to the observation moves and grows no distortion of fry appearance of adding L-ascorbic acid-2-phosphoric acid magnesium salts district.(5pp)

SHOW?D-13?88-025158/04＝JP94093822.B2

The shell-fish fodder compound comprises ester or its salt of L-ascorbic acid-2-phosphoric acid

SHOWA?DENKO?KK?86.06.05?86JP-129283

C03(94.11.24)JP62285759-AA-23K/1/18./16.A16K31/375

According to JP62285759A

Its composition comprises the ester and the salt thereof of an amount of L-ascorbic acid-2-phosphoric acid

Purposes/effect: the not only nutrition but also economic composition (6pp) that are the growth of a kind of effective promotion shrimp and little cage shrimp

And/or the growth hormone polypeptides of fish, this polypeptide is to produce by the microorganism that recombinant dna plasmid is carried in cultivation, is disclosed in the Japanese patent application J94/095 of Kyowa Hakko Kogyo kk company, on 938, its summary is as follows:

KYOW?D16?86-008665/02＝JP94095938-B2

The microorganism of fish growth hormone polypeptide-carry recombinant dna plasmid by cultivation produces

KYOWA?HAKKO?KOGYO?KK?85.03.13?92?JP-204982(Div?ex?85.03.13?85JP-050096)

C03(94.11.30)EP?166444-A?C12N?15/18，C07K?13/100，C12N?1/21//A23K?1/165，A61K?35/74，37/36，C12P21/02(C12N1/21，C12R，1：19)(C12P?21/02，C12R1：19)

According to JP5207883-A

Additional data: SEKINE S (SEKI)

New fish growth hormone polypeptide (1) with disclosed peptide sequence.The DNA and the recombinant DNA of also claimed coding (1) and contain the microorganism of the recombinant DNA of coding (1).From the fish of Cupleiformes class, the m-RNA that isolates somatotropin as the pituitary gland of salmon is as template, preparation cRNA.The recombinant plasmid (as pS-GHI and pSGH14) of cDNA is mixed in preparation.This recombinant plasmid is incorporated in the host microorganism.This DNA and recombinant plasmid can be used for expressing the fish growth hormone gene in bacterium such as the colon bacillus.The microorganism that fish growth hormone carries recombinant plasmid by cultivation produces.

Purposes: (1) has effect that stimulates fish growth and the composition of culturing as fry.(11pp)

This literary composition is published in " the Derwent chemical patent index wall bulletin type digest " published on February 3rd, 1 on 95 year the 1st phase, and at least a material of (ii) aforementioned " ASIE " cause consumption and the concentration that shell-fish is excited and/or lure and/or excite and/or stimulate." ASIE " material can be with reference to preamble.

Equipment during practical operation of the present invention is to measure known substance, under constant or variable concentration of aqueous solution, and the equipment of the effect of whether cause at least a crustacean excitement, luring, excite and/or stimulate.Its equipment comprises, static groove equipment, circulation groove equipment and/or Y-type maze trough equipment.

More particularly, the present invention is with measuring known substance, whether causes that in known or variable concentration the device of shell-fish member excitation is carried out, and comprising:

(a) static groove equipment contains the water of (i) constant volume and (ii) at the salt solution of above-mentioned volume

Include the shell-fish of at least one work;

(b) pump into the equipment of experimental liquid,, will contain substances with variable or certain speed

Solution pump go into the equipment of static groove;

(c) produce the equipment of visible light and lead-in light, be about to variable or preferably constant strong

The light of the visible wavelength of degree imports the equipment of aforementioned static groove;

(d) first variable power supply is equipped, and is used to produce the equipment of visible light;

(e) second variable power supply is equipped, and is used to pump into the equipment of feed;

(f) video camera of variable-focus/focal length or Portable camcorder equipment.Work as use

Feed pumps into equipment and visible light produces when equipping, and can write down in the shell-fish a kind of

Or the motion and/or the health of a part of multiple member's health or special limbs

The lateral movement and the speed of a part or special limbs.

Can conclude in view of the above whether substances can cause crustacean excitation under specific dilution factor.

The video camera of variable-focus/focal length or Portable camcorder equipment are fit to use videotape to record on film and tape.

Another kind of useful device in the practice of the present invention can conclude whether known substance can lure shell-fish, excitement and excitation under concentration known or variable concentrations.This device comprises:

(a) circulation reservoir apparatus is testing liquid to be flowed into from inlet include crustacean water

In the groove, flow out from outlet again.This storage tank comprises that (i) mobile salt solution (ii) has at least

The shell-fish of a work is in the salt solution that flows;

(b) go into test feed solution equipment with infusion, will try with speed variable or that suit

Testing liquid injects in the circulation groove from the hole of front with pump;

(c) generation of visible light and guiding equipment, directs variable or suitable intensity as seen

Illumination is mapped in the interior circulating water volume of circulation storage tank;

(d) first variable power supply is used for to the equipment energy supply that produces visible light;

(e) a kind of variable power supply, to the equipment energy supply with pump input feed, pump has controllable

Pressure head, (this chemical solution contains at least a can to make the flow velocity of the chemical solution of adding

" ASIE "), reach in the basic brine stream that it can be added in the circulation groove;

(f) video camera of variable-focus/focal length or Portable camcorder equipment is used for note

In the shell-fish during equipment operation of employing pump feed equipment and producing visible light one

Kind or multiple member's motion and/or lateral movement and speed; With

(g) specially designed camera platform and shielding sheet equipment (part of the present invention) make

With purpose is in order to separate observer and experimental animal, experimental animal not to be done

Disturb and/or confuse, so that observe.(this pancratic video camera and video recorder

Be to be installed in specially designed platform and the shielding sheet).

The record equipment of this pancratic video camera or camcorder must make camera lens and shell-fish scope of activities be in line.Therefore, practical operation device of the present invention comprises a slice or two transparent panels, is camera or camcorder behind the plate, places before the camera to have crustacean mobile groove or static groove or Y-type maze trough.Camera gun can be strafed all crustacean scopes of activities.

In particular, help comprising at the video camera or the camcorder stage apparatus of the multi-direction rotation of variable focal length of crustacean active members and the visible wavelength message transmission between the image on for example visible wavelength photographic film or the tape shows thereof:

(1) one that is on the X-Y plane is the static plane bottom plate of rectangle, square substantially

The shape flat board has an end face, and has two substantially parallel ends parallel with X-axis, in addition

There are two substantially parallel ends parallel with Y-axis; A wherein parallel end with Y-axis

End then is along the straight line that is called " E1 ";

(2) being on the Y-Z plane another is the framework that stands on end of rectangle substantially, with

The above-mentioned static plane bottom plate approximate vertical that keeps flat.Two ends and the Y of framework

Axle is parallel, and other two is terminal parallel with the Z axle, the described end parallel with Y-axis

One of overlap with " E1 " line along first end with the base plate on described static plane,

Simultaneously parallel with Y-axis, described framework has first hole, can make visible wavelength

Light from then on see through;

The level of (3) continuous squares/upright plane bottom plate/framework movable platform can

Rotate to " X " and " Y " direction of principal axis (or side direction), comprise that (i) is at X-Y plane

On have a slice to be about the horizontal thin plate of rectangle, towards and in aforementioned horizontal stationary

In the plane of plane bottom plate (1).Thin plate has upper surface and lower surface, is continuously also

Sealing be abuttingly reposed against one parallel with " E1 " and be called as on the line of " E2 " the E1 line

Adjoining with the E2 line.(ii) a slice on the Y-Z plane is rectangle substantially

Vertical thin plate, towards and in aforementioned upright plane framework, (2) comprise one

The radiation shield of upright visible wavelength, on second hole is arranged, be seated in phase

Anti-and with the aforesaid upright framework with first hole in, promptly aforementioned it

The square movable platform is seated in the horizontal stationary base plate of axis of rolling direct neighbor

Top below vertical mobile platform;

(4) be the rotary bottom plate of rectangle substantially, a upper surface and lower surface are arranged.It is parallel

Both sides are parallel with Y-axis, and parallel in addition both sides are parallel with the X-Z face, can follow the top

Line E3 is parallel direction with hinge mode and E1 and E2 straight line and rotates, and at Y

Direction is parallel to the straight line E1 line and the E2 line of the first parallel hinged end of Y-axis

Joining, is a kind of hand operating tumbler, is seated in an end of rotational plane, with

The aforementioned relative side of the first parallel hinge end, with aforementioned E3 line position mutually

Instead, the direction of rotation of described rotating chassis is from described square movable platform

" X-Y " face turns to " Y-Z " of the square movable platform on top line E3 flat

Face.

(5) be contained in such rotation platform upper camera or camcorder, comprise a frame

Structure, the built-in camera camera lens is on the focal length plane pact of camera lens and the aforementioned vertical frame

First hole parallel, the controlled focal length is also with aforementioned visible long-wave radiation

Second hole is parallel on the shielding sheet, and promptly the camera lens and first hole and second hole have altogether

A sight line together.

Therefore, when operating aforementioned rotational plane base plate and aforementioned square rotatable platform simultaneously and using video camera or during camcorder, help (i) video camera or camcorder are taken, for example, the image on visible wavelength sensitive film or the sensitization tape show and (ii) the visible wavelength message between the image of moving target thing (referring to the shell-fish member herein) propagate on video camera or the camcorder.

Whether a certain material of operating another useful device of the present invention and be the concentration known that is determined in the aqueous solution or variable concentrations can lure or stimulate or excite the another kind equipment of shell-fish feed: this comprises " Y-type labyrinth type " device, the device that it and Lee are disclosed among " chemotaxis of a kind of cuttlefish in Y type maze trough device " (J.Exp.Mar.Biol.Ecol., Vol.153 (1992) p53-67) is similar.

More precisely, one a circulating groove that is equipped with Y-type labyrinth is equipped to realizing that the useful device of the present invention mainly comprises, its " 3 D stereo base portion " is rectangular parallelepiped protrusion part, has the arm of two rectangular parallelepiped protrusion part shapes; Its 3 D stereo base portion has the open end sealing of an end, in addition the two sides each be connected into and can seal with an end plate, outlet is in the open end, the parallelepiped of this outlet and two differences joins as described above, its drift angle is 5-45 ° (preferably drift angle is 10 °); (i) test article input area and (ii) reference standard district, each device joins with an end to be opened/closed and open end, also receives the 3 D stereo base portion of first rectangular parallelepiped protrusion part.Top of this device or two tops or bottom all are transparent plate, the camera system of an adjustable focal length transparent panel side therein so that take the crustacean activation record that might swim across.What must indicate is that (trier input pipe position is changed get final product) can be put upside down or exchange in the input port in test region or reference standard district.

Adding certain density test solution with a end and be advisable in the useful Y-type labyrinth type device of the present invention realizing from Y-type labyrinth.In the circulation groove device, in operating process, test solution concentration will constantly increase.

In addition, to realizing the useful Y-type labyrinth type device of the present invention, also comprise the water of a constant volume and contain the shell-fish of a work at least in this water capacity.The present invention's Y-type labyrinth type device comprises with infusion goes into experimental liquid, and input can different rates during experimental liquid, or preferably injects Y-type labyrinth with constant speed and feed groove (dividing one of limb with the parallelepiped-shaped that the parallelepiped of bottom joins).

This Y-type maze trough device as circulation groove device and static slot device, has the equipment of the light of a generation and visible wavelength directs variable or that be preferably constant intensity, light radiation is arrived raise in the shell-fish tank volume of living in.

To realizing that the useful Y-type maze trough device of the present invention also comprises zoom camera or video recorder record equipment.Under equipment of going into experimental liquid with infusion and the equipment that produces visible light move simultaneously, record shell-fish member's a part or all motions and/or the speed of one or more shell-fish lateral movements.

More precisely, when the response value " R " of measuring the shell-fish member or " G " on (this response value is the measurement that realizes result of the present invention), causing that shell-fish reacts the required time to the exciting agent of feeding or stimulant or attractant, is the used special article and the function of concentration and flow rate of liquid thereof.

The mathematical expression of drafting is as follows: And G ₂=100-10 θ (static slot device)

In this formula, θ refers to the required time, and " G1 " and " G2 " refer to when injecting reagent zero at first, to finding that the shell-fish member begins to make the response value when hastening forward motion.

What must indicate is that " G " herein also represents with " R ".

Response value change rate with respect to test solution concentration (C) (such as, in every liter of contained molal quantity or every liter of contained gram number) is expressed from the next: $\frac{d{G}_2}{\mathrm{dC}}=-10\[\frac{\mathrm{d\θ}}{\mathrm{dC}}\]$

Symbol herein:

Being the time change rate with respect to concentration, promptly is the reaction time, and symbol

Be reaction change rate with respect to concentration, as following formula:

Similar equation is: $\frac{d{G}_1}{\mathrm{dC}}=\frac{-100}{{\mathrm{\θ}}^2}\[\frac{\mathrm{d\θ}}{\mathrm{dC}}\]$

It is dependent on following mathematical expression: $G_1=\frac{100}{\mathrm{\θ}}-5$

In conjunction with mathematical expression and differential equation at first, the differential equation below promptly getting: $\frac{d{G}_1}{\mathrm{dC}}+G_1=\frac{100}{\mathrm{\θ}}-\frac{100}{{\mathrm{\θ}}^2}\[\frac{\mathrm{d\θ}}{\mathrm{dC}}\]-5$

With following differential equation: $\frac{d{G}_2}{\mathrm{dC}}+G_2=100-10\[\frac{\mathrm{d\θ}}{\mathrm{dC}}\]-10\mathrm{\θ}$

When stimulant concentration changed, response value was also along with change, or the concentration of change exciting agent or attractant, and response value is also along with change.Can show by following equation: $\mathrm{\ΔG}={\∫}_{C_1}^{C_2}\[\frac{\∂G}{\∂C}{\]}_{\mathrm{\θ}}\mathrm{dC};$ $\mathrm{\Δ}{G}_1={\∫}_{C_1}^{C_2}\[\frac{\∂G_1}{\∂C}{\]}_{\mathrm{\θ}}\mathrm{dC};$ With $\mathrm{\Δ}{G}_2={\∫}_{C_1}^{C_2}\[\frac{\∂G_2}{\∂C}{\]}_{\mathrm{\θ}}\mathrm{dC}$

Therefore, for example, if be used for chemical composition N-acetyl group-D-aminoglucose of the kind Penaens van-namei of crustacean shrimp genus, it is the mixture with two epimers of following structural formula:

Concentration can be shown by following formula :-log the relation of response value ₁₀C=0.8G-2.3 can following arbitrary formula show in static groove:

log ₁₀C＝2.3-0.8G；

0.8G＝2.3-log ₁₀C；

G＝2.9-1.25log ₁₀C；

G ₁＝2.9-1.25log ₁₀C；

G ₂=2.9-1.25log ₁₀C; With

G ₂＝2.9-2.9log _eC.

Furtherly, the reaction time change rate with respect to concentration can show by following formula: $\frac{\∂G_2}{\∂C}=\frac{-2.9}{C}$ With $\frac{\∂G_1}{\∂C}=\frac{-2.9}{C}$

And the change of reaction can show by following formula: $\mathrm{\Δ}{G}_1={\∫}_{C_1}^{C_2}\[\frac{-.29}{C}\]\mathrm{dC}=-2.9\mathrm{lo}{g}_e\[\frac{C_2}{C_1}\]$

In conjunction with aforesaid equation, can get following formula:

G ₁＝2.9-2.9log _eC

In conjunction with equation: $\frac{d{G}_1}{\mathrm{dC}}=\frac{-2.9}{C}$

Again in conjunction with equation: $\frac{d{G}_1}{\mathrm{dC}}+G_1=\frac{100}{\mathrm{\θ}}-\frac{100}{{\mathrm{\θ}}_2}\[\frac{\mathrm{d\θ}}{\mathrm{dC}}\]-5$

Promptly get equation: $\frac{-2.9}{C}+2.9-2.9{\log }_{e}C=\frac{100}{\mathrm{\θ}}-\frac{100}{{\mathrm{\θ}}^2}\[\frac{\mathrm{d\θ}}{\mathrm{dC}}\]-5$

(this formula shows that concentration is the function of time; When test compound constantly is added into circulation groove or static groove rather than Y-type maze trough device) its equation is as follows: $7.9-2.9\[\frac{1}{C}+{\log }_{e}C\]=100\[\frac{1}{\mathrm{\θ}}-\frac{1}{{\mathrm{\θ}}^2}\[\frac{\mathrm{d\θ}}{\mathrm{dC}}\]\]$

When operations flows groove device, and shell-fish is when selecting the kind Penaeus van-namei that shrimp belongs to for use, and with N-acetyl group-D-aminoglucose, it is the mixture of two following epimers of structural formula:

During as analeptic, attractant, stimulant and/or exciting agent (the usage amount ratio is 0-50, is used for static groove and then is 1-5), the average response value is the function of N-acetyl group-D-aminoglucose concentration, and its mathematical expression is as follows: $R=51.06+\frac{16.14}{{\log }_{10}C+11.04}$

Or $R=100{\log }_e\[\frac{30-{\log }_{10}C}{30}\]$

The intermediate reaction value is the function of test compound N-acetyl group-D-aminoglucose concentration, and its mathematical expression is as follows:

R＝-41.98-5.68log ₁₀C.

Other was once tested but the material that do not constitute the present invention's a part has following several:

(i) taurine, its structural formula is as follows:

(ii) guanidine, its structural formula is as follows:

(iii) betain, its structural formula is as follows:

(iv) citric acid, its structure is as follows:

(v) pyruvic acid, its structure is as follows:

(vi) forulic acid, its structural formula is as follows:

(vii) vanillin, its structural formula is as follows:

(viii) isopentyl aldehyde, its structural formula is as follows:

(ix) trans-2-hexenoic aldehyde, its structure is as follows:

(x) citric acid, its structural formula is as follows:

Proof each material of the present invention institute is all with the saline solution dilution, and this saline solution contains " FRITZ ^" super salt concentrate, concentration is 30/1000.This salt solution is the FRITZ company product that texas,U.S is answered the Lars city.This salt aqueous concentrate contains sodium chloride, magnesium sulfate, magnesium chloride, calcium chloride and a spot of lithium chloride, sodium molybdate, disodium hydrogen phosphate, strontium chloride, potassium chloride, sodium bicarbonate, calcium carbonate and the magnesium carbonate of main amount.

The aforementioned water capacity that contains shell-fish is the weak brine solution of the FRITZ salt solution of 30/1000 concentration that contains mentioned component.

Reaction time is that the mathematical expression of the function of activity chemistry thing (as TMOH, NAG, OBS and/or MOS, be used alone or in combination, be used for the cray kind Procambrus claekii of shell-fish) concentration can be expressed as follows: $R=5.0014-\[\mathrm{lo}{g}_{10}C{\]}^2(0.0084-0.5169e^{+0.45\left({\log }_{10}C\right)})$

(mean value when using NAG);

R=0.7874[-log ₁₀C] ^-3/2-0.0001[log ₁₀C] ²+3.041

(median when using NAG);

R=4.6574+0.0018[log ₁₀C] ³+0.0322[log ₁₀C] ²+0.0907[log ₁₀C]

(mean value at sum strong point when using NAG); $R=(-6\×{10}^{-4}){{\left[{\log }_{10}C\right]}_e}^{-0.2596\left[{\log }_{10}C\right]}+0.0207\left[{\log }_{10}C\right]+3.5607$

(mean value when using TMOH);

R=-2.6824?sinh[-0.1{log ₁₀C}]-0.3680[log ₁₀C]+3.1913

(median of the data point when using TMOH); $R=(-0.0082)\[{\log }_{10}C{\]}^2{e}^{0.0573\left({\log }_{10}C\right)}-0.0066\[{\log }_{10}C{\]}^2+6.3210$

(mean value of the data point when using OBS); $R=(-1.5542\×{10}^4)\{{\log }_{10}C{\}}^2{e}^{+2.1941\left({\log }_{10}C\right)}-0.1613\[{\log }_{10}C{\]}^2+11.2387$

(median of the data point when using OBS); $R=0.1302\[{\log }_{10}C{\]}^2{e}^{0.092\left({\log }_{10}C\right)}-0.3697\[{\log }_{10}C\]-5.0549$

(mean value of the data point when using MOS); With $R=152.9674-0.732\[{\log }_{10}C{\]}^2{e}^{0.048{\log }_{10}C}+12.433\[{\log }_{10}C\]$

(median of the data point when using MOS).

In the aforementioned equation, the R item is the counting of aforementioned " reaction ", and the C item is the concentration of the chemical composition of effect, and for example the concentration of OBS in salt solution is represented with the gram mol.

Claims (3)

1. one kind is attracted to method with the desirable water surface or waters the sheet of water with shellfish from its waters of perching, comprises being applied near the above-mentioned desirable water surface or the waters step with containing the aqueous solution that is selected from least a the attracted shellfish concentration in the following compound:

(I) N-acetyl group-D-aminoglucose is the mixture of two kinds of epimers with following structure;

(II) has the S-methylmethionine halogenation sulfonium of following general formula;

Wherein, X is cl anion or bromine anions;

(III) methionine, its structural formula is as follows:

(IV) have the trimethylamine-N-oxide dihydrate of following structural formula:

(V) 1-octene-3-alcohol, its structural formula is as follows:

It is the mixture of two kinds of isomers, and its structural formula is as follows: (VI) 3-(methylthio) propanal, its structural formula is as follows: (VII) methyl-sulfoxide, its structural formula is as follows:

(VII) 50: 50 mole: the scatol of molar ratio/indoles mixture, its structure

Formula is as follows:

Scatol: indoles:

(IX) S-methyl first sulphur propionic acid halogenation sulfonium, its structural formula is as follows:

Wherein X is cl anion or bromine anions; (X) ammonium chloride; (XI) ammonium acetate; (XII) acetic acid; (XIII) glucose (XIV) raw sugar is the mixture of sucrose and impurity; (XV) Thaumatin; (XVI) 2-methyl-3-(methyl two sulphur) furans, its structural formula is as follows: (XVII) ammoniacal liquor; (XVIII) yeast extract; (XIX) D-aminoglucose is two and the mixture of isomeric compound, and its structural formula is as follows:

(XX) 2-methyl-3-furanthiol, its structural formula is as follows:

2. one kind makes shellfish cause excitement, the method that excites and/or stimulate in the waters that it is perched, and comprises being applied near the waters that described shell-fish perches step with closing the aqueous solution that is selected from the concentration at least a, that cause the shellfish excitement, excite and/or stimulate in the following material:

(I) N-acetyl group-D-aminoglucose is the mixture of two kinds of epimers with following structure;

(II) has the S-methylmethionine halogenation sulfonium of following general formula; Wherein, X is cl anion or bromine anions; (III) methionine, its structural formula is as follows: (IV) have the trimethylamine-N-oxide dihydrate of following structural formula:

(V) 1-octene-3-alcohol, its structural formula is as follows: It is the mixture of two kinds of isomers, and its structural formula is as follows:

(VI) 3-(methylthio) propanal, its structural formula is as follows:

(VII) methyl-sulfoxide, its structural formula is as follows:

(VII) scatol: indoles with 50: 50 the mole: the mixture of molar ratio, its structural formula is as follows:

Scatol: indoles:

(IX) S-methyl first sulphur propionic acid halogenation sulfonium, its structural formula is as follows:

Wherein X is cl anion or bromine anions; (X) ammonium chloride; (XI) ammonium acetate; (XII) acetic acid; (XIII) glucose (XIV) raw sugar is the mixture of sucrose and impurity; (XV) Thaumatin; (XVI) 2-methyl-3-(methyl two sulphur) furans, its structural formula is as follows:

(XVII) ammoniacal liquor; (XVIII) yeast extract; (XIX) D-aminoglucose is two and the mixture of isomeric compound, and its structural formula is as follows:

(XX) 2-methyl-3-furanthiol, its structural formula is as follows:

3. fortified feed composition that is used for shellfish, comprise that (I) basic feed ingredient and well-mixed with it (II) generation lure and/or stimulate and/or excite and/or the concentration of excitation and the feed enhancement material of amount, this material can be selected from least a in following each thing:

(I) N-acetyl group-D-aminoglucose is the mixture of two kinds of epimers with following structure;

(II) has the S-methylmethionine halogenation sulfonium of following general formula; Wherein, X is cl anion or bromine anions; (III) methionine, its structural formula is as follows: (IV) have the trimethylamine-N-oxide dihydrate of following structural formula:

(V) 1-octene-3-alcohol, its structural formula is as follows:

It is the mixture of two kinds of isomers, and its structural formula is as follows:

(VI) 3-(methylthio) propanal, its structural formula is as follows:

(VII) methyl-sulfoxide, its structural formula is as follows:

(VIII) scatol: indoles with 50: 50 the mole: the mixture of molar ratio, its structural formula is as follows:

Scatol: indoles:

(IX) S-methyl first sulphur propionic acid halogenation sulfonium, its structural formula is as follows: Wherein X is cl anion or bromine anions; (X) ammonium chloride; (XI) ammonium acetate; (XII) acetic acid; (XIII) glucose (XIV) raw sugar is the mixture of sucrose and impurity; (XV) Thaumatin; (XVI) 2-methyl-3-(methyl two sulphur) furans, its structural formula is as follows:

(XVII) ammoniacal liquor; (XVIII) yeast extract; (XIX) D-aminoglucose is the mixture of two kinds of isomeric compounds, and its structural formula is as follows:

(XX) 2-methyl-3-furanthiol, its structural formula is as follows: