CN116240096B - Microorganism sampling device in compressed air - Google Patents
Microorganism sampling device in compressed air Download PDFInfo
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- CN116240096B CN116240096B CN202310387418.1A CN202310387418A CN116240096B CN 116240096 B CN116240096 B CN 116240096B CN 202310387418 A CN202310387418 A CN 202310387418A CN 116240096 B CN116240096 B CN 116240096B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/18—Gas cleaning, e.g. scrubbers; Separation of different gases
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Abstract
The invention belongs to the technical field of microorganism detection, in particular to a microorganism sampling device in compressed air, which comprises a collecting bottle; one side of the collecting bottle is connected with an air inlet pipe, and the bottom of the air inlet pipe extends into the bottom of the collecting bottle; the other side of the collecting bottle is connected with an air outlet pipe; the air inlet pipe and the air outlet pipe are connected in parallel on a conveying pipeline prepared from compressed air through a valve; pure normal saline is filled in the collecting bottle; a top cover is arranged on the outer ring thread of the top of the collecting bottle; a plurality of sampling rods are uniformly arranged in the middle of the top cover, and the sampling rods penetrate through the top cover in a sliding manner; the normal saline cleans the compressed air, so that microorganisms in the compressed air remain in the normal saline; the detection staff takes out the sampling rod to carry out the detection work of microorganism, and the detection staff is convenient for carry out the detection in batches many times to the change condition of microorganism content in the compressed air of in time discovery has improved compressed air's purity degree.
Description
Technical Field
The invention belongs to the technical field of microorganism detection, and particularly relates to a microorganism sampling device in compressed air.
Background
The compressed air is air after the air compressor performs mechanical work to reduce the volume and increase the pressure. The compressed air has the advantages of clarity, transparency, convenient transportation, no special harmful performance and capability of working in a plurality of adverse environments; compressed air has become an important power source in today's society, and process air sources having various uses are widely moved in petroleum, chemical, metallurgical, electric, mechanical, light, textile, automobile manufacturing, electronic, food, medical, biochemical and other industries.
In the processes of pharmacy, biochemistry and food manufacturing and packaging, the pollution of microorganisms is not neglected, while the microorganism which pollutes compressed air has the particle size of most less than 2 mm, and the particles can easily pass through a compressor filter because of small particle size, and then are transmitted into water and oil through a pipe network to be gathered in a pipeline, so that the pollution is generated in the production and manufacturing of pharmacy, biochemistry and food; even if the compressed air is filtered during preparation, the compressed air microorganism detection method is still required to be periodically and regularly sampled and detected, the compressed air microorganism detection method mainly adopts a water absorption method, physiological saline is arranged in a sampling bottle, the sampling bottle is connected with a production conveying pipeline of the compressed air, gas enters the physiological saline of the sampling bottle, microorganisms in the gas are dissolved in the physiological saline and are discharged for culture, and colony counting is carried out.
The water absorption method is used for sampling, the physiological saline in the sampling bottle can be discharged out entirely at regular intervals to perform concentrated microorganism culture, and the microorganism sampling culture cannot be performed for a small number of times and for a plurality of time periods.
To this end, the invention provides a device for sampling microorganisms in compressed air.
Disclosure of Invention
In order to overcome the deficiencies of the prior art, at least one technical problem presented in the background art is solved.
The technical scheme adopted for solving the technical problems is as follows: the invention relates to a microorganism sampling device in compressed air, which comprises a collecting bottle; one side of the collecting bottle is connected with an air inlet pipe, and the bottom of the air inlet pipe extends into the bottom of the collecting bottle; the other side of the collecting bottle is connected with an air outlet pipe; the air inlet pipe and the air outlet pipe are connected in parallel on a conveying pipeline prepared from compressed air through a valve; pure normal saline is filled in the collecting bottle; a top cover is arranged on the outer ring thread of the top of the collecting bottle; a plurality of sampling rods are uniformly arranged in the middle of the top cover, and the sampling rods penetrate through the top cover in a sliding manner; compressed air enters the bottom of the collecting bottle through the air inlet pipe, so that the compressed air floats upwards from the bottom of the collecting bottle to form physiological saline, and is discharged back to the conveying pipeline from the air outlet pipe; the normal saline cleans the compressed air, so that microorganisms in the compressed air remain in the normal saline; the detection staff takes out the sampling rod to carry out the detection work of microorganism, and the detection staff is convenient for carry out the detection in batches many times to the change condition of microorganism content in the compressed air of in time discovery has improved compressed air's purity degree.
Preferably, the middle part of the top cover is provided with an annular chute; a turntable is rotatably arranged in the annular chute; a plurality of mounting holes are formed around the middle part of the turntable; the sampling rod is arranged in the mounting hole; the top surface of the top cover is fixedly connected with a cover plate; a motor is fixedly connected in the middle of the top surface of the cover plate, and a rotating shaft of the motor rotates to penetrate through the cover plate; the rotating shaft of the motor is fixedly connected with the middle part of the turntable; the middle part of the cover plate is surrounded by a plurality of fan-shaped grooves, and rubber plugs are slidably arranged in the fan-shaped grooves; the width of the circular ring formed by the fan-shaped grooves is larger than that of the circular ring formed by the mounting holes, and the circular ring are overlapped; the sampling rods rotate in the collecting bottle, so that the efficiency of adsorbing microorganisms in physiological saline onto the sampling rods is improved.
Preferably, a sliding sleeve is slidably arranged in the mounting hole; the top end of the sliding sleeve is fixedly connected with a gear; the diameter of the inner ring of the gear is the same as that of the inner ring of the sliding sleeve, and the gear and the sliding sleeve are communicated in a superposition manner; the sampling rod is arranged on the inner wall of the sliding sleeve in a sliding manner; an inner gear ring is fixedly connected with the outer ring of the top surface of the top cover; the outer rings of the gears are meshed with the inner rings of the inner gear ring; the gears rotate along with the rotary table and are matched with the inner gear ring meshed with the gears, so that the gears rotate to drive the sampling rod to rotate, the sampling rod rotates along with the rotary table, and the uniformity of microorganism aggregation on the surface of the sampling rod is improved.
Preferably, a plurality of fixing grooves are formed in the top of the inner ring of the gear in a surrounding mode; a fixed rod is arranged in the sliding sleeve in a sliding manner; the top outer ring of the fixed rod is fixedly connected with a plurality of protruding blocks; the convex blocks are in sliding fit with the inner walls of the fixing grooves; the bottom of the fixed rod is provided with a threaded hole; a threaded rod is fixedly connected to the top end of the sampling rod; the threaded rod is in threaded connection with the threaded hole; fixed rod and sampling rod fixed mounting to be convenient for detection personnel's installation and take out the sampling rod, improved the convenience of detection personnel's operation then.
Preferably, a round hole is formed in the top end of the fixing rod; the two sides of the top of the round hole are provided with L-shaped notches; a rotating handle is arranged in the round hole in a sliding manner; the two sides of the bottom of the rotating handle are fixedly connected with cross bars; the cross rod is in sliding fit with the L-shaped slot; the rotating handle penetrates through the fan-shaped groove; thereby being convenient for the installation of the detection personnel and taking out the sampling rod.
Preferably, the outer ring of the turntable is uniformly and circumferentially provided with a plurality of mounting grooves; rubber rollers are slidably arranged in the mounting grooves; the support of the rubber roller is in sliding connection with the inner wall of the mounting groove; the outer ring of the rubber roller is in sliding fit with the inner wall of the annular chute; a spring is arranged between one surface of the mounting groove, which is close to the center of the turntable, and the support of the rubber roller; the friction force of the rotary table during rotation is reduced, so that the stability of the sampling rod during rotation is improved.
Preferably, the outer ring of the sampling rod is sleeved with a plurality of sterile rubber sleeves; the outer ring of the sterile rubber sleeve is bonded with a plurality of activated carbon fiber strips in a surrounding manner; the surface at the sample stick is established through aseptic rubber sleeve cover, through the activated carbon fiber strip, adsorbs the microorganism in the normal saline at the surface of activated carbon fiber strip, and when the detection personnel detects, only need take off aseptic rubber sleeve and detect to the sample work of the detection personnel of being convenient for, simultaneously, the sample stick used repeatedly of being convenient for.
Preferably, a notch is formed in one side of the sterile rubber sleeve; the incision cuts the sterile rubber sleeve; a plurality of soft magnetic strips are fixedly connected to two sides of the notch; the soft magnetic strips at the two sides are mutually attracted; the soft magnetic strips on two sides are mutually attracted, the sterile rubber sleeve is fixed on the outer ring of the sampling rod in a winding manner, and when a detection person detects, the attracted soft magnetic strips are separated, so that the sterile rubber sleeve is unfolded into a plane, and the detection person can conveniently sample microorganisms.
Preferably, the outer ring of the sampling rod is uniformly provided with a plurality of annular grooves; the opening direction of the annular groove is inclined downwards from the inner ring to the outer ring; the inner wall of the annular groove is in sliding fit with the inner wall of the sterile rubber sleeve; the probability of contact with the sliding sleeve and the gear inner ring when the sterile rubber sleeve and the activated carbon fiber strip are installed and taken out is reduced, so that the probability of friction loss and external pollution of sampled microorganisms is reduced.
Preferably, the bottom end of the air inlet pipe is connected with a hollow round seat; the top surface of the hollow round seat is provided with a plurality of through grooves in a surrounding manner; the inner top surface of the hollow round seat is fixedly connected with a unidirectional diaphragm; the bottom surface of the unidirectional diaphragm is in sliding fit with the inner bottom surface of the hollow round seat; thereby improving the dispersion degree of the compressed air in the collecting bottle and improving the microorganism in the compressed air to be filtered into the physiological saline of the collecting bottle; through the unidirectional diaphragm that sets up, stop the inside normal saline entering intake pipe of collecting bottle.
The beneficial effects of the invention are as follows:
1. the invention relates to a microorganism sampling device in compressed air, which is provided with a collecting bottle, an air inlet pipe, an air outlet pipe, a top cover and a sampling rod; compressed air enters the bottom of the collecting bottle through the air inlet pipe, so that the compressed air floats upwards from the bottom of the collecting bottle to form physiological saline, and is discharged back to the conveying pipeline from the air outlet pipe; the normal saline cleans the compressed air, so that microorganisms in the compressed air remain in the normal saline and adhere to the surface of the sampling rod; the detection staff takes out the sampling rod to carry out the detection work of microorganism, and the detection staff is convenient for carry out the detection in batches many times to the change condition of microorganism content in the compressed air of in time discovery has improved compressed air's purity degree.
2. The invention relates to a microorganism sampling device in compressed air, which is characterized by comprising a turntable, a motor, a sliding sleeve, a gear and an inner gear ring; the motor drives the carousel to rotate along annular spout, drives a plurality of sampling sticks and rotates in the inside of collecting bottle, and a plurality of gears accompany the carousel rotation, cooperation and its meshed ring gear for gear self rotation drives sampling stick self rotation, thereby makes the sampling stick rotate when accompanying the carousel rotation, has improved efficiency and the degree of consistency that microorganism in the normal saline adsorbed on the sampling stick.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a perspective view of a first embodiment of the present invention;
FIG. 2 is a cross-sectional view of a first embodiment of the invention;
FIG. 3 is an enlarged view of a portion of FIG. 2 at A;
FIG. 4 is a top view of the top cover in accordance with the first embodiment of the present invention;
FIG. 5 is an exploded view of a first embodiment of the present invention;
FIG. 6 is a perspective view of a turntable and rubber roller in accordance with an embodiment of the present invention;
FIG. 7 is a perspective view of a gear, sampling rod, stationary bar and stem according to a first embodiment of the present invention;
FIG. 8 is a block diagram of a sampling wand according to a first embodiment of the present invention;
FIG. 9 is a perspective view of an air inlet pipe and a hollow round seat in a second embodiment of the invention;
FIG. 10 is a cross-sectional view of a hollow circular seat in a second embodiment of the invention;
in the figure: 1. a collection bottle; 2. an air inlet pipe; 3. an air outlet pipe; 4. a top cover; 5. sampling a rod; 6. an annular chute; 7. a turntable; 8. a mounting hole; 9. a cover plate; 10. a motor; 11. a fan-shaped groove; 12. a sliding sleeve; 13. a gear; 14. an inner gear ring; 15. a fixing groove; 16. a fixed rod; 17. a bump; 18. a threaded rod; 19. a round hole; 20. an L-shaped notch; 21. a rotating handle; 22. a cross bar; 23. a mounting groove; 24. rubber rollers; 25. a spring; 26. a sterile rubber sleeve; 27. activated carbon fiber strips; 28. a notch; 29. a soft magnetic stripe; 30. an annular groove; 31. a hollow round seat; 32. a unidirectional diaphragm.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Example 1
As shown in fig. 1 to 2, a device for sampling microorganisms in compressed air according to an embodiment of the present invention includes a collection bottle 1; one side of the collecting bottle 1 is connected with an air inlet pipe 2, and the bottom of the air inlet pipe 2 stretches into the bottom of the collecting bottle 1; the other side of the collecting bottle 1 is connected with an air outlet pipe 3; the air inlet pipe 2 and the air outlet pipe 3 are connected in parallel on a conveying pipeline for preparing compressed air through valves; pure normal saline is filled in the collecting bottle 1; a top cover 4 is arranged on the outer ring thread of the top of the collecting bottle 1; a plurality of sampling rods 5 are uniformly arranged in the middle of the top cover 4, and the sampling rods 5 penetrate through the top cover 4 in a sliding manner; the material of the sampling rod 5 in this embodiment is a material for adsorbing microorganisms, such as amidoxime-based adsorption material; when the compressed air preparation device works, compressed air is conveyed along a conveying pipeline, the compressed air enters the bottom of the collecting bottle 1 through the air inlet pipe 2 by controlling the valve, so that the compressed air floats upwards from the bottom of the collecting bottle 1 to form physiological saline, and then is discharged back to the conveying pipeline from the air outlet pipe 3; the normal saline cleans the compressed air, so that microorganisms in the compressed air remain in the normal saline, and meanwhile, the compressed air floats upwards in the normal saline, so that the normal saline rolls in the collecting bottle 1, and the microorganisms adhere to the surface of the sampling rod 5; the detection personnel take out the sampling rod 5, carry out the detection work of microorganism, be convenient for the detection personnel to carry out the batch detection many times to the change condition of microorganism content in the compressed air has been improved to the timely discovery.
As shown in fig. 1 to 3, an annular chute 6 is formed in the middle of the top cover 4; a turntable 7 is rotatably arranged in the annular chute 6; a plurality of mounting holes 8 are formed in the middle of the turntable 7 in a surrounding mode; the sampling rod 5 is arranged inside the mounting hole 8; the top surface of the top cover 4 is fixedly connected with a cover plate 9; a motor 10 is fixedly connected to the middle part of the top surface of the cover plate 9, and a rotating shaft of the motor 10 rotates to penetrate through the cover plate 9; the rotating shaft of the motor 10 is fixedly connected with the middle part of the turntable 7; the middle part of the cover plate 9 is provided with a plurality of fan-shaped grooves 11 in a surrounding mode, and rubber plugs are slidably arranged in the fan-shaped grooves 11; the width of the circular ring formed by the plurality of the sector grooves 11 is larger than that of the circular ring formed by the plurality of the mounting holes 8, and the circular ring are overlapped; during operation, when compressed air gets into inside the collecting bottle 1, motor 10 drive carousel 7 is rotatory along annular spout 6, drives the inside rotation of a plurality of sampling rod 5 at collecting bottle 1, has improved the efficiency that microorganism in the normal saline adsorbed on the sampling rod 5, through the fan-shaped groove 11 of seting up, is convenient for detect personnel take out sampling rod 5 and install, has improved detection personnel's convenience.
As shown in fig. 2 to 5, the inside of the mounting hole 8 is slidably provided with a sliding sleeve 12; the top end of the sliding sleeve 12 is fixedly connected with a gear 13; the diameter of the inner ring of the gear 13 is the same as that of the inner ring of the sliding sleeve 12, and the gear 13 and the sliding sleeve are communicated in a superposition manner; the sampling rod 5 is arranged on the inner wall of the sliding sleeve 12 in a sliding manner; an inner gear ring 14 is fixedly connected with the outer ring of the top surface of the top cover 4; the outer rings of a plurality of gears 13 are meshed with the inner rings of an inner gear ring 14; during operation, the motor 10 drives the rotary table 7 to rotate along the annular chute 6, drives the gears 13 to rotate along with the rotary table 7, and is matched with the inner gear ring 14 meshed with the gears 13 to enable the gears 13 to rotate to drive the sampling rod 5 to rotate, so that the sampling rod 5 rotates along with the rotation of the rotary table 7, and uniformity of microorganism aggregation on the surface of the sampling rod 5 is improved.
As shown in fig. 3, 5 and 7, a plurality of fixing grooves 15 are formed around the top of the inner ring of the gear 13; a fixed rod 16 is slidably arranged in the sliding sleeve 12; the outer ring at the top of the fixed rod 16 is fixedly connected with a plurality of protruding blocks 17; the convex blocks 17 are in sliding fit with the inner wall of the fixed groove 15; the bottom of the fixed rod 16 is provided with a threaded hole; a threaded rod 18 is fixedly connected to the top end of the sampling rod 5; the threaded rod 18 is in threaded connection with the threaded hole; during operation, when the sampling rod 5 is installed, the threaded rod 18 is installed in the bottom threaded hole of the fixing rod 16, the fixing rod 16 and the sampling rod 5 penetrate through the inner rings of the gear 13 and the sliding sleeve 12, the protruding block 17 slides into the fixing groove 15, the fixing rod 16 and the sampling rod 5 are fixedly installed, accordingly, the installation of detection personnel and the taking out of the sampling rod 5 are facilitated, and the operation convenience of the detection personnel is improved.
As shown in fig. 1, 3, 4, 5 and 7, a round hole 19 is formed at the top end of the fixing rod 16; the two sides of the top of the round hole 19 are provided with L-shaped grooves 20; a rotating handle 21 is arranged in the round hole 19 in a sliding manner; the two sides of the bottom of the rotating handle 21 are fixedly connected with a cross rod 22; the cross bar 22 is in sliding fit with the L-shaped slot 20; the rotating handle 21 penetrates through the fan-shaped groove 11; when the sampling rod 5 is installed and taken out, the rotating handle 21 penetrates through the fan-shaped groove 11, the lower end of the rotating handle 21 is inserted into the round hole 19, the cross rod 22 slides into the L-shaped groove 20, the rotating handle 21 is rotated, the cross rod 22 is driven to rotate into the L-shaped groove 20, and the rotating handle 21 is fixedly connected with the fixed rod 16; thereby facilitating the installation and removal of the sampling wand 5 by the inspector.
As shown in fig. 3, 5 and 6, the outer ring of the turntable 7 is uniformly provided with a plurality of mounting grooves 23 in a surrounding manner; a rubber roller 24 is slidably arranged in the mounting groove 23; the support of the rubber roller 24 is in sliding connection with the inner wall of the mounting groove 23; the outer ring of the rubber roller 24 is in sliding fit with the inner wall of the annular chute 6; a spring 25 is arranged between one surface of the mounting groove 23, which is close to the center of the turntable 7, and the bracket of the rubber roller 24; during operation, the elastic force of the spring 25 pushes the rubber roller 24 to slide towards the outer ring of the rotary table 7 along the mounting groove 23, so that the rubber roller 24 contacts with the inner wall of the annular chute 6, and when the rotary table 7 rotates, the rubber roller 24 is driven to be attached to the annular chute 6 to rotate, so that the friction force during rotation of the rotary table 7 is reduced, and the stability during rotation of the sampling rod 5 is improved.
As shown in fig. 8, the outer ring of the sampling rod 5 is sleeved with a plurality of sterile rubber sleeves 26; a plurality of activated carbon fiber strips 27 are bonded around the outer ring of the sterile rubber sleeve 26; the surface at the sampling rod 5 is established through aseptic rubber sleeve 26 cover, through active carbon fiber strip 27, adsorbs the microorganism in the normal saline at active carbon fiber strip 27's surface, and when the detection personnel detects, only need take off aseptic rubber sleeve 26 and detect to the sample work of the detection personnel of being convenient for, simultaneously, the sampling rod 5 used repeatedly of being convenient for.
As shown in fig. 8, a notch 28 is formed on one side of the sterile rubber sleeve 26; the incision 28 cuts the sterile rubber sleeve 26; a plurality of soft magnetic strips 29 are fixedly connected to two sides of the notch 28; the soft magnetic strips 29 on both sides are mutually attracted; the sterile rubber sleeve 26 is fixed on the outer ring of the sampling rod 5 in a surrounding mode through the mutual attraction of the soft magnetic strips 29 on the two sides, and when a detection person detects, the attracted soft magnetic strips 29 are separated, so that the sterile rubber sleeve 26 is unfolded into a plane, and the detection person can conveniently sample microorganisms.
As shown in fig. 8, the outer ring of the sampling rod 5 is uniformly provided with a plurality of annular grooves 30; the opening direction of the annular groove 30 is inclined downwards from the inner ring to the outer ring; the inner wall of the annular groove 30 is in sliding fit with the inner wall of the sterile rubber sleeve 26; through the annular groove 30 of seting up for aseptic rubber sleeve 26 and active carbon fiber strip 27 are located the inside of annular groove 30, have reduced aseptic rubber sleeve 26 and active carbon fiber strip 27 installation and the probability of taking out the time with sliding sleeve 12 and gear 13 inner race contact, thereby reduced the microorganism of sampling by friction loss and by the probability of external pollution.
Example two
As shown in fig. 9 to 10, comparative example one, in which another embodiment of the present invention is: the lower end of the air inlet pipe 2 is connected with a hollow round seat 31; the top surface of the hollow round seat 31 is provided with a plurality of through grooves in a surrounding manner; the inner top surface of the hollow round seat 31 is fixedly connected with a unidirectional diaphragm 32; the bottom surface of the unidirectional membrane 32 is in sliding fit with the inner bottom surface of the hollow round seat 31; when the air filter works, compressed air is discharged into the hollow round seat 31 along the air inlet pipe 2, the unidirectional diaphragm 32 is pushed to bend upwards, and the compressed air is shunted through the hollow round seat 31 and discharged into the bottom of the collecting bottle 1, so that the dispersion degree of the compressed air in the collecting bottle 1 is improved, and microorganisms in the compressed air are filtered into physiological saline of the collecting bottle 1; the physiological saline inside the collection bottle 1 is blocked from entering the inside of the air inlet pipe 2 by the arranged one-way membrane 32.
Working principle: the sterile rubber sleeve 26 is fixed around the outer ring of the sampling rod 5 through the mutual attraction of the soft magnetic strips 29 at the two sides, so that the sterile rubber sleeve 26 and the activated carbon fiber strips 27 are positioned in the annular groove 30; the lower end of the rotary handle 21 is inserted into the round hole 19 through the threaded rod 18 in the threaded hole at the bottom of the fixed rod 16, so that the cross rod 22 slides into the L-shaped groove 20, the rotary handle 21 is rotated, the cross rod 22 is driven to rotate into the L-shaped groove 20, and the rotary handle 21 is fixedly connected with the fixed rod 16; the inspector holds the rotating handle 21, and passes the fixed rod 16 and the sampling rod 5 through the gear 13 and the inner ring of the sliding sleeve 12, so that the convex block 17 slides into the fixed groove 15; the rotating handle 21 is reversely rotated, and the rotating handle 21 is taken out;
injecting sterile physiological saline into the inside of the collection bottle 1; the compressed air is conveyed along the conveying pipeline, and enters the bottom of the collecting bottle 1 through the air inlet pipe 2 by controlling the valve, so that the compressed air floats upwards from the bottom of the collecting bottle 1 to form physiological saline, and is discharged back to the conveying pipeline from the air outlet pipe 3; the normal saline cleans the compressed air, so that microorganisms in the compressed air remain in the normal saline, and meanwhile, the compressed air floats upwards in the normal saline, so that the normal saline rolls in the collecting bottle 1; the motor 10 drives the rotary table 7 to rotate along the annular chute 6 to drive the sampling rods 5 to rotate in the collecting bottle 1, the gears 13 rotate along with the rotary table 7 and match with the inner gear ring 14 meshed with the gears 13 to enable the gears 13 to rotate to drive the sampling rods 5 to rotate, so that organisms in physiological saline are accumulated on the surface of the sampling rods 5, particularly on the surface of the activated carbon fiber strips 27;
when a detector performs sampling detection, the rotating handle 21 is used for taking out the fixed rod 16 and the sampling rod 5, and the sucked soft magnetic strips 29 are separated, so that the sterile rubber sleeve 26 is unfolded into a plane, and the sampling work of the detector on microorganisms is facilitated; the method is convenient for detection personnel to detect in batches for many times, so that the change condition of the microorganism content in the compressed air can be found in time, and the purity of the compressed air is improved.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. A microorganism sampling device in compressed air, which is characterized in that: comprising a collecting bottle (1); one side of the collecting bottle (1) is connected with an air inlet pipe (2), and the bottom of the air inlet pipe (2) extends into the bottom of the collecting bottle (1); the other side of the collecting bottle (1) is connected with an air outlet pipe (3); the air inlet pipe (2) and the air outlet pipe (3) are connected in parallel on a conveying pipeline prepared from compressed air through a valve; pure normal saline is filled in the collecting bottle (1); a top cover (4) is arranged on the outer ring of the top of the collecting bottle (1) in a threaded manner; a plurality of sampling rods (5) are uniformly arranged in the middle of the top cover (4), and the sampling rods (5) penetrate through the top cover (4) in a sliding manner;
an annular chute (6) is formed in the middle of the top cover (4); a turntable (7) is rotatably arranged in the annular chute (6); a plurality of mounting holes (8) are formed around the middle part of the turntable (7); the sampling rod (5) is arranged in the mounting hole (8); the top surface of the top cover (4) is fixedly connected with a cover plate (9); a motor (10) is fixedly connected in the middle of the top surface of the cover plate (9), and a rotating shaft of the motor (10) rotates to penetrate through the cover plate (9); the rotating shaft of the motor (10) is fixedly connected with the middle part of the turntable (7); the middle part of the cover plate (9) is surrounded by a plurality of fan-shaped grooves (11), and rubber plugs are slidably arranged in the fan-shaped grooves (11); the width of the circular ring formed by the plurality of the sector grooves (11) is larger than that of the circular ring formed by the plurality of the mounting holes (8), and the circular ring are overlapped;
a sliding sleeve (12) is arranged in the mounting hole (8) in a sliding manner; the top end of the sliding sleeve (12) is fixedly connected with a gear (13); the diameter of the inner ring of the gear (13) is the same as that of the inner ring of the sliding sleeve (12), and the gear and the sliding sleeve are communicated in a superposition manner; the sampling rod (5) is arranged on the inner wall of the sliding sleeve (12) in a sliding manner; an inner gear ring (14) is fixedly connected with the outer ring of the top surface of the top cover (4); an outer ring of the plurality of gears (13) is meshed with an inner ring of the inner gear ring (14);
a plurality of fixing grooves (15) are formed in the top of the inner ring of the gear (13) in a surrounding mode; a fixed rod (16) is arranged in the sliding sleeve (12) in a sliding manner; the top outer ring of the fixed rod (16) is fixedly connected with a plurality of convex blocks (17); the convex blocks (17) are in sliding fit with the inner wall of the fixed groove (15); the bottom of the fixed rod (16) is provided with a threaded hole; a threaded rod (18) is fixedly connected to the top end of the sampling rod (5); the threaded rod (18) is in threaded connection with the threaded hole;
a round hole (19) is formed in the top end of the fixed rod (16); the two sides of the top of the round hole (19) are provided with L-shaped notches (20); a rotating handle (21) is arranged in the round hole (19) in a sliding manner; the two sides of the bottom of the rotating handle (21) are fixedly connected with cross bars (22); the cross rod (22) is in sliding fit with the L-shaped groove opening (20); the rotating handle (21) penetrates through the fan-shaped groove (11);
the outer ring of the sampling rod (5) is sleeved with a plurality of sterile rubber sleeves (26); the outer ring of the sterile rubber sleeve (26) is bonded with a plurality of activated carbon fiber strips (27) in a surrounding manner;
a notch (28) is formed in one side of the sterile rubber sleeve (26); the incision (28) cuts the sterile rubber sleeve (26); a plurality of soft magnetic strips (29) are fixedly connected to two sides of the notch (28); the soft magnetic strips (29) on the two sides are mutually attracted;
a plurality of annular grooves (30) are uniformly formed in the outer ring of the sampling rod (5); the opening direction of the annular groove (30) is inclined downwards from the inner ring to the outer ring; the inner wall of the annular groove (30) is in sliding fit with the inner wall of the sterile rubber sleeve (26).
2. A compressed air microorganism sampling apparatus according to claim 1, wherein: a plurality of mounting grooves (23) are uniformly formed around the outer ring of the turntable (7); a rubber roller (24) is slidably arranged in the mounting groove (23); the support of the rubber roller (24) is in sliding connection with the inner wall of the mounting groove (23); the outer ring of the rubber roller (24) is in sliding fit with the inner wall of the annular chute (6); a spring (25) is arranged between one surface of the mounting groove (23) close to the center of the turntable (7) and the support of the rubber roller (24).
3. A compressed air microorganism sampling apparatus according to claim 1, wherein: the bottom end of the air inlet pipe (2) is connected with a hollow round seat (31); the top surface of the hollow round seat (31) is provided with a plurality of through grooves in a surrounding way; the inner top surface of the hollow round seat (31) is fixedly connected with a unidirectional diaphragm (32); the bottom surface of the unidirectional diaphragm (32) is in sliding fit with the inner bottom surface of the hollow round seat (31).
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| CN202310387418.1A CN116240096B (en) | 2023-04-11 | 2023-04-11 | Microorganism sampling device in compressed air |
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| CN202310387418.1A CN116240096B (en) | 2023-04-11 | 2023-04-11 | Microorganism sampling device in compressed air |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106324052A (en) * | 2016-08-19 | 2017-01-11 | 李宗珍 | Testing system for detecting microorganism in compression gas |
| CN206074314U (en) * | 2016-08-29 | 2017-04-05 | 昆明中药厂有限公司 | A kind of multifunctional compressed air sampling detecting device |
| CN110514483A (en) * | 2019-08-13 | 2019-11-29 | 湖北华强科技有限责任公司 | A kind of fast appraisement method of Compressed Air degree |
| CN211317889U (en) * | 2019-12-12 | 2020-08-21 | 博迈医疗科技(常州)有限公司 | Compressed air collecting device |
| CN217442911U (en) * | 2022-05-19 | 2022-09-16 | 武汉凤梧科技有限公司 | Gaseous for integrated analysis appearance gas sampling device of SF6 |
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2023
- 2023-04-11 CN CN202310387418.1A patent/CN116240096B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106324052A (en) * | 2016-08-19 | 2017-01-11 | 李宗珍 | Testing system for detecting microorganism in compression gas |
| CN206074314U (en) * | 2016-08-29 | 2017-04-05 | 昆明中药厂有限公司 | A kind of multifunctional compressed air sampling detecting device |
| CN110514483A (en) * | 2019-08-13 | 2019-11-29 | 湖北华强科技有限责任公司 | A kind of fast appraisement method of Compressed Air degree |
| CN211317889U (en) * | 2019-12-12 | 2020-08-21 | 博迈医疗科技(常州)有限公司 | Compressed air collecting device |
| CN217442911U (en) * | 2022-05-19 | 2022-09-16 | 武汉凤梧科技有限公司 | Gaseous for integrated analysis appearance gas sampling device of SF6 |
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