CN116267970B - Application of compound in preventing and controlling pine wood nematodes - Google Patents
Application of compound in preventing and controlling pine wood nematodes Download PDFInfo
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- CN116267970B CN116267970B CN202310335256.7A CN202310335256A CN116267970B CN 116267970 B CN116267970 B CN 116267970B CN 202310335256 A CN202310335256 A CN 202310335256A CN 116267970 B CN116267970 B CN 116267970B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/08—Amines; Quaternary ammonium compounds containing oxygen or sulfur
- A01N33/10—Amines; Quaternary ammonium compounds containing oxygen or sulfur having at least one oxygen or sulfur atom directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application discloses application of a compound in preventing and controlling pine wood nematodes. The application adopts BxSCPI compound to prevent and treat pine wood nematodes; proved by experiments, the compound ID is 6574212, 7990798, 6433663 and 7655389, has a certain inhibition effect on nematode growth and good insecticidal activity; becomes a potential medicine for preventing and treating pine wood nematodes and provides a new control means for preventing and treating pine wood nematodes.
Description
The application relates to a divisional application, the application number of the original application is 2022110170893, the application date is 2022, 8 months and 23 days, and the name of the application is the application of a compound in preventing and controlling pine wood nematodes.
Technical Field
The application belongs to the technical field of agriculture, and particularly relates to application of a compound in preventing and controlling pine wood nematodes.
Background
Pine wood nematode (Bursaphelenchus xylophilus) disease is a destructive pine tree disease, is rapidly transmitted, causes huge damage and serious economic loss to the pine forest ecological system, and is a serious quarantine disease at present. At present, the chemical prevention and treatment cost is high, the threat to the ecological environment of pine and the health of people and animals is not consistent with the green prevention and control concept advocated by the nation, and the efficient biological prevention and treatment preparation and application means become research hot spots. Therefore, the development of novel pine wood nematode pesticides is of great importance.
Disclosure of Invention
The application aims to provide a compound capable of effectively preventing and controlling pine wood nematodes.
The technical scheme adopted by the application is as follows:
in a first aspect of the application, there is provided the use of a compound or salt thereof for controlling pine wood nematodes, the compound having the structural formula:
in a second aspect of the application there is provided the use of a compound or salt thereof in the manufacture of a product for controlling pine wood nematodes, the compound being as described in the first aspect of the application.
In some embodiments of the application, the product is a pharmaceutical.
In some embodiments of the application, the medicament further comprises a pharmaceutically acceptable excipient.
In some embodiments of the application, the medicament is formulated into any pharmaceutically acceptable dosage form.
In some embodiments of the application, the medicament is formulated as a dry powder, wettable powder, emulsifiable concentrate, microemulsion, paste, granule, solution or suspension.
In a third aspect of the application there is provided a pharmaceutical composition for controlling pine wood nematodes comprising a compound according to the first aspect of the application or a salt thereof.
In some embodiments of the application, the pharmaceutical composition further comprises emamectin benzoate and/or avermectin other components for controlling pine wood nematodes.
In some embodiments of the application, the pharmaceutical composition further comprises a pharmaceutically acceptable excipient.
In some embodiments of the application, the pharmaceutical composition is formulated into any one of the pharmaceutically acceptable dosage forms.
In some embodiments of the application, the pharmaceutical composition is formulated as a dry powder, wettable powder, emulsifiable concentrate, microemulsion, paste, granule, solution or suspension.
In a fourth aspect the present application provides a method of controlling pests, comprising applying a pharmaceutical composition according to the third aspect of the present application to pine wood nematodes and/or pine wood nematode habitat, using a compound according to the first aspect of the present application or a salt thereof.
In some embodiments of the application, the concentration of the compound is from 10 to 10 5 μM。
The beneficial effects of the application are as follows:
the absence of cholesterol in the nematode will result in a decrease in the number of eggs of the next generation of nematodes. When cholesterol is added again, the fertility of the nematodes can be recovered. The nematode sperm plasma membrane has very rich cholesterol content, and the cholesterol and sphingomyelin constitute lipid which is favorable for the activation of sperm. Cholesterol can affect the composition of sperm lipids and thus hinder sperm activation. Cholesterol plays an important role in the formation and activity of the nematode sperm, as well as the number of offspring. Therefore, based on pine wood nematode sterol transporter (Sterol Carrier Protein, SCP 2) as a target, a friendly and effective inhibitor BxSCPI (SCP-2 inhibitor, SCPI) is designed and screened, and the accumulation and utilization of intracellular cholesterol are influenced by competing with cholesterol to bind with BxSCP-2, so that the growth and reproduction of nematodes are influenced. The BxSCPI compound is used for preventing and controlling the pine wood nematodes, and provides a new technology and a new thought for biological prevention and control of the pine wood nematodes.
The application adopts BxSCPI compound to prevent and treat pine wood nematodes; proved by experiments, the compound ID is 6574212, 7990798, 6433663 and 7655389, has a certain inhibition effect on nematode growth and good insecticidal activity; becomes a potential medicine for preventing and treating pine wood nematodes and provides a new control means for preventing and treating pine wood nematodes.
Drawings
FIG. 1 survival of nematodes 120h after drug inoculation.
Figure 24 inhibition of pine wood nematode growth by compounds.
Figure 3 4 survival of pine wood nematodes after 96h of drug treatment.
Detailed Description
The conception and the technical effects produced by the present application will be clearly and completely described in conjunction with the embodiments below to fully understand the objects, features and effects of the present application. It is apparent that the described embodiments are only some embodiments of the present application, but not all embodiments, and that other embodiments obtained by those skilled in the art without inventive effort are within the scope of the present application based on the embodiments of the present application.
Example 1
1.1.1 Experimental materials
1) Test pine wood nematodes and botrytis cinerea strains: is provided by plant protection of Shanxi national institute.
2) Test compound: purchased from MCE company compound library and cambridge small molecule compound library, usa.
1.1.2 research methods
1) Nematode culture collection
Reference Huang Xiaojuan et al (2014) when Botrytis cinerea is cultivated on PDA culture medium for 5-6 days and fully grows on 9cm dish, pine wood nematodes are inoculated, and the pine wood nematodes are placed in a constant temperature incubator at 27 ℃ for cultivation for 7 days, and when the nematodes all enter a tolerance state period. The nematode liquid is eluted by sterile water and collected in a 15ml centrifuge tube, and centrifuged at 4000r/min for 1min, and the supernatant is removed. Washing with sterile water for 2-3 times, and diluting to prepare the nematode suspension with the solubility of 2000 strips/mL.
2) Preparation of the Compounds
Referring to the study method of day et al (2019), 10 compounds were dissolved separately using DMSO. First, each drug concentration is prepared into mother liquor 3×10 4 Mu M, then diluted to 1X 10 sequentially with sterile water 4 μM、1×10 3 mu.M, 300. Mu.M, 100. Mu.M and 50. Mu.M.
The information about 10 compounds is shown in Table 1.
TABLE 1
3) Determination of influence and lethal effect of medicine on growth of pine wood nematodes
a. In an ultra clean bench, 20mL of the preparation was added to a sterile disposable culture dish with a diameter of 9cmThe PDA culture medium is cooled and solidified, a puncher with the diameter of 6mm is adopted to inoculate a cultured Botrytis cinerea colony at the center of each plate, and the culture is carried out for 5 days at the temperature of 27 ℃ until hyphae are fully distributed on the plate. The mycelium surface was uniformly sprayed with 100. Mu.M and 1X 10 concentrations, respectively, using a 1 mL-volume micro-spray bottle 3 μM、1×10 4 Mu M drug solution was sprayed uniformly with 0.5mL of each dish. After standing for 10min, 0.5mL of nematode solution (1000) is inoculated in each dish, an equal volume of DMSO solution with the concentration of the same dilution multiple is used as a control, 3 dishes are arranged in each treatment, and an equal volume of DMSO with the same concentration is used as a control. Sealing the culture dish with a Parafilm sealing film, placing the culture dish in a 26 ℃ incubator for dark culture, and observing and counting the death number and the growth condition of nematodes under an optical microscope every 24 hours. As a result, as shown in FIG. 1, the concentration of the inoculating compound was 1X 10 4 Mu M, compound IDs 6574212, 7990798, 6433663 and 7655389 have a certain inhibition effect on nematode growth. Compared with the control, the nematode is observed under a microscope after 120 hours, the nematodes treated by the 4 drugs are all dead, and the nematode carcasses are digested. And the growth vigor of the nematodes after the other drugs are treated is the same as that of the control DMSO, the nematodes are propagated in a large quantity, and the dish cover is filled with nematodes of different ages.
b. The 4 compounds screened were further validated using sterile 24-well cell culture plates. 100. Mu.L of the above prepared nematode suspension was added to each well, and then 100. Mu.L of each of the drug solutions at a concentration of 100. Mu.M and 50. Mu.M in this order was added, respectively, with the DMSO solution at the same dilution concentration as the control. Each concentration was set with 6 replicates of 1 well (about 200 nematodes). The culture plates are sealed by Parafilm sealing films and then placed in a 26 ℃ incubator for dark culture, the death number and the survival number of nematodes are observed and counted under an optical microscope every 12 hours, and the mortality rate is calculated. As shown in figures 2 and 3, the nematodes die in a large amount after 96 hours of drug treatment, and the final nematode survival rate is lower than 10%, so that the nematoda prevention and treatment effect is very good.
The present application has been described in detail in the above embodiments, but the present application is not limited to the above examples, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present application. Furthermore, embodiments of the application and features of the embodiments may be combined with each other without conflict.
Claims (7)
1. The application of the compound or the salt thereof in preventing and controlling pine wood nematodes is characterized in that the structural formula of the compound is shown as follows:
。
2. the application of the compound or the salt thereof in preparing products for preventing and controlling pine wood nematodes is characterized in that the structural formula of the compound is shown as follows:
。
3. the use according to claim 2, wherein the product is a pesticide.
4. The use according to claim 3, wherein the pesticide further comprises a pesticidally acceptable adjuvant.
5. The use according to claim 3, wherein the pesticide is formulated into any pesticidally acceptable dosage form.
6. A method of controlling pine wood nematodes comprising applying to pine wood nematodes or to pine wood nematode habitat a compound as claimed in claim 1 or claim 2 or a salt thereof.
7. The method according to claim 6, wherein the concentration of the compound is 10 to 10 5 μM。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310335256.7A CN116267970B (en) | 2022-08-23 | 2022-08-23 | Application of compound in preventing and controlling pine wood nematodes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310335256.7A CN116267970B (en) | 2022-08-23 | 2022-08-23 | Application of compound in preventing and controlling pine wood nematodes |
| CN202211017089.3A CN115517257B (en) | 2022-08-23 | 2022-08-23 | Application of compound in preventing and controlling pine wood nematodes |
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| CN202211017089.3A Division CN115517257B (en) | 2022-08-23 | 2022-08-23 | Application of compound in preventing and controlling pine wood nematodes |
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| CN116267970A CN116267970A (en) | 2023-06-23 |
| CN116267970B true CN116267970B (en) | 2023-10-20 |
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| CN202211017089.3A Active CN115517257B (en) | 2022-08-23 | 2022-08-23 | Application of compound in preventing and controlling pine wood nematodes |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61122201A (en) * | 1984-11-20 | 1986-06-10 | Nippon Tokushu Noyaku Seizo Kk | Agent for compatting bursaphelencus lignocolus and method of preventing pine trees from withered |
| JPH11349414A (en) * | 1998-06-08 | 1999-12-21 | Ube Ind Ltd | Controlling agent for bursaphelenchus lignicolus |
| CN1907930A (en) * | 2006-08-11 | 2007-02-07 | 云南大学 | Compound for poisoning pine wood nematode and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100399896C (en) * | 2005-05-10 | 2008-07-09 | 赵昕 | Application of alpha-trithiophene in control of pine wood nematode disease |
| CN114557359A (en) * | 2022-01-14 | 2022-05-31 | 杭州益森键生物科技有限公司 | Pesticide composition for preventing and treating pine wood nematode disease and compound preparation and application thereof |
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2022
- 2022-08-23 CN CN202310335256.7A patent/CN116267970B/en active Active
- 2022-08-23 CN CN202211017089.3A patent/CN115517257B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61122201A (en) * | 1984-11-20 | 1986-06-10 | Nippon Tokushu Noyaku Seizo Kk | Agent for compatting bursaphelencus lignocolus and method of preventing pine trees from withered |
| JPH11349414A (en) * | 1998-06-08 | 1999-12-21 | Ube Ind Ltd | Controlling agent for bursaphelenchus lignicolus |
| CN1907930A (en) * | 2006-08-11 | 2007-02-07 | 云南大学 | Compound for poisoning pine wood nematode and application thereof |
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| Publication number | Publication date |
|---|---|
| CN115517257A (en) | 2022-12-27 |
| CN116267970A (en) | 2023-06-23 |
| CN115517257B (en) | 2023-08-01 |
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