CN1162388C - Extract of free anthraquinone extracted from rhubarb and preparation process thereof - Google Patents
Extract of free anthraquinone extracted from rhubarb and preparation process thereof Download PDFInfo
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- CN1162388C CN1162388C CNB001254057A CN00125405A CN1162388C CN 1162388 C CN1162388 C CN 1162388C CN B001254057 A CNB001254057 A CN B001254057A CN 00125405 A CN00125405 A CN 00125405A CN 1162388 C CN1162388 C CN 1162388C
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- anthraquinone
- rhubarb
- rheum officinale
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Abstract
The present invention relates to Chinese medicine effective component extractive and a preparation method thereof, particularly to extractum of dissociation anthraquinone extracted from rhubarb, and a preparation method of the extractum. The extractum of the dissociation anthraquinones is extracted from rhubarb, rhababerone extractive is processed by hydrolysis to make combined anthraquinones fully converted into the dissociation anthraquinones; the effective component content of the extractum is increased, the selectivity of drug effect is strong, and the side reaction is little. The preparation process of the extractum has simple operation, and toxic and harmful solvents are not used; the present invention is suitable for large-scale industrial production, and promotes the development and the utilization of the effective components of the rhubarb to get on a new stage.
Description
The present invention relates to Chinese medicine effective component extracts and preparation method, be specifically related to from rheum officinale, extract the medicinal extract and the preparation method of dissociated anthraquinone.
Rheum officinale is world-famous traditional Chinese medicine, and the beginning is stated from Shennong's Herbal, is one of important drugs of the multiple disease of treatment.The modern degree of depth and range to its pharmacological effect, chemical ingredients and clinical study are that other Chinese medicine is difficult to compare.Rheum officinale has and rushes down down, anti-bacteria and anti-virus, antipsychotic, antitumor, analgesic, hemostasis, anti-inflammatory analgesic, improves multiple pharmacological effect such as renal insufficiency, has been widely used in multiple treatment of diseases such as chronic renal failure, upper gastrointestinal hemorrhage, acute pancreatitis, acute ileus, hepatitis gravis.Rheum officinale mainly contains anthraquinone and dimerization anthraquinone analog compound.The anthraquinone class comprises Schuttgelb, chrysophanol, cassia angustifolia glucoside, rhabarberone, rhubarb yellow, physcion.15 kinds of-catechin, gallic acids etc.Other still has diphenylethylene, phenyl phthalein ketone, tannin, naphthalene class, chromone etc. to amount to 80 kinds of compounds, and various trace elements such as calcium, iron, magnesium.Anthraquinone analog compound is the main effective constituent of rheum officinale, is that anthraquinone glycoside and dissociated anthraquinone are that the anthraquinone glycoside n-ary form n exists with combined anthraquinone in plant.
The main pharmacological of Radix Et Rhizoma Rhei anthraquinone analog derivative is: 1. discharge function: after the rheum officinale oral administration, the combined anthraquinone glucoside is because of there being the protection of glycosyl, most ofly directly arrives large intestine without absorbing, and it is first and sugared to be resolved into glucoside by bacterial enzyme in intestines.Glucoside unit stimulates big intestinal mucosa, and suppresses sodium ion and absorb from enteric cavity, makes that moisture increases in the large intestine, and hyperperistalsis and causing is rushed down.2. anti-microbial effect: the dissociated anthraquinone compounds has broad-spectrum antimicrobial, antivirus action, can alleviate intracellular toxin in the blood, impels the adrenocortical hormone secretion, helps the antiviral stress reaction of anti-inflammatory behind the organism infection.Dysentery bacterium, typhoid fever bacterium, cholera bacteria etc. there is stronger restraining effect.This anti-microbial effect is owing to the biosynthesizing that hinders bacterium proteinoid and nucleic acid produces, and this will make bacterium be difficult for producing resistance.Bacteriostatic effective constituent mainly is Schuttgelb, rhubarb yellow and rhabarberone.3. anastalsis: Radix Et Rhizoma Rhei anthraquinone has many-sided anastalsis.Chrysophanol can reduce the permeability of capillary vessel, reduces ulcer surface and oozes out, and improves vascular fragility, shortens the clotting time, hemopoietic.Same Schuttgelb also has tangible short hemagglutinative function.Along with the further investigation to rheum officinale, the pharmacological action of Radix Et Rhizoma Rhei anthraquinone will be constantly revealed.Can learn also that thus combined anthraquinone is different with dissociated anthraquinone absorption in vivo pathways metabolism with effect, combined anthraquinone is broken down into dissociated anthraquinone and works in large intestine, mainly based on defaecation; Dissociated anthraquinone absorbs by stomach mucous membrane under one's belt and enters blood, thereby plays antibiotic and antiviral effect.
At present, rheum officinale mostly is ethanol extraction or the direct preparation of crude drug, comprising rhubarb tablets, smart pornographic movie, rheum officinale Tongbian Chongji Zhu Yan Yang Lu Ma Jian, aromatic tincture of rhubarb etc.The effective constituent of these preparations is based on combined anthraquinone, and contains a spot of dissociated anthraquinone.When rhubarb preparation is used as antibiotic, hemostasis or during other purposes, it rushes down down that effect just becomes side effect.And total anthraquinones content (comprising combined anthraquinone and dissociated anthraquinone) only is 20% in the present Radix Et Rhizoma Rhei extract.
The object of the present invention is to provide a kind of medicinal extract that contains the dissociated anthraquinone single component that from rheum officinale, extracts, Radix Et Rhizoma Rhei extract curative effect selectivity is increased, reduce side reaction.
The present invention extracts dissociated anthraquinone from rheum officinale medicinal extract contains activeconstituents dissociated anthraquinone 〉=50%.
Another object of the present invention is to disclose the above-mentioned extract making method of from rheum officinale, extracting dissociated anthraquinone.
The present invention extracts dissociated anthraquinone from rheum officinale medicinal extract makes through the following steps:
The rheum officinale crude drug is pulverized into meal, 40～95% alcohol immersion 4～20 hours that add 6～12 times of amounts, heating and refluxing extraction three times, return time 0.5～2 hour, the elimination dregs of a decoction, united extraction liquid adds 0.5～50ml diluted acid by the 100g crude drug, adds acid, concentrating under reduced pressure under 35～80 ℃ of temperature, reclaim ethanol, when treating solution concentration to the thick paste shape, cold filtration, leaching thing washes with water, again with a small amount of 80～99% washing with alcohol, take out solids and be drying to obtain Radix Et Rhizoma Rhei anthraquinone medicinal extract, wherein dissociated anthraquinone content 〉=50% after draining.
Diluted acid of the present invention is meant with 1: the mineral acid that 0.5-10 doubly dilutes, and as sulfuric acid, hydrochloric acid etc.
The present invention extracts the medicinal extract of dissociated anthraquinone from rheum officinale, by hydrolysis, make combined anthraquinone all be converted into dissociated anthraquinone the rheum officinale alcohol extract, and Radix Et Rhizoma Rhei extract anthraquinone glycoside unit content 〉=50% of acquisition can directly be made enteric coated tablet and play defaecation, discharge function; Make ordinary tablet and promptly eliminated causing of rheum officinale and rush down side effect, make antibiotic, antivirus action is more remarkable; Because of the active constituent content height also can be made into the usefulness of injection liquid for clinical acute disease.
The medicinal extract active constituent content height that from rheum officinale, extracts dissociated anthraquinone disclosed by the invention, the drug effect selectivity is strong, and side reaction is little; Preparation technology is easy and simple to handle, does not use the poisonous and harmful solvent, is fit to large-scale commercial production, for the effective rhubarb component development and use step on a new stage.
Embodiment one
Get rheum officinale crude drug 150g, add 95% ethanol 1500ml and soaked 12 hours, heating and refluxing extraction three times was extracted 1 hour at every turn, the elimination dregs of a decoction, and united extraction liquid adds Hcl (1: 1) 5ml, and concentrating under reduced pressure under 50 ℃ condition reclaims ethanol.When solution concentration during to the thick paste shape, cold filtration leaches thing and washes with water, again with a small amount of 95% washing with alcohol, takes out solids and is drying to obtain after draining, and wherein dissociated anthraquinone content is 93%, yield 0.15%.
Embodiment two
Get rheum officinale crude drug 150g, add 95% ethanol 1500ml and soaked 12 hours, heating and refluxing extraction three times was extracted 1 hour at every turn, the elimination dregs of a decoction, and united extraction liquid adds Hcl (1: 1) 10ml, and concentrating under reduced pressure under 70 ℃ condition reclaims ethanol.When solution concentration during to the thick paste shape, cold filtration leaches thing and washes with water, again with a small amount of 95% washing with alcohol, takes out solids and is drying to obtain after draining, and wherein dissociated anthraquinone content is 83.4%, yield 0.51%.
Embodiment three
Get rheum officinale crude drug 150g, add 95% ethanol 1500ml and soaked 12 hours, heating and refluxing extraction three times was extracted 1 hour at every turn, the elimination dregs of a decoction, and united extraction liquid adds 20%H
2SO
410ml, concentrating under reduced pressure under 30 ℃ condition reclaims ethanol.When solution concentration during to the thick paste shape, cold filtration leaches thing and washes with water, again with a small amount of 95% washing with alcohol, takes out solids and is drying to obtain after draining, and wherein dissociated anthraquinone content is 72.5%, yield 0.78%.
Claims (1)
1, a kind of method of extracting dissociated anthraquinone medicinal extract from rheum officinale is characterized in that this method comprises the following steps:
The rheum officinale crude drug is pulverized into meal, 40～95% alcohol immersion 4～20 hours that add 6～12 times of amounts, heating and refluxing extraction three times, return time 0.5～2 hour, the elimination dregs of a decoction, united extraction liquid, press the 100g crude drug and add the 0.5-50ml diluted acid, concentrating under reduced pressure under 35～80 ℃ of temperature reclaims ethanol, when treating solution concentration to the thick paste shape, cold filtration leaches thing and washes with water, after draining again with a small amount of 80～99% washing with alcohol, take out solids and be drying to obtain Radix Et Rhizoma Rhei anthraquinone medicinal extract, wherein said diluted acid is meant with 1: the mineral acid that 0.5-10 doubly dilutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001254057A CN1162388C (en) | 2000-09-26 | 2000-09-26 | Extract of free anthraquinone extracted from rhubarb and preparation process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001254057A CN1162388C (en) | 2000-09-26 | 2000-09-26 | Extract of free anthraquinone extracted from rhubarb and preparation process thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1345597A CN1345597A (en) | 2002-04-24 |
| CN1162388C true CN1162388C (en) | 2004-08-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB001254057A Expired - Fee Related CN1162388C (en) | 2000-09-26 | 2000-09-26 | Extract of free anthraquinone extracted from rhubarb and preparation process thereof |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100370993C (en) * | 2006-01-11 | 2008-02-27 | 四川省中药研究所 | Medicinal composition with heat-clearing, fire-draining and detoxification function |
| PL2346517T4 (en) * | 2008-10-31 | 2016-10-31 | Plant extract hydrolysates and antibacterial product containing the same | |
| CN103127252B (en) * | 2013-03-13 | 2015-03-11 | 四川省中医药科学院 | Composition for male sexual function health-care |
| CN103229895A (en) * | 2013-05-22 | 2013-08-07 | 湖南省五季风生物科技有限责任公司 | Rheum officinale extract and application thereof |
| CN105123789B (en) * | 2015-09-25 | 2017-10-20 | 兰州大学 | A kind of free anthraquinones extracted from rheum nano-emulsion preparation for being used to prevent and treat plant red spider disease |
| CN111346075A (en) * | 2020-03-24 | 2020-06-30 | 深圳市金汇球高科技有限公司 | Application of rhubarb anthraquinone extract in broad-spectrum antiviral |
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2000
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| CN1345597A (en) | 2002-04-24 |
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Granted publication date: 20040818 Termination date: 20130926 |