CN116265377A

CN116265377A - Device for introducing liquid into container

Info

Publication number: CN116265377A
Application number: CN202111552192.3A
Authority: CN
Inventors: 王立言; 仝银杏
Original assignee: Luoyang Tmaxtree Biotechnology Co ltd
Current assignee: Luoyang Tmaxtree Biotechnology Co ltd
Priority date: 2021-12-17
Filing date: 2021-12-17
Publication date: 2023-06-20

Abstract

The invention relates to a device for leading liquid into a container, which comprises a liquid storage container for storing sample liquid, a first pipeline connected with a mouth of the liquid storage container, and a second pipeline connected with the bottom of the liquid storage container, wherein the other end of the first pipeline is connected with a buffer bottle, a third pipeline connected with a power source is further arranged on the buffer bottle, the other end of the second pipeline is connected with the sample liquid container, and the power source enables the pipeline and the liquid storage container to form negative pressure through air suction so as to realize that the sample liquid enters the liquid storage container. The invention can effectively solve the problems of large sample injection fluctuation, easy bubble generation, easy pollution and the like caused by conventional direct sample injection, and has simple operation, time saving and labor saving.

Description

Device for introducing liquid into container

Technical Field

The invention relates to the field of microfluidic liquid guiding, in particular to a device for guiding liquid into a container.

Background

Microfluidic technology is widely applied to sample preparation, reaction, separation, detection and other works in biological, chemical, medical and other analysis processes. The introduction of liquid into the reservoir is often accomplished by manual direct loading, such as by manual injection through a syringe. However, in this way, the flow path pipeline is a micro pipeline, so that the complex sample injection operation, high resistance and easy bacteria contamination are easy to occur, and therefore, a device for introducing liquid into the container is required.

Disclosure of Invention

Aiming at the prior problems, the application provides a device which is simple to operate and can avoid the liquid introduction container of infectious microbe.

The invention aims at realizing the following technical scheme:

an apparatus for introducing a liquid into a container, comprising: the liquid storage container is used for storing sample liquid, a first pipeline connected with a liquid storage container opening, a second pipeline connected with the bottom of the liquid storage container, the other end of the first pipeline is connected with a buffer bottle, a third pipeline connected with a power source is further arranged on the buffer bottle, the other end of the second pipeline is connected with the sample liquid container, and the power source enables negative pressure to be formed in the pipeline and the liquid storage container through air suction so that the sample liquid enters the liquid storage container.

An apparatus for introducing a liquid into a container, comprising: the liquid storage container is used for storing sample liquid, the first pipeline is connected with a liquid storage container opening, the second pipeline is connected with the bottom of the liquid storage container, the other end of the first pipeline is connected with a power source, the power source is connected with a buffer bottle through a third pipeline, the other end of the second pipeline is connected with the sample liquid container, and the power source enables negative pressure to be formed in the pipeline and the liquid storage container through air suction so as to enable the sample liquid to enter the liquid storage container.

Preferably, the reservoir is made of a rigid material.

Preferably, the inner diameters of the first pipeline and the second pipeline are 10 micrometers to 5 millimeters.

Preferably, the number of the buffer bottles is 1 or more.

Preferably, the power source is a power structure for draining liquid through air pressure or hydraulic pressure; further, the power source is a pump, and any one of an air pump, a peristaltic pump, a diaphragm pump, a embolic pump and a syringe pump is more preferable.

Preferably, the flow rate of the sample liquid entering the liquid storage device is 0.01 mL/min-20 mL/min.

Preferably, the power source comprises an automatic control unit for controlling the flow rate, flow rate and time of the air flow.

Preferably, the control unit performs parameter setting through a display interface to realize automatic control.

The device overcomes the defects of complicated operation, high resistance, easy bacteria contamination and the like caused by conventional manual injection and sample addition; through setting up the buffer bottle between power supply and reservoir, can avoid the waste of sample liquid, effectively retrieve the sample liquid. The device is simple to operate, saves time and labor, and can also avoid the problem of liquid sample pollution.

The foregoing description is only an overview of the technical solutions of the present invention, to the extent that it can be implemented according to the content of the specification by those skilled in the art, and to make the above-mentioned and other objects, features and advantages of the present invention more obvious, the following description is given by way of example of the present invention.

Drawings

Various other advantages and benefits of the present invention will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. It is evident that the figures described below are only some embodiments of the invention, from which other figures can be obtained without inventive effort for a person skilled in the art.

In the drawings:

FIG. 1 is a schematic view of an embodiment of the apparatus for introducing liquid into a container of the present invention;

FIG. 2 is a schematic view of another embodiment of the liquid introducing container apparatus of the present invention.

Detailed Description

The technical solution and embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. It is evident that the technical solutions and embodiments have been described in order to facilitate the understanding of the present invention and to fully convey the scope of the invention to those skilled in the art.

In order to facilitate an understanding of the embodiments of the present invention, reference will now be made to the accompanying drawings, which are used by way of illustration of specific embodiments.

Fig. 1 and 2 show schematic views of the apparatus for introducing liquid into a container according to the present invention.

As can be seen from fig. 1, a device for introducing a liquid into a container comprises: the liquid storage container 1 for storing sample liquid is connected with a first pipeline 5 connected with a liquid storage container opening, a second pipeline 6 connected with the bottom of the liquid storage container, the other end of the first pipeline 5 is connected with a buffer bottle 2, a third pipeline 7 connected with a power source 3 is further arranged on the buffer bottle 2, the other end of the second pipeline 6 is connected with the sample liquid container 4, and the power source 3 enables the third pipeline 7, the buffer bottle 2, the first pipeline 5 and the liquid storage container 1 to form negative pressure through air suction so as to enable the sample liquid to enter the liquid storage container 1.

As can be seen from fig. 2, a device for introducing a liquid into a container comprises: the liquid storage container 1 for storing sample liquid is connected with a first pipeline 5 connected with a liquid storage container opening, a second pipeline 6 connected with the bottom of the liquid storage container, the other end of the first pipeline 5 is connected with a power source 3, the power source 3 is connected with a buffer bottle 2 through a third pipeline 7, the other end of the second pipeline 6 is connected with a sample liquid container 4, and the power source 3 enables the first pipeline 5 and the liquid storage container 1 to form negative pressure through air suction so as to enable the sample liquid to enter the liquid storage container 1.

The sample fluid is an oil phase fluid or a water-soluble sample such as a biological fluid, a protein-containing solution, a bacterial fluid, a sodium chloride solution, a microbial fluid medium, a cell suspension, and the like.

In the specific embodiment shown in fig. 1 and 2, when the power source 3 is driven, the gas in the first pipeline 5 and the liquid storage container 1 is pumped out to form negative pressure, so that the liquid in the sample liquid container 4 is driven to enter the liquid storage container 1.

In one embodiment, the reservoir 1 is made of a rigid material, which ensures that the reservoir 1 does not change flexibly during hydraulic actuation. The rigid material is a plastic material or a metal material, the plastic material is selected from any one or more of PC (polycarbonate), ABS (acrylonitrile-butadiene-styrene copolymer), PMMA (polymethyl methacrylate) and PS (polystyrene), the metal material can be a metal simple substance, a metal oxide or a metal alloy, and the metal material is selected from any one or more of iron, aluminum, copper, ferric oxide, aluminum oxide and copper oxide, and can also be an alloy formed by any one or more of iron, aluminum, copper, ferric oxide, aluminum oxide and copper oxide or an alloy formed by the metal simple substance, the metal oxide or the metal alloy and other materials.

In the invention, the inner diameters of the first pipeline 5 and the second pipeline 6 are 10 micrometers to 5 millimeters. In a specific embodiment, the inner diameters of the first pipe 5 and the second pipe 6 are the same, which is helpful for controlling the flow rate and the flow rate of the liquid in different pipes and is helpful for introducing the stability of the liquid.

In the present invention, the power source 3 is connected to the buffer bottle 2 through a third pipe 7. The power source 3 is a power source for draining liquid by air pressure.

In a specific embodiment, as shown in fig. 1, the power source 3 is a pump, the buffer bottle 2 is a closed container, and the third pipeline 7 between the buffer bottle 2 and the air pump 3 is used for sucking the gas in the buffer bottle 2. When the pump 3 is turned on, the sample solution in the sample solution container 4 is driven by the negative pressure into the liquid storage container 1, and the pump controls the flow rate and flow rate of the second solution into the liquid storage container 1 by controlling the flow rate, flow rate and time of the gas. The structure of the sample liquid container 4 containing the sample liquid is not limited, and is preferably an open structure, and the liquid can be added at any time.

In the specific embodiment shown in fig. 1, the entire apparatus structure is sealed except for the two ends, namely, the pump 3 and the sample liquid container 4, which are open structures for only gas exchange. The pump 3 and the sample liquid container 4 are protected from contamination by a filter membrane or a filter plug or the like.

In one embodiment, as shown in fig. 2, the pump 3 is disposed between the liquid storage container 1 and the buffer bottle 2 to provide driving force for the flow of the gas in the pipeline. The pump 3 controls the flow rate and the flow rate of the sample liquid into the liquid storage container 1 by controlling the flow rate and the flow rate of the gas. In this embodiment, the material of the liquid storage container 1, the buffer bottle 2 and the pipes is the same as that shown in fig. 1, the inner diameter of the liquid inlet and outlet connecting pipes of the liquid storage container 1 is 10 micrometers to 5 millimeters, the whole device structure system is an open structure for only gas exchange except two ends, namely the buffer bottle 2 and the container 4, and other parts are all sealed structures.

In one embodiment, the buffer bottle shown in fig. 1 and 2 is used to hold a sample liquid overflowed from the liquid storage container 1, the number of which is 1 or more.

In one embodiment, the control unit controls the liquid flow rate to be 0.01 mL/min-20 mL/min. The control unit sets parameters such as flow speed, flow rate, time and the like through a display interface, and realizes automatic control. Meanwhile, under the constant velocity of the liquid with the velocity of 0.01-mL/min-20-mL/min, the sample liquid is not easy to generate bubbles.

In one embodiment, the power source is a pump. Further preferred is any one of an air pump, peristaltic pump, diaphragm pump, embolic pump, syringe pump. According to the invention, the sample liquid is automatically introduced under the action of the pump, so that the defects of complicated operation, high resistance, easiness in forming bubbles, easiness in dying bacteria and the like caused by conventional manual injection sample adding are overcome. According to the invention, the automatic control of the liquid introducing container is realized by setting related parameters such as flow speed, flow rate, time and the like on the display interface, and the device adopted by the method is simple in structure, time-saving and labor-saving in operation, and is beneficial to batch liquid introducing operation.

While the application is susceptible to various modifications and alternative forms, specific embodiments have been shown by way of example in the drawings and have been described in detail herein. This application is not intended to be limited to the specific form disclosed. On the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the invention as defined by the appended claims and their legal equivalents.

Claims

1. A device for introducing a liquid into a container, comprising: the liquid storage container is used for storing sample liquid, a first pipeline connected with a liquid storage container opening, a second pipeline connected with the bottom of the liquid storage container, the other end of the first pipeline is connected with a buffer bottle, a third pipeline connected with a power source is further arranged on the buffer bottle, the other end of the second pipeline is connected with the sample liquid container, and the power source enables negative pressure to be formed in the pipeline and the liquid storage container through air suction so that the sample liquid enters the liquid storage container.

2. A device for introducing a liquid into a container, comprising: the liquid storage container is used for storing sample liquid, the first pipeline is connected with a liquid storage container opening, the second pipeline is connected with the bottom of the liquid storage container, the other end of the first pipeline is connected with a power source, the power source is connected with a buffer bottle through a third pipeline, the other end of the second pipeline is connected with the sample liquid container, and the power source enables negative pressure to be formed in the pipeline and the liquid storage container through air suction so as to enable the sample liquid to enter the liquid storage container.

3. A device for introducing liquids into a container as claimed in claim 1 or 2, wherein said reservoir is made of a rigid material.

4. The method of introducing a liquid into a vessel according to claim 1 or 2, wherein the first and second pipes have an inner diameter of 10 μm to 5 mm.

5. The device for introducing a liquid into a container according to claim 1 or 2, wherein the number of the buffer bottles is 1 or more.

6. The device for introducing liquid into a container according to claim 1 or 2, wherein the power source is a power structure for draining liquid by air pressure or hydraulic pressure; further preferred is one of an air pump, peristaltic pump, diaphragm pump, embolic pump, syringe pump.

7. The device for introducing a liquid into a container according to claim 1, wherein the flow rate of the sample liquid into the liquid reservoir is 0.01 mL/min to 20 mL/min.

8. The apparatus of claim 1 or 2, wherein the power source comprises an automatic control unit for controlling the flow rate, flow rate and time of the air stream.

9. The device for introducing liquid into a container as claimed in claim 8, wherein said control unit performs parameter setting through a display interface to realize automatic control.