CN116265577A - Plasma mutation breeding device - Google Patents

Plasma mutation breeding device Download PDF

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Publication number
CN116265577A
CN116265577A CN202111549420.1A CN202111549420A CN116265577A CN 116265577 A CN116265577 A CN 116265577A CN 202111549420 A CN202111549420 A CN 202111549420A CN 116265577 A CN116265577 A CN 116265577A
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plasma
mutation breeding
clean working
working bin
breeding device
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王立言
沈小娟
周震
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Wuxi Tmaxtree Biotechnology Co ltd
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Wuxi Tmaxtree Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract

The invention relates to a plasma mutation breeding device which comprises a sample processing system, a cooling system, a detection system and a control system, wherein the control of the working environment of a clean working bin is realized based on the control system and set parameters. The invention can effectively control the mutation environment, improve the biological activity of different samples of plants and animals under the condition of mutation treatment, and meet the mutation breeding requirements of different samples.

Description

Plasma mutation breeding device
Technical Field
The invention belongs to the field of plasma technology treatment equipment, and particularly relates to a plasma mutation breeding device.
Background
The breeding of high-quality germplasm resources is one of the core works of the modern biological industry.
As a new mutation breeding technology, the plasma technology not only overcomes the defects of long period, slow progress and difficult obtaining of high-efficiency variant varieties in conventional breeding, but also has more efficiency and cost advantages than genetic engineering, and particularly has more unique advantages for biological cells with complex metabolic networks and non-GMO mutation methods. The plasma is a fourth state of a substance which coexists with the solid state, the liquid state and the gas state of the substance, and is a substance form which is composed mainly of a large number of free electrons and charged ions. Research shows that a large amount of active particles in the plasma can act on different biological samples, and cause improvement of various target properties, and has good application prospect in the field of biotechnology.
Currently, plasma technology has been applied to biological mutation breeding, and various mutation breeding devices have been prepared, such as: patent document 1 (publication number CN 202297604U) discloses a plasma microorganism mutation breeding device, which uses plasma jet generated under the conditions of atmospheric pressure and low voltage to carry out mutation breeding on biological samples, has high uniformity and low temperature, and avoids the phenomenon that the jet temperature is too high to cause death of a large number of microorganism samples; patent document 2 (publication No. CN 203700376U) discloses a normal pressure room temperature plasma mutation breeding device, which adopts a cooling system to cool a plasma generator, and is equipped with a multifunctional control operation panel to realize automatic monitoring and control functions, so that plasma mutation breeding under normal pressure room temperature environment is completed more efficiently; patent document 3 (publication No. CN 207653229U) discloses a plasma plant mutagenesis apparatus that provides a cold plasma plant mutagenesis apparatus that can generate stable high-energy ion energy for plant mutagenesis at atmospheric pressure without a vacuum environment. Although the above patent documents all relate to plasma mutagenesis apparatuses which are all of an open structure, have no special requirement for a sterile environment, and are applicable to microorganisms, plant seeds, and the like in suspension, the use of animal cells, plant calli, and the like, which require a strict sterile environment, is limited; in view of the above problems, patent document 4 (CN 103911279B) and patent document 5 (CN 103981091 a) disclose a device and method for acting on biological materials by plasma and a novel atmospheric pressure room temperature plasma mutagenesis breeding device, respectively, which perform plasma mutagenesis by performing plasma mutagenesis in a clean working chamber without bioactive contaminants, and at least one sterilizing device installation position is reserved on the inner cavity or wall of the clean working chamber; ensures the high-efficiency and quick treatment of the plasmas, can automatically disinfect, and ensures that the mutagenesis process is not polluted by mixed bacteria. However, the clean working bin in the above patent document adopts a sterilizing device to realize aseptic operation, and long-time plasma jet treatment on sample materials can cause surface drying, especially the aseptic air flow provided with an air inlet and an air outlet in the clean working bin can accelerate the drying of the sample materials, thereby influencing the treatment effect and the survival ability; the operation treatment space is small, the single sterile treatment capacity is limited, and the requirements of mutagenizing different samples of plants and animals in a sterile environment cannot be met.
Disclosure of Invention
The invention provides a plasma mutation breeding device aiming at the problems in the prior art, which can effectively preserve moisture, can improve the activity after treatment in the mutation treatment process of different samples of plants and animals, has large operation space and large single treatment capacity, and can meet the requirements of the mutation treatment of different samples of sterile plants and animals.
The technical scheme of the invention is as follows:
the plasma mutation breeding device comprises a sample processing system, a cooling system, a detection system and a control system, wherein the sample processing system comprises a clean working bin without bioactive pollutants, a plasma generator, a radio frequency power supply module connected with the plasma generator, a carrier module below the plasma generator and a humidifying module; the cooling system is used for cooling the plasma generator; the detection system comprises a gas flow controller for detecting the flow of working gas for generating plasma jet, a temperature sensor for detecting the temperature for generating plasma jet, a humidity sensor for detecting the humidity in a clean working bin and a position sensor for positioning a carrying module; the control system comprises an operation panel and a controller, wherein the operation panel and the controller are used for controlling the operation of the mutation breeding device, the controller is respectively connected with the radio frequency power supply module, the object carrying module, the gas flow controller, the temperature sensor, the humidity sensor, the cooling system and the operation panel, and the control of the working environment of the clean working bin is realized based on set parameters.
Preferably, the control system controls the humidity in the clean working bin to be 55-95% through controlling the humidity sensor and the humidifying module; further preferably 65 to 85%.
Preferably, the humidifying module is a micro-spraying device.
Further preferably, the atomizing nozzle of the miniature spraying device is arranged on the inner wall of the clean working bin or hung on the top surface of the clean working bin.
Further preferably, the spraying of the micro-spraying device is sterile water and/or 75% ethanol.
Preferably, the clean working bin is connected with the sterile air device, and a lighting device and/or a disinfection and sterilization device are arranged in the clean working bin.
Further preferably, the sterile air guide port of the sterile air device is arranged on the back surface of the clean working bin body, and the disinfection and sterilization device is arranged on the side wall of the clean working bin.
Preferably, the carrying module is a movable workbench, and the movable workbench moves in a vertical lifting and/or horizontal sliding manner.
Preferably, the vertical lifting is vertical rotation lifting, and the horizontal sliding is horizontal back and forth sliding through a guide rail.
Further preferably, a position sensor is provided on the movable table.
Preferably, the movable worktable is provided with a movable stage which accommodates 6000-10000 mm 2 Area capacity of biological sample.
Further preferably, the emission surface area of the corresponding plasma generator above the movable workbench is 6000-10000 mm 2 Is a plasma of (a). The plasma isThe average temperature of the body is 40 ℃ or lower.
The invention has the following technical effects:
according to the plasma mutation breeding device, the humidifying module is arranged on the wall of the clean working bin, and the humidifying module is controlled to perform spraying operation through the controller or the parameter setting of the controller based on the humidity sensor, so that the air humidity of the clean working bin is maintained, the surface humidity of biological materials can be maintained under the working environment of the ion jet or the sterile wind and the ion jet, and the influence of the activity of the biological materials caused by the humidity problem is avoided.
The invention is provided with the movable workbench capable of vertically lifting and/or horizontally sliding, and the biological materials on the carrying module can be immediately far away from the plasma generator and/or the sterile air outlet after being processed by the vertical lifting and/or the horizontal sliding, so that the action intensity and the action time of air flow on the materials are reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of an embodiment of a plasma mutation breeding device of the invention;
FIG. 2 is a perspective view of an embodiment of a plasma mutation breeding device of the present invention;
FIG. 3 is a perspective view of another embodiment of the plasma mutation breeding device of the present invention.
1. A sample processing system; 2. a cooling system; 3. a detection system; 4. a control system; 5. a clean working bin; 6. a plasma generator; 7. a radio frequency power supply module; 8. a plasma jet port; 9. a carrying module; 10. a humidification module; 11. a gas flow controller; 12. a temperature sensor; 13. a humidity sensor; 14. a position sensor; 15 an operation panel; a 16 controller; 17 a disinfection and sterilization device; 18. a lighting device; 19. an operation room door; 20. a sterile air guide port; 21. an air inlet window; 22. a heat dissipation window; 23. a power interface; 24. a stepping motor; 25. a housing.
Detailed Description
Specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While specific embodiments of the invention are shown in the drawings, it should be understood that the invention may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
It should be noted that certain terms are used throughout the description and claims to refer to particular components. Those of skill in the art will understand that a person may refer to the same component by different names. The description and claims do not identify differences in terms of components, but rather differences in terms of the functionality of the components. As used throughout the specification and claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. The description hereinafter sets forth a preferred embodiment for practicing the invention, but is not intended to limit the scope of the invention, as the description proceeds with reference to the general principles of the description. The scope of the invention is defined by the appended claims.
For the purpose of facilitating an understanding of the embodiments of the invention, reference will now be made to the drawings of several embodiments illustrated in the drawings, and the accompanying drawings are not to be taken as limiting the embodiments of the invention.
Specifically, as shown in FIG. 1, the plasma mutation breeding device of the present invention uses plasma technology to mutagenize biological materials that need to be strictly sterilized at normal pressure and room temperature. The plasma mutation breeding device comprises a sample processing system (1), a cooling system (2), a detection system (3) and a control system (4).
The sample processing system (1) comprises a clean working bin (5) without bioactive pollutants, a plasma generator (6), a radio frequency power supply module (7) connected with the plasma generator, a carrying module (9) below the plasma generator and a humidifying module (10).
The cooling system (2) is used for cooling the plasma generator.
The detection system (3) comprises a gas flow controller (11) for detecting the flow of working gas for generating plasma jet, a temperature sensor (12) for detecting the temperature for generating plasma jet, a humidity sensor (13) for detecting the humidity in a clean working bin and a position sensor (14) for positioning a carrying module.
The control system (4) comprises an operation panel (15) and a controller (16) for controlling the operation of the mutation breeding device, wherein the controller (16) is respectively connected with the radio frequency power module (7), the object carrying module (9), the gas flow controller (11), the temperature sensor (12), the humidity sensor (13), the position sensor (14), the cooling system (2) and the operation panel (15), and the control of the working environment of the clean working bin (5) is realized based on set parameters.
The plasma generator (6) is connected with the radio frequency power supply module (7), the working gas pipeline and the cooling system (2) to generate normal-pressure room-temperature plasma for mutation breeding. The working gas pipeline is a transmission pipeline of working gas, namely a pipeline connected with a plasma generator by a gas cylinder filled with gas for exciting and generating plasma.
One end of a jet port of the plasma generator (6), namely a plasma jet port (8), a carrying module (9) and a humidifying module (10), is arranged in the clean working bin (5); further, the plasma generator (6) is arranged at the top end of the clean working bin (5), the object carrying module (9) is arranged in the clean working bin (5) by taking the bottom surface of the clean working bin (5) as a support, and the humidifying module (10) is arranged at a mounting position reserved in the inner cavity or the wall of the clean working bin (5).
The cooling system (2) is a water cooling system or an air cooling system and further comprises a refrigeration water pump, a water tank or a refrigeration wind system; the refrigerating water pump, the water tank or the refrigerating wind system is connected with the plasma generator through a pipeline. In one embodiment, the cooling system comprises a cooling water circulator connected to the plasma generator to form internal circulation refrigeration.
In one embodiment, a gas flow controller (11) is connected with the plasma generator (6) and feeds back gas flow information to a controller (16) to control the flow of the working gas of the plasma generator on line in real time. The temperature sensor (12) is used for detecting the temperature of the cooling system and feeding back temperature information to the controller (16) for temperature centralized control and real-time monitoring. The humidity sensor (13) is arranged on the inner cavity or the wall of the clean working bin (5) and is used for detecting the humidity in the clean working bin (5) and feeding back humidity information to the controller (15) so that the controller can realize on-line control of the humidity through the humidifying module (10).
In one embodiment, the operating panel (15) sets processing parameters such as gas flow, radio frequency power supply, cooling system temperature, plasma processing time, humidity in the clean room, etc., and the controller controls the emission of the plasma generator (6) and its processing of the biological sample based on the processing parameter settings and signals from the gas flow controller (11), radio frequency power supply module (7) power sensor, temperature sensor (12), humidity sensor (13), etc.
As shown in fig. 2 and 3, in one embodiment of the plasma mutation breeding device of the present invention, the working environment of the clean working chamber (5) is ensured by a sterile wind device and a sterilization device.
In one embodiment, the clean working bin (5) is connected with a sterile wind device, and the sterile wind system at least comprises a fan for generating sterile wind, a filter element and a pumping unit. Besides the operation room door (19), the other three surfaces of the clean operation room (5) are provided with the baffle plates which are connected with each other, and the baffle plates above the clean operation room (5) or the baffle plates of the back of the body, which are opposite to the operation table, are provided with the sterile air guide ports (20), so that the sterile air enters the clean operation room (5) through the sterile air guide ports (20).
In one embodiment, the operation room door (19) is a door which is opened and closed by pushing and pulling up and down, and the operation room door (19) comprises a window which is provided with a transparent and convenient for observation.
In one embodiment, the clean working cabin (5) is internally provided with a lighting device (18) and/or a disinfection and sterilization device (17). The disinfection and sterilization device (17) is any one of an ultraviolet disinfection device and a chemical disinfection device. In a specific embodiment, the disinfection and sterilization devices (17) are ultraviolet lamps, more than 2 disinfection and sterilization devices are respectively arranged on the inner side walls of the clean working bin (5). The lighting device (18) may be a lighting device that provides a good lighting function. The lighting device and/or the disinfection and sterilization device can realize the control of the switch and the time on the operation panel (15) through the controller (16). In the operation process, the clean working bin (5) is sterilized in advance, ventilation is carried out after sterilization is finished, and then biological materials are put into the clean working bin for plasma irradiation treatment. The cleaning grade in the cleaning work bin (5) reaches 10 ten thousand grades.
In one embodiment, the carrying module (9) is arranged in the clean working bin (5), and the upper end of the carrying module (9) is a platform for placing biological samples. As shown in fig. 2, the carrying module (9) is a vertically lifting movable workbench, and the upper end of the vertically lifting movable workbench is a carrying tray which accommodates 6000-10000 mm 2 An area capacity of biological sample; the emission surface area of the corresponding plasma generator above the movable workbench is 6000-10000 mm 2 Is a plasma of (a); the average temperature of the plasma is 40 ℃ or lower. The carrying disc is supported by the shaft rod, a stepping motor capable of accurately controlling vertical lifting is arranged below the clean working bin (5), and the carrying disc further comprises a motor capable of controlling axial rotation. When the object carrying module (9) is a vertically lifting movable workbench, the shape of the jet nozzle of the plasma generator is circular, so that the highest utilization rate of the plasma jet is ensured.
In one embodiment, the carrying module (9) is arranged in the clean working bin (5), and the upper end of the carrying module (9) is a platform for placing biological samples. As shown in FIG. 3, the carrying module (9) is a horizontal sliding movable workbench, and a movable object stage is arranged on the horizontal sliding movable workbench and is used for accommodating 6000-8000 mm 2 An area capacity of biological sample; the emission surface area of the corresponding plasma generator above the movable workbench is 6000-8000 mm 2 Is a plasma of (a); the average temperature of the plasma is 40 ℃ or lower. The horizontal sliding movable workbench horizontally slides back and forth through a stepping motor (24). When the carrying module (9) is a horizontally sliding movable workbench, the plasma generator jet flow ensures the highest utilization rate of the plasma jet flow.
In the foregoing embodiments, the structure of the plasma mutation breeding device shown in fig. 2 and 3 is the same as the carrier module except that the names of the components are not identified in fig. 3.
In one embodiment, the vertical lift movable stage and the horizontal slide movable stage are each controllable by a controller (16) to position the load tray or stage by a stepper motor (24) based on a position sensor (14) signal.
Because biological materials such as fish eggs, plant callus and the like are easier to take away water on the surface of the biological materials during the plasma irradiation treatment process, the mutagenesis effect is affected, and even the biological sample materials are inactivated. In order to solve the problem, the humidity sensor (13) and the humidifying module (10) are arranged in the clean working bin (5), and humidity parameters in the clean working bin (5) are fed back to the controller (16) through the humidity sensor (13) to realize humidity control in the clean working bin (5).
In a specific embodiment, the humidifying module (10) is a miniature spraying device, and comprises an atomizer, a liquid adding pipeline and a control unit. The liquid adding pipeline can be one or more, and sterile water and/or 75% ethanol are added. When 75% ethanol is present in the liquid pipeline, spray sterilization is preferably performed before use, then sterilization by ultraviolet and other modes is performed, then the humidity in the clean working bin (5) is detected according to a humidity sensor, and sterile water spray and spray quantity thereof are selected according to the situation.
The number of atomizers is not limited and may be one or more. Further, in the case of one atomizer, a plurality of atomizing nozzles may be provided. In a specific embodiment, the atomizing nozzle is an ultrasonic atomizing nozzle for atomizing water into mist by ultrasonic waves. The atomizing time and the water mist generation amount are automatically controlled by a control unit and a controller (16). In one embodiment, the atomizing nozzle of the miniature spraying device is arranged on the inner wall of the clean working cabin or hung on the cabin top surface of the clean working cabin (5).
The humidifying module (10) sprays water mist in the clean working bin (5) to enable the water mist to be ultra-fine particles to float in the clean working bin (5), so that the air humidity is improved. The control system controls the humidity in the clean working bin to be 55-95% through controlling the humidity sensor and the humidifying module; further preferably 65 to 85%.
In one embodiment, the plasma mutation breeding device comprises a shell (25), wherein the left side of the shell is provided with a clean working bin (5), and the right side of the shell is provided with an operation panel (15). An air inlet window (21) of the sterile air device is arranged on a shell (25) above the clean working bin (5), a fan is connected with the clean working bin (5) through an air duct, and air entering from the air inlet window (21) is processed through a filter and blown in from the top or the back of the sterile working bin (5). The operation panel (15) is arranged on the outer surface of the shell (25), and the power interface (23) is arranged on the outer side wall of the shell (25), and more preferably on the left outer side wall. A heat dissipation window (22) is arranged on the right outer side wall of the shell (25), so that heat dissipation of the radio frequency power supply module (7), the controller (16), the humidifying module (10) and the like is facilitated.
The plasma mutation breeding device is provided with the clean working bin, so that the aseptic environment is ensured, and the operation treatment of materials requiring the aseptic environment is facilitated; the humidifying module is arranged on the wall of the clean working bin and is set based on parameters of the humidity sensor through the controller or the controller, so that the humidifying module is effectively controlled, the air humidity of the clean working bin is effectively ensured, the surface humidity of biological materials is ensured even under the environment of sterile wind and ion jet flow, the activity of the biological materials is favorably maintained, and the influence of experimental environments caused by air flow is reduced.
In the invention, the movable workbench capable of vertically lifting and/or horizontally sliding is also arranged, so that after biological materials on the carrying module are processed by vertical lifting and/or horizontal sliding, the biological materials are immediately far away from the plasma generator and/or the sterile air outlet, and the action intensity and action time of air flow on the materials are reduced.
Examples
The plasma mutation breeding apparatus of the present invention will be further described with reference to examples.
The plasma mutation breeding device shown in fig. 2 is adopted to treat the fertilized eggs of the crucian at different humidity, the humidity is set to be 45%, 55%, 65%, 75%, 85% and 95% to treat the fertilized eggs of the crucian respectively, and the result shows that when the humidity reaches 95%, the clean working bin wall has small water drops, but the plasma generator works normally to generate plasma. The humidity is 45 percent, and the fertilized egg surface is slightly dehydrated and atrophic by plasma treatment; the surface of the fertilized egg loses luster when the humidity is 55%; the emergence rate of the hatched treatment group with the humidity of 45 percent and 55 percent is obviously lower than that of the treatment group with the humidity of 65 percent to 95 percent; the emergence rates of 85% of the treated groups and 95% of the treated groups were not very different.
Maize calli were treated with the plasma mutation breeding apparatus shown in fig. 3 at different humidity. The humidity is set to be 45%, 55%, 65%, 75%, 85% and 95% respectively for treating the corn callus, and the results show that the humidity is 45% and 55% respectively for plasma irradiation treatment, the corn callus has slight water loss, the surface loses gloss, the browning of the humidity 45% and 55% treated group is obviously higher than that of a 65-95% control group after 2 days of induction culture, and the browning rate of the callus of the 75% treated group is not greatly different from that of the callus of the 85% treated group and the 95% treated group.

Claims (10)

1. The plasma mutation breeding device is characterized by comprising a sample processing system, a cooling system, a detection system and a control system, wherein,
the sample processing system comprises a clean working bin without bioactive pollutants, a plasma generator, a radio frequency power supply module connected with the plasma generator, a carrying module below the plasma generator and a humidifying module;
the cooling system is used for cooling the plasma generator;
the detection system comprises a gas flow controller for detecting the flow of working gas for generating plasma jet, a temperature sensor for detecting the temperature for generating plasma jet, a humidity sensor for detecting the humidity in a clean working bin and a position sensor for positioning a carrying module;
the control system comprises an operation panel and a controller, wherein the operation panel and the controller are used for controlling the operation of the mutation breeding device, the controller is respectively connected with the radio frequency power supply module, the object carrying module, the gas flow controller, the temperature sensor, the humidity sensor, the cooling system and the operation panel, and the control of the working environment of the clean working bin is realized based on set parameters.
2. The plasma mutation breeding device according to claim 1, wherein the control system controls the humidity in the clean working bin to be 55-95% by controlling the humidity sensor and the humidifying module; further preferably 65 to 85%.
3. The plasma mutation breeding device of claim 1, wherein the humidification module is a micro-spray device.
4. A plasma mutagenic breeding device according to claim 3, wherein the atomizing nozzle of said micro-atomizing device is arranged on the inner wall of the clean working chamber or is suspended on the top surface of the clean working chamber.
5. A plasma mutation breeding device according to claim 3, wherein the spray of the micro spray device is sterile water and/or 75% ethanol.
6. The plasma mutation breeding device according to claim 1, wherein the clean working bin is connected with a sterile wind device, and a lighting device and/or a disinfection and sterilization device are arranged in the clean working bin.
7. The plasma mutation breeding device according to claim 6, wherein the sterile air guide opening of the sterile air device is arranged on the back surface of the clean working bin body, and the disinfection and sterilization device is arranged on the side wall of the clean working bin.
8. The plasma mutation breeding device according to claim 1, wherein the carrying module is a movable workbench, and the movable workbench moves in a vertical lifting and/or horizontal sliding manner.
9. The plasma mutation breeding apparatus according to claim 1, wherein the vertical lift is a vertical rotation lift, and the horizontal slide is a horizontal back and forth slide via a guide rail.
10. The plasma mutation breeding device of any one of claims 1, 6-9, wherein a position sensor is provided on the movable table.
CN202111549420.1A 2021-12-17 2021-12-17 Plasma mutation breeding device Pending CN116265577A (en)

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Application Number Priority Date Filing Date Title
CN202111549420.1A CN116265577A (en) 2021-12-17 2021-12-17 Plasma mutation breeding device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111549420.1A CN116265577A (en) 2021-12-17 2021-12-17 Plasma mutation breeding device

Publications (1)

Publication Number Publication Date
CN116265577A true CN116265577A (en) 2023-06-20

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Family Applications (1)

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Country Link
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