CN116200248B - High-precision manual embryo picking device - Google Patents

High-precision manual embryo picking device Download PDF

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Publication number
CN116200248B
CN116200248B CN202310101259.4A CN202310101259A CN116200248B CN 116200248 B CN116200248 B CN 116200248B CN 202310101259 A CN202310101259 A CN 202310101259A CN 116200248 B CN116200248 B CN 116200248B
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sliding
embryo
shell
threaded rod
screw
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CN202310101259.4A
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CN116200248A (en
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张明
王之盛
易军
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Sichuan Agricultural University
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Sichuan Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a high-precision manual embryo picking device which comprises a main shell, an upper shell and a lower shell, wherein the upper shell and the lower shell are arranged at the upper end and the lower end of the main shell; the side face of the main shell is provided with a plurality of sliding grooves, the sliding grooves are internally provided with the sliding shell, the outer side end and the inner side end of the sliding shell are respectively provided with a poking wheel and a diagonal gear which is used for being in rotary fit with the diagonal screw, and the poking wheel is rotationally connected with the diagonal gear through a reduction gear set; compared with the traditional mouth suction embryo (egg) picking tube, the scheme has the advantages of convenient operation, higher precision and capability of effectively avoiding pollution.

Description

High-precision manual embryo picking device
Technical Field
The invention relates to the technical field of life science embryo engineering, in particular to a high-precision manual embryo picking device.
Background
Under the drawing action of an alcohol lamp, a glass tube with 3-5 mm for a traditional mouth suction embryo (egg) picking tube leads the tube diameter of the front end of the glass tube to be 200 microns, then the rear end of the glass tube is connected with a plastic hose with elasticity, and the tail end of the plastic hose is connected in the mouth of an operator and plugs the hose; the right hand of an operator controls the entering speed and the discharging speed of liquid at the proximal end of the glass tube by pressing the plastic hose so as to sort and inspect the oocytes or embryos in the liquid, but the embryo (egg) picking mode has the following defects:
1. the original embryo (egg) picking tube can only be manually drawn on the flame of the alcohol lamp, so that large-scale batch production is difficult to realize, and a front-stage capillary tube drawn by glass is easy to break in embryo (egg) picking operation;
2. the traditional mouth suction embryo (egg) picking tube relies on the coordination control of the hands and the mouths of operators to realize embryo (egg) picking operation, and the operators can control the liquid flow of capillary siphonage at the front section of the capillary glass tube well after obtaining good experience in a large amount of exercises, and the operation precision is lower;
3. the mouth aspiration embryo (egg) picking tube is easy to form cavity microorganisms, thereby causing embryo pollution.
Therefore, there is an urgent need for an embryo picking device that is convenient to operate, has higher operation precision, and can avoid contamination.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a high-precision manual embryo picking device, which solves the problems of high embryo picking operation difficulty and low precision in the prior art.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the high-precision manual embryo picking device comprises a main shell, an upper shell and a lower shell, wherein the upper shell and the lower shell are arranged at the upper end and the lower end of the main shell, movable diagonal screws are arranged in the main shell, threaded rods parallel to the diagonal screws are arranged at the two ends of the diagonal screws, fixing nuts which are respectively in threaded fit with the two threaded rods are arranged in the upper shell and the lower shell, a piston rod parallel to the threaded rods is arranged at the end part of the threaded rod in the lower shell, a piston cylinder is arranged in the lower shell, the piston rod is in sliding sealing fit with a piston cavity in the piston cylinder, an embryo picking needle fixing device is arranged at the lower end of the piston cylinder, and an inner cavity of the embryo picking needle is communicated with the piston cavity; the side of main casing is provided with a plurality of sliding grooves, and the twill screw rod wears to establish simultaneously in a plurality of sliding grooves, and the sliding motion of sliding groove is provided with the sliding casing, and the outside end and the inboard end of sliding casing are provided with respectively and dial the wheel and are used for with twill screw rod normal running fit's twill gear, and stir wheel and twill gear and bulge the outside end and the inboard end of sliding casing respectively, stir the wheel and pass through reduction gear set and twill gear rotation connection, the reduction ratio of the reduction gear set in a plurality of sliding casings is all inequality.
The beneficial effects of adopting above-mentioned technical scheme are: according to the scheme, the poking wheel at the outer side end of the sliding shell is manually pressed, the sliding shell can slide inwards along the sliding groove, the poking wheel is poked by fingers, the poking wheel can be driven to rotate, and as the threaded rods at the two ends of the poking screw are matched with the threads of the fixing nuts, the poking screw can axially move while rotating, so that the piston rod is driven to axially move in the piston cavity, and the embryo picking device is convenient to operate and high in reliability compared with the traditional mouth embryo picking (egg) tube, and is low in dependence on the operation experience of workers; the speed of the diagonal screw can be reduced by times through the reduction gear set, and meanwhile, the multiple gears of the speed of the diagonal screw are adjusted through the reduction gear sets with different reduction ratios by the sliding shells, so that the movement stroke of the piston rod is controlled accurately, the emptying volume is controlled accurately when the piston rod moves, the emptying volume is matched with the embryo or cell volume, and the operation precision when the embryo or cell is sucked is improved.
Further, a baffle plate is arranged in the sliding groove, an abutting groove is formed in the inner side end of the sliding shell, a reset spring is arranged between the baffle plate and the abutting groove, and two ends of the reset spring are respectively abutted with the bottom of the abutting groove and the baffle plate.
The beneficial effects of adopting above-mentioned technical scheme are: when the user manually presses the poking wheel to enable the diagonal gear to be meshed with the diagonal screw, the reset spring is compressed, and after the poking wheel is not pressed, the diagonal gear and the diagonal screw can be separated under the action of tension of the reset spring, so that reset of the sliding shell is achieved.
Further, the opening of a plurality of sliding grooves is provided with the side cover plate that is used for spacing a plurality of sliding housing, prevents that sliding housing from sliding out from the sliding groove, is provided with a plurality of mouthfuls of dodging that are used for dodging the wheel on the side cover plate, and convenient to use person stirs the wheel through the finger to stirring.
Further, the piston rod is arranged on the threaded rod in a freely rotatable manner through the connector, the connector comprises a sleeve fixedly connected with the end part of the threaded rod and a joint fixedly connected with the end part of the piston rod, a bearing is arranged in the sleeve, and the joint is rotationally connected with the sleeve through the bearing.
Further, at least three sliding shells are arranged on the main shell, and the reduction ratios of the reduction gear sets in the three sliding shells are respectively 10: 1. 5:1 and 2:1. the axial length of each circle of threads on the diagonal screw rod is 0.25mm, the diameter of the piston rod is 1mm, and the diameter of the inner cavity of the embryo picking needle is 250 mu m.
Further, the radial dimension of the diagonal screw is larger than that of the threaded rod, so that the diagonal screw can only move between the two fixing nuts matched with the threaded rod, the axial movement stroke of the diagonal screw and the piston rod is limited, and tripping between the diagonal screw and the diagonal gear is effectively prevented.
Further, an avoidance cavity for avoiding the threaded rod is formed in the upper shell.
The beneficial effects of the invention are as follows:
the scheme is mainly used for embryo engineering or cell engineering, and is used for sorting cells, oocytes and embryos with the diameter of 10-220 micrometers; compared with the traditional mouth suction embryo (egg) picking tube, the mouth suction embryo (egg) picking tube has the advantages that the operation is convenient, the operation precision is higher, the siphoning and the discharging speed of the embryo picking tube for liquid are precisely controlled, the embryo (egg) picking operation is realized, and the contact between the mouth gas of an operator and the embryo liquid is avoided in the operation, so that the pollution is avoided.
Drawings
FIG. 1 is a schematic diagram of a high-precision manual embryo picking device.
FIG. 2 is an exploded view of a high precision manual embryo picker.
FIG. 3 is a cross-sectional view of a high precision manual embryo picker.
Fig. 4 is an exploded view of the connector.
Fig. 5 is a cross-sectional view of the connector.
Fig. 6 is an exploded view of the slide housing.
Fig. 7 is a schematic structural view of the sliding housing.
Wherein, 1, a main shell, 2, an upper shell, 3, a lower shell, 4, a diagonal screw, 5, a threaded rod, 6, a fixed nut, 7, a piston rod, 8, a piston cylinder, 9, a piston cavity, 10, a embryo picking needle fixing device, 11, a sliding groove, 12, a sliding shell, 13 and a poking wheel, 14, a diagonal gear, 15, a reduction gear set, 16, a baffle plate, 17, an abutting groove, 18, a return spring, 19, a side cover plate, 20, an avoidance opening, 21, a connector, 22, a sleeve, 23, a joint, 24, a bearing, 25, an avoidance cavity, 26 and an embryo picking needle.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and all the inventions which make use of the inventive concept are protected by the spirit and scope of the present invention as defined and defined in the appended claims to those skilled in the art.
As shown in fig. 1 to 3, the high-precision manual embryo picking device in the scheme comprises a main casing 1, an upper casing 2 and a lower casing 3, wherein the upper casing 2 and the lower casing 3 are arranged at the upper end and the lower end of the main casing 1, a movable diagonal screw 4 is arranged in the main casing 1, threaded rods 5 parallel to the diagonal screw 4 are arranged at the two ends of the diagonal screw 4, fixing nuts 6 which are respectively in threaded fit with the two threaded rods 5 are arranged in the upper casing 2 and the lower casing 3, an avoidance cavity 25 for avoiding the threaded rods 5 is arranged in the upper casing 2, a piston rod 7 parallel to the threaded rods 5 is arranged at the end of the threaded rods 5 in the lower casing 3, and the piston rod 7 is arranged on the threaded rods 5 in a freely rotatable manner through connectors 21.
The lower shell 3 is internally provided with a piston cylinder 8, the piston cylinder 8 and a piston cavity 9 inside the piston cylinder 8 are cylindrical, a piston rod 7 is in sliding sealing fit with the piston cavity 9 in the piston cylinder 8, so that the axial movement of the piston rod 7 can realize the suction and the evacuation of the piston cavity 9, the lower end of the piston cylinder 8 is provided with an embryo picking needle fixing device 10, an embryo picking needle 26 is arranged in the embryo picking needle fixing device 10, the inner cavity of the embryo picking needle 26 is communicated with the piston cavity 9, and the embryo picking needle 26 adopts standard 0.25mL transparent plastic tubule (bovine frozen semen preservation tubule) for drawing production, thereby realizing standardized and batch production.
As shown in fig. 4 and 5, the connector 21 comprises a sleeve 22 in threaded connection with the end of the threaded rod 5 and a joint 23 fixedly connected with the end of the piston rod 7, a bearing 24 is arranged in the sleeve 22, and the joint 23 is rotatably connected with the sleeve 22 through the bearing 24, wherein the bearing 24 adopts a bearing capable of bearing a certain axial load.
As shown in fig. 2, fig. 3, fig. 6 and fig. 7, three sliding grooves 11 are arranged side by side on the side surface of the main casing 1, the diagonal screw 4 is simultaneously penetrated in the three sliding grooves 11, the sliding casings 12 are slidably arranged in the three sliding grooves 11, the outer side end and the inner side end of the sliding casings 12 are respectively provided with a poking wheel 13 and a diagonal gear 14 used for being in running fit with the diagonal screw 4, the poking wheel 13 and the diagonal gear 14 respectively protrude out of the outer side end and the inner side end of the sliding casings 12, the poking wheel 13 is in running connection with the diagonal gear 14 through a reduction gear set 15, the reduction gear set 15 is realized by sequential transmission fit of a plurality of gears with different sizes, the reduction gear sets 15 in the three sliding casings 12 are combined through different gears to realize different reduction ratios, and the reduction ratios of the three reduction gear sets 15 are respectively 10: 1. 5:1 and 2:1, the rotation speed of the diagonal screw 4 can be reduced in multiple times through the reduction gear set 15, and meanwhile, the three sliding shells 12 realize multi-gear adjustment of the rotation speed of the diagonal screw 4 through the reduction gear sets 15 with different reduction ratios.
A baffle 16 is arranged in the sliding groove 11, an abutting groove 17 is arranged at the inner side end of the sliding shell 12, a return spring 18 is arranged between the baffle 16 and the abutting groove 17, two ends of the return spring 18 are respectively abutted against the bottom of the abutting groove 17 and the baffle 16, a side cover plate 19 for limiting three sliding shells 12 is arranged at the opening of the sliding groove 11, the sliding shells 12 are prevented from sliding out of the sliding groove 11, and three avoidance openings 20 for avoiding the poking wheels 13 are arranged on the side cover plate 19; when the user manually presses the dial wheel 13, the slide housing 12 can slide inward along the slide groove 11, so that the diagonal gear 14 is engaged with the diagonal screw 4, the return spring 18 is compressed, and after the dial wheel 13 is not pressed, the diagonal gear 14 is separated from the diagonal screw 4 by the tension of the return spring 18, so that the slide housing 12 is reset.
The radial dimension of the diagonal screw 4 is larger than that of the threaded rod 5, so that the diagonal screw 4 can only move between the two fixing nuts 6 matched with the threaded rod 5, the axial movement stroke of the diagonal screw 4 and the piston rod 7 is limited, and tripping between the diagonal screw 4 and the diagonal gear 14 is effectively prevented.
In specific implementation, a user manually presses the poking wheel 13 at the outer side end of the sliding casing 12, the twill gear 14 at the inner side end of the sliding casing 12 is meshed with the twill screw 4, the poking wheel 13 is poked by fingers, under the transmission action of the reduction gear set 15 and the twill gear 14, the twill screw 4 is driven to rotate, and as the threaded rods 5 at the two ends of the twill screw 4 are matched with the threads of the fixing nuts 6, the twill screw 4 can axially move while rotating, so that the piston rod 7 is driven to axially move in the piston cavity 9, and the embryo picking needle 26 fixed by the embryo picking needle fixing device 10 is used for sucking and discharging embryos or oocytes in liquid.
Compared with the traditional mouth suction embryo (egg) picking tube, the mouth suction embryo picking tube has the advantages of convenient operation, strong reliability, higher operation precision and low dependence on the operation experience of staff; the reduction gear sets 15 with different reduction ratios realize multi-gear adjustment of embryo or cell suction speed and discharge speed, and are beneficial to precisely controlling the movement stroke of the piston rod 7 while meeting the actual operation requirement, so that the evacuation volume during the movement of the piston rod 7 is precisely controlled, the evacuation volume is matched with the embryo or cell volume, and the operation precision during the embryo or cell suction and discharge is further improved.
Preferably, the axial length of each circle of threads on the diagonal screw 4 is 0.25mm, the diameter of the piston rod 7 is 1mm, and the diameter of the inner cavity of the embryo picking needle 26 is 250 μm, so that the stirring reduction ratio is 10: when the poking wheel 13 on the speed reducing gear set 15 of the motor 1 rotates 1/4 of the circle, the diagonal screw 4 rotates 1/40 of the circle, and the emptying volume of the piston cavity 9 is 4.9 multiplied by 10 6 μm 3 Moving the liquid in the inner cavity of the embryo picking needle 26 by 100 μm; similarly, the toggle reduction ratio is 5:1, when the stirring wheel 131/4 circles are arranged on the speed reducing gear set 15, the liquid in the inner cavity of the embryo picking needle 26 moves by 200 mu m; the toggle speed reduction ratio is 2:1, when the stirring wheel 131/4 circle is arranged on the speed reducing gear set 15, the inner cavity liquid of the embryo picking needle 26 is moved by 500 mu m, thereby facilitating the sorting of cells, oocytes and embryos with the diameter of 100-200 mu m.

Claims (4)

1. The utility model provides a manual embryo picking device of high accuracy, its characterized in that includes main casing (1) and upper casing (2) and lower casing (3) of setting up in the upper and lower both ends of main casing (1), be provided with movable diagonal screw (4) in main casing (1), the both ends of diagonal screw (4) all are provided with threaded rod (5) parallel with diagonal screw (4), be provided with respectively with two threaded rod (5) screw thread complex fixation nut (6) in upper casing (2) and the lower casing (3), be located the tip of threaded rod (5) in lower casing (3) be provided with threaded rod (7) parallel with threaded rod (5), be provided with piston cylinder (8) in lower casing (3), piston rod (7) and piston chamber (9) sliding seal cooperation in piston cylinder (8), the lower extreme of piston cylinder (8) is provided with and picks up embryo needle fixing device (10), be provided with embryo needle (26) in picking up embryo needle fixing device (10), the tip of picking up needle (26) communicates with piston chamber (9);
the side of the main shell (1) is provided with a plurality of sliding grooves (11), the cross grain screw (4) is simultaneously arranged in the sliding grooves (11) in a penetrating way, the sliding grooves (11) are internally provided with a sliding shell (12), the outer side end and the inner side end of the sliding shell (12) are respectively provided with a poking wheel (13) and a cross grain gear (14) which is used for being in running fit with the cross grain screw (4), the poking wheel (13) and the cross grain gear (14) respectively protrude out of the outer side end and the inner side end of the sliding shell (12), the poking wheel (13) is in rotary connection with the cross grain gear (14) through a reduction gear set (15), and the reduction ratios of the reduction gear sets (15) in the plurality of sliding shells (12) are different;
a baffle plate (16) is arranged in the sliding groove (11), an abutting groove (17) is formed in the inner side end of the sliding shell (12), a return spring (18) is arranged between the baffle plate (16) and the abutting groove (17), and two ends of the return spring (18) are respectively abutted against the bottom of the abutting groove (17) and the baffle plate (16); a side cover plate (19) for limiting the sliding shells (12) is arranged at the openings of the sliding grooves (11), and a plurality of avoiding openings (20) for avoiding the shifting wheels (13) are arranged on the side cover plate (19);
at least three sliding shells (12) are arranged on the main shell (1), and the reduction ratios of the reduction gear sets (15) in the three sliding shells (12) are respectively 10: 1. 5:1 and 2:1, a step of; the axial length of each circle of threads on the diagonal screw rod (4) is 0.25mm, the diameter of the piston rod (7) is 1mm, and the diameter of the inner cavity of the embryo picking needle (26) is 250 mu m.
2. The high-precision manual embryo picking device according to claim 1, wherein the piston rod (7) is rotatably arranged on the threaded rod (5) through a connector (21), the connector (21) comprises a sleeve (22) fixedly connected with the end of the threaded rod (5) and a joint (23) fixedly connected with the end of the piston rod (7), a bearing (24) is arranged in the sleeve (22), and the joint (23) is rotatably connected with the sleeve (22) through the bearing (24).
3. The high precision manual embryo picker according to claim 1, characterized in that the radial dimension of the diagonal screw (4) is greater than the radial dimension of the threaded rod (5).
4. The high-precision manual embryo picking device according to claim 1, wherein an avoidance cavity (25) for avoiding the threaded rod (5) is arranged in the upper shell (2).
CN202310101259.4A 2023-01-29 2023-01-29 High-precision manual embryo picking device Active CN116200248B (en)

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CN202310101259.4A CN116200248B (en) 2023-01-29 2023-01-29 High-precision manual embryo picking device

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Application Number Priority Date Filing Date Title
CN202310101259.4A CN116200248B (en) 2023-01-29 2023-01-29 High-precision manual embryo picking device

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CN116200248A CN116200248A (en) 2023-06-02
CN116200248B true CN116200248B (en) 2024-03-26

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0842778A (en) * 1994-08-04 1996-02-16 Aisan Ind Co Ltd Water hammer absorber
CN210237620U (en) * 2019-07-12 2020-04-03 贵州省人民医院 Egg picking needle for IVF-ET
CN112401987A (en) * 2020-11-05 2021-02-26 苏州法兰克曼医疗器械有限公司 Endoscope puncture needle capable of quantitatively controlling negative pressure
CN217611322U (en) * 2022-04-26 2022-10-21 李磊 Medical science is with embryo transfer ration pump drainage device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0842778A (en) * 1994-08-04 1996-02-16 Aisan Ind Co Ltd Water hammer absorber
CN210237620U (en) * 2019-07-12 2020-04-03 贵州省人民医院 Egg picking needle for IVF-ET
CN112401987A (en) * 2020-11-05 2021-02-26 苏州法兰克曼医疗器械有限公司 Endoscope puncture needle capable of quantitatively controlling negative pressure
CN217611322U (en) * 2022-04-26 2022-10-21 李磊 Medical science is with embryo transfer ration pump drainage device

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