CN116286312A - Nucleic acid detection kit and detection method thereof - Google Patents

Nucleic acid detection kit and detection method thereof Download PDF

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Publication number
CN116286312A
CN116286312A CN202310199122.7A CN202310199122A CN116286312A CN 116286312 A CN116286312 A CN 116286312A CN 202310199122 A CN202310199122 A CN 202310199122A CN 116286312 A CN116286312 A CN 116286312A
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CN
China
Prior art keywords
gas
liquid flow
bin
hole
nucleic acid
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Pending
Application number
CN202310199122.7A
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Chinese (zh)
Inventor
吴菊
钟唐
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Suzhou Ke'er Life Technology Co ltd
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Suzhou Ke'er Life Technology Co ltd
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Priority to CN202310199122.7A priority Critical patent/CN116286312A/en
Publication of CN116286312A publication Critical patent/CN116286312A/en
Pending legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D43/00—Lids or covers for rigid or semi-rigid containers
    • B65D43/14—Non-removable lids or covers
    • B65D43/16—Non-removable lids or covers hinged for upward or downward movement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • B01D46/54—Particle separators, e.g. dust precipitators, using ultra-fine filter sheets or diaphragms
    • B01D46/543—Particle separators, e.g. dust precipitators, using ultra-fine filter sheets or diaphragms using membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D51/00—Closures not otherwise provided for
    • B65D51/16—Closures not otherwise provided for with means for venting air or gas
    • B65D51/1672—Closures not otherwise provided for with means for venting air or gas whereby venting occurs by manual actuation of the closure or other element
    • B65D51/1683—Closures not otherwise provided for with means for venting air or gas whereby venting occurs by manual actuation of the closure or other element by actuating a separate element in the container or closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
    • B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
    • B65D81/2007—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas under vacuum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention relates to a nucleic acid detection kit and a detection method thereof, comprising the following steps: the box body, the box body includes: the rotary piston fixing groove is horizontally arranged at the bottom of the second side wall of the box body; a plurality of gas-liquid flow holes, the gas-liquid flow holes comprising a first gas-liquid flow hole, a second gas-liquid flow hole; according to the nucleic acid detection kit and the application method thereof, the rotatable rotary piston is arranged, so that the negative pressure bin can be vacuumized in advance during production, and the nucleic acid sample treatment liquid can be automatically driven to circulate in the device through negative pressure during use, so that the nucleic acid detection kit is simple in structure and convenient to operate; by arranging the reaction bin and the discharging gas-liquid flow channel, gas in the sample treatment liquid can be separated out, and the influence of the gas on the observation of a detection result is avoided; the filter membrane and the discharging filter membrane are further arranged, so that the sample treatment fluid and the exhaust gas can be filtered, the detection precision is improved, and aerosol pollution is avoided.

Description

Nucleic acid detection kit and detection method thereof
Technical Field
The invention relates to the technical field of medical instruments, in particular to a nucleic acid detection kit and a detection method thereof.
Background
Nucleic acid detection is widely used in various fields, and particularly widely popularized in biomedical diagnosis technology, including pathogenic microorganism detection, disease diagnosis and the like.
The existing nucleic acid detection technology generally uses a centrifugal column method or a magnetic bead method to extract nucleic acid from a sample, and generally requires steps such as cracking, binding, washing, eluting and the like. The process is complex and complicated in operation, in order to simplify the process, an open extraction kit is developed on the market, and the existing open extraction kit is extremely easy to cause aerosol pollution in the use process, so that the detection result is inaccurate.
Therefore, there is a need for a nucleic acid detection kit and a detection method thereof that are simple to operate and reduce aerosol contamination.
Disclosure of Invention
The invention aims at overcoming the defects in the prior art and provides a nucleic acid detection kit and a detection method thereof.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
in a first aspect, the present invention provides a nucleic acid detection kit comprising:
the box body, the box body includes:
the sample treatment bin is arranged on the first side of the interior of the box body;
the negative pressure bin is arranged on the second side of the interior of the box body, and a baffle plate is fixedly arranged between the sample treatment bin and the negative pressure bin;
the rotary piston fixing groove is horizontally arranged at the bottom of the second side wall of the box body;
and a plurality of gas-liquid flow holes including a first gas-liquid flow hole vertically penetrating the bottom of the negative pressure cartridge and the rotary piston fixing groove to the first side bottom of the cartridge body, and a second gas-liquid flow hole vertically penetrating the bottom of the sample processing cartridge and the rotary piston fixing groove to the first side bottom of the cartridge body;
the rotary piston is rotationally arranged in the rotary piston fixing groove;
and a reaction tube detachably provided in the bottom of the case, the reaction tube including:
the freeze-dried ball storage position is arranged on the first side of the inside of the reaction tube, and when the rotary piston is communicated with the first gas-liquid flow through hole, the top of the freeze-dried ball storage position is communicated with the bottom of the first gas-liquid flow through hole;
and the reaction bin is arranged at the bottom of the reaction tube, and the cross section of the reaction bin is prismatic.
Further, the box body further includes:
the flip cover is fixedly arranged at the top of the box body through a connecting seat, and is rotationally arranged at the far-end edge of the connecting seat;
the welding line is arranged at the top edge of the box body and is fixedly connected with the bottom of the connecting seat;
the piston limiting boss is fixedly arranged outside the rotary piston fixing groove;
and the locking hook is fixedly arranged in the middle of the bottom side of the box body.
Further, the sample processing cartridge comprises:
the filtering membrane fixing groove is formed in the bottom of the sample treatment bin;
and a filtering membrane fixedly arranged in the filtering membrane fixing groove.
Further, the flip cover includes:
the sample injection hole is formed in the first side of the surface of the connecting seat and is communicated with the top of the sample treatment bin;
the sealing boss is fixedly arranged on the first side of the inner part of the flip cover and is matched with the sample injection hole; the sealing boss includes:
the air hole is formed in the center of the sealing boss;
the filter membrane installation position is arranged in the sealing boss;
the sample injection filter membrane is fixedly arranged in the filter membrane positioning position;
the buckling bulge is fixedly arranged at the edge of the proximal end of the fixing seat;
the groove is formed in the edge of the proximal end of the flip cover, and the buckling protrusion is matched with the groove;
and the fixing seat welding line is arranged at the bottom edge of the fixing seat. Further, the rotary piston includes:
the main body is rotatably arranged in the rotary piston fixing groove;
a soft layer provided on an outer wall of the main body;
the rotary wrench is vertically and fixedly arranged at the second end of the main body, and is matched with the piston limiting boss;
the first connecting hole is formed in the outer wall of the first side of the main body along the direction perpendicular to the horizontal plane, and is communicated with the first gas-liquid flow through hole;
and the second connecting hole is formed in the outer wall of the second side of the main body along the direction vertical to the horizontal plane, the second connecting hole is communicated with the second gas-liquid flow through hole, and the first connecting hole and the second connecting hole are vertical to the rotary wrench.
Further, the reaction tube includes:
the bin inlet gas-liquid flow channel is arranged at the bottom of the freeze-drying ball storage position, and the top of the bin inlet gas-liquid flow channel is communicated with the bottom of the freeze-drying ball storage position;
the discharging gas-liquid flow passage is arranged at the top of the reaction bin
The drying agent storage position is arranged on the second side of the inside of the reaction tube, and when the rotary piston is communicated with the first gas-liquid flow through hole, the top of the drying agent storage position is communicated with the bottom of the second gas-liquid flow through hole;
the filtering membrane placement position is arranged at the bottom of the drying agent storage position;
the discharging filter membrane is fixedly arranged in the filter membrane placing position;
the locking hook clamping position is arranged in the middle of the inner side of the reaction tube and is matched with the locking hook;
and the two groups of films are fixedly arranged on the bottom proximal side wall and the distal side wall of the reaction tube respectively.
Further, the intake air-liquid flow path includes:
the triangular bosses are uniformly arranged on the inner wall of the gas-liquid flow passage of the inlet bin.
Further, the reaction bin includes:
the two upper edges are fixedly arranged on the upper side of the reaction bin;
the right-angle sawtooth structures are uniformly arranged on the inner walls of the two upper edges of the reaction bin;
and the two lower edges are fixedly arranged at the lower side of the reaction bin.
Further, the top end of the discharging gas-liquid runner is communicated with the bottom of the filter membrane placement position.
In a second aspect, the present invention provides a method for detecting a nucleic acid detection kit, comprising the steps of:
s1, opening a flip cover, injecting a nucleic acid sample and sample treatment fluid from a sample injection hole, closing the flip cover, and rotating a rotary wrench to be parallel to a horizontal plane, wherein the gas-liquid flow hole is in a communication state;
s2, the processed sample liquid passes through a filtering membrane, enters a gas-liquid flow through hole, passes through a first connecting hole in the middle of a rotary piston, enters a freeze-drying ball storage position of a reaction tube, and redissolves preset freeze-drying balls;
s3, the sample solution enters and fills the reaction bin through a bin inlet gas-liquid flow channel of the reaction tube, gas discharged in a squeezing way in the liquid flowing process enters the negative pressure bin through a bin outlet gas-liquid flow channel and a bin outlet filtering membrane under the negative pressure effect of the negative pressure bin through a second connecting hole in the middle of the rotary piston;
s4, rotating the rotary wrench to a vertical position, wherein the gas-liquid communication hole is in a sealed state at the moment, maintaining the air pressure balance, and inserting the kit into a detection instrument for nucleic acid detection.
Compared with the prior art, the invention has the following technical effects:
according to the nucleic acid detection kit and the detection method thereof, the rotatable rotary piston is arranged, so that the negative pressure bin can be vacuumized in advance during production, and the nucleic acid sample treatment liquid can be automatically driven to circulate in the device through negative pressure during use, so that the nucleic acid detection kit is simple in structure and convenient to operate; by arranging the reaction bin and the discharging gas-liquid flow channel, gas in the sample treatment liquid can be separated out, and the influence of the gas on the observation of a detection result is avoided; the filter membrane and the discharging filter membrane are further arranged, so that the sample treatment fluid and the exhaust gas can be filtered, the detection precision is improved, and aerosol pollution is avoided.
Drawings
FIG. 1 is an exploded view of a nucleic acid detecting kit according to an example of the present invention;
FIG. 2 is a schematic diagram showing the closed and connected states of a nucleic acid detecting kit according to an embodiment of the present invention;
FIG. 3 is a schematic diagram showing the structure of a flip cover of a nucleic acid detecting kit according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a nucleic acid detecting kit according to an embodiment of the present invention;
FIG. 5 is a schematic diagram showing the structure of a rotary piston of a nucleic acid detecting kit according to an embodiment of the present invention;
FIG. 6 is a schematic diagram showing the structure of a reaction tube of a nucleic acid detecting kit according to an example of the present invention;
wherein the first side is disposed opposite the second side, and reference numerals include:
a flip cover 1; a sample inlet 11; a sealing boss 12; an air hole 121; a filter membrane placement bit 122; sampling a filtering membrane; a buckling protrusion 13; a groove 14; a holder weld line 15; a case 2; a weld line 21; a sample processing cartridge 22; a filtering membrane fixing groove 221; a filter membrane 222; a negative pressure bin 23; gas-liquid flow through hole 24; a rotary piston fixing groove 25; a piston limit boss 26; a locking hook 27; a rotary piston 3; a soft layer 31; a first connection hole 321; a second connection hole 322; a rotating spanner 33; a reaction tube 4; freeze-dried pellet storage location 41; freeze-dried balls 411; a feed gas-liquid flow path 42; triangular boss 421; a reaction chamber 43; right angle sawtooth structures 431; a lower edge 432; a discharge gas-liquid flow path 44; a filter membrane placement site 45; a discharge filtration membrane 451; a desiccant holder 46; a drying agent 461; a locking hook catch 47; a film 48.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other.
The invention is further described below with reference to the drawings and specific examples, which are not intended to be limiting.
Example 1
Referring to fig. 1, the present embodiment provides a nucleic acid detection kit, which includes:
a case 2; referring to fig. 2 to 6, the case 2 includes:
a case 2, the case 2 comprising:
the rotary piston fixing groove 25 is horizontally arranged at the bottom of the second side wall of the box body 2;
a plurality of gas-liquid flow holes 24, the gas-liquid flow holes 24 comprising a first gas-liquid flow hole, a second gas-liquid flow hole;
a piston limit boss 26, wherein the piston limit boss 26 is fixedly arranged outside the rotary piston fixing groove 25;
a weld line 21, the weld line 21 being provided at a top edge of the case 2;
the sample treatment bin 22 is arranged on the first side of the interior of the box body 1, and a sample treatment liquid is arranged in the sample treatment bin 22; the sample processing cartridge 22 includes:
a filtering membrane fixing groove 221, wherein the filtering membrane fixing groove 221 is arranged at the bottom of the sample treatment bin 22;
and a filter membrane 222 fixedly provided inside the filter membrane fixing groove 221;
the negative pressure bin 23 is arranged on the second side of the interior of the box body 2, a partition plate is fixedly arranged between the sample treatment bin 22 and the negative pressure bin 23, a first gas-liquid flow hole 24 vertically penetrates through the bottom of the negative pressure bin 23 and the rotary piston fixing groove 25 to extend to the bottom of the first side of the box body 2, and a second gas-liquid flow hole 24 vertically penetrates through the bottom of the sample treatment bin 22 and the rotary piston fixing groove 25 to extend to the bottom of the first side of the box body 2;
and a locking hook 27, the locking hook 27 being fixedly provided at a bottom side middle portion of the case 1.
Flip 1, flip 1 pass through the connecting seat fixedly set up in the top of box body 2, flip 1 rotationally set up in the distal end border of connecting seat, flip 1 includes:
the sample injection hole 11 is formed in the first side of the surface of the connecting seat, and the sample injection hole 11 is communicated with the top of the sample treatment bin 22;
the sealing boss 12 is fixedly arranged on the first side of the inner part of the flip cover 1, and the sealing boss 12 is matched with the sample injection hole 11; the sealing boss 12 includes:
an air hole 121, wherein the air hole 121 is arranged at the center of the sealing boss 12;
a filter membrane setting point 122, wherein the filter membrane setting point 122 is arranged inside the sealing boss 12;
the sample injection filter membrane 123 is fixedly arranged in the filter membrane placement position 122;
the buckling bulge 13 is fixedly arranged at the edge of the proximal end of the fixing seat; the groove 14 is formed in the edge of the proximal end of the flip cover 1, and the buckling protrusion 13 is matched with the groove 14;
and a fixing seat welding line 15, wherein the fixing seat welding line 15 is arranged at the bottom edge of the fixing seat.
A rotary piston 3, the rotary piston 3 is rotatably provided in the rotary piston fixing groove 25, and the rotary piston 3 includes:
a main body rotatably provided inside the rotary piston fixing groove 25;
a soft layer 31 provided on an outer wall of the main body;
a rotary wrench 33, wherein the rotary wrench 33 is vertically and fixedly arranged at the second end of the main body, and the rotary wrench 33 is matched with the piston limiting boss 26;
a first connection hole 321, wherein the first connection hole 321 is opened on the outer wall of the first side of the main body along the direction vertical to the horizontal plane, and the first connection hole 321 is communicated with the first gas-liquid flow through hole 24;
and a second connecting hole 322, wherein the second connecting hole 322 is opened on the second side outer wall of the main body along the direction vertical to the horizontal plane, the second connecting hole 322 is communicated with the second gas-liquid flow hole 24, and the first connecting hole 321 and the second connecting hole 322 are vertical to the rotating wrench 33;
and a reaction tube 4, wherein the reaction tube 4 is detachably arranged at the bottom of the box body 2; the reaction tube 4 includes:
a freeze-dried ball storage place 41, wherein the freeze-dried ball storage place 41 is arranged on the first side of the inside of the reaction tube 4, and the top of the freeze-dried ball storage place 41 is communicated with the bottom of the gas-liquid flow hole 24 on the first side;
a freeze-dried ball 411, wherein the freeze-dried ball 411 is arranged inside the freeze-dried ball storage place 41;
a bin inlet gas-liquid flow passage 42, wherein the bin inlet gas-liquid flow passage 42 is arranged at the bottom of the freeze-dried ball storage position 41, and the top of the bin inlet gas-liquid flow passage 42 is communicated with the bottom of the freeze-dried ball storage position 41; by a means of
The inlet gas-liquid flow path 42 includes:
the triangular bosses 421 are uniformly arranged on the inner wall of the air-liquid channel 42, and form an accelerating vortex for the inlet liquid and a resistance for the reverse liquid flow by a Tesla valve channel principle;
the reaction bin 43 is arranged at the bottom of the reaction tube 4, and the cross section of the reaction bin 43 is prismatic; the reaction chamber 43 includes:
two upper edges fixedly arranged on the upper side of the reaction chamber 43;
the right-angle sawtooth structures 431 are uniformly arranged on the inner walls of the two upper edges of the reaction bin 43 and used for reflecting light rays and improving fluorescence excitation efficiency;
and two lower edges 432, wherein the two lower edges 432 are fixedly arranged at the lower side of the reaction chamber 43;
a discharging gas-liquid flow channel 44, wherein the discharging gas-liquid flow channel 44 is arranged at the top of the reaction bin 43, and the top end of the discharging gas-liquid flow channel 44 is communicated with the bottom of the filtering membrane placement position 45;
a desiccant storage location 46, wherein the desiccant storage location 46 is arranged on the second side of the interior of the reaction tube 4, and the top of the desiccant storage location 46 is communicated with the bottom of the gas-liquid flow hole 24 on the second side;
a desiccant 461, wherein the desiccant 461 is arranged inside the desiccant storage position 46;
a filter membrane placement site 45, wherein the filter membrane placement site 45 is arranged at the bottom of the desiccant storage site 46;
a discharge filter membrane 451, wherein the discharge filter membrane 451 is fixedly disposed inside the filter membrane placement site 45;
the locking hook clamping position 47 is arranged at the middle part of the inner side of the reaction tube 4, and the locking hook clamping position 47 is matched with the locking hook 27;
and two groups of films 48, wherein the two groups of films 48 are fixedly arranged on the proximal side wall and the distal side wall of the bottom of the reaction tube 4 respectively.
Example 2
The embodiment provides a detection method of a nucleic acid detection kit, which comprises the following steps:
s1, welding and fixing the flip cover 1 and the box body 2 together, wherein the sealing performance of the device can be improved by the welding line 15 and the welding line 21 of the fixed seat;
s2, inserting the first end of the rotary piston 3 into the rotary piston fixing groove 25, rotating the rotary wrench 33 to be parallel to the horizontal plane, enabling the gas-liquid flow hole 24 to be in a communicated state, connecting the gas-liquid communication hole 24 at the lower end of the negative pressure bin 23 of the box body 2 by using a vacuum pump suction gun head, and starting to suck air from the negative pressure bin 23 after inputting the required pressure on the vacuum pump to form a negative pressure state;
s3, rotating the rotary wrench 33 to a vertical position, wherein the gas-liquid communication hole 24 is in a sealed state;
s4, clamping the locking hooks 27 into the locking hook clamping positions 47 so that the box body 2 and the reaction tube 4 are connected together;
s5, during detection, opening the flip cover 1, injecting a nucleic acid sample and a sample treatment liquid from the sample injection hole 11, closing the flip cover 1, and rotating the rotary wrench 33 to be parallel to the horizontal plane, wherein the gas-liquid flow hole 24 is in a communication state;
s6, the processed sample liquid passes through the filtering membrane 222, enters the gas-liquid flow through hole 24, passes through the first connecting hole 321 in the middle of the rotary piston 3, enters the freeze-drying ball storage position 41 of the reaction tube 4, and redissolves the preset freeze-drying ball 411;
s7, the sample solution enters and fills the reaction bin 43 through the bin inlet gas-liquid flow channel 42 of the reaction tube 4, the gas discharged in the liquid flowing process is extruded, and under the negative pressure effect of the negative pressure bin 23, aerosol pollution is prevented through the bin outlet gas-liquid flow channel 44 and the bin outlet filtering membrane 451, and the sample solution enters the negative pressure bin 23 through the second connecting hole 322 in the middle of the rotary piston 3;
s8, rotating the rotary wrench 33 to the vertical position, wherein the gas-liquid communication hole 24 is in a sealed state, maintaining the air pressure balance, and inserting the kit into a detection instrument for nucleic acid detection.
In summary, according to the nucleic acid detection kit and the detection method thereof, the rotatable rotary piston 3 is arranged, so that the negative pressure bin 23 can be vacuumized in advance during production, and the nucleic acid sample treatment liquid can be automatically driven to circulate in the device through negative pressure during use, so that the nucleic acid detection kit has a simple structure and is convenient to operate; by arranging the reaction bin 43 and the discharging gas-liquid channel 44, gas in the sample treatment liquid can be separated out, and the influence of the gas on the observation of the detection result is avoided; the filter membrane 222 and the discharging filter membrane 451 are also arranged, so that the sample treatment liquid and the exhaust gas can be filtered, the detection precision is improved, and aerosol pollution is avoided.
The foregoing description is only illustrative of the preferred embodiments of the present invention and is not to be construed as limiting the scope of the invention, and it will be appreciated by those skilled in the art that equivalent substitutions and obvious variations may be made using the description and illustrations of the present invention, and are intended to be included within the scope of the present invention.

Claims (10)

1. A nucleic acid detection kit comprising:
box body (2), box body (2) include:
the sample treatment bin (22) is arranged on the first side of the interior of the box body (1);
the negative pressure bin (23), the negative pressure bin (23) is arranged on the second side of the interior of the box body (2), and a baffle is fixedly arranged between the sample treatment bin (22) and the negative pressure bin (23);
the rotary piston fixing groove (25) is horizontally formed in the bottom of the second side wall of the box body (2);
and a plurality of gas-liquid flow holes (24), the gas-liquid flow holes (24) including a first gas-liquid flow hole and a second gas-liquid flow hole, the first gas-liquid flow hole (24) vertically penetrating the bottom of the negative pressure chamber (23) and the rotary piston fixing groove (25) extending to the first side bottom of the cartridge body (2), the second gas-liquid flow hole (24) vertically penetrating the bottom of the sample processing chamber (22) and the rotary piston fixing groove (25) extending to the first side bottom of the cartridge body (2);
a rotary piston (3), wherein the rotary piston (3) is rotatably arranged in the rotary piston fixing groove (25);
and a reaction tube (4), the reaction tube (4) being detachably provided in the bottom of the case body (2), the reaction tube (4) comprising:
the freeze-dried ball storage position (41), the freeze-dried ball storage position (41) is arranged on the first side of the inside of the reaction tube (4), and when the rotary piston (3) is communicated with the first gas-liquid flow hole (24), the top of the freeze-dried ball storage position (41) is communicated with the bottom of the first gas-liquid flow hole (24);
and the reaction bin (43), the reaction bin (43) is arranged at the bottom of the reaction tube (4), and the cross section of the reaction bin (43) is prismatic.
2. The nucleic acid detection kit according to claim 1, wherein the cartridge body (2) further comprises:
the flip cover (1) is fixedly arranged at the top of the box body (2) through a connecting seat, and the flip cover (1) is rotationally arranged at the far-end edge of the connecting seat;
the welding line (21) is arranged at the top edge of the box body (2), and the welding line (21) is fixedly connected with the bottom of the connecting seat;
a piston limit boss (26), wherein the piston limit boss (26) is fixedly arranged outside the rotary piston fixing groove (25);
and a locking hook (27), wherein the locking hook (27) is fixedly arranged at the middle part of the bottom side of the box body (1).
3. The nucleic acid detection kit according to claim 1, wherein the sample processing cartridge (22) comprises:
the filtering membrane fixing groove (221), the filtering membrane fixing groove (221) is arranged at the bottom of the sample treatment bin (22);
and a filtration membrane (222) fixedly provided inside the filtration membrane fixing groove (221).
4. The nucleic acid detection kit according to claim 2, characterized in that the flip (1) comprises:
the sample injection hole (11), the sample injection hole (11) is arranged on the first side of the surface of the connecting seat, and the sample injection hole (11) is communicated with the top of the sample treatment bin (22);
the sealing boss (12) is fixedly arranged on the first side of the inside of the flip (1), and the sealing boss (12) is matched with the sample injection hole (11); the sealing boss (12) includes:
an air hole (121), wherein the air hole (121) is arranged in the center of the sealing boss (12);
a filter membrane setting bit (122), wherein the filter membrane setting bit (122) is arranged inside the sealing boss (12);
the sample injection filter membrane (123) is fixedly arranged in the filter membrane setting position (122);
the buckling bulge (13) is fixedly arranged at the edge of the proximal end of the fixing seat;
the groove (14) is formed in the edge of the proximal end of the flip (1), and the buckling protrusion (13) is matched with the groove (14);
and the fixing seat welding line (15), wherein the fixing seat welding line (15) is arranged at the bottom edge of the fixing seat.
5. The nucleic acid detection kit according to claim 1, characterized in that the rotary piston (3) comprises:
a main body rotatably provided inside the rotary piston fixing groove (25);
a soft layer (31) provided on the outer wall of the main body;
a rotary wrench (33), wherein the rotary wrench (33) is vertically and fixedly arranged at the second end of the main body, and the rotary wrench (33) is matched with the piston limiting boss (26);
a first connecting hole (321), wherein the first connecting hole (321) is arranged on the outer wall of the first side of the main body along the direction vertical to the horizontal plane, and the first connecting hole (321) is communicated with the first gas-liquid flow through hole (24);
and a second connecting hole (322), wherein the second connecting hole (322) is formed in the outer wall of the second side of the main body along the direction vertical to the horizontal plane, the second connecting hole (322) is communicated with the second gas-liquid flow through hole (24), and the first connecting hole (321) and the second connecting hole (322) are vertical to the rotating spanner (33).
6. The nucleic acid detection kit according to claim 1, wherein the reaction tube (4) further comprises:
the bin inlet gas-liquid flow channel (42), the bin inlet gas-liquid flow channel (42) is arranged at the bottom of the freeze-drying ball storage position (41), and the top of the bin inlet gas-liquid flow channel (42) is communicated with the bottom of the freeze-drying ball storage position (41);
an outlet gas-liquid flow passage (44), the outlet gas-liquid flow passage (44) being arranged at the top of the reaction chamber (43)
A desiccant storage location (46), wherein the desiccant storage location (46) is arranged on the second side of the interior of the reaction tube (4), and when the rotary piston (3) is communicated with the first gas-liquid flow hole (24), the top of the desiccant storage location (46) is communicated with the bottom of the second gas-liquid flow hole (24);
a filter membrane placement position (45), wherein the filter membrane placement position (45) is arranged at the bottom of the desiccant storage position (46);
a discharge filter membrane (451), the discharge filter membrane (451) being fixedly arranged inside the filter membrane placement site (45);
the locking hook clamping position (47), the locking hook clamping position (47) is arranged in the middle of the inner side of the reaction tube (4), and the locking hook clamping position (47) is matched with the locking hook (27);
and two groups of films (48), wherein the two groups of films (48) are fixedly arranged on the bottom proximal side wall and the bottom distal side wall of the reaction tube (4) respectively.
7. The nucleic acid detection kit of claim 6, wherein the cartridge inlet gas-liquid flow channel (42) comprises:
the triangular bosses (421) are uniformly arranged on the inner wall of the bin inlet gas-liquid flow channel (42).
8. The nucleic acid detection kit according to claim 6, wherein the reaction cartridge (43) comprises:
two upper edges fixedly arranged on the upper side of the reaction bin (43);
the right-angle sawtooth structures (431) are uniformly arranged on the inner walls of the two upper edges of the reaction bin (43);
and two lower edges (432), wherein the two lower edges (432) are fixedly arranged at the lower side of the reaction bin (43).
9. The nucleic acid detecting kit according to claim 6, wherein the top end of the off-gas-liquid flow path (44) is in communication with the bottom of the filter membrane placement site (45).
10. A method for detecting nucleic acid for non-diagnostic purposes using the nucleic acid detection kit according to any one of claims 1 to 9, characterized in that the steps comprise:
s1, opening a flip cover (1), injecting a nucleic acid sample and a sample treatment liquid from a sample injection hole (11), closing the flip cover (1), and rotating a rotary wrench (33) to be parallel to a horizontal plane, wherein a gas-liquid flow hole (24) is in a communication state;
s2, the processed sample liquid passes through a filtering membrane (222), enters a gas-liquid flow through hole (24), passes through a first connecting hole (321) in the middle of a rotary piston (3), enters a freeze-dried ball storage position (41) of a reaction tube (4), and re-dissolves preset freeze-dried balls (411);
s3, the sample solution enters and fills the reaction bin (43) through a bin inlet gas-liquid flow channel (42) of the reaction tube (4), gas discharged by extrusion in the liquid flow process passes through a bin outlet gas-liquid flow channel (44) and a bin outlet filtering membrane (451) under the negative pressure effect of the negative pressure bin (23), and enters the negative pressure bin (23) through a second connecting hole (322) in the middle of the rotary piston (3);
s4, rotating the rotating wrench (33) to a vertical position, wherein the gas-liquid communication hole (24) is in a sealed state, maintaining the air pressure balance, and inserting the kit into a detection instrument for nucleic acid detection.
CN202310199122.7A 2023-03-03 2023-03-03 Nucleic acid detection kit and detection method thereof Pending CN116286312A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310199122.7A CN116286312A (en) 2023-03-03 2023-03-03 Nucleic acid detection kit and detection method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310199122.7A CN116286312A (en) 2023-03-03 2023-03-03 Nucleic acid detection kit and detection method thereof

Publications (1)

Publication Number Publication Date
CN116286312A true CN116286312A (en) 2023-06-23

Family

ID=86833550

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310199122.7A Pending CN116286312A (en) 2023-03-03 2023-03-03 Nucleic acid detection kit and detection method thereof

Country Status (1)

Country Link
CN (1) CN116286312A (en)

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