CN116211755A - White truffle essence with anti-wrinkle antioxidant skin barrier enhancing effects and preparation method thereof - Google Patents
White truffle essence with anti-wrinkle antioxidant skin barrier enhancing effects and preparation method thereof Download PDFInfo
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- CN116211755A CN116211755A CN202211601009.9A CN202211601009A CN116211755A CN 116211755 A CN116211755 A CN 116211755A CN 202211601009 A CN202211601009 A CN 202211601009A CN 116211755 A CN116211755 A CN 116211755A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/48—Thickener, Thickening system
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
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- Microbiology (AREA)
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- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to the technical field of skin care products, and discloses a white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier, which comprises the following components: white truffle extract, trehalose, allantoin, 140-kilo-molecular-weight sodium hyaluronate, EDTA-disodium, butanediol, phenoxyethanol and deionized water. The steps are as follows: weighing white truffle raw materials, adding deionized water for cleaning, and putting the white truffle raw materials into a small-sized pulverizer for pulverizing after cleaning; adding cellulase and pectase, heating in water bath, stirring for enzymolysis, heating in water bath, filtering with filter paper, and concentrating to obtain white truffle extract; proportioning according to a proportion, stirring and dissolving by using a stirrer to obtain a mixture; and D, filtering and sterilizing the mixed solution obtained in the step three by using a filter membrane, and bottling the filtrate obtained by filtering to obtain the finished product. Can effectively improve the effects of resisting wrinkle, resisting oxidation and enhancing skin barrier of skin.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier and a preparation method thereof.
Background
White truffle is a truffle mushroom and is known as truffle Tuber magnatum. Because of the precious nature, there is a name for white diamond. White truffle contains rich polysaccharide, amino acid and protein substances, and is pursued by broad food lovers due to unique smell and extremely low yield. Although the white truffle has a long edible history as a precious food material, the white truffle is applied to the daily chemical beauty industry by a few people, and the beauty effect of the white truffle on the skin of a human body is not yet explored and fully utilized by people. Although a small amount of white truffle skin care products appear in the market, most of the white truffle skin care products are conceptual products, and the skin care effect of the white truffle is not clarified and verified.
In document CN 115381743A, a composition with antioxidant and antiaging functions, and a preparation method and application thereof are disclosed, wherein the composition does not contain white truffle extract, has no effects of anti-wrinkle and antioxidant and enhancing skin barrier, and the composition is not very good when used.
Disclosure of Invention
In order to solve the problems, the invention discloses a white truffle essence with the effects of resisting wrinkles, resisting oxidation and enhancing skin barrier and a preparation method thereof, which can effectively improve the effects of resisting wrinkles, resisting oxidation and enhancing skin barrier of skin.
The technical scheme of the invention is as follows: white truffle essence with anti-wrinkle, antioxidant and skin barrier enhancing effects, the composition comprises the following components: white truffle extract, trehalose, allantoin, 140-kilo-molecular-weight sodium hyaluronate, EDTA-disodium, butanediol, phenoxyethanol and deionized water. The white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier comprises the following components in percentage by weight:
the preparation method of the white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier comprises the following steps:
step one: weighing white truffle raw materials, adding deionized water with the mass 1-2 times of that of white truffle, cleaning, and then putting into a small-sized pulverizer for pulverizing;
step two: sequentially adding cellulase and pectase, heating in water bath, stirring for enzymolysis, heating in water bath, filtering with filter paper plate, and concentrating to 1/2-1/3 of the filtrate to obtain white truffle extract;
step three: proportioning according to a proportion, stirring and dissolving by using a stirrer to obtain a mixture;
step four: and D, filtering and sterilizing the mixed solution obtained in the step three by using a filter membrane, and bottling the filtrate obtained by filtering to obtain the finished product.
Further, the pulverizer in the first step pulverizes the white truffle to 20-50 meshes.
Further, in the second step, the cellulose is 0.1-0.3% of the mass of the white truffle raw material, and the pectase is 0.1-0.3% of the mass of the white truffle raw material.
Further, in the second step, the white truffle is heated and stirred in a water bath at the temperature of 40-45 ℃ for 1-2 hours to carry out enzymolysis, and after the enzymolysis, the white truffle is heated in a water bath at the temperature of 80-90 ℃ for 3-5 hours, wherein the thickness of the filter paper board is 0.9-1.1 mu m, and the concentration temperature is 70-80 ℃.
Further, in the third step, the stirring speed of the stirrer is 100-200r/min, and the stirring time is 0.5-1 h.
Further, the filter membrane in the fourth step is 0.44-0.46 μm.
The invention has the advantages that: 1. the white truffle extract has the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier, and is applied to essence, so that the essence has the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier, and then the moisturizing effect of the essence is enhanced by adding trehalose and sodium hyaluronate.
2. The sodium hyaluronate with large molecular weight used in the invention can play a role of a thickener, thereby avoiding the damage to the skin and the poor use feeling of the common chemical thickener and improving the experience of users.
3. The white truffle essence used in the invention can effectively play roles of resisting wrinkles, resisting oxidization and enhancing skin barrier, is safe and efficient, meets the requirements of people on cosmetic safety, and has wide application prospect.
Drawings
FIG. 1 is a cytotoxicity bar graph of example 1;
FIG. 2 is a histogram of anti-wrinkle-associated gene expression in example 1;
FIG. 3 is a bar graph of cytotoxicity evaluation of example 2;
FIG. 4 is a reactive oxygen species assay ROS assay histogram of example 2;
FIG. 5 is a bar graph showing SPT results of the skin barrier related genetic expression test of example 3;
FIG. 6 is a bar graph showing the results of the skin barrier related gene expression test ABCA12 of example 3.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Example 1
The white truffle essence for resisting wrinkle, resisting oxidation and enhancing skin barrier comprises the following components in percentage by weight: 1% of white truffle extract, 2% of trehalose, 0.1% of allantoin, 0.1% of sodium hyaluronate with 140 ten thousandth molecular weight, 0.01% of EDTA-disodium, 5% of butanediol, 0.3% of phenoxyethanol and 91.49% of deionized water. This example is prepared by the steps of:
(1) Weighing white truffle raw materials, adding deionized water with the mass 1 time of that of white truffle, cleaning, and then putting into a small-sized pulverizer for pulverizing to 20 meshes; sequentially adding cellulase accounting for 0.3% of the mass of the white truffle raw material and pectase accounting for 0.3% of the mass of the white truffle raw material, heating in a water bath at 45 ℃ and stirring for 1h for enzymolysis, heating in a water bath at 90 ℃ for 5h, filtering by a1 mu m filter paper plate, and concentrating at 70 ℃ to 1/3 of the mass of the filtrate to obtain white truffle extract;
(2) Mixing according to the proportion, and stirring and dissolving for 1h by a 200r/min stirrer;
(3) Filtering with 0.45 μm filter membrane, sterilizing, and bottling the filtrate to obtain the final product.
Example 2
The white truffle essence for resisting wrinkle, resisting oxidation and enhancing skin barrier comprises the following components in percentage by weight: 5% of white truffle extract, 2% of trehalose, 0.2% of allantoin, 0.5% of sodium hyaluronate with 140 ten thousandth molecular weight, 0.02% of EDTA-disodium, 5% of butanediol, 0.5% of phenoxyethanol and 86.78% of deionized water. This example is prepared by the steps of:
(1) Weighing white truffle raw materials, adding deionized water with the mass 1 time of that of white truffle, cleaning, and then putting into a small-sized pulverizer for pulverizing to 20 meshes; sequentially adding cellulase accounting for 0.3% of the mass of the white truffle raw material and pectase accounting for 0.3% of the mass of the white truffle raw material, heating in a water bath at 45 ℃ and stirring for 1h for enzymolysis, heating in a water bath at 90 ℃ for 5h, filtering by a1 mu m filter paper plate, and concentrating at 70 ℃ to 1/3 of the mass of the filtrate to obtain white truffle extract;
(2) Mixing according to the proportion, and stirring and dissolving for 1h by a 200r/min stirrer;
(3) Filtering with 0.45 μm filter membrane, sterilizing, and bottling the filtrate to obtain the final product.
Example 3
The white truffle essence for resisting wrinkle, resisting oxidation and enhancing skin barrier comprises the following components in percentage by weight: 5% of white truffle extract, 5% of trehalose, 0.5% of allantoin, 0.5% of sodium hyaluronate with 140 ten thousandth molecular weight, 0.05% of EDTA-disodium, 10% of butanediol, 0.7% of phenoxyethanol and 78.25% of deionized water. This example is prepared by the steps of:
(1) Weighing white truffle raw materials, adding deionized water with the mass of 2 times that of white truffle, cleaning, and then putting into a small-sized pulverizer for pulverizing to 20 meshes; sequentially adding cellulase accounting for 0.3% of the mass of the white truffle raw material and pectase accounting for 0.3% of the mass of the white truffle raw material, heating in a water bath at 45 ℃ and stirring for 1h for enzymolysis, heating in a water bath at 90 ℃ for 5h, filtering by a1 mu m filter paper plate, and concentrating at 70 ℃ to 1/3 of the mass of the filtrate to obtain white truffle extract;
(2) Mixing according to the proportion, and stirring and dissolving for 1h by a 200r/min stirrer;
(3) Filtering with 0.45 μm filter membrane, sterilizing, and bottling the filtrate to obtain the final product.
According to the invention, the white truffle is prepared into the white truffle extract, and a series of efficacy verification experiments prove that the white truffle extract has the effects of resisting wrinkles and oxidization and enhancing skin barrier, and is applied to the essence, so that the essence has the effects of resisting wrinkles and oxidization and enhancing skin barrier, and then the moisturizing effect of the essence is enhanced by adding trehalose and sodium hyaluronate, and the sodium hyaluronate with high molecular weight can play a role of a thickening agent, so that the damage of a common chemical thickening agent to skin and poor use feeling are avoided.
In order to better illustrate the beneficial effects of the invention, efficacy evaluation experiments were performed:
experimental example 1:
anti-wrinkle efficacy cell experiment of white truffle extract
1. Purpose of experiment
This study was conducted to evaluate the anti-wrinkle efficacy of the white truffle extract.
2. Experimental method
1) Cytotoxicity evaluation
After 24 hours of cell culture on 24-well plates, the cells were inoculated and replaced with serum-free medium, and the cells were treated at different concentrations and cultured. After the completion of the culture, the cells were replaced with a medium containing an MTT solution, the reaction was performed, the supernatant was removed after the completion of the reaction, and MTT formazan crystals produced by dissolving dimethyl sulfoxide (DMSO) were added thereto, and then the absorbance was measured at a wavelength of 570nm using an enzyme-labeled instrument.
2) Anti-wrinkle related genetic gene expression evaluation method
Inoculating human fibroblasts in a 60mm dish, culturing, washing with buffer solution, irradiating with UVA, changing to serum-free culture medium, treating sample, and culturing at 37deg.C with 5% CO 2 Culturing in a microorganism incubator using QIAzol TM The lysis reagent collects cells and RNA is isolated according to the manufacturer's method. After the RNA is quantified after the separation, cDNA is synthesized for Real-timePCR (Applied Biosystems kit, rapid real-time quantitative PCR apparatus).
3. Experimental results
1) Cytotoxicity (MTT assay)
Note that: control is a blank group, cell Viability (%) is Cell activity (%) white truffle extract was not cytotoxic to 0.05% concentration, and the maximum concentration of this experiment was performed to 0.05%.
2) Anti-wrinkle related genetic gene expression
Note that: control is blank, UVA25J/cm 3 For UVA irradiation, UVA+EGCG is UVA+epigallocatechin gallate, MMP-1gene expression (%) is MMP-1gene expression rate
The white truffle extract was found to have an inhibitory effect on MMP-1 expression at a concentration of 0.005%,0.01%, 0.025%.
4. Conclusion(s)
Ultraviolet (UVA) decomposes matrix components such as cells of the dermis layer of the skin and elastin by specifically decomposing the main matrix components of dermis, collagen I, III, V and regulating the synthesis of various MMPs of the main matrix components of basement membrane, collagen IV, VII, proteolycans, laminin, etc., to induce the generation of skin wrinkles and photoaging.
Therefore, when the addition amount of the white truffle extract is more than or equal to 0.005%, the white truffle extract has excellent anti-wrinkle effect by preventing the activity of MMP-1 which causes skin wrinkles and photoaging in human skin fibroblasts irradiated by ultraviolet rays.
The content of the white truffle extract is 1% -5%, and is far greater than 0.005%, so that the white truffle extract has excellent anti-wrinkle effect.
Experimental example 2:
cell experiment of antioxidation efficacy of white truffle extract
1. Purpose of experiment
This study was conducted to evaluate the antioxidant efficacy of the white truffle extract.
2. Experimental method
3) Cytotoxicity evaluation (MTT assay)
After 24 hours of cell culture on 24-well plates, the cells were inoculated and replaced with serum-free medium, and the cells were treated at different concentrations and cultured. After the completion of the culture, the cells were replaced with a medium containing an MTT solution, the reaction was performed, the supernatant was removed after the completion of the reaction, and MTT formazan crystals produced by dissolving dimethyl sulfoxide (DMSO) were added thereto, and then the absorbance was measured at a wavelength of 570nm using an enzyme-labeled instrument.
4) Reactive oxygen assay ROS assay (dichlorofluorescein acetoacetate staining DCFDA)
staining)
Human-derived keratinocyte cell line (Human keratinocyte cells) was aliquoted on 96-well plates at 37℃with 5% CO 2 After the microorganism culture vessel is stable, the sample is treated with a serum-free medium and then cultured. After washing with buffer solution, H is added 2 DcFDA (dichlorofluorescein diacetate) solution followed by 5% CO at 37deg.C 2 The reaction is carried out in a microorganism incubator. Washing with buffer solution, adding H 2 O 2 After the solution was heated to 37℃and 5% CO 2 The reaction is carried out in a microorganism incubator. Fluorescence values (fluorescence value) (Ex) were determined after buffer washes.
485/em.530). After Buffer removal, triton-X100 was added, BCA (bis-quinolinecarboxylic acid) solution at 37℃with 5% CO 2 The reaction in the microorganism incubator was measured for the protein expression amount by measuring absorbance (562 nm).
3. Experimental results
3) Cytotoxicity evaluation (MTT assay)
Note that: control is a blank group, cell Viability (%) is Cell activity (%)
-white truffle extract was non-cytotoxic to 0.1% concentration, the maximum concentration of this experiment was performed to 0.1%.
4) Reactive oxygen species assay ROS assay
Note that: ROS production (%) is the reaction oxygen production (%)
The white truffle extract was confirmed to have an effect of blocking ROS reactive oxygen species formation at 0.01% concentration.
4. Conclusion(s)
Reactive oxygen species (reactive oxygen species, ROS) have been studied as one of the major causes of skin aging, and various diseases are produced by damaging nucleic acid substances in cells or by causing cell death by triggering lipid peroxidation of cell membranes or the like. Through the experiment, the white truffle extract has the effect of resisting skin oxidation by preventing ROS (reactive oxygen species) generation caused by hydrogen peroxide when the addition amount is more than or equal to 0.01 percent. Accordingly, white truffle extract is recommended as a raw material for protecting skin from oxidation damage and having antioxidant and anti-aging effects.
The content of the white truffle extract is 1% -5%, and is far more than 0.01%, so that the white truffle extract has excellent antioxidation effect.
Experimental example 3:
skin barrier enhancing efficacy cell experiment of white truffle extract
1. Purpose of experiment
The skin barrier strengthening efficacy of the white truffle extract was tested.
2. Experimental method
1) Skin barrier related genetic gene expression experiment method comprises inoculating human normal keratinocyte (Human normal keratinocyt) in a 60mm dish for culturing in real-time quantitative PCR (Real time PCR), changing to serum-free medium, treating sample at different concentrations, and culturing in 3
7℃,5％ CO 2 After removal of the medium, QIAz was used
ol TM The lysis reagent collects cells and RNA is extracted according to the manufacturer's method. Quantification of RNA after extraction and synthesis of cDNAThe cDNA was subjected to Real-time PCR (Applied Biosystems kit, rapid Real-time quantitative PCR apparatus) to confirm the genetic gene expression (SPT,
ABCA-12)。
3. experimental results
1) Skin barrier related genetic gene expression experiments: SPT (SPT)
Note that: control is blank, retinoic acid is Retinoic acid, SPT gene
expression (%) is the SPT gene expression effect.
The white truffle extract was confirmed to have an effect of increasing the expression of the SPT gene at the full concentration.
2) Skin barrier related genetic gene expression experiments: ABCA12
Note that: control is blank, retinoic acid is Retinoic acid, ABCA12
gene expression (%) is the ABCA12 gene expression effect.
3) The white truffle extract has the effect of increasing the expression of ABCA12 genes under the condition of full concentration.
4. Conclusion(s)
SPT (serin palmitoyl transferase) interfering with ceramide biosynthesis is an important role in maintaining skin homeostasis, and ABCA12 (ATP-binding cassette transporter 12) can interfere with lipid metabolism, cholesterol release and movement, and has an important role in skin lipid formation.
The experiment proves that the white truffle extract can achieve the excellent effect of enhancing the skin barrier by increasing the expression of SPT and ABCA12 genetic genes under all concentrations of the experiment. The content of the white truffle extract is 1% -5%, so that the white truffle extract has an excellent effect of enhancing skin barrier.
It will be appreciated by persons skilled in the art that the embodiments of the invention described above and shown in the drawings are by way of example only and not limitation, and that the objects of the invention have been fully and effectively achieved. The functional and structural principles of the present invention have been shown and described in the examples and embodiments of the invention may be modified or practiced without departing from the principles described.
Claims (7)
1. The white truffle essence with the effects of resisting wrinkle, resisting oxidation and enhancing skin barrier is characterized in that,
comprises the following components in percentage by weight:
1% -5% of white truffle extract;
2% -5% of trehalose;
allantoin 0.1% -0.5%;
sodium hyaluronate with 140 ten thousand molecular weight 0.1% -0.5%;
EDTA-disodium 0.01% -0.05%;
5% -10% of butanediol;
0.3% -0.7% of phenoxyethanol;
78.25% -91.49% of deionized water.
2. A process for preparing a white truffle concentrate with anti-wrinkle antioxidant skin barrier enhancing effect according to claim 1, characterized by comprising the steps of:
step one: weighing white truffle raw materials, adding deionized water with the mass 1-2 times of that of white truffle, cleaning, and then putting into a small-sized pulverizer for pulverizing;
step two: sequentially adding cellulase and pectase, heating in water bath, stirring for enzymolysis, heating in water bath, filtering with filter paper plate, and concentrating to 1/2-1/3 of the filtrate to obtain white truffle extract;
step three: proportioning according to the component proportion of the white truffle essence, and stirring and dissolving by a stirrer to obtain a mixture;
step four: and D, filtering and sterilizing the mixed solution obtained in the step three by using a filter membrane, and bottling the filtrate obtained by filtering to obtain the finished product.
3. The preparation method of the white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier according to claim 2, which is characterized in that: and (3) crushing the white truffle to 20-50 meshes by using a crusher in the step one.
4. The preparation method of the white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier according to claim 2, which is characterized in that: in the second step, the cellulose is 0.1-0.3% of the mass of the white truffle raw material, and the pectase is 0.1-0.3% of the mass of the white truffle raw material.
5. The preparation method of the white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier according to claim 2, which is characterized in that: in the second step, the white truffle is heated and stirred in a water bath at 40-45 ℃ for 1-2 hours for enzymolysis, and after the enzymolysis, the white truffle is heated in a water bath at 80-90 ℃ for 3-5 hours, wherein the thickness of the filter paper board is 0.9-1.1 mu m, and the concentration temperature is 70-80 ℃.
6. The preparation method of the white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier according to claim 2, which is characterized in that: and in the third step, the stirring speed of the stirrer is 100-200r/min, and the stirring time is 0.5-1 h.
7. The preparation method of the white truffle essence with the effects of resisting wrinkles, resisting oxidization and enhancing skin barrier according to claim 2, which is characterized in that: the filter membrane in the fourth step is 0.44-0.46 μm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104666237A (en) * | 2015-02-12 | 2015-06-03 | 广州悦目生物科技有限公司 | Preparation method and application of truffle active ingredient |
| CN107961191A (en) * | 2017-12-28 | 2018-04-27 | 广州蜜妆生物科技有限公司 | A kind of kahikatea dew anti-aging is compacted eye mask and preparation method thereof |
| CN110022850A (en) * | 2016-11-29 | 2019-07-16 | 莱雅公司 | Composition comprising truffle extract and neohesperidin dihydrochalcone |
| CN115368481A (en) * | 2022-08-08 | 2022-11-22 | 北京工商大学 | Preparation method and application of multifunctional truffle polysaccharide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666237A (en) * | 2015-02-12 | 2015-06-03 | 广州悦目生物科技有限公司 | Preparation method and application of truffle active ingredient |
| CN110022850A (en) * | 2016-11-29 | 2019-07-16 | 莱雅公司 | Composition comprising truffle extract and neohesperidin dihydrochalcone |
| CN107961191A (en) * | 2017-12-28 | 2018-04-27 | 广州蜜妆生物科技有限公司 | A kind of kahikatea dew anti-aging is compacted eye mask and preparation method thereof |
| CN115368481A (en) * | 2022-08-08 | 2022-11-22 | 北京工商大学 | Preparation method and application of multifunctional truffle polysaccharide |
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