CN116135510A - Processing technology of nucleic acid low-adsorption centrifuge tube - Google Patents

Abstract

The invention discloses a processing technology of a nucleic acid low-adsorption centrifuge tube, which aims at providing a separate processing technology, shaping in a mould and processing technology of the centrifuge tube with good nucleic acid low-adsorption effect.

Description

Processing technology of nucleic acid low-adsorption centrifuge tube

Technical Field

The invention relates to the technical field of medical equipment, in particular to a processing technology of a nucleic acid low-adsorption centrifuge tube.

Background

Centrifuge tubes are widely used today, especially in life sciences, for example: biochemistry, molecular biology, cell biology, microorganisms, and the like.

In education, teaching and scientific experiments, centrifuge tubes are widely used for sample preservation, chemical reaction, biological extraction and separation, strain preservation, cell and microorganism culture, genetic material interpretation and the like.

In hospitals, preservation and analytical testing of biological and chemical samples are also widely used in centrifuge tubes.

Conventionally, a centrifuge tube is subjected to surface treatment by a molded centrifuge tube, but the surface treatment degree of the centrifuge tube is different to easily influence the nucleic acid low adsorption effect of the centrifuge tube due to the limitation of the shape of the centrifuge tube.

Disclosure of Invention

Aiming at the defects existing in the prior art, the invention aims to provide a centrifugal tube processing technology which is used for separating processing, shaping in a mould and has good nucleic acid low adsorption effect.

In order to achieve the above purpose, the present invention provides the following technical solutions: a processing technology of a nucleic acid low-adsorption centrifuge tube comprises the following steps of S1, preparing a PP material, adding master batch with a low-adsorption function into the PP material in a molten state, melting at a high temperature and uniformly mixing;

s2, carrying out mirror surface treatment on the molding surface of the mold, setting the male mold structure as a multi-outer expansion piece structure, and arranging a driving cylinder for driving each outer expansion piece to reciprocate in the male mold structure;

s3, weaving the first glass fiber and the second glass fiber in a mixed mode to form a barrier layer structure, soaking the barrier layer structure in a low-adsorption treatment solution, and drying the barrier layer structure to obtain the composite barrier layer, wherein part of the fused PP material is mixed with the barrier layer structure to form a combined barrier layer;

s4, carrying out surface pretreatment on the combined barrier layer, sequentially removing oil and organic impurities from the centrifuge tube, and then carrying out hydroxylation treatment on the surface of the centrifuge tube;

s5, coating the surface of the combined barrier layer, dissolving a modified silane coupling agent in methanol, coating the surface of the hydroxylated combined barrier layer with a film, airing and performing heat treatment to obtain a coated combined barrier layer;

s6, preparing an acetone solution of 2.5-3wt% of benzophenone in the hydroxylation treatment specific operation in the surface pretreatment of the combined barrier layer, putting the acetone solution into the combined barrier layer, dipping the acetone solution for 20-25 min, taking out the acetone solution, and putting the acetone solution under an ultraviolet lamp for irradiation for 35-60S;

s7, sleeving the combined barrier layer on the male die structure through a manipulator, enabling one side of the net-shaped structure of the barrier layer to face towards the male die, and closing the upper die and the lower die;

s8, injecting the mixed molten PP material into a mold to wait for molding;

s9, gradually cooling the measuring die, pressing the barrier layer structure into the PP material by controlling a driving cylinder, and driving the distance of the external expansion sheet by the driving cylinder to be 0.1mm-0.3 mm;

s10, further cooling the die to obtain a finished centrifuge tube.

The invention is further provided with: the low adsorption treatment solution is prepared by mixing the following components in parts by weight: 60-65 parts of water; 35-40 parts of aqueous inorganic resin; 20-25 parts of polyethylene glycol; 10-12 parts of sodium dodecyl sulfate; 8-10 parts of nano graphite; 6-8 parts of nano titanium dioxide.

The invention is further provided with: the impregnation speed of the combined layer structure in the chemical low adsorption treatment solution is 0.5-1m/min; the drying temperature is 220-260 ℃ and the time is 30-50 minutes after the soaking; the silane coupling agent is trimethoxy silane coupling agent, methyl trimethoxy silane coupling agent or methyl triethoxy silane.

The invention is further provided with: the ratio of the outer diameter of the outer expansion sheet in the outer expansion state to the outer diameter of the outer expansion sheet in the original state is 1.1-1.3.

The invention is further provided with: the mesh density of the mesh structure of the barrier layer is between 1000 meshes and 1200 meshes, and the ratio between the thickness of the mesh structure layer and the thickness of the PP material layer of the barrier layer is between 0.8 and 1.

The invention is further provided with: the specific combination method between the combined barrier layer and the molten PP material in the die is as follows: s70, sleeving the combined barrier layer on the male die structure through a manipulator, and heating the male die;

s71, monitoring the melting state of the combined barrier layer in real time, and heating to 50-80 ℃ for forming the combined barrier layer;

s72, injecting the PP material in a molten state into a forming cavity of the die;

s73, pressurizing the molding cavity of the injected material, controlling the pressure in the molding cavity to be between 30 and 35kPa, controlling the time of the molding stage to be between 38 and 45 seconds,

s74, maintaining the pressure in the molding cavity, wherein the time of the pressure maintaining stage is controlled to be 320-400 seconds, and the pressure in the molding cavity is controlled to be 30-35 kPa;

s75, controlling the time of the pressure relief stage within 10-20S, and controlling the time of the cooling stage within 250-300S;

s76, completing the combination step.

By adopting the technical scheme, the method has the beneficial effects that 1, by adopting the prepared PP material and adding the master batch with the low adsorption function into the PP material in a molten state, the master batch with the low adsorption function is used as a forming element with the low adsorption function, after being melted at a high temperature and uniformly mixed, the uniformity is higher, and further, the forming surface of the die is subjected to mirror surface treatment, so that the surface flatness of the centrifuge tube during forming in the die is improved, and the low adsorption effect is improved;

2. the composite barrier layer is formed by mixing and weaving the first glass fiber and the second glass fiber, soaking the composite barrier layer in the low-adsorption treatment solution and drying the composite barrier layer, and mixing part of the molten PP material with the composite barrier layer to form a combined barrier layer;

3. the barrier layer subjected to the low adsorption surface treatment and the PP material are formed in the die again, so that the low adsorption capacity of the centrifuge tube after forming is improved, the practicability is high, the split type treatment is integrated through the die, the overall low adsorption effect of the centrifuge tube is realized, and the coverage of the low adsorption area is wide;

4. in the processing technology of the invention, in order to ensure that the combined barrier layer and the PP material in a molten state can be well fused in the die, a good bonding effect is realized by heating the male die, and the combined barrier layer can be fully bonded in the die by pressurizing and then maintaining pressure after pressurizing, so that the stability is strong.

Drawings

FIG. 1 is a flow chart of an embodiment of a process for processing a nucleic acid low adsorption centrifuge tube according to the present invention.

Detailed Description

An example of a process for processing a nucleic acid low adsorption centrifuge tube according to the present invention is further described with reference to FIG. 1.

Spatially relative terms, such as "upper," "lower," "left," "right," and the like, may be used in the embodiments for ease of description to describe one element or feature's relationship to another element or feature's illustrated in the figures. It will be understood that the spatial terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "under" other elements or features would then be oriented "over" the other elements or features. Thus, the exemplary term "lower" may encompass both an upper and lower orientation. The device may be otherwise positioned (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

Moreover, relational terms such as "first" and "second", and the like, may be used solely to distinguish one element from another element having the same name, without necessarily requiring or implying any actual such relationship or order between such elements.

A processing technology of a nucleic acid low-adsorption centrifuge tube comprises the following steps of S1, preparing a PP material, adding master batch with a low-adsorption function into the PP material in a molten state, melting at a high temperature and uniformly mixing;

s2, carrying out mirror surface treatment on the molding surface of the mold, setting the male mold structure as a multi-outer expansion piece structure, and arranging a driving cylinder for driving each outer expansion piece to reciprocate in the male mold structure;

by adopting the prepared PP material and adding the master batch with the low adsorption function into the PP material in a molten state, taking the master batch with the low adsorption function as a forming element with the low adsorption function, after being melted at a high temperature and uniformly mixed, the uniformity is higher, further carrying out mirror surface treatment on the forming surface of the die, increasing the surface flatness of the centrifuge tube during forming in the die, thereby improving the low adsorption effect,

s3, weaving the first glass fiber and the second glass fiber in a mixed mode to form a barrier layer structure, soaking the barrier layer structure in a low-adsorption treatment solution, and drying the barrier layer structure to obtain the composite barrier layer, wherein part of the fused PP material is mixed with the barrier layer structure to form a combined barrier layer;

the composite barrier layer is formed by mixing and weaving the first glass fiber and the second glass fiber, soaking the composite barrier layer in the low-adsorption treatment solution and drying the composite barrier layer, and mixing part of the molten PP material with the composite barrier layer to form a combined barrier layer;

s4, carrying out surface pretreatment on the combined barrier layer, sequentially removing oil and organic impurities from the centrifuge tube, and then carrying out hydroxylation treatment on the surface of the centrifuge tube;

s5, coating the surface of the combined barrier layer, dissolving a modified silane coupling agent in methanol, coating the surface of the hydroxylated combined barrier layer with a film, airing and performing heat treatment to obtain a coated combined barrier layer;

s6, preparing an acetone solution of 2.5-3wt% of benzophenone in the hydroxylation treatment specific operation in the surface pretreatment of the combined barrier layer, putting the acetone solution into the combined barrier layer, dipping the acetone solution for 20-25 min, taking out the acetone solution, and putting the acetone solution under an ultraviolet lamp for irradiation for 35-60S;

s7, sleeving the combined barrier layer on the male die structure through a manipulator, enabling one side of the net-shaped structure of the barrier layer to face towards the male die, and closing the upper die and the lower die;

s8, injecting the mixed molten PP material into a mold for molding, and molding the barrier layer subjected to the low-adsorption surface treatment and the PP material in the mold again, so that the low adsorption capacity of the centrifuge tube after molding is improved, the practicability is high, and the split type treatment is integrated through the mold, so that the overall low adsorption effect of the centrifuge tube is realized, and the coverage of the low adsorption area is wide;

s9, gradually cooling the measuring die, pressing the barrier layer structure into the PP material by controlling the driving cylinder, wherein the distance between the driving cylinder and the outer expansion sheet is 0.1mm-0.3mm, and the steps are adopted for operation, so that the barrier layer structure and the PP material are fully combined in the die, a good combining effect is improved, and the practicability is high;

s10, further cooling the die to obtain a finished centrifuge tube.

The invention further provides that the low adsorption treatment solution is prepared by mixing the following components in parts by weight: 60-65 parts of water; 35-40 parts of aqueous inorganic resin; 20-25 parts of polyethylene glycol; 10-12 parts of sodium dodecyl sulfate; 8-10 parts of nano graphite; 6-8 parts of nano titanium dioxide.

The invention is further arranged that the dipping speed of the combined layer structure in the chemical low adsorption treatment solution is 0.5-1m/min; the drying temperature is 220-260 ℃ and the time is 30-50 minutes after the soaking; the silane coupling agent is trimethoxy silane coupling agent, methyl trimethoxy silane coupling agent or methyl triethoxy silane.

By adopting the structure, the net structure of the barrier layer is subjected to low adsorption treatment, the overall low adsorption effect is realized, the mesh density of the net structure of the barrier layer is between 1000 meshes and 1200 meshes, the integrity between the net structure and the PP material is ensured by controlling the mesh density of the net structure of the barrier layer, the overall low adsorption effect is improved, the ratio between the thickness of the layer of the net structure and the thickness of the PP material of the barrier layer is between 0.8 and 1, the formation of good integrity in a die is ensured, and the practicability is strong.

The invention is further arranged that the ratio between the outer diameter of the outer expansion sheet in the outer expansion state and the outer diameter of the outer expansion sheet in the original state is 1.1-1.3.

The invention further provides a specific combination method between the combined barrier layer and the molten PP material in the die, which comprises the following steps: s70, sleeving the combined barrier layer on the male die structure through a manipulator, and heating the male die;

s71, monitoring the melting state of the combined barrier layer in real time, and heating to 50-80 ℃ for forming the combined barrier layer;

s72, injecting the PP material in a molten state into a forming cavity of the die;

s73, pressurizing the molding cavity of the injected material, controlling the pressure in the molding cavity to be between 30 and 35kPa, controlling the time of the molding stage to be between 38 and 45 seconds,

s74, maintaining the pressure in the molding cavity, wherein the time of the pressure maintaining stage is controlled to be 320-400 seconds, and the pressure in the molding cavity is controlled to be 30-35 kPa;

s75, controlling the time of the pressure relief stage within 10-20S, and controlling the time of the cooling stage within 250-300S;

s76, completing the combination step.

In the processing technology of the invention, in order to ensure that the combined barrier layer and the PP material in a molten state can be well fused in the die, a good bonding effect is realized by heating the male die, and the combined barrier layer can be fully bonded in the die by pressurizing and then maintaining pressure after pressurizing, so that the stability is strong.

The preparation method of the aqueous inorganic resin comprises the following steps of S1, preparing raw materials of 60 parts by weight of potassium silicate, 20 parts by weight of silica sol; lithium silicate, 5 parts; 4 parts of a silane coupling agent; 10 parts of styrene-acrylic emulsion; 2 parts of magnesium chloride, and disodium ethylenediamine tetraacetate which is 4 times of the weight of the magnesium chloride; deionized water, 20 parts; the modulus of the potassium silicate is 3.3-3.5, and the silicon dioxide content is 19-21 wt%; the silica content in the silica sol is 25-26wt% and the sodium oxide content is 0.2-0.4 wt%; the modulus of lithium silicate is 4.7-4.9, and the content of silicon dioxide is 19-21 wt%; the solid content of the styrene-acrylic emulsion is 46-48wt%;

s2, adding deionized water into a dispersion kettle, regulating the rotating speed to 700r/min, adding potassium silicate, silica sol and lithium silicate, and continuously stirring for 5 minutes;

and S3, finally, dropwise adding the silane coupling agent within 20 minutes, regulating the rotating speed to 500r/min, continuously stirring for 1.5 hours, standing for 30 hours, adding the rest raw materials, continuously stirring for 50 minutes, and filtering to obtain the finished product.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention, but one skilled in the art can make common changes and substitutions within the scope of the technical solution of the present invention.

Claims (6)

1. A processing technology of a nucleic acid low adsorption centrifuge tube is characterized by comprising the following steps of S1, preparing materials: preparing a PP material, adding master batch with a low adsorption function into the PP material in a molten state, melting at a high temperature, and uniformly mixing;

s2, mirror surface treatment of a die: mirror surface treatment is carried out on the molding surface of the mold, the male mold structure is set to be a multi-outer expansion piece structure, and a driving cylinder for driving each outer expansion piece to reciprocate is arranged in the male mold structure;

s3, forming a barrier layer structure: mixing and weaving the first glass fiber and the second glass fiber to form a barrier layer structure, soaking the barrier layer structure in a low-adsorption treatment solution, and drying the barrier layer structure to obtain the composite barrier layer, wherein part of the fused PP material is mixed with the barrier layer structure to form the composite barrier layer;

s4, primary treatment of the surface of the combined barrier layer: the surface pretreatment is carried out on the combined barrier layer, firstly, the oil removal and the organic impurity cleaning are sequentially carried out on the centrifuge tube, and then the hydroxylation treatment is carried out on the surface of the centrifuge tube;

s5, surface coating treatment of the combined barrier layer: dissolving a modified silane coupling agent in methanol, coating a film on the surface of the combined barrier layer subjected to hydroxylation treatment, airing, and performing heat treatment to obtain a coated combined barrier layer;

s6, carrying out hydroxylation treatment on the surface of the combined barrier layer: specifically, preparing 2.5-3 wt% benzophenone acetone solution, putting into a combined barrier layer, soaking for 20-25 min, taking out, and putting under an ultraviolet lamp for irradiation for 35-60 s;

s7, feeding a combined barrier layer: sleeving the combined barrier layer on the male die structure through a mechanical arm, enabling one side of the net-shaped structure of the barrier layer to face the male die, and closing the upper die and the lower die;

s8, injection molding: injecting the mixed molten PP material into a mold to wait for molding;

s9, cooling and extruding: gradually cooling the measuring die, pressing the barrier layer structure into the PP material by controlling a driving cylinder, and driving the distance between the driving cylinder and the outer expansion sheet to be 0.1mm-0.3 mm;

s10, cooling is completed: and (5) further cooling the die to obtain the finished centrifuge tube.

2. The process for manufacturing a low adsorption centrifuge tube for nucleic acid according to claim 1, wherein the low adsorption treatment solution is prepared by mixing the following components in parts by weight: 60-65 parts of water; 35-40 parts of aqueous inorganic resin; 20-25 parts of polyethylene glycol; 10-12 parts of sodium dodecyl sulfate; 8-10 parts of nano graphite; 6-8 parts of nano titanium dioxide.

3. The process for manufacturing a nucleic acid low adsorption centrifuge tube according to claim 2, wherein the immersing speed of the combined layer structure in the chemical low adsorption treatment solution is 0.5-1m/min; the drying temperature is 220-260 ℃ and the time is 30-50 minutes after the soaking; the silane coupling agent is trimethoxy silane coupling agent, methyl trimethoxy silane coupling agent or methyl triethoxy silane.

4. The process for manufacturing a low adsorption centrifuge tube for nucleic acid according to claim 1, wherein the ratio between the outer diameter of the swelling sheet in the swollen state and the outer diameter of the swelling sheet in the original state is 1.1 to 1.3.

5. The process of claim 1, wherein the barrier layer has a mesh density of between 1000 mesh and 1200 mesh and the ratio between the thickness of the mesh layer and the thickness of the PP material layer of the barrier layer is between 0.8 and 1.

6. The process for manufacturing a low adsorption centrifuge tube for nucleic acids according to claim 1, wherein the specific combination method between the combined barrier layer and the molten PP material in the mold is as follows: s70, sleeving the combined barrier layer on the male die structure through a manipulator, and heating the male die;

s71, monitoring the melting state of the combined barrier layer in real time, and heating to 50-80 ℃ for forming the combined barrier layer;

s72, injecting the PP material in a molten state into a forming cavity of the die;

s73, pressurizing the inside of the molding cavity of the injected material, controlling the pressure in the molding cavity to be between 30 and 35kPa, and controlling the time of the molding stage to be between 38 and 45 seconds;

s74, maintaining the pressure in the molding cavity, wherein the time of the pressure maintaining stage is controlled to be 320-400 seconds, and the pressure in the molding cavity is controlled to be 30-35 kPa;

s75, controlling the time of the pressure relief stage within 10-20S, and controlling the time of the cooling stage within 250-300S;

s76, completing the combination step.

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