CN116223720A - Medical magnesium alloy material in-vitro degradation rate experimental device and method - Google Patents
Medical magnesium alloy material in-vitro degradation rate experimental device and method Download PDFInfo
- Publication number
- CN116223720A CN116223720A CN202310246741.7A CN202310246741A CN116223720A CN 116223720 A CN116223720 A CN 116223720A CN 202310246741 A CN202310246741 A CN 202310246741A CN 116223720 A CN116223720 A CN 116223720A
- Authority
- CN
- China
- Prior art keywords
- magnesium alloy
- alloy material
- medical magnesium
- burette
- degradation rate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229910000861 Mg alloy Inorganic materials 0.000 title claims abstract description 101
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 91
- 230000015556 catabolic process Effects 0.000 title claims abstract description 89
- 239000000956 alloy Substances 0.000 title claims abstract description 84
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000000338 in vitro Methods 0.000 title claims abstract description 30
- 239000012890 simulated body fluid Substances 0.000 claims abstract description 53
- 238000007789 sealing Methods 0.000 claims abstract description 28
- 238000002474 experimental method Methods 0.000 claims abstract description 16
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000001257 hydrogen Substances 0.000 claims abstract description 15
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 15
- 230000008859 change Effects 0.000 claims abstract description 9
- 239000000523 sample Substances 0.000 claims description 24
- 239000000463 material Substances 0.000 claims description 17
- 238000002791 soaking Methods 0.000 claims description 16
- 239000011777 magnesium Substances 0.000 claims description 14
- 230000004580 weight loss Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 10
- 239000007857 degradation product Substances 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 7
- 239000012496 blank sample Substances 0.000 claims description 4
- 239000007943 implant Substances 0.000 description 7
- 239000010839 body fluid Substances 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 238000012404 In vitro experiment Methods 0.000 description 1
- 229910019440 Mg(OH) Inorganic materials 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/20—Metals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/36—Hydrogen production from non-carbon containing sources, e.g. by water electrolysis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Instructional Devices (AREA)
Abstract
The invention relates to the field of medical treatment, in particular to an in-vitro degradation rate experimental device and method for medical magnesium alloy materials. The SBF simulated body fluid container, the burette, the sealing cover, the cover body, the sealable hose and the like are matched, so that the degradation experiment of the medical magnesium alloy material experimental body can be conveniently carried out, and the medical magnesium alloy material experimental body has the characteristics of simple structure, convenience in use and operation and high accuracy. When the medical magnesium alloy tube is used, firstly, SBF simulated body fluid is poured into an SBF simulated body fluid container, a medical magnesium alloy material experimental body is placed in the tube body, the whole tube body is covered by a cover body, and the tail end of the inverted funnel-shaped cover body is connected with a burette; vacuumizing the inside of the burette, sucking SBF simulated body fluid to the highest volume scale of the burette, and sealing the sealable hose; recording the scale change of SBF simulated body fluid level drop according to a preset time interval to obtain the hydrogen generation amount of the medical magnesium alloy material experimental body, namely calculating the instantaneous degradation rate V and the average degradation rate C of the medical magnesium alloy material experimental body.
Description
Technical Field
The invention relates to the field of medical treatment, in particular to an in-vitro degradation rate experimental device and method for medical magnesium alloy materials.
Background
The biomedical magnesium alloy material is used as a novel medical degradable implant material, has excellent mechanical property and degradability, but is used for human body without toxic or side effect, which requires the biological material to have high purity, the impurity content is PPM level, and no disease and no toxic propagation condition is ensured. Meanwhile, the compound and monomer impurities, degradation or abrasion products do not have adverse effects on the body, and the compound has certain in-vivo adaptability: including adaptability to other materials in medical supplies, biocompatibility, blood compatibility and tissue compatibility of the materials with human bodies, and long-term no influence on body fluid is required after the materials are implanted into human bodies.
Medical magnesium alloy implant materials with low purity often undergo excessively rapid degradation before tissue recovery, and are difficult to meet the physical, chemical and mechanical performance requirements of the design and functions required by medical use, so that the clinical application of the magnesium alloy is limited, and therefore, the medical magnesium alloy implant materials which are easy to produce hydrogen evolution degradation are usually required to be tested before the medical degradable biological materials are used, and the degradation rate of the medical magnesium alloy implant materials is analyzed.
Compared with in vivo experiments and clinical researches of magnesium alloy implant materials, the in vitro experiments can more quickly and intuitively obtain degradation conclusion. At present, a common research method is an in-vitro dynamic soaking experiment, namely, a medical magnesium alloy implant material is soaked in SBF simulated body fluid, so that the degradation rate of the medical magnesium alloy implant material is judged, the dynamic soaking experiment generally adopts a method of manually drying a sample and weighing the sample to calculate the degradation rate (the weight of metal material loss per unit area in unit time of the degradation rate or the average concentration thickness of metal material loss in unit time), and no proper device is used for carrying out the experiment. The method has the defects of inconvenient operation, time and labor waste and low accuracy. Therefore, a special experimental device is needed to be designed to be matched with a corresponding method for experiment.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides an in-vitro degradation rate experimental device and method for a medical magnesium alloy material.
One of the technical schemes adopted by the invention is as follows: the medical magnesium alloy material in-vitro degradation rate experimental device comprises an SBF simulated body fluid container and a burette, wherein the upper part of the SBF simulated body fluid container is provided with a sealing cover, and the lower part of the burette penetrates through the sealing cover and then stretches into the SBF simulated body fluid container; a cover body for covering a medical magnesium alloy material experimental body is arranged at the bottom end of the burette, and a sealable hose is arranged at the top end of the burette; volume graduation lines are arranged on the burette.
In order to better realize the invention, the bottom of the inside of the SBF simulated body fluid container is provided with a cylinder for placing a medical magnesium alloy material experimental body, the top of the cylinder is opened, and the position of the opening corresponds to the bottom position of the burette.
In order to better realize the invention, a plurality of through liquid inlet holes are arranged on the cylinder body.
In order to better realize the invention, the sealing cover is provided with a PH detection hole which is convenient for PH detection and is matched with a corresponding plug.
In order to better realize the invention, sealing rubber rings are arranged at the matching position of the sealing cover and the SBF simulated body fluid container and the matching position of the sealing cover and the burette.
In order to better realize the invention, the cover body is in an inverted funnel shape.
One of the technical schemes adopted by the invention is as follows: the in-vitro degradation rate experimental method for the medical magnesium alloy material adopts the in-vitro degradation rate experimental device for the medical magnesium alloy material, and comprises the following steps:
a. pouring SBF simulated body fluid into an SBF simulated body fluid container, placing a medical magnesium alloy material experimental body into a cylinder, then buckling an inverted funnel-shaped cover body to cover the whole cylinder, and connecting a burette at the tail end of the inverted funnel-shaped cover body;
b. the upper end of the burette is connected with a sealable hose, the sealable hose is connected with a vacuumizing device to vacuumize the interior of the burette and suck SBF simulated body fluid to the highest volume scale of the burette, and then the sealable hose is sealed;
c. standing the whole device, recording the scale change of SBF simulated body fluid level drop according to a preset time interval, obtaining the hydrogen generation amount of the medical magnesium alloy material experimental body, and then calculating the instantaneous degradation rate V and the average degradation rate C of the medical magnesium alloy material experimental body.
In order to better implement the present invention, in the step c, the instantaneous degradation rate v= (W) 0 -W 1 -W 2 ) AT, wherein W 0 Mg is the mass of the medical magnesium alloy material experimental body before degradation; w (W) 1 Mg is the mass of the sample after degradation and degradation products are removed; w (W) 2 Correcting the weight loss of the blank sample of the medical magnesium alloy material experimental body after degradation products are removed from the medical magnesium alloy material experimental body and the medical magnesium alloy material experimental body in the same batch, wherein mg is the weight loss; a is the surface area of the sample, cm 2 The method comprises the steps of carrying out a first treatment on the surface of the T is the sample soaking time, and h.
In order to better realize the invention, in the step C, the average degradation rate C=K delta m/rho At of the medical magnesium alloy material experimental body is shown in the formula; k is well known, 8.76X10 4 The method comprises the steps of carrying out a first treatment on the surface of the Δm is the mass lost by the sample, g; ρ is the density of the material, g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the A is the initial surface area of the sample in cm 2 The method comprises the steps of carrying out a first treatment on the surface of the t is soaking time, h.
The beneficial effects of the invention are as follows: according to the medical magnesium alloy material in-vitro degradation rate experimental device and method, the SBF is used for simulating the matching of a body fluid container, a burette, a sealing cover, a cover body, a sealable hose and the like, so that the degradation experiment of a medical magnesium alloy material experimental body can be conveniently carried out, and the medical magnesium alloy material in-vitro degradation rate experimental device and method have the characteristics of simple structure, convenience in use and operation and high accuracy. When the medical magnesium alloy tube is used, firstly, the SBF simulated body fluid is poured into the SBF simulated body fluid container, a medical magnesium alloy material experimental body is placed in the tube body, then the whole tube body is covered by the inverted funnel-shaped cover body in a buckled manner, and the tail end of the inverted funnel-shaped cover body is connected with a burette; the upper end of the burette is connected with a sealable hose, the sealable hose is connected with a vacuumizing device to vacuumize the interior of the burette and suck SBF simulated body fluid to the highest volume scale of the burette, and then the sealable hose is sealed; and (3) standing the whole device, recording the scale change of the SBF simulated body fluid level drop according to a preset time interval, and obtaining the hydrogen generation amount of the medical magnesium alloy material experimental body, namely calculating the instantaneous degradation rate V and the average degradation rate C of the medical magnesium alloy material experimental body.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. Like elements or portions are generally identified by like reference numerals throughout the several figures. In the drawings, elements or portions thereof are not necessarily drawn to scale.
FIG. 1 is a schematic structural diagram of an experimental device for in vitro degradation rate of medical magnesium alloy material;
FIG. 2 is a schematic diagram of a structure of a burette and a cover of the medical magnesium alloy material in-vitro degradation rate experiment device;
FIG. 3 is a schematic structural view of the SBF simulated body fluid container of the medical magnesium alloy material in vitro degradation rate experimental device of the invention in section when matched with a cylinder;
in the drawings, 1-SBF simulates a body fluid container, 2-sealing cover, 3-PH detection hole, 4-cylinder, 5-cover, 6-burette, 7-sealable hose and 8-liquid inlet.
Detailed Description
Embodiments of the technical scheme of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical aspects of the present invention, and thus are merely examples, and are not intended to limit the scope of the present invention.
It is noted that unless otherwise indicated, technical or scientific terms used herein should be given the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.
Examples:
as shown in fig. 1 to 3, the medical magnesium alloy material in-vitro degradation rate experimental device and method comprise an SBF simulated body fluid container 1 and a burette 6, wherein the upper part of the SBF simulated body fluid container 1 is provided with a sealing cover 2, and the lower part of the burette 6 penetrates through the sealing cover 2 and then stretches into the SBF simulated body fluid container 1; a cover body 5 for covering a medical magnesium alloy material experimental body is arranged at the bottom end of the burette 6, and a sealable hose 7 is arranged at the top end of the burette 6; volume graduation marks are arranged on the burette 6. According to the medical magnesium alloy material in-vitro degradation rate experimental device and method, the SBF is used for simulating the matching of the body fluid container 1, the burette 6, the sealing cover 2, the cover body 5, the sealable hose 7 and the like, so that the degradation experiment of a medical magnesium alloy material experimental body can be conveniently carried out, and the medical magnesium alloy material in-vitro degradation rate experimental device and method have the characteristics of simple structure, convenience in use and operation and high accuracy. When the medical magnesium alloy tube is used, firstly, SBF simulated body fluid is poured into an SBF simulated body fluid container 1, a medical magnesium alloy material experimental body is placed in a tube body 4, then an inverted funnel-shaped cover body 5 is buckled and covers the whole tube body 4, and the tail end of the inverted funnel-shaped cover body 5 is connected with a burette 6; a sealable hose 7 is connected to the upper end of the burette 6, the sealable hose 7 is connected with a vacuumizing device for vacuumizing the interior of the burette 6 and sucking SBF simulated body fluid to the highest volume scale of the burette 6, and then the sealable hose 7 is sealed; and (3) standing the whole device, recording the scale change of the SBF simulated body fluid level drop according to a preset time interval, and obtaining the hydrogen generation amount of the medical magnesium alloy material experimental body, namely calculating the instantaneous degradation rate V and the average degradation rate C of the medical magnesium alloy material experimental body.
Preferably, a cylinder 4 for placing a medical magnesium alloy material experimental body is arranged at the bottom of the inside of the SBF simulated body fluid container 1, and the top of the cylinder 4 is opened and the position of the opening corresponds to the bottom position of the burette 6. The cylinder body 4 is provided with a plurality of through liquid inlet holes 8, so that SBF simulated body fluid can conveniently enter and fully contact with a medical magnesium alloy material experimental body.
Preferably, the sealing cover 2 is provided with a PH detection hole 3 for facilitating PH detection, and is matched with a corresponding plug, so that the PH value can be conveniently detected. The weight loss of the medical magnesium alloy material experimental body is measured and used for calculating the degradation rate and monitoring the PH change condition to double verify degradation conditions.
Preferably, sealing rubber rings are arranged at the matching position of the sealing cover 2 and the SBF simulated body fluid container 1 and the matching position of the sealing cover 2 and the burette 6, so that the sealing performance is improved, and the experimental accuracy is ensured. Preferably, the cover 5 is in the shape of an inverted funnel.
The in-vitro degradation rate experimental method for the medical magnesium alloy material adopts the in-vitro degradation rate experimental device for the medical magnesium alloy material, and comprises the following steps:
a. pouring SBF simulated body fluid into the SBF simulated body fluid container 1, placing a medical magnesium alloy material experimental body into the cylinder 4, then buckling an inverted funnel-shaped cover body 5 to cover the whole cylinder 4, and connecting the tail end of the inverted funnel-shaped cover body 5 with the lower end of a burette 6;
b. a sealable hose 7 is connected to the upper end of the burette 6, the sealable hose 7 is connected with a vacuumizing device for vacuumizing the interior of the burette 6 and sucking SBF simulated body fluid to the highest volume scale of the burette 6, and then the sealable hose 7 is sealed;
c. standing the whole device, recording the scale change of SBF simulated body fluid level drop according to a preset time interval, obtaining the hydrogen generation amount of the medical magnesium alloy material experimental body, and then calculating the instantaneous degradation rate V and the average degradation rate C of the medical magnesium alloy material experimental body.
In the step c, the instantaneous degradation rate V= (W) 0 -W 1 -W 2 ) AT, wherein W 0 Mg is the mass of the medical magnesium alloy material experimental body before degradation; w (W) 1 Mg is the mass of the sample after degradation and degradation products are removed; w (W) 2 Correcting the weight loss of the blank sample of the medical magnesium alloy material experimental body after degradation products are removed from the medical magnesium alloy material experimental body and the medical magnesium alloy material experimental body in the same batch, wherein mg is the weight loss; a is the surface area of the sample, cm 2 The method comprises the steps of carrying out a first treatment on the surface of the T is the sample soaking time, and h.
In the step C, the average degradation rate C=K delta m/rho At of the medical magnesium alloy material experimental body is shown in the formula; k is well known, 8.76X10 4 The method comprises the steps of carrying out a first treatment on the surface of the Δm is the mass lost by the sample, g; ρ is the density of the material, g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the A is the initial surface area of the sample in cm 2 The method comprises the steps of carrying out a first treatment on the surface of the t is soaking time, h.
As a preferred embodiment, FIG. 1 is a schematic structural diagram of an in vitro degradation rate experiment device for medical magnesium alloy materials, which comprises an SBF simulated body fluid container 1, a burette 6, a sealing cover 2, a cover body 5, a sealable hose 7 and a cylinder 4, wherein the bottom end of the burette 6 is lower than the liquid level of the SBF simulated body fluid in the SBF simulated body fluid container 1 so as to realize liquid sealing.
The medical magnesium alloy material experimental body is placed in the cylinder 4, the cover body 5 is reversely buckled in the SBF simulated body fluid container 1 containing the SBF simulated body fluid, the top of the cover body 5 is connected with the burette 6, the bottom end of the burette 6 is lower than the liquid level of the SBF simulated body fluid solution in the beaker, and the burette 6 can select different volumes of 50ml or 100ml according to different degradation rates of the medical magnesium alloy material experimental body. The inside of burette 6 is evacuated with a pump or other evacuation device and SBF-simulated body fluid is sucked up to the highest scale of burette 6, after which burette 6 is sealed.
Hydrogen generated by degrading the medical magnesium alloy material experimental body enters the space above the liquid level of the burette 6 from the funnel-shaped cover body 5 in a light throwing mode, part of SBF simulated body fluid solution in the burette 6 is replaced by the hydrogen to cause the SBF simulated body fluid solution in the burette 6 to descend, and the collected hydrogen can be directly reacted through the descending degree of the liquid level in the burette 6 by scale change. The release rate of the hydrogen can be calculated by recording the degradation soaking time and the volume of the collected hydrogen, so that the change condition of the degradation rate of the magnesium alloy along with the degradation soaking time is reflected, and the degradation rate in the degradation process of the magnesium alloy is obtained.
The principle of the method for measuring the degradation of the magnesium alloy is as follows:
magnesium alloy degradation is known to involve the following chemical reactions:
Mg+2H 2 O＝Mg 2+ +2OH - +H 2 ↑
mg→Mg 2+ +2e
2H 2 O+2e→H 2 ↑+2OH -
Mg 2+ +2OH - →Mg(OH) 2 ↓
according to the release of 1mol of hydrogen from 1mol of magnesium, each release of 1mol of hydrogen corresponds to degradation of 1.083mg of magnesium element, and thus the mass loss of the degradable magnesium alloy material can be calculated. Degradation rate: the degradation rate formula of the degradable magnesium alloy is as follows: c=kΔm/ρat, where C is the annual degradation rate of the degradable metal, mm/a; k is well known, 8.76X10 4 The method comprises the steps of carrying out a first treatment on the surface of the Δm is the mass lost by the sample, g; ρ is the density of the material, g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the A is the initial surface area of the sample in cm 2 The method comprises the steps of carrying out a first treatment on the surface of the t is soaking time, h.
The degradation rate v= (W) of the material was calculated by the weight loss of samples taken at different time nodes 0 -W 1 -W 2 )/AT；W 0 Mg is the mass of the sample before degradation; w (W) 1 Mg is the mass of the sample after degradation and degradation products are removed; w (W) 2 Correcting the weight loss of the sample after degradation products are removed and the blank sample of the same batch of materials, wherein mg is the weight loss; a is the surface area of the sample, cm 2 The method comprises the steps of carrying out a first treatment on the surface of the T is the sample soaking time, and h.
The PH and the volume of hydrogen gas released from the node and the weight loss of the material at various times recorded during the experiment are shown in the following table. The surface area of the medical magnesium alloy material experiment body is 233.6mm 2 The degradation soaking time is 21 days, and the degradation rate in the period can be calculated through the total hydrogen release amount of the surface area of the medical magnesium alloy material experiment in the following table. In addition, the degradation weight loss is also measured, and the soaking is carried out at the beginningThe degradation rate is very slow, and the degradation rate is rapidly accelerated and is slowed down in the later stage of soaking after the soaking time exceeds 48 hours. Therefore, the method can be used for monitoring the degradation process of the magnesium alloy, and is beneficial to researching the degradation mechanism of the magnesium alloy.
The degradation rate of the magnesium alloy material after 21 days is 3.51mm/y.
The degradation rate of the magnesium alloy material obtained by weight loss calculation in 21 days is 3.54mm/y.
The calculation result shows that the method of the invention verifies the reliability of the degradation performance of the medical magnesium alloy material.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention, and are intended to be included within the scope of the appended claims and description.
Claims (9)
1. The medical magnesium alloy material in-vitro degradation rate experimental device is characterized in that: the liquid-filled self-sealing device comprises an SBF simulated body fluid container (1) and a burette (6), wherein the upper part of the SBF simulated body fluid container (1) is provided with a sealing cover (2), and the lower part of the burette (6) penetrates through the sealing cover (2) and then stretches into the SBF simulated body fluid container (1); a cover body (5) for covering a medical magnesium alloy material experimental body is arranged at the bottom end of the burette (6), and a sealable hose (7) is arranged at the top end of the burette (6); volume graduation lines are arranged on the burette (6).
2. The medical magnesium alloy material in-vitro degradation rate experimental device according to claim 1, wherein: the SBF simulated body fluid container (1) is characterized in that a cylinder (4) for placing a medical magnesium alloy material experimental body is arranged at the bottom of the inside of the SBF simulated body fluid container (1), the top of the cylinder (4) is opened, and the opening position corresponds to the bottom end position of the burette (6).
3. The medical magnesium alloy material in-vitro degradation rate experimental device according to claim 2, wherein: a plurality of through liquid inlet holes (8) are formed in the cylinder body (4).
4. The medical magnesium alloy material in-vitro degradation rate experimental device according to claim 3, wherein: PH detection holes (3) which are convenient for PH detection are formed in the sealing cover (2), and corresponding plugs are matched with the sealing cover.
5. The medical magnesium alloy material in-vitro degradation rate experimental device according to claim 4, wherein: sealing rubber rings are arranged at the matching position of the sealing cover (2) and the SBF simulated body fluid container (1) and the matching position of the sealing cover (2) and the burette (6).
6. The medical magnesium alloy material in-vitro degradation rate experimental device according to claim 5, wherein: the cover body (5) is of an inverted funnel shape.
7. The in vitro degradation rate experimental method for the medical magnesium alloy material adopts the in vitro degradation rate experimental device for the medical magnesium alloy material according to the claims 1-6, and is characterized by comprising the following steps:
a. pouring SBF simulated body fluid into the SBF simulated body fluid container (1), placing a medical magnesium alloy material experimental body into the cylinder (4), then buckling an inverted funnel-shaped cover body (5) to cover the whole cylinder (4), and connecting a burette (6) at the tail end of the inverted funnel-shaped cover body (5);
b. the upper end of the burette (6) is connected with a sealable hose (7), the sealable hose (7) is connected with a vacuumizing device to vacuumize the interior of the burette (6) and suck SBF simulated body fluid to the highest volume scale of the burette (6), and then the sealable hose (7) is sealed;
c. standing the whole device, recording the scale change of SBF simulated body fluid level drop according to a preset time interval, obtaining the hydrogen generation amount of the medical magnesium alloy material experimental body, and then calculating the instantaneous degradation rate V and the average degradation rate C of the medical magnesium alloy material experimental body.
8. The method for testing the in-vitro degradation rate of the medical magnesium alloy material according to claim 7, wherein the method comprises the following steps: in the step c, the instantaneous degradation rate V= (W) 0 -W 1 -W 2 ) AT, wherein W 0 Mg is the mass of the medical magnesium alloy material experimental body before degradation; w (W) 1 Mg is the mass of the sample after degradation and degradation products are removed; w (W) 2 Correcting the weight loss of the blank sample of the medical magnesium alloy material experimental body after degradation products are removed from the medical magnesium alloy material experimental body and the medical magnesium alloy material experimental body in the same batch, wherein mg is the weight loss; a is the surface area of the sample, cm 2 The method comprises the steps of carrying out a first treatment on the surface of the T is the sample soaking time, and h.
9. The method for testing the in-vitro degradation rate of the medical magnesium alloy material according to claim 7, wherein the method comprises the following steps: in the step C, the average degradation rate C=K delta m/rho At of the medical magnesium alloy material experimental body is shown in the formula; k is well known, 8.76X10 4 The method comprises the steps of carrying out a first treatment on the surface of the Δm is the mass lost by the sample, g; ρ is the density of the material, g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the A is the initial surface area of the sample in cm 2 The method comprises the steps of carrying out a first treatment on the surface of the t is soaking time, h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310246741.7A CN116223720A (en) | 2023-03-10 | 2023-03-10 | Medical magnesium alloy material in-vitro degradation rate experimental device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310246741.7A CN116223720A (en) | 2023-03-10 | 2023-03-10 | Medical magnesium alloy material in-vitro degradation rate experimental device and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116223720A true CN116223720A (en) | 2023-06-06 |
Family
ID=86576748
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310246741.7A Pending CN116223720A (en) | 2023-03-10 | 2023-03-10 | Medical magnesium alloy material in-vitro degradation rate experimental device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116223720A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101968477A (en) * | 2010-08-27 | 2011-02-09 | 华南理工大学 | Medical integrated magnesium alloy biodegradability gas-collection measurement device |
| CN101968478A (en) * | 2010-08-27 | 2011-02-09 | 华南理工大学 | Equipment for dynamically simulating and testing biodegradability of medical magnesium alloy in vitro |
| CN102564932A (en) * | 2011-12-28 | 2012-07-11 | 天津商业大学 | Method for determining magnesium or magnesium alloy corrosion rate and special experimental device |
-
2023
- 2023-03-10 CN CN202310246741.7A patent/CN116223720A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101968477A (en) * | 2010-08-27 | 2011-02-09 | 华南理工大学 | Medical integrated magnesium alloy biodegradability gas-collection measurement device |
| CN101968478A (en) * | 2010-08-27 | 2011-02-09 | 华南理工大学 | Equipment for dynamically simulating and testing biodegradability of medical magnesium alloy in vitro |
| CN102564932A (en) * | 2011-12-28 | 2012-07-11 | 天津商业大学 | Method for determining magnesium or magnesium alloy corrosion rate and special experimental device |
Non-Patent Citations (2)
| Title |
|---|
| 胡骞 等: "AZ31镁合金在模拟体液中的腐蚀行为研究", 武汉科技大学学报, vol. 36, no. 5, 31 October 2013 (2013-10-31), pages 353 - 357 * |
| 赵虹等: "医用镁合金生物降解行为研究进展", 材料导报, vol. 27, no. 19, 31 October 2013 (2013-10-31), pages 78 - 85 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5833630A (en) | Sample collection device | |
| ITMI20020334V0 (en) | DEVICE FOR THE COLLECTION OF BLOOD SAMPLES TO BE SUBMITTED APPROVES FOR EXAMPLE THE RATE OF GLUCOSE CONTAINED IN IT | |
| CN108064149A (en) | Biological fluid collection device | |
| CA2428094A1 (en) | Method and apparatus for the measurement of intra-abdominal pressure | |
| CN108759733A (en) | With the device and method of gasmetry irregularly shaped object volume | |
| CN116223720A (en) | Medical magnesium alloy material in-vitro degradation rate experimental device and method | |
| CN110940613A (en) | Simulation experiment device for carbon isotope analysis in shale gas release process | |
| US4298011A (en) | Blood sample collector | |
| CN106175776A (en) | A kind of lymphedema limbs volume measurement device | |
| CN104990833B (en) | Gas absorbs volume detector and detection method | |
| CN206177638U (en) | Sampling device for medical testing | |
| KR100900655B1 (en) | Blood analyzing apparatus equipped with blood suction collection device maintaining regular collection volume | |
| CN204116098U (en) | A kind of novel urine sampler | |
| CN201173894Y (en) | Rubber pipette bulb type color comparison dish | |
| CN211434390U (en) | Urine collection bag for urinalysis | |
| CN101579234A (en) | Mercury-free type blood-pressure meter | |
| CN209695205U (en) | A kind of peripheral blood collecting tube of Anti-solidification | |
| CN209032452U (en) | One kind swallowing formula oesophagus sampler | |
| CN202982003U (en) | Testing device for lung capacity | |
| CN113358638A (en) | Portable saliva sampling detection device | |
| CN208636086U (en) | A kind of disposable saliva protection acquisition device | |
| JP3954898B2 (en) | Inspection container | |
| CN111855998B (en) | Sealed specimen processing device and application method thereof | |
| RU2325845C1 (en) | Helicobacter pylori infection non-invasive disease diagnosis | |
| CN214931770U (en) | Urine sample save set for urological department |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |