CN116144333B - Super-thick oil biochemical viscosity reducer and preparation method thereof - Google Patents
Super-thick oil biochemical viscosity reducer and preparation method thereof Download PDFInfo
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- CN116144333B CN116144333B CN202111391222.7A CN202111391222A CN116144333B CN 116144333 B CN116144333 B CN 116144333B CN 202111391222 A CN202111391222 A CN 202111391222A CN 116144333 B CN116144333 B CN 116144333B
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- Prior art keywords
- biochemical
- viscosity reducer
- super
- solvent
- pseudomonas stutzeri
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/584—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific surfactants
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The invention relates to the technical field of thickened oil viscosity reducers, in particular to a biochemical viscosity reducer for super thickened oil and a preparation method thereof, wherein the raw materials comprise pseudomonas stutzeri mixed rhamnolipid solution, composite biological enzyme, plant-based zwitterionic surfactant, antibacterial agent, stabilizer and solvent, and the biochemical viscosity reducer is prepared by the following steps: at a certain temperature, mixing the pseudomonas stutzeri with the rhamnolipid solution, the compound biological enzyme, the plant-based zwitterionic surfactant and the stabilizer, stirring and mixing uniformly, adding the antibacterial agent and the solvent, stirring and mixing uniformly, cooling to room temperature, and standing to obtain the antibacterial agent. The biochemical viscosity reducer for the super-thick oil is prepared by combining a microbial metabolite rhamnose lipid biosurfactant and a compound biological enzyme, has the characteristics of excellent salt resistance and surface tension reduction, has remarkable viscosity reducing effect, can be completely degraded by microorganisms, is environment-friendly, safe and efficient.
Description
Technical Field
The invention relates to the technical field of thick oil viscosity reducers, in particular to a biochemical viscosity reducer for super thick oil and a preparation method thereof.
Background
The thick oil is used as an unconventional crude oil resource and accounts for more than 30% of the crude oil reserves which are ascertained in the world. The yield of light crude oil easy to be extracted is gradually reduced, and the demand of petroleum resources is gradually increased, so that the extraction of thick oil is more urgent. Thick oil, as its name implies, is a relatively viscous crude oil, mainly having a high content of colloidal asphaltenes, poor fluidity, and difficult exploitation and transportation. In addition, asphaltene precipitation can be inevitably caused in the heavy oil reservoir exploitation process to block an oil layer seepage pore canal, so that the yield is influenced, and even the production is stopped. The addition of the chemical viscosity reducer or the light crude oil is a conventional method for exploiting thick oil, but the former has the defects of difficult emulsification of thick oil stratum, difficult demulsification and dehydration in the later period, high cost, environmental protection and the like, and the latter consumes a large amount of light crude oil, has high cost and can not exploit oil reservoirs lacking light crude oil sources. Therefore, an environment-friendly thick oil viscosity reducer is required.
The microbial surfactant (BS) is a surfactant with an amphiphilic structure generated by microbial metabolism, and the BS can disperse and emulsify crude oil, so that the viscosity of the crude oil is greatly reduced, and the microbial surfactant is an important mechanism for microbial oil extraction at present. BS is mainly divided into glycolipids and lipopeptides, the former is produced by pseudomonas and the latter is produced by bacillus, and the BS can obviously reduce the oil-water interfacial tension, strip crude oil on the surface of rock pores and disperse and emulsify the crude oil.
Biological enzymes have been used to enhance crude oil recovery, see U.S. patent No. 9598944-Enzyme enhanced oil recovery, which are proteins with mild reaction conditions and specificity and are highly efficient catalysts. It is reported that its catalytic efficiency is 106 to 1014 times faster than that of a general chemical catalyst. Biological enzymes are microbiologically produced, environmentally friendly, surface active substances that generally function at very low concentrations, and injection of modified biological enzyme solutions in laboratory core displacement and field testing has been found to increase petroleum production. The main mechanism of biological enzyme oil displacement is that (1) protein films are formed on the surface of the rock, so that the wettability of the rock is changed to be more similar to a hydrophilic state, and the surfaces of the rock are made hydrophilic through hydrogen bonding with water molecules. (2) The viscosity or interfacial tension of the oil is reduced by emulsification, and since they are composed of hydrophilic and lipophilic molecules, they exhibit an amphiphilic structure, and can be micellised at the interface to reduce the interfacial tension. Among them, wettability is an important factor in controlling the location, flow and distribution of fluids in reservoirs, and several documents have discussed the effect of wettability on oil recovery, and many researchers have attempted to advantageously modify wettability in reservoirs to improve spontaneous absorption of water and water flooding properties, thereby enhancing recovery. Enzymes are a specific group of proteins synthesized by living cells and are catalysts for thousands of biochemical reactions. They can be used to produce both the desired chemicals and to degrade undesired chemicals. Mechanism of enzymatic chemical reaction: when the substrate enters the active site of the enzyme, the active site reduces the activation energy required for the reaction, accelerating or catalyzing the reaction. The substrate is then converted to a product, and finally the enzyme is released from the active site and recycled as a catalyst for the next reaction.
Enzymes include hydrolases, transferases, lyases, isomerases, ligases, oxidoreductases, and the like. Different enzymes have different roles, most enzymes currently used in the industry are hydrolases, including a variety of different types. For example: proteases (hydrolytic peptides), amidases (hydrolytic amides), haloenzymes (hydrolytic halides), esterases (hydrolytic esters) and lipases (hydrolytic triglycerides), which decompose certain compounds by hydrolysis. In recent years, many researchers have studied and reported the use of enzymes in the petroleum industry.
The fungi and enzymes produced by the fungi have decomposition effects on heavy components such as polycyclic aromatic hydrocarbon and colloid asphaltene in the thick oil, and become a new bright point in microbial oil recovery. Some microorganisms, such as fungi, are reported to oxidize asphaltenes because they have specific enzymes, such as peroxidases and laccases. Some heme proteins, such as chloroperoxidase, cytochrome c peroxidase, cytochrome c reductase, lignin peroxidase, and the like, from bacillus megaterium and escherichia coli lignin peroxidase, are capable of biocatalytically reforming asphaltenes to remove nickel and vanadium from petroleum porphyrins and asphaltenes. It has recently been reported that Daedaleopsssp isolated from wood degrading fungi has an effect of degrading asphaltenes and dibenzofurans. After 14 days of incubation at 40℃and pH 5, the microorganism consumed more asphaltenes and produced 88.7% asphaltene biodegradation in the crude oil. Clear experimental evidence of enzymes capable of modifying asphaltene molecules has been reported. The chloroperoxidase in the fungus aspergillus fumigatus can convert petroleum porphyrin and asphaltene, and the modification effect of the chloroperoxidase in the fungus aspergillus fumigatus is obviously improved in a system containing an organic solvent and a water system. Asphaltenes and petroleum porphyrins have been highly active in the cations of equine cardiac cytochrome C. The surface of the protein is chemically modified by polyethylene glycol to obtain the protein-polymer conjugate which is soluble in organic solvent. Furthermore, methyl esterification of heme propionate increases the hydrophobicity of the active site. The fungal enzyme degrades asphaltene in the thick oil, and removes heavy metals such as nickel, vanadium and the like, so that the viscosity of the thick oil can be effectively reduced, crude oil is stripped, and the fluidity of the thick oil is improved.
Chinese patent CN108130064a discloses a thick oil viscosity reducer of bacillus subtilis biosurfactant, but the anionic surfactant alkyl sulfonate and nonionic surfactant alkyl alcohol amine polyether are added in the patent, which cannot be completely degraded by microorganisms, increasing the later dehydration difficulty and environmental protection pressure.
Chinese patent CN108148565A discloses a viscosity reducer for preparing ionic liquid type thick oil by using microbial surfactant and its preparation method, and the patent introduces microbial lipopeptid into anionic or cationic liquid based on the above patent (CN 108130064 a), which increases cost and environmental protection risk.
At present, the detailed report of preparing the super heavy oil viscosity reducer by combining the microbial glycolipid surfactant and the fungal biological enzyme is not seen. The person skilled in the art needs to research and develop a non-toxic, harmless, pollution-free and corrosion-free super-heavy oil viscosity reducer product, which is safe and environment-friendly.
Disclosure of Invention
The invention provides a biochemical viscosity reducer for super heavy oil and a preparation method thereof, overcomes the defects of the prior art, has obvious viscosity reducing effect, can be completely degraded by microorganisms, and is environment-friendly, safe and efficient.
One of the technical schemes of the invention is realized by the following measures: the biochemical viscosity reducer for super thick oil consists of mixed rhamnolipid solution of pseudomonas stutzeri 30-70 wt%, composite biological enzyme 10-30 wt%, plant base zwitterionic surfactant 10-25 wt%, antiseptic 1-5 wt%, stabilizer 1-5 wt% and solvent for the rest.
The following are further optimizations and/or improvements to one of the above-described inventive solutions:
the complex biological enzyme is more than four of esterase, protease, oxidoreductase, laccase and peroxidase.
The plant-based zwitterionic surfactant is cocamidopropyl betaine.
The stabilizer is more than one of disodium EDTA and sodium citrate.
The antibacterial agent is one of THPS (THPS) and potassium benzoate.
The solvent is an alcohol solvent, and the alcohol solvent is at least one of ethylene glycol, propylene glycol and butanediol.
The biochemical viscosity reducer for the super heavy oil is prepared by the following steps: firstly, putting a needed amount of pseudomonas stutzeri mixed rhamnolipid solution, composite biological enzyme, plant-based zwitterionic surfactant and stabilizer into a reaction kettle, controlling the reaction temperature to be 30-60 ℃ and stirring for 50-90 min; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring for 60-120 min, cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
The second technical scheme of the invention is realized by the following measures: the preparation method of the biochemical viscosity reducer for the super heavy oil is carried out according to the following steps: firstly, placing a needed amount of pseudomonas stutzeri mixed rhamnolipid solution, composite biological enzyme, plant-based zwitterionic surfactant and stabilizer in a reaction kettle, controlling the reaction temperature to be 30-60 ℃ and stirring for 50-90 min; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring for 60-120 min, cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
The invention has the beneficial effects that:
in the super-heavy oil biochemical viscosity reducer, the pseudomonas stutzeri mixed rhamnolipid solution serving as a microbial metabolite rhamnolipid biosurfactant has the functions of wetting, emulsifying and dispersing, and is used for dissolving, solubilising and destroying heavy oil asphaltene aggregates;
the raw material composite biological enzyme adopts more than four of esterase, protease, oxidoreductase, laccase and peroxidase, can rapidly strip thick oil on the surface of rock minerals, destroy condensed ring aromatic structures formed by porphyrin heavy metals in the thick oil, and reduce the complexing force of the condensed ring aromatic hydrocarbons and the rock minerals;
the raw material plant-based amphoteric surfactant adopts cocamidopropyl betaine, can be combined with a biosurfactant, and can rapidly disperse and emulsify thick oil;
the raw material stabilizer adopts more than one of EDTA disodium and sodium citrate, so that the inactivation of high-valence ions in the oil reservoir environment on the microbial metabolite rhamnolipid biosurfactant can be effectively eliminated, and the organic precipitation is eliminated;
the raw material antibacterial agent adopts one of THPS (THPS) and potassium benzoate, can keep the long-term stability of the super heavy oil biochemical viscosity reducer, inhibit harmful microorganisms such as SRB (styrene-butadiene-styrene) and the like, can be completely degraded by microorganisms, and has no environmental hidden trouble;
the raw material solvent adopts an alcohol solvent, and the alcohol solvent is more than one of ethylene glycol, propylene glycol and butanediol, so that the synergistic effect of each active component in the super heavy oil biochemical viscosity reducer can be fully exerted.
According to the biochemical viscosity reducer for the super-thick oil, on one hand, a protein film is formed on the surface of the mineral of the thick oil storage rock, so that the wettability of the rock is changed, and the rock is more similar to a hydrophilic state. The protein membrane consists of hydrophilic amino (-NH 2) and carboxyl (-COOH) groups, which make the rock surface hydrophilic by hydrogen bonding with water molecules; on the other hand, the viscosity or interfacial tension of the oil is reduced through biological emulsification, and because the microbial metabolites of the rhamnolipid biosurfactant and the cocamidopropyl betaine are composed of hydrophilic and oleophilic molecules, the amphiphilic structure is shown, and the amphiphilic structure can be micellized at an oil-rock water interface, so that the interfacial tension is reduced.
In conclusion, the biochemical viscosity reducer for the super-thick oil is prepared by combining the microbial metabolite rhamnose lipid biosurfactant and the compound biological enzyme, has the characteristics of excellent salt resistance and surface tension reduction, has remarkable viscosity reducing effect, can be completely degraded by microorganisms, is environment-friendly, safe and efficient.
Detailed Description
The present invention is not limited by the following examples, and specific embodiments can be determined according to the technical scheme and practical situations of the present invention. The various chemical reagents and chemical supplies mentioned in the invention are all commonly known and used in the prior art unless specified otherwise; the percentages in the invention are mass percentages unless specified otherwise; the solutions in the invention are aqueous solutions in which the solvent is water unless otherwise specified, for example, the hydrochloric acid solution is hydrochloric acid aqueous solution; the room temperature and the room temperature in the present invention generally refer to temperatures ranging from 15 ℃ to 25 ℃, and are generally defined as 25 ℃.
The invention is further described below with reference to examples:
example 1: the biochemical viscosity reducer for the super heavy oil comprises, by weight, 30-70% of a pseudomonas stutzeri mixed rhamnolipid solution, 10-30% of a composite biological enzyme, 10-25% of a plant-based zwitterionic surfactant, 1-5% of an antibacterial agent, 1-5% of a stabilizer and the balance of a solvent.
The complex biological enzyme is more than four of esterase, protease, oxidoreductase, laccase and peroxidase.
The plant-based zwitterionic surfactant is cocamidopropyl betaine.
The stabilizer is more than one of disodium EDTA and sodium citrate.
The antibacterial agent is one of THPS (THPS) and potassium benzoate.
The solvent is an alcohol solvent, and the alcohol solvent is at least one of ethylene glycol, propylene glycol and butanediol.
The biochemical viscosity reducer for the super heavy oil is prepared by the following steps: firstly, putting a needed amount of pseudomonas stutzeri mixed rhamnolipid solution, composite biological enzyme, plant-based zwitterionic surfactant and stabilizer into a reaction kettle, controlling the reaction temperature to be 30-60 ℃ and stirring for 50-90 min; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring for 60-120 min, cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
The Pseudomonas stutzeri adopted in the mixed rhamnolipid solution of the Pseudomonas stutzeri serving as a raw material is provided by university of Yangtze river (address: university of Yangtze river, southern loop No. 1 of Jingzhou, hubei province).
Description of the preservation of Pseudomonas stutzeri:
strain name: pseudomonas stutzeri
Latin name: pseudomonas stutzeri
Preservation mechanism: china general microbiological culture Collection center (China Committee for culture Collection of microorganisms)
Preservation number: CGMCC No.7534
Preservation address: china academy of sciences of microorganisms, no. 3, north Chen West Lu 1, chaoyang, beijing, china
Preservation date: 28 th 2013, 04 th
Pseudomonas stutzeri mixed rhamnolipid solution:
the components and the amounts of the culture medium: 5.0g of NaCl, 1.8g of MgCl2, 0.02g of CaCl2, 0.3g of NH4Cl, 0.2g of K2HPO4, 0.5g of KCl, 3.0g of KNO3 (35 mM), 1.0g of sodium lactate and 1.0g of Yeast; trace elements 1ml, 0.1% resazurin 1ml, vitamin b complex solution 2ml and distilled water 1L, wherein the vitamin b complex solution comprises the following components in percentage by weight: 2.0g of biotin, 10.0g of pyridoxine hydrochloride, 5.0g of thiamine, 5.0g of D-calcium pantothenate, 5.0g of lipoic acid, 2.0g of folic acid, 5.0g of VB, 0.1g of VB12, 0.2g of VB6, 5.0g of para-aminobenzoic acid, 5.0g of nicotinic acid, 1.0g of vitamin C and 1L of distilled water.
Preparation of the culture medium: the components of the medium are mixed and then the pH value is adjusted to 7.0 to 7.2 by using HCl/NaOH (0.05 mol/L) solution.
Preparation of pseudomonas stutzeri mixed rhamnolipid solution: boiling the culture medium, introducing nitrogen to remove oxygen, subpackaging into 250ml anaerobic bottles, sterilizing at 121 deg.C under 0.1MPa for 20min to 30 min per bottle, cooling the culture medium, inoculating 5% Pseudomonas stutzeri strain with injection needle, and standing at 37deg.C for 15 days.
Example 2: the biochemical viscosity reducer for the super heavy oil comprises, by weight, 30% or 70% of a pseudomonas stutzeri mixed rhamnolipid solution, 10% or 30% of a composite biological enzyme, 10% or 25% of a plant-based zwitterionic surfactant, 1% or 5% of an antibacterial agent, 1% or 5% of a stabilizer and the balance of a solvent.
The complex biological enzyme is more than four of esterase, protease, oxidoreductase, laccase and peroxidase.
The plant-based zwitterionic surfactant is cocamidopropyl betaine.
The stabilizer is more than one of disodium EDTA and sodium citrate.
The antibacterial agent is one of THPS (THPS) and potassium benzoate.
The solvent is an alcohol solvent, and the alcohol solvent is at least one of ethylene glycol, propylene glycol and butanediol.
The biochemical viscosity reducer for the super heavy oil is prepared by the following steps: firstly, putting a needed amount of pseudomonas stutzeri mixed rhamnolipid solution, composite biological enzyme, plant-based zwitterionic surfactant and stabilizer into a reaction kettle, controlling the reaction temperature to be 30 ℃ or 60 ℃ and stirring for 50min or 90min; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring for 60min or 120min, and then cooling or standing at room temperature for 2 hours to obtain the biochemical viscosity reducer for the super-thick oil.
Example 3: the biochemical viscosity reducer for the super heavy oil comprises, by weight, 50% of a pseudomonas stutzeri mixed rhamnolipid solution, 18% of a compound biological enzyme (wherein, 5% of protease, 5% of esterase, 5% of oxido-reductase and 3% of laccase), 12% of cocamidopropyl betaine, 2% of an antibacterial agent (THPS), 3% of a stabilizer (EDTA disodium) and the balance of a solvent (propylene glycol and butylene glycol with the volume ratio of 1:1), wherein the viscosity reducer is prepared by the following steps: placing required amount of pseudomonas stutzeri mixed rhamnolipid solution, compound biological enzyme, cocamidopropyl betaine and stabilizer in a 2000 liter ceramic reactor, uniformly stirring, heating to 45 ℃, and preserving heat for 60 minutes; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuing stirring and preserving heat for 100 minutes, then slowly cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
Example 4: the biochemical viscosity reducer for the super heavy oil comprises 46% of pseudomonas stutzeri mixed rhamnolipid solution, 18% of compound biological enzyme (wherein, 4% of protease, 5% of esterase, 5% of oxido-reductase and 4% of laccase), 16% of cocamidopropyl betaine, 2.8% of antibacterial agent (THPS), 2% to 4% of stabilizer (sodium citrate 1% to 2% and EDTA disodium 1% to 2%) and the balance of solvent (ethylene glycol, propylene glycol and butanediol with the volume ratio of 1:1) according to the following method: placing required amount of pseudomonas stutzeri mixed rhamnolipid solution, compound biological enzyme, cocamidopropyl betaine and stabilizer into a 2000 liter ceramic reactor, uniformly stirring, heating to 55 ℃, and preserving heat for 95 minutes; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuing stirring and preserving heat for 88 minutes, then slowly cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
Example 5: the biochemical viscosity reducer for the super heavy oil comprises, by weight, 51% of a pseudomonas stutzeri mixed rhamnolipid solution, 14% of a compound biological enzyme (3.6% of protease, 2.4% of esterase, 4.5% of oxido-reductase and 2.5% of laccase), 10% of cocamidopropyl betaine, 4% of an antibacterial agent (2% of phosphorus tetramethylolsulfate THPS and 2% of potassium benzoate), 3% of a stabilizer (EDTA disodium) and the balance of a solvent (butanediol), wherein the viscosity reducer is prepared by the following steps: placing required amount of pseudomonas stutzeri mixed rhamnolipid solution, compound biological enzyme, cocamidopropyl betaine and stabilizer in a 2000 liter ceramic reactor, uniformly stirring, heating to 51 ℃, and preserving heat for 90 minutes; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring and preserving heat for 120 minutes, then slowly cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
Example 6: the biochemical viscosity reducer for the super heavy oil comprises, by weight, 42% of pseudomonas stutzeri mixed rhamnolipid solution, 22% of composite biological enzyme (wherein, 6% of protease, 5% of esterase, 5% of oxido-reductase and 5% of laccase), 17% of cocamidopropyl betaine, 3.8% of antibacterial agent (THPS), 5% of stabilizer (sodium citrate 2% and EDTA disodium 3%) and the balance of solvent (butanediol and propylene glycol with volume ratio of 1:1), and is prepared by the following method: placing required amount of pseudomonas stutzeri mixed rhamnolipid solution, compound biological enzyme, cocamidopropyl betaine and stabilizer into a 2000 liter ceramic reactor, uniformly stirring, heating to 58 ℃, and preserving heat for 90 minutes; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring and preserving heat for 95 minutes, then slowly cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
Example 7: the viscosity reduction effect of the super-thick oil biochemical viscosity reducer obtained in the embodiment 3 to the embodiment 6 is examined, detection items comprise viscosity (mPa.s) of dehydrated crude oil, viscosity reduction rate (%) of dehydrated crude oil, viscosity (mPa.s) of emulsified crude oil and viscosity reduction rate (%) of emulsified crude oil, and the detection items are carried out according to a method specified in general technical condition of the super-thick oil viscosity reducer of Q/SH1020 1519-2016, wherein the parameter condition of the viscosity meter is that the temperature is 60 ℃, the rotating speed is 200rpm, the torque is 70, test results are shown in the table 1, the super-thick oil biochemical viscosity reducer can enable original thick oil with the mineralization degree of 150000ppm (mass ratio) to be emulsified into oil-in-water emulsion, and the viscosity reduction rate of the emulsified crude oil reaches more than 99%, so that the super-thick oil biochemical viscosity reducer is salt-resistant and has good viscosity reduction effect.
In conclusion, the biochemical viscosity reducer for the super-thick oil is prepared by combining the microbial metabolite rhamnose lipid biosurfactant and the compound biological enzyme, has the characteristics of excellent salt resistance and surface tension reduction, has remarkable viscosity reducing effect, can be completely degraded by microorganisms, is environment-friendly, safe and efficient.
The technical characteristics form the embodiment of the invention, have stronger adaptability and implementation effect, and can increase or decrease unnecessary technical characteristics according to actual needs so as to meet the requirements of different situations.
Claims (5)
1. The biochemical viscosity reducer for the super-thick oil is characterized by comprising, by weight, 30-70% of a pseudomonas stutzeri mixed rhamnolipid solution, 10-30% of a compound biological enzyme, 10-25% of a plant-based zwitterionic surfactant, 1-5% of an antibacterial agent, 1-5% of a stabilizer and the balance of a solvent, wherein the plant-based zwitterionic surfactant is cocamidopropyl betaine, and the compound biological enzyme is more than four of esterase, protease, oxidoreductase, laccase and peroxidase; the preparation method of the pseudomonas stutzeri mixed rhamnolipid solution comprises the steps of boiling a culture medium, introducing nitrogen to remove oxygen, subpackaging the culture medium into 250ml anaerobic bottles, sterilizing each bottle for 20-30 min at the temperature of 0.1MPa and 121 ℃, after the culture medium is cooled, inoculating 5% of pseudomonas stutzeri strains into the culture medium by using an injection needle, and standing and culturing the culture medium at the temperature of 37 ℃ for 15 days respectively to obtain the pseudomonas stutzeri mixed rhamnolipid solution.
2. The biochemical viscosity reducer for the super heavy oil according to claim 1, wherein the stabilizer is more than one of disodium EDTA and sodium citrate; or/and the antibacterial agent is one of the phosphoric acid tetra-methylol sulfate and the potassium benzoate.
3. The biochemical viscosity reducer for ultra-thick oil according to claim 1 or 2, wherein the solvent is an alcohol solvent, and the alcohol solvent is one or more of ethylene glycol, propylene glycol and butanediol.
4. The biochemical viscosity reducer for ultra-thick oil according to claim 1 or 2, which is characterized by being obtained by the following method: firstly, putting a needed amount of pseudomonas stutzeri mixed rhamnolipid solution, composite biological enzyme, plant-based zwitterionic surfactant and stabilizer into a reaction kettle, controlling the reaction temperature to be 30-60 ℃ and stirring for 50-90 min; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring for 60-120 min, cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
5. A process for the preparation of a biochemical viscosity reducer for ultra-thick oils according to claim 1 or 2, characterized in that it is carried out according to the following method: firstly, putting a needed amount of pseudomonas stutzeri mixed rhamnolipid solution, composite biological enzyme, plant-based zwitterionic surfactant and stabilizer into a reaction kettle, controlling the reaction temperature to be 30-60 ℃ and stirring for 50-90 min; and secondly, adding the required amount of antibacterial agent and solvent into the reaction kettle, continuously stirring for 60-120 min, cooling to room temperature, and standing for 2 hours to obtain the super-thick oil biochemical viscosity reducer.
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| CN108298780A (en) * | 2018-01-19 | 2018-07-20 | 中国科学院沈阳应用生态研究所 | A kind of biological cleaner and application method of processing oily sludge |
| CN112159649A (en) * | 2020-08-07 | 2021-01-01 | 长江大学 | Biochemical viscosity reducer for thickened oil and preparation method thereof |
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| CN107556993A (en) * | 2016-07-01 | 2018-01-09 | 中国石油化工股份有限公司 | A kind of biological emulsifying pour depressor of resisting high temperature, high salt and preparation method thereof |
| CN108298780A (en) * | 2018-01-19 | 2018-07-20 | 中国科学院沈阳应用生态研究所 | A kind of biological cleaner and application method of processing oily sludge |
| CN112159649A (en) * | 2020-08-07 | 2021-01-01 | 长江大学 | Biochemical viscosity reducer for thickened oil and preparation method thereof |
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