CN116117915A

CN116117915A - Quick scissors for cutting PVDF film before and after Western blotting rotary die

Info

Publication number: CN116117915A
Application number: CN202310247870.8A
Authority: CN
Inventors: 冯世庆; 姚雪; 杨玮奇; 齐章阳; 赵小庆; 张�荣; 张洋
Original assignee: Shandong University
Current assignee: Shandong University
Priority date: 2023-03-15
Filing date: 2023-03-15
Publication date: 2023-05-16

Abstract

The invention relates to the technical field of biological experiment equipment, in particular to a pair of quick scissors for cutting PVDF films before and after Western blotting rotary dies, which comprises an upper handle, a lower handle, a base and an L-shaped shearing knife, wherein the upper handle and the lower handle are arranged at intervals up and down, and the lower side of the right rear part of the upper handle and the upper side of the right rear part of the lower handle are hinged together. The invention has reasonable and compact structure and convenient use, the opening angle of the L-shaped shearing knife can be quickly adjusted through the transverse supporting component and the longitudinal supporting component, the positions of the blades in the transverse cutting component and the longitudinal cutting component can be quickly adjusted by correspondingly adjusting the extension lengths of the longitudinal adjusting rod and the transverse adjusting rod, the boundary of the target cutting area is completely overlapped with the boundary of the preset target area, and the preset target area is simply, quickly and accurately cut out at one time on the PVDF film; the opening angle of the L-shaped shearing knife is adjusted through the transverse supporting component and the longitudinal supporting component, so that PVDF film waste caused by overlarge shearing range can be effectively prevented, and the cutting area is flexible to select.

Description

Quick scissors for cutting PVDF film before and after Western blotting rotary die

Technical Field

The invention relates to the technical field of biological experimental equipment, in particular to a pair of quick scissors for cutting PVDF films before and after Western blotting rotary die.

Background

Immunoblotting (immunoblotting) is also known as Western blotting (Western blotting), and is a method for detecting a protein in a complex sample based on the specific binding of an antigen-antibody. Western blotting is an experimental method frequently used in molecular biology, biochemistry and immunogenetics, and is an analytical method capable of carrying out qualitative and semi-quantitative analysis on proteins; the method comprises the steps of staining a cell or biological tissue sample treated by gel electrophoresis through a specific antibody, and obtaining information of the expression condition of specific proteins in the analyzed cell or tissue by analyzing the staining position and the staining depth. And combining chemiluminescence detection, the expression quantity difference of the homologous proteins of a plurality of samples can be simultaneously compared. The main steps of Westernblotting include five steps of electrophoresis, transferring, sealing, hybridization and color development, wherein SDS-PAGE gel is required to be transferred to membrane after protein electrophoresis separation, namely, target protein is transferred to PVDF membrane for detection.

At present, the BlotCycler full-automatic protein hybridization instrument imported in the market can automatically perform the steps of sealing, hybridization, cleaning and the like after membrane transfer, but has the defects of high instrument price, huge antibody usage and the like compared with manual operation, and cannot be applied to most laboratories. In the manual operation, the PVDF film is high in price, and in order to save the experiment cost, the PVDF film with matched cutting size can be needed before die rotation according to the size of SDS-PAGE gel; after transferring the protein from the gel to the PVDF membrane, in order to achieve the purpose of incubating a plurality of primary antibodies simultaneously, the PVDF membrane needs to be cut according to the molecular weight of the target protein.

The Chinese patent document with the bulletin number of CN209440285U discloses a Western Blot membrane shearing device with a scale, which comprises a bottom plate and a knife rest, wherein the bottom plate is provided with the scale and an adjustable clamping groove, the scale is positioned on one side of the adjustable clamping groove, a positioning frame for placing SDS-PAGE gel of a membrane to be cut is formed by the scale and the adjustable clamping groove, and one side of the SDS-PAGE gel of the membrane to be cut is opposite to the scale and is fixed through the adjustable clamping groove. A WesternBlot Marker scale is designed on one side of the device, so that the position of the target protein can be clarified, and the target protein is prevented from being cut off; on the one hand, through the cutter, the film cutting is realized fast and accurately.

The Chinese patent document with the publication number of CN208663933U discloses a PVDF transfer film shearing device, which comprises a main shearing plate with a rectangular structure, a first shearing part arranged on the side part of the wide side line of the main shearing plate and a second shearing part arranged on the side part of the long side line of the main shearing plate, wherein a plurality of bases which are distributed in a rectangular shape are arranged at the bottom of the main shearing plate, a plurality of crisscross scale grooves are arranged on the main shearing plate, a first accommodating plate for accommodating the first shearing part is arranged on the side part of the length line of the main shearing plate adjacent to the first shearing part, a second accommodating plate for accommodating the first shearing part is arranged on the side part of the width line of the main shearing plate adjacent to the second shearing part, a first accommodating groove is arranged on the first accommodating plate, a second accommodating groove is arranged on the second accommodating plate, the first shearing part can move along the width direction of the main shearing plate, and the second shearing part can move along the length direction of the main shearing plate; the plurality of scale grooves comprise a plurality of wide scale grooves which are distributed at equal intervals along the width direction of the main shear plate and a plurality of long scale grooves which are distributed at equal intervals along the length direction of the main shear plate, and each of the wide scale grooves and the long scale grooves penetrates through the top of the main shear plate along the horizontal direction; the side wall of the main shearing plate is provided with an annular groove, the contour line of the annular groove is the same as that of the main shearing plate, and the first shearing component and the second shearing component are movably arranged in the annular groove; the first shearing component comprises a first sliding part, a first shearing part arranged on the first sliding part and a first rotating shaft part used for connecting the first sliding part and the first shearing part, the first sliding part comprises a first longitudinal sliding block, the first longitudinal sliding block is arranged in the annular groove, one end of the first longitudinal sliding block, which is far away from the inner side wall of the annular groove, extends outwards along the length direction of the main shearing plate, the first rotating shaft part comprises a first positioning rotating shaft and a first positioning pin arranged at the top of the first positioning rotating shaft, and one end of the first shearing part, which is close to the first longitudinal sliding block, can be arranged on the first positioning rotating shaft in a rotating way. The PVDF film molecular Marker and the target protein can slide according to the graduated scale so as to conveniently shear the corresponding PVDF film molecular Marker and the target protein, and the handles are arranged above the first cutter and the second cutter, so that the PVDF film molecular Marker and the target protein are conveniently sheared, and the PVDF film molecular Marker is conveniently pressed during shearing, thereby rapidly and accurately shearing the transfer film in a sharp mode.

Therefore, the cutting device used for the existing PVDF film has the following defects in the actual use process: aiming at PVDF films before and after film turning, the cutting process is complicated, and cutting is difficult to be carried out at one time; the shearing range is too large, so that only a preset target area on the PVDF film is difficult to cut, and the PVDF film is not wasted by one knife; the lack of flexibility in randomly cutting target proteins after die rotation is excessively cumbersome in cutting process, and it is difficult to accurately cut according to a predetermined target region (i.e., the specification and region desired by the experimenter, usually a plurality of regions)

Disclosure of Invention

The invention provides a pair of quick scissors for cutting PVDF film before and after Western blotting die rotation, which overcomes the defects of the prior art, and can effectively solve the problems that the PVDF film before and after die rotation is complex in process, difficult to cut at one time and lacks flexibility when randomly cutting target protein in the existing PVDF film cutting device; further solves the problems that PVDF film is wasted due to overlarge shearing range and accurate cutting is difficult to carry out according to a preset target area.

The technical scheme of the invention is realized by the following measures: a pair of quick scissors for cutting PVDF films before and after Western blotting rotary dies comprises an upper handle, a lower handle, a base and an L-shaped shearing knife, wherein the upper handle and the lower handle are arranged at intervals up and down; the right rear end of the upper handle is fixedly arranged on the outer side of the corner of the L-shaped shearing knife, the lower end of the front part of the L-shaped shearing knife is positioned in the first shearing groove, and the lower end of the left part of the L-shaped shearing knife is positioned in the second shearing groove; the upper part of the transverse supporting component is detachably provided with a transverse sliding rod, the transverse sliding rod corresponding to the inner side of the left part of the L-shaped shearing blade is provided with a guide sliding groove which is penetrated front and back, the L-shaped shearing blade corresponding to the upper part of the first shearing blade is provided with a longitudinal supporting component which can enable the L-shaped shearing blade to open a certain angle, and the upper part of the longitudinal supporting component is detachably provided with a longitudinal sliding rod of which the rear part is positioned in the guide sliding groove; the outer side of the longitudinal slide bar corresponding to the position between the transverse slide bar and the front part of the L-shaped shearing knife is sleeved with a first longitudinal cutting assembly capable of cutting the PVDF film in a sliding way along the longitudinal slide bar, the outer side of the transverse slide bar corresponding to the position between the longitudinal slide bar and the left part of the L-shaped shearing knife is sleeved with a first transverse cutting assembly capable of cutting the PVDF film in a sliding way along the transverse slide bar, and a target cutting area capable of cutting a preset target area on the PVDF film at one time is formed by a cutting path of the L-shaped shearing knife, a cutting path of the first longitudinal cutting assembly and a cutting path of the first transverse cutting assembly.

The following are further optimizations and/or improvements to the above-described inventive solution:

the longitudinal supporting assembly can comprise a longitudinal vertical shaft seat, a longitudinal fastening bolt, a longitudinal sliding block and a longitudinal adjusting bolt, wherein the outer side of the front end of the longitudinal sliding rod is provided with a longitudinal vertical shaft seat with a convex cross section, the middle part of the upper side of the longitudinal vertical shaft seat is provided with a longitudinal fastening screw hole which is penetrated internally and externally, the longitudinal fastening screw hole is internally provided with a longitudinal fastening bolt with the inner end propped against the outer side of the longitudinal sliding rod, a U-shaped longitudinal sliding block with a downward opening is arranged between the lower side of the longitudinal vertical shaft seat and the outer side of the upper part of the L-shaped shearing blade, the lower part of the front side of the longitudinal sliding block is fixedly provided with a longitudinal supporting table, the longitudinal supporting table is provided with a longitudinal adjusting screw hole which is penetrated vertically, the longitudinal adjusting screw hole is internally provided with a longitudinal adjusting bolt with the lower end propped against the upper side of the base, the front side of the L-shaped shearing blade corresponding to the lower part of the longitudinal sliding block is provided with a first sliding groove with a backward opening, the rear side of the L-shaped shearing blade corresponding to the first sliding groove is internally provided with a first guide block with the outer end fixedly installed together with the corresponding position of the inner side of the longitudinal sliding block.

The transverse supporting assembly can comprise a transverse vertical shaft seat, a transverse fastening bolt, a transverse sliding block and a transverse adjusting bolt, wherein the outer side of the left end of the transverse sliding rod is provided with the transverse vertical shaft seat, the cross section of the transverse vertical shaft seat is in a convex shape, the middle part of the upper side of the transverse vertical shaft seat is provided with a transverse fastening screw hole which is penetrated inwards and outwards, the transverse fastening screw hole is internally provided with the transverse fastening bolt, the inner end of the transverse fastening screw bolt is propped against the outer side of the transverse sliding rod, a U-shaped transverse sliding block with a downward opening is arranged between the lower side of the transverse vertical shaft seat and the outer side of the upper part of the L-shaped shearing knife, the transverse supporting table is fixedly provided with a transverse supporting table, the transverse supporting table is provided with the transverse adjusting screw hole which is penetrated upwards and downwards, the transverse adjusting screw hole is internally provided with the transverse adjusting bolt, the lower end of the transverse adjusting screw hole is propped against the upper side of the base, the left side of the L-shaped shearing knife corresponding to the lower side of the transverse sliding block is provided with a third sliding groove with a right opening, the right fourth sliding groove corresponding to the right side of the L-shaped shearing knife corresponding to the third sliding groove is arranged, and the fourth sliding groove and the third sliding groove are internally provided with a second guide block with the outer end fixedly installed together with the corresponding position of the inner side of the transverse sliding block.

The longitudinal sliding rod can be a cylindrical sliding rod, the longitudinal cutting assembly can comprise a longitudinal sliding sleeve, a longitudinal adjusting rod, a longitudinal locking bolt, a longitudinal cutter rod sleeve, a longitudinal cutter rod, a longitudinal cutter holder and a longitudinal cutter blade, the longitudinal sliding sleeve is sleeved outside the longitudinal sliding rod, the longitudinal adjusting sleeve is fixedly arranged on the left side of the longitudinal sliding sleeve, the longitudinal adjusting rod is sleeved in the longitudinal adjusting sleeve, the upper side of the left end of the longitudinal adjusting sleeve is provided with a longitudinal locking screw hole which is internally and externally penetrated, the longitudinal locking screw hole is internally provided with the longitudinal locking bolt, the inner end of the longitudinal locking screw hole is propped against the upper side of the longitudinal adjusting rod, the left end of the longitudinal adjusting rod is fixedly provided with the longitudinal cutter rod sleeve, the middle part of the longitudinal cutter rod sleeve is provided with a longitudinal guide cutter rod hole which is vertically penetrated, the longitudinal cutter rod is sleeved in the longitudinal guide cutter rod hole, the longitudinal cutter holder is fixedly arranged at the lower end of the longitudinal cutter rod, the lower side of the longitudinal cutter holder is provided with a longitudinal cutter blade groove which is downwards and longitudinally penetrated in front and back, and the longitudinal cutter blade with the lower end propped against the upper side of the base is arranged in the longitudinal cutter blade groove.

The transverse sliding rod is a cylindrical sliding rod, the transverse cutting assembly comprises a transverse sliding sleeve, a transverse adjusting rod, a transverse locking bolt, a transverse cutter rod sleeve, a transverse cutter rod, a transverse cutter holder and a transverse cutter blade, the transverse sliding sleeve is sleeved on the outer side of the transverse sliding rod, the transverse adjusting sleeve is fixedly installed on the front side of the transverse sliding sleeve, the transverse adjusting rod is sleeved in the transverse adjusting sleeve, the transverse locking bolt which is internally and externally penetrated is arranged on the upper side of the front end of the transverse adjusting sleeve, the transverse locking bolt which is internally and externally penetrated is arranged in the transverse locking screw hole, the transverse cutter rod sleeve, the front end of which is positioned behind the L-shaped shearing cutter, the middle part of the transverse cutter rod sleeve is provided with a guide transverse cutter rod hole which is vertically penetrated, the transverse cutter rod is sleeved in the guide transverse cutter rod hole, the transverse cutter holder is fixedly installed on the lower end of the transverse cutter rod, the transverse cutter blade groove which is downwards and horizontally penetrated is provided with an opening, and the transverse cutter blade which is internally and transversely butted with the upper side of the base is installed in the transverse cutter groove.

The longitudinal cutting assembly can further comprise a first pressure spring, a longitudinal anti-falling stop block and a second pressure spring, wherein the first pressure spring is arranged on the outer side of the longitudinal cutter bar corresponding to the position between the lower side of the longitudinal cutter bar sleeve and the upper side of the longitudinal cutter holder, and the longitudinal anti-falling stop block is detachably arranged at the upper end of the longitudinal cutter bar; the longitudinal adjusting rod corresponding to the position of the longitudinal locking bolt is provided with a first waist-shaped groove with an upward opening, the lower end of the longitudinal locking bolt is positioned in the first waist-shaped groove, the longitudinal adjusting sleeve corresponding to the right side of the longitudinal adjusting rod is internally provided with a second pressure spring, and a first copper sleeve or a first linear bearing is arranged between the longitudinal sliding sleeve and the longitudinal sliding rod.

The transverse cutting assembly can further comprise a third pressure spring, a transverse anti-falling stop block and a fourth pressure spring, wherein the third pressure spring is arranged on the outer side of the transverse cutter bar corresponding to the position between the lower side of the transverse cutter bar sleeve and the upper side of the transverse cutter holder, and the transverse anti-falling stop block is detachably arranged at the upper end of the transverse cutter bar; the transverse adjusting rod corresponding to the position of the transverse locking bolt is provided with a second waist-shaped groove with an upward opening, the lower end of the transverse locking bolt is positioned in the second waist-shaped groove, a fourth pressure spring is arranged in the transverse adjusting sleeve corresponding to the rear side of the transverse adjusting rod, and a second copper sleeve or a second linear bearing is arranged between the inner side of the transverse sliding sleeve and the outer side of the transverse sliding rod.

The outer side of the right part of the transverse sliding rod is slidably provided with a supporting component, the supporting component can comprise a supporting sliding sleeve, a fastening bolt, an inner sleeve rod, a fifth pressure spring, an outer sleeve, a flexible foot seat and a locking bolt, the supporting sliding sleeve is sleeved on the outer side of the right part of the transverse sliding rod, the upper side of the supporting sliding sleeve is provided with a fastening screw hole which is internally and externally communicated, and the fastening screw hole is internally provided with a fastening bolt with the inner end propped against the outer side of the transverse sliding rod; an inner sleeve rod is fixedly arranged on the lower side of the supporting sliding sleeve, an outer sleeve is sleeved on the outer side of the inner sleeve rod, a flexible foot seat with the lower end propped against the upper side of the base is fixedly arranged on the lower side of the outer sleeve, and a fifth pressure spring is arranged in the outer sleeve corresponding to the position between the flexible foot seat and the inner sleeve rod; the right side of the upper part of the outer sleeve is provided with a locking screw hole which is internally and externally communicated, an anti-drop groove with an outward opening and a waist shape is arranged on an inner sleeve rod corresponding to the position of the locking screw hole, and a locking bolt of which the inner end is arranged in the locking screw hole and the anti-drop groove are arranged in the locking screw hole.

The lower side of the right rear part of the upper handle and the upper side of the right rear part of the lower handle can be hinged together through a hinge shaft, a torsion spring can be arranged on the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle, the rear end and the right end of the L-shaped shearing knife are both detachably provided with a first baffle, and the right end of the transverse sliding rod is detachably provided with a second baffle.

The upper side of the base corresponding to the position between the first shearing groove and the second shearing groove can be provided with a coordinate grid, the upper side of the longitudinal slide bar corresponding to the position of the coordinate grid can be provided with a longitudinal graduated scale, the upper side of the transverse slide bar corresponding to the position of the coordinate grid can be provided with a transverse graduated scale, the upper side of the L-shaped shearing knife can be provided with two shearing graduated scales which are perpendicular to each other, and the zero points of the two shearing graduated scales are positioned at the corner positions of the L-shaped shearing knife.

The invention has reasonable and compact structure and convenient use, the opening angle of the L-shaped shearing knife can be quickly adjusted through the transverse supporting component and the longitudinal supporting component, the positions of the blades in the transverse cutting component and the longitudinal cutting component can be quickly adjusted by correspondingly adjusting the extension lengths of the longitudinal adjusting rod and the transverse adjusting rod, the boundary of the target cutting area is completely overlapped with the boundary of the preset target area, and the preset target area is simply, quickly and accurately cut out at one time on the PVDF film; the opening angle of the L-shaped shearing knife is adjusted through the transverse supporting component and the longitudinal supporting component, so that the PVDF film can be effectively prevented from being wasted due to overlarge shearing range, the cutting process is flexible, simple and easy to realize, and the cutting precision is high.

Drawings

Fig. 1 is a schematic top view of embodiment 1 of the present invention.

Fig. 2 is a schematic perspective view of fig. 1.

FIG. 3 is an enlarged schematic cross-sectional view of the support assembly of FIG. 1 at A-A.

Fig. 4 is a schematic perspective view of the longitudinal support assembly of fig. 1.

Fig. 5 is a schematic perspective view of the lateral support assembly of fig. 1.

Fig. 6 is a schematic view in partial cross-section of a perspective view of the longitudinal cutting assembly of fig. 1.

Fig. 7 is a schematic view in partial cross-section of a perspective view of the transverse cutting assembly of fig. 1.

The codes in the drawings are respectively: 1 is an upper handle, 2 is a lower handle, 3 is a base, 4 is an L-shaped shearing blade, 5 is a hinge shaft, 6 is a first shearing groove, 7 is a second shearing groove, 8 is a transverse vertical shaft seat, 9 is a transverse sliding rod, 10 is a guiding chute, 11 is a longitudinal sliding rod, 12 is a transverse sliding sleeve, 13 is a longitudinal vertical shaft seat, 14 is a longitudinal fastening bolt, 15 is a longitudinal sliding block, 16 is a longitudinal adjusting bolt, 17 is a longitudinal supporting table, 18 is a first chute, 19 is a fourth chute, 20 is a first guiding block, 21 is a longitudinal sliding sleeve, 22 is a longitudinal adjusting sleeve, 23 is a longitudinal adjusting rod, 24 is a longitudinal locking bolt, 25 is a longitudinal cutter bar sleeve, 26 is a longitudinal cutter bar, 27 is a longitudinal cutter bar, 28 is a longitudinal cutter bar, 29 is a first compression spring, 30 is a first waist-shaped groove, 31 is a second compression spring, 32 is a first linear bearing, 33 is a supporting sliding sleeve, 34 is a fastening bolt, 35 is an inner sleeve rod, 36 is a fifth pressure spring, 37 is an outer sleeve, 38 is a flexible foot seat, 39 is a locking bolt, 40 is a torsion spring, 41 is a first baffle, 42 is a second baffle, 43 is a coordinate grid, 44 is a longitudinal scale, 45 is a transverse scale, 46 is a shearing scale, 47 is a longitudinal anti-drop block, 48 is an anti-drop groove, 49 is a predetermined target area, 50 is a transverse fastening bolt, 51 is a transverse slider, 52 is a transverse adjusting bolt, 53 is a transverse supporting table, 54 is a second guide block, 55 is a transverse adjusting sleeve, 56 is a transverse adjusting rod, 57 is a transverse locking bolt, 58 is a transverse cutter bar sleeve, 59 is a transverse cutter bar, 60 is a transverse cutter bar, 62 is a third pressure spring, 63 is a transverse anti-drop block, 64 is a fourth pressure spring, 65 is a second waist-shaped groove, 66 is a second linear bearing.

Detailed Description

The present invention is not limited by the following examples, and specific embodiments can be determined according to the technical scheme and practical situations of the present invention.

In the present invention, for convenience of description, the description of the relative positional relationship of each component is described according to the layout manner of fig. 1 of the specification, for example: the positional relationship of the front, rear, upper, lower, left, right, etc. is determined in accordance with the layout direction of the drawings of the specification.

The invention is further described below with reference to examples and figures:

example 1: as shown in fig. 1, 2, 3, 4, 5, 6 and 7, the quick scissors for cutting the PVDF film before and after the Western blotting rotary die comprise an upper handle 1, a lower handle 2, a base 3 and an L-shaped shearing knife 4, wherein the upper handle 1 and the lower handle 2 are arranged at intervals from top to bottom, the lower side of the right rear part of the upper handle 1 and the upper side of the right rear part of the lower handle 2 are hinged together, the right rear end of the lower handle 2 and the outer side of the left lower corner of the base 3 are fixedly arranged together, the upper side of the front end of the base 3 is provided with a first shearing groove 6 with an upward opening, and the upper side of the left end of the base 3 is provided with a second shearing groove 7 with an upward opening and the front end communicated with the left end of the first shearing groove 6; the right rear end of the upper handle 1 is fixedly arranged with the outer side of the corner of the L-shaped shearing knife 4, the lower end of the front part of the L-shaped shearing knife 4 is positioned in the first shearing groove 6, and the lower end of the left part of the L-shaped shearing knife 4 is positioned in the second shearing groove 7; the L-shaped shearing knife 4 corresponding to the position above the second shearing groove 7 is slidably provided with a transverse supporting component which enables the L-shaped shearing knife 4 to open at a certain angle, the upper part of the transverse supporting component is detachably provided with a transverse sliding rod 9, the transverse sliding rod 9 corresponding to the position inside the left part of the L-shaped shearing knife 4 is provided with a guide sliding chute 10 which penetrates through the left part and the right part, the L-shaped shearing knife 4 corresponding to the position above the first shearing groove 6 is slidably provided with a longitudinal supporting component which enables the L-shaped shearing knife 4 to open at a certain angle, and the upper part of the longitudinal supporting component is detachably provided with a longitudinal sliding rod 11 of which the rear part is positioned in the guide sliding chute 10; the first longitudinal cutting assembly capable of cutting PVDF film in a sliding way is sleeved outside the longitudinal slide rod 11 corresponding to the position between the transverse slide rod 9 and the front part of the L-shaped shearing knife 4, the first transverse cutting assembly capable of cutting PVDF film in a sliding way along the transverse slide rod 9 is sleeved outside the transverse slide rod 9 corresponding to the position between the longitudinal slide rod 11 and the left part of the L-shaped shearing knife 4, and a target cutting area capable of cutting a preset target area 49 on PVDF film at one time is formed by the shearing path of the L-shaped shearing knife 4, the shearing path of the longitudinal cutting assembly and the shearing path of the transverse cutting assembly.

According to the requirement, the first shearing groove 6 and the second shearing groove 7 can form an L-shaped shearing groove matched with the shape of the L-shaped shearing knife 4, so that the lower part of the L-shaped shearing knife 4 can quickly enter the L-shaped shearing groove, the inner side of the lower end and the outer side of the lower end of the L-shaped shearing knife 4 are respectively provided with a guide fillet or a guide chamfer, the L-shaped shearing knife 4 can be opened upwards by pinching the left front part of the upper handle 1 and the lower handle 2, the L-shaped shearing knife 4 can be closed downwards and gradually enter the L-shaped shearing groove after being released, and the opening and closing operation of the L-shaped shearing knife 4 is easy, simple and quick; the first transverse cutting assembly can be realized through an outer sliding sleeve sleeved outside the transverse sliding rod 9, a movable longitudinal cutter bar arranged on the outer sliding sleeve and a cutting cutter arranged at the lower end of the longitudinal cutter bar, or can be realized through the outer sliding sleeve, the longitudinal cutter bar capable of adjusting the length in a telescopic way (a plurality of sections of longitudinal cutter bars sleeved together) and the cutting cutter, or can be directly realized based on a linear guide rail in the prior art, namely, the transverse sliding rod 9 is realized through the guide rail, a sliding block on the guide rail is matched with the longitudinal cutter bar and the cutting cutter to realize the sliding cutting function, the structure of the first longitudinal cutting assembly can be the same as that of the first transverse cutting assembly, and different structures can be selected, so that the cutting edge of the cutting cutter can reliably prop against the boundary of a target cutting area on the PVDF film to perform sliding cutting; when the longitudinal slide bar 11 and the transverse slide bar 9 slide to the preset positions, the lower ends of the transverse supporting component and the longitudinal supporting component can form a support with the upper side of the base 3, so that the L-shaped shearing knife 4 can be opened at a certain angle, the right front part and the left rear part of the L-shaped shearing knife 4 are prevented from contacting the upper side of the inner side wall of the L-shaped shearing groove, the shearing range of the L-shaped shearing knife 4 is prevented from being overlarge, the residual PVDF film is also cut by one knife, waste is caused (in order to completely cut the front boundary and the left boundary of the preset target area 49, the size of the target cutting area is slightly larger than that of the preset target area 49), and in addition, in order to quickly fix the transverse supporting component and the longitudinal supporting component at the preset positions on the L-shaped shearing knife 4, the transverse supporting component and the longitudinal supporting component can be connected with a fastening jackscrew in a threaded manner; the sliding connection between the transverse supporting component and the longitudinal supporting component and the L-shaped shearing blade 4 can be realized through a sliding block and a guide rail structure in a linear guide rail in the prior art or directly through a linear guide rail or a dovetail groove and a dovetail-shaped sliding block or a T-shaped groove, a T-shaped sliding block and the like, and in addition, in order to open the L-shaped shearing blade 4 by a certain angle, the transverse supporting component and the longitudinal supporting component can be realized through arranging a supporting column or a supporting wedge block or a plunger or a clamping table, and the transverse supporting component and the longitudinal supporting component can be of the same structure or of different structures.

In the use process, the positions of the transverse supporting component and the longitudinal supporting component are correspondingly adjusted according to the size and the shape of the preset target area 49, so that the L-shaped shearing blade 4 is opened by a certain angle, and then the relative positions of the transverse supporting component and the longitudinal supporting component and the L-shaped shearing blade 4 are fixed; then, after lifting the L-shaped shearing blade 4, placing the predetermined target area 49 to be sheared on the PVDF film at the lower left corner of the upper side of the base 3, aligning the lower left corner of the predetermined target area 49 with the corner of the L-shaped shearing groove, then loosening the upper handle 1 and the lower handle 2 (at this time, the left boundary and the front boundary of the target shearing area coincide with the left boundary and the front boundary of the predetermined target area 49, the right boundary of the target shearing area is located at the right side of the predetermined target area 49, the rear boundary of the target shearing area is located at the rear of the predetermined target area 49, i.e., the target shearing area is slightly larger than the predetermined target area 49), pressing the L-shaped shearing blade 4 downwards, the left boundary and the front boundary of the predetermined target area 49 can be sheared by friction between the inner side of the L-shaped shearing blade 4 and the inner side of the L-shaped shearing groove, and then sliding the first transverse cutting assembly and the first longitudinal cutting assembly up and down respectively, so that the remaining two edges of the predetermined target area 49 can be sheared rapidly. The invention has reasonable and compact structure and convenient use, and can quickly adjust the opening angle of the L-shaped shearing blade 4 through the transverse supporting component and the longitudinal supporting component to ensure that the target cutting area is slightly larger than the preset target area 49, and the preset target area 49 is simply, quickly and accurately cut out at one time on the PVDF film.

The rapid scissors for cutting the PVDF film before and after the Western blotting die rotation can be further optimized or/and improved according to actual needs:

example 2: as shown in fig. 1, 2 and 4, the longitudinal supporting component comprises a longitudinal vertical shaft seat 13, a longitudinal fastening bolt 14, a longitudinal sliding block 15 and a longitudinal adjusting bolt 16, the outer side of the front end of the longitudinal sliding rod 11 is provided with the longitudinal vertical shaft seat 13 with a convex cross section, the middle part of the upper side of the longitudinal vertical shaft seat 13 is provided with a longitudinal fastening screw hole which is penetrated internally and externally, the longitudinal fastening screw hole is internally provided with the longitudinal fastening bolt 14 with the inner end propped against the outer side of the longitudinal sliding rod 11, a U-shaped longitudinal sliding block 15 with a downward opening is arranged between the lower side of the longitudinal vertical shaft seat 13 and the outer side of the upper part of the L-shaped shearing blade 4, the lower part of the front side of the longitudinal sliding block 15 is fixedly provided with a longitudinal supporting table 17, the longitudinal adjusting screw hole 16 with the lower end propped against the upper side of the base 3 is arranged in the longitudinal adjusting screw hole, the front side of the L-shaped shearing blade 4 corresponding to the lower part of the longitudinal sliding block 15 is provided with a first sliding groove 18 with a forward opening, the rear side of the L-shaped shearing blade 4 corresponding to the first sliding groove 18 is provided with a second sliding groove with a first guiding block 20 with the outer end fixedly arranged corresponding to the inner side of the longitudinal sliding block 15.

In the use process, the longitudinal slide bar 11 is firstly slid to the vicinity of the preset position according to the position size of the preset target area 49, then the longitudinal adjusting bolt 16 is screwed to be adjusted, the opening angle between the base 3 and the L-shaped shearing knife 4 is adjusted by adjusting the length of the longitudinal adjusting bolt extending out of the lower side of the longitudinal supporting table 17, so that the right front part of the L-shaped shearing knife 4 is separated from the notch of the first shearing groove 6, the shearing range of the L-shaped shearing knife 4 is just slightly larger than the preset target area 49, the preset target area 49 can be completely cut, PVDF film outside the preset target area 49 is not excessively cut, the integrity of the residual PVDF film is ensured as much as possible, the influence on the subsequent use due to excessive breakage of the residual PVDF film is avoided, and the experiment cost is saved; the longitudinal adjusting bolt 16 can also limit the relative position between the longitudinal sliding block 15 and the L-shaped shearing blade 4, so that when the L-shaped shearing blade 4 is opened to a preset angle, the longitudinal sliding block 15 slides to the corner position of the L-shaped shearing blade 4 due to self weight to influence the relative position of the longitudinal sliding rod 11, and the longitudinal cutting assembly is prevented from deviating from the preset cutting position; the longitudinal sliding block 15 and the L-shaped shearing blade 4 can form a linear guide rail in the prior art, so that the sliding installation between the longitudinal sliding rod 11 and the L-shaped shearing blade 4 is realized; when the L-shaped shearing blade 4 is opened, the first sliding groove 18 and the second sliding groove are matched with the first guide block 20 arranged on the inner side of the longitudinal sliding block 15, so that the longitudinal sliding block 15 can be effectively prevented from being separated from the L-shaped shearing blade 4. According to the requirement, in order to facilitate the experimenter to quickly screw the longitudinal adjusting bolt 16 by hand, the longitudinal adjusting bolt 16 can be realized by screwing the bolt or the butterfly bolt by hand in the prior known technology; the structure of the vertical shaft seat 13 is known in the prior art, and is mainly used for installing and fixing shaft ends.

Example 3: as shown in fig. 1, 2 and 5, the transverse supporting assembly may include a transverse vertical shaft seat 8, a transverse fastening bolt 50, a transverse sliding block 51 and a transverse adjusting bolt 52, the outer side of the left end of the transverse sliding rod 9 is provided with the transverse vertical shaft seat 8 with a convex cross section, the middle of the upper side of the transverse vertical shaft seat 8 is provided with a transverse fastening screw hole penetrated internally and externally, the transverse fastening screw hole is internally provided with the transverse fastening bolt 50 with the inner end propped against the outer side of the transverse sliding rod 9, a U-shaped transverse sliding block 51 with a downward opening is arranged between the lower side of the transverse vertical shaft seat 8 and the outer side of the upper part of the L-shaped shearing blade 4, the lower part of the transverse sliding block 51 is fixedly provided with a transverse supporting table 53, the transverse supporting table 53 is provided with a transverse adjusting screw hole penetrated vertically, the transverse adjusting screw 52 with the lower end propped against the upper side of the base 3 is arranged in the transverse adjusting screw hole, the left side of the L-shaped shearing blade 4 corresponding to the lower part of the transverse sliding block 51 is provided with a third chute with a leftward opening, the right fourth chute 19 corresponding to the right side of the L-shaped shearing blade 4 at the third chute position is arranged in the fourth chute 19 and the third chute is internally provided with a second guide block 54 with the outer end fixedly installed together with the inner corresponding position of the inner side of the transverse sliding block 51.

In the use process, the transverse slide bar 9 is firstly slid to the vicinity of the preset position according to the position size of the preset target area 49, then the transverse adjusting bolt 52 is screwed to adjust the opening angle between the base 3 and the L-shaped shearing knife 4 by adjusting the length of the transverse adjusting bolt extending out of the lower side of the transverse supporting table 53, so that the left upper part of the L-shaped shearing knife 4 is separated from the notch of the second shearing groove 7, the shearing range of the L-shaped shearing knife 4 is just slightly larger than that of the preset target area 49, the preset target area 49 can be completely cut but the PVDF film outside the preset target area 49 is not excessively cut, the integrity of the residual PVDF film is ensured as much as possible, the influence on the subsequent use due to excessive breakage of the residual PVDF film is avoided, and the experiment cost is saved; the transverse adjusting bolt 52 can also limit the relative position between the transverse sliding block 51 and the L-shaped shearing blade 4, so that when the L-shaped shearing blade 4 is opened to a preset angle, the transverse sliding block 51 slides to the corner position of the L-shaped shearing blade 4 due to the dead weight to influence the relative position of the transverse sliding rod 9, and the transverse cutting assembly is prevented from deviating from the preset cutting position; the transverse sliding block 51 and the L-shaped shearing knife 4 can form a linear guide rail in the prior art, so that sliding installation between the transverse sliding rod 9 and the L-shaped shearing knife 4 is realized; when the L-shaped shearing blade 4 is opened, the third sliding groove and the fourth sliding groove 19 are matched with the second guide block 54 arranged on the inner side of the transverse sliding block 51, so that the transverse sliding block 51 can be effectively prevented from being separated from the L-shaped shearing blade 4. According to the requirement, in order to facilitate the experimenter to quickly screw the transverse adjusting bolt 52 by hand, the transverse adjusting bolt 52 can be realized by manually screwing the bolt or the butterfly bolt in the prior known technology; because the outer diameter of the transverse sliding rod 9 is larger than that of the longitudinal sliding rod 11, the size of the transverse vertical shaft seat 8 in the transverse supporting assembly is larger than that of the transverse vertical shaft seat 8 in the transverse supporting assembly, and the structure of the transverse vertical shaft seat 8 is a prior known technology and is mainly used for installing and fixing shaft ends.

Example 4: as shown in fig. 1, 2 and 6, the longitudinal slide bar 11 may be a cylindrical slide bar, the longitudinal cutting assembly includes a longitudinal sliding sleeve 21, a longitudinal adjusting sleeve 22, a longitudinal adjusting bar 23, a longitudinal locking bolt 24, a longitudinal knife bar sleeve 25, a longitudinal knife bar 26, a longitudinal knife holder 27 and a longitudinal knife blade 28, the outside of the longitudinal slide bar 11 is sleeved with the longitudinal sliding sleeve 21, the left side of the longitudinal sliding sleeve 21 is fixedly provided with the longitudinal adjusting sleeve 22, the longitudinal adjusting bar 23 is sleeved in the longitudinal adjusting sleeve 22, the upper side of the left end of the longitudinal adjusting sleeve 22 is provided with a longitudinal locking screw hole which is internally and externally penetrated, the longitudinal locking screw hole is internally provided with a longitudinal locking bolt 24, the inner end of the longitudinal locking screw hole is propped against the upper side of the longitudinal adjusting bar 23, the left end of the longitudinal knife bar sleeve 23 is fixedly provided with a longitudinal knife bar sleeve 25, the left end of the longitudinal knife bar sleeve 25 is provided with a longitudinal guiding knife bar hole which is vertically penetrated, the longitudinal knife bar 26 is sleeved in the longitudinal guiding knife bar hole, the lower end of the longitudinal knife bar 26 is fixedly provided with the longitudinal knife holder 27, the lower side of the longitudinal knife holder 27 is provided with a longitudinal knife blade groove which is opened downwards and is longitudinally penetrated forwards and backwards, and the longitudinal knife blade 28 of which is arranged in the longitudinal knife blade groove and the lower end is propped against the upper side of the base 3.

By means of the arrangement, the longitudinal sliding sleeve 21 can slide and rotate along the outer side of the cylindrical longitudinal sliding rod 11, the longitudinal sliding sleeve 21 is rotated to enable the longitudinal cutter bar 26 to be perpendicular to the upper side face of the base 3, the longitudinal adjusting rod 23 is pushed and pulled to adjust the relative position of the longitudinal cutter bar 26 left and right, therefore the cutting edge of the longitudinal blade 28 is perpendicular to and coincides with the right boundary of the preset target area 49, accurate cutting of the right boundary of the preset target area 49 is facilitated, the longitudinal locking bolt 24 can be used for quickly fixing the relative position between the longitudinal adjusting rod 23 and the longitudinal adjusting sleeve 22, and the influence of movement of the longitudinal adjusting rod 23 on cutting accuracy in the PVDF film cutting process is prevented; the longitudinal cutter bar sleeve 25 is used for supporting the longitudinal cutter bar 26, the longitudinal guide cutter bar hole has a guiding function, the cutting path of the longitudinal cutter blade 28 is prevented from deviating from the right boundary of the preset target area 49 due to the rotation of the longitudinal cutter bar 26 in the process of cutting PVDF film, the cutting precision is affected, the cross section of the longitudinal cutter bar sleeve can be drum-shaped or square, pentagon or hexagonal according to the requirement, the shape of the longitudinal cutter bar 26 is matched with that of the longitudinal guide cutter bar hole, and the drum-shaped longitudinal cutter bar is used in the embodiment.

In the use process, the longitudinal cutter bar 26 is correspondingly adjusted according to the right boundary position of the preset target area 49, so that the cutting edge of the longitudinal cutter blade 28 is vertical to the PVDF film and completely coincides with the right boundary of the preset target area 49 (even if the target cutting area completely coincides with the preset target area 49), and the right boundary of the preset target area 49 can be quickly and accurately cut off according to the preset size requirement by pushing the longitudinal cutter bar 26 forwards and backwards; to ensure sharpness of the longitudinal blade 28, the longitudinal blade 28 may be a disposable replaceable longitudinal blade, so that the fixing between the longitudinal blade 28 and the longitudinal adjustment sleeve 22 may be achieved by threaded fasteners, so as to allow quick assembly and disassembly for replacement of the longitudinal blade 28; depending on the length dimension of the longitudinal sliding sheath 21, the length of the longitudinal blade 28 generally needs to be greater than the length of the longitudinal sliding sheath 21 in order to ensure that the longitudinal blade 28 is able to fully cut the predetermined target area 49.

Example 5: as shown in fig. 1, 2 and 7, the transverse sliding rod 9 is a cylindrical sliding rod, the transverse cutting assembly comprises a transverse sliding sleeve 12, a transverse adjusting sleeve 55, a transverse adjusting rod 56, a transverse locking bolt 57, a transverse cutter rod sleeve 58, a transverse cutter rod 59, a transverse cutter holder 60 and a transverse cutter blade 61, the transverse sliding sleeve 12 is sleeved outside the transverse sliding rod 9, the transverse adjusting sleeve 55 is fixedly arranged on the front side of the transverse sliding sleeve 12, the transverse adjusting rod 56 is sleeved in the transverse adjusting sleeve 55, an internally and externally penetrating transverse locking screw hole is formed in the upper side of the front end of the transverse adjusting sleeve 55, a transverse locking bolt 57 with the inner end propped against the upper side of the transverse adjusting rod 56 is arranged in the transverse locking screw hole, a transverse cutter rod sleeve 58 with the front end positioned behind the L-shaped shear cutter 4 is fixedly arranged at the front end of the transverse adjusting rod 56, a guide transverse cutter rod 59 hole penetrating up and down is formed in the middle of the transverse cutter rod sleeve 58, the transverse cutter rod 59 is sleeved in the guide transverse cutter rod 59, the transverse cutter holder 60 is fixedly arranged at the lower end of the transverse cutter rod 59, a transverse cutter blade slot penetrating downwards and the transverse cutter blade is left and right, and right transversely cutter blades 61 are arranged in the lower end of the transverse cutter holder 60.

By means of the arrangement, the transverse sliding sleeve 12 can slide and rotate along the outer side of the cylindrical transverse sliding rod 9, the transverse sliding sleeve 12 is rotated to enable the transverse cutter bar 59 to be perpendicular to the upper side face of the base 3, the transverse adjusting rod 56 is pushed and pulled to adjust the relative position of the transverse cutter bar 59 forwards and backwards, therefore the cutting edge of the transverse blade 61 is perpendicular to and coincides with the rear boundary of the preset target area 49, the rear boundary of the preset target area 49 is conveniently and accurately cut, the transverse locking bolt 57 can be used for rapidly fixing the relative position between the transverse adjusting rod 56 and the transverse adjusting sleeve 55, and the influence of the movement of the transverse adjusting rod 56 on cutting accuracy in the PVDF film cutting process is prevented; the transverse cutter bar sleeve 58 is used for supporting the transverse cutter bar 59, the transverse guide cutter bar hole has a guiding function, the cutting path of the transverse cutter blade 61 is prevented from deviating from the right boundary of the preset target area 49 due to the rotation of the transverse cutter bar 59 in the process of cutting PVDF film, the cutting precision is affected, the cross section of the transverse cutter bar sleeve 58 can be drum-shaped or square, pentagon or hexagonal according to the requirement, the shape of the transverse cutter bar 59 is matched with that of the transverse guide cutter bar hole, and the drum-shaped transverse cutter bar 59 is used in the embodiment.

In the use process, the position of the transverse cutter bar 59 of the transverse cutting assembly is correspondingly adjusted according to the position of the rear boundary of the preset target area 49, so that the cutting edge of the transverse cutter blade 61 is vertical to the PVDF film and completely coincides with the rear boundary of the preset target area 49 (thereby completely coinciding the target cutting area with the preset target area 49), and the rear boundary of the preset target area 49 can be quickly and accurately cut according to the preset size requirement by pushing the transverse cutter bar 59 left and right; to ensure sharpness of the transverse blade 61, the transverse blade 61 may be a disposable replaceable transverse blade 61, so that the fixation between the transverse blade 61 and the transverse adjustment sleeve 55 may be achieved by threaded fasteners for quick assembly disassembly and replacement of the transverse blade 61; depending on the length dimension of the lateral sliding sheath 12, the length of the lateral blade 61 generally needs to be greater than the length of the lateral sliding sheath 12 in order to ensure that the lateral blade 61 is able to fully cut the predetermined target area 49; since the outer diameter of the transverse slide bar 9 is larger than that of the transverse slide bar 9, the inner and outer diameter sizes of the transverse slide sleeve 12 are larger than those of the longitudinal slide sleeve 21.

Example 6: as shown in fig. 1, 2 and 6, the longitudinal cutting assembly further comprises a first compression spring 29, a longitudinal anti-falling stop 47 and a second compression spring 31, wherein the first compression spring 29 is arranged on the outer side of a longitudinal cutter bar 26 corresponding to the position between the lower side of the longitudinal cutter bar sleeve 25 and the upper side of the longitudinal cutter bar 27, and the longitudinal anti-falling stop 47 is detachably arranged at the upper end of the longitudinal cutter bar 26; the longitudinal adjusting rod 23 corresponding to the position of the longitudinal locking bolt 24 is provided with a first waist-shaped groove 30 with an upward opening, and the lower end of the longitudinal locking bolt 24 is positioned in the first waist-shaped groove 30; a second compression spring 31 is arranged in the longitudinal adjustment sleeve 22 corresponding to the right side position of the longitudinal adjustment rod 23; a first copper sleeve or first linear bearing 32 is arranged between the longitudinal slide sleeve 21 and the longitudinal slide bar 11.

In the use process, the first pressure spring 29 can enable the longitudinal blade 28 to be propped against the upper side of the base 3 (or PVDF film) all the time, so that stable cutting is facilitated; when the longitudinal locking bolt 24 is loosened, the second pressure spring 31 can enable the longitudinal adjusting rod 23 to automatically pop up leftwards, so that an experimenter can conveniently and quickly enable the cutting edge of the longitudinal blade 28 to coincide with the right boundary of the preset target area 49 in the process of pushing the longitudinal adjusting rod 23 rightwards, the adjustment efficiency is improved, at the moment, the lower end of the longitudinal locking bolt 24 is blocked at the bottom of the first waist-shaped groove 30, the longitudinal adjusting rod 23 can be prevented from popping up under the action of the leftward elastic force of the second pressure spring 31, and the first waist-shaped groove 30 can prevent the longitudinal adjusting sleeve 22 and the longitudinal adjusting rod 23 from being separated and separated under the action of the spring restoring force of the second pressure spring 31; the first copper sleeve or the first linear bearing 32 can enable the longitudinal sliding sleeve 21 to slide along the outer side of the longitudinal sliding rod 11 more smoothly, more stably and more wear-resistant, and in order to improve the lubrication effect, the first copper sleeve can use a graphite self-lubricating copper sleeve; the longitudinal anti-disengagement block 47 can prevent the longitudinal cutter bar 26 from being separated from the longitudinal cutter bar sleeve 25 under the restoring force of the spring of the first compression spring 29, and the longitudinal anti-disengagement block 47 can be mounted on the longitudinal cutter bar 26 by a screw or a screw fastener in the known art.

Example 7: as shown in fig. 1, 2 and 7, the transverse cutting assembly may further include a third compression spring 62, a transverse anti-drop stop 63 and a fourth compression spring 64, wherein the third compression spring 62 is arranged on the outer side of the transverse cutter bar 59 corresponding to the position between the lower side of the transverse cutter bar sleeve 58 and the upper side of the transverse cutter bar 60, and the transverse anti-drop stop 63 is detachably mounted on the upper end of the transverse cutter bar 59; the transverse adjusting rod 56 corresponding to the position of the transverse locking bolt 57 is provided with a second waist-shaped groove 65 with an upward opening, the lower end of the transverse locking bolt 57 is positioned in the second waist-shaped groove 65, the transverse adjusting sleeve 55 corresponding to the rear side of the transverse adjusting rod 56 is internally provided with a fourth pressure spring 64, and a second copper sleeve or a second linear bearing 66 is arranged between the inner side of the transverse sliding sleeve 12 and the outer side of the transverse sliding rod 9.

In the use process, the third pressure spring 62 can enable the transverse blade 61 to be always propped against the upper side of the base 3 (or PVDF film), which is more beneficial to stable cutting; when the transverse locking bolt 57 is loosened, the fourth pressure spring 64 can enable the transverse adjusting rod 56 to automatically pop up forwards, so that an experimenter can conveniently and quickly enable the cutting edge of the transverse blade 61 to coincide with the rear boundary of a preset target area in the process of pushing the transverse adjusting rod 56 backwards, the adjustment efficiency is improved, at the moment, the lower end of the transverse locking bolt 57 is blocked at the bottom of the second waist-shaped groove 65, the transverse adjusting rod 56 can be prevented from popping up under the action of the leftward elastic force of the fourth pressure spring 64, and the second waist-shaped groove 65 can prevent the transverse adjusting sleeve 55 and the transverse adjusting rod 56 from being separated and separated under the action of the spring restoring force of the fourth pressure spring 64; the second copper sleeve or the second linear bearing 66 can enable the lateral sliding sleeve 12 to slide along the outer side of the lateral sliding rod 9 more smoothly, more stably and more wear-resistant, and in order to improve the lubrication effect, the second copper sleeve can use a graphite self-lubricating copper sleeve; the lateral drop-preventing block 63 prevents the lateral cutter bar 59 from being separated from the lateral cutter bar sleeve 58 by the restoring force of the third compression spring 62, and the lateral drop-preventing block 63 may be mounted on the lateral cutter bar 59 by a screw or threaded fastener as known in the art.

Example 8: as shown in fig. 1, 2 and 3, a supporting component is arranged on the outer side of the right part of the transverse slide bar 9 in a sliding way, the supporting component comprises a supporting slide sleeve 33, a fastening bolt 34, an inner sleeve rod 35, a fifth pressure spring 36, an outer sleeve 37, a flexible foot seat 38 and a locking bolt 39, the supporting slide sleeve 33 is sleeved on the outer side of the right part of the transverse slide bar 9, a fastening screw hole which is internally and externally communicated is arranged on the upper side of the supporting slide sleeve 33, and the fastening bolt 34 with the inner end propped against the outer side of the transverse slide bar 9 is arranged in the fastening screw hole; an inner sleeve rod 35 is fixedly arranged on the lower side of the support sliding sleeve 33, an outer sleeve 37 is sleeved on the outer side of the inner sleeve rod 35, a flexible foot seat 38 with the lower end propped against the upper side of the base 3 is fixedly arranged on the lower side of the outer sleeve 37, and a fifth pressure spring 36 is arranged in the outer sleeve 37 corresponding to the position between the flexible foot seat 38 and the inner sleeve rod 35; the right side of the upper part of the outer sleeve 37 is provided with a locking screw hole which is internally and externally penetrated, the inner sleeve rod 35 corresponding to the position of the locking screw hole is provided with an outward opening anti-disengaging groove 48, and the locking screw hole is internally provided with a locking bolt 39 with an inner end and the anti-disengaging groove 48.

By the arrangement, the inner end of the locking bolt 39 props against the bottom of the anti-falling groove 48, so that the relative position between the inner sleeve rod 35 and the outer sleeve 37 can be fixed; the inner sleeve rod 35, the outer sleeve 37 and the flexible foot seats 38 can temporarily fix the PVDF film when forming a support, prevent the PVDF film from moving in the cutting process, and avoid the deformation of the transverse sliding rod 9 or the longitudinal sliding rod 11 of the transverse cutting assembly and the longitudinal cutting assembly in the sliding process from affecting the cutting precision; the fifth pressure spring 36 can enable the flexible foot base 38 to automatically extend downwards, so that the adjusting efficiency is improved; in addition, when the base 3 is made of ferromagnetic material, the flexible foot 38 can also be realized by a magnet fixedly mounted on the lower side of the outer sleeve 37, and a stable support is formed by magnetic force.

Example 9: as shown in fig. 1 and 2, the lower side of the right rear part of the upper handle 1 and the upper side of the right rear part of the lower handle 2 are hinged together through a hinge shaft 5, a torsion spring 40 is arranged on the outer side of the hinge shaft 5 corresponding to the position between the upper handle 1 and the lower handle 2, a first baffle 41 is detachably arranged at the rear end and the right end of the L-shaped shearing blade 4, and a second baffle 42 is detachably arranged at the right end of the transverse sliding rod 9. By the arrangement, the L-shaped shearing knife 4 can be automatically closed, and simultaneously a downward shearing force is applied to the L-shaped shearing knife 4, so that PVDF films can be automatically sheared, the cutting efficiency is improved, and labor is saved; the torsion spring 40 can also enable the lower end of the transverse supporting component and the lower end of the longitudinal supporting component to be always propped against the upper side surface of the base 3, so that the L-shaped shearing knife 4 is prevented from shaking; the first blocking piece 41 is used for preventing the longitudinal sliding block 15 from being separated from the L-shaped shearing blade 4, the second blocking piece 42 is used for preventing the supporting component from being separated from the transverse sliding rod 9, and the first blocking piece 41 and the second blocking piece 42 can be detachably mounted through threaded fasteners.

Example 10: as shown in fig. 1 and 2, a coordinate grid 43 is arranged on the upper side of the base 3 corresponding to the position between the first shearing groove 6 and the second shearing groove 7, a longitudinal graduated scale 44 is arranged on the upper side of the longitudinal sliding rod 11 corresponding to the position of the coordinate grid 43, a transverse graduated scale 45 is arranged on the upper side of the transverse sliding rod 9 corresponding to the position of the coordinate grid 43, two mutually perpendicular shearing graduated scales 42 are arranged on the upper side of the L-shaped shearing knife 4, and zero points of the two shearing graduated scales 42 are positioned at the corner positions of the L-shaped shearing knife 4.

By so doing, the coordinate grid 43 facilitates positioning of the predetermined target area 49 and rapid adjustment of the relative positions of the lateral and longitudinal cutting assemblies to the predetermined target area 49; the size of the target cropping zone can be quickly and accurately adjusted by the longitudinal scale 44, the transverse scale 45 and the shear scale 42.

The technical characteristics form the embodiment of the invention, have stronger adaptability and implementation effect, and can increase or decrease unnecessary technical characteristics according to actual needs so as to meet the requirements of different situations.

Claims

1. The quick scissors for cutting the PVDF film before and after the Western blotting rotary die are characterized by comprising an upper handle, a lower handle, a base and an L-shaped shearing knife, wherein the upper handle and the lower handle are arranged at intervals from top to bottom; the right rear end of the upper handle is fixedly arranged on the outer side of the corner of the L-shaped shearing knife, the lower end of the front part of the L-shaped shearing knife is positioned in the first shearing groove, and the lower end of the left part of the L-shaped shearing knife is positioned in the second shearing groove; the upper part of the transverse supporting component is detachably provided with a transverse sliding rod, the transverse sliding rod corresponding to the inner side of the left part of the L-shaped shearing blade is provided with a guide sliding groove which is penetrated front and back, the L-shaped shearing blade corresponding to the upper part of the first shearing blade is provided with a longitudinal supporting component which can enable the L-shaped shearing blade to open a certain angle, and the upper part of the longitudinal supporting component is detachably provided with a longitudinal sliding rod of which the rear part is positioned in the guide sliding groove; the outer side of the longitudinal slide bar corresponding to the position between the transverse slide bar and the front part of the L-shaped shearing knife is sleeved with a first longitudinal cutting assembly capable of cutting the PVDF film in a sliding way along the longitudinal slide bar, the outer side of the transverse slide bar corresponding to the position between the longitudinal slide bar and the left part of the L-shaped shearing knife is sleeved with a first transverse cutting assembly capable of cutting the PVDF film in a sliding way along the transverse slide bar, and a target cutting area capable of cutting a preset target area on the PVDF film at one time is formed by a cutting path of the L-shaped shearing knife, a cutting path of the first longitudinal cutting assembly and a cutting path of the first transverse cutting assembly.

2. The quick scissors for cutting the PVDF film before and after the Western blotting rotary die according to claim 1, wherein the longitudinal supporting component comprises a longitudinal vertical shaft seat, a longitudinal fastening bolt, a longitudinal sliding block and a longitudinal adjusting bolt, the outer side of the front end of a longitudinal sliding rod is provided with the longitudinal vertical shaft seat with a convex cross section, the middle part of the upper side of the longitudinal vertical shaft seat is provided with a longitudinal fastening screw hole penetrating inside and outside, the longitudinal fastening screw hole is internally provided with the longitudinal fastening bolt of which the inner end is propped against the outer side of the longitudinal sliding rod, a U-shaped longitudinal sliding block with a downward opening is arranged between the lower side of the longitudinal vertical shaft seat and the outer side of the upper part of the L-shaped shearing knife, the lower part of the front side of the longitudinal sliding block is fixedly provided with a longitudinal supporting table, the longitudinal adjusting bolt of which the lower end is propped against the upper side of a base is arranged on the longitudinal supporting table, the front side of the L-shaped shearing knife corresponding to the lower part of the longitudinal sliding block is provided with a first sliding groove with a backward opening, the second sliding groove with the inner side of the opening corresponding to the lower side of the L-shaped shearing knife, the second sliding groove and the first sliding groove are both provided with a first guide block with a fixed outer end and a corresponding position to the inner side of the longitudinal sliding block;

Or/and, the transverse supporting component comprises a transverse vertical shaft seat, a transverse fastening bolt, a transverse sliding block and a transverse adjusting bolt, wherein the outer side of the left end of the transverse sliding rod is provided with the transverse vertical shaft seat, the cross section of the transverse vertical shaft seat is in a convex shape, the middle part of the upper side of the transverse vertical shaft seat is provided with a transverse fastening screw hole which is penetrated inwards and outwards, the transverse fastening screw hole is internally provided with the transverse fastening bolt, the inner end of the transverse fastening screw bolt is propped against the outer side of the transverse sliding rod, a U-shaped transverse sliding block with a downward opening is arranged between the lower side of the transverse vertical shaft seat and the outer side of the upper part of the L-shaped shearing knife, the lower side of the transverse sliding block is fixedly provided with a transverse supporting table, the transverse supporting table is provided with the transverse adjusting screw hole which is penetrated upwards and downwards, the transverse adjusting screw hole is internally provided with the transverse adjusting bolt, the left side of the lower end of the transverse adjusting screw hole is propped against the upper side of the base, the left side of the L-shaped shearing knife corresponding to the lower side of the transverse sliding block is provided with a fourth sliding groove with a rightward opening, and the fourth sliding groove corresponding to the right side of the L-shaped shearing knife at the position of the third sliding groove is internally provided with a second guide block, and the outer end of the fourth sliding groove and the third sliding groove is fixedly arranged together with the corresponding position of the inner side of the transverse sliding groove.

3. The quick scissors for cutting out the PVDF film before and after the Western blotting rotary die according to claim 1 or 2, which is characterized in that the longitudinal slide bar is a cylindrical slide bar, the longitudinal cutting assembly comprises a longitudinal sliding sleeve, a longitudinal adjusting rod, a longitudinal locking bolt, a longitudinal cutter rod sleeve, a longitudinal cutter rod, a longitudinal cutter seat and a longitudinal cutter blade, the longitudinal sliding sleeve is sleeved outside the longitudinal slide bar, the longitudinal adjusting sleeve is fixedly arranged at the left side of the longitudinal sliding sleeve, the longitudinal adjusting rod is sleeved in the longitudinal adjusting sleeve, the upper side of the left end of the longitudinal adjusting sleeve is provided with a longitudinal locking screw hole which is internally and externally penetrated, the longitudinal locking screw hole is internally provided with a longitudinal cutter rod sleeve with the inner end propped against the upper side of the longitudinal adjusting rod, the left end of the longitudinal adjusting rod is fixedly provided with a longitudinal cutter rod sleeve with the left end positioned at the right side of the L-shaped shearing cutter, the longitudinal guiding cutter rod hole is vertically penetrated in the middle of the longitudinal cutter rod sleeve, the longitudinal cutter rod sleeve is fixedly sleeved with the longitudinal cutter rod, the lower end of the longitudinal cutter rod is fixedly provided with the longitudinal cutter seat, the lower side of the longitudinal cutter seat is provided with a longitudinal cutter blade groove which is downwards opened and is penetrated in front and back, the longitudinal cutter blade groove is penetrated in the longitudinal direction, and the lower end of the longitudinal cutter seat is propped against the upper side of the base;

Or/and, the horizontal slide bar is cylindrical slide bar, horizontal cutting assembly includes horizontal slip cap, horizontal adjusting sleeve, horizontal regulation pole, horizontal locking bolt, horizontal cutter arbor cover, horizontal cutter arbor, horizontal blade holder and horizontal blade, horizontal slide bar outside cover is equipped with horizontal slip cap, horizontal adjusting sleeve is fixed mounting in horizontal slip cap front side, horizontal adjusting sleeve endotheca is equipped with horizontal regulation pole, horizontal locking screw that link up inside and outside is equipped with in horizontal adjusting sleeve front end upside, be equipped with the horizontal locking bolt of inner and horizontal regulation pole upside offset in the horizontal locking screw, horizontal regulation pole front end fixed mounting has the horizontal cutter arbor cover that the front end is located L type shearing sword rear, horizontal cutter arbor cover middle part is equipped with the direction horizontal cutter arbor hole that link up from top to bottom, the downthehole cover of direction horizontal cutter arbor has horizontal cutter arbor, horizontal cutter arbor lower extreme fixed mounting has horizontal blade holder, horizontal cutter arbor downside is equipped with the opening and horizontal blade groove that link up from side about, install the lower extreme and base upside offset in the horizontal cutter arbor groove.

4. The pair of quick scissors for cutting out the PVDF film before and after the Western blotting rotary die according to claim 3, wherein the longitudinal cutting assembly further comprises a first pressure spring, a longitudinal anti-falling stop block and a second pressure spring, the first pressure spring is arranged on the outer side of the longitudinal cutter bar corresponding to the position between the lower side of the longitudinal cutter bar sleeve and the upper side of the longitudinal cutter bar, and the longitudinal anti-falling stop block is detachably arranged at the upper end of the longitudinal cutter bar; the longitudinal adjusting rod corresponding to the position of the longitudinal locking bolt is provided with a first waist-shaped groove with an upward opening, the lower end of the longitudinal locking bolt is positioned in the first waist-shaped groove, the longitudinal adjusting sleeve corresponding to the right side of the longitudinal adjusting rod is internally provided with a second pressure spring, and a first copper sleeve or a first linear bearing is arranged between the longitudinal sliding sleeve and the longitudinal sliding rod;

Or/and, the transverse cutting assembly further comprises a third pressure spring, a transverse anti-falling stop block and a fourth pressure spring, wherein the third pressure spring is arranged on the outer side of the transverse cutter bar corresponding to the position between the lower side of the transverse cutter bar sleeve and the upper side of the transverse cutter holder, and the transverse anti-falling stop block is detachably arranged at the upper end of the transverse cutter bar; the transverse adjusting rod corresponding to the position of the transverse locking bolt is provided with a second waist-shaped groove with an upward opening, the lower end of the transverse locking bolt is positioned in the second waist-shaped groove, a fourth pressure spring is arranged in the transverse adjusting sleeve corresponding to the rear side of the transverse adjusting rod, and a second copper sleeve or a second linear bearing is arranged between the inner side of the transverse sliding sleeve and the outer side of the transverse sliding rod.

5. The pair of quick scissors for cutting out PVDF films before and after Western blotting rotary dies according to claim 1, 2 or 4, wherein a supporting component is arranged on the outer side of the right part of the transverse sliding rod in a sliding manner, the supporting component comprises a supporting sliding sleeve, a fastening bolt, an inner sleeve rod, a fifth pressure spring, an outer sleeve, a flexible foot seat and a locking bolt, the supporting sliding sleeve is sleeved on the outer side of the right part of the transverse sliding rod, the upper side of the supporting sliding sleeve is provided with a fastening screw hole which is internally and externally communicated, and the fastening bolt of which the inner end is propped against the outer side of the transverse sliding rod is arranged in the fastening screw hole; an inner sleeve rod is fixedly arranged on the lower side of the supporting sliding sleeve, an outer sleeve is sleeved on the outer side of the inner sleeve rod, a flexible foot seat with the lower end propped against the upper side of the base is fixedly arranged on the lower side of the outer sleeve, and a fifth pressure spring is arranged in the outer sleeve corresponding to the position between the flexible foot seat and the inner sleeve rod; the right side of the upper part of the outer sleeve is provided with a locking screw hole which is internally and externally communicated, an anti-drop groove with an outward opening and a waist shape is arranged on an inner sleeve rod corresponding to the position of the locking screw hole, and a locking bolt of which the inner end is arranged in the locking screw hole and the anti-drop groove are arranged in the locking screw hole.

6. The pair of quick scissors for cutting out the PVDF film before and after the Western blotting rotary die according to claim 3, wherein the outer side of the right part of the transverse slide bar is slidably provided with a supporting component, the supporting component comprises a supporting slide sleeve, a fastening bolt, an inner sleeve rod, a fifth pressure spring, an outer sleeve, a flexible foot seat and a locking bolt, the outer side of the right part of the transverse slide bar is sleeved with the supporting slide sleeve, the upper side of the supporting slide sleeve is provided with a fastening screw hole which is internally and externally penetrated, and the fastening screw hole is internally provided with a fastening bolt with the inner end propped against the outer side of the transverse slide bar; an inner sleeve rod is fixedly arranged on the lower side of the supporting sliding sleeve, an outer sleeve is sleeved on the outer side of the inner sleeve rod, a flexible foot seat with the lower end propped against the upper side of the base is fixedly arranged on the lower side of the outer sleeve, and a fifth pressure spring is arranged in the outer sleeve corresponding to the position between the flexible foot seat and the inner sleeve rod; the right side of the upper part of the outer sleeve is provided with a locking screw hole which is internally and externally communicated, an anti-drop groove with an outward opening and a waist shape is arranged on an inner sleeve rod corresponding to the position of the locking screw hole, and a locking bolt of which the inner end is arranged in the locking screw hole and the anti-drop groove are arranged in the locking screw hole.

7. The pair of shortcut scissors for cutting out the PVDF film before and after the Western blotting rotary die according to claim 1, 2, 4 or 6, wherein the lower side of the right rear part of the upper handle and the upper side of the right rear part of the lower handle are hinged together through a hinge shaft, a torsion spring is arranged at the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle, a first baffle is detachably arranged at the rear end and the right end of the L-shaped shearing knife, and a second baffle is detachably arranged at the right end of the transverse sliding rod; or/and, the upper side of the base corresponding to the position between the first shearing groove and the second shearing groove is provided with a coordinate grid, the upper side of the longitudinal slide bar corresponding to the position of the coordinate grid is provided with a longitudinal graduated scale, the upper side of the transverse slide bar corresponding to the position of the coordinate grid is provided with a transverse graduated scale, the upper side of the L-shaped shearing knife is provided with two shearing graduated scales which are mutually perpendicular, and the zero points of the two shearing graduated scales are positioned at the corner positions of the L-shaped shearing knife.

8. The pair of quick scissors for cutting out the PVDF film before and after the Western blotting rotary die according to claim 3, wherein the lower side of the right rear part of the upper handle and the upper side of the right rear part of the lower handle are hinged together through a hinge shaft, a torsion spring is arranged at the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle, a first baffle is detachably arranged at the rear end and the right end of the L-shaped shearing blade, and a second baffle is detachably arranged at the right end of the transverse slide bar; or/and, the upper side of the base corresponding to the position between the first shearing groove and the second shearing groove is provided with a coordinate grid, the upper side of the longitudinal slide bar corresponding to the position of the coordinate grid is provided with a longitudinal graduated scale, the upper side of the transverse slide bar corresponding to the position of the coordinate grid is provided with a transverse graduated scale, the upper side of the L-shaped shearing knife is provided with two shearing graduated scales which are mutually perpendicular, and the zero points of the two shearing graduated scales are positioned at the corner positions of the L-shaped shearing knife.

9. The pair of quick scissors for cutting out the PVDF film before and after the Western blotting rotary die according to claim 5, wherein the lower side of the right rear part of the upper handle and the upper side of the right rear part of the lower handle are hinged together through a hinge shaft, a torsion spring is arranged at the outer side of the hinge shaft corresponding to the position between the upper handle and the lower handle, a first baffle is detachably arranged at the rear end and the right end of the L-shaped shearing blade, and a second baffle is detachably arranged at the right end of the transverse slide bar; or/and, the upper side of the base corresponding to the position between the first shearing groove and the second shearing groove is provided with a coordinate grid, the upper side of the longitudinal slide bar corresponding to the position of the coordinate grid is provided with a longitudinal graduated scale, the upper side of the transverse slide bar corresponding to the position of the coordinate grid is provided with a transverse graduated scale, the upper side of the L-shaped shearing knife is provided with two shearing graduated scales which are mutually perpendicular, and the zero points of the two shearing graduated scales are positioned at the corner positions of the L-shaped shearing knife.