CN110725023A - Preparation method of ultrathin cavity composite microfiber material based on microfluidic technology - Google Patents
Preparation method of ultrathin cavity composite microfiber material based on microfluidic technology Download PDFInfo
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/18—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from other substances
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/08—Addition of substances to the spinning solution or to the melt for forming hollow filaments
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
Abstract
The invention provides a preparation method of an ultrathin cavity composite microfiber material based on a microfluidic technology, which is characterized in that in the process of preparing common cavity microfibers, a modification material capable of being combined with an outer-layer microfiber material is introduced into an inner cavity of the microfiber, the modification material is attached to the cavity to form a modification coating while the cavity is formed, and then the outer-layer material is dissolved out to obtain the ultrathin microfiber material. The invention utilizes the micro-fluidic chip technology to form a micron-sized channel capable of generating a coaxial laminar flow pattern, realizes the flow pattern control of the sample fluid, finally solidifies the sample fluid into a micron-sized cavity fiber material with a specific inner coating structure, and obtains the ultrathin cavity composite microfiber material by removing an outer layer material. The microfiber material can simulate the microstructure in human tissues, and provides a new method and thought for tissue engineering and organ regeneration.
Description
Technical Field
The invention relates to a preparation method of an ultrathin cavity composite microfiber material, and particularly provides a preparation method of an ultrathin cavity composite microfiber material based on a microfluidic technology.
Background
The interfacial complexation method is to self-assemble two polyelectrolytes with opposite charges into a macromolecular complex by electrostatic force. Microcapsules, films, multilayer films, and the like can be prepared by this method. Yamamoto and his colleagues have for the first time proposed a method of forming fibers by the combination of two oppositely charged polyelectrolytes at interfacial contact via electrostatic forces. In the process, they carefully drop gellan gum aqueous solution into chitosan aqueous solution, form a complex film at the interface of the chitosan aqueous solution and the gellan gum aqueous solution without stirring at 50 ℃, and form fiber after drying. Wan further develops this process to produce finer fibers. First, they place two polyelectrolyte solutions of 5-20. mu.l each with opposite charges in close proximity, and then gently draw the two droplets into contact, fibers are formed at the interface between the two solutions with opposite charges, and the fibers can be collected by vertically drawing them with tweezers at the interface between the two solutions. The basic process of preparing fiber by interfacial complexation is that firstly polyelectrolyte complex fiber is formed at the interface of two solutions with opposite charges, micron-sized fiber cores are formed when the polyelectrolyte complex fiber is stretched, and the fiber is formed along with the growth and the aggregation of the fiber cores.
The process for preparing the fiber by the interface complexing method based on the polyelectrolyte with opposite charges is mild, and the fiber can be prepared in aqueous solution at room temperature, but the large-scale preparation is difficult to realize. Mainly because the flow rate must be controlled within the advection in order to form a stable contact interface of two polyelectrolytes with different charges, which limits the mixing and subsequent preparation of large flows. In addition, the fiber diameter is approximately close to one cell, so making smooth fiber entrapping cells is challenging. This limits the current small-scale laboratory studies of this method.
While microfluidic technology is directed to very small quantities (10)-3～10-12Microliter) of fluid, thus making possible the implementation and extension of the above-described techniques for preparing fibers by interfacial complexation. The microfluidic chip is a main platform and a technical device realized by the microfluidic technology, and is mainly characterized in that an effective structure for containing fluid is at least one dimensionAnd (4) micron-scale dimension. In this scale, the movement of the fluid has its own characteristics. Due to the characteristics of rapid mass and heat transfer and easy control of the microfluidic spinning technology, the structure, morphology and composition of the fiber can be accurately controlled, and a good micro-reaction platform is provided for preparing the functional heterogeneous hybrid microfiber.
A corresponding microfluidic chip is designed by utilizing a microfluidic technology, the cavity composite microfiber is prepared by interface complexation on a limited contact interface of two fluids by utilizing the electrostatic interaction of polyelectrolytes with opposite charges, and then the microfiber material on the outer layer is dissolved, so that the ultrathin cavity composite microfiber material is obtained. By this method, a large-scale production of microfibers can be achieved.
Disclosure of Invention
The invention aims to provide a preparation method of an ultrathin cavity composite microfiber material based on a microfluidic technology, which has an excellent technical effect. The invention provides a method for preparing novel ultrathin cavity composite microfiber by using a microfluidic chip platform which is simple to operate and high in flux.
The invention provides a preparation method of an ultrathin cavity composite microfiber material based on a microfluidic technology, which is characterized by comprising the following steps of: during the preparation of common cavity microfiber, the inner cavity of microfiber is introduced with decorating material capable of being combined with microfiber material, the decorating material is adhered to the cavity to form one decorating coating while the cavity is formed, and the outer layer material is dissolved to obtain the ultrathin microfiber material comprising inner coating material and outer thin layer material.
The microfluidic chip is formed by sealing an upper layer chip and a lower layer chip, wherein the two layers are made of polydimethylsiloxane materials; the chip has at least three parallel channel inlets, a common outlet, and a plurality of coaxial laminar flow channels connected to the inlets and outlets.
The micro-fluidic chip is of a four-channel structure and consists of four parallel channel inlets, four independent coaxial laminar flow channels and a main outlet,
the four parallel channel inlets are a sheath fluid inlet, a sample fluid inlet, an inner cavity modification fluid inlet and a central inert fluid inlet,
the four independent coaxial laminar flow channels are a sheath flow fluid channel, a sample fluid channel, an inner cavity modification fluid channel and a central inert fluid channel from inside to outside in sequence,
the general outlet is a microfiber material outlet.
A method for preparing an ultrathin cavity composite microfiber material based on a microfluidic technology comprises the following specific steps:
(1) preparing a sample fluid with a mass volume concentration of 1-5% g/ml; an inert fluid having a mass volume concentration of 1-3% g/ml; modifying the fluid at a mass volume concentration of 0.25-5%; a sheath flow fluid having a mass volume concentration of 0.5-5% g/ml;
(2) adopting a micro-fluidic chip, controlling the flow rate of sheath flow fluid by using an air pressure pump, controlling the flow rates of fluids of other three passages by using a Harvard pump, and introducing inert fluid, modifying fluid, sample fluid and sheath flow fluid into the chip in sequence; the flow rates of the first three are respectively 0.1-5 mul/min, 0.1-5 mul/min and 1-40 mul/min; the pressure of the pneumatic pump of the sheath flow fluid is 20-200 mbar; preparing an ultrathin cavity composite microfiber material;
(3) and soaking the prepared ultrathin hollow cavity composite microfiber material in a solution capable of dissolving the outer layer material for 10-100min to obtain the ultrathin microfiber material.
The sample fluid is a biological material that can be rapidly solidified;
the modified fluid is molecules and derivatives thereof with opposite charges with the sample fluid, or molecules and derivatives thereof capable of having certain interaction with the sample fluid, or molecules and derivatives thereof with strong adhesion;
the central inert fluid refers to inert water-soluble materials and derivatives thereof which do not react with the sample fluid;
the sheath flow fluid refers to a cross-linker solution of the sample fluid.
The sample fluid is one or a combination of the following: sodium alginate, polyethylene glycol diacrylate, chitosan and polylysine;
the modifying fluid is one or a combination of the following fluids: chitosan, chitin, polylysine, polydopamine, hyaluronic acid, agarose, collagen, laminin, fibronectin, type III collagen or serum expansion factor;
the central inert fluid is one or a combination of the following fluids: methyl cellulose, hydroxymethyl cellulose, polyvinyl alcohol or polyethylene oxide;
the sheath flow fluid is one or a combination of the following fluids: CaCl capable of rapidly crosslinking sodium alginate2Solutions or Ca2+、Cu2+、Ba2+A solution of a multivalent ion; or a sodium tripolyphosphate solution capable of cross-linking chitosan; for polyethylene glycol diacrylate that can be cured without solution curing such as ultraviolet light, the sheath fluid may be an inert buffer solution.
The solution for dissolving the outer layer material is phosphate solution capable of combining calcium ions, lysozyme solution capable of performing enzymolysis on the outer layer material, or other solutions capable of dissolving the outer layer material.
Before the preparation of the ultrathin cavity composite microfiber, the chip is introduced into a perfluorinated solution for surface hydrophobic treatment.
The ultrathin cavity composite microfiber material based on the microfluidic technology is prepared by the preparation method of the ultrathin cavity composite microfiber material, wherein the inner side of a cavity is modified;
during the process of preparing the microfibers, the density and thickness of the internal coating of the microfibers can be precisely controlled by controlling the concentration and flow rate of the modifying fluid.
A method for preparing an ultrathin cavity composite microfiber material based on a microfluidic technology comprises the following specific steps:
(1) sodium alginate with mass volume concentration of 1-5% g/ml is used as a sample fluid in advance; methyl cellulose with mass volume concentration of 1-3% g/ml is used as inert fluid; preparing a chitosan solution with the mass volume concentration of 0.5-10% g/ml by using acetic acid with the volume percentage of 0.25-5% as a modifying fluid; CaCl with mass volume concentration of 0.5-5% g/ml2As a sheath flow fluid;
(2) CaCl is controlled by adopting a microfluidic chip and an air pressure pump2The flow rate of the fluids in other three passages is controlled by a Harvard pump, and an inert fluid, namely methyl cellulose, a modified fluid, namely chitosan or a mixed solution of chitosan and methyl cellulose, sample fluid sodium alginate and sheath flow fluid CaCl are introduced into the chip in sequence2(ii) a The flow rates of the first three are respectively 0.1-5 mul/min, 0.1-5 mul/min and 1-40 mul/min; the pressure of the pneumatic pump of the sheath flow fluid is 20-200 mbar; preparing the ultrathin composite microfiber material with the cavity,
(3) and soaking the prepared ultrathin hollow-cavity composite microfiber material in a PBS (pH7.4) solution for 10-100min to obtain the ultrathin microfiber material.
The invention provides a method for preparing an ultrathin cavity composite microfiber material based on a microfluidic chip technology, which is characterized in that a plurality of coaxial laminar flow channels are arranged, and the coaxial laminar flow channels correspond to independent fluid inlets. The microfiber with a modified cavity can be obtained by introducing an inert fluid, a modifying fluid, a sample fluid and a sheath fluid into the channel from inside to outside in sequence. After the sample material of the outer layer is dissolved, a thin layer of the sample material is remained in the microfiber with the modified coating, and the ultrathin cavity composite microfiber material consisting of the sample material and the inner coating material is obtained. The preparation method is simple, the prepared material has a novel structure, and the preparation method is believed to have a great potential application value in tissue engineering construction.
The invention forms a micron-sized channel capable of generating a coaxial laminar flow pattern by utilizing a micro-fluidic chip technology and designing a micro-fluidic channel, realizes the flow pattern control of the sample fluid, and finally solidifies the sample fluid into a micron-sized fiber material with a specific structure. By selecting proper sample fluid, modification fluid and inert fluid, a new cavity composite fiber system suitable for long-term cell culture and functional tissue formation is prepared. Wherein the introduction of the modifying solution can endow the novel microfiber material with certain biological properties. The method simulates the microstructure in human tissues by preparing the functional new fiber material, and provides a new method and thought for tissue engineering and organ regeneration. The novel functional microfiber material is believed to have wide application prospects in the fields of tissue engineering and regenerative medicine.
The invention has the following advantages:
(1) the two-step method for preparing the ultrathin cavity composite microfiber material has the advantages of simple and reliable operation method and high efficiency, provides convenient conditions for the modification of microfibers, and is favorable for the mass preparation of microfibers.
(2) The internal decorative coating can also enhance the mechanical property of the microfiber, and provides mechanical property support for the construction of later-stage tissue engineering.
Drawings
The invention is described in further detail below with reference to the following figures and embodiments:
FIG. 1 is a schematic diagram of a microfluidic chip structure;
FIG. 2 is a diagram of a conventional hollow-cavity composite microfiber material;
FIG. 3 is a diagram of an ultra-thin cavity composite microfiber material;
fig. 4 is a partially enlarged view of fig. 3.
Wherein: 1 is a sheath fluid inlet, 2 is a sample fluid inlet, 3 is a lumen modifying fluid inlet, 4 is a central inert fluid inlet, 5 is a sheath fluid channel, 6 is a sample fluid channel, 7 is a lumen modifying fluid channel, 8 is a central inert fluid channel, and 9 is a microfiber material outlet.
Detailed Description
A preparation method of an ultrathin cavity composite microfiber material based on a microfluidic chip technology is characterized in that in the process of preparing cavity microfibers, a modification material capable of being combined with a microfiber material is introduced into an inner cavity of the microfibers, the modification material is attached to the cavity to form a modification coating while the cavity is formed, and an outer layer material is dissolved at a later stage to obtain the ultrathin cavity composite microfiber material.
The microfluidic chip is formed by sealing an upper layer chip and a lower layer chip, wherein the two layers are made of polydimethylsiloxane materials; the chip has at least three parallel channel inlets, a common outlet, and a plurality of coaxial laminar flow channels connected to the inlets and outlets.
As shown in fig. 1, the microfluidic chip in the embodiment of the present invention has a four-channel structure, and includes four parallel channel inlets, four independent coaxial laminar flow channels, and a total outlet.
The four parallel channel inlets are a sheath fluid inlet 1, a sample fluid inlet 2, a lumen modifying fluid inlet 3 and a central inert fluid inlet 4.
The four independent coaxial laminar flow channels are a sheath flow fluid channel 5, a sample fluid channel 6, an inner cavity modification fluid channel 7 and a central inert fluid channel 8 from inside to outside in sequence.
The general outlet is a microfiber material outlet 9.
Example 1
Sodium alginate with mass volume concentration of 1% g/ml is used as a sample fluid in advance; methylcellulose with a mass volume concentration of 2% g/ml is used as an inert fluid; preparing a chitosan solution with the mass volume concentration of 3% g/ml as a modified fluid by using acetic acid with the volume percentage of 1.5%, or mixing chitosan and methylcellulose to obtain methylcellulose solutions with different chitosan concentrations as the modified fluid; mass volume concentration of 0.5% g/ml CaCl2As a sheath flow fluid;
using a microfluidic chip, using the microfluidic chip shown in FIG. 1, and using a pneumatic pump to control CaCl2The flow rate of the fluids in other three passages is controlled by a Harvard pump, and an inert fluid, namely methyl cellulose, a modified fluid, namely chitosan or a mixed solution of chitosan and methyl cellulose, sample fluid sodium alginate and sheath flow fluid CaCl are introduced into the chip in sequence2(ii) a The flow rates of the first three are respectively 0.4 mul/min, 0.1 mul/min and 5 mul/min; the pressure of the pneumatic pump of the sheath flow fluid is 40 mbar;
soaking the prepared sodium alginate/chitosan composite hollow-cavity microfiber (figure 2) in a PBS (pH7.4) solution to obtain the ultrathin hollow-cavity composite microfiber material (figures 3 and 4).
Example 2
Sodium alginate with mass volume concentration of 2% g/ml is used asA sample fluid; methyl cellulose with mass volume concentration of 3% g/ml is used as inert fluid; preparing a chitosan solution with the mass volume concentration of 4% g/ml by using acetic acid with the volume percentage of 2% as a modified fluid, or mixing chitosan and methylcellulose to obtain methylcellulose solutions with different chitosan concentrations as the modified fluid; mass volume concentration of 1% g/ml CaCl2As a sheath flow fluid;
CaCl is controlled by adopting a microfluidic chip and an air pressure pump2The flow rate of the other three channels is controlled by a Harvard pump, an inert fluid containing cells, namely methyl cellulose, a modified fluid, namely chitosan or mixed solution of chitosan and methyl cellulose, a sample fluid sodium alginate and a sheath fluid CaCl are introduced into the chip in sequence2(ii) a The flow rates of the first three are respectively 0.8 mul/min, 0.2 mul/min and 10 mul/min; the pressure of the pneumatic pump of the sheath flow fluid is 80 mbar;
soaking the prepared sodium alginate/chitosan composite hollow-cavity microfiber (figure 2) in a PBS (pH7.4) solution to obtain the ultrathin hollow-cavity composite microfiber material (figures 3 and 4).
Example 3
Sodium alginate with mass volume concentration of 5% g/ml is used as a sample fluid in advance; methylcellulose with a mass volume concentration of 2% g/ml is used as an inert fluid; preparing a chitosan solution with the mass volume concentration of 5% g/ml by using acetic acid with the volume percentage of 2.5% as a modified fluid, or mixing chitosan and methylcellulose to obtain methylcellulose solutions with different chitosan concentrations as the modified fluid; CaCl with mass volume concentration of 2% g/ml2As a sheath flow fluid;
CaCl is controlled by adopting a microfluidic chip and an air pressure pump2The flow rate of the other three channels is controlled by a Harvard pump, an inert fluid containing cells, namely methyl cellulose, a modified fluid, namely chitosan or mixed solution of chitosan and methyl cellulose, a sample fluid sodium alginate and a sheath fluid CaCl are introduced into the chip in sequence2(ii) a The flow rates of the first three are respectively 1.5 mul/min, 0.3 mul/min and 20 mul/min; the pressure of the pneumatic pump of the sheath flow fluid is 100 mbar;
soaking the prepared sodium alginate/chitosan composite hollow-cavity microfiber (figure 2) in a PBS (pH7.4) solution to obtain the ultrathin hollow-cavity composite microfiber material (figures 3 and 4).
Claims (10)
1. A preparation method of an ultrathin cavity composite microfiber material based on a microfluidic technology is characterized by comprising the following steps: by adopting the micro-fluidic chip, in the process of preparing the microfiber with a common cavity, a modifying material capable of being combined with the microfiber material of the outer layer is introduced into the inner cavity of the microfiber, the modifying material is adhered to the cavity to form a modifying coating while the cavity is formed, and the microfiber material of the outer layer is dissolved away, so that a thin layer combined with the inner modifying material is stably remained, and the ultrathin composite microfiber material with the cavity is obtained.
2. The method for preparing the ultra-thin cavity composite microfiber material based on the microfluidic technology according to claim 1, wherein the method comprises the following steps: the microfluidic chip is formed by sealing an upper layer chip and a lower layer chip, wherein the two layers are made of polydimethylsiloxane materials; the chip has at least three parallel channel inlets, a common outlet, and a plurality of independent coaxial laminar flow channels connected to the inlets and outlets.
3. The method for preparing the ultra-thin cavity composite microfiber material based on the microfluidic technology according to claim 2, wherein the method comprises the following steps: the micro-fluidic chip is of a four-channel structure and consists of four parallel channel inlets, four independent coaxial laminar flow channels and a main outlet,
the four parallel channel inlets are a sheath fluid inlet, a sample fluid inlet, an inner cavity modification fluid inlet and a central inert fluid inlet,
the four independent coaxial laminar flow channels are a sheath flow fluid channel, a sample fluid channel, an inner cavity modification fluid channel and a central inert fluid channel from inside to outside in sequence,
the general outlet is a microfiber material outlet.
4. The preparation method of the ultrathin cavity composite microfiber material based on the microfluidic technology according to any one of claims 1 to 3, which is characterized by comprising the following specific steps:
(1) preparing mass volume concentration as follows: 1-5% g/ml of sample fluid; 1-5% g/ml of an inert fluid; 0.25-5% g/ml modifying fluid; 0.5-5% g/ml sheath flow fluid;
(2) adopting a micro-fluidic chip, controlling the flow rate of sheath flow fluid by using an air pressure pump, controlling the flow rates of fluids of other three passages by using a Harvard pump, and introducing inert fluid, modifying fluid, sample fluid and sheath flow fluid into the chip in sequence; the flow rates of the first three are respectively 0.1-5 mul/min, 0.1-5 mul/min and 1-40 mul/min; the pressure of the pneumatic pump of the sheath flow fluid is 20-200 mbar; preparing an ultrathin cavity composite microfiber material;
(3) and soaking the prepared ultrathin hollow cavity composite microfiber material in a solution capable of dissolving the outer layer material for 10-100min to obtain the ultrathin microfiber material.
5. The method for preparing an ultra-thin cavity composite microfiber material based on microfluidic technology as claimed in claim 4, wherein:
the sample fluid is a biological material that can be rapidly solidified;
the modified fluid is molecules and derivatives thereof with opposite charges with the sample fluid, or molecules and derivatives thereof capable of having certain interaction with the sample fluid, or molecules and derivatives thereof with strong adhesion;
the central inert fluid refers to inert water-soluble materials and derivatives thereof which do not react with the sample fluid;
the sheath flow fluid refers to a cross-linker solution of the sample fluid.
6. The method for preparing the ultra-thin cavity composite microfiber material based on the microfluidic technology according to claim 5, wherein the method comprises the following steps:
the sample fluid is one or a combination of the following: sodium alginate, polyethylene glycol diacrylate, chitosan and polylysine;
the modifying fluid is one or a combination of the following fluids: chitosan, chitin, polylysine, polydopamine, hyaluronic acid, agarose, collagen, laminin, fibronectin, type III collagen or serum expansion factor;
the central inert fluid is one or a combination of the following fluids: methyl cellulose, hydroxymethyl cellulose, polyvinyl alcohol or polyethylene oxide;
the sheath flow fluid is one or a combination of the following fluids: CaCl capable of rapidly crosslinking sodium alginate2Solutions or Ca2 +、Cu2+、Ba2+A solution of a multivalent ion; or a sodium tripolyphosphate solution capable of cross-linking chitosan; for polyethylene glycol diacrylate that does not require solution curing such as ultraviolet light to cure, the sheath fluid is an inert buffer solution.
7. The method for preparing the ultra-thin cavity composite microfiber material based on the microfluidic technology according to claim 4, wherein the method comprises the following steps: the solution for dissolving the outer layer material is phosphate solution capable of combining calcium ions, lysozyme solution capable of performing enzymolysis on the outer layer material, or other solutions capable of dissolving the outer layer material.
8. The method for preparing the ultra-thin cavity composite microfiber material based on the microfluidic technology according to claim 4, wherein the method comprises the following steps: before the preparation of the ultrathin cavity composite microfiber, the chip is introduced into a perfluorinated solution for surface hydrophobic treatment.
9. The method for preparing the ultra-thin cavity composite microfiber material based on the microfluidic technology according to claim 4, wherein the method comprises the following steps:
the ultrathin cavity composite microfiber material based on the microfluidic technology is prepared by the preparation method of the ultrathin cavity composite microfiber material, wherein the inner side of a cavity is modified;
during the process of preparing the microfibers, the density and thickness of the internal coating of the microfibers can be precisely controlled by controlling the concentration and flow rate of the modifying fluid.
10. The method for preparing the ultra-thin cavity composite microfiber material based on the microfluidic technology according to any one of claims 1 to 8, wherein the method comprises the following steps: the method preferably comprises the following steps:
(1) sodium alginate with mass volume concentration of 1-5% g/ml is used as a sample fluid in advance; methylcellulose with mass volume concentration of 0.5-5% g/ml is used as inert fluid; preparing a chitosan solution with the mass volume concentration of 0.5-10% g/ml by using acetic acid with the volume concentration of 0.25-5% as a modifying fluid; CaCl with mass volume concentration of 0.5-5% g/ml2As a sheath flow fluid;
(2) CaCl is controlled by adopting a microfluidic chip and an air pressure pump2The flow rate of the fluids in other three passages is controlled by a Harvard pump, and an inert fluid, namely methyl cellulose, a modified fluid, namely chitosan or a mixed solution of chitosan and methyl cellulose, sample fluid sodium alginate and sheath flow fluid CaCl are introduced into the chip in sequence2(ii) a The flow rates of the first three are respectively 0.1-5 mul/min, 0.1-5 mul/min and 1-40 mul/min; the pressure of the pneumatic pump of the sheath flow fluid is 20-200 mbar; preparing an ultrathin cavity composite microfiber material;
(3) and soaking the prepared ultrathin hollow-cavity composite microfiber material in a PBS (phosphate buffer solution) with the pH value of 7.4 for 10-100min to obtain the ultrathin microfiber material.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112708949A (en) * | 2020-12-23 | 2021-04-27 | 广西大学 | Preparation method of high-strength nano cellulose fiber based on microfluid assembly |
| CN113355754A (en) * | 2021-07-07 | 2021-09-07 | 中国计量大学 | Method for preparing multiphase fiber based on microfluidic-electrostatic spinning technology |
| CN114588956A (en) * | 2020-12-03 | 2022-06-07 | 中国科学院大连化学物理研究所 | Microfluidic chip glutathione local hydrophilic modification method based on laminar flow effect |
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